Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005047

Physical Location & Seq

Genomic Coordinates Forward (+)

27610814 .. 27615281

Gene Structure 4468 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1183600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGGCAAAAGAAGATGGAGTTGTGGTGGTCAGAAATGGCGATAGTGAAGTCTCCCTTCCACCAATCTCAGCTGGAACTGAATACAATACTAAAGAACTTGCAGATGATAATCTTACTGATGATCATCCAAAGGTTCCTGGATGGTTTTCTGAGTATTGTCCGATATGGCCAGGGCAAGCCCACTTTTTGAAGGTAGAAAAAATCTTATTTCAAGGGAGGTCCGAGTATCAAAGTATGATGGTATTTCAGTCATCAGCATATGGCAAGGTTTTTATTCTGGATGGAGCACTCCAACTCACTGAGAAGGATGAGTTTGCTTACCAAGAAATGATTACACATCTTCCTCTTTGCTCCATTCCGAACCCAAACAAGGTTTTACTCATTGGGGGAGGAGATGGCGGTATCTTAAGGGAGATTTCTCGACACTCTTCTGTCCAGCATATTGATATTTGCGAAATAGACACCATGTTGGTTGATGTGTACAAGAAATATTTTCCTAGTGTTGCTATTGGATACGAGGACCCTCGGGTGACCCTTCACGTTAAAGATGGTTCTGCATTTCTTAAGTCTGTCCCAGAAGGAACATATGATGCGATAATAGTGGATGCGTTTGATCCTATTAGGCCGGATCATGAGCTTCACAAGACTCCCTTCTTTGAGTTGGTGGCAAAAGCCCTCCTACCTGGCGGAGTCATGTGTATTCAAGCAGAGAGCATATGGTTTCGTTCTTTAGACATTGAGCAACTCATGGTCAAATGTCGACCAATATTCAAAGGCTCTGTTCGGTATGCATGGACAATTGTTCCCGCATATCCAAGTGGGGTGATTGGTTTCTTGCTTTGCTCCACGGAGGGGCCACTGGTGGATTTCAGAAATCCTGTCAACCCAATAGACCCCAACAATAGTTATGGGGTTGCAAAAGAGCCCCTGAAATTTTACAACACTGAGGTGCACATGGCTGCATTCTGTTTGCCATCTTTTGCAAAGAAGGTGAATAATGGGTCTAAAATGAATGGAGTTGGAACGCTGGCAGCTGATGAGGCAAAACACAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

352

Amino Acids

38.93

Weight (kDa)

5.41

Isoelectric Point (pI)

38.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	47 - 101	1.3e-20	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	104 - 291	2.4e-65	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005047)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23820 AT1G23820 AT1G70310
fragaria_vesca	FvH4_4g34680
malus_domestica	MD13G1090400.v1.1 MD14G1183600.v1.1 MD16G1091000.v1.1
prunus_persica	Prupe.1G255300_v2.0.a1 Prupe.5G176100_v2.0.a1
pyrus_communis	pycom13g07870 pycom16g07770
rosa_chinensis	RchiOBHm_Chr4g0444031 RchiOBHm_Chr7g0193881
rosa_laevigata	RLG00000004238 RLG00000005863
rosa_multiflora	Rmu_sc0015522.1_g000006
rosa_roxburghii	Rroxscaffold_3G00261760 Rroxscaffold_5G00384600
rosa_rugosa	Rorug04G0349100 Rorug07G0012500
rosa_samantha	Rh4AG409900 Rh4BG421000 Rh4CG435300 Rh4DG416200 Rh7AG139200 Rh7BG139600 Rh7CG143100 Rh7DG140700
rosa_wichuraiana	Rw4G035210 Rw7G011880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	760
AciI	CCGC	3 cut(s)	399, 687, 807
AclWI	GGATC	2 cut(s)	608, 636
AcoI	YGGCCR	1 cut(s)	167
AcsI	RAATTY	1 cut(s)	932
AfaI	GTAC	1 cut(s)	482
AfiI	CCNNNNNNNGG	2 cut(s)	370, 852
AflII	CTTAAG	2 cut(s)	406, 563
AgsI	TTSAA	4 cut(s)	190, 212, 704, 772
AjnI	CCWGG	3 cut(s)	136, 169, 682
AleI	CACNNNNGTG	1 cut(s)	947
AluBI	AGCT	3 cut(s)	71, 637, 1034
AluI	AGCT	3 cut(s)	71, 637, 1034
Alw21I	GWGCWC	2 cut(s)	289, 954
Alw26I	GTCTC	1 cut(s)	55
Alw44I	GTGCAC	1 cut(s)	950
AlwI	GGATC	2 cut(s)	608, 636
Ama87I	CYCGRG	1 cut(s)	525
AoxI	GGCC	3 cut(s)	167, 623, 854
ApaLI	GTGCAC	1 cut(s)	950
ApeKI	GCWGC	2 cut(s)	959, 1031
ApoI	RAATTY	1 cut(s)	932
AspS9I	GGNCC	3 cut(s)	219, 520, 854
AsuHPI	GGTGA	3 cut(s)	541, 835, 1003
AvaI	CYCGRG	1 cut(s)	525
AvaII	GGWCC	2 cut(s)	219, 520
BaeGI	GKGCMC	1 cut(s)	954
BalI	TGGCCA	1 cut(s)	169
BanII	GRGCYC	1 cut(s)	927
Bbv12I	GWGCWC	2 cut(s)	289, 954
BbvI	GCAGC	2 cut(s)	946, 1043
BccI	CCATC	7 cut(s)	8, 135, 232, 275, 389, 542, 982
BciT130I	CCWGG	3 cut(s)	138, 171, 684
BciVI	GTATCC	1 cut(s)	506
BclI	TGATCA	1 cut(s)	121
BcoDI	GTCTC	1 cut(s)	55
BfaI	CTAG	1 cut(s)	498
BfrI	CTTAAG	2 cut(s)	406, 563
BfuI	GTATCC	1 cut(s)	506
BisI	GCNGC	2 cut(s)	960, 1032
BlsI	GCNGC	2 cut(s)	961, 1033
Bme1390I	CCNGG	3 cut(s)	138, 171, 684
Bme18I	GGWCC	2 cut(s)	219, 520
BmeT110I	CYCGRG	1 cut(s)	525
BmgT120I	GGNCC	3 cut(s)	219, 520, 854
BmiI	GGNNCC	3 cut(s)	135, 522, 855
BmrFI	CCNGG	3 cut(s)	138, 171, 684
BmsI	GCATC	2 cut(s)	580, 595
BsaBI	GATNNNNATC	2 cut(s)	108, 123
BsaJI	CCNNGG	3 cut(s)	170, 524, 846
BsaXI	ACNNNNNCTCC	2 cut(s)	9, 39
Bsc4I	CCNNNNNNNGG	2 cut(s)	370, 852
Bse1I	ACTGG	1 cut(s)	864
Bse8I	GATNNNNATC	2 cut(s)	108, 123
BseBI	CCWGG	3 cut(s)	138, 171, 684
BseDI	CCNNGG	3 cut(s)	170, 524, 846
BseGI	GGATG	5 cut(s)	124, 146, 286, 313, 610
BseJI	GATNNNNATC	2 cut(s)	108, 123
BseLI	CCNNNNNNNGG	2 cut(s)	370, 852
BseMII	CTCAG	4 cut(s)	81, 141, 291, 936
BseNI	ACTGG	1 cut(s)	864
BseRI	GAGGAG	1 cut(s)	405
BseSI	GKGCMC	1 cut(s)	954
BseXI	GCAGC	2 cut(s)	946, 1043
BshFI	GGCC	3 cut(s)	169, 625, 856
BsiHKAI	GWGCWC	2 cut(s)	289, 954
BsiHKCI	CYCGRG	1 cut(s)	525
BsiSI	CCGG	1 cut(s)	626
BslFI	GGGAC	1 cut(s)	557
BslI	CCNNNNNNNGG	2 cut(s)	370, 852
BsmAI	GTCTC	1 cut(s)	55
BsmFI	GGGAC	1 cut(s)	557
BsmI	GAATGC	1 cut(s)	962
BsnI	GGCC	3 cut(s)	169, 625, 856
BsoBI	CYCGRG	1 cut(s)	525
Bsp1286I	GDGCHC	3 cut(s)	289, 927, 954
Bsp1407I	TGTACA	1 cut(s)	480
Bsp143I	GATC	3 cut(s)	121, 613, 628
BspACI	CCGC	3 cut(s)	399, 687, 807
BspANI	GGCC	3 cut(s)	169, 625, 856
BspCNI	CTCAG	4 cut(s)	80, 142, 292, 937
BspHI	TCATGA	1 cut(s)	631
BspLI	GGNNCC	3 cut(s)	135, 522, 855
BspPI	GGATC	2 cut(s)	608, 636
BspTI	CTTAAG	2 cut(s)	406, 563
BsrGI	TGTACA	1 cut(s)	480
BsrI	ACTGG	1 cut(s)	864
BssECI	CCNNGG	3 cut(s)	170, 524, 846
BssMI	GATC	3 cut(s)	121, 613, 628
Bst2UI	CCWGG	3 cut(s)	138, 171, 684
Bst6I	CTCTTC	1 cut(s)	433
BstAFI	CTTAAG	2 cut(s)	406, 563
BstAUI	TGTACA	1 cut(s)	480
BstC8I	GCNNGC	2 cut(s)	177, 1029
BstDEI	CTNAG	4 cut(s)	67, 150, 300, 945
BstDSI	CCRYGG	1 cut(s)	846
BstEII	GGTNACC	1 cut(s)	529
BstF5I	GGATG	5 cut(s)	124, 146, 286, 313, 610
BstKTI	GATC	3 cut(s)	124, 616, 631
BstMAI	GTCTC	1 cut(s)	55
BstMBI	GATC	3 cut(s)	121, 613, 628
BstMWI	GCNNNNNNNGC	1 cut(s)	1040
BstNI	CCWGG	3 cut(s)	138, 171, 684
BstPI	GGTNACC	1 cut(s)	529
BstSCI	CCNGG	3 cut(s)	136, 169, 682
BstSLI	GKGCMC	1 cut(s)	954
BstV1I	GCAGC	2 cut(s)	946, 1043
BsuI	GTATCC	1 cut(s)	506
BsuRI	GGCC	3 cut(s)	169, 625, 856
BtgI	CCRYGG	1 cut(s)	846
BtsCI	GGATG	5 cut(s)	124, 146, 286, 313, 610
BtsIMutI	CAGTG	3 cut(s)	297, 857, 942
Cac8I	GCNNGC	2 cut(s)	177, 1029
CciI	TCATGA	1 cut(s)	631
Cfr13I	GGNCC	3 cut(s)	219, 520, 854
Csp6I	GTAC	1 cut(s)	481
CviAII	CATG	6 cut(s)	466, 632, 694, 748, 792, 955
CviQI	GTAC	1 cut(s)	481
DdeI	CTNAG	4 cut(s)	67, 150, 300, 945
DpnI	GATC	3 cut(s)	123, 615, 630
DpnII	GATC	3 cut(s)	121, 613, 628
EaeI	YGGCCR	1 cut(s)	167
Eam1104I	CTCTTC	1 cut(s)	433
EarI	CTCTTC	1 cut(s)	433
EciI	GGCGGA	1 cut(s)	702
Eco24I	GRGCYC	1 cut(s)	927
Eco47I	GGWCC	2 cut(s)	219, 520
Eco88I	CYCGRG	1 cut(s)	525
Eco91I	GGTNACC	1 cut(s)	529
EcoO109I	RGGNCCY	1 cut(s)	520
EcoO65I	GGTNACC	1 cut(s)	529
EcoRII	CCWGG	3 cut(s)	136, 169, 682
EcoT22I	ATGCAT	1 cut(s)	793
EcoT38I	GRGCYC	1 cut(s)	927
FaeI	CATG	6 cut(s)	469, 635, 697, 751, 795, 958
FalI	AAGNNNNNCTT	4 cut(s)	39, 71, 635, 667
FaqI	GGGAC	1 cut(s)	557
FatI	CATG	6 cut(s)	465, 631, 693, 747, 791, 954
FauI	CCCGC	1 cut(s)	814
FauNDI	CATATG	3 cut(s)	259, 586, 716
FbaI	TGATCA	1 cut(s)	121
FblI	GTMKAC	1 cut(s)	760
Fnu4HI	GCNGC	2 cut(s)	960, 1032
FokI	GGATG	5 cut(s)	111, 153, 293, 320, 617
FriOI	GRGCYC	1 cut(s)	927
Fsp4HI	GCNGC	2 cut(s)	960, 1032
FspBI	CTAG	1 cut(s)	498
GluI	GCNGC	2 cut(s)	960, 1032
HaeIII	GGCC	3 cut(s)	169, 625, 856
HapII	CCGG	1 cut(s)	626
Hin1II	CATG	6 cut(s)	469, 635, 697, 751, 795, 958
HincII	GTYRAC	2 cut(s)	761, 883
HindII	GTYRAC	2 cut(s)	761, 883
HinfI	GANTC	2 cut(s)	646, 690
HpaII	CCGG	1 cut(s)	626
HphI	GGTGA	3 cut(s)	541, 835, 1003
Hpy166II	GTNNAC	4 cut(s)	481, 761, 883, 952
Hpy188I	TCNGA	6 cut(s)	32, 151, 162, 223, 360, 872
Hpy188III	TCNNGA	3 cut(s)	278, 420, 632
Hpy8I	GTNNAC	4 cut(s)	481, 761, 883, 952
HpyAV	CCTTC	7 cut(s)	65, 184, 298, 545, 572, 661, 982
HpyCH4IV	ACGT	1 cut(s)	540
HpyCH4V	TGCA	7 cut(s)	101, 557, 791, 917, 952, 962, 983
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1040
HpyF3I	CTNAG	4 cut(s)	67, 150, 300, 945
HpySE526I	ACGT	1 cut(s)	540
Hsp92II	CATG	6 cut(s)	469, 635, 697, 751, 795, 958
Ksp22I	TGATCA	1 cut(s)	121
Kzo9I	GATC	3 cut(s)	121, 613, 628
LmnI	GCTCC	3 cut(s)	284, 356, 848
Lsp1109I	GCAGC	2 cut(s)	946, 1043
LweI	GCATC	2 cut(s)	580, 595
MaeI	CTAG	1 cut(s)	498
MaeII	ACGT	1 cut(s)	540
MaeIII	GTNAC	1 cut(s)	529
MalI	GATC	3 cut(s)	123, 615, 630
MboI	GATC	3 cut(s)	121, 613, 628
MboII	GAAGA	3 cut(s)	23, 332, 420
MfeI	CAATTG	1 cut(s)	798
MhlI	GDGCHC	3 cut(s)	289, 927, 954
MlsI	TGGCCA	1 cut(s)	169
MluCI	AATT	2 cut(s)	798, 932
MluNI	TGGCCA	1 cut(s)	169
MlyI	GAGTC	2 cut(s)	640, 699
MmeI	TCCRAC	2 cut(s)	316, 1000
MnlI	CCTC	9 cut(s)	210, 354, 383, 511, 534, 686, 844, 940, 1033
Mox20I	TGGCCA	1 cut(s)	169
Mph1103I	ATGCAT	1 cut(s)	793
MscI	TGGCCA	1 cut(s)	169
MseI	TTAA	3 cut(s)	407, 543, 564
MslI	CAYNNNNRTG	1 cut(s)	947
Msp20I	TGGCCA	1 cut(s)	169
MspA1I	CMGCKG	2 cut(s)	71, 1034
MspCI	CTTAAG	2 cut(s)	406, 563
MspI	CCGG	1 cut(s)	626
MspR9I	CCNGG	3 cut(s)	138, 171, 684
MunI	CAATTG	1 cut(s)	798
Mva1269I	GAATGC	1 cut(s)	962
MvaI	CCWGG	3 cut(s)	138, 171, 684
MwoI	GCNNNNNNNGC	1 cut(s)	1040
NdeI	CATATG	3 cut(s)	259, 586, 716
NdeII	GATC	3 cut(s)	121, 613, 628
NlaIII	CATG	6 cut(s)	469, 635, 697, 751, 795, 958
NlaIV	GGNNCC	3 cut(s)	135, 522, 855
NmuCI	GTSAC	1 cut(s)	529
NsiI	ATGCAT	1 cut(s)	793
OliI	CACNNNNGTG	1 cut(s)	947
PagI	TCATGA	1 cut(s)	631
PctI	GAATGC	1 cut(s)	962
PfoI	TCCNGGA	1 cut(s)	136
PkrI	GCNGC	2 cut(s)	961, 1033
PleI	GAGTC	2 cut(s)	640, 698
PpsI	GAGTC	2 cut(s)	640, 698
PpuMI	RGGWCCY	1 cut(s)	520
Psp5II	RGGWCCY	1 cut(s)	520
Psp6I	CCWGG	3 cut(s)	136, 169, 682
PspEI	GGTNACC	1 cut(s)	529
PspGI	CCWGG	3 cut(s)	136, 169, 682
PspN4I	GGNNCC	3 cut(s)	135, 522, 855
PspPI	GGNCC	3 cut(s)	219, 520, 854
PspPPI	RGGWCCY	1 cut(s)	520
PvuII	CAGCTG	2 cut(s)	71, 1034
RsaI	GTAC	1 cut(s)	482
RsaNI	GTAC	1 cut(s)	481
RseI	CAYNNNNRTG	1 cut(s)	947
SalI	GTCGAC	1 cut(s)	759
SaqAI	TTAA	3 cut(s)	407, 543, 564
SatI	GCNGC	2 cut(s)	960, 1032
Sau3AI	GATC	3 cut(s)	121, 613, 628
Sau96I	GGNCC	3 cut(s)	219, 520, 854
SchI	GAGTC	2 cut(s)	640, 699
ScrFI	CCNGG	3 cut(s)	138, 171, 684
SduI	GDGCHC	3 cut(s)	289, 927, 954
SfaNI	GCATC	2 cut(s)	580, 595
SinI	GGWCC	2 cut(s)	219, 520
SmiMI	CAYNNNNRTG	1 cut(s)	947
SmlI	CTYRAG	2 cut(s)	406, 563
SmoI	CTYRAG	2 cut(s)	406, 563
Sse9I	AATT	2 cut(s)	798, 932
SsiI	CCGC	3 cut(s)	399, 687, 807
SspI	AATATT	2 cut(s)	491, 768
SspMI	CTAG	1 cut(s)	498
StyD4I	CCNGG	3 cut(s)	136, 169, 682
TaiI	ACGT	1 cut(s)	543
TaqI	TCGA	2 cut(s)	421, 760
TasI	AATT	2 cut(s)	798, 932
TatI	WGTACW	1 cut(s)	480
Tru1I	TTAA	3 cut(s)	407, 543, 564
Tru9I	TTAA	3 cut(s)	407, 543, 564
TscAI	CASTG	3 cut(s)	304, 864, 949
TseFI	GTSAC	1 cut(s)	529
TseI	GCWGC	2 cut(s)	959, 1031
Tsp45I	GTSAC	1 cut(s)	529
TspDTI	ATGAA	1 cut(s)	1025
TspGWI	ACGGA	1 cut(s)	863
TspRI	CASTG	3 cut(s)	304, 864, 949
Vha464I	CTTAAG	2 cut(s)	406, 563
VneI	GTGCAC	1 cut(s)	950
VpaK11BI	GGWCC	2 cut(s)	219, 520
XapI	RAATTY	1 cut(s)	932
XmiI	GTMKAC	1 cut(s)	760
XspI	CTAG	1 cut(s)	498
Zsp2I	ATGCAT	1 cut(s)	793

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.