Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0005047

Physical Location & Seq

Genomic Coordinates Reverse (-)

6387224 .. 6390612

Gene Structure 3389 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1090400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1008 bp

ATGGCGGACGAGAGTGTGGCGGGGTCCGCTGATTTTCCGGTGAAGAGGCCCAGGGAGGAAGAAGAGAACGGCTCTGCAGCTGCTGCCTCAGCCGTCTCCATGGAGCCTGACGGTGGCAAGGAGCCGGATTCTGTCTCCGCTGTCATTCCCGGCTGGTTTTCTGAGATTAGCTCAATGTGGCCAGGAGAGGCACACTCCTTGAAGATAGAGAAGATTTTGTTTCAGGGAAAGTCCGACTATCAGAATGTGATGGTCTTCCAGTCAGCGACATATGGAAAGGTTCTTGTATTGGATGGGGTGATTCAGTTAACAGAAAGGGATGAATGTGCCTATCAAGAAATGATTACTCATCTCCCACTCTGCTCAATTCCAAATCCAAAGAAGGTTTTGGTTATTGGTGGAGGAGATGGTGGGGTACTGAGGGAAGTGGCTCGTTATTCTTCGGTTGAGAAGATTGATATATGTGAGATCGATAAGATGGTGATTGATGTGTCTAAACAATTTTTCCCTGAGGTAGCTATTGGATATGAGGACCCACGGGTCACACTTCATGTTGGTGATGGAGTTGCATTTTTGAAGGCTGTTCCCGCAGGAACTTATGATGCAGTAATAGTGGACTCTTCTGACCCAATAGGTCCTGCAAAAGAACTTTTTGAGAAGCCCTTTTTCCAGACAGTAGCTAATGCTCTTCGTCCGGGAGGAGTTGTGTGTACACAGGCAGAGAGTATATGGCTTCACATGCACATCATTGAGGATATTGTGGCAAACTGTCGCGAGATATTCAAAGGCTCTGTCAACTACGCATGGACTACAGTCCCTACATATCCAAGTGGGGTGATTGGTTTTATGCTTTGTTCTACTGAGGGACCTGCTGTGGATTTTAAGCACCCCGTGAATTCAATTGATGAGTCGAAACGGCCCTTGAGATTTTACAACTCTGAGATTCATACAGCTGCTTTCTGTCTACCATCTTTTGCAAAGAAGGTGATTGACGCAAAAGCAAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004766 GO:0005575 GO:0005623 GO:0005886 GO:0006576 GO:0006595 GO:0006596 GO:0006807 GO:0008150 GO:0008152 GO:0008216 GO:0008295 GO:0009058 GO:0009308 GO:0009309 GO:0009987 GO:0016020 GO:0016740 GO:0016765 GO:0034641 GO:0042401 GO:0044106 GO:0044237 GO:0044249 GO:0044271 GO:0044464 GO:0071704 GO:0071944 GO:0097164 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00270 ko00330 ko00410 ko00480 ko01100 map00270 map00330 map00410 map00480 map01100

Pfam Domains

Protein Families

Spermine_synt_N Spermine_synth

Protein Analysis

336

Amino Acids

36.44

Weight (kDa)

4.94

Isoelectric Point (pI)

45.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Spermine_synt_N	PF17284	51 - 105	3.3e-24	Spermidine synthase tetramerisation domain
Spermine_synth	PF01564	108 - 295	2.6e-80	Spermine/spermidine synthase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005047)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G23820 AT1G23820 AT1G70310
fragaria_vesca	FvH4_4g34680
malus_domestica	MD13G1090400.v1.1 MD14G1183600.v1.1 MD16G1091000.v1.1
prunus_persica	Prupe.1G255300_v2.0.a1 Prupe.5G176100_v2.0.a1
pyrus_communis	pycom13g07870 pycom16g07770
rosa_chinensis	RchiOBHm_Chr4g0444031 RchiOBHm_Chr7g0193881
rosa_laevigata	RLG00000004238 RLG00000005863
rosa_multiflora	Rmu_sc0015522.1_g000006
rosa_roxburghii	Rroxscaffold_3G00261760 Rroxscaffold_5G00384600
rosa_rugosa	Rorug04G0349100 Rorug07G0012500
rosa_samantha	Rh4AG409900 Rh4BG421000 Rh4CG435300 Rh4DG416200 Rh7AG139200 Rh7BG139600 Rh7CG143100 Rh7DG140700
rosa_wichuraiana	Rw4G035210 Rw7G011880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	539
Acc36I	ACCTGC	1 cut(s)	877
AccI	GTMKAC	1 cut(s)	964
AccII	CGCG	1 cut(s)	774
AciI	CCGC	5 cut(s)	5, 20, 27, 138, 588
AcoI	YGGCCR	1 cut(s)	179
AcsI	RAATTY	1 cut(s)	895
AdeI	CACNNNGTG	1 cut(s)	892
AfaI	GTAC	2 cut(s)	417, 712
AfiI	CCNNNNNNNGG	1 cut(s)	113
AgsI	TTSAA	4 cut(s)	202, 577, 784, 900
AjnI	CCWGG	2 cut(s)	50, 181
AluBI	AGCT	5 cut(s)	80, 171, 518, 680, 953
AluI	AGCT	5 cut(s)	80, 171, 518, 680, 953
Alw26I	GTCTC	2 cut(s)	100, 139
AlwNI	CAGNNNCTG	1 cut(s)	83
AoxI	GGCC	3 cut(s)	47, 179, 917
ApeKI	GCWGC	4 cut(s)	77, 80, 83, 953
ApoI	RAATTY	1 cut(s)	895
ArsI	GACNNNNNNTTYG	2 cut(s)	777, 809
AspS9I	GGNCC	6 cut(s)	24, 48, 532, 635, 866, 918
AsuC2I	CCSGG	2 cut(s)	150, 696
AsuHPI	GGTGA	6 cut(s)	52, 310, 493, 569, 847, 997
AvaII	GGWCC	4 cut(s)	24, 532, 635, 866
AxyI	CCTNAGG	1 cut(s)	510
BalI	TGGCCA	1 cut(s)	181
BbsI	GAAGAC	1 cut(s)	247
BbvCI	CCTCAGC	1 cut(s)	88
BbvI	GCAGC	4 cut(s)	67, 70, 89, 940
BccI	CCATC	6 cut(s)	244, 287, 401, 472, 554, 976
BceAI	ACGGC	3 cut(s)	77, 85, 932
BciT130I	CCWGG	2 cut(s)	52, 183
BcnI	CCSGG	2 cut(s)	150, 696
BcoDI	GTCTC	2 cut(s)	100, 139
BfmI	CTRYAG	2 cut(s)	75, 810
BfuAI	ACCTGC	1 cut(s)	877
BisI	GCNGC	4 cut(s)	78, 81, 84, 954
BlsI	GCNGC	4 cut(s)	79, 82, 85, 955
Bme1390I	CCNGG	4 cut(s)	52, 150, 183, 696
Bme18I	GGWCC	4 cut(s)	24, 532, 635, 866
BmgT120I	GGNCC	6 cut(s)	24, 48, 532, 635, 866, 918
BmiI	GGNNCC	5 cut(s)	25, 105, 123, 534, 867
BmrFI	CCNGG	4 cut(s)	52, 150, 183, 696
BmsI	GCATC	1 cut(s)	592
BoxI	GACNNNNGTC	1 cut(s)	812
BpiI	GAAGAC	1 cut(s)	247
BplI	GAGNNNNNCTC	6 cut(s)	56, 88, 155, 187, 179, 211
Bpu10I	CCTNAGC	1 cut(s)	88
BpuEI	CTTGAG	1 cut(s)	943
BpuMI	CCSGG	2 cut(s)	150, 696
Bsa29I	ATCGAT	1 cut(s)	471
BsaJI	CCNNGG	4 cut(s)	50, 51, 99, 536
BsaWI	WCCGGW	1 cut(s)	37
BsaXI	ACNNNNNCTCC	2 cut(s)	690, 720
Bsc4I	CCNNNNNNNGG	1 cut(s)	113
Bse1I	ACTGG	1 cut(s)	259
Bse21I	CCTNAGG	1 cut(s)	510
BseBI	CCWGG	2 cut(s)	52, 183
BseCI	ATCGAT	1 cut(s)	471
BseDI	CCNNGG	4 cut(s)	50, 51, 99, 536
BseGI	GGATG	2 cut(s)	298, 325
BseLI	CCNNNNNNNGG	1 cut(s)	113
BseMII	CTCAG	6 cut(s)	102, 153, 410, 501, 852, 930
BseNI	ACTGG	1 cut(s)	259
BseRI	GAGGAG	2 cut(s)	417, 714
BseXI	GCAGC	4 cut(s)	67, 70, 89, 940
Bsh1236I	CGCG	1 cut(s)	774
BshFI	GGCC	3 cut(s)	49, 181, 919
BshVI	ATCGAT	1 cut(s)	471
BsiSI	CCGG	4 cut(s)	38, 125, 150, 695
BslFI	GGGAC	2 cut(s)	800, 879
BslI	CCNNNNNNNGG	1 cut(s)	113
BsmAI	GTCTC	2 cut(s)	100, 139
BsmBI	CGTCTC	1 cut(s)	100
BsmFI	GGGAC	2 cut(s)	800, 879
BsnI	GGCC	3 cut(s)	49, 181, 919
Bsp1407I	TGTACA	1 cut(s)	710
Bsp143I	GATC	1 cut(s)	468
Bsp19I	CCATGG	1 cut(s)	99
Bsp68I	TCGCGA	1 cut(s)	774
BspACI	CCGC	5 cut(s)	5, 20, 27, 138, 588
BspANI	GGCC	3 cut(s)	49, 181, 919
BspCNI	CTCAG	6 cut(s)	101, 154, 411, 502, 853, 931
BspDI	ATCGAT	1 cut(s)	471
BspFNI	CGCG	1 cut(s)	774
BspLI	GGNNCC	5 cut(s)	25, 105, 123, 534, 867
BspMAI	CTGCAG	1 cut(s)	79
BspMI	ACCTGC	1 cut(s)	877
BspQI	GCTCTTC	1 cut(s)	693
BsrGI	TGTACA	1 cut(s)	710
BsrI	ACTGG	1 cut(s)	259
BssECI	CCNNGG	4 cut(s)	50, 51, 99, 536
BssMI	GATC	1 cut(s)	468
BssT1I	CCWWGG	1 cut(s)	99
Bst2UI	CCWGG	2 cut(s)	52, 183
Bst4CI	ACNGT	4 cut(s)	113, 676, 770, 814
Bst6I	CTCTTC	4 cut(s)	38, 57, 625, 693
BstAPI	GCANNNNNTGC	1 cut(s)	83
BstAUI	TGTACA	1 cut(s)	710
BstDEI	CTNAG	6 cut(s)	88, 162, 419, 510, 861, 939
BstDSI	CCRYGG	2 cut(s)	99, 536
BstF5I	GGATG	2 cut(s)	298, 325
BstFNI	CGCG	1 cut(s)	774
BstKTI	GATC	1 cut(s)	471
BstMAI	GTCTC	2 cut(s)	100, 139
BstMBI	GATC	1 cut(s)	468
BstMWI	GCNNNNNNNGC	5 cut(s)	26, 83, 89, 587, 739
BstNI	CCWGG	2 cut(s)	52, 183
BstNSI	RCATGY	1 cut(s)	742
BstPAI	GACNNNNGTC	1 cut(s)	812
BstSCI	CCNGG	4 cut(s)	50, 148, 181, 694
BstSFI	CTRYAG	2 cut(s)	75, 810
BstUI	CGCG	1 cut(s)	774
BstV1I	GCAGC	4 cut(s)	67, 70, 89, 940
BstV2I	GAAGAC	1 cut(s)	247
Bsu15I	ATCGAT	1 cut(s)	471
Bsu36I	CCTNAGG	1 cut(s)	510
BsuRI	GGCC	3 cut(s)	49, 181, 919
BsuTUI	ATCGAT	1 cut(s)	471
BtgI	CCRYGG	2 cut(s)	99, 536
BtsCI	GGATG	2 cut(s)	298, 325
BtuMI	TCGCGA	1 cut(s)	774
BveI	ACCTGC	1 cut(s)	877
CaiI	CAGNNNCTG	1 cut(s)	83
Cfr13I	GGNCC	6 cut(s)	24, 48, 532, 635, 866, 918
ClaI	ATCGAT	1 cut(s)	471
CseI	GACGC	1 cut(s)	1001
Csp6I	GTAC	2 cut(s)	416, 711
CviAII	CATG	4 cut(s)	100, 551, 739, 804
CviQI	GTAC	2 cut(s)	416, 711
DdeI	CTNAG	6 cut(s)	88, 162, 419, 510, 861, 939
DpnI	GATC	1 cut(s)	470
DpnII	GATC	1 cut(s)	468
DraIII	CACNNNGTG	1 cut(s)	892
DrdI	GACNNNNNNGTC	1 cut(s)	539
DseDI	GACNNNNNNGTC	1 cut(s)	539
EaeI	YGGCCR	1 cut(s)	179
Eam1104I	CTCTTC	4 cut(s)	38, 57, 625, 693
EarI	CTCTTC	4 cut(s)	38, 57, 625, 693
EciI	GGCGGA	1 cut(s)	20
Eco130I	CCWWGG	1 cut(s)	99
Eco47I	GGWCC	4 cut(s)	24, 532, 635, 866
Eco81I	CCTNAGG	1 cut(s)	510
EcoO109I	RGGNCCY	3 cut(s)	532, 635, 866
EcoRI	GAATTC	1 cut(s)	895
EcoRII	CCWGG	2 cut(s)	50, 181
EcoT14I	CCWWGG	1 cut(s)	99
ErhI	CCWWGG	1 cut(s)	99
Esp3I	CGTCTC	1 cut(s)	100
FaeI	CATG	4 cut(s)	103, 554, 742, 807
FaqI	GGGAC	2 cut(s)	800, 879
FatI	CATG	4 cut(s)	99, 550, 738, 803
FauI	CCCGC	2 cut(s)	13, 595
FauNDI	CATATG	1 cut(s)	271
FblI	GTMKAC	1 cut(s)	964
Fnu4HI	GCNGC	4 cut(s)	78, 81, 84, 954
FokI	GGATG	2 cut(s)	305, 332
Fsp4HI	GCNGC	4 cut(s)	78, 81, 84, 954
GluI	GCNGC	4 cut(s)	78, 81, 84, 954
HaeIII	GGCC	3 cut(s)	49, 181, 919
HapII	CCGG	4 cut(s)	38, 125, 150, 695
HgaI	GACGC	1 cut(s)	1001
Hin1II	CATG	4 cut(s)	103, 554, 742, 807
HincII	GTYRAC	2 cut(s)	309, 796
HindII	GTYRAC	2 cut(s)	309, 796
HinfI	GANTC	5 cut(s)	128, 301, 617, 908, 943
HpaI	GTTAAC	1 cut(s)	309
HpaII	CCGG	4 cut(s)	38, 125, 150, 695
HphI	GGTGA	6 cut(s)	52, 310, 493, 569, 847, 997
Hpy166II	GTNNAC	6 cut(s)	309, 616, 711, 713, 796, 965
Hpy188I	TCNGA	5 cut(s)	163, 235, 243, 625, 940
Hpy188III	TCNNGA	3 cut(s)	335, 670, 773
Hpy8I	GTNNAC	6 cut(s)	309, 616, 711, 713, 796, 965
HpyAV	CCTTC	3 cut(s)	376, 571, 976
HpyCH4III	ACNGT	4 cut(s)	113, 676, 770, 814
HpyCH4V	TGCA	6 cut(s)	77, 569, 605, 641, 742, 977
HpyF10VI	GCNNNNNNNGC	5 cut(s)	26, 83, 89, 587, 739
HpyF3I	CTNAG	6 cut(s)	88, 162, 419, 510, 861, 939
Hsp92II	CATG	4 cut(s)	103, 554, 742, 807
KspAI	GTTAAC	1 cut(s)	309
Kzo9I	GATC	1 cut(s)	468
LguI	GCTCTTC	1 cut(s)	693
LmnI	GCTCC	2 cut(s)	103, 121
Lsp1109I	GCAGC	4 cut(s)	67, 70, 89, 940
LweI	GCATC	1 cut(s)	592
MaeIII	GTNAC	1 cut(s)	541
MalI	GATC	1 cut(s)	470
MboI	GATC	1 cut(s)	468
MfeI	CAATTG	1 cut(s)	900
MlsI	TGGCCA	1 cut(s)	181
MluCI	AATT	4 cut(s)	366, 500, 895, 900
MluNI	TGGCCA	1 cut(s)	181
MlyI	GAGTC	2 cut(s)	611, 917
MmeI	TCCRAC	1 cut(s)	258
Mox20I	TGGCCA	1 cut(s)	181
MscI	TGGCCA	1 cut(s)	181
MseI	TTAA	2 cut(s)	308, 882
MslI	CAYNNNNRTG	1 cut(s)	555
Msp20I	TGGCCA	1 cut(s)	181
MspA1I	CMGCKG	4 cut(s)	29, 80, 140, 953
MspI	CCGG	4 cut(s)	38, 125, 150, 695
MspR9I	CCNGG	4 cut(s)	52, 150, 183, 696
MunI	CAATTG	1 cut(s)	900
MvaI	CCWGG	2 cut(s)	52, 183
MvnI	CGCG	1 cut(s)	774
MwoI	GCNNNNNNNGC	5 cut(s)	26, 83, 89, 587, 739
NciI	CCSGG	2 cut(s)	150, 696
NcoI	CCATGG	1 cut(s)	99
NdeI	CATATG	1 cut(s)	271
NdeII	GATC	1 cut(s)	468
NlaIII	CATG	4 cut(s)	103, 554, 742, 807
NlaIV	GGNNCC	5 cut(s)	25, 105, 123, 534, 867
NmuCI	GTSAC	1 cut(s)	541
NruI	TCGCGA	1 cut(s)	774
NspI	RCATGY	1 cut(s)	742
PasI	CCCWGGG	1 cut(s)	51
PciSI	GCTCTTC	1 cut(s)	693
PfeI	GAWTC	3 cut(s)	128, 301, 943
PfoI	TCCNGGA	1 cut(s)	694
PkrI	GCNGC	4 cut(s)	79, 82, 85, 955
PleI	GAGTC	2 cut(s)	611, 916
PpsI	GAGTC	2 cut(s)	611, 916
PpuMI	RGGWCCY	3 cut(s)	532, 635, 866
PshAI	GACNNNNGTC	1 cut(s)	812
Psp5II	RGGWCCY	3 cut(s)	532, 635, 866
Psp6I	CCWGG	2 cut(s)	50, 181
PspGI	CCWGG	2 cut(s)	50, 181
PspN4I	GGNNCC	5 cut(s)	25, 105, 123, 534, 867
PspPI	GGNCC	6 cut(s)	24, 48, 532, 635, 866, 918
PspPPI	RGGWCCY	3 cut(s)	532, 635, 866
PstI	CTGCAG	1 cut(s)	79
PstNI	CAGNNNCTG	1 cut(s)	83
PvuII	CAGCTG	2 cut(s)	80, 953
RruI	TCGCGA	1 cut(s)	774
RsaI	GTAC	2 cut(s)	417, 712
RsaNI	GTAC	2 cut(s)	416, 711
RseI	CAYNNNNRTG	1 cut(s)	555
SapI	GCTCTTC	1 cut(s)	693
SaqAI	TTAA	2 cut(s)	308, 882
SatI	GCNGC	4 cut(s)	78, 81, 84, 954
Sau3AI	GATC	1 cut(s)	468
Sau96I	GGNCC	6 cut(s)	24, 48, 532, 635, 866, 918
SchI	GAGTC	2 cut(s)	611, 917
ScrFI	CCNGG	4 cut(s)	52, 150, 183, 696
SfaNI	GCATC	1 cut(s)	592
SfcI	CTRYAG	2 cut(s)	75, 810
SinI	GGWCC	4 cut(s)	24, 532, 635, 866
SmiMI	CAYNNNNRTG	1 cut(s)	555
SmlI	CTYRAG	1 cut(s)	922
SmoI	CTYRAG	1 cut(s)	922
Sse9I	AATT	4 cut(s)	366, 500, 895, 900
SsiI	CCGC	5 cut(s)	5, 20, 27, 138, 588
StyD4I	CCNGG	4 cut(s)	50, 148, 181, 694
StyI	CCWWGG	1 cut(s)	99
TaaI	ACNGT	4 cut(s)	113, 676, 770, 814
TaqI	TCGA	2 cut(s)	471, 911
TasI	AATT	4 cut(s)	366, 500, 895, 900
TatI	WGTACW	1 cut(s)	710
TfiI	GAWTC	3 cut(s)	128, 301, 943
Tru1I	TTAA	2 cut(s)	308, 882
Tru9I	TTAA	2 cut(s)	308, 882
TseFI	GTSAC	1 cut(s)	541
TseI	GCWGC	4 cut(s)	77, 80, 83, 953
Tsp45I	GTSAC	1 cut(s)	541
TspDTI	ATGAA	3 cut(s)	336, 539, 935
VpaK11BI	GGWCC	4 cut(s)	24, 532, 635, 866
XapI	RAATTY	1 cut(s)	895
XceI	RCATGY	1 cut(s)	742
XmiI	GTMKAC	1 cut(s)	964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.