AT1G70750

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011757
Physical Location & Seq
Reverse (-)
26679872 .. 26683355
3484 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70750.1

Sequence Viewer

Length: 2250 bp
ATGGCGGCTAACAAATTCGCGACTCTGATTCATCGGAAAACAAACCGAATCACTTTAATCCTCGTATACGCTTTTCTCGAATGGTCACTCATTTTCTTCATTTTGCTCAACTCTCTCTTTTCTTATTTCATACTCAGATTCGCTGATTATTTCGGTCTTAAACGTCCTTGTCTCTTCTGCTCTAGACTCGATCGTTTCTTCGATGCTTCTGGTAAATCTCCTTCTCATCGAGATCTTCTCTGCGATGATCATGCTCTCCAATTACATTCAAAACCTGTTGAAGAATCTAATTGTGGTTTCGGAGAATTTCACAATGATTTGGTTCATCGTGGTTGTTGCGTAGAGAAGATAAGTTCGTCACTATGTGCTCCGATTGAGTCTGACTTTGGGAATTTAGATTATCCAATTGGAGATGAAGGTCAGATTTACAATGGTCTTAAGTTTCCTCGATCGATCTTCGTCTTTGAAGAAGAGAAAGTAGGATCTGTAAATTTGAATGATTCTCAGGAAGAAACAGAGGAGAAGAAAGTTCCCCAATCTCATGAGAAACTTGAAGATGATGATGTTGATGAGGAGTTTTCATGCTATGTATCAAGCTTCGATTGTAAGAACAAAGAAATTGCAACAGAGAAGGAAGAAGAAAACAGAGTGGATCTACCTATAGAGGTGGAAACTGCAGAATCAGCTCCGAAAAACCTCGAGTTCTATATTGATGAAGAAGACTGTCATTTGATTCCAGTTGAATTCTATAAACCGAGTGAAGAAGTTCGAGAGATTTCCGACATTAACGGAGATTTTATCCTCGATTTCGGCGTTGAGCATGATTTCACGGCGGCGGCGGAGACGGAGGAAATCTCCGACTTTGCTTCGCCGGGTGAATCGAAACCGGAGGATGCAGAGACGAATCTAGTTGCTTCGGAAATGGAAAACGACGACGAAGAAACAGACGCAGAGGTTTCTATAGGTACAGAGATTCCTGATCATGAGCAAATCGGAGATATTCCTTCTCACCAGCTCATTCCTCACCACGATGACGATGATCATGAGGAGGAAACGTTGGAGTTCAAAACAGTAACGATTGAAACCAAGATGCCAGTCTTAAACATCAACGAAGAGCGGATTTTAGAAGCTCAAGGCTCGATGGAAAGCTCGCATAGTAGTCTACATAACGCTATGTTTCACTTAGAGCAAAGAGTATCTGTTGATGGTATTGAATGTCCTGAAGGAGTACTCACTGTTGATAAGTTGAAGTTTGAGTTACAAGAAGAGAGAAAAGCACTTCACGCGTTATACGAGGAGCTGGAGGTAGAGAGGAATGCGTCTGCTGTTGCTGCCAGTGAAACAATGGCGATGATCAATAGGTTGCATGAGGAGAAAGCTGCGATGCAGATGGAAGCGTTGCAGTATCAGAGAATGATGGAGGAGCAAGCTGAGTTTGATCAAGAAGCTTTGCAGTTGTTGAATGAGCTTATGGTGAATAGAGAGAAGGAGAATGCTGAGCTTGAGAAGGAGCTAGAGGTGTATAGAAAGAGAATGGAGGAGTATGAAGCTAAAGAGAAAATGGGGATGTTGAGGAGGAGATTGAGAGATTCCTCTGTTGATTCGTATAGAAATAATGGCGATTCTGATGAGAATAGCAATGGAGAGTTACAGTTTAAGAACGTTGAAGGGGTTACGGATTGGAAATATAGAGAGAATGAGATGGAGAATACGCCGGTGGATGTTGTACTTCGTCTTGATGAGTGTTTAGATGATTATGATGGAGAGAGGCTTTCGATTCTTGGGAGATTGAAGTTTCTTGAAGAGAAACTCACAGATCTTAATAACGAAGAGGACGACGAGGAGGAGGCTAAAACGTTTGAGAGTAATGGTAGCATCAATGGAAATGAGCATATTCATGGCAAAGAAACAAACGGGAAGCACAGAGTTATCAAGTCAAAGAGATTACTTCCCCTGTTTGATGCGGTCGATGGAGAGATGGAAAACGGGTTAAGTAACGGAAACCATCACGAAAACGGGTTTGATGATTCGGAGAAGGGTGAGAATGTGACGATAGAAGAAGAAGTGGATGAGCTTTACGAGAGGTTAGAAGCTCTAGAGGCAGATAGAGAGTTCTTAAGACATTGTGTTGGTTCATTGAAAAAAGGAGACAAAGGTGTACATCTCCTCCATGAGATTCTGCAACATCTTCGTGATCTAAGGAATATCGATCTTACTCGCGTCAGAGAAAACGGAGACATGAGTTTATGA

Protein Analysis

749

Amino Acids

86.17

Weight (kDa)

4.5

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 415 - 505 8.7e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1117
AccI GTMKAC 2 cut(s) 66, 1162
AccII CGCG 3 cut(s) 20, 1286, 2220
AciI CCGC 6 cut(s) 5, 833, 836, 839, 1117, 1964
AclI AACGTT 3 cut(s) 1055, 1662, 1856
AclWI GGATC 2 cut(s) 490, 660
AcsI RAATTY 5 cut(s) 14, 305, 391, 490, 743
AcuI CTGAAG 1 cut(s) 1242
AdeI CACNNNGTG 1 cut(s) 365
AfaI GTAC 4 cut(s) 967, 1230, 1728, 2160
AflII CTTAAG 2 cut(s) 437, 2116
AflIII ACRYGT 1 cut(s) 1284
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 5 cut(s) 176, 836, 893, 2142, 2229
AlwI GGATC 2 cut(s) 490, 660
Ama87I CYCGRG 1 cut(s) 698
ApeKI GCWGC 2 cut(s) 1331, 1379
ApoI RAATTY 5 cut(s) 14, 305, 391, 490, 743
Asp700I GAANNNNTTC 1 cut(s) 765
AsuC2I CCSGG 1 cut(s) 873
AsuHPI GGTGA 5 cut(s) 887, 1001, 1016, 1486, 2051
AvaI CYCGRG 1 cut(s) 698
BarI GAAGNNNNNNTAC 2 cut(s) 205, 237
BbsI GAAGAC 1 cut(s) 726
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 2 cut(s) 1318, 1366
BccI CCATC 9 cut(s) 1135, 1199, 1384, 1411, 1696, 1754, 1964, 1972, 2013
BceAI ACGGC 1 cut(s) 846
BcgI CGANNNNNNTGC 4 cut(s) 233, 267, 2171, 2205
BclI TGATCA 5 cut(s) 247, 979, 1039, 1353, 1438
BcnI CCSGG 1 cut(s) 873
BcoDI GTCTC 5 cut(s) 176, 836, 893, 2142, 2229
BfaI CTAG 4 cut(s) 183, 908, 1514, 2096
BfmI CTRYAG 3 cut(s) 660, 675, 960
BfrI CTTAAG 2 cut(s) 437, 2116
BglII AGATCT 2 cut(s) 232, 1816
BisI GCNGC 5 cut(s) 6, 834, 837, 1332, 1380
BlpI GCTNAGC 1 cut(s) 1497
BlsI GCNGC 5 cut(s) 7, 835, 838, 1333, 1381
BmcAI AGTACT 1 cut(s) 1230
Bme1390I CCNGG 1 cut(s) 873
BmeT110I CYCGRG 1 cut(s) 698
BmrFI CCNGG 1 cut(s) 873
BmsI GCATC 6 cut(s) 193, 883, 1080, 1374, 1884, 1951
BpiI GAAGAC 1 cut(s) 726
BplI GAGNNNNNCTC 4 cut(s) 222, 254, 839, 871
BpmI CTGGAG 1 cut(s) 1322
Bpu1102I GCTNAGC 1 cut(s) 1497
BpuEI CTTGAG 2 cut(s) 1116, 1523
BpuMI CCSGG 1 cut(s) 873
Bsa29I ATCGAT 2 cut(s) 452, 2208
BsaWI WCCGGW 1 cut(s) 886
BsaXI ACNNNNNCTCC 4 cut(s) 1040, 1070, 2151, 2181
Bse118I RCCGGY 1 cut(s) 1714
Bse1I ACTGG 3 cut(s) 737, 1094, 1335
Bse3DI GCAATG 1 cut(s) 1645
BseCI ATCGAT 2 cut(s) 452, 2208
BseGI GGATG 4 cut(s) 898, 1572, 1726, 2074
BseMI GCAATG 1 cut(s) 1645
BseMII CTCAG 4 cut(s) 148, 518, 1422, 1488
BseNI ACTGG 3 cut(s) 737, 1094, 1335
BseXI GCAGC 2 cut(s) 1318, 1366
Bsh1236I CGCG 3 cut(s) 20, 1286, 2220
Bsh1285I CGRYCG 3 cut(s) 193, 452, 1968
BshVI ATCGAT 2 cut(s) 452, 2208
BsiEI CGRYCG 3 cut(s) 193, 452, 1968
BsiHKAI GWGCWC 1 cut(s) 370
BsiHKCI CYCGRG 1 cut(s) 698
BsiSI CCGG 3 cut(s) 872, 887, 1715
BsmAI GTCTC 5 cut(s) 176, 836, 893, 2142, 2229
BsmBI CGTCTC 2 cut(s) 836, 893
BsmI GAATGC 2 cut(s) 1321, 1498
BsoBI CYCGRG 1 cut(s) 698
Bsp1286I GDGCHC 1 cut(s) 370
Bsp1407I TGTACA 1 cut(s) 2158
Bsp1720I GCTNAGC 1 cut(s) 1497
Bsp68I TCGCGA 1 cut(s) 20
BspACI CCGC 6 cut(s) 5, 833, 836, 839, 1117, 1964
BspCNI CTCAG 4 cut(s) 147, 517, 1423, 1489
BspDI ATCGAT 2 cut(s) 452, 2208
BspFNI CGCG 3 cut(s) 20, 1286, 2220
BspHI TCATGA 3 cut(s) 541, 982, 1042
BspMAI CTGCAG 1 cut(s) 679
BspPI GGATC 2 cut(s) 490, 660
BspQI GCTCTTC 1 cut(s) 1107
BspTI CTTAAG 2 cut(s) 437, 2116
BsrBI CCGCTC 1 cut(s) 1117
BsrDI GCAATG 1 cut(s) 1645
BsrFI RCCGGY 1 cut(s) 1714
BsrGI TGTACA 1 cut(s) 2158
BsrI ACTGG 3 cut(s) 737, 1094, 1335
BssAI RCCGGY 1 cut(s) 1714
BssNAI GTATAC 1 cut(s) 67
Bst1107I GTATAC 1 cut(s) 67
Bst4CI ACNGT 4 cut(s) 725, 1072, 1237, 1653
Bst6I CTCTTC 6 cut(s) 179, 465, 1107, 1260, 1797, 1824
BstAFI CTTAAG 2 cut(s) 437, 2116
BstAUI TGTACA 1 cut(s) 2158
BstC8I GCNNGC 2 cut(s) 1151, 1428
BstDEI CTNAG 6 cut(s) 134, 504, 1183, 1431, 1497, 2198
BstF5I GGATG 4 cut(s) 898, 1572, 1726, 2074
BstFNI CGCG 3 cut(s) 20, 1286, 2220
BstMAI GTCTC 5 cut(s) 176, 836, 893, 2142, 2229
BstMCI CGRYCG 3 cut(s) 193, 452, 1968
BstMWI GCNNNNNNNGC 4 cut(s) 683, 1283, 1331, 2099
BstSCI CCNGG 1 cut(s) 871
BstSFI CTRYAG 3 cut(s) 660, 675, 960
BstUI CGCG 3 cut(s) 20, 1286, 2220
BstV1I GCAGC 2 cut(s) 1318, 1366
BstV2I GAAGAC 1 cut(s) 726
BstX2I RGATCY 4 cut(s) 232, 482, 652, 1816
BstYI RGATCY 4 cut(s) 232, 482, 652, 1816
BstZ17I GTATAC 1 cut(s) 67
Bsu15I ATCGAT 2 cut(s) 452, 2208
BsuTUI ATCGAT 2 cut(s) 452, 2208
BtgZI GCGATG 3 cut(s) 258, 1364, 1397
BtsCI GGATG 4 cut(s) 898, 1572, 1726, 2074
BtsIMutI CAGTG 2 cut(s) 1233, 1342
BtuMI TCGCGA 1 cut(s) 20
Cac8I GCNNGC 2 cut(s) 1151, 1428
CciI TCATGA 3 cut(s) 541, 982, 1042
Cfr10I RCCGGY 1 cut(s) 1714
ClaI ATCGAT 2 cut(s) 452, 2208
CseI GACGC 3 cut(s) 956, 1308, 2209
Csp6I GTAC 4 cut(s) 966, 1229, 1727, 2159
CviQI GTAC 4 cut(s) 966, 1229, 1727, 2159
DdeI CTNAG 6 cut(s) 134, 504, 1183, 1431, 1497, 2198
DraIII CACNNNGTG 1 cut(s) 365
Eam1104I CTCTTC 6 cut(s) 179, 465, 1107, 1260, 1797, 1824
EarI CTCTTC 6 cut(s) 179, 465, 1107, 1260, 1797, 1824
EciI GGCGGA 1 cut(s) 854
Eco57I CTGAAG 1 cut(s) 1242
Eco88I CYCGRG 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 743
Esp3I CGTCTC 2 cut(s) 836, 893
FbaI TGATCA 5 cut(s) 247, 979, 1039, 1353, 1438
FblI GTMKAC 2 cut(s) 66, 1162
Fnu4HI GCNGC 5 cut(s) 6, 834, 837, 1332, 1380
FokI GGATG 4 cut(s) 905, 1579, 1733, 2081
Fsp4HI GCNGC 5 cut(s) 6, 834, 837, 1332, 1380
FspBI CTAG 4 cut(s) 183, 908, 1514, 2096
GluI GCNGC 5 cut(s) 6, 834, 837, 1332, 1380
GsuI CTGGAG 1 cut(s) 1322
HapII CCGG 3 cut(s) 872, 887, 1715
HgaI GACGC 3 cut(s) 956, 1308, 2209
HindIII AAGCTT 2 cut(s) 595, 1446
HpaII CCGG 3 cut(s) 872, 887, 1715
HphI GGTGA 5 cut(s) 887, 1001, 1016, 1486, 2051
Hpy166II GTNNAC 3 cut(s) 67, 1163, 2159
Hpy8I GTNNAC 3 cut(s) 67, 1163, 2159
Hpy99I CGWCG 3 cut(s) 935, 938, 1841
HpyAV CCTTC 9 cut(s) 231, 410, 625, 1014, 1217, 1480, 1501, 1661, 2029
HpyCH4III ACNGT 4 cut(s) 725, 1072, 1237, 1653
HpyCH4IV ACGT 4 cut(s) 163, 1055, 1662, 1856
HpyCH4V TGCA 8 cut(s) 623, 677, 896, 1366, 1387, 1402, 1453, 2182
HpyF10VI GCNNNNNNNGC 4 cut(s) 683, 1283, 1331, 2099
HpyF3I CTNAG 6 cut(s) 134, 504, 1183, 1431, 1497, 2198
HpySE526I ACGT 4 cut(s) 163, 1055, 1662, 1856
Ksp22I TGATCA 5 cut(s) 247, 979, 1039, 1353, 1438
LguI GCTCTTC 1 cut(s) 1107
LmnI GCTCC 5 cut(s) 373, 691, 1297, 1423, 1510
Lsp1109I GCAGC 2 cut(s) 1318, 1366
LweI GCATC 6 cut(s) 193, 883, 1080, 1374, 1884, 1951
MaeI CTAG 4 cut(s) 183, 908, 1514, 2096
MaeII ACGT 4 cut(s) 163, 1055, 1662, 1856
MaeIII GTNAC 8 cut(s) 84, 357, 1072, 1257, 1647, 1672, 1994, 2047
MbiI CCGCTC 1 cut(s) 1117
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 4 cut(s) 232, 482, 652, 1816
MhlI GDGCHC 1 cut(s) 370
MluCI AATT 9 cut(s) 14, 260, 289, 305, 391, 405, 490, 618, 743
MluI ACGCGT 1 cut(s) 1284
MlyI GAGTC 3 cut(s) 16, 180, 386
MmeI TCCRAC 3 cut(s) 804, 882, 1038
MroXI GAANNNNTTC 1 cut(s) 765
MseI TTAA 9 cut(s) 56, 159, 438, 786, 1100, 1656, 1821, 1991, 2117
MslI CAYNNNNRTG 3 cut(s) 1029, 1896, 2190
MspCI CTTAAG 2 cut(s) 437, 2116
MspI CCGG 3 cut(s) 872, 887, 1715
MspR9I CCNGG 1 cut(s) 873
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 2 cut(s) 1321, 1498
MvnI CGCG 3 cut(s) 20, 1286, 2220
MwoI GCNNNNNNNGC 4 cut(s) 683, 1283, 1331, 2099
NciI CCSGG 1 cut(s) 873
NmuCI GTSAC 3 cut(s) 84, 357, 2047
NruI TCGCGA 1 cut(s) 20
PaeR7I CTCGAG 1 cut(s) 698
PagI TCATGA 3 cut(s) 541, 982, 1042
PciSI GCTCTTC 1 cut(s) 1107
PcsI WCGNNNNNNNCGW 4 cut(s) 75, 810, 1290, 1833
PctI GAATGC 2 cut(s) 1321, 1498
PdmI GAANNNNTTC 1 cut(s) 765
PkrI GCNGC 5 cut(s) 7, 835, 838, 1333, 1381
Ple19I CGATCG 2 cut(s) 193, 452
PleI GAGTC 3 cut(s) 16, 180, 385
PpsI GAGTC 3 cut(s) 16, 180, 385
Psp1406I AACGTT 3 cut(s) 1055, 1662, 1856
PspXI VCTCGAGB 1 cut(s) 698
PstI CTGCAG 1 cut(s) 679
PsuI RGATCY 4 cut(s) 232, 482, 652, 1816
PvuI CGATCG 2 cut(s) 193, 452
RruI TCGCGA 1 cut(s) 20
RsaI GTAC 4 cut(s) 967, 1230, 1728, 2160
RsaNI GTAC 4 cut(s) 966, 1229, 1727, 2159
RseI CAYNNNNRTG 3 cut(s) 1029, 1896, 2190
SapI GCTCTTC 1 cut(s) 1107
SaqAI TTAA 9 cut(s) 56, 159, 438, 786, 1100, 1656, 1821, 1991, 2117
SatI GCNGC 5 cut(s) 6, 834, 837, 1332, 1380
ScaI AGTACT 1 cut(s) 1230
SchI GAGTC 3 cut(s) 16, 180, 386
ScrFI CCNGG 1 cut(s) 873
SduI GDGCHC 1 cut(s) 370
SfaNI GCATC 6 cut(s) 193, 883, 1080, 1374, 1884, 1951
SfcI CTRYAG 3 cut(s) 660, 675, 960
Sfr274I CTCGAG 1 cut(s) 698
SgrAI CRCCGGYG 1 cut(s) 1714
SlaI CTCGAG 1 cut(s) 698
SmiMI CAYNNNNRTG 3 cut(s) 1029, 1896, 2190
SmlI CTYRAG 5 cut(s) 437, 698, 1131, 1502, 2116
SmoI CTYRAG 5 cut(s) 437, 698, 1131, 1502, 2116
Sse9I AATT 9 cut(s) 14, 260, 289, 305, 391, 405, 490, 618, 743
SsiI CCGC 6 cut(s) 5, 833, 836, 839, 1117, 1964
SspMI CTAG 4 cut(s) 183, 908, 1514, 2096
StyD4I CCNGG 1 cut(s) 871
TaaI ACNGT 4 cut(s) 725, 1072, 1237, 1653
TaiI ACGT 4 cut(s) 166, 1058, 1665, 1859
TaqII GACCGA 1 cut(s) 143
TasI AATT 9 cut(s) 14, 260, 289, 305, 391, 405, 490, 618, 743
TatI WGTACW 3 cut(s) 1228, 1726, 2158
TauI GCSGC 3 cut(s) 8, 836, 839
Tru1I TTAA 9 cut(s) 56, 159, 438, 786, 1100, 1656, 1821, 1991, 2117
Tru9I TTAA 9 cut(s) 56, 159, 438, 786, 1100, 1656, 1821, 1991, 2117
TscAI CASTG 2 cut(s) 1240, 1342
TseFI GTSAC 3 cut(s) 84, 357, 2047
TseI GCWGC 2 cut(s) 1331, 1379
Tsp45I GTSAC 3 cut(s) 84, 357, 2047
TspGWI ACGGA 5 cut(s) 804, 860, 1691, 2013, 2247
TspRI CASTG 2 cut(s) 1240, 1342
Vha464I CTTAAG 2 cut(s) 437, 2116
XapI RAATTY 5 cut(s) 14, 305, 391, 490, 743
XbaI TCTAGA 2 cut(s) 182, 2095
XcmI CCANNNNNNNNNTGG 1 cut(s) 1342
XhoI CTCGAG 1 cut(s) 698
XmiI GTMKAC 2 cut(s) 66, 1162
XmnI GAANNNNTTC 1 cut(s) 765
XspI CTAG 4 cut(s) 183, 908, 1514, 2096
ZrmI AGTACT 1 cut(s) 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.