Rw4G034740

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011757
Physical Location & Seq
Reverse (-)
61725255 .. 61729056
3802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034740.1

Sequence Viewer

Length: 2865 bp
ATGGCTGCCAACAAGTTTGCCACCATGTTACACAGAAACACCAACAAACTCACTCTGATTCTCATCTACGCAATCCTCGAATGGGTTCTGATCCTCCTCCTCCTTCTCAACTCCCTCTTCTCTTATCTGATCGTCAAGTTCGCCGATTACTTTGGCCTCAAACGACCCTGCCTCTGGTGTTCTAGGCTCGATCACATCTTCGAGCCCGGAAACAAGAGCCACAGTTCTTACAGAGATCTTGTGTGTGAAGAACACGCCAATGAAATAGCCAAACTTGGGTTTTGCTCGAATCACCAGAAACTGGCTGAGTCTCAAGATATGTGTGAGGATTGCTCATCGCTGGAATATTCTCAAGAATGGTCTAACAAGTATGCTTTCTTTCCGTGGATGAAGCAAATTGGGATGATTCAAGGCGGCGATGATGATAAGGAGATTAACGATAAGGAATGTTCTTGCTGCGGTGTGAGCTTGAGCAACAAGCTGTACCCTCCTTGTATTCTGCTCAAGCCTTCTTGGGGGGTTTTGGATTACGCTCAGAAACCCATTTTGAGTTCAGATGTAGGGGTTGATGCTCAGACTGAGGAAGGTGATCAGTCGGATCAAAGTGGATCGGATTATGTGGCAGATCAAAATGGACATGATGAGGGTGTTTCTGTTGTTGTTGTTCAAGGAAATAGGGGAGAGAATTCGGTTTCGGTTTCGGTTTCGGATGTTGATGGATGTTGTGGACAAAGAGACTACGATGCAGAGGAGAAGAATAATTCAGCATGTTGTTCTGTCTGTGACTATGTCTGCAAGGAAACGGAAACTGAGGCCAATCAAATTGAAGACGAGGCTAATATGAATATCTCCATGGACGATGGTGATGATGGTCGAGCTCAAATTCAGGCCAGTTGTGATGAGAATAAGTCTCGTGGAAGTCTGCATCAGCATCTGGAGTTCTACATTGATCAAGATGATTGTCGGTTGGTTCCGGTTGAATTGGTTGATTCTCCTGCCACCATCGAGAGGCAAAGTCACCAGAATTATAAGGTGGAGGAAGACCGAGCACATAGTGATACTCAAGATGTTATTCTGGATTTCGACATGCATTTCGAGACACAAGCCAAACCGGTTGTTGAGTTGTGGCACAGTTCAGATGACATTGTGGAATTACTTTCATCCCATGAGAGTAAAGAGGAGAGCAAAGTTTCAGTTCTTGACTCAAAGGATTTGGATTTCGATATGCATTTCAAGACACAAGCCAAACCGGTTGTTGAGTGTTGGCAGAGTTCAGATGAGATTGTGGAATTACTTTCATCCCATGAGATTAAAGAGGAGAGCAAAGATGATGACATCCATATTCGGAACGATGAGATTGATACAGAGGTCTCAATAGGGACTGATATTCCTGATCATGATATATTCAATGAGGATATTCAATCAGCTCATGAACTTCTACATTCTTATCCCAGTGTGCAAGAAGATCCTTCTACAAGTTCTCCCAATGCTGAAATTGATGATAATCATGGTGCTAAGCAAGAAGAAGAGTTTCTAGAATTCAAGACCTTATCAGTTGAAACAAGTGAGAAAGAACAAAATAGTTATTTCTCATTAGGTTCAGAGCTTAATGAGATTGAGGAAGAGAAGGTTCTTCCTGATACACCAACTTCTATAGACAATTTCCATCAGTTGTATAATAGAATGCTACTAACTGAGAGAAGGGAATCAGGAACAGAAGCATCATTGGATGGAAGTGTAGTGAGCGACATTGAATGTGGTGATGGAGTCCTGACTATGGAGAAGCTGAAATCGGTGTTGAGAGCTGAACGGAAAGCTTTAAATAGAGTATATGCAGAACTTGAAGAAGAGAGGAGTGCTTCTGCAATAGCAGCCAACCAGACAATGGCAATGATAAATAGGCTACAAGAAGAAAAGGCAGCAATGCAGATGGAAGCTTTGCAGTACCAAAGGATGATGGATGAGCAGTCTGAGTACGACCAGGAAGCTATGCAGCTCTTAAACGAGCTCATGGTAAAGAGGGAAAAGGAAAAGCAAGAACTTGAAAAGGAGCTAGAAATATGTCGAAAGAAGGTCCAAGATTATGAGGCCAAAGAGAAAATGATGATTTTGAGGAGAATGAAAGAGGGCTGCACAAGAAGTAGGACTTCATCTGGTCCTTGTAGCAATGCTGAGGATAGTGATGGACTTTCTATTGATTTGAATCATGAAGTGAAGGAAGAAGATAACCTCTCGGGACATGAAGAGAGTAGCAATCAAAACACACCAAGGGATGCGGTGTTATATTTAGAAGAATCTTTGGCTAGCTTTGAGGAAGAGAAGTTATCCATTCTAGACCAACTCAAGGAATTGGAAGAGAAGCTTTTGACCTTGAATGACGAAGAGGAAGAAGAACATTACGAGGATATAAAACCAATTGATCATTTCTTTTCAGAGAATGGGAATGGCTACCATGAGAATGGTGATGTTGGCGGTGAAGAAAATGGCATTGCAAATGGCCACTCCAAAGAAACGAATGGAACACATCATCAAGATAGAAAAATTATGGGATCAATTAAGGCAAAGAGACTTCTTCCACTTTTCAATGAAGCTGAAGATGAAGACGAGGAGTTGAATGGAGATGTAGAAGGTTATGATTCTGTTGCATCACAAGATCACAAGAAACTCGCTATTGAAGAGGAGGTTGCGCATGTCTATGAGAGGCTACAAGCACTTGAAGCAGATAGAGAGTTTCTAAAGCACTGTATTAGCTCCTTAAGGAAAGGTGACAAGGGATTAGATCTTCTCCAAGAAATTTTGCAACATCTTCGTGACCTGAGAAGTGTGGAAACCCGTCTAAGGAATTCGGGAGAGGGTATCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

954

Amino Acids

108.37

Weight (kDa)

4.49

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 595 - 685 9.6e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1029
Acc16I TGCGCA 1 cut(s) 2688
AccB7I CCANNNNNTGG 2 cut(s) 301, 1885
AciI CCGC 4 cut(s) 414, 459, 2276, 2472
AclWI GGATC 5 cut(s) 85, 606, 616, 1460, 2557
AcoI YGGCCR 1 cut(s) 2497
AcsI RAATTY 5 cut(s) 685, 882, 1538, 2793, 2842
AcuI CTGAAG 1 cut(s) 2613
AdeI CACNNNGTG 1 cut(s) 1055
AfaI GTAC 3 cut(s) 485, 1946, 1976
AflII CTTAAG 1 cut(s) 2755
AgeI ACCGGT 2 cut(s) 1111, 1249
AjnI CCWGG 1 cut(s) 1980
Alw21I GWGCWC 3 cut(s) 880, 1051, 2010
Alw26I GTCTC 6 cut(s) 315, 729, 915, 1091, 1375, 2560
AlwI GGATC 5 cut(s) 85, 606, 616, 1460, 2557
AlwNI CAGNNNCTG 2 cut(s) 301, 934
Ama87I CYCGRG 1 cut(s) 2233
AoxI GGCC 5 cut(s) 154, 813, 888, 2088, 2497
ApeKI GCWGC 6 cut(s) 5, 456, 1871, 1919, 1993, 2130
ApoI RAATTY 5 cut(s) 685, 882, 1538, 2793, 2842
ArsI GACNNNNNNTTYG 2 cut(s) 1076, 1108
AsiGI ACCGGT 2 cut(s) 1111, 1249
Asp700I GAANNNNTTC 2 cut(s) 84, 1530
AspLEI GCGC 1 cut(s) 2689
AspS9I GGNCC 2 cut(s) 2074, 2156
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 8 cut(s) 284, 599, 875, 1010, 1772, 2474, 2486, 2777
AsuNHI GCTAGC 1 cut(s) 2303
AvaI CYCGRG 1 cut(s) 2233
AvaII GGWCC 2 cut(s) 2074, 2156
BaeI ACNNNNGTAYC 2 cut(s) 1353, 1386
BalI TGGCCA 1 cut(s) 2499
BanII GRGCYC 3 cut(s) 207, 880, 2010
BauI CACGAG 1 cut(s) 912
BbsI GAAGAC 3 cut(s) 834, 1047, 2607
Bbv12I GWGCWC 3 cut(s) 880, 1051, 2010
BbvCI CCTCAGC 1 cut(s) 2172
BbvI GCAGC 5 cut(s) 443, 1883, 1931, 2005, 2117
BcgI CGANNNNNNTGC 2 cut(s) 2789, 2823
BciT130I CCWGG 1 cut(s) 1982
BclI TGATCA 4 cut(s) 589, 949, 1393, 2419
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 6 cut(s) 315, 729, 915, 1091, 1375, 2560
BfaI CTAG 5 cut(s) 183, 1535, 2054, 2304, 2333
BfmI CTRYAG 1 cut(s) 1653
BfrI CTTAAG 1 cut(s) 2755
BglII AGATCT 2 cut(s) 235, 2779
BisI GCNGC 7 cut(s) 6, 415, 457, 1872, 1920, 1994, 2131
BlpI GCTNAGC 1 cut(s) 1515
BlsI GCNGC 7 cut(s) 7, 416, 458, 1873, 1921, 1995, 2132
Bme1390I CCNGG 2 cut(s) 207, 1982
Bme18I GGWCC 2 cut(s) 2074, 2156
BmeT110I CYCGRG 1 cut(s) 2233
BmgT120I GGNCC 2 cut(s) 2074, 2156
BmiI GGNNCC 1 cut(s) 972
BmrFI CCNGG 2 cut(s) 207, 1982
BmrI ACTGGG 1 cut(s) 1446
BmsI GCATC 7 cut(s) 559, 733, 934, 940, 1730, 2263, 2654
BmtI GCTAGC 1 cut(s) 2307
BmuI ACTGGG 1 cut(s) 1446
BpiI GAAGAC 3 cut(s) 834, 1047, 2607
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 956
Bpu10I CCTNAGC 1 cut(s) 2172
Bpu1102I GCTNAGC 1 cut(s) 1515
BpuEI CTTGAG 6 cut(s) 297, 336, 488, 490, 1047, 2327
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 3 cut(s) 62, 1503, 2202
BsaI GGTCTC 1 cut(s) 1375
BsaJI CCNNGG 3 cut(s) 383, 852, 2267
BsaWI WCCGGW 3 cut(s) 973, 1111, 1249
BsaXI ACNNNNNCTCC 2 cut(s) 1465, 1495
Bse118I RCCGGY 2 cut(s) 1111, 1249
Bse1I ACTGG 3 cut(s) 306, 891, 1452
Bse3DI GCAATG 4 cut(s) 1896, 1929, 2173, 2487
Bse8I GATNNNNATC 3 cut(s) 62, 1503, 2202
BseBI CCWGG 1 cut(s) 1982
BseDI CCNNGG 3 cut(s) 383, 852, 2267
BseJI GATNNNNATC 3 cut(s) 62, 1503, 2202
BseMI GCAATG 4 cut(s) 1896, 1929, 2173, 2487
BseMII CTCAG 9 cut(s) 297, 548, 570, 587, 801, 1686, 1962, 2163, 2807
BseNI ACTGG 3 cut(s) 306, 891, 1452
BseRI GAGGAG 9 cut(s) 86, 89, 764, 1193, 1331, 1867, 2128, 2621, 2693
BseXI GCAGC 5 cut(s) 443, 1883, 1931, 2005, 2117
BsgI GTGCAG 1 cut(s) 2116
BshFI GGCC 5 cut(s) 156, 815, 890, 2090, 2499
BshTI ACCGGT 2 cut(s) 1111, 1249
BsiHKAI GWGCWC 3 cut(s) 880, 1051, 2010
BsiHKCI CYCGRG 1 cut(s) 2233
BsiSI CCGG 4 cut(s) 207, 974, 1112, 1250
BslFI GGGAC 2 cut(s) 1393, 2250
BsmAI GTCTC 6 cut(s) 315, 729, 915, 1091, 1375, 2560
BsmFI GGGAC 2 cut(s) 1393, 2250
BsmI GAATGC 1 cut(s) 1689
BsnI GGCC 5 cut(s) 156, 815, 890, 2090, 2499
Bso31I GGTCTC 1 cut(s) 1375
BsoBI CYCGRG 1 cut(s) 2233
Bsp1286I GDGCHC 4 cut(s) 207, 880, 1051, 2010
Bsp1720I GCTNAGC 1 cut(s) 1515
Bsp19I CCATGG 1 cut(s) 852
BspACI CCGC 4 cut(s) 414, 459, 2276, 2472
BspANI GGCC 5 cut(s) 156, 815, 890, 2090, 2499
BspCNI CTCAG 9 cut(s) 298, 547, 571, 586, 802, 1687, 1963, 2164, 2808
BspHI TCATGA 3 cut(s) 1396, 1429, 2206
BspLI GGNNCC 1 cut(s) 972
BspOI GCTAGC 1 cut(s) 2307
BspPI GGATC 5 cut(s) 85, 606, 616, 1460, 2557
BspTI CTTAAG 1 cut(s) 2755
BspTNI GGTCTC 1 cut(s) 1375
BsrDI GCAATG 4 cut(s) 1896, 1929, 2173, 2487
BsrFI RCCGGY 2 cut(s) 1111, 1249
BsrI ACTGG 3 cut(s) 306, 891, 1452
BssAI RCCGGY 2 cut(s) 1111, 1249
BssECI CCNNGG 3 cut(s) 383, 852, 2267
BssSI CACGAG 1 cut(s) 912
BssT1I CCWWGG 2 cut(s) 852, 2267
Bst2BI CACGAG 1 cut(s) 912
Bst2UI CCWGG 1 cut(s) 1982
Bst4CI ACNGT 3 cut(s) 224, 1133, 2744
Bst6I CTCTTC 9 cut(s) 122, 1521, 1617, 1842, 2238, 2310, 2349, 2376, 2670
BstAFI CTTAAG 1 cut(s) 2755
BstC8I GCNNGC 1 cut(s) 2305
BstDSI CCRYGG 2 cut(s) 383, 852
BstHHI GCGC 1 cut(s) 2689
BstMAI GTCTC 6 cut(s) 315, 729, 915, 1091, 1375, 2560
BstMWI GCNNNNNNNGC 3 cut(s) 465, 1871, 2717
BstNI CCWGG 1 cut(s) 1982
BstNSI RCATGY 3 cut(s) 771, 1090, 2693
BstSCI CCNGG 2 cut(s) 205, 1980
BstSFI CTRYAG 1 cut(s) 1653
BstV1I GCAGC 5 cut(s) 443, 1883, 1931, 2005, 2117
BstV2I GAAGAC 3 cut(s) 834, 1047, 2607
BstX2I RGATCY 3 cut(s) 235, 1465, 2779
BstXI CCANNNNNNTGG 1 cut(s) 2459
BstYI RGATCY 3 cut(s) 235, 1465, 2779
BsuRI GGCC 5 cut(s) 156, 815, 890, 2090, 2499
BtgI CCRYGG 2 cut(s) 383, 852
BtgZI GCGATG 2 cut(s) 321, 432
BtsIMutI CAGTG 2 cut(s) 1459, 2740
Cac8I GCNNGC 1 cut(s) 2305
CaiI CAGNNNCTG 2 cut(s) 301, 934
CciI TCATGA 3 cut(s) 1396, 1429, 2206
CfoI GCGC 1 cut(s) 2689
Cfr10I RCCGGY 2 cut(s) 1111, 1249
Cfr13I GGNCC 2 cut(s) 2074, 2156
Csp6I GTAC 3 cut(s) 484, 1945, 1975
CspAI ACCGGT 2 cut(s) 1111, 1249
CviQI GTAC 3 cut(s) 484, 1945, 1975
DraI TTTAAA 1 cut(s) 1821
DraIII CACNNNGTG 1 cut(s) 1055
EaeI YGGCCR 1 cut(s) 2497
Eam1104I CTCTTC 9 cut(s) 122, 1521, 1617, 1842, 2238, 2310, 2349, 2376, 2670
EarI CTCTTC 9 cut(s) 122, 1521, 1617, 1842, 2238, 2310, 2349, 2376, 2670
Ecl136II GAGCTC 2 cut(s) 878, 2008
Eco130I CCWWGG 2 cut(s) 852, 2267
Eco24I GRGCYC 3 cut(s) 207, 880, 2010
Eco31I GGTCTC 1 cut(s) 1375
Eco47I GGWCC 2 cut(s) 2074, 2156
Eco53kI GAGCTC 2 cut(s) 878, 2008
Eco57I CTGAAG 1 cut(s) 2613
Eco88I CYCGRG 1 cut(s) 2233
EcoICRI GAGCTC 2 cut(s) 878, 2008
EcoRI GAATTC 3 cut(s) 685, 1538, 2842
EcoRII CCWGG 1 cut(s) 1980
EcoT14I CCWWGG 2 cut(s) 852, 2267
EcoT22I ATGCAT 2 cut(s) 1092, 1230
EcoT38I GRGCYC 3 cut(s) 207, 880, 2010
ErhI CCWWGG 2 cut(s) 852, 2267
FalI AAGNNNNNCTT 4 cut(s) 2131, 2163, 2346, 2378
FaqI GGGAC 2 cut(s) 1393, 2250
FbaI TGATCA 4 cut(s) 589, 949, 1393, 2419
Fnu4HI GCNGC 7 cut(s) 6, 415, 457, 1872, 1920, 1994, 2131
FriOI GRGCYC 3 cut(s) 207, 880, 2010
Fsp4HI GCNGC 7 cut(s) 6, 415, 457, 1872, 1920, 1994, 2131
FspBI CTAG 5 cut(s) 183, 1535, 2054, 2304, 2333
FspI TGCGCA 1 cut(s) 2688
GlaI GCGC 1 cut(s) 2688
GluI GCNGC 7 cut(s) 6, 415, 457, 1872, 1920, 1994, 2131
GsuI CTGGAG 1 cut(s) 956
HaeIII GGCC 5 cut(s) 156, 815, 890, 2090, 2499
HapII CCGG 4 cut(s) 207, 974, 1112, 1250
HhaI GCGC 1 cut(s) 2689
Hin6I GCGC 1 cut(s) 2687
HinP1I GCGC 1 cut(s) 2687
HindIII AAGCTT 3 cut(s) 1815, 1935, 2360
HpaII CCGG 4 cut(s) 207, 974, 1112, 1250
HphI GGTGA 8 cut(s) 284, 599, 875, 1010, 1772, 2474, 2486, 2777
Hpy166II GTNNAC 1 cut(s) 728
Hpy8I GTNNAC 1 cut(s) 728
HpyAV CCTTC 9 cut(s) 113, 519, 578, 1479, 1621, 1695, 2065, 2209, 2621
HpyCH4III ACNGT 3 cut(s) 224, 1133, 2744
HpyF10VI GCNNNNNNNGC 3 cut(s) 465, 1871, 2717
HspAI GCGC 1 cut(s) 2687
Ksp22I TGATCA 4 cut(s) 589, 949, 1393, 2419
LmnI GCTCC 2 cut(s) 2050, 2756
Lsp1109I GCAGC 5 cut(s) 443, 1883, 1931, 2005, 2117
LweI GCATC 7 cut(s) 559, 733, 934, 940, 1730, 2263, 2654
MaeI CTAG 5 cut(s) 183, 1535, 2054, 2304, 2333
MaeIII GTNAC 5 cut(s) 27, 782, 1016, 2765, 2810
MfeI CAATTG 1 cut(s) 2415
MflI RGATCY 3 cut(s) 235, 1465, 2779
MhlI GDGCHC 4 cut(s) 207, 880, 1051, 2010
MlsI TGGCCA 1 cut(s) 2499
MluNI TGGCCA 1 cut(s) 2499
MlyI GAGTC 3 cut(s) 317, 1196, 1776
MmeI TCCRAC 1 cut(s) 576
Mox20I TGGCCA 1 cut(s) 2499
Mph1103I ATGCAT 2 cut(s) 1092, 1230
MroXI GAANNNNTTC 2 cut(s) 84, 1530
MscI TGGCCA 1 cut(s) 2499
MseI TTAA 7 cut(s) 435, 1311, 1608, 1820, 2000, 2556, 2756
MslI CAYNNNNRTG 4 cut(s) 258, 2457, 2694, 2808
Msp20I TGGCCA 1 cut(s) 2499
MspCI CTTAAG 1 cut(s) 2755
MspI CCGG 4 cut(s) 207, 974, 1112, 1250
MspR9I CCNGG 2 cut(s) 207, 1982
MunI CAATTG 1 cut(s) 2415
Mva1269I GAATGC 1 cut(s) 1689
MvaI CCWGG 1 cut(s) 1982
MwoI GCNNNNNNNGC 3 cut(s) 465, 1871, 2717
NciI CCSGG 1 cut(s) 207
NcoI CCATGG 1 cut(s) 852
NheI GCTAGC 1 cut(s) 2303
NlaIV GGNNCC 1 cut(s) 972
NmuCI GTSAC 4 cut(s) 782, 1016, 2765, 2810
NsbI TGCGCA 1 cut(s) 2688
NsiI ATGCAT 2 cut(s) 1092, 1230
NspI RCATGY 3 cut(s) 771, 1090, 2693
PagI TCATGA 3 cut(s) 1396, 1429, 2206
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 1689
PdmI GAANNNNTTC 2 cut(s) 84, 1530
PfeI GAWTC 8 cut(s) 58, 289, 406, 989, 1706, 2203, 2294, 2636
PflFI GACNNNGTC 1 cut(s) 788
PflMI CCANNNNNTGG 2 cut(s) 301, 1885
PinAI ACCGGT 2 cut(s) 1111, 1249
PkrI GCNGC 7 cut(s) 7, 416, 458, 1873, 1921, 1995, 2132
PleI GAGTC 3 cut(s) 316, 1196, 1775
PpsI GAGTC 3 cut(s) 316, 1196, 1775
PsiI TTATAA 1 cut(s) 1029
Psp124BI GAGCTC 2 cut(s) 880, 2010
Psp6I CCWGG 1 cut(s) 1980
PspGI CCWGG 1 cut(s) 1980
PspN4I GGNNCC 1 cut(s) 972
PspPI GGNCC 2 cut(s) 2074, 2156
PstNI CAGNNNCTG 2 cut(s) 301, 934
PsuI RGATCY 3 cut(s) 235, 1465, 2779
PsyI GACNNNGTC 1 cut(s) 788
RsaI GTAC 3 cut(s) 485, 1946, 1976
RsaNI GTAC 3 cut(s) 484, 1945, 1975
RseI CAYNNNNRTG 4 cut(s) 258, 2457, 2694, 2808
SacI GAGCTC 2 cut(s) 880, 2010
SaqAI TTAA 7 cut(s) 435, 1311, 1608, 1820, 2000, 2556, 2756
SatI GCNGC 7 cut(s) 6, 415, 457, 1872, 1920, 1994, 2131
Sau96I GGNCC 2 cut(s) 2074, 2156
SchI GAGTC 3 cut(s) 317, 1196, 1776
ScrFI CCNGG 2 cut(s) 207, 1982
SduI GDGCHC 4 cut(s) 207, 880, 1051, 2010
SfaNI GCATC 7 cut(s) 559, 733, 934, 940, 1730, 2263, 2654
SfcI CTRYAG 1 cut(s) 1653
SinI GGWCC 2 cut(s) 2074, 2156
SmiMI CAYNNNNRTG 4 cut(s) 258, 2457, 2694, 2808
SmlI CTYRAG 7 cut(s) 312, 351, 469, 503, 1062, 2342, 2755
SmoI CTYRAG 7 cut(s) 312, 351, 469, 503, 1062, 2342, 2755
SsiI CCGC 4 cut(s) 414, 459, 2276, 2472
SspI AATATT 1 cut(s) 347
SspMI CTAG 5 cut(s) 183, 1535, 2054, 2304, 2333
SstI GAGCTC 2 cut(s) 880, 2010
StyD4I CCNGG 2 cut(s) 205, 1980
StyI CCWWGG 2 cut(s) 852, 2267
TaaI ACNGT 3 cut(s) 224, 1133, 2744
TaqII GACCGA 1 cut(s) 1059
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 8 cut(s) 58, 289, 406, 989, 1706, 2203, 2294, 2636
Tru1I TTAA 7 cut(s) 435, 1311, 1608, 1820, 2000, 2556, 2756
Tru9I TTAA 7 cut(s) 435, 1311, 1608, 1820, 2000, 2556, 2756
TscAI CASTG 2 cut(s) 1459, 2747
TseFI GTSAC 4 cut(s) 782, 1016, 2765, 2810
TseI GCWGC 6 cut(s) 5, 456, 1871, 1919, 1993, 2130
Tsp45I GTSAC 4 cut(s) 782, 1016, 2765, 2810
TspGWI ACGGA 3 cut(s) 372, 818, 1825
TspRI CASTG 2 cut(s) 1459, 2747
Tth111I GACNNNGTC 1 cut(s) 788
Van91I CCANNNNNTGG 2 cut(s) 301, 1885
Vha464I CTTAAG 1 cut(s) 2755
VpaK11BI GGWCC 2 cut(s) 2074, 2156
XapI RAATTY 5 cut(s) 685, 882, 1538, 2793, 2842
XbaI TCTAGA 2 cut(s) 1534, 2332
XceI RCATGY 3 cut(s) 771, 1090, 2693
XcmI CCANNNNNNNNNTGG 1 cut(s) 1882
XmnI GAANNNNTTC 2 cut(s) 84, 1530
XspI CTAG 5 cut(s) 183, 1535, 2054, 2304, 2333
Zsp2I ATGCAT 2 cut(s) 1092, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.