Rh4DG411300

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011757
Physical Location & Seq
Reverse (-)
62295841 .. 62301400
5560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG411300.1

Sequence Viewer

Length: 3318 bp
ATGGCTGCCAACAAGTTTGCCACCATGTTACACAGAAACACCAACAAACTCACTCTGATTCTCATCTACGCAATCCTCGAATGGGTTCTGATCCTCCTCCTCCTTCTCAACTCCCTCTTCTCTTATCTGATCGTCAAGTTCGCCGATTACTTTGGCCTCAAACGACCCTGCCTCTGGTGTTCTAGGCTCGATCACATCTTCGAGCCCGGAAACAAGAGCCACAGCTCTTACAGAGATCTTGTGTGTGAAGAACACGCCAATGAAATAGCCAAACTTGGGTTTTGCTCGAATCACCAGAAACTGGCTGAGTCTCAAGATATGTGTGAGGATTGCTCATCGCAGGAATATTCTCAAGAATGGTCTAACAAGTATGCTTTCTTTCCGTGGATGAAGCAAATTGGGATGATTCAAGGCGGCGATGATGATAAGGAGATTAACGATAAGGAGTGTTCTTGCTGCGGTGTGAGCTTGAGCCACAAGCTGTACCCTCCTTGTATTCTGCTCAAGCCTTCTTGGGGGGTTTTGGATTACGCTCAAAAACCGATTTTGAGTTCAGATGTAGGGGTTGATGCTCAGACTGAGGAAGGTGATCAGTCGGATCAAAGTGGATCGGATTATGTGGCAGATCAAAATGGACATGATGAGGGTGTTGTTGTTGTTGTTGTTCAAGGAAATAGGGGAGAGAATTCGGTTTCGGTTTCGGATGTTGATGGATGTTGTGGACAAAGAGACTCTGATGCAGAGGAGAAGAATAATTCAGCATGTTGTTCTGTTTGTGACTATGTCTGCAAGGAAACGGAAACTGAGGCCAATCAAATTGAAGACGAGGCTAATATGAATGTCTCCATGGATGATGGTGATGATGGTCGAGCTCAAATTCAGGCCAGTTGTGATGAGAATAAGTCTCGTGGGAGTCTGCATCAGCATCTGGAGTTCTACATTGATCAAGATGATTGCCGGTTGGTTCCGGTTGAATTGGTTGATTCTCCTGCCACCATCGAGAGGCAAAGTCACCAGAATTATAAGGTGGAGGAAGACCGAGCACATAGTGATACTCAAGATGTTATTCTGGATTTCGACATGCATTTCGAGACACAAGCCAAACCGGTTGTTGAGTTGTGGCACAGTTCAGATGAAATTGTGGAATTACTTTCATCCCATGAGAGTAAAGAGGAGAGCAAAGTTTCAGTTCTTGACTCAAAGGATTTAGATTTCGATATGCATTTCAAGACACAAGCCAAACCGGTTCTTGAGTGTTGGCAGAGTTCAGATGAGATTGTGGAATTACTTTCATCCCATGAGAATAAAGAGGAGAGCAAAGTTTCAGTTCTTGACTCAAAGGATTTGGATTTCGATATGCATTTCAAGACACAAGCCAAACCGGTTGTTGAGTGTTGGCAGAGTTCAGATGAGATTGTGGAATTACTTTCATCCCATGAGATTAAAGAGGAGAGCAAAGTTTCAGTTCTTGACTCAAAGGATTTGGATTTCGATATGCATTTTGAGACGCAACCCAAACCGGTTGTTGAGTGTTGGCCCAGTTCAGATGAGATTGTGGTACTTTCATCCCATGAGATTAAAGAGGAGAACAAAGTTTCAGTTCTTGACTCAAAGGATTTGGGCCAGAATGGAGAAGAAGTTGAAGAAAATGAGCAAGCTGAAGATACTACTAGTACTCAAGCAACATCAGCCTCTTGTCATGAGGATGACAGTCAATCAAATGCTGCTGACATAGTAGGAAGAGACATTGATTCAGATGTTCTTCAAGCAACAACTGAGGATGATGACATCCATATGCGGAACGATGAGATTGATACAGAGGTCTCAATAGGGACTGATATTCCTGATCATGATATATTCAATGAGGATATTCAATCAGCTCATGAACTTCTACATTCTTATCCCAGTGTGCAAGAAGATCCTTCTACAAGTTCTCCCAATGCTGAAATTGATGATAATCATGGTGCTAAGCAAGAAGAAGAGTTTCTAGAATTCAAGACCTTATCAGTTGAAACAAGTGAGAAAGAACAAAATAGTTATTTCTCATTAGGTTCAGAACTTAATGAGATTGAGGAAGAGAAGGTTCTTCCTGATACACCAACTTCTATAGACAATTTCCATCAGTTGTATAATAGAATGCTACTAACTGAGAGAAGGGAATCGGGAACAGAAGCATCATTGGATGGAAGTGTAGTGAGCGACATTGAATGTGGTGATGGAGTCCTGACTATGGAGAAGCTGAAATCGGTGTTGAGAGCTGAACGGAAAGCTTTAAATAGAGTATATGCAGAACTTGAAGAAGAGAGGAGTGCTTCTGCAATAGCAGCCAACCAGACAATGGCAATGATAAATAGGCTACAAGAAGAAAAGGCAGCAATGCAGATGGAAGCTTTGCAGTACCAAAGGATGATGGATGAGCAGTCTGAGTACGACCAGGAAGCTATGCAGCTCTTAAACGAGCTCATGGTAAAGAGGGAAAAGGAAAAGCAAGAACTTGAAAAGGAGCTAGAAATATGTCGAAAGAAGGTCCAAGATTATGAGGCCAAAGAGAAAATTATGATTTTGAGGAGAATGAAAGAGGGCTGCACAAGAAGTAGGACTTCATCTGGTCCTTGTAGCAATGCTGAGGATAGTGATGGACTTTCTATTGATTTGAATCATGAAGTGAAGGAAGAAGATAACCTCTCGGGACATGAAGAGAGTAGCAATCAAAACACACCAAGGGATGCGGTGTTATATTTAGAAGAATCTCTGGCTAGCTTTGAGGAAGAGAAGTTATCCATTCTAGACCAACTCAAGGAATTGGAAGAGAAGCTTTTGACCTTGAATGACGAAGAGGAAGAAGAACATTACGAGGATATAAAACCAATTGATCATTTCTTTTCAGAGAATGGGAATGGCTACCATGAGAATGGTGATGTTGGCGGTGAAGAAAATGGCATTGCAAATGGCCACTCCAAAGAAATGAATGGAACACATCATCAAGATAGAAAAATTATGGGATCAATTAAGGCAAAGAGACTTCTTCCACTTTTCAATGAAGCTGAAGATGAAGACGAGGAGTTGAATGGAGATGTAGAAGGTTATGATTCTGTTGCATCACAAGATCACAAGAAATTCGCTATTGAAGAGGAGGTTGCGCATGTCTATGAGAGACTACAAGCACTTGAAGCAGATAGAGAGTTTCTAAAGCACTGTATTAGCTCCTTAAGGAAAGGTGACAAGGGATTAGATCTTCTCCAAGAAATTTTGCAACATCTTCGTGACCTGAGAAGTGTGGAAACCCGTCTAAGGAATTCGGGAGAGGGTATCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1105

Amino Acids

125.23

Weight (kDa)

4.43

Isoelectric Point (pI)

55.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 746 - 836 1.2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
Acc16I TGCGCA 1 cut(s) 3141
AccB7I CCANNNNNTGG 2 cut(s) 301, 2338
AciI CCGC 5 cut(s) 414, 459, 1798, 2729, 2925
AclWI GGATC 5 cut(s) 85, 606, 616, 1913, 3010
AcoI YGGCCR 1 cut(s) 2950
AcsI RAATTY 6 cut(s) 685, 876, 1991, 3116, 3246, 3295
AcuI CTGAAG 2 cut(s) 1680, 3066
AdeI CACNNNGTG 1 cut(s) 1049
AfaI GTAC 5 cut(s) 485, 1560, 1675, 2399, 2429
AflII CTTAAG 1 cut(s) 3208
AgeI ACCGGT 4 cut(s) 1105, 1243, 1381, 1519
AhlI ACTAGT 1 cut(s) 1670
AjnI CCWGG 1 cut(s) 2433
Alw21I GWGCWC 3 cut(s) 874, 1045, 2463
AlwI GGATC 5 cut(s) 85, 606, 616, 1913, 3010
AlwNI CAGNNNCTG 2 cut(s) 301, 928
Ama87I CYCGRG 1 cut(s) 2686
AoxI GGCC 7 cut(s) 154, 807, 882, 1535, 1621, 2541, 2950
ApeKI GCWGC 7 cut(s) 5, 456, 1724, 2324, 2372, 2446, 2583
ApoI RAATTY 6 cut(s) 685, 876, 1991, 3116, 3246, 3295
ArsI GACNNNNNNTTYG 2 cut(s) 1070, 1102
AsiGI ACCGGT 4 cut(s) 1105, 1243, 1381, 1519
Asp700I GAANNNNTTC 2 cut(s) 84, 1983
AspLEI GCGC 1 cut(s) 3142
AspS9I GGNCC 4 cut(s) 1536, 1621, 2527, 2609
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 8 cut(s) 284, 599, 869, 1004, 2225, 2927, 2939, 3230
AsuNHI GCTAGC 1 cut(s) 2756
AvaI CYCGRG 1 cut(s) 2686
AvaII GGWCC 2 cut(s) 2527, 2609
BaeI ACNNNNGTAYC 2 cut(s) 1806, 1839
BalI TGGCCA 1 cut(s) 2952
BanII GRGCYC 3 cut(s) 207, 874, 2463
BauI CACGAG 1 cut(s) 906
BbsI GAAGAC 3 cut(s) 828, 1041, 3060
Bbv12I GWGCWC 3 cut(s) 874, 1045, 2463
BbvCI CCTCAGC 1 cut(s) 2625
BbvI GCAGC 6 cut(s) 443, 1711, 2336, 2384, 2458, 2570
BcgI CGANNNNNNTGC 2 cut(s) 3242, 3276
BciT130I CCWGG 1 cut(s) 2435
BclI TGATCA 4 cut(s) 589, 943, 1846, 2872
BcnI CCSGG 1 cut(s) 207
BcuI ACTAGT 1 cut(s) 1670
BfaI CTAG 6 cut(s) 183, 1671, 1988, 2507, 2757, 2786
BfmI CTRYAG 1 cut(s) 2106
BfrI CTTAAG 1 cut(s) 3208
BglII AGATCT 2 cut(s) 235, 3232
BisI GCNGC 8 cut(s) 6, 415, 457, 1725, 2325, 2373, 2447, 2584
BlpI GCTNAGC 1 cut(s) 1968
BlsI GCNGC 8 cut(s) 7, 416, 458, 1726, 2326, 2374, 2448, 2585
BmcAI AGTACT 1 cut(s) 1675
Bme1390I CCNGG 2 cut(s) 207, 2435
Bme18I GGWCC 2 cut(s) 2527, 2609
BmeT110I CYCGRG 1 cut(s) 2686
BmgT120I GGNCC 4 cut(s) 1536, 1621, 2527, 2609
BmiI GGNNCC 1 cut(s) 966
BmrFI CCNGG 2 cut(s) 207, 2435
BmrI ACTGGG 2 cut(s) 1533, 1899
BmsI GCATC 7 cut(s) 559, 727, 928, 934, 2183, 2716, 3107
BmtI GCTAGC 1 cut(s) 2760
BmuI ACTGGG 2 cut(s) 1533, 1899
BpiI GAAGAC 3 cut(s) 828, 1041, 3060
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 950
Bpu10I CCTNAGC 1 cut(s) 2625
Bpu1102I GCTNAGC 1 cut(s) 1968
BpuEI CTTGAG 8 cut(s) 297, 336, 488, 490, 1041, 1271, 1662, 2780
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 3 cut(s) 62, 1956, 2655
BsaI GGTCTC 1 cut(s) 1828
BsaJI CCNNGG 3 cut(s) 383, 846, 2720
BsaWI WCCGGW 5 cut(s) 967, 1105, 1243, 1381, 1519
BsaXI ACNNNNNCTCC 4 cut(s) 1623, 1653, 1918, 1948
Bse118I RCCGGY 5 cut(s) 957, 1105, 1243, 1381, 1519
Bse1I ACTGG 4 cut(s) 306, 885, 1539, 1905
Bse3DI GCAATG 4 cut(s) 2349, 2382, 2626, 2940
Bse8I GATNNNNATC 3 cut(s) 62, 1956, 2655
BseBI CCWGG 1 cut(s) 2435
BseDI CCNNGG 3 cut(s) 383, 846, 2720
BseJI GATNNNNATC 3 cut(s) 62, 1956, 2655
BseMI GCAATG 4 cut(s) 2349, 2382, 2626, 2940
BseMII CTCAG 9 cut(s) 297, 570, 587, 795, 1767, 2139, 2415, 2616, 3260
BseNI ACTGG 4 cut(s) 306, 885, 1539, 1905
BseXI GCAGC 6 cut(s) 443, 1711, 2336, 2384, 2458, 2570
BsgI GTGCAG 1 cut(s) 2569
BshFI GGCC 7 cut(s) 156, 809, 884, 1537, 1623, 2543, 2952
BshTI ACCGGT 4 cut(s) 1105, 1243, 1381, 1519
BsiHKAI GWGCWC 3 cut(s) 874, 1045, 2463
BsiHKCI CYCGRG 1 cut(s) 2686
BsiSI CCGG 7 cut(s) 207, 958, 968, 1106, 1244, 1382, 1520
BslFI GGGAC 2 cut(s) 1846, 2703
BsmBI CGTCTC 1 cut(s) 1499
BsmFI GGGAC 2 cut(s) 1846, 2703
BsmI GAATGC 1 cut(s) 2142
BsnI GGCC 7 cut(s) 156, 809, 884, 1537, 1623, 2543, 2952
Bso31I GGTCTC 1 cut(s) 1828
BsoBI CYCGRG 1 cut(s) 2686
Bsp1286I GDGCHC 4 cut(s) 207, 874, 1045, 2463
Bsp1720I GCTNAGC 1 cut(s) 1968
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 5 cut(s) 414, 459, 1798, 2729, 2925
BspANI GGCC 7 cut(s) 156, 809, 884, 1537, 1623, 2543, 2952
BspCNI CTCAG 9 cut(s) 298, 571, 586, 796, 1768, 2140, 2416, 2617, 3261
BspHI TCATGA 4 cut(s) 1699, 1849, 1882, 2659
BspLI GGNNCC 1 cut(s) 966
BspOI GCTAGC 1 cut(s) 2760
BspPI GGATC 5 cut(s) 85, 606, 616, 1913, 3010
BspTI CTTAAG 1 cut(s) 3208
BspTNI GGTCTC 1 cut(s) 1828
BsrDI GCAATG 4 cut(s) 2349, 2382, 2626, 2940
BsrFI RCCGGY 5 cut(s) 957, 1105, 1243, 1381, 1519
BsrI ACTGG 4 cut(s) 306, 885, 1539, 1905
BssAI RCCGGY 5 cut(s) 957, 1105, 1243, 1381, 1519
BssECI CCNNGG 3 cut(s) 383, 846, 2720
BssSI CACGAG 1 cut(s) 906
BssT1I CCWWGG 2 cut(s) 846, 2720
Bst2BI CACGAG 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 2435
Bst4CI ACNGT 3 cut(s) 1127, 1712, 3197
BstAFI CTTAAG 1 cut(s) 3208
BstC8I GCNNGC 2 cut(s) 1656, 2758
BstDSI CCRYGG 2 cut(s) 383, 846
BstHHI GCGC 1 cut(s) 3142
BstMWI GCNNNNNNNGC 4 cut(s) 465, 1688, 2324, 3170
BstNI CCWGG 1 cut(s) 2435
BstNSI RCATGY 3 cut(s) 765, 1084, 3146
BstSCI CCNGG 2 cut(s) 205, 2433
BstSFI CTRYAG 1 cut(s) 2106
BstV1I GCAGC 6 cut(s) 443, 1711, 2336, 2384, 2458, 2570
BstV2I GAAGAC 3 cut(s) 828, 1041, 3060
BstX2I RGATCY 3 cut(s) 235, 1918, 3232
BstXI CCANNNNNNTGG 1 cut(s) 2912
BstYI RGATCY 3 cut(s) 235, 1918, 3232
BsuRI GGCC 7 cut(s) 156, 809, 884, 1537, 1623, 2543, 2952
BtgI CCRYGG 2 cut(s) 383, 846
BtgZI GCGATG 2 cut(s) 321, 432
BtsIMutI CAGTG 2 cut(s) 1912, 3193
Cac8I GCNNGC 2 cut(s) 1656, 2758
CaiI CAGNNNCTG 2 cut(s) 301, 928
CciI TCATGA 4 cut(s) 1699, 1849, 1882, 2659
CfoI GCGC 1 cut(s) 3142
Cfr10I RCCGGY 5 cut(s) 957, 1105, 1243, 1381, 1519
Cfr13I GGNCC 4 cut(s) 1536, 1621, 2527, 2609
CseI GACGC 1 cut(s) 1516
Csp6I GTAC 5 cut(s) 484, 1559, 1674, 2398, 2428
CspAI ACCGGT 4 cut(s) 1105, 1243, 1381, 1519
CviQI GTAC 5 cut(s) 484, 1559, 1674, 2398, 2428
DraI TTTAAA 1 cut(s) 2274
DraIII CACNNNGTG 1 cut(s) 1049
EaeI YGGCCR 1 cut(s) 2950
Ecl136II GAGCTC 2 cut(s) 872, 2461
Eco130I CCWWGG 2 cut(s) 846, 2720
Eco24I GRGCYC 3 cut(s) 207, 874, 2463
Eco31I GGTCTC 1 cut(s) 1828
Eco47I GGWCC 2 cut(s) 2527, 2609
Eco53kI GAGCTC 2 cut(s) 872, 2461
Eco57I CTGAAG 2 cut(s) 1680, 3066
Eco88I CYCGRG 1 cut(s) 2686
EcoICRI GAGCTC 2 cut(s) 872, 2461
EcoRI GAATTC 3 cut(s) 685, 1991, 3295
EcoRII CCWGG 1 cut(s) 2433
EcoT14I CCWWGG 2 cut(s) 846, 2720
EcoT22I ATGCAT 4 cut(s) 1086, 1224, 1362, 1500
EcoT38I GRGCYC 3 cut(s) 207, 874, 2463
ErhI CCWWGG 2 cut(s) 846, 2720
Esp3I CGTCTC 1 cut(s) 1499
FalI AAGNNNNNCTT 4 cut(s) 2584, 2616, 2799, 2831
FaqI GGGAC 2 cut(s) 1846, 2703
FauNDI CATATG 1 cut(s) 1794
FbaI TGATCA 4 cut(s) 589, 943, 1846, 2872
Fnu4HI GCNGC 8 cut(s) 6, 415, 457, 1725, 2325, 2373, 2447, 2584
FriOI GRGCYC 3 cut(s) 207, 874, 2463
Fsp4HI GCNGC 8 cut(s) 6, 415, 457, 1725, 2325, 2373, 2447, 2584
FspBI CTAG 6 cut(s) 183, 1671, 1988, 2507, 2757, 2786
FspI TGCGCA 1 cut(s) 3141
GlaI GCGC 1 cut(s) 3141
GluI GCNGC 8 cut(s) 6, 415, 457, 1725, 2325, 2373, 2447, 2584
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 7 cut(s) 156, 809, 884, 1537, 1623, 2543, 2952
HapII CCGG 7 cut(s) 207, 958, 968, 1106, 1244, 1382, 1520
HgaI GACGC 1 cut(s) 1516
HhaI GCGC 1 cut(s) 3142
Hin6I GCGC 1 cut(s) 3140
HinP1I GCGC 1 cut(s) 3140
HindIII AAGCTT 3 cut(s) 2268, 2388, 2813
HpaII CCGG 7 cut(s) 207, 958, 968, 1106, 1244, 1382, 1520
HphI GGTGA 8 cut(s) 284, 599, 869, 1004, 2225, 2927, 2939, 3230
Hpy166II GTNNAC 1 cut(s) 722
Hpy8I GTNNAC 1 cut(s) 722
HpyAV CCTTC 9 cut(s) 113, 519, 578, 1932, 2074, 2148, 2518, 2662, 3074
HpyCH4III ACNGT 3 cut(s) 1127, 1712, 3197
HpyF10VI GCNNNNNNNGC 4 cut(s) 465, 1688, 2324, 3170
HspAI GCGC 1 cut(s) 3140
Ksp22I TGATCA 4 cut(s) 589, 943, 1846, 2872
LmnI GCTCC 2 cut(s) 2503, 3209
Lsp1109I GCAGC 6 cut(s) 443, 1711, 2336, 2384, 2458, 2570
LweI GCATC 7 cut(s) 559, 727, 928, 934, 2183, 2716, 3107
MaeI CTAG 6 cut(s) 183, 1671, 1988, 2507, 2757, 2786
MaeIII GTNAC 5 cut(s) 27, 776, 1010, 3218, 3263
MfeI CAATTG 1 cut(s) 2868
MflI RGATCY 3 cut(s) 235, 1918, 3232
MhlI GDGCHC 4 cut(s) 207, 874, 1045, 2463
MlsI TGGCCA 1 cut(s) 2952
MluNI TGGCCA 1 cut(s) 2952
MlyI GAGTC 8 cut(s) 317, 725, 922, 1190, 1328, 1466, 1601, 2229
MmeI TCCRAC 1 cut(s) 576
Mox20I TGGCCA 1 cut(s) 2952
Mph1103I ATGCAT 4 cut(s) 1086, 1224, 1362, 1500
MroXI GAANNNNTTC 2 cut(s) 84, 1983
MscI TGGCCA 1 cut(s) 2952
MseI TTAA 8 cut(s) 435, 1443, 1578, 2061, 2273, 2453, 3009, 3209
MslI CAYNNNNRTG 6 cut(s) 258, 1704, 1793, 2910, 3147, 3261
Msp20I TGGCCA 1 cut(s) 2952
MspCI CTTAAG 1 cut(s) 3208
MspI CCGG 7 cut(s) 207, 958, 968, 1106, 1244, 1382, 1520
MspR9I CCNGG 2 cut(s) 207, 2435
MunI CAATTG 1 cut(s) 2868
Mva1269I GAATGC 1 cut(s) 2142
MvaI CCWGG 1 cut(s) 2435
MwoI GCNNNNNNNGC 4 cut(s) 465, 1688, 2324, 3170
NciI CCSGG 1 cut(s) 207
NcoI CCATGG 1 cut(s) 846
NdeI CATATG 1 cut(s) 1794
NheI GCTAGC 1 cut(s) 2756
NlaIV GGNNCC 1 cut(s) 966
NmuCI GTSAC 4 cut(s) 776, 1010, 3218, 3263
NsbI TGCGCA 1 cut(s) 3141
NsiI ATGCAT 4 cut(s) 1086, 1224, 1362, 1500
NspI RCATGY 3 cut(s) 765, 1084, 3146
PagI TCATGA 4 cut(s) 1699, 1849, 1882, 2659
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 2142
PdmI GAANNNNTTC 2 cut(s) 84, 1983
PfeI GAWTC 9 cut(s) 58, 289, 406, 983, 1751, 2159, 2656, 2747, 3089
PflFI GACNNNGTC 1 cut(s) 782
PflMI CCANNNNNTGG 2 cut(s) 301, 2338
PinAI ACCGGT 4 cut(s) 1105, 1243, 1381, 1519
PkrI GCNGC 8 cut(s) 7, 416, 458, 1726, 2326, 2374, 2448, 2585
PleI GAGTC 8 cut(s) 316, 725, 921, 1190, 1328, 1466, 1601, 2228
PpsI GAGTC 8 cut(s) 316, 725, 921, 1190, 1328, 1466, 1601, 2228
PsiI TTATAA 1 cut(s) 1023
Psp124BI GAGCTC 2 cut(s) 874, 2463
Psp6I CCWGG 1 cut(s) 2433
PspGI CCWGG 1 cut(s) 2433
PspN4I GGNNCC 1 cut(s) 966
PspPI GGNCC 4 cut(s) 1536, 1621, 2527, 2609
PstNI CAGNNNCTG 2 cut(s) 301, 928
PsuI RGATCY 3 cut(s) 235, 1918, 3232
PsyI GACNNNGTC 1 cut(s) 782
RsaI GTAC 5 cut(s) 485, 1560, 1675, 2399, 2429
RsaNI GTAC 5 cut(s) 484, 1559, 1674, 2398, 2428
RseI CAYNNNNRTG 6 cut(s) 258, 1704, 1793, 2910, 3147, 3261
SacI GAGCTC 2 cut(s) 874, 2463
SaqAI TTAA 8 cut(s) 435, 1443, 1578, 2061, 2273, 2453, 3009, 3209
SatI GCNGC 8 cut(s) 6, 415, 457, 1725, 2325, 2373, 2447, 2584
Sau96I GGNCC 4 cut(s) 1536, 1621, 2527, 2609
ScaI AGTACT 1 cut(s) 1675
SchI GAGTC 8 cut(s) 317, 725, 922, 1190, 1328, 1466, 1601, 2229
ScrFI CCNGG 2 cut(s) 207, 2435
SduI GDGCHC 4 cut(s) 207, 874, 1045, 2463
SfaNI GCATC 7 cut(s) 559, 727, 928, 934, 2183, 2716, 3107
SfcI CTRYAG 1 cut(s) 2106
SinI GGWCC 2 cut(s) 2527, 2609
SmiMI CAYNNNNRTG 6 cut(s) 258, 1704, 1793, 2910, 3147, 3261
SmlI CTYRAG 9 cut(s) 312, 351, 469, 503, 1056, 1250, 1677, 2795, 3208
SmoI CTYRAG 9 cut(s) 312, 351, 469, 503, 1056, 1250, 1677, 2795, 3208
SpeI ACTAGT 1 cut(s) 1670
SsiI CCGC 5 cut(s) 414, 459, 1798, 2729, 2925
SspI AATATT 1 cut(s) 347
SspMI CTAG 6 cut(s) 183, 1671, 1988, 2507, 2757, 2786
SstI GAGCTC 2 cut(s) 874, 2463
StyD4I CCNGG 2 cut(s) 205, 2433
StyI CCWWGG 2 cut(s) 846, 2720
TaaI ACNGT 3 cut(s) 1127, 1712, 3197
TaqII GACCGA 1 cut(s) 1053
TatI WGTACW 1 cut(s) 1673
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 9 cut(s) 58, 289, 406, 983, 1751, 2159, 2656, 2747, 3089
Tru1I TTAA 8 cut(s) 435, 1443, 1578, 2061, 2273, 2453, 3009, 3209
Tru9I TTAA 8 cut(s) 435, 1443, 1578, 2061, 2273, 2453, 3009, 3209
TscAI CASTG 2 cut(s) 1912, 3200
TseFI GTSAC 4 cut(s) 776, 1010, 3218, 3263
TseI GCWGC 7 cut(s) 5, 456, 1724, 2324, 2372, 2446, 2583
Tsp45I GTSAC 4 cut(s) 776, 1010, 3218, 3263
TspGWI ACGGA 3 cut(s) 372, 812, 2278
TspRI CASTG 2 cut(s) 1912, 3200
Tth111I GACNNNGTC 1 cut(s) 782
Van91I CCANNNNNTGG 2 cut(s) 301, 2338
Vha464I CTTAAG 1 cut(s) 3208
VpaK11BI GGWCC 2 cut(s) 2527, 2609
XapI RAATTY 6 cut(s) 685, 876, 1991, 3116, 3246, 3295
XbaI TCTAGA 2 cut(s) 1987, 2785
XceI RCATGY 3 cut(s) 765, 1084, 3146
XcmI CCANNNNNNNNNTGG 1 cut(s) 2335
XmnI GAANNNNTTC 2 cut(s) 84, 1983
XspI CTAG 6 cut(s) 183, 1671, 1988, 2507, 2757, 2786
ZrmI AGTACT 1 cut(s) 1675
Zsp2I ATGCAT 4 cut(s) 1086, 1224, 1362, 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.