Rroxscaffold_5G00384100

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011757
Physical Location & Seq
Reverse (-)
63624956 .. 63629390
4435 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00384100.1

Sequence Viewer

Length: 3180 bp
ATGGCTGCCAACAAGTTTGCCACCATGTTACACAGAAACACCAACAAGCTCACTCTGATTCTCATCTACGCAATCCTCGAATGGGTTCTGATCCTCCTCCTCCTTCTCAACTCTCTCTTCTCTTATCTGATCGTCAAGTTCGCCGATTACTTTGGCCTCAAACGACCCTGCCTGTGGTGTTCTAGGCTCGATCACATCTTGGAGCCCGGAAACAAGGGCCACAGTTCTTACAGAGATCTTGTGTGTGAGGAACACGCCAATGAAATAGCCAAACTTGGGTTTTGCTCGAATCACCAGAAACTGGCTGAGTCTCAAGATATGTGTGAGGATTGCTCATCGCAGGAATATTCTCAAGAATGGTCTAACAAGTACGCTTTCTTTCCGTGGATGAAGCAAATTGGGATGATTCAAGGCGGCGATGATGATGATGATGATAAGGAGATTAACGATAAGGAATGTTCTTGCTGCGGTGTGAGCTTGAGCAACAAGCTGTACCCTCCTTGTATTCTGCTCAAGCCTTCTTGGGGGGTTTTGGATTACGCTCAAAAACCGATTTCGAGTTCAGATGTAGGGGTTGATGCTCAGACTGAGGAAGGTGATCAGTCGGATCAAAGTGGATCGGATTATGTGGCAGATCAAAATGGACATGATGAGGGTGTTGTTGTTGTTGTTCAAGGAAATAGGGGAGAGAATTCGGTTTCGGATGTTGATGGATGTTGCGGACAAAGAGACTATGATGCAGAGGAGAAGAATAATTCAGCATGTTGTTCTGTCTGTGACTATGTCTGCAAGGAAACGGAAACTGAGGCCAATCAAATTGAAGACGAGGCTAATACGAATGTCTCCATGGATGACGGTGATGATGGTCGAGCTCAAATTCAGGCCAGTTGTGATGAGAATAAGTCTCGTGCAAGTCTGCATCAGCATCTGGAGTTCTACATTGATCAAGATGATTGTCGGTTGGTTCCGGTTGAATTGGTTGATTCTCCTGCCGCCATTGAGAGGCAAAGTCACCAGAATTATAAGGTGGAGGAAGACCGAGCACATAGTGATACTCAAGATGTTATTCTGGATTTCGACATGCATTTCGAGACACAAGCCAAACCGGTTGTTGAGTTGTGGCACAGTTCAGATGAAATTGTGGAATTACTTTCATCCCATGAGAGTAAAGAGGAGAGAAAAGTTTCAGTTCTTGACTCAAAGGATTTGGATTTCGATATGCATTTCAAGACACAAGCCAAACCGGTTGTTGAGTGTTGGCAGAGTTCAGATGAGATTGTGGAATTACTTTCATCCCATGAGATTAAAGAGGAGAGCAAAGTTTCAGTTCTTGACTCAAAGGGTTTGGATTTCGATATGCATTTTGAGACGCAAGCCAAACCGGTTGTTGAGTGTTGGCCCAGTTCAGATGAGATTGTGGTGCTTTCATCCCATGAGATTAAAGAGGAGAACAAAGTTTCAGTTCCTGACTCAAAGGATTTGGGGCAGAATGGAGAAGAAGTTGAAGAAAATGAGCAAGCTGAAGATACTACTAGTACTCAAGCAACACCAGCCTCTTGTCATGAGGATGACAGTCAATCAAATACTGCTGATATAGTAGGAAGAGACATTGATTCAGATGTTCATCAAGCAACAACTGAGGATGATGACATCCATATGCGGAACGATGAGATTGATACAGAGGTCTCAATAGGGACTGATATTCCTGATCATGATATATTCAATGAGGATATTCAATCAGCTCATGAACTTCTACATTCTTATCCCAGTGTGCAAGAAGATCCTTCTACAAGTTCTCCCAATGCTGAAATTGATGATAATCATGGTGCTAAGCAAGAAGAAGAGTTTCTAGAATTCAAGACCTTATCAGTTGAAACAAGTGAGAAAGAACAAAATAGTTATTTCTCATTAGATTCAGAACTTATGGAGATTGAGGAAGAGAAGGTTCTTCCTGATACACCAACTTCTATAGACAATTTCCATCAGTTGTATAATAGAATGCTACTAACTGAGAGAAGGGAATCGGGAACAGAAGCATCATTGGATGGAAGTGTAGTGAGCGACATTGAATGTGGTGATGGAGTCCTGACTATGGAGAAGCTGAAATCGGTGTTGAGAGCTGAACGGAAAGCTTTAAATAGAGTATATGCAGAACTCGAAGAAGAGAGGAGTGCTTCTGCAATAGCAGCCAACCAGACAATGGCAATGATAAATAGGCTACAAGAAGAAAAGGCAGCAATGCAGATGGAAGCTTTGCAGTACCAAAGAATGATGGATGAGCAGTCTGAGTACGACCAGGAAGCTATGCAGCTCTTAAACGAGCTCATGGTAAAGAGGGAAAAGGAAAAGCAAGAACTTGAAAAGGAGCTAGAAATATGTCGAAAGAAGGTCCAAGATTATGAGGCCAAAGAGAAAATGATGATTTTGAGGAGAATGAAAGAGGGCTGCACAAGAAGTAGGACTTCATCTGGTCCTTGTAGCAATGCTGAGGATAGTGATGGACTTTCTATTGATTTGAATCATGAAGTGAAGGAAGAAGATAACCTCTCGGGACATGAAGAGACTAGCAATCAAAACACGCCAAGGGATGCAGTGTTATATTTAGAAGAATCTTTGGCTAGCTTTGAGGAAGAGAAGTTATCCATTCTAGACCAACTCAAGGAATTGGAAGAGAAGCTTTTGACCTTGAATGACGAAGAGGAAGAAGAACATTATGAGGATATAAAACCAATTGATCATTTCTTTTTAGAGAATGGAAATGGCTACCATGAGAATGGTGATGTTGGCAGTGAAGAAAATGGCATTGCAAATGGCCACTCCAAAGAAATGAATGGAACACATCATCAAGATAGAAAAATTATGGGATCAATTAAGGCAAAGAGACTTCTTCCACTTTTCAATGAAGCTGAAGATGAAGACGAGGAGTTGAATGGAGATGTAGGAGGTTATGATTCTGTTGCATCACAAGATCACAAGCAACTCGCTATTGAAGAGGAGGTTGCGCATGTCTATGAGAGACTACAAGCACTTGAAGCAGATAGAGAGTTTCTAAAGCACTGTATTAGCTCCTTAAGGAAAGGTGACAAGGGATTAGATCTTCTCCAAGAAATTTTGCAACATCTTCGTGACCTGAGAAGTGTGGAAACCCGTCTAAGGAATTCGGGAGAGGGTATCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1059

Amino Acids

119.99

Weight (kDa)

4.42

Isoelectric Point (pI)

54.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 700 - 790 1.1e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
Acc16I TGCGCA 1 cut(s) 3003
AccB7I CCANNNNNTGG 2 cut(s) 301, 2200
AciI CCGC 5 cut(s) 414, 468, 720, 993, 1660
AclWI GGATC 5 cut(s) 85, 615, 625, 1775, 2872
AcoI YGGCCR 1 cut(s) 2812
AcsI RAATTY 5 cut(s) 691, 876, 1853, 3108, 3157
AcuI CTGAAG 2 cut(s) 1542, 2928
AdeI CACNNNGTG 1 cut(s) 1049
AfaI GTAC 5 cut(s) 371, 494, 1537, 2261, 2291
AflII CTTAAG 1 cut(s) 3070
AgeI ACCGGT 3 cut(s) 1105, 1243, 1381
AhlI ACTAGT 1 cut(s) 1532
AjnI CCWGG 1 cut(s) 2295
Alw21I GWGCWC 3 cut(s) 874, 1045, 2325
AlwI GGATC 5 cut(s) 85, 615, 625, 1775, 2872
AlwNI CAGNNNCTG 3 cut(s) 301, 928, 1466
Ama87I CYCGRG 1 cut(s) 2548
AoxI GGCC 7 cut(s) 154, 217, 807, 882, 1397, 2403, 2812
ApeKI GCWGC 6 cut(s) 5, 465, 2186, 2234, 2308, 2445
ApoI RAATTY 5 cut(s) 691, 876, 1853, 3108, 3157
ArsI GACNNNNNNTTYG 2 cut(s) 1070, 1102
AsiGI ACCGGT 3 cut(s) 1105, 1243, 1381
Asp700I GAANNNNTTC 3 cut(s) 84, 1183, 1845
AspLEI GCGC 1 cut(s) 3004
AspS9I GGNCC 4 cut(s) 217, 1398, 2389, 2471
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 7 cut(s) 284, 608, 869, 1004, 2087, 2789, 3092
AsuNHI GCTAGC 1 cut(s) 2618
AvaI CYCGRG 1 cut(s) 2548
AvaII GGWCC 2 cut(s) 2389, 2471
BaeI ACNNNNGTAYC 2 cut(s) 1668, 1701
BalI TGGCCA 1 cut(s) 2814
BanII GRGCYC 3 cut(s) 207, 874, 2325
BauI CACGAG 1 cut(s) 906
BbsI GAAGAC 3 cut(s) 828, 1041, 2922
Bbv12I GWGCWC 3 cut(s) 874, 1045, 2325
BbvCI CCTCAGC 1 cut(s) 2487
BbvI GCAGC 5 cut(s) 452, 2198, 2246, 2320, 2432
BccI CCATC 8 cut(s) 704, 857, 1989, 2039, 2072, 2239, 2266, 2492
BcgI CGANNNNNNTGC 2 cut(s) 3104, 3138
BciT130I CCWGG 1 cut(s) 2297
BclI TGATCA 4 cut(s) 598, 943, 1708, 2734
BcnI CCSGG 1 cut(s) 207
BcuI ACTAGT 1 cut(s) 1532
BfaI CTAG 7 cut(s) 183, 1533, 1850, 2369, 2565, 2619, 2648
BfmI CTRYAG 1 cut(s) 1968
BfrI CTTAAG 1 cut(s) 3070
BglII AGATCT 2 cut(s) 235, 3094
BisI GCNGC 8 cut(s) 6, 415, 466, 993, 2187, 2235, 2309, 2446
BlpI GCTNAGC 1 cut(s) 1830
BlsI GCNGC 8 cut(s) 7, 416, 467, 994, 2188, 2236, 2310, 2447
BmcAI AGTACT 1 cut(s) 1537
Bme1390I CCNGG 2 cut(s) 207, 2297
Bme18I GGWCC 2 cut(s) 2389, 2471
BmeT110I CYCGRG 1 cut(s) 2548
BmgT120I GGNCC 4 cut(s) 217, 1398, 2389, 2471
BmiI GGNNCC 2 cut(s) 204, 966
BmrFI CCNGG 2 cut(s) 207, 2297
BmrI ACTGGG 2 cut(s) 1395, 1761
BmsI GCATC 7 cut(s) 568, 727, 928, 934, 2045, 2578, 2969
BmtI GCTAGC 1 cut(s) 2622
BmuI ACTGGG 2 cut(s) 1395, 1761
BpiI GAAGAC 3 cut(s) 828, 1041, 2922
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 950
Bpu10I CCTNAGC 1 cut(s) 2487
Bpu1102I GCTNAGC 1 cut(s) 1830
BpuEI CTTGAG 7 cut(s) 297, 336, 497, 499, 1041, 1524, 2642
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 4 cut(s) 62, 1623, 1818, 2517
BsaI GGTCTC 1 cut(s) 1690
BsaJI CCNNGG 3 cut(s) 383, 846, 2582
BsaWI WCCGGW 4 cut(s) 967, 1105, 1243, 1381
BsaXI ACNNNNNCTCC 4 cut(s) 1485, 1515, 1780, 1810
Bse118I RCCGGY 3 cut(s) 1105, 1243, 1381
Bse1I ACTGG 4 cut(s) 306, 885, 1401, 1767
Bse3DI GCAATG 4 cut(s) 2211, 2244, 2488, 2802
Bse8I GATNNNNATC 4 cut(s) 62, 1623, 1818, 2517
BseBI CCWGG 1 cut(s) 2297
BseDI CCNNGG 3 cut(s) 383, 846, 2582
BseJI GATNNNNATC 4 cut(s) 62, 1623, 1818, 2517
BseMI GCAATG 4 cut(s) 2211, 2244, 2488, 2802
BseMII CTCAG 9 cut(s) 297, 579, 596, 795, 1629, 2001, 2277, 2478, 3122
BseNI ACTGG 4 cut(s) 306, 885, 1401, 1767
BseXI GCAGC 5 cut(s) 452, 2198, 2246, 2320, 2432
BsgI GTGCAG 1 cut(s) 2431
BshFI GGCC 7 cut(s) 156, 219, 809, 884, 1399, 2405, 2814
BshTI ACCGGT 3 cut(s) 1105, 1243, 1381
BsiHKAI GWGCWC 3 cut(s) 874, 1045, 2325
BsiHKCI CYCGRG 1 cut(s) 2548
BsiSI CCGG 5 cut(s) 207, 968, 1106, 1244, 1382
BslFI GGGAC 2 cut(s) 1708, 2565
BsmBI CGTCTC 1 cut(s) 1361
BsmFI GGGAC 2 cut(s) 1708, 2565
BsmI GAATGC 1 cut(s) 2004
BsnI GGCC 7 cut(s) 156, 219, 809, 884, 1399, 2405, 2814
Bso31I GGTCTC 1 cut(s) 1690
BsoBI CYCGRG 1 cut(s) 2548
Bsp1286I GDGCHC 4 cut(s) 207, 874, 1045, 2325
Bsp1720I GCTNAGC 1 cut(s) 1830
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 5 cut(s) 414, 468, 720, 993, 1660
BspANI GGCC 7 cut(s) 156, 219, 809, 884, 1399, 2405, 2814
BspCNI CTCAG 9 cut(s) 298, 580, 595, 796, 1630, 2002, 2278, 2479, 3123
BspHI TCATGA 4 cut(s) 1561, 1711, 1744, 2521
BspLI GGNNCC 2 cut(s) 204, 966
BspOI GCTAGC 1 cut(s) 2622
BspPI GGATC 5 cut(s) 85, 615, 625, 1775, 2872
BspTI CTTAAG 1 cut(s) 3070
BspTNI GGTCTC 1 cut(s) 1690
BsrDI GCAATG 4 cut(s) 2211, 2244, 2488, 2802
BsrFI RCCGGY 3 cut(s) 1105, 1243, 1381
BsrI ACTGG 4 cut(s) 306, 885, 1401, 1767
BssAI RCCGGY 3 cut(s) 1105, 1243, 1381
BssECI CCNNGG 3 cut(s) 383, 846, 2582
BssSI CACGAG 1 cut(s) 906
BssT1I CCWWGG 2 cut(s) 846, 2582
Bst2BI CACGAG 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 2297
Bst4CI ACNGT 5 cut(s) 224, 857, 1127, 1574, 3059
BstAFI CTTAAG 1 cut(s) 3070
BstC8I GCNNGC 3 cut(s) 1374, 1518, 2620
BstDSI CCRYGG 2 cut(s) 383, 846
BstHHI GCGC 1 cut(s) 3004
BstMWI GCNNNNNNNGC 4 cut(s) 474, 1550, 2186, 3032
BstNI CCWGG 1 cut(s) 2297
BstNSI RCATGY 3 cut(s) 765, 1084, 3008
BstSCI CCNGG 2 cut(s) 205, 2295
BstSFI CTRYAG 1 cut(s) 1968
BstV1I GCAGC 5 cut(s) 452, 2198, 2246, 2320, 2432
BstV2I GAAGAC 3 cut(s) 828, 1041, 2922
BstX2I RGATCY 3 cut(s) 235, 1780, 3094
BstXI CCANNNNNNTGG 1 cut(s) 2774
BstYI RGATCY 3 cut(s) 235, 1780, 3094
BsuRI GGCC 7 cut(s) 156, 219, 809, 884, 1399, 2405, 2814
BtgI CCRYGG 2 cut(s) 383, 846
BtgZI GCGATG 2 cut(s) 321, 432
BtsI GCAGTG 2 cut(s) 2598, 2794
BtsIMutI CAGTG 4 cut(s) 1774, 2598, 2794, 3055
Cac8I GCNNGC 3 cut(s) 1374, 1518, 2620
CaiI CAGNNNCTG 3 cut(s) 301, 928, 1466
CciI TCATGA 4 cut(s) 1561, 1711, 1744, 2521
CfoI GCGC 1 cut(s) 3004
Cfr10I RCCGGY 3 cut(s) 1105, 1243, 1381
Cfr13I GGNCC 4 cut(s) 217, 1398, 2389, 2471
CseI GACGC 1 cut(s) 1378
Csp6I GTAC 5 cut(s) 370, 493, 1536, 2260, 2290
CspAI ACCGGT 3 cut(s) 1105, 1243, 1381
CviQI GTAC 5 cut(s) 370, 493, 1536, 2260, 2290
DraI TTTAAA 1 cut(s) 2136
DraIII CACNNNGTG 1 cut(s) 1049
EaeI YGGCCR 1 cut(s) 2812
Ecl136II GAGCTC 2 cut(s) 872, 2323
Eco130I CCWWGG 2 cut(s) 846, 2582
Eco24I GRGCYC 3 cut(s) 207, 874, 2325
Eco31I GGTCTC 1 cut(s) 1690
Eco47I GGWCC 2 cut(s) 2389, 2471
Eco53kI GAGCTC 2 cut(s) 872, 2323
Eco57I CTGAAG 2 cut(s) 1542, 2928
Eco88I CYCGRG 1 cut(s) 2548
EcoICRI GAGCTC 2 cut(s) 872, 2323
EcoRI GAATTC 3 cut(s) 691, 1853, 3157
EcoRII CCWGG 1 cut(s) 2295
EcoT14I CCWWGG 2 cut(s) 846, 2582
EcoT22I ATGCAT 3 cut(s) 1086, 1224, 1362
EcoT38I GRGCYC 3 cut(s) 207, 874, 2325
ErhI CCWWGG 2 cut(s) 846, 2582
Esp3I CGTCTC 1 cut(s) 1361
FalI AAGNNNNNCTT 4 cut(s) 2446, 2478, 2661, 2693
FaqI GGGAC 2 cut(s) 1708, 2565
FauNDI CATATG 1 cut(s) 1656
FbaI TGATCA 4 cut(s) 598, 943, 1708, 2734
Fnu4HI GCNGC 8 cut(s) 6, 415, 466, 993, 2187, 2235, 2309, 2446
FriOI GRGCYC 3 cut(s) 207, 874, 2325
Fsp4HI GCNGC 8 cut(s) 6, 415, 466, 993, 2187, 2235, 2309, 2446
FspBI CTAG 7 cut(s) 183, 1533, 1850, 2369, 2565, 2619, 2648
FspI TGCGCA 1 cut(s) 3003
GlaI GCGC 1 cut(s) 3003
GluI GCNGC 8 cut(s) 6, 415, 466, 993, 2187, 2235, 2309, 2446
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 7 cut(s) 156, 219, 809, 884, 1399, 2405, 2814
HapII CCGG 5 cut(s) 207, 968, 1106, 1244, 1382
HgaI GACGC 1 cut(s) 1378
HhaI GCGC 1 cut(s) 3004
Hin6I GCGC 1 cut(s) 3002
HinP1I GCGC 1 cut(s) 3002
HindIII AAGCTT 3 cut(s) 2130, 2250, 2675
HpaII CCGG 5 cut(s) 207, 968, 1106, 1244, 1382
HphI GGTGA 7 cut(s) 284, 608, 869, 1004, 2087, 2789, 3092
HpyAV CCTTC 8 cut(s) 113, 528, 587, 1794, 1936, 2010, 2380, 2524
HpyCH4III ACNGT 5 cut(s) 224, 857, 1127, 1574, 3059
HpyF10VI GCNNNNNNNGC 4 cut(s) 474, 1550, 2186, 3032
HspAI GCGC 1 cut(s) 3002
Ksp22I TGATCA 4 cut(s) 598, 943, 1708, 2734
LmnI GCTCC 3 cut(s) 202, 2365, 3071
Lsp1109I GCAGC 5 cut(s) 452, 2198, 2246, 2320, 2432
LweI GCATC 7 cut(s) 568, 727, 928, 934, 2045, 2578, 2969
MaeI CTAG 7 cut(s) 183, 1533, 1850, 2369, 2565, 2619, 2648
MaeIII GTNAC 5 cut(s) 27, 776, 1010, 3080, 3125
MfeI CAATTG 1 cut(s) 2730
MflI RGATCY 3 cut(s) 235, 1780, 3094
MhlI GDGCHC 4 cut(s) 207, 874, 1045, 2325
MlsI TGGCCA 1 cut(s) 2814
MluNI TGGCCA 1 cut(s) 2814
MlyI GAGTC 5 cut(s) 317, 1190, 1328, 1463, 2091
MmeI TCCRAC 1 cut(s) 585
Mox20I TGGCCA 1 cut(s) 2814
Mph1103I ATGCAT 3 cut(s) 1086, 1224, 1362
MroXI GAANNNNTTC 3 cut(s) 84, 1183, 1845
MscI TGGCCA 1 cut(s) 2814
MseI TTAA 7 cut(s) 444, 1305, 1440, 2135, 2315, 2871, 3071
MslI CAYNNNNRTG 6 cut(s) 258, 1566, 1655, 2772, 3009, 3123
Msp20I TGGCCA 1 cut(s) 2814
MspCI CTTAAG 1 cut(s) 3070
MspI CCGG 5 cut(s) 207, 968, 1106, 1244, 1382
MspR9I CCNGG 2 cut(s) 207, 2297
MunI CAATTG 1 cut(s) 2730
Mva1269I GAATGC 1 cut(s) 2004
MvaI CCWGG 1 cut(s) 2297
MwoI GCNNNNNNNGC 4 cut(s) 474, 1550, 2186, 3032
NciI CCSGG 1 cut(s) 207
NcoI CCATGG 1 cut(s) 846
NdeI CATATG 1 cut(s) 1656
NheI GCTAGC 1 cut(s) 2618
NlaIV GGNNCC 2 cut(s) 204, 966
NmuCI GTSAC 4 cut(s) 776, 1010, 3080, 3125
NsbI TGCGCA 1 cut(s) 3003
NsiI ATGCAT 3 cut(s) 1086, 1224, 1362
NspI RCATGY 3 cut(s) 765, 1084, 3008
PagI TCATGA 4 cut(s) 1561, 1711, 1744, 2521
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 2004
PdmI GAANNNNTTC 3 cut(s) 84, 1183, 1845
PflFI GACNNNGTC 1 cut(s) 782
PflMI CCANNNNNTGG 2 cut(s) 301, 2200
PinAI ACCGGT 3 cut(s) 1105, 1243, 1381
PkrI GCNGC 8 cut(s) 7, 416, 467, 994, 2188, 2236, 2310, 2447
PleI GAGTC 5 cut(s) 316, 1190, 1328, 1463, 2090
PpsI GAGTC 5 cut(s) 316, 1190, 1328, 1463, 2090
PsiI TTATAA 1 cut(s) 1023
Psp124BI GAGCTC 2 cut(s) 874, 2325
Psp6I CCWGG 1 cut(s) 2295
PspGI CCWGG 1 cut(s) 2295
PspN4I GGNNCC 2 cut(s) 204, 966
PspPI GGNCC 4 cut(s) 217, 1398, 2389, 2471
PstNI CAGNNNCTG 3 cut(s) 301, 928, 1466
PsuI RGATCY 3 cut(s) 235, 1780, 3094
PsyI GACNNNGTC 1 cut(s) 782
RsaI GTAC 5 cut(s) 371, 494, 1537, 2261, 2291
RsaNI GTAC 5 cut(s) 370, 493, 1536, 2260, 2290
RseI CAYNNNNRTG 6 cut(s) 258, 1566, 1655, 2772, 3009, 3123
SacI GAGCTC 2 cut(s) 874, 2325
SaqAI TTAA 7 cut(s) 444, 1305, 1440, 2135, 2315, 2871, 3071
SatI GCNGC 8 cut(s) 6, 415, 466, 993, 2187, 2235, 2309, 2446
Sau96I GGNCC 4 cut(s) 217, 1398, 2389, 2471
ScaI AGTACT 1 cut(s) 1537
SchI GAGTC 5 cut(s) 317, 1190, 1328, 1463, 2091
ScrFI CCNGG 2 cut(s) 207, 2297
SduI GDGCHC 4 cut(s) 207, 874, 1045, 2325
SfaNI GCATC 7 cut(s) 568, 727, 928, 934, 2045, 2578, 2969
SfcI CTRYAG 1 cut(s) 1968
SinI GGWCC 2 cut(s) 2389, 2471
SmiMI CAYNNNNRTG 6 cut(s) 258, 1566, 1655, 2772, 3009, 3123
SmlI CTYRAG 8 cut(s) 312, 351, 478, 512, 1056, 1539, 2657, 3070
SmoI CTYRAG 8 cut(s) 312, 351, 478, 512, 1056, 1539, 2657, 3070
SpeI ACTAGT 1 cut(s) 1532
SsiI CCGC 5 cut(s) 414, 468, 720, 993, 1660
SspI AATATT 1 cut(s) 347
SspMI CTAG 7 cut(s) 183, 1533, 1850, 2369, 2565, 2619, 2648
SstI GAGCTC 2 cut(s) 874, 2325
StyD4I CCNGG 2 cut(s) 205, 2295
StyI CCWWGG 2 cut(s) 846, 2582
TaaI ACNGT 5 cut(s) 224, 857, 1127, 1574, 3059
TaqII GACCGA 1 cut(s) 1053
TatI WGTACW 1 cut(s) 1535
TauI GCSGC 2 cut(s) 417, 995
Tru1I TTAA 7 cut(s) 444, 1305, 1440, 2135, 2315, 2871, 3071
Tru9I TTAA 7 cut(s) 444, 1305, 1440, 2135, 2315, 2871, 3071
TscAI CASTG 4 cut(s) 1774, 2598, 2794, 3062
TseFI GTSAC 4 cut(s) 776, 1010, 3080, 3125
TseI GCWGC 6 cut(s) 5, 465, 2186, 2234, 2308, 2445
Tsp45I GTSAC 4 cut(s) 776, 1010, 3080, 3125
TspGWI ACGGA 3 cut(s) 372, 812, 2140
TspRI CASTG 4 cut(s) 1774, 2598, 2794, 3062
Tth111I GACNNNGTC 1 cut(s) 782
Van91I CCANNNNNTGG 2 cut(s) 301, 2200
Vha464I CTTAAG 1 cut(s) 3070
VpaK11BI GGWCC 2 cut(s) 2389, 2471
XapI RAATTY 5 cut(s) 691, 876, 1853, 3108, 3157
XbaI TCTAGA 2 cut(s) 1849, 2647
XceI RCATGY 3 cut(s) 765, 1084, 3008
XcmI CCANNNNNNNNNTGG 1 cut(s) 2197
XmnI GAANNNNTTC 3 cut(s) 84, 1183, 1845
XspI CTAG 7 cut(s) 183, 1533, 1850, 2369, 2565, 2619, 2648
ZrmI AGTACT 1 cut(s) 1537
Zsp2I ATGCAT 3 cut(s) 1086, 1224, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.