Rh4BG415600

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011757
Physical Location & Seq
Reverse (-)
56357537 .. 56362947
5411 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG415600.1

Sequence Viewer

Length: 3171 bp
ATGGCTGCCAACAAGTTTGCCACCATGTTACACAGAAACACCAACAAACTCACTCTGATTCTCATCTACGCAATCCTCGAATGGGTTCTGATCCTCCTCCTCCTTCTCAACTCCCTCTTCTCTTATCTGATCGTCAAGTTCGCCGATTACTTTGGCCTCAAACGACCCTGCCTCTGGTGTTCTAGGCTCGATCACATCTTCGAGCCCGGAAACAAGAGCCACAGTTCTTACAGAGATCTTGTGTGTGAGGAACACGCCAATGAAATAGCCAAACTTGGGTTTTGCTCGAATCACCAGAAACTGGCTGAGTCTCAAGATATGTGTGAGGATTGCTCATCGCAGGAATATTCTCAAGAATGGTCTAACAAGTACGCTTTCTTTCCGTGGATGAAGCAAATTGGGATGATTCAAGGCGGCGATGATGATAAGGAGATTAACGATAAGGAATGTTCTTGCTGCGGTGTGAGCTTGAGCAACAAGCTGTACCCTCCTTGTATTCTGCTCAAGCCTTCTTGGGGGGTTTTGGATTACGCTCAAAAACCGATTTTGAGTTCAGATGTAGGGGTTGATGCTCAGACTGAGGAAGGTGATCAGTCGGATCAAAGTGGATCGGATTATGTGGCAGATCAAAATGGACATGATGAGGGTTTTGTTGTTGTTGTTCAAGGAAATAGGGGAGAGAATTCGGTTTCGGATGTTGATGGATGTTGTGGACAAAGAGACTATGATACAGAGGAGAAGAATAATTCAGCATGTTGTTCTGTTTGTGATTATGTCTGCAAGGAAACGGAAACTGAGGCCAATCAAATTGAAGACGAGGCTAATATGAATGTCTCCATGGATGACGGTGATGATGGTCGGGCTCAAATTCAGGCCAGTTGTGATGAGAATAAGTCTCGTGGAAGTCTGCATCAGCATCTGGAGTTCTACATTGATCAAGATGATTGTCGGTTGGTTCCGGTTGAATTGGTTGATTCTCCTGCCACCATTGAGAGGCAAAGTCACCAGAATTATAAGGTGGACGAAGACCGAGCACATAGTGATACTCAAGATGTTATTCTGGATTTCGACATGCATTTCGAGACACAAGCCAAACCGGTTGTTGAGTTGTGGTACAGTTCAGATGAAATTGTGGAATTACTTTCATCCCATGAGAGTAAAGAGGAGAGCAAAGTTTCAGTTCTTGACTCAAAGGGTTTGGATTTCGATATGCATTTCAAGACACAAGCCAAACCGGCTGTTGAGTGTTGGCAGAGTTCAGATGAGATTGTGGAATTACTTTCATCCCATGAGATTAAAGAGGAGAGCGAAGTTTCAGTTCTTGACTCAAAGGATTTGGATTTCGATATGCATTTTGAGACGCAAGCCAAACCGGTTGTTGAGTGTTGGCCCAGTTCAGATGAGATTGTGGTACTTTCATCCCATGAGATTAAAAAGGAGAACAAAGTTTCAGTTCTTGACTCAAAGGATTTGGGGCAGAATGGAGAAGAAGTTGAAGAAAATGAGCAAGCTGAAGATACTACTAGAACTCAAGCAACACCAGCCTCTTGTCATGAGGATGACAGTCAATCAAATGCTGCTGATATAGTAGGAAGAGACATTGATTCAGATGTTCATCAAGCAACAACTGAGGATGATGACATCCATAAGGGGAACGATGAGATTGATACAGAGGTCTCAATAGGGACTGATATTCCTGATCATGATATATTCAATGAGGATATTCAATCAGCTCATGAACTTCTACATTCTTATCCCAGTGTGCAAGAAGATCCTTCTACAAGTTCTCCCAATGCTGAAATTGATGATAATTATGGTTCTAAGCAAGAAGAAGAGTTTCTAGAATTCAAGACCTTATCAGTTGAAACAAGTGAGAAAGAACAAAATAGTTATTTCTCATTAGGTTCAGAACTTAATGAGATTGAGGAAGAGAAGGTTCTTCCTGATACACCAACTTCTATAGACAATTTCCATCAGTTGTATAATAGAATGCTACTAACTGAGAGAAGGGAATCGGGAACAGAAGCATCATTGGATGGAAGTGTAGTGAGCGACATTGAATGTGGTGATGGAGTCCTGACTATGGAGAAGCTGAAATCGGTGTTGAGAGCTGAACGGAAAGCTTTAAATAGAGTATATGCAGAACTCGAAGAAGAGAGGAGTGCTTCTGCAATAGCAGCCAACCAGACAATGGCAATGATAAATAGGCTACAAGAAGAAAAGGCAGCAATGCAGATGGAAGCTTTGCAGTACCAAAGAATGATGGATGAGCAGTCTGAGTACGACCAGGAAGCTATGCAGCTCTTAAACGAGCTCATGGTAAAGAGGGAAAAGGAAAAGCAAGAACTTGAAAAGGAGCTGGAAATATGTCGAAAGAAGATCCAAGATTATGAGGCCAAAGAGAAAATGATGATTTTGAGGAGAATGAAAGAGGGCTGCACAAGAAGTAGGACTTCATCTGGTCCTTGTAGCAATGCTGAGGATAGTGATGGACTTTCTATTGATTTGAATCATGAAGTGAAGGAAGAAGATAACCTCTCGGGACATGAAGGGAGTAGCAACCAAAACACGCCAAGGGATGCGGTGTTATATTTAGAAGAATCTTTGGCTAGCTTTGAGGAAGAGAAGTTATCCATTCTAGACCAACTCAAGGAATTGGAAGAGAAGCTTTTGACCTTGAATGACGAAGAGGAAGAAGAACATTACGAGGATATAAAACCAATTGATCATTTCTTTTCAGAGAATGGGAATGGCTACCATGAGAATGGTGATGTTAGCAGTGAAGAAAATGGCATTGCAAATGGCCACTCCAAAGAAATGAATGGAACACATCATCAAGATAGAAAAATTATGGGATCAATCAAGGCAAAGAGACTTCTCCCACTTTTCAATGAAGCTGAAGATGAAGACGAGGAGTTGAATGGAGATGTAGAAGGTTATGATTCTGTTGCGTCACAAGATCACAAGAAACTCGCTATTGAAGAGGCGGTTGATCATGTCTATGAGAGACTACAAGCACTTGAAGCAGATAGAGAATTTCTAAAGCACTGTATTAGCTCCTTAAGGAAAGGTGACAAGGGATTAGATCTTCTCCAAGAAATTTTGCAACATCTTCGTGACCTGAGAAGTGTGGAAACCCGTCTAAGGAATTCGGGAGAGGGTATCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1056

Amino Acids

119.69

Weight (kDa)

4.43

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 697 - 787 1.1e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1014
AccB7I CCANNNNNTGG 2 cut(s) 301, 2191
AciI CCGC 4 cut(s) 414, 459, 2582, 2987
AclWI GGATC 6 cut(s) 85, 606, 616, 1766, 2374, 2863
AcoI YGGCCR 1 cut(s) 2803
AcsI RAATTY 6 cut(s) 682, 867, 1844, 3035, 3099, 3148
AcuI CTGAAG 2 cut(s) 1533, 2919
AdeI CACNNNGTG 1 cut(s) 1040
AfaI GTAC 6 cut(s) 371, 485, 1115, 1413, 2252, 2282
AflII CTTAAG 1 cut(s) 3061
AgeI ACCGGT 2 cut(s) 1096, 1372
AjnI CCWGG 1 cut(s) 2286
Alw21I GWGCWC 2 cut(s) 1036, 2316
AlwI GGATC 6 cut(s) 85, 606, 616, 1766, 2374, 2863
AlwNI CAGNNNCTG 2 cut(s) 301, 919
Ama87I CYCGRG 1 cut(s) 2539
AoxI GGCC 6 cut(s) 154, 798, 873, 1388, 2394, 2803
ApeKI GCWGC 7 cut(s) 5, 456, 1577, 2177, 2225, 2299, 2436
ApoI RAATTY 6 cut(s) 682, 867, 1844, 3035, 3099, 3148
ArsI GACNNNNNNTTYG 2 cut(s) 1061, 1093
AsiGI ACCGGT 2 cut(s) 1096, 1372
Asp700I GAANNNNTTC 2 cut(s) 84, 1836
AspS9I GGNCC 2 cut(s) 1389, 2462
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 7 cut(s) 284, 599, 860, 995, 2078, 2780, 3083
AsuNHI GCTAGC 1 cut(s) 2609
AvaI CYCGRG 1 cut(s) 2539
AvaII GGWCC 1 cut(s) 2462
BaeI ACNNNNGTAYC 2 cut(s) 1659, 1692
BalI TGGCCA 1 cut(s) 2805
BanII GRGCYC 3 cut(s) 207, 865, 2316
BauI CACGAG 1 cut(s) 897
BbsI GAAGAC 3 cut(s) 819, 1032, 2913
Bbv12I GWGCWC 2 cut(s) 1036, 2316
BbvCI CCTCAGC 1 cut(s) 2478
BbvI GCAGC 6 cut(s) 443, 1564, 2189, 2237, 2311, 2423
BccI CCATC 8 cut(s) 695, 848, 1980, 2030, 2063, 2230, 2257, 2483
BcgI CGANNNNNNTGC 2 cut(s) 3095, 3129
BciT130I CCWGG 1 cut(s) 2288
BclI TGATCA 5 cut(s) 589, 934, 1699, 2725, 2992
BcnI CCSGG 1 cut(s) 207
BfaI CTAG 5 cut(s) 183, 1524, 1841, 2610, 2639
BfmI CTRYAG 1 cut(s) 1959
BfrI CTTAAG 1 cut(s) 3061
BglI GCCNNNNNGGC 1 cut(s) 1235
BglII AGATCT 2 cut(s) 235, 3085
BisI GCNGC 8 cut(s) 6, 415, 457, 1578, 2178, 2226, 2300, 2437
BlsI GCNGC 8 cut(s) 7, 416, 458, 1579, 2179, 2227, 2301, 2438
Bme1390I CCNGG 2 cut(s) 207, 2288
Bme18I GGWCC 1 cut(s) 2462
BmeT110I CYCGRG 1 cut(s) 2539
BmgT120I GGNCC 2 cut(s) 1389, 2462
BmiI GGNNCC 1 cut(s) 957
BmrFI CCNGG 2 cut(s) 207, 2288
BmrI ACTGGG 2 cut(s) 1386, 1752
BmsI GCATC 5 cut(s) 559, 919, 925, 2036, 2569
BmtI GCTAGC 1 cut(s) 2613
BmuI ACTGGG 2 cut(s) 1386, 1752
BpiI GAAGAC 3 cut(s) 819, 1032, 2913
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 941
Bpu10I CCTNAGC 1 cut(s) 2478
BpuEI CTTGAG 7 cut(s) 297, 336, 488, 490, 1032, 1515, 2633
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 3 cut(s) 62, 1614, 2508
BsaI GGTCTC 1 cut(s) 1681
BsaJI CCNNGG 3 cut(s) 383, 837, 2573
BsaWI WCCGGW 3 cut(s) 958, 1096, 1372
BsaXI ACNNNNNCTCC 4 cut(s) 1476, 1506, 1771, 1801
Bse118I RCCGGY 3 cut(s) 1096, 1234, 1372
Bse1I ACTGG 4 cut(s) 306, 876, 1392, 1758
Bse3DI GCAATG 4 cut(s) 2202, 2235, 2479, 2793
Bse8I GATNNNNATC 3 cut(s) 62, 1614, 2508
BseBI CCWGG 1 cut(s) 2288
BseDI CCNNGG 3 cut(s) 383, 837, 2573
BseJI GATNNNNATC 3 cut(s) 62, 1614, 2508
BseMI GCAATG 4 cut(s) 2202, 2235, 2479, 2793
BseMII CTCAG 9 cut(s) 297, 570, 587, 786, 1620, 1992, 2268, 2469, 3113
BseNI ACTGG 4 cut(s) 306, 876, 1392, 1758
BseRI GAGGAG 8 cut(s) 86, 89, 749, 1178, 1316, 2173, 2434, 2927
BseXI GCAGC 6 cut(s) 443, 1564, 2189, 2237, 2311, 2423
BsgI GTGCAG 1 cut(s) 2422
BshFI GGCC 6 cut(s) 156, 800, 875, 1390, 2396, 2805
BshTI ACCGGT 2 cut(s) 1096, 1372
BsiHKAI GWGCWC 2 cut(s) 1036, 2316
BsiHKCI CYCGRG 1 cut(s) 2539
BsiSI CCGG 5 cut(s) 207, 959, 1097, 1235, 1373
BslFI GGGAC 2 cut(s) 1699, 2556
BsmBI CGTCTC 1 cut(s) 1352
BsmFI GGGAC 2 cut(s) 1699, 2556
BsmI GAATGC 1 cut(s) 1995
BsnI GGCC 6 cut(s) 156, 800, 875, 1390, 2396, 2805
Bso31I GGTCTC 1 cut(s) 1681
BsoBI CYCGRG 1 cut(s) 2539
Bsp1286I GDGCHC 4 cut(s) 207, 865, 1036, 2316
Bsp19I CCATGG 1 cut(s) 837
BspACI CCGC 4 cut(s) 414, 459, 2582, 2987
BspANI GGCC 6 cut(s) 156, 800, 875, 1390, 2396, 2805
BspCNI CTCAG 9 cut(s) 298, 571, 586, 787, 1621, 1993, 2269, 2470, 3114
BspHI TCATGA 4 cut(s) 1552, 1702, 1735, 2512
BspLI GGNNCC 1 cut(s) 957
BspOI GCTAGC 1 cut(s) 2613
BspPI GGATC 6 cut(s) 85, 606, 616, 1766, 2374, 2863
BspTI CTTAAG 1 cut(s) 3061
BspTNI GGTCTC 1 cut(s) 1681
BsrDI GCAATG 4 cut(s) 2202, 2235, 2479, 2793
BsrFI RCCGGY 3 cut(s) 1096, 1234, 1372
BsrI ACTGG 4 cut(s) 306, 876, 1392, 1758
BssAI RCCGGY 3 cut(s) 1096, 1234, 1372
BssECI CCNNGG 3 cut(s) 383, 837, 2573
BssSI CACGAG 1 cut(s) 897
BssT1I CCWWGG 2 cut(s) 837, 2573
Bst2BI CACGAG 1 cut(s) 897
Bst2UI CCWGG 1 cut(s) 2288
Bst4CI ACNGT 5 cut(s) 224, 848, 1118, 1565, 3050
Bst6I CTCTTC 9 cut(s) 122, 1588, 1827, 1923, 2148, 2616, 2655, 2682, 2976
BstAFI CTTAAG 1 cut(s) 3061
BstC8I GCNNGC 3 cut(s) 1365, 1509, 2611
BstDSI CCRYGG 2 cut(s) 383, 837
BstMWI GCNNNNNNNGC 5 cut(s) 465, 1235, 1541, 2177, 3023
BstNI CCWGG 1 cut(s) 2288
BstNSI RCATGY 2 cut(s) 756, 1075
BstSCI CCNGG 2 cut(s) 205, 2286
BstSFI CTRYAG 1 cut(s) 1959
BstV1I GCAGC 6 cut(s) 443, 1564, 2189, 2237, 2311, 2423
BstV2I GAAGAC 3 cut(s) 819, 1032, 2913
BstX2I RGATCY 4 cut(s) 235, 1771, 2379, 3085
BstXI CCANNNNNNTGG 1 cut(s) 2765
BstYI RGATCY 4 cut(s) 235, 1771, 2379, 3085
BsuRI GGCC 6 cut(s) 156, 800, 875, 1390, 2396, 2805
BtgI CCRYGG 2 cut(s) 383, 837
BtgZI GCGATG 2 cut(s) 321, 432
BtsI GCAGTG 1 cut(s) 2785
BtsIMutI CAGTG 3 cut(s) 1765, 2785, 3046
Cac8I GCNNGC 3 cut(s) 1365, 1509, 2611
CaiI CAGNNNCTG 2 cut(s) 301, 919
CciI TCATGA 4 cut(s) 1552, 1702, 1735, 2512
Cfr10I RCCGGY 3 cut(s) 1096, 1234, 1372
Cfr13I GGNCC 2 cut(s) 1389, 2462
CseI GACGC 2 cut(s) 1369, 2940
Csp6I GTAC 6 cut(s) 370, 484, 1114, 1412, 2251, 2281
CspAI ACCGGT 2 cut(s) 1096, 1372
CviQI GTAC 6 cut(s) 370, 484, 1114, 1412, 2251, 2281
DraI TTTAAA 1 cut(s) 2127
DraIII CACNNNGTG 1 cut(s) 1040
EaeI YGGCCR 1 cut(s) 2803
Eam1104I CTCTTC 9 cut(s) 122, 1588, 1827, 1923, 2148, 2616, 2655, 2682, 2976
EarI CTCTTC 9 cut(s) 122, 1588, 1827, 1923, 2148, 2616, 2655, 2682, 2976
Ecl136II GAGCTC 1 cut(s) 2314
Eco130I CCWWGG 2 cut(s) 837, 2573
Eco24I GRGCYC 3 cut(s) 207, 865, 2316
Eco31I GGTCTC 1 cut(s) 1681
Eco47I GGWCC 1 cut(s) 2462
Eco53kI GAGCTC 1 cut(s) 2314
Eco57I CTGAAG 2 cut(s) 1533, 2919
Eco88I CYCGRG 1 cut(s) 2539
EcoICRI GAGCTC 1 cut(s) 2314
EcoRI GAATTC 3 cut(s) 682, 1844, 3148
EcoRII CCWGG 1 cut(s) 2286
EcoT14I CCWWGG 2 cut(s) 837, 2573
EcoT22I ATGCAT 3 cut(s) 1077, 1215, 1353
EcoT38I GRGCYC 3 cut(s) 207, 865, 2316
ErhI CCWWGG 2 cut(s) 837, 2573
Esp3I CGTCTC 1 cut(s) 1352
FalI AAGNNNNNCTT 4 cut(s) 2437, 2469, 2652, 2684
FaqI GGGAC 2 cut(s) 1699, 2556
FbaI TGATCA 5 cut(s) 589, 934, 1699, 2725, 2992
Fnu4HI GCNGC 8 cut(s) 6, 415, 457, 1578, 2178, 2226, 2300, 2437
FriOI GRGCYC 3 cut(s) 207, 865, 2316
Fsp4HI GCNGC 8 cut(s) 6, 415, 457, 1578, 2178, 2226, 2300, 2437
FspBI CTAG 5 cut(s) 183, 1524, 1841, 2610, 2639
GluI GCNGC 8 cut(s) 6, 415, 457, 1578, 2178, 2226, 2300, 2437
GsuI CTGGAG 1 cut(s) 941
HaeIII GGCC 6 cut(s) 156, 800, 875, 1390, 2396, 2805
HapII CCGG 5 cut(s) 207, 959, 1097, 1235, 1373
HgaI GACGC 2 cut(s) 1369, 2940
HindIII AAGCTT 3 cut(s) 2121, 2241, 2666
HpaII CCGG 5 cut(s) 207, 959, 1097, 1235, 1373
HphI GGTGA 7 cut(s) 284, 599, 860, 995, 2078, 2780, 3083
Hpy166II GTNNAC 2 cut(s) 713, 1021
Hpy8I GTNNAC 2 cut(s) 713, 1021
HpyAV CCTTC 9 cut(s) 113, 519, 578, 1785, 1927, 2001, 2515, 2543, 2927
HpyCH4III ACNGT 5 cut(s) 224, 848, 1118, 1565, 3050
HpyF10VI GCNNNNNNNGC 5 cut(s) 465, 1235, 1541, 2177, 3023
Ksp22I TGATCA 5 cut(s) 589, 934, 1699, 2725, 2992
LmnI GCTCC 2 cut(s) 2356, 3062
Lsp1109I GCAGC 6 cut(s) 443, 1564, 2189, 2237, 2311, 2423
LweI GCATC 5 cut(s) 559, 919, 925, 2036, 2569
MaeI CTAG 5 cut(s) 183, 1524, 1841, 2610, 2639
MaeIII GTNAC 5 cut(s) 27, 1001, 2952, 3071, 3116
MfeI CAATTG 1 cut(s) 2721
MflI RGATCY 4 cut(s) 235, 1771, 2379, 3085
MhlI GDGCHC 4 cut(s) 207, 865, 1036, 2316
MlsI TGGCCA 1 cut(s) 2805
MluNI TGGCCA 1 cut(s) 2805
MlyI GAGTC 5 cut(s) 317, 1181, 1319, 1454, 2082
MmeI TCCRAC 1 cut(s) 576
Mox20I TGGCCA 1 cut(s) 2805
Mph1103I ATGCAT 3 cut(s) 1077, 1215, 1353
MroXI GAANNNNTTC 2 cut(s) 84, 1836
MscI TGGCCA 1 cut(s) 2805
MseI TTAA 7 cut(s) 435, 1296, 1431, 1914, 2126, 2306, 3062
MslI CAYNNNNRTG 5 cut(s) 258, 1557, 2763, 3000, 3114
Msp20I TGGCCA 1 cut(s) 2805
MspCI CTTAAG 1 cut(s) 3061
MspI CCGG 5 cut(s) 207, 959, 1097, 1235, 1373
MspR9I CCNGG 2 cut(s) 207, 2288
MunI CAATTG 1 cut(s) 2721
Mva1269I GAATGC 1 cut(s) 1995
MvaI CCWGG 1 cut(s) 2288
MwoI GCNNNNNNNGC 5 cut(s) 465, 1235, 1541, 2177, 3023
NciI CCSGG 1 cut(s) 207
NcoI CCATGG 1 cut(s) 837
NheI GCTAGC 1 cut(s) 2609
NlaIV GGNNCC 1 cut(s) 957
NmuCI GTSAC 4 cut(s) 1001, 2952, 3071, 3116
NsiI ATGCAT 3 cut(s) 1077, 1215, 1353
NspI RCATGY 2 cut(s) 756, 1075
PagI TCATGA 4 cut(s) 1552, 1702, 1735, 2512
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 1995
PdmI GAANNNNTTC 2 cut(s) 84, 1836
PfeI GAWTC 9 cut(s) 58, 289, 406, 974, 1604, 2012, 2509, 2600, 2942
PflMI CCANNNNNTGG 2 cut(s) 301, 2191
PinAI ACCGGT 2 cut(s) 1096, 1372
PkrI GCNGC 8 cut(s) 7, 416, 458, 1579, 2179, 2227, 2301, 2438
PleI GAGTC 5 cut(s) 316, 1181, 1319, 1454, 2081
PpsI GAGTC 5 cut(s) 316, 1181, 1319, 1454, 2081
PsiI TTATAA 1 cut(s) 1014
Psp124BI GAGCTC 1 cut(s) 2316
Psp6I CCWGG 1 cut(s) 2286
PspGI CCWGG 1 cut(s) 2286
PspN4I GGNNCC 1 cut(s) 957
PspPI GGNCC 2 cut(s) 1389, 2462
PstNI CAGNNNCTG 2 cut(s) 301, 919
PsuI RGATCY 4 cut(s) 235, 1771, 2379, 3085
RsaI GTAC 6 cut(s) 371, 485, 1115, 1413, 2252, 2282
RsaNI GTAC 6 cut(s) 370, 484, 1114, 1412, 2251, 2281
RseI CAYNNNNRTG 5 cut(s) 258, 1557, 2763, 3000, 3114
SacI GAGCTC 1 cut(s) 2316
SaqAI TTAA 7 cut(s) 435, 1296, 1431, 1914, 2126, 2306, 3062
SatI GCNGC 8 cut(s) 6, 415, 457, 1578, 2178, 2226, 2300, 2437
Sau96I GGNCC 2 cut(s) 1389, 2462
SchI GAGTC 5 cut(s) 317, 1181, 1319, 1454, 2082
ScrFI CCNGG 2 cut(s) 207, 2288
SduI GDGCHC 4 cut(s) 207, 865, 1036, 2316
SfaNI GCATC 5 cut(s) 559, 919, 925, 2036, 2569
SfcI CTRYAG 1 cut(s) 1959
SinI GGWCC 1 cut(s) 2462
SmiMI CAYNNNNRTG 5 cut(s) 258, 1557, 2763, 3000, 3114
SmlI CTYRAG 8 cut(s) 312, 351, 469, 503, 1047, 1530, 2648, 3061
SmoI CTYRAG 8 cut(s) 312, 351, 469, 503, 1047, 1530, 2648, 3061
SsiI CCGC 4 cut(s) 414, 459, 2582, 2987
SspI AATATT 1 cut(s) 347
SspMI CTAG 5 cut(s) 183, 1524, 1841, 2610, 2639
SstI GAGCTC 1 cut(s) 2316
StyD4I CCNGG 2 cut(s) 205, 2286
StyI CCWWGG 2 cut(s) 837, 2573
TaaI ACNGT 5 cut(s) 224, 848, 1118, 1565, 3050
TaqII GACCGA 1 cut(s) 1044
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 9 cut(s) 58, 289, 406, 974, 1604, 2012, 2509, 2600, 2942
Tru1I TTAA 7 cut(s) 435, 1296, 1431, 1914, 2126, 2306, 3062
Tru9I TTAA 7 cut(s) 435, 1296, 1431, 1914, 2126, 2306, 3062
TscAI CASTG 3 cut(s) 1765, 2785, 3053
TseFI GTSAC 4 cut(s) 1001, 2952, 3071, 3116
TseI GCWGC 7 cut(s) 5, 456, 1577, 2177, 2225, 2299, 2436
Tsp45I GTSAC 4 cut(s) 1001, 2952, 3071, 3116
TspGWI ACGGA 3 cut(s) 372, 803, 2131
TspRI CASTG 3 cut(s) 1765, 2785, 3053
Van91I CCANNNNNTGG 2 cut(s) 301, 2191
Vha464I CTTAAG 1 cut(s) 3061
VpaK11BI GGWCC 1 cut(s) 2462
XapI RAATTY 6 cut(s) 682, 867, 1844, 3035, 3099, 3148
XbaI TCTAGA 2 cut(s) 1840, 2638
XceI RCATGY 2 cut(s) 756, 1075
XcmI CCANNNNNNNNNTGG 1 cut(s) 2188
XmnI GAANNNNTTC 2 cut(s) 84, 1836
XspI CTAG 5 cut(s) 183, 1524, 1841, 2610, 2639
Zsp2I ATGCAT 3 cut(s) 1077, 1215, 1353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.