MD13G1021300.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011757
Physical Location & Seq
Reverse (-)
1544417 .. 1548625
4209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1021300.v1.1.491

Sequence Viewer

Length: 2724 bp
ATGGCTGCCAACAAGTTTGCAACCATGCTGCACAGAAACACCAACAAGATCACTCTCGTTCTAGTCTATGCTCTCCTCGAATGGACTCTGATCATCCTCCTCCTTCTCAATTCCCTCTTTTCTTACCTAATCATAAAGTTCTCCGATTACTTTGGCCTCAGAAGACCCTGCCTCTGGTGTTCTAGGCTCGAACATATCTTCGAGCCCAGAAACACCAAAAGTTCTTATAGAGATCTCGTCTGCGAATCTCATGCCAATGAGATATCCCATCTGGGTTATTGTTCAAATCACCAGAAACTTGTAGAATCGCAAGATATGTGCGAGGACTGCTCATCGAAGCCGGATTGCGAAGAATGGTCAAAGAAGTTTGCTTTTTTTCCATGGATGAAGCAAATTGGGATGATTCGGGATAGCGATGAAAAAGTCACTGACAATGGAGAGGAGAAGTTGAACTGTTCTTGCTGCGGATTGAGTTTGGACAAGTTTTACCCTTCTTGTATTGTGATTAAACCTTCTTGGGAGGTTTTGGATTATACTCAGAAACAAAATTTGAGTTTGGAAACGGGGGATGATCATCAGACGGAAGAAGGTGATCGGTCGGATCGAAGCGGATCGGATTTTGTGATCGGAGACGAAGAGGGAGTTGAAGAAAAGAAGGGAGAAAATAGGGGTTTTGAAGCAGAGGATCATTCTGCTACTTGTTCTGTATGTGACTATGGCTGCAAGGAGACGGAGATTGTAGCCATTGGAGATGAACAAGTGGTGACAGACATAGAAGAAGAACAAGAACCCACAAAAGATATTGTATCTCCAAAAATTCGACCTCAGGATTTGGAGTTCTACATTGATCAAGACGATTGCCGGTTGGTTCTGGTTGATTTGATTGATTCTCCCGCCACCATTGAAAACCAGAGTCATCAGAAGTACAAGAAGGTGGAAGAATACCAAGGAAATAGTAGCGGTTGTGAAGATGTTATACTGGATTTCGACATTGCGAAGCCGGTTGTGGAGAATTGGCGCGGTTCAGAAGAGTCTGTGGCATTGCTTTCGTTCGATGAGAGTAAGGAAGAGGGGAGTAAGGTTTCAGTTCTTGATTTGAAGGATTCGGATGAGACTGGCATCGCGAAGGAGGGAAATGAACAGGTTGGAGCTATAGCAAATGGTGAAATTGTTTCAGATGTTCATCTAGCAATCGAGGATGAAGTCCTTCCTGGCAACGATGAGATTGAGGCGGAGGTCGCAATAGGGACAGAGATTCCTGATCAGGAACAGAATGACGAAGTTCAATCGGCTCAAGAACTCTTGAATTCGCATCCGTGTGAGCAGGAAGACCCTTCTACAAGTTCTGCCATTCTACATGTTTGTGATCGTCATGGTTCTGAGCGACCAGAGGAAGAATTACTTGAATTCAAAACCTTGTCGGCTGAAACGAGTGAGAAAGAAAAAGAAAATCATTTCTCATATGAGGAAGATGATGTTCTTGACACACCTACTTCAATAGACAGTCTCCACCAGTTGCATAAAGGAATGCTACTATTCGAGAGACGAGAACTGGTAACAGAAGACTCATTGGATGGAAGTGTTATGAGTGACATTGAAGGCGGTGATGGAGTCGTGACAGTTGAGAAATTAAAGACGGCGTTGAAAACTGAACGGAAGGCTTTGAACGAATTATATGCCGAACTTGAAGAAGAGAGGAGTGCTTCTGCAGTGGCAGCCAGTCAGACAATGGCAATGATAAATAGGTTACAAGAAGAAAAGGCAGCAATGCAGATGGAAGCTTTGCAGTACCAGAGAATGATGGAAGAGCAGTCTGAGTACGACCAGGAAGCGATGCAGCTCTTGAACGAGCTTATGGTGAAGCGGGAAAGGGAAAAGCAAGAGCTTGAAAAGGAGCTGGAAGTATGTCGAAAGAAGCTGCAGGATTATGAGGCCAAAGAGAGAGCGATGATTTTGAGGAGACTGAAAGATGGAAGCGTGAAAAGTAGAACTTCATCCGGTGCTTGTAGCAATGCTGAGGATAGCGATGGACTATCTACTGATTTGAATAACGAATTGAAGGAAGAAGATTGCTTATACGGGAATGAAGAAAGTAGCAATCAAAACACCCCGGCAGATGCAGTTCTATATCTGGAGAAATCTTTGGCTATCTTTGAGGAAGAGAAGTTGTCCATTCTAGACCAGCTCAAGGAGTTGGAAGAGAAGCTTTTGACCTTGAGCGAAGAAGAGGAAGAAGACTGTTATGAGGATATAAACCCAATCGAGCATTTCTTTTCGGAGAATGGAAATGATTACCATGAAAGTTCAGATGTCAGAAGTGAAGTAAATGGCAATGGCATTGCAAATGGCCATTCCAAAGAAATGAATGGAAATCATGATCAAGATAGAAAAATCAAGGGCTTGAAAGCAAAGAGACTTCTTCCGCTATTTGATGCCATCGAAACGGAATCAAAAGACGGGGAATTGAATGGAAACACAGAAGGGTATGATTCTTTTACATCACAAGAGTCGGAGAGCAAGAAACTTGCTATCGAGGAGGAGGTTGATCATGTGTATGAGAGACTACAAGCACTTGAAGCAGATAGGGAGTTTCTAAAGCACTGCATTTCCTCCTTGAGGAAAGGAGATAAGGGATTACTTCTTCTCCAAGAAATTTTGGAACATCTTCGCGATCTGCGAAGTGTAGAACTCCGTCTGAGGACCACGGCTGACAGCGTTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

908

Amino Acids

102.93

Weight (kDa)

4.46

Isoelectric Point (pI)

52.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 543 - 633 2.1e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 1020, 1124, 2670
AciI CCGC 9 cut(s) 465, 609, 894, 960, 1020, 1232, 1602, 1864, 2423
AclWI GGATC 3 cut(s) 609, 619, 693
AcoI YGGCCR 1 cut(s) 2347
AcsI RAATTY 5 cut(s) 547, 816, 1306, 1406, 2652
AfaI GTAC 3 cut(s) 926, 1790, 1820
AfiI CCNNNNNNNGG 3 cut(s) 174, 2188, 2616
AflIII ACRYGT 1 cut(s) 1357
AjnI CCWGG 2 cut(s) 1210, 1824
AloI GAACNNNNNNTCC 2 cut(s) 821, 853
AluBI AGCT 9 cut(s) 1151, 1781, 1840, 1852, 1885, 1897, 1918, 2185, 2206
AluI AGCT 9 cut(s) 1151, 1781, 1840, 1852, 1885, 1897, 1918, 2185, 2206
Alw26I GTCTC 8 cut(s) 624, 722, 1106, 1511, 1537, 1954, 2407, 2554
AlwI GGATC 3 cut(s) 609, 619, 693
AoxI GGCC 3 cut(s) 154, 1932, 2347
ApeKI GCWGC 8 cut(s) 5, 28, 462, 720, 1715, 1763, 1837, 1918
ApoI RAATTY 5 cut(s) 547, 816, 1306, 1406, 2652
Asp700I GAANNNNTTC 2 cut(s) 1206, 2664
AspLEI GCGC 1 cut(s) 1020
AspS9I GGNCC 1 cut(s) 2700
AsuC2I CCSGG 1 cut(s) 2111
AsuHPI GGTGA 6 cut(s) 281, 602, 775, 1175, 1616, 1870
AvaII GGWCC 1 cut(s) 2700
AxyI CCTNAGG 1 cut(s) 825
BalI TGGCCA 1 cut(s) 2349
BanII GRGCYC 1 cut(s) 207
BarI GAAGNNNNNNTAC 2 cut(s) 960, 992
BbsI GAAGAC 4 cut(s) 169, 1335, 1569, 2241
BbvCI CCTCAGC 1 cut(s) 2016
BbvI GCAGC 7 cut(s) 15, 449, 707, 1727, 1775, 1849, 1905
BccI CCATC 8 cut(s) 276, 1568, 1601, 1768, 1795, 1964, 2021, 2444
BceAI ACGGC 2 cut(s) 1653, 2721
BcgI CGANNNNNNTGC 6 cut(s) 233, 267, 1029, 1063, 1658, 1692
BciT130I CCWGG 2 cut(s) 1212, 1826
BclI TGATCA 6 cut(s) 90, 571, 847, 1261, 2377, 2545
BcnI CCSGG 1 cut(s) 2111
BcoDI GTCTC 8 cut(s) 624, 722, 1106, 1511, 1537, 1954, 2407, 2554
BfaI CTAG 4 cut(s) 62, 183, 1187, 2177
BfmI CTRYAG 3 cut(s) 1152, 1707, 1919
BglII AGATCT 1 cut(s) 232
BisI GCNGC 8 cut(s) 6, 29, 463, 721, 1716, 1764, 1838, 1919
BlsI GCNGC 8 cut(s) 7, 30, 464, 722, 1717, 1765, 1839, 1920
Bme1390I CCNGG 3 cut(s) 1212, 1826, 2111
Bme18I GGWCC 1 cut(s) 2700
BmgT120I GGNCC 1 cut(s) 2700
BmrFI CCNGG 3 cut(s) 1212, 1826, 2111
BmsI GCATC 5 cut(s) 1128, 1321, 1824, 2107, 2422
BpiI GAAGAC 4 cut(s) 169, 1335, 1569, 2241
BplI GAGNNNNNCTC 2 cut(s) 314, 346
BpmI CTGGAG 1 cut(s) 2153
Bpu10I CCTNAGC 1 cut(s) 2016
BpuEI CTTGAG 4 cut(s) 1278, 2171, 2236, 2635
BpuMI CCSGG 1 cut(s) 2111
BsaBI GATNNNNATC 2 cut(s) 573, 1182
BsaJI CCNNGG 4 cut(s) 380, 946, 2109, 2703
BsaWI WCCGGW 1 cut(s) 1997
BsaXI ACNNNNNCTCC 2 cut(s) 2628, 2658
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 2188, 2616
Bse118I RCCGGY 2 cut(s) 861, 1000
Bse1I ACTGG 5 cut(s) 984, 1120, 1513, 1557, 1719
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 7 cut(s) 990, 1040, 1740, 1773, 2017, 2337, 2338
Bse8I GATNNNNATC 2 cut(s) 573, 1182
BseBI CCWGG 2 cut(s) 1212, 1826
BseDI CCNNGG 4 cut(s) 380, 946, 2109, 2703
BseGI GGATG 9 cut(s) 93, 390, 405, 574, 1114, 1204, 1312, 1579, 1994
BseJI GATNNNNATC 2 cut(s) 573, 1182
BseLI CCNNNNNNNGG 3 cut(s) 174, 2188, 2616
BseMI GCAATG 7 cut(s) 990, 1040, 1740, 1773, 2017, 2337, 2338
BseMII CTCAG 7 cut(s) 172, 551, 839, 1371, 1806, 2007, 2687
BseNI ACTGG 5 cut(s) 984, 1120, 1513, 1557, 1719
BseRI GAGGAG 7 cut(s) 65, 89, 455, 1711, 1972, 2549, 2552
BseXI GCAGC 7 cut(s) 15, 449, 707, 1727, 1775, 1849, 1905
BsgI GTGCAG 1 cut(s) 14
Bsh1236I CGCG 3 cut(s) 1020, 1124, 2670
Bsh1285I CGRYCG 1 cut(s) 599
BshFI GGCC 3 cut(s) 156, 1934, 2349
BsiEI CGRYCG 1 cut(s) 599
BsiSI CCGG 5 cut(s) 341, 862, 1001, 1998, 2111
BslFI GGGAC 1 cut(s) 1261
BslI CCNNNNNNNGG 3 cut(s) 174, 2188, 2616
BsmAI GTCTC 8 cut(s) 624, 722, 1106, 1511, 1537, 1954, 2407, 2554
BsmBI CGTCTC 3 cut(s) 624, 722, 1537
BsmFI GGGAC 1 cut(s) 1261
BsmI GAATGC 1 cut(s) 1533
BsnI GGCC 3 cut(s) 156, 1934, 2349
Bsp1286I GDGCHC 1 cut(s) 207
Bsp19I CCATGG 1 cut(s) 380
Bsp68I TCGCGA 2 cut(s) 1124, 2670
BspACI CCGC 9 cut(s) 465, 609, 894, 960, 1020, 1232, 1602, 1864, 2423
BspANI GGCC 3 cut(s) 156, 1934, 2349
BspCNI CTCAG 7 cut(s) 171, 550, 838, 1372, 1807, 2008, 2688
BspFNI CGCG 3 cut(s) 1020, 1124, 2670
BspHI TCATGA 1 cut(s) 2374
BspMAI CTGCAG 2 cut(s) 1711, 1923
BspPI GGATC 3 cut(s) 609, 619, 693
BspQI GCTCTTC 1 cut(s) 1800
BsrDI GCAATG 7 cut(s) 990, 1040, 1740, 1773, 2017, 2337, 2338
BsrFI RCCGGY 2 cut(s) 861, 1000
BsrI ACTGG 5 cut(s) 984, 1120, 1513, 1557, 1719
BssAI RCCGGY 2 cut(s) 861, 1000
BssECI CCNNGG 4 cut(s) 380, 946, 2109, 2703
BssT1I CCWWGG 2 cut(s) 380, 946
Bst2UI CCWGG 2 cut(s) 1212, 1826
Bst4CI ACNGT 4 cut(s) 455, 1505, 1621, 2240
Bst6I CTCTTC 8 cut(s) 630, 1023, 1062, 1686, 1800, 2154, 2193, 2220
BstDEI CTNAG 7 cut(s) 158, 537, 825, 1380, 1815, 2016, 2696
BstDSI CCRYGG 2 cut(s) 380, 2703
BstENI CCTNNNNNAGG 1 cut(s) 2614
BstF5I GGATG 9 cut(s) 93, 390, 405, 574, 1114, 1204, 1312, 1579, 1994
BstFNI CGCG 3 cut(s) 1020, 1124, 2670
BstHHI GCGC 1 cut(s) 1020
BstMAI GTCTC 8 cut(s) 624, 722, 1106, 1511, 1537, 1954, 2407, 2554
BstMCI CGRYCG 1 cut(s) 599
BstMWI GCNNNNNNNGC 4 cut(s) 327, 1238, 1715, 2576
BstNI CCWGG 2 cut(s) 1212, 1826
BstNSI RCATGY 1 cut(s) 1361
BstSCI CCNGG 3 cut(s) 1210, 1824, 2109
BstSFI CTRYAG 3 cut(s) 1152, 1707, 1919
BstUI CGCG 3 cut(s) 1020, 1124, 2670
BstV1I GCAGC 7 cut(s) 15, 449, 707, 1727, 1775, 1849, 1905
BstV2I GAAGAC 4 cut(s) 169, 1335, 1569, 2241
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 3 cut(s) 156, 1934, 2349
BtgI CCRYGG 2 cut(s) 380, 2703
BtgZI GCGATG 5 cut(s) 429, 1105, 1847, 1961, 2040
BtsCI GGATG 9 cut(s) 93, 390, 405, 574, 1114, 1204, 1312, 1579, 1994
BtsI GCAGTG 2 cut(s) 1716, 2599
BtsIMutI CAGTG 3 cut(s) 426, 1716, 2599
BtuMI TCGCGA 2 cut(s) 1124, 2670
CciI TCATGA 1 cut(s) 2374
CfoI GCGC 1 cut(s) 1020
Cfr10I RCCGGY 2 cut(s) 861, 1000
Cfr13I GGNCC 1 cut(s) 2700
Csp6I GTAC 3 cut(s) 925, 1789, 1819
CviAII CATG 8 cut(s) 25, 251, 381, 1358, 1373, 2297, 2375, 2549
CviQI GTAC 3 cut(s) 925, 1789, 1819
DdeI CTNAG 7 cut(s) 158, 537, 825, 1380, 1815, 2016, 2696
EaeI YGGCCR 1 cut(s) 2347
Eam1104I CTCTTC 8 cut(s) 630, 1023, 1062, 1686, 1800, 2154, 2193, 2220
EarI CTCTTC 8 cut(s) 630, 1023, 1062, 1686, 1800, 2154, 2193, 2220
EciI GGCGGA 1 cut(s) 1247
Eco130I CCWWGG 2 cut(s) 380, 946
Eco24I GRGCYC 1 cut(s) 207
Eco32I GATATC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 2700
Eco81I CCTNAGG 1 cut(s) 825
EcoNI CCTNNNNNAGG 1 cut(s) 2614
EcoRI GAATTC 2 cut(s) 1306, 1406
EcoRII CCWGG 2 cut(s) 1210, 1824
EcoRV GATATC 1 cut(s) 264
EcoT14I CCWWGG 2 cut(s) 380, 946
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 2 cut(s) 380, 946
Esp3I CGTCTC 3 cut(s) 624, 722, 1537
FaeI CATG 8 cut(s) 28, 254, 384, 1361, 1376, 2300, 2378, 2552
FalI AAGNNNNNCTT 6 cut(s) 1386, 1418, 1975, 2007, 2190, 2222
FaqI GGGAC 1 cut(s) 1261
FatI CATG 8 cut(s) 24, 250, 380, 1357, 1372, 2296, 2374, 2548
FauI CCCGC 2 cut(s) 901, 1857
FauNDI CATATG 1 cut(s) 1462
FbaI TGATCA 6 cut(s) 90, 571, 847, 1261, 2377, 2545
Fnu4HI GCNGC 8 cut(s) 6, 29, 463, 721, 1716, 1764, 1838, 1919
FokI GGATG 9 cut(s) 80, 397, 412, 581, 1121, 1211, 1299, 1586, 1981
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 8 cut(s) 6, 29, 463, 721, 1716, 1764, 1838, 1919
FspBI CTAG 4 cut(s) 62, 183, 1187, 2177
GlaI GCGC 1 cut(s) 1019
GluI GCNGC 8 cut(s) 6, 29, 463, 721, 1716, 1764, 1838, 1919
GsuI CTGGAG 1 cut(s) 2153
HaeIII GGCC 3 cut(s) 156, 1934, 2349
HapII CCGG 5 cut(s) 341, 862, 1001, 1998, 2111
HhaI GCGC 1 cut(s) 1020
Hin1II CATG 8 cut(s) 28, 254, 384, 1361, 1376, 2300, 2378, 2552
Hin6I GCGC 1 cut(s) 1018
HinP1I GCGC 1 cut(s) 1018
HindIII AAGCTT 2 cut(s) 1779, 2204
HpaII CCGG 5 cut(s) 341, 862, 1001, 1998, 2111
HphI GGTGA 6 cut(s) 281, 602, 775, 1175, 1616, 1870
HpyCH4III ACNGT 4 cut(s) 455, 1505, 1621, 2240
HpyF10VI GCNNNNNNNGC 4 cut(s) 327, 1238, 1715, 2576
HpyF3I CTNAG 7 cut(s) 158, 537, 825, 1380, 1815, 2016, 2696
Hsp92II CATG 8 cut(s) 28, 254, 384, 1361, 1376, 2300, 2378, 2552
HspAI GCGC 1 cut(s) 1018
Ksp22I TGATCA 6 cut(s) 90, 571, 847, 1261, 2377, 2545
LguI GCTCTTC 1 cut(s) 1800
LmnI GCTCC 2 cut(s) 1148, 1894
Lsp1109I GCAGC 7 cut(s) 15, 449, 707, 1727, 1775, 1849, 1905
LweI GCATC 5 cut(s) 1128, 1321, 1824, 2107, 2422
MaeI CTAG 4 cut(s) 62, 183, 1187, 2177
MaeIII GTNAC 7 cut(s) 424, 710, 763, 1555, 1589, 1615, 1746
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 1 cut(s) 207
MlsI TGGCCA 1 cut(s) 2349
MluNI TGGCCA 1 cut(s) 2349
MlyI GAGTC 6 cut(s) 79, 922, 1040, 1559, 1620, 2516
MmeI TCCRAC 4 cut(s) 579, 1126, 2175, 2490
Mox20I TGGCCA 1 cut(s) 2349
MroXI GAANNNNTTC 2 cut(s) 1206, 2664
MscI TGGCCA 1 cut(s) 2349
MseI TTAA 2 cut(s) 507, 1631
MslI CAYNNNNRTG 4 cut(s) 255, 1317, 1362, 2553
Msp20I TGGCCA 1 cut(s) 2349
MspI CCGG 5 cut(s) 341, 862, 1001, 1998, 2111
MspR9I CCNGG 3 cut(s) 1212, 1826, 2111
Mva1269I GAATGC 1 cut(s) 1533
MvaI CCWGG 2 cut(s) 1212, 1826
MvnI CGCG 3 cut(s) 1020, 1124, 2670
MwoI GCNNNNNNNGC 4 cut(s) 327, 1238, 1715, 2576
NciI CCSGG 1 cut(s) 2111
NcoI CCATGG 1 cut(s) 380
NdeI CATATG 1 cut(s) 1462
NlaIII CATG 8 cut(s) 28, 254, 384, 1361, 1376, 2300, 2378, 2552
NmuCI GTSAC 5 cut(s) 424, 710, 763, 1589, 1615
NruI TCGCGA 2 cut(s) 1124, 2670
NspI RCATGY 1 cut(s) 1361
PagI TCATGA 1 cut(s) 2374
PciI ACATGT 1 cut(s) 1357
PciSI GCTCTTC 1 cut(s) 1800
PcsI WCGNNNNNNNCGW 3 cut(s) 601, 1427, 2674
PctI GAATGC 1 cut(s) 1533
PdmI GAANNNNTTC 2 cut(s) 1206, 2664
PfeI GAWTC 8 cut(s) 245, 305, 403, 887, 1103, 1255, 2447, 2489
PkrI GCNGC 8 cut(s) 7, 30, 464, 722, 1717, 1765, 1839, 1920
PleI GAGTC 6 cut(s) 79, 921, 1039, 1559, 1619, 2515
PpsI GAGTC 6 cut(s) 79, 921, 1039, 1559, 1619, 2515
PscI ACATGT 1 cut(s) 1357
Psp6I CCWGG 2 cut(s) 1210, 1824
PspGI CCWGG 2 cut(s) 1210, 1824
PspPI GGNCC 1 cut(s) 2700
PstI CTGCAG 2 cut(s) 1711, 1923
PsuI RGATCY 1 cut(s) 232
RruI TCGCGA 2 cut(s) 1124, 2670
RsaI GTAC 3 cut(s) 926, 1790, 1820
RsaNI GTAC 3 cut(s) 925, 1789, 1819
RseI CAYNNNNRTG 4 cut(s) 255, 1317, 1362, 2553
SapI GCTCTTC 1 cut(s) 1800
SaqAI TTAA 2 cut(s) 507, 1631
SatI GCNGC 8 cut(s) 6, 29, 463, 721, 1716, 1764, 1838, 1919
Sau96I GGNCC 1 cut(s) 2700
SchI GAGTC 6 cut(s) 79, 922, 1040, 1559, 1620, 2516
ScrFI CCNGG 3 cut(s) 1212, 1826, 2111
SduI GDGCHC 1 cut(s) 207
SfaNI GCATC 5 cut(s) 1128, 1321, 1824, 2107, 2422
SfcI CTRYAG 3 cut(s) 1152, 1707, 1919
SinI GGWCC 1 cut(s) 2700
SmiMI CAYNNNNRTG 4 cut(s) 255, 1317, 1362, 2553
SmlI CTYRAG 4 cut(s) 1293, 2186, 2215, 2614
SmoI CTYRAG 4 cut(s) 1293, 2186, 2215, 2614
SsiI CCGC 9 cut(s) 465, 609, 894, 960, 1020, 1232, 1602, 1864, 2423
SspMI CTAG 4 cut(s) 62, 183, 1187, 2177
StyD4I CCNGG 3 cut(s) 1210, 1824, 2109
StyI CCWWGG 2 cut(s) 380, 946
TaaI ACNGT 4 cut(s) 455, 1505, 1621, 2240
TaqII GACCGA 1 cut(s) 585
TatI WGTACW 1 cut(s) 924
TfiI GAWTC 8 cut(s) 245, 305, 403, 887, 1103, 1255, 2447, 2489
Tru1I TTAA 2 cut(s) 507, 1631
Tru9I TTAA 2 cut(s) 507, 1631
TscAI CASTG 3 cut(s) 433, 1716, 2606
TseFI GTSAC 5 cut(s) 424, 710, 763, 1589, 1615
TseI GCWGC 8 cut(s) 5, 28, 462, 720, 1715, 1763, 1837, 1918
Tsp45I GTSAC 5 cut(s) 424, 710, 763, 1589, 1615
TspGWI ACGGA 6 cut(s) 596, 746, 1305, 1669, 2459, 2681
TspRI CASTG 3 cut(s) 433, 1716, 2606
VpaK11BI GGWCC 1 cut(s) 2700
XagI CCTNNNNNAGG 1 cut(s) 2614
XapI RAATTY 5 cut(s) 547, 816, 1306, 1406, 2652
XbaI TCTAGA 1 cut(s) 2176
XceI RCATGY 1 cut(s) 1361
XcmI CCANNNNNNNNNTGG 1 cut(s) 1726
XmnI GAANNNNTTC 2 cut(s) 1206, 2664
XspI CTAG 4 cut(s) 62, 183, 1187, 2177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.