MD16G1022600.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011757
Physical Location & Seq
Reverse (-)
1646937 .. 1650950
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1022600.v1.1.491

Sequence Viewer

Length: 2853 bp
ATGGCTGCCAACAAGTGCGCAACCATGCTGCACAGAAACACCAACAAGATCACTCTCGTTCTAATCTATGCACTCCTCGAATGGATTCTGATCATCCTCCTCCTTCTCAATTCCCTATTTTCTTATCTGATCGTAAAGTTTTCCGATTACTTTGGCCTCAAAAGACCCTGCCTTTGGTGTACTAGGCTCGATCATATCTTCGGGCCCAGAAACACCAACAATTCTTACAGAGATCTTGTCTGCGAATCTCATGCCAACGAAATATCCCAATTGGGTTACTGCTCAAATCACCAGAAACTAGCAGAATCGCAAGATATGTGCGAGGACTGCTCGTCGAAGCCGGATTGCGAAGAATGGTCAAAGAAGTTTGCTTTTTTTCCATGGATGAAGCAAATTGGGATGATTCGAGACGGCAACGAGAAAGTCACTGAAAATGGAGAGGACAGGTTGAACTGTTCTTGCTGCGGATTGAGCTTGAACAAGTTTTACCCTTCTTGTATTGTGATTAAACCCTCTTGGGAGGTTTTGGATTATGCCCAGAAACAGAATTTGAGTTTGGAAACAGGGGATGATCATCATACGGAAGAAGGTGATCGGTCGGATCGAAGCGGATCGGATTTCGAGATCGGAAACGAAGAGGGAGTTGAAGAACAGAAGGGAGAAAATAGGGGTTTTGAAGCAGAGGATCGTTCTGCTGCTTGTTCTGTATGTGACTATGGCTGCAAGGAGAAGGAGATTGCAGCCAATGGAGATGAAGAAGTGGAAAGAGTCATAGAAGAAGAACAAGAGCCCACGAAATATATTGTATCTCCGAAAATTCGACCTCAGGATTTGGAGTTCTACATTGATCAGGACGATAGTGGGTTGGTTCTGGTTGATTTGATTGATTCTCCAACCACCATTGGAAACCAGAGTAAACAGAAGTACAATAAGGTGGAAGAAGACCAAGGAAATAGTAGTGGTTGTGAAGATGTTATACTGGATTTCGACATTGGTTTCGAGAGGCAAGAAGCGAAACCGGTTGTGGAGAGTTGGCGCGGTCCAGAAAAGACTGAGGCATTGCTTTCGTTCGATGAGAGTAAGGAGGAAAGTAGGGTTTCGGGTATTGATTCGAAGGGTCCGGGCGAAACTGAGAGCGCAAAGGAGGAAAATGAACAAGTTGGAGCTATAGCAAATGGTGAAATTGTTTCAGATGTTCATCAAGAATTTGAGGACGATGTCCTTTCGGGCGACGACGAGATTGATGCAGAGGTCTCAATTGGGACAGAGATTCCTGATCAGGAACCGACTGACGAAGTTCAATCAGCTCAAGGACTTCTGCATTCGGATCTTTCGCACAACGATGAGACTGATGCAGAGGTATCAATAGGGACAGAGATTCCTGATCAGGAAGAGAATGACGAAGTTCAATCAGCTCAAGAACTTTTGTATTCGTATGCGTGTGAGCAGGAAGACCCTTCTACAAGTTCTGCCAATCTACATGATTGCGATCATCATGGTTCTGAGCAAGCAGAGGAAGAATTACTTGAATTCAAAACCTTGTCGGTTGAAACGAGTGAGAACGAAAAAGATAATCATTTCTCACTTGAGGTAGATAATGGTCTTGACACACCTACTTCAATAGACAGTCTCCATCAGTTTCGTAAGGGAATGCTACTATTCGAGAGACGAGAACTGGTAACAGATGACTCATTGGATGGAAGTGTCATGAGTGACACTGAAGGCGGTGATGGAGTCGTGACAGTTGAGAAATTGAAGACAGCGCTGAGAGCTGAACGGAAGGCTTTGAACGAATTATATGCAGAACTCGAGGAAGAGAGGAGCGCTTCTGCGGTGGCAGCCAGCCAGACAATGGCGATGATAAATAGGCTACAAGAAGAAAAAGCAGCAATGCAGATGGAAGCTTTGCAGTACCAGAGAATGATGGAAGAGCAGTCTGAGTACGACCAGGAAGCGATGCAACTCTTGAATGAGCTTATGGTGAAGCGGGAAAAGGAAAAGCAAGAGCTCGAAAAGGAGCTGGAAATATGTCGAAAGAAACTGCAGGATTATGAGGTCAAAGAGAAAATGATGATTTTGAGGAGAATGAAAGATGGAAGCACGAAGAGTAGGACTTCATCCGGTGCTTGTAGCAATGGTGAGGATAGCGATGGACTCTCTACTGATTTGAATAACGAATCGAAGGAAGAAGATTGCTTATATGGCCATGAGGAAAGTAGCAACCAAAACACCCCGGTGGATGCAGTGCTGTATCTAGAGGAATCTTTGGCTAGCTTTGAGGAAGAGAAGTTGTCCATTCTAGACCAGCTCAAGGAGTTGGAAGAGAAGCTTTTGACCTTGAACAACGAAGAGGAAGAAGAATATGACGGGGATATAAAACCAAGTGAGCATTTATTTTTGGAGAATGGAAATGGTTACCATGAAAGTTCGGATGTTAGTAGTGAAATAAATGGCAATGGCATTGCAAATGGCCATTCCAAAGAAATGAATGGAAATTTCCCGCATCAAGATAGAAAAATTGTCAAGGGCTTGAAGGCAAAGAGGCTTCTTCCGTTATTTGATGCCATCGAAACAAAAACAGAAGACGGGGAATTGAATGGAAACACAGAAGGGTATGATTCTTTTACGTCGCAAGACTCTGAGAGCAAGAAACTTGCTATTGAAGAGGAGGTTGATCATGTGTACGAGAGACTACAAGCACTTGAAGCAGATAGGGAGTTTCTAAAGCACTGCATTTCCTCCTTGAGGAAAGGAGATAAGGGATTACTTCTCCTCCAAGAAATTTTGGAACATCTTCGCGACCTGCGAAGTGTAGAACTCCGTCTGAGGACCATGGGGGACAGCGATCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

951

Amino Acids

107.33

Weight (kDa)

4.4

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 584 - 674 3e-32 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 19
Acc36I ACCTGC 1 cut(s) 2811
AccB7I CCANNNNNTGG 1 cut(s) 1852
AccII CGCG 2 cut(s) 1038, 2799
AciI CCGC 7 cut(s) 465, 609, 1038, 1725, 1832, 1987, 2501
AclWI GGATC 4 cut(s) 609, 619, 693, 1335
AcoI YGGCCR 2 cut(s) 2203, 2470
AcsI RAATTY 6 cut(s) 547, 816, 1205, 1529, 2494, 2781
AcuI CTGAAG 1 cut(s) 1740
AfaI GTAC 5 cut(s) 181, 926, 1913, 1943, 2684
AfeI AGCGCT 2 cut(s) 1764, 1825
AfiI CCNNNNNNNGG 4 cut(s) 174, 1852, 2311, 2745
AgeI ACCGGT 1 cut(s) 1018
AhdI GACNNNNNGTC 1 cut(s) 331
AjnI CCWGG 1 cut(s) 1947
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
AleI CACNNNNGTG 1 cut(s) 2234
AloI GAACNNNNNNTCC 2 cut(s) 821, 853
Alw21I GWGCWC 1 cut(s) 2010
Alw26I GTCTC 6 cut(s) 402, 1258, 1340, 1634, 1660, 2683
AlwI GGATC 4 cut(s) 609, 619, 693, 1335
Ama87I CYCGRG 1 cut(s) 1808
Aor51HI AGCGCT 2 cut(s) 1764, 1825
AoxI GGCC 4 cut(s) 154, 203, 2203, 2470
ApaI GGGCCC 1 cut(s) 207
ApeKI GCWGC 8 cut(s) 5, 28, 462, 695, 720, 740, 1838, 1886
ApoI RAATTY 6 cut(s) 547, 816, 1205, 1529, 2494, 2781
ArsI GACNNNNNNTTYG 4 cut(s) 156, 188, 980, 1012
AsiGI ACCGGT 1 cut(s) 1018
Asp700I GAANNNNTTC 2 cut(s) 84, 2793
AspLEI GCGC 5 cut(s) 20, 1038, 1139, 1765, 1826
AspS9I GGNCC 5 cut(s) 203, 204, 1040, 1118, 2829
AsuC2I CCSGG 2 cut(s) 1122, 2234
AsuHPI GGTGA 6 cut(s) 281, 602, 1190, 1739, 1993, 2150
AsuII TTCGAA 1 cut(s) 1112
AsuNHI GCTAGC 1 cut(s) 2270
AvaI CYCGRG 1 cut(s) 1808
AvaII GGWCC 3 cut(s) 1040, 1118, 2829
AxyI CCTNAGG 1 cut(s) 825
BaeGI GKGCMC 1 cut(s) 207
BalI TGGCCA 2 cut(s) 2205, 2472
BanII GRGCYC 3 cut(s) 207, 792, 2010
BarI GAAGNNNNNNTAC 2 cut(s) 960, 992
BbsI GAAGAC 4 cut(s) 948, 1458, 1763, 2589
Bbv12I GWGCWC 1 cut(s) 2010
BbvI GCAGC 7 cut(s) 15, 449, 682, 707, 752, 1850, 1898
BccI CCATC 8 cut(s) 1641, 1691, 1724, 1891, 1918, 2087, 2144, 2573
BceAI ACGGC 1 cut(s) 427
BcgI CGANNNNNNTGC 8 cut(s) 233, 267, 1047, 1081, 1226, 1260, 1781, 1815
BciT130I CCWGG 1 cut(s) 1949
BclI TGATCA 6 cut(s) 90, 571, 847, 1276, 1384, 2674
BcnI CCSGG 2 cut(s) 1122, 2234
BcoDI GTCTC 6 cut(s) 402, 1258, 1340, 1634, 1660, 2683
BfaI CTAG 5 cut(s) 183, 299, 2255, 2271, 2300
BfmI CTRYAG 2 cut(s) 1167, 2042
BfoI RGCGCY 2 cut(s) 1766, 1827
BfuAI ACCTGC 1 cut(s) 2811
BglII AGATCT 1 cut(s) 232
BisI GCNGC 8 cut(s) 6, 29, 463, 696, 721, 741, 1839, 1887
BlsI GCNGC 8 cut(s) 7, 30, 464, 697, 722, 742, 1840, 1888
Bme1390I CCNGG 3 cut(s) 1122, 1949, 2234
Bme18I GGWCC 3 cut(s) 1040, 1118, 2829
BmeRI GACNNNNNGTC 1 cut(s) 331
BmeT110I CYCGRG 1 cut(s) 1808
BmgT120I GGNCC 5 cut(s) 203, 204, 1040, 1118, 2829
BmiI GGNNCC 3 cut(s) 205, 1119, 1284
BmrFI CCNGG 3 cut(s) 1122, 1949, 2234
BmsI GCATC 6 cut(s) 1234, 1342, 1947, 2230, 2512, 2551
BmtI GCTAGC 1 cut(s) 2274
BpiI GAAGAC 4 cut(s) 948, 1458, 1763, 2589
BplI GAGNNNNNCTC 2 cut(s) 314, 346
Bpu14I TTCGAA 1 cut(s) 1112
BpuEI CTTGAG 5 cut(s) 1293, 1401, 1607, 2294, 2764
BpuMI CCSGG 2 cut(s) 1122, 2234
BsaBI GATNNNNATC 4 cut(s) 89, 573, 1197, 1488
BsaI GGTCTC 1 cut(s) 1258
BsaJI CCNNGG 4 cut(s) 380, 946, 2232, 2832
BsaWI WCCGGW 2 cut(s) 1018, 2120
BsaXI ACNNNNNCTCC 2 cut(s) 2757, 2787
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 1852, 2311, 2745
Bse118I RCCGGY 1 cut(s) 1018
Bse1I ACTGG 2 cut(s) 984, 1680
Bse21I CCTNAGG 1 cut(s) 825
Bse3DI GCAATG 5 cut(s) 1058, 1896, 2140, 2460, 2461
Bse8I GATNNNNATC 4 cut(s) 89, 573, 1197, 1488
BseBI CCWGG 1 cut(s) 1949
BseDI CCNNGG 4 cut(s) 380, 946, 2232, 2832
BseGI GGATG 8 cut(s) 93, 390, 405, 574, 1702, 2117, 2245, 2437
BseJI GATNNNNATC 4 cut(s) 89, 573, 1197, 1488
BseLI CCNNNNNNNGG 4 cut(s) 174, 1852, 2311, 2745
BseMI GCAATG 5 cut(s) 1058, 1896, 2140, 2460, 2461
BseMII CTCAG 8 cut(s) 839, 1044, 1122, 1494, 1757, 1929, 2631, 2816
BseNI ACTGG 2 cut(s) 984, 1680
BseRI GAGGAG 6 cut(s) 65, 89, 1834, 2095, 2681, 2762
BseSI GKGCMC 1 cut(s) 207
BseXI GCAGC 7 cut(s) 15, 449, 682, 707, 752, 1850, 1898
BsgI GTGCAG 1 cut(s) 14
Bsh1236I CGCG 2 cut(s) 1038, 2799
Bsh1285I CGRYCG 1 cut(s) 599
BshFI GGCC 4 cut(s) 156, 205, 2205, 2472
BshTI ACCGGT 1 cut(s) 1018
BsiEI CGRYCG 1 cut(s) 599
BsiHKAI GWGCWC 1 cut(s) 2010
BsiHKCI CYCGRG 1 cut(s) 1808
BsiSI CCGG 5 cut(s) 341, 1019, 1121, 2121, 2234
BslFI GGGAC 2 cut(s) 1276, 1384
BslI CCNNNNNNNGG 4 cut(s) 174, 1852, 2311, 2745
BsmAI GTCTC 6 cut(s) 402, 1258, 1340, 1634, 1660, 2683
BsmBI CGTCTC 2 cut(s) 402, 1660
BsmFI GGGAC 2 cut(s) 1276, 1384
BsmI GAATGC 2 cut(s) 1321, 1656
BsnI GGCC 4 cut(s) 156, 205, 2205, 2472
Bso31I GGTCTC 1 cut(s) 1258
BsoBI CYCGRG 1 cut(s) 1808
Bsp119I TTCGAA 1 cut(s) 1112
Bsp120I GGGCCC 1 cut(s) 203
Bsp1286I GDGCHC 3 cut(s) 207, 792, 2010
Bsp19I CCATGG 2 cut(s) 380, 2832
Bsp68I TCGCGA 1 cut(s) 2799
BspACI CCGC 7 cut(s) 465, 609, 1038, 1725, 1832, 1987, 2501
BspANI GGCC 4 cut(s) 156, 205, 2205, 2472
BspCNI CTCAG 8 cut(s) 838, 1045, 1123, 1495, 1758, 1930, 2632, 2817
BspFNI CGCG 2 cut(s) 1038, 2799
BspHI TCATGA 1 cut(s) 1707
BspLI GGNNCC 3 cut(s) 205, 1119, 1284
BspMAI CTGCAG 1 cut(s) 2046
BspMI ACCTGC 1 cut(s) 2811
BspOI GCTAGC 1 cut(s) 2274
BspPI GGATC 4 cut(s) 609, 619, 693, 1335
BspQI GCTCTTC 1 cut(s) 1923
BspT104I TTCGAA 1 cut(s) 1112
BspTNI GGTCTC 1 cut(s) 1258
BsrDI GCAATG 5 cut(s) 1058, 1896, 2140, 2460, 2461
BsrFI RCCGGY 1 cut(s) 1018
BsrI ACTGG 2 cut(s) 984, 1680
BssAI RCCGGY 1 cut(s) 1018
BssECI CCNNGG 4 cut(s) 380, 946, 2232, 2832
BssT1I CCWWGG 3 cut(s) 380, 946, 2832
Bst2UI CCWGG 1 cut(s) 1949
Bst4CI ACNGT 3 cut(s) 455, 1628, 1744
Bst6I CTCTTC 9 cut(s) 630, 1386, 1809, 1923, 2099, 2277, 2316, 2343, 2658
BstBI TTCGAA 1 cut(s) 1112
BstC8I GCNNGC 3 cut(s) 1509, 1843, 2272
BstDEI CTNAG 8 cut(s) 825, 1053, 1131, 1503, 1766, 1938, 2640, 2825
BstDSI CCRYGG 2 cut(s) 380, 2832
BstEII GGTNACC 1 cut(s) 2414
BstENI CCTNNNNNAGG 1 cut(s) 2743
BstF5I GGATG 8 cut(s) 93, 390, 405, 574, 1702, 2117, 2245, 2437
BstFNI CGCG 2 cut(s) 1038, 2799
BstH2I RGCGCY 2 cut(s) 1766, 1827
BstHHI GCGC 5 cut(s) 20, 1038, 1139, 1765, 1826
BstMAI GTCTC 6 cut(s) 402, 1258, 1340, 1634, 1660, 2683
BstMCI CGRYCG 1 cut(s) 599
BstMWI GCNNNNNNNGC 6 cut(s) 327, 471, 1769, 1838, 2202, 2705
BstNI CCWGG 1 cut(s) 1949
BstPI GGTNACC 1 cut(s) 2414
BstSCI CCNGG 3 cut(s) 1120, 1947, 2232
BstSFI CTRYAG 2 cut(s) 1167, 2042
BstSLI GKGCMC 1 cut(s) 207
BstUI CGCG 2 cut(s) 1038, 2799
BstV1I GCAGC 7 cut(s) 15, 449, 682, 707, 752, 1850, 1898
BstV2I GAAGAC 4 cut(s) 948, 1458, 1763, 2589
BstX2I RGATCY 2 cut(s) 232, 1327
BstYI RGATCY 2 cut(s) 232, 1327
Bsu36I CCTNAGG 1 cut(s) 825
BsuRI GGCC 4 cut(s) 156, 205, 2205, 2472
BtgI CCRYGG 2 cut(s) 380, 2832
BtgZI GCGATG 3 cut(s) 1871, 1970, 2163
BtsCI GGATG 8 cut(s) 93, 390, 405, 574, 1702, 2117, 2245, 2437
BtsI GCAGTG 2 cut(s) 2250, 2728
BtsIMutI CAGTG 4 cut(s) 426, 1716, 2250, 2728
BtuMI TCGCGA 1 cut(s) 2799
BveI ACCTGC 1 cut(s) 2811
Cac8I GCNNGC 3 cut(s) 1509, 1843, 2272
CciI TCATGA 1 cut(s) 1707
CfoI GCGC 5 cut(s) 20, 1038, 1139, 1765, 1826
Cfr10I RCCGGY 1 cut(s) 1018
Cfr13I GGNCC 5 cut(s) 203, 204, 1040, 1118, 2829
Csp6I GTAC 5 cut(s) 180, 925, 1912, 1942, 2683
CspAI ACCGGT 1 cut(s) 1018
CviQI GTAC 5 cut(s) 180, 925, 1912, 1942, 2683
DdeI CTNAG 8 cut(s) 825, 1053, 1131, 1503, 1766, 1938, 2640, 2825
DriI GACNNNNNGTC 1 cut(s) 331
EaeI YGGCCR 2 cut(s) 2203, 2470
Eam1104I CTCTTC 9 cut(s) 630, 1386, 1809, 1923, 2099, 2277, 2316, 2343, 2658
Eam1105I GACNNNNNGTC 1 cut(s) 331
EarI CTCTTC 9 cut(s) 630, 1386, 1809, 1923, 2099, 2277, 2316, 2343, 2658
Ecl136II GAGCTC 1 cut(s) 2008
Eco130I CCWWGG 3 cut(s) 380, 946, 2832
Eco24I GRGCYC 3 cut(s) 207, 792, 2010
Eco31I GGTCTC 1 cut(s) 1258
Eco47I GGWCC 3 cut(s) 1040, 1118, 2829
Eco47III AGCGCT 2 cut(s) 1764, 1825
Eco53kI GAGCTC 1 cut(s) 2008
Eco57I CTGAAG 1 cut(s) 1740
Eco81I CCTNAGG 1 cut(s) 825
Eco88I CYCGRG 1 cut(s) 1808
Eco91I GGTNACC 1 cut(s) 2414
EcoICRI GAGCTC 1 cut(s) 2008
EcoNI CCTNNNNNAGG 1 cut(s) 2743
EcoO65I GGTNACC 1 cut(s) 2414
EcoRI GAATTC 1 cut(s) 1529
EcoRII CCWGG 1 cut(s) 1947
EcoT14I CCWWGG 3 cut(s) 380, 946, 2832
EcoT38I GRGCYC 3 cut(s) 207, 792, 2010
ErhI CCWWGG 3 cut(s) 380, 946, 2832
Esp3I CGTCTC 2 cut(s) 402, 1660
FalI AAGNNNNNCTT 4 cut(s) 1509, 1541, 2313, 2345
FaqI GGGAC 2 cut(s) 1276, 1384
FauI CCCGC 2 cut(s) 1980, 2508
FbaI TGATCA 6 cut(s) 90, 571, 847, 1276, 1384, 2674
Fnu4HI GCNGC 8 cut(s) 6, 29, 463, 696, 721, 741, 1839, 1887
FokI GGATG 8 cut(s) 80, 397, 412, 581, 1709, 2104, 2252, 2444
FriOI GRGCYC 3 cut(s) 207, 792, 2010
Fsp4HI GCNGC 8 cut(s) 6, 29, 463, 696, 721, 741, 1839, 1887
FspBI CTAG 5 cut(s) 183, 299, 2255, 2271, 2300
FspI TGCGCA 1 cut(s) 19
GlaI GCGC 5 cut(s) 19, 1037, 1138, 1764, 1825
GluI GCNGC 8 cut(s) 6, 29, 463, 696, 721, 741, 1839, 1887
HaeII RGCGCY 2 cut(s) 1766, 1827
HaeIII GGCC 4 cut(s) 156, 205, 2205, 2472
HapII CCGG 5 cut(s) 341, 1019, 1121, 2121, 2234
HhaI GCGC 5 cut(s) 20, 1038, 1139, 1765, 1826
Hin6I GCGC 5 cut(s) 18, 1036, 1137, 1763, 1824
HinP1I GCGC 5 cut(s) 18, 1036, 1137, 1763, 1824
HindIII AAGCTT 2 cut(s) 1902, 2327
HpaII CCGG 5 cut(s) 341, 1019, 1121, 2121, 2234
HphI GGTGA 6 cut(s) 281, 602, 1190, 1739, 1993, 2150
Hpy166II GTNNAC 3 cut(s) 180, 917, 2683
Hpy8I GTNNAC 3 cut(s) 180, 917, 2683
Hpy99I CGWCG 4 cut(s) 337, 1235, 1238, 2632
HpyCH4III ACNGT 3 cut(s) 455, 1628, 1744
HpyCH4IV ACGT 1 cut(s) 2627
HpyF10VI GCNNNNNNNGC 6 cut(s) 327, 471, 1769, 1838, 2202, 2705
HpyF3I CTNAG 8 cut(s) 825, 1053, 1131, 1503, 1766, 1938, 2640, 2825
HpySE526I ACGT 1 cut(s) 2627
HspAI GCGC 5 cut(s) 18, 1036, 1137, 1763, 1824
Ksp22I TGATCA 6 cut(s) 90, 571, 847, 1276, 1384, 2674
LguI GCTCTTC 1 cut(s) 1923
LmnI GCTCC 3 cut(s) 1163, 1821, 2017
Lsp1109I GCAGC 7 cut(s) 15, 449, 682, 707, 752, 1850, 1898
LweI GCATC 6 cut(s) 1234, 1342, 1947, 2230, 2512, 2551
MaeI CTAG 5 cut(s) 183, 299, 2255, 2271, 2300
MaeII ACGT 1 cut(s) 2627
MaeIII GTNAC 7 cut(s) 275, 424, 710, 1678, 1712, 1738, 2414
MfeI CAATTG 2 cut(s) 269, 1257
MflI RGATCY 2 cut(s) 232, 1327
MhlI GDGCHC 3 cut(s) 207, 792, 2010
MlsI TGGCCA 2 cut(s) 2205, 2472
MluNI TGGCCA 2 cut(s) 2205, 2472
MlyI GAGTC 5 cut(s) 777, 1682, 1743, 2148, 2630
MmeI TCCRAC 4 cut(s) 579, 917, 1141, 2298
Mox20I TGGCCA 2 cut(s) 2205, 2472
MroXI GAANNNNTTC 2 cut(s) 84, 2793
MscI TGGCCA 2 cut(s) 2205, 2472
MseI TTAA 1 cut(s) 507
MslI CAYNNNNRTG 2 cut(s) 1341, 2234
Msp20I TGGCCA 2 cut(s) 2205, 2472
MspI CCGG 5 cut(s) 341, 1019, 1121, 2121, 2234
MspR9I CCNGG 3 cut(s) 1122, 1949, 2234
MunI CAATTG 2 cut(s) 269, 1257
Mva1269I GAATGC 2 cut(s) 1321, 1656
MvaI CCWGG 1 cut(s) 1949
MvnI CGCG 2 cut(s) 1038, 2799
MwoI GCNNNNNNNGC 6 cut(s) 327, 471, 1769, 1838, 2202, 2705
NciI CCSGG 2 cut(s) 1122, 2234
NcoI CCATGG 2 cut(s) 380, 2832
NheI GCTAGC 1 cut(s) 2270
NlaIV GGNNCC 3 cut(s) 205, 1119, 1284
NmuCI GTSAC 4 cut(s) 424, 710, 1712, 1738
NruI TCGCGA 1 cut(s) 2799
NsbI TGCGCA 1 cut(s) 19
NspV TTCGAA 1 cut(s) 1112
OliI CACNNNNGTG 1 cut(s) 2234
PaeR7I CTCGAG 1 cut(s) 1808
PagI TCATGA 1 cut(s) 1707
PciSI GCTCTTC 1 cut(s) 1923
PcsI WCGNNNNNNNCGW 3 cut(s) 601, 1550, 2803
PctI GAATGC 2 cut(s) 1321, 1656
PdmI GAANNNNTTC 2 cut(s) 84, 2793
PflFI GACNNNGTC 1 cut(s) 1217
PflMI CCANNNNNTGG 1 cut(s) 1852
PinAI ACCGGT 1 cut(s) 1018
PkrI GCNGC 8 cut(s) 7, 30, 464, 697, 722, 742, 1840, 1888
PleI GAGTC 5 cut(s) 776, 1682, 1742, 2148, 2630
PpsI GAGTC 5 cut(s) 776, 1682, 1742, 2148, 2630
Psp124BI GAGCTC 1 cut(s) 2010
Psp6I CCWGG 1 cut(s) 1947
PspEI GGTNACC 1 cut(s) 2414
PspGI CCWGG 1 cut(s) 1947
PspN4I GGNNCC 3 cut(s) 205, 1119, 1284
PspOMI GGGCCC 1 cut(s) 203
PspPI GGNCC 5 cut(s) 203, 204, 1040, 1118, 2829
PspXI VCTCGAGB 1 cut(s) 1808
PsrI GAACNNNNNNTAC 2 cut(s) 470, 502
PstI CTGCAG 1 cut(s) 2046
PsuI RGATCY 2 cut(s) 232, 1327
PsyI GACNNNGTC 1 cut(s) 1217
RruI TCGCGA 1 cut(s) 2799
RsaI GTAC 5 cut(s) 181, 926, 1913, 1943, 2684
RsaNI GTAC 5 cut(s) 180, 925, 1912, 1942, 2683
RseI CAYNNNNRTG 2 cut(s) 1341, 2234
SacI GAGCTC 1 cut(s) 2010
SapI GCTCTTC 1 cut(s) 1923
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 8 cut(s) 6, 29, 463, 696, 721, 741, 1839, 1887
Sau96I GGNCC 5 cut(s) 203, 204, 1040, 1118, 2829
SchI GAGTC 5 cut(s) 777, 1682, 1743, 2148, 2630
ScrFI CCNGG 3 cut(s) 1122, 1949, 2234
SduI GDGCHC 3 cut(s) 207, 792, 2010
SfaNI GCATC 6 cut(s) 1234, 1342, 1947, 2230, 2512, 2551
SfcI CTRYAG 2 cut(s) 1167, 2042
Sfr274I CTCGAG 1 cut(s) 1808
SfuI TTCGAA 1 cut(s) 1112
SinI GGWCC 3 cut(s) 1040, 1118, 2829
SlaI CTCGAG 1 cut(s) 1808
SmiMI CAYNNNNRTG 2 cut(s) 1341, 2234
SmlI CTYRAG 6 cut(s) 1308, 1416, 1586, 1808, 2309, 2743
SmoI CTYRAG 6 cut(s) 1308, 1416, 1586, 1808, 2309, 2743
SsiI CCGC 7 cut(s) 465, 609, 1038, 1725, 1832, 1987, 2501
SspMI CTAG 5 cut(s) 183, 299, 2255, 2271, 2300
SstI GAGCTC 1 cut(s) 2010
StyD4I CCNGG 3 cut(s) 1120, 1947, 2232
StyI CCWWGG 3 cut(s) 380, 946, 2832
TaaI ACNGT 3 cut(s) 455, 1628, 1744
TaiI ACGT 1 cut(s) 2630
TaqII GACCGA 1 cut(s) 585
TatI WGTACW 2 cut(s) 179, 924
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 4 cut(s) 433, 1723, 2250, 2735
TseFI GTSAC 4 cut(s) 424, 710, 1712, 1738
TseI GCWGC 8 cut(s) 5, 28, 462, 695, 720, 740, 1838, 1886
Tsp45I GTSAC 4 cut(s) 424, 710, 1712, 1738
TspDTI ATGAA 8 cut(s) 401, 768, 1167, 1187, 2102, 2106, 2436, 2501
TspGWI ACGGA 4 cut(s) 596, 1792, 2541, 2810
TspRI CASTG 4 cut(s) 433, 1723, 2250, 2735
Tth111I GACNNNGTC 1 cut(s) 1217
Van91I CCANNNNNTGG 1 cut(s) 1852
VpaK11BI GGWCC 3 cut(s) 1040, 1118, 2829
XagI CCTNNNNNAGG 1 cut(s) 2743
XapI RAATTY 6 cut(s) 547, 816, 1205, 1529, 2494, 2781
XbaI TCTAGA 2 cut(s) 2254, 2299
XcmI CCANNNNNNNNNTGG 1 cut(s) 1849
XhoI CTCGAG 1 cut(s) 1808
XmnI GAANNNNTTC 2 cut(s) 84, 2793
XspI CTAG 5 cut(s) 183, 299, 2255, 2271, 2300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.