Rmu_sc0001118.1_g000021

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001118.1
OG0011757
Physical Location & Seq
Forward (+)
135257 .. 136531
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001118.1_g000021.1.cds

Sequence Viewer

Length: 1275 bp
atggctgccaacaagtttgccaccatgttacacagaaacaccaacaaactcactctgattctcatctacgcaatcctcgaatgggttctgatcctcctcctccttctcaactccctcttctcttatctgatcgtcaagttcgccgattactttggcctcaaacgaccctgcctctggtgttctaggctcgatcacatcttcgagcccggaaacaagagccacagttcttacagagatcttgtgtgtgaagaacacgccaatgaaatagccaaacttgggttttgctcgaatcaccagaaactggctgagtctcaagatatgtgtgaggattgctcatcgcaggaatattctcaagaatggtctaacaagtatgctttctttccgtggatgaagcaaattgggatgattcaaggcggcgatgatgataaggagattaacgataaggaatgttcttgctgcggtgtgagcttgagcaacaagctgtaccctccttgtattctgctcaagccttcttggggggttttggattacgctcagaaacccattttgagttcagatgtaggggttgatgctcagactgaggaaggtgatcagtcggatcaaagtggatcggattatgtggcagatcaaaatggacatgacgagggtgttgttgttgttgttgttcaaggaaataggggagagaattcggtttcggtttcggatgttgatggatgttgtggacaaagagactatgatgcagaggagaagaataattcagcatgttgttctgtttgtgactatgtctgcaaggaaacggaaactgaggccaatcaaattgaagacgaggctaatatgaatgtctccatggacgatggtgatgatggtcgagctcaaattcaggccagttgtgatgagaataagtctcgtggaagtctgcatcagcatctggagttctacattgatcaagatgattgtcggttggttccggttgaattggttgattctcctgccaccatcgagaggcaaagtcaccagaattataaggtggaggaagaccgagcacatagtgatactcaagatgttattctggatttcgacatgcatttcgagacacaagccaaaccggttgttgagttgtggcacagttcagatgacattgtggaattactttcatcccatgagagtaaagaggagagcaaagtttcagttcttgactcaaaggatttggatttcgatatgcatttcaagacacaagccaaaccggttaattgtggaattactttcatcccatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

424

Amino Acids

47.76

Weight (kDa)

4.47

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
AccB7I CCANNNNNTGG 1 cut(s) 301
AciI CCGC 2 cut(s) 414, 459
AclWI GGATC 3 cut(s) 85, 606, 616
AcsI RAATTY 2 cut(s) 685, 876
AdeI CACNNNGTG 1 cut(s) 1049
AfaI GTAC 1 cut(s) 485
AfiI CCNNNNNNNGG 6 cut(s) 82, 174, 276, 301, 515, 1002
AgeI ACCGGT 2 cut(s) 1105, 1243
AgsI TTSAA 5 cut(s) 410, 668, 821, 974, 1228
AluBI AGCT 3 cut(s) 468, 481, 872
AluI AGCT 3 cut(s) 468, 481, 872
Alw21I GWGCWC 2 cut(s) 874, 1045
Alw26I GTCTC 5 cut(s) 315, 723, 847, 909, 1085
AlwI GGATC 3 cut(s) 85, 606, 616
AlwNI CAGNNNCTG 2 cut(s) 301, 928
AoxI GGCC 3 cut(s) 154, 807, 882
ApeKI GCWGC 2 cut(s) 5, 456
ApoI RAATTY 2 cut(s) 685, 876
ArsI GACNNNNNNTTYG 2 cut(s) 1070, 1102
AsiGI ACCGGT 2 cut(s) 1105, 1243
Asp700I GAANNNNTTC 1 cut(s) 84
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 4 cut(s) 284, 599, 869, 1004
BanII GRGCYC 2 cut(s) 207, 874
BauI CACGAG 1 cut(s) 906
BbsI GAAGAC 2 cut(s) 828, 1041
Bbv12I GWGCWC 2 cut(s) 874, 1045
BbvI GCAGC 1 cut(s) 443
BccI CCATC 4 cut(s) 704, 848, 857, 1004
BclI TGATCA 2 cut(s) 589, 943
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 5 cut(s) 315, 723, 847, 909, 1085
BfaI CTAG 1 cut(s) 183
BglII AGATCT 1 cut(s) 235
BisI GCNGC 3 cut(s) 6, 415, 457
BlsI GCNGC 3 cut(s) 7, 416, 458
Bme1390I CCNGG 1 cut(s) 207
BmiI GGNNCC 1 cut(s) 966
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 4 cut(s) 559, 727, 928, 934
BpiI GAAGAC 2 cut(s) 828, 1041
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 950
BpuEI CTTGAG 5 cut(s) 297, 336, 488, 490, 1041
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 383, 846
BsaWI WCCGGW 3 cut(s) 967, 1105, 1243
Bsc4I CCNNNNNNNGG 6 cut(s) 82, 174, 276, 301, 515, 1002
Bse118I RCCGGY 2 cut(s) 1105, 1243
Bse1I ACTGG 2 cut(s) 306, 885
Bse8I GATNNNNATC 1 cut(s) 62
BseDI CCNNGG 2 cut(s) 383, 846
BseGI GGATG 6 cut(s) 393, 408, 709, 719, 1154, 1266
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 6 cut(s) 82, 174, 276, 301, 515, 1002
BseMII CTCAG 5 cut(s) 297, 548, 570, 587, 795
BseNI ACTGG 2 cut(s) 306, 885
BseRI GAGGAG 4 cut(s) 86, 89, 758, 1187
BseXI GCAGC 1 cut(s) 443
BshFI GGCC 3 cut(s) 156, 809, 884
BshTI ACCGGT 2 cut(s) 1105, 1243
BsiHKAI GWGCWC 2 cut(s) 874, 1045
BsiSI CCGG 4 cut(s) 207, 968, 1106, 1244
BslI CCNNNNNNNGG 6 cut(s) 82, 174, 276, 301, 515, 1002
BsmAI GTCTC 5 cut(s) 315, 723, 847, 909, 1085
BsnI GGCC 3 cut(s) 156, 809, 884
Bsp1286I GDGCHC 3 cut(s) 207, 874, 1045
Bsp143I GATC 9 cut(s) 90, 129, 190, 235, 589, 598, 608, 625, 943
Bsp19I CCATGG 1 cut(s) 846
BspACI CCGC 2 cut(s) 414, 459
BspANI GGCC 3 cut(s) 156, 809, 884
BspCNI CTCAG 5 cut(s) 298, 547, 571, 586, 796
BspLI GGNNCC 1 cut(s) 966
BspPI GGATC 3 cut(s) 85, 606, 616
BsrFI RCCGGY 2 cut(s) 1105, 1243
BsrI ACTGG 2 cut(s) 306, 885
BssAI RCCGGY 2 cut(s) 1105, 1243
BssECI CCNNGG 2 cut(s) 383, 846
BssMI GATC 9 cut(s) 90, 129, 190, 235, 589, 598, 608, 625, 943
BssSI CACGAG 1 cut(s) 906
BssT1I CCWWGG 1 cut(s) 846
Bst2BI CACGAG 1 cut(s) 906
Bst4CI ACNGT 2 cut(s) 224, 1127
Bst6I CTCTTC 1 cut(s) 122
BstDEI CTNAG 5 cut(s) 306, 534, 573, 579, 804
BstDSI CCRYGG 2 cut(s) 383, 846
BstF5I GGATG 6 cut(s) 393, 408, 709, 719, 1154, 1266
BstKTI GATC 9 cut(s) 93, 132, 193, 238, 592, 601, 611, 628, 946
BstMAI GTCTC 5 cut(s) 315, 723, 847, 909, 1085
BstMBI GATC 9 cut(s) 90, 129, 190, 235, 589, 598, 608, 625, 943
BstMWI GCNNNNNNNGC 1 cut(s) 465
BstNSI RCATGY 2 cut(s) 765, 1084
BstSCI CCNGG 1 cut(s) 205
BstV1I GCAGC 1 cut(s) 443
BstV2I GAAGAC 2 cut(s) 828, 1041
BstX2I RGATCY 1 cut(s) 235
BstYI RGATCY 1 cut(s) 235
BsuRI GGCC 3 cut(s) 156, 809, 884
BtgI CCRYGG 2 cut(s) 383, 846
BtgZI GCGATG 2 cut(s) 321, 432
BtsCI GGATG 6 cut(s) 393, 408, 709, 719, 1154, 1266
CaiI CAGNNNCTG 2 cut(s) 301, 928
Cfr10I RCCGGY 2 cut(s) 1105, 1243
Csp6I GTAC 1 cut(s) 484
CspAI ACCGGT 2 cut(s) 1105, 1243
CviAII CATG 7 cut(s) 25, 638, 762, 847, 1081, 1160, 1272
CviQI GTAC 1 cut(s) 484
DdeI CTNAG 5 cut(s) 306, 534, 573, 579, 804
DpnI GATC 9 cut(s) 92, 131, 192, 237, 591, 600, 610, 627, 945
DpnII GATC 9 cut(s) 90, 129, 190, 235, 589, 598, 608, 625, 943
DraIII CACNNNGTG 1 cut(s) 1049
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Ecl136II GAGCTC 1 cut(s) 872
Eco130I CCWWGG 1 cut(s) 846
Eco24I GRGCYC 2 cut(s) 207, 874
Eco53kI GAGCTC 1 cut(s) 872
EcoICRI GAGCTC 1 cut(s) 872
EcoRI GAATTC 1 cut(s) 685
EcoT14I CCWWGG 1 cut(s) 846
EcoT22I ATGCAT 2 cut(s) 1086, 1224
EcoT38I GRGCYC 2 cut(s) 207, 874
ErhI CCWWGG 1 cut(s) 846
FaeI CATG 7 cut(s) 28, 641, 765, 850, 1084, 1163, 1275
FatI CATG 7 cut(s) 24, 637, 761, 846, 1080, 1159, 1271
FbaI TGATCA 2 cut(s) 589, 943
Fnu4HI GCNGC 3 cut(s) 6, 415, 457
FokI GGATG 6 cut(s) 400, 415, 716, 726, 1141, 1253
FriOI GRGCYC 2 cut(s) 207, 874
Fsp4HI GCNGC 3 cut(s) 6, 415, 457
FspBI CTAG 1 cut(s) 183
GluI GCNGC 3 cut(s) 6, 415, 457
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 3 cut(s) 156, 809, 884
HapII CCGG 4 cut(s) 207, 968, 1106, 1244
Hin1II CATG 7 cut(s) 28, 641, 765, 850, 1084, 1163, 1275
HinfI GANTC 6 cut(s) 58, 289, 308, 406, 983, 1196
HpaII CCGG 4 cut(s) 207, 968, 1106, 1244
HphI GGTGA 4 cut(s) 284, 599, 869, 1004
Hpy166II GTNNAC 1 cut(s) 722
Hpy8I GTNNAC 1 cut(s) 722
HpyAV CCTTC 3 cut(s) 113, 519, 578
HpyCH4III ACNGT 2 cut(s) 224, 1127
HpyCH4V TGCA 5 cut(s) 740, 789, 919, 1084, 1222
HpyF10VI GCNNNNNNNGC 1 cut(s) 465
HpyF3I CTNAG 5 cut(s) 306, 534, 573, 579, 804
Hsp92II CATG 7 cut(s) 28, 641, 765, 850, 1084, 1163, 1275
Ksp22I TGATCA 2 cut(s) 589, 943
Kzo9I GATC 9 cut(s) 90, 129, 190, 235, 589, 598, 608, 625, 943
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 4 cut(s) 559, 727, 928, 934
MaeI CTAG 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 27, 776, 1010
MalI GATC 9 cut(s) 92, 131, 192, 237, 591, 600, 610, 627, 945
MboI GATC 9 cut(s) 90, 129, 190, 235, 589, 598, 608, 625, 943
MboII GAAGA 6 cut(s) 109, 190, 260, 760, 833, 1046
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 3 cut(s) 207, 874, 1045
MlyI GAGTC 2 cut(s) 317, 1190
MmeI TCCRAC 1 cut(s) 576
Mph1103I ATGCAT 2 cut(s) 1086, 1224
MroXI GAANNNNTTC 1 cut(s) 84
MseI TTAA 2 cut(s) 435, 1248
MslI CAYNNNNRTG 1 cut(s) 258
MspI CCGG 4 cut(s) 207, 968, 1106, 1244
MspR9I CCNGG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 465
NciI CCSGG 1 cut(s) 207
NcoI CCATGG 1 cut(s) 846
NdeII GATC 9 cut(s) 90, 129, 190, 235, 589, 598, 608, 625, 943
NlaIII CATG 7 cut(s) 28, 641, 765, 850, 1084, 1163, 1275
NlaIV GGNNCC 1 cut(s) 966
NmuCI GTSAC 2 cut(s) 776, 1010
NsiI ATGCAT 2 cut(s) 1086, 1224
NspI RCATGY 2 cut(s) 765, 1084
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PdmI GAANNNNTTC 1 cut(s) 84
PfeI GAWTC 4 cut(s) 58, 289, 406, 983
PflFI GACNNNGTC 1 cut(s) 782
PflMI CCANNNNNTGG 1 cut(s) 301
PinAI ACCGGT 2 cut(s) 1105, 1243
PkrI GCNGC 3 cut(s) 7, 416, 458
PleI GAGTC 2 cut(s) 316, 1190
PpsI GAGTC 2 cut(s) 316, 1190
PsiI TTATAA 1 cut(s) 1023
Psp124BI GAGCTC 1 cut(s) 874
PspN4I GGNNCC 1 cut(s) 966
PstNI CAGNNNCTG 2 cut(s) 301, 928
PsuI RGATCY 1 cut(s) 235
PsyI GACNNNGTC 1 cut(s) 782
RsaI GTAC 1 cut(s) 485
RsaNI GTAC 1 cut(s) 484
RseI CAYNNNNRTG 1 cut(s) 258
SacI GAGCTC 1 cut(s) 874
SaqAI TTAA 2 cut(s) 435, 1248
SatI GCNGC 3 cut(s) 6, 415, 457
Sau3AI GATC 9 cut(s) 90, 129, 190, 235, 589, 598, 608, 625, 943
SchI GAGTC 2 cut(s) 317, 1190
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 3 cut(s) 207, 874, 1045
SetI ASST 5 cut(s) 470, 483, 589, 874, 1029
SfaNI GCATC 4 cut(s) 559, 727, 928, 934
SmiMI CAYNNNNRTG 1 cut(s) 258
SmlI CTYRAG 5 cut(s) 312, 351, 469, 503, 1056
SmoI CTYRAG 5 cut(s) 312, 351, 469, 503, 1056
SsiI CCGC 2 cut(s) 414, 459
SspI AATATT 1 cut(s) 347
SspMI CTAG 1 cut(s) 183
SstI GAGCTC 1 cut(s) 874
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 1 cut(s) 846
TaaI ACNGT 2 cut(s) 224, 1127
TaqI TCGA 9 cut(s) 78, 189, 201, 287, 868, 999, 1077, 1089, 1215
TaqII GACCGA 1 cut(s) 1053
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 4 cut(s) 58, 289, 406, 983
Tru1I TTAA 2 cut(s) 435, 1248
Tru9I TTAA 2 cut(s) 435, 1248
TseFI GTSAC 2 cut(s) 776, 1010
TseI GCWGC 2 cut(s) 5, 456
Tsp45I GTSAC 2 cut(s) 776, 1010
TspDTI ATGAA 5 cut(s) 276, 404, 851, 1143, 1255
TspGWI ACGGA 2 cut(s) 372, 812
Tth111I GACNNNGTC 1 cut(s) 782
Van91I CCANNNNNTGG 1 cut(s) 301
XapI RAATTY 2 cut(s) 685, 876
XceI RCATGY 2 cut(s) 765, 1084
XmnI GAANNNNTTC 1 cut(s) 84
XspI CTAG 1 cut(s) 183
Zsp2I ATGCAT 2 cut(s) 1086, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.