Rh4DG410100

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011757
Physical Location & Seq
Reverse (-)
62204532 .. 62209033
4502 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG410100.1

Sequence Viewer

Length: 3189 bp
ATGGCTGCCAACAAGTTTGCCACCATGTTACACAGAAACACCAACAAACTCACTCTGATTCTCATCTACGCAATCCTCGAATGGGTTCTGATCCTCCTCCTCCTTCTCAACTCCCTCTTCTCTTATCTGATCGTCAAGTTCGCCGATTACTTTGGCCTCAAACGACCCTGCCTCTGGTGTTCTAGGGTCGATCACATCTTCGAGCCCGGAAACAAGAGCCACAGTTCTTACAGAGATCTTGTATGTGAAGAACACGCCAATGAAATAGCCAAACTTGGGTTTTGCTCGAATCACCAGAAACTGGCTGAGTCTCAAGATATGTGTGAGGATTGCTCATCGCAGGAATATTCTCAAGAATGGTCTGACAAGTATGCTTTCTTTCCGTGGATGAAGCAAATTGGGATGATTCAAGGCGGCGATGATGATAAGGAGATTAACGATAAGGAATGTTCTTGCTGCGGTGTGAGCTTGAGCAACAAGCTGTACCCTCCTTGTATTCTGCTCAAGCCTTCTTGGGGGGTTTTGGATTACGCTCAGAAACCGATTTTGAGTTCAGATGTAGGGGTTGATGCTCAGACTGAGGAAGGTGATCAGTCGGATCAAAGTGGATCGGATTATGTGGCAGATCAAAATGGACATGATGAGGGTGTTGTTGTTGTTGTTGTTCAAGGAAATAGGGGAGAGAATTCGGTTTCGGTTTCGGTTTCGGATGTTGATGGATGTTGTGGACAAAGAGACTATGATGCAGAGGAGAAGAATAATTCAGCATGTTGTTCTGTTTGTGACTATGTCTGCAAGGAAACGGAAACTGAGGCCAATCAAATTGAAGACGAGGCTAATATGAATGTCTCCATGGACGATGATGGTGATGATGGTCGAGCTCAAATTCAGGCCAGTTGTGATGAGAATAAGTCTCGTGGAAGTCTGCATCAGCATCTGGAGTTCTACATTGATCAAGATGATTGTCGGTTGGTTCCGGTTGAATTGGTTGATTCTCCTGCCACCATCGAGAGGCAAAGTCACCAGAATTATAAGGTGGAGGAAGACCGAGCACATAGTGATACTCAAGATGTTATTCTGGATTTCGACATGCATTTCGAGACACAAGCCAAACCGGTTGTTGAGTTGTGGCACAGTTCAGATGAAATTTTGGAATTACTTTCATCCCATGAGAGTAAAGAGGAGAGCAAAGTTTCAGTTCTTGACTCAAAGGATTTGGATTTCGATATGCATTTCAAGACACAAGCCAAACCGGTTGTTGAGTGTTGGCAGAGTTCAGATGAGATTGTGGAATTACTTTCATCCCATGAGATTAAAGAGGAGAGCAAAGTTTCAGTTCTTGACTCAAAGGATTTGGATTTCGATATGCATTTTGAGACGCAAGCCAAACCAGTTGTTGAGTGTTGTCCCAGTTCAGATGATATTGTGGTACTTTCATCCCATGAGATTAAAGAGGAGAACAAAGTTTCAGTTCTTGACTCAAAGGATTTGGGGCAGAATGGAGAAGAAGTTGAAGAAAATGAGCAAGCTGAAGATACTACTAGTACTCAAGCAACATCAGCCTCTTGTCATGAGGATGACAGTCAATCAAATGCTGCTGACATAGTAGGAAGAGACATTGATTCAGATGTTCATCAAGCAACAACTGAGGATGATGACATCCATATGCGGAACGATGAGATTGATACAGAGGTCTCAATAGGGACTGATATTCCTGATCATGATATATTCAATGAGGATATTCAATCAGCTCATGAACTTCTACATTCTTATCCCAGTGTGCAAGAAGATCCTTCTACAAGTTCTCCCAATGCTGAAATTGATGATAATCATGGTGCTAAGCAAGAAGAAGAGTTTCTAGAATTCAAGACCTTATCAGTTGAAACAAGTGAGAAAGAACAAAATAGTTATTTCTCATTAGGTTCAGAACTTAATGAGATTGAGGAAGAGAAGGTTCTTCCTGATACACCAACTTCTATAGACAATTTCCATCAGTTGTATAATAGAATGCTACTAACTGAGAGAAGGGAATCAGGAACAGAAGCATCGTTGGATGGAAGTGTAGTGAGCGACATTGAATGTGGTGATGGAGTCCTGACTATGGAGAAGCTGAAATCGGTGTTGAGAGCTGAACGGAAAGCTTTAAATAGAGTATATGCAGAACTTGAAGAAGAGAGGAGTGCTTCTGCAATAGCAGCCAACCAGACAATGGCAATGATAAATAGGCTACAAGAAGAAAAGGCAGCAATGCAGATGGAAGCTTTGCAGTACCAAAGGATGATGGATGAGCAGTCTGAGTACGACCAGGAAGCTATGCAGCTCTTAAACGAGCTCATGGTAAAGAGGGAAAAGGAAAAGCAAGAACTTGAAAAGGAGCTAGAAATATGTCGAAAGAAGGTCCAAGATTATGAGGCCAAAGAGAAAATGATGATTTTGAGGAGAATGAAAGAGGGCTGCACAAGAAGTAGGACTTCATCTGGTCCTTGTAGCAATGCTGAGGATAGTGATGGACTTTCTATTGATTTGAATCATGAAGTGAAGGAAGAAGATAACCTCTCGGGACATGAAGAGAGTAGCAATCAAAACACACCAAGGGATGCGGTGTTATATTTAGAAGAATCTTTGGCTAGCTTTGAGGAAGAGAAGTTATCCATTCTAGACCAACTCAAGGAATTGGAAGAGAAGCTTTTGACCTTGAATGACGAAGAGGAAGAAGACCATTACGAGGATATAAAACCAATTGATCATTTCTTTTCAGAGAATGGGAATGGCTACCATGAGAATGGTGATGTTGGCGGTGAAGAAAATGGCATCGCAAATGGCCACTCCAAAGAAATGAATGGAACACATCATCAAGATAGAAAAATTATGGGATCAATTAAGGCAAAGAGACTTCTTCCACTTTTCAATGAAGCTGAAGATGAAGACGAGGAGTTGAATGGAGATGTAGAAGGTTATGATTCTGTTGCATCACAAGATCACAAGAAACTCGCTATTGAAGATGAGGTTGCGCATGTCTATGAGAGGCTACAAGCACTTGAAGCAGATAGAGAGTTTCTAAAGCACTGTATTAGCTCCTTAAGGAAAGGTGACAAGGGATTAGATCTTCTCCAAGAAATTTTGCAACATCTTCGTGACCTGAGAAGTGTGGAAACCCGTCTAAGGAATTCGGGAGAGGGTATCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1062

Amino Acids

120.14

Weight (kDa)

4.41

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 703 - 793 1.1e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1032
Acc16I TGCGCA 1 cut(s) 3012
AccB7I CCANNNNNTGG 2 cut(s) 301, 2209
AciI CCGC 5 cut(s) 414, 459, 1669, 2600, 2796
AclWI GGATC 5 cut(s) 85, 606, 616, 1784, 2881
AcoI YGGCCR 1 cut(s) 2821
AcsI RAATTY 6 cut(s) 685, 885, 1146, 1862, 3117, 3166
AcuI CTGAAG 2 cut(s) 1551, 2937
AdeI CACNNNGTG 1 cut(s) 1058
AfaI GTAC 5 cut(s) 485, 1431, 1546, 2270, 2300
AflII CTTAAG 1 cut(s) 3079
AgeI ACCGGT 2 cut(s) 1114, 1252
AhlI ACTAGT 1 cut(s) 1541
AjnI CCWGG 1 cut(s) 2304
Alw21I GWGCWC 3 cut(s) 883, 1054, 2334
Alw26I GTCTC 9 cut(s) 315, 729, 853, 918, 1094, 1370, 1608, 1699, 2884
AlwI GGATC 5 cut(s) 85, 606, 616, 1784, 2881
AlwNI CAGNNNCTG 2 cut(s) 301, 937
Ama87I CYCGRG 1 cut(s) 2557
AoxI GGCC 5 cut(s) 154, 813, 891, 2412, 2821
ApeKI GCWGC 7 cut(s) 5, 456, 1595, 2195, 2243, 2317, 2454
ApoI RAATTY 6 cut(s) 685, 885, 1146, 1862, 3117, 3166
ArsI GACNNNNNNTTYG 2 cut(s) 1079, 1111
AsiGI ACCGGT 2 cut(s) 1114, 1252
Asp700I GAANNNNTTC 2 cut(s) 84, 1854
AspLEI GCGC 1 cut(s) 3013
AspS9I GGNCC 2 cut(s) 2398, 2480
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 8 cut(s) 284, 599, 878, 1013, 2096, 2798, 2810, 3101
AsuNHI GCTAGC 1 cut(s) 2627
AvaI CYCGRG 1 cut(s) 2557
AvaII GGWCC 2 cut(s) 2398, 2480
BaeI ACNNNNGTAYC 2 cut(s) 1677, 1710
BalI TGGCCA 1 cut(s) 2823
BanII GRGCYC 3 cut(s) 207, 883, 2334
BauI CACGAG 1 cut(s) 915
BbsI GAAGAC 4 cut(s) 834, 1050, 2721, 2931
Bbv12I GWGCWC 3 cut(s) 883, 1054, 2334
BbvCI CCTCAGC 1 cut(s) 2496
BbvI GCAGC 6 cut(s) 443, 1582, 2207, 2255, 2329, 2441
BcgI CGANNNNNNTGC 2 cut(s) 3113, 3147
BciT130I CCWGG 1 cut(s) 2306
BclI TGATCA 4 cut(s) 589, 952, 1717, 2743
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 9 cut(s) 315, 729, 853, 918, 1094, 1370, 1608, 1699, 2884
BcuI ACTAGT 1 cut(s) 1541
BfaI CTAG 6 cut(s) 183, 1542, 1859, 2378, 2628, 2657
BfmI CTRYAG 1 cut(s) 1977
BfrI CTTAAG 1 cut(s) 3079
BglII AGATCT 2 cut(s) 235, 3103
BisI GCNGC 8 cut(s) 6, 415, 457, 1596, 2196, 2244, 2318, 2455
BlpI GCTNAGC 1 cut(s) 1839
BlsI GCNGC 8 cut(s) 7, 416, 458, 1597, 2197, 2245, 2319, 2456
BmcAI AGTACT 1 cut(s) 1546
Bme1390I CCNGG 2 cut(s) 207, 2306
Bme18I GGWCC 2 cut(s) 2398, 2480
BmeT110I CYCGRG 1 cut(s) 2557
BmgT120I GGNCC 2 cut(s) 2398, 2480
BmiI GGNNCC 1 cut(s) 975
BmrFI CCNGG 2 cut(s) 207, 2306
BmrI ACTGGG 2 cut(s) 1404, 1770
BmsI GCATC 8 cut(s) 559, 733, 937, 943, 2054, 2587, 2820, 2978
BmtI GCTAGC 1 cut(s) 2631
BmuI ACTGGG 2 cut(s) 1404, 1770
BpiI GAAGAC 4 cut(s) 834, 1050, 2721, 2931
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 959
Bpu10I CCTNAGC 1 cut(s) 2496
Bpu1102I GCTNAGC 1 cut(s) 1839
BpuEI CTTGAG 7 cut(s) 297, 336, 488, 490, 1050, 1533, 2651
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 4 cut(s) 62, 1632, 1827, 2526
BsaI GGTCTC 1 cut(s) 1699
BsaJI CCNNGG 3 cut(s) 383, 852, 2591
BsaWI WCCGGW 3 cut(s) 976, 1114, 1252
BsaXI ACNNNNNCTCC 4 cut(s) 1494, 1524, 1789, 1819
Bse118I RCCGGY 2 cut(s) 1114, 1252
Bse1I ACTGG 5 cut(s) 306, 894, 1391, 1410, 1776
Bse3DI GCAATG 3 cut(s) 2220, 2253, 2497
Bse8I GATNNNNATC 4 cut(s) 62, 1632, 1827, 2526
BseBI CCWGG 1 cut(s) 2306
BseDI CCNNGG 3 cut(s) 383, 852, 2591
BseJI GATNNNNATC 4 cut(s) 62, 1632, 1827, 2526
BseMI GCAATG 3 cut(s) 2220, 2253, 2497
BseNI ACTGG 5 cut(s) 306, 894, 1391, 1410, 1776
BseRI GAGGAG 9 cut(s) 86, 89, 764, 1196, 1334, 1469, 2191, 2452, 2945
BseXI GCAGC 6 cut(s) 443, 1582, 2207, 2255, 2329, 2441
BsgI GTGCAG 1 cut(s) 2440
BshFI GGCC 5 cut(s) 156, 815, 893, 2414, 2823
BshTI ACCGGT 2 cut(s) 1114, 1252
BsiHKAI GWGCWC 3 cut(s) 883, 1054, 2334
BsiHKCI CYCGRG 1 cut(s) 2557
BsiSI CCGG 4 cut(s) 207, 977, 1115, 1253
BslFI GGGAC 3 cut(s) 1392, 1717, 2574
BsmAI GTCTC 9 cut(s) 315, 729, 853, 918, 1094, 1370, 1608, 1699, 2884
BsmBI CGTCTC 1 cut(s) 1370
BsmFI GGGAC 3 cut(s) 1392, 1717, 2574
BsmI GAATGC 1 cut(s) 2013
BsnI GGCC 5 cut(s) 156, 815, 893, 2414, 2823
Bso31I GGTCTC 1 cut(s) 1699
BsoBI CYCGRG 1 cut(s) 2557
Bsp1286I GDGCHC 4 cut(s) 207, 883, 1054, 2334
Bsp1720I GCTNAGC 1 cut(s) 1839
Bsp19I CCATGG 1 cut(s) 852
BspACI CCGC 5 cut(s) 414, 459, 1669, 2600, 2796
BspANI GGCC 5 cut(s) 156, 815, 893, 2414, 2823
BspHI TCATGA 4 cut(s) 1570, 1720, 1753, 2530
BspLI GGNNCC 1 cut(s) 975
BspOI GCTAGC 1 cut(s) 2631
BspPI GGATC 5 cut(s) 85, 606, 616, 1784, 2881
BspTI CTTAAG 1 cut(s) 3079
BspTNI GGTCTC 1 cut(s) 1699
BsrDI GCAATG 3 cut(s) 2220, 2253, 2497
BsrFI RCCGGY 2 cut(s) 1114, 1252
BsrI ACTGG 5 cut(s) 306, 894, 1391, 1410, 1776
BssAI RCCGGY 2 cut(s) 1114, 1252
BssECI CCNNGG 3 cut(s) 383, 852, 2591
BssSI CACGAG 1 cut(s) 915
BssT1I CCWWGG 2 cut(s) 852, 2591
Bst2BI CACGAG 1 cut(s) 915
Bst2UI CCWGG 1 cut(s) 2306
Bst4CI ACNGT 4 cut(s) 224, 1136, 1583, 3068
Bst6I CTCTTC 9 cut(s) 122, 1606, 1845, 1941, 2166, 2562, 2634, 2673, 2700
BstAFI CTTAAG 1 cut(s) 3079
BstC8I GCNNGC 3 cut(s) 1383, 1527, 2629
BstDSI CCRYGG 2 cut(s) 383, 852
BstHHI GCGC 1 cut(s) 3013
BstMAI GTCTC 9 cut(s) 315, 729, 853, 918, 1094, 1370, 1608, 1699, 2884
BstMWI GCNNNNNNNGC 4 cut(s) 465, 1559, 2195, 3041
BstNI CCWGG 1 cut(s) 2306
BstNSI RCATGY 3 cut(s) 771, 1093, 3017
BstSCI CCNGG 2 cut(s) 205, 2304
BstSFI CTRYAG 1 cut(s) 1977
BstV1I GCAGC 6 cut(s) 443, 1582, 2207, 2255, 2329, 2441
BstV2I GAAGAC 4 cut(s) 834, 1050, 2721, 2931
BstX2I RGATCY 3 cut(s) 235, 1789, 3103
BstXI CCANNNNNNTGG 1 cut(s) 2783
BstYI RGATCY 3 cut(s) 235, 1789, 3103
BsuRI GGCC 5 cut(s) 156, 815, 893, 2414, 2823
BtgI CCRYGG 2 cut(s) 383, 852
BtgZI GCGATG 3 cut(s) 321, 432, 2797
BtsIMutI CAGTG 2 cut(s) 1783, 3064
Cac8I GCNNGC 3 cut(s) 1383, 1527, 2629
CaiI CAGNNNCTG 2 cut(s) 301, 937
CciI TCATGA 4 cut(s) 1570, 1720, 1753, 2530
CfoI GCGC 1 cut(s) 3013
Cfr10I RCCGGY 2 cut(s) 1114, 1252
Cfr13I GGNCC 2 cut(s) 2398, 2480
CseI GACGC 1 cut(s) 1387
Csp6I GTAC 5 cut(s) 484, 1430, 1545, 2269, 2299
CspAI ACCGGT 2 cut(s) 1114, 1252
CviQI GTAC 5 cut(s) 484, 1430, 1545, 2269, 2299
DraI TTTAAA 1 cut(s) 2145
DraIII CACNNNGTG 1 cut(s) 1058
EaeI YGGCCR 1 cut(s) 2821
Eam1104I CTCTTC 9 cut(s) 122, 1606, 1845, 1941, 2166, 2562, 2634, 2673, 2700
EarI CTCTTC 9 cut(s) 122, 1606, 1845, 1941, 2166, 2562, 2634, 2673, 2700
Ecl136II GAGCTC 2 cut(s) 881, 2332
Eco130I CCWWGG 2 cut(s) 852, 2591
Eco24I GRGCYC 3 cut(s) 207, 883, 2334
Eco31I GGTCTC 1 cut(s) 1699
Eco47I GGWCC 2 cut(s) 2398, 2480
Eco53kI GAGCTC 2 cut(s) 881, 2332
Eco57I CTGAAG 2 cut(s) 1551, 2937
Eco88I CYCGRG 1 cut(s) 2557
EcoICRI GAGCTC 2 cut(s) 881, 2332
EcoRI GAATTC 3 cut(s) 685, 1862, 3166
EcoRII CCWGG 1 cut(s) 2304
EcoT14I CCWWGG 2 cut(s) 852, 2591
EcoT22I ATGCAT 3 cut(s) 1095, 1233, 1371
EcoT38I GRGCYC 3 cut(s) 207, 883, 2334
ErhI CCWWGG 2 cut(s) 852, 2591
Esp3I CGTCTC 1 cut(s) 1370
FalI AAGNNNNNCTT 4 cut(s) 2455, 2487, 2670, 2702
FaqI GGGAC 3 cut(s) 1392, 1717, 2574
FauNDI CATATG 1 cut(s) 1665
FbaI TGATCA 4 cut(s) 589, 952, 1717, 2743
Fnu4HI GCNGC 8 cut(s) 6, 415, 457, 1596, 2196, 2244, 2318, 2455
FriOI GRGCYC 3 cut(s) 207, 883, 2334
Fsp4HI GCNGC 8 cut(s) 6, 415, 457, 1596, 2196, 2244, 2318, 2455
FspBI CTAG 6 cut(s) 183, 1542, 1859, 2378, 2628, 2657
FspI TGCGCA 1 cut(s) 3012
GlaI GCGC 1 cut(s) 3012
GluI GCNGC 8 cut(s) 6, 415, 457, 1596, 2196, 2244, 2318, 2455
GsuI CTGGAG 1 cut(s) 959
HaeIII GGCC 5 cut(s) 156, 815, 893, 2414, 2823
HapII CCGG 4 cut(s) 207, 977, 1115, 1253
HgaI GACGC 1 cut(s) 1387
HhaI GCGC 1 cut(s) 3013
Hin6I GCGC 1 cut(s) 3011
HinP1I GCGC 1 cut(s) 3011
HindIII AAGCTT 3 cut(s) 2139, 2259, 2684
HpaII CCGG 4 cut(s) 207, 977, 1115, 1253
HphI GGTGA 8 cut(s) 284, 599, 878, 1013, 2096, 2798, 2810, 3101
Hpy166II GTNNAC 1 cut(s) 728
Hpy8I GTNNAC 1 cut(s) 728
HpyAV CCTTC 9 cut(s) 113, 519, 578, 1803, 1945, 2019, 2389, 2533, 2945
HpyCH4III ACNGT 4 cut(s) 224, 1136, 1583, 3068
HpyF10VI GCNNNNNNNGC 4 cut(s) 465, 1559, 2195, 3041
HspAI GCGC 1 cut(s) 3011
Ksp22I TGATCA 4 cut(s) 589, 952, 1717, 2743
LmnI GCTCC 2 cut(s) 2374, 3080
Lsp1109I GCAGC 6 cut(s) 443, 1582, 2207, 2255, 2329, 2441
LweI GCATC 8 cut(s) 559, 733, 937, 943, 2054, 2587, 2820, 2978
MaeI CTAG 6 cut(s) 183, 1542, 1859, 2378, 2628, 2657
MaeIII GTNAC 5 cut(s) 27, 782, 1019, 3089, 3134
MfeI CAATTG 1 cut(s) 2739
MflI RGATCY 3 cut(s) 235, 1789, 3103
MhlI GDGCHC 4 cut(s) 207, 883, 1054, 2334
MlsI TGGCCA 1 cut(s) 2823
MluNI TGGCCA 1 cut(s) 2823
MlyI GAGTC 5 cut(s) 317, 1199, 1337, 1472, 2100
MmeI TCCRAC 2 cut(s) 576, 2031
Mox20I TGGCCA 1 cut(s) 2823
Mph1103I ATGCAT 3 cut(s) 1095, 1233, 1371
MroXI GAANNNNTTC 2 cut(s) 84, 1854
MscI TGGCCA 1 cut(s) 2823
MseI TTAA 8 cut(s) 435, 1314, 1449, 1932, 2144, 2324, 2880, 3080
MslI CAYNNNNRTG 6 cut(s) 258, 1575, 1664, 2781, 3018, 3132
Msp20I TGGCCA 1 cut(s) 2823
MspCI CTTAAG 1 cut(s) 3079
MspI CCGG 4 cut(s) 207, 977, 1115, 1253
MspR9I CCNGG 2 cut(s) 207, 2306
MunI CAATTG 1 cut(s) 2739
Mva1269I GAATGC 1 cut(s) 2013
MvaI CCWGG 1 cut(s) 2306
MwoI GCNNNNNNNGC 4 cut(s) 465, 1559, 2195, 3041
NciI CCSGG 1 cut(s) 207
NcoI CCATGG 1 cut(s) 852
NdeI CATATG 1 cut(s) 1665
NheI GCTAGC 1 cut(s) 2627
NlaIV GGNNCC 1 cut(s) 975
NmuCI GTSAC 4 cut(s) 782, 1019, 3089, 3134
NsbI TGCGCA 1 cut(s) 3012
NsiI ATGCAT 3 cut(s) 1095, 1233, 1371
NspI RCATGY 3 cut(s) 771, 1093, 3017
PagI TCATGA 4 cut(s) 1570, 1720, 1753, 2530
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 2013
PdmI GAANNNNTTC 2 cut(s) 84, 1854
PfeI GAWTC 9 cut(s) 58, 289, 406, 992, 1622, 2030, 2527, 2618, 2960
PflFI GACNNNGTC 1 cut(s) 788
PflMI CCANNNNNTGG 2 cut(s) 301, 2209
PinAI ACCGGT 2 cut(s) 1114, 1252
PkrI GCNGC 8 cut(s) 7, 416, 458, 1597, 2197, 2245, 2319, 2456
PleI GAGTC 5 cut(s) 316, 1199, 1337, 1472, 2099
PpsI GAGTC 5 cut(s) 316, 1199, 1337, 1472, 2099
PsiI TTATAA 1 cut(s) 1032
Psp124BI GAGCTC 2 cut(s) 883, 2334
Psp6I CCWGG 1 cut(s) 2304
PspGI CCWGG 1 cut(s) 2304
PspN4I GGNNCC 1 cut(s) 975
PspPI GGNCC 2 cut(s) 2398, 2480
PstNI CAGNNNCTG 2 cut(s) 301, 937
PsuI RGATCY 3 cut(s) 235, 1789, 3103
PsyI GACNNNGTC 1 cut(s) 788
RsaI GTAC 5 cut(s) 485, 1431, 1546, 2270, 2300
RsaNI GTAC 5 cut(s) 484, 1430, 1545, 2269, 2299
RseI CAYNNNNRTG 6 cut(s) 258, 1575, 1664, 2781, 3018, 3132
SacI GAGCTC 2 cut(s) 883, 2334
SaqAI TTAA 8 cut(s) 435, 1314, 1449, 1932, 2144, 2324, 2880, 3080
SatI GCNGC 8 cut(s) 6, 415, 457, 1596, 2196, 2244, 2318, 2455
Sau96I GGNCC 2 cut(s) 2398, 2480
ScaI AGTACT 1 cut(s) 1546
SchI GAGTC 5 cut(s) 317, 1199, 1337, 1472, 2100
ScrFI CCNGG 2 cut(s) 207, 2306
SduI GDGCHC 4 cut(s) 207, 883, 1054, 2334
SfaNI GCATC 8 cut(s) 559, 733, 937, 943, 2054, 2587, 2820, 2978
SfcI CTRYAG 1 cut(s) 1977
SinI GGWCC 2 cut(s) 2398, 2480
SmiMI CAYNNNNRTG 6 cut(s) 258, 1575, 1664, 2781, 3018, 3132
SmlI CTYRAG 8 cut(s) 312, 351, 469, 503, 1065, 1548, 2666, 3079
SmoI CTYRAG 8 cut(s) 312, 351, 469, 503, 1065, 1548, 2666, 3079
SpeI ACTAGT 1 cut(s) 1541
SsiI CCGC 5 cut(s) 414, 459, 1669, 2600, 2796
SspI AATATT 1 cut(s) 347
SspMI CTAG 6 cut(s) 183, 1542, 1859, 2378, 2628, 2657
SstI GAGCTC 2 cut(s) 883, 2334
StyD4I CCNGG 2 cut(s) 205, 2304
StyI CCWWGG 2 cut(s) 852, 2591
TaaI ACNGT 4 cut(s) 224, 1136, 1583, 3068
TaqII GACCGA 1 cut(s) 1062
TatI WGTACW 1 cut(s) 1544
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 9 cut(s) 58, 289, 406, 992, 1622, 2030, 2527, 2618, 2960
Tru1I TTAA 8 cut(s) 435, 1314, 1449, 1932, 2144, 2324, 2880, 3080
Tru9I TTAA 8 cut(s) 435, 1314, 1449, 1932, 2144, 2324, 2880, 3080
TscAI CASTG 2 cut(s) 1783, 3071
TseFI GTSAC 4 cut(s) 782, 1019, 3089, 3134
TseI GCWGC 7 cut(s) 5, 456, 1595, 2195, 2243, 2317, 2454
Tsp45I GTSAC 4 cut(s) 782, 1019, 3089, 3134
TspGWI ACGGA 3 cut(s) 372, 818, 2149
TspRI CASTG 2 cut(s) 1783, 3071
Tth111I GACNNNGTC 1 cut(s) 788
Van91I CCANNNNNTGG 2 cut(s) 301, 2209
Vha464I CTTAAG 1 cut(s) 3079
VpaK11BI GGWCC 2 cut(s) 2398, 2480
XapI RAATTY 6 cut(s) 685, 885, 1146, 1862, 3117, 3166
XbaI TCTAGA 2 cut(s) 1858, 2656
XceI RCATGY 3 cut(s) 771, 1093, 3017
XcmI CCANNNNNNNNNTGG 1 cut(s) 2206
XmnI GAANNNNTTC 2 cut(s) 84, 1854
XspI CTAG 6 cut(s) 183, 1542, 1859, 2378, 2628, 2657
ZrmI AGTACT 1 cut(s) 1546
Zsp2I ATGCAT 3 cut(s) 1095, 1233, 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.