FvH4_4g34051

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011757
Physical Location & Seq
Reverse (-)
32031550 .. 32036063
4514 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34051.t1

Sequence Viewer

Length: 3024 bp
ATGGCTGCCAACAAGTTTGCCACCATGTTACACAGAAACACCAACAAACTCACTCTGATTCTCATCTACGCAGTCCTCGAATGGGTTCTGATTCTCCTCCTCCTCCTCAACTCCCTCTTCTCTTATCTGATCGTCAAGTTCGCCGACTACTTTGGCCTCAAACGACCCTGCCTGTGGTGTTCTAGGCTCGATCACATCTTCGAGCCCGGAAACAACAGCCAAAAATCTTACAGAGATCTTGTGTGTGAAGAACACGCCAATGAAATAGCCAAACTTGGGTTCTGCTCCAATCACCAGAAACTGGCGGAGTCTCAAGACATGTGTGAGGATTGCTCATCGCAGCAGGATTGTGATCAAGAATGGTCTAACAAGTATGCTTTCTTTCCTTGGATGAAGCAAATTGGGATGGTTCAAGGCGGCGATGATGATAAAGAGAATTTTGATAAGGAGTGTTCTTGCTGTGGAGTGAGCTTGAGCAGCAAGGTGTACCCTCCTTGTATTCTTCTCAAGCCTTCTTGGGGAGTTTTGGACTACTCTCAAAAACCGATTTTTAATTCCGATGAAAGGGTTGATGCTCAGACTGAGGAAGGTGGTCAGTCGGATGAAACTGGATCGGATCAAAATGGACATGATGACGGTGGTGTTGTTGGAGGAAACAAGGGTAAGAATTCGGTTTCGGATGTTGATGGATGTTGTGGACAAAGAGATTATGACGGTCAGGAGACAAATAATTCAGCTTGTTGTTCTGTTTGTGATTATGTCTGCAAGGAAACTGAAACTGAAGACAAGGCTAGTTTGAATGTGTCCATGGATGGTGATGTTCGAGTTGAGATTCAGGCCAGTTGTGATGAGAACAAGTCATGTGGGAGTCCGCATCAGCATCTGGAGTTCTACATTGATCAAGATGATTGTCGATTGGTGCCGGTTGAATTGGTGGATTCTCCTGCCACCATTGAGAGGCAAAGTCACCAGAATTATAAGGTGGAGGAAGACCAAGCAAATAGTGATACTCAAGATGTTATTCTGGATTTCGACATGCATTTTGAGACACAAGCCAAACCGGTTGTTGAGTGTTGGCACAGTTCAGATGAGATTGAGATTGTGGAATTGCTTTCATCCCATGAGAGTAAAGAGGAGAGTAAAGTTTCAGTTCTTGACTCAAAGGATATGGGTGAGAATGGAGAAGAAAAAGAAATTGAGCAGAATGAAGATGCTACTAGTACTGAAGCAACACCGGCCTCTTGTCATGAGGATAGCAGTCAATCAAATGCTGATATAGTAGGAAGAAAAATTGATTCAGATGTTCATCAAGCCTCTTGCCATGAGGATAGCAGTCAATCAAATGCTGATATAGTAGGAAGAGAAATTGATTCAGATGTTCATCAAGCCTCTTGTCATGAGGATAGCAGTCAATCAAATGCTGATATAGTAGGAAGAGAAATTGATTCAGATGTTCATCAAGCAACAACCGAGGATGATGATATCCATACGCTGAACAACGAGATTGATGCAGAGGTCTCAATTGGGACAGATATTCCTGATCATGATATATTCAATGAGGATATTCAGTCAGCTCATGAACTTCAGAATTCCTATCCCGATGTGCAAGAAGATCCTTCTACAAGTTCTCCGACTACTCCCAATGCTGATAATGATGATAATCATGGTTCTAAGAAAGAAGATGAAGATTTTTTAGAATTCAAGACTTTATCAATTGAAACAAGTGAGAAAGCTCCAAATGGTTATTTCTCATTAGGTTCAGAACTTAATGCGATTGAGGAAGAGAAGGTTTTTCCTGATACCCCAACTTCTATAGACAATTTCCATCAGTTGTATAATAGAATGCTACATACCGAGAGAAGGGATTCAGGAACAGAAGCATCATTGGATGGAAGTGTAATGAGCGATATTGAATGTGGTGATGGAGTCCTAACTATGGAGAAGCTGAAATCGGTGTTGAGAGCTGAACGGGAATCTTTAAATAGAGTATATGCAGAACTTGAAGAAGAGAGGAGTGCTTCTGCAATAGCAGCCAACCAGACAATGGCAATGATAAATAGGCTACAAGAAGAAAAGGCTGCAATGCAGATGGAAGCTTTGCAGTACCAAAGAATGATGGACGAGCAGTCTGAATACGACCAGGAAGCAATGCAGCTCTTAAATGAGCTCATGGTAAAGAGGGAAAAGGAAAAGCAAGAACTTGAAAAGGAGCTGGAAATATGTCGAAAGAAGGTTCAAGATTACGAGGCCAAAGAGAAAATGATGATTTTGAGGAGAATGAAAGAGGGCTGCATAAGAAGTAGGACTTCATCTGGTCCTTGTAGCAATGCTGAGGATAGTGATGGACTGTCGATTGATTTGAATCATGAAGTGAAGGAAGAAGATAACTTATCGGGACATGAAGAAAGTAGCAATCAAAACACACCGAGGGATGCAGTCCTATATTTAGAAGAATCTTTGACTAGCTTTGAGGAAGAGAAGTTATCTATTCTGGACCAACTCAAAGAATTGGAAGAGAAGCTTTTGACCTTGAATGATGAAGAGGAAGAAGAACATTACGAGGATATAAAACCAATCGATCATTTCTTTTCGGAGAATGGAAATGGCTACCATGAGAATGGTGATGTTAGCAGTGAAGAAAATGGCATTGCAAATGGCCACTACAAAGAAATGAATGGAACACATCATCCAGATAGAAAAATTATGGGATCAATCAAGGCAAAGAGACTTCTTCCACTCTTCAATGAAGCTGAAGATGAAGATGAGGAGTTGAATGGAGATGCAGAAGGTTATGATTCTGTTGCATCACAAGAGCACAAGAAACTCGCTATTGAAGAGGAGGTTGATCATGTCTACGAGAGGCTACAAGCACTTGAAGCAGATAGAGAGTTTCTAAAGCATTGCATTAGCTCCTTAAGGAAAGGTGACAAGGGCTTAGATCTTCTACAAGAAATTTTGCAACATCTTCGTGATCTGAGAAGCGTGGAAACCCGTTTAAGGAATTCGGGAGAGGGTATCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1008

Amino Acids

113.62

Weight (kDa)

4.42

Isoelectric Point (pI)

56.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 648 - 738 1.2e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 978
AccB1I GGYRCC 1 cut(s) 919
AccB7I CCANNNNNTGG 2 cut(s) 301, 2044
AccI GTMKAC 1 cut(s) 2853
AciI CCGC 3 cut(s) 305, 417, 872
AclWI GGATC 4 cut(s) 619, 624, 1607, 2716
AcoI YGGCCR 1 cut(s) 2656
AcsI RAATTY 6 cut(s) 436, 667, 1588, 1697, 2952, 3001
AcuI CTGAAG 4 cut(s) 801, 1245, 1568, 2772
AfaI GTAC 3 cut(s) 488, 1222, 2105
AflII CTTAAG 1 cut(s) 2914
AflIII ACRYGT 1 cut(s) 318
AgeI ACCGGT 1 cut(s) 1060
AhdI GACNNNNNGTC 1 cut(s) 2125
AhlI ACTAGT 1 cut(s) 1217
AjnI CCWGG 1 cut(s) 2139
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AloI GAACNNNNNNTCC 2 cut(s) 2671, 2703
Alw21I GWGCWC 2 cut(s) 2169, 2817
Alw26I GTCTC 5 cut(s) 315, 716, 1040, 1522, 2719
AlwI GGATC 4 cut(s) 619, 624, 1607, 2716
AlwNI CAGNNNCTG 2 cut(s) 301, 883
AoxI GGCC 5 cut(s) 154, 837, 1236, 2247, 2656
ApeKI GCWGC 7 cut(s) 5, 340, 477, 2030, 2078, 2152, 2289
ApoI RAATTY 6 cut(s) 436, 667, 1588, 1697, 2952, 3001
ArsI GACNNNNNNTTYG 2 cut(s) 1025, 1057
AsiGI ACCGGT 1 cut(s) 1060
Asp700I GAANNNNTTC 2 cut(s) 84, 1864
AspS9I GGNCC 2 cut(s) 2315, 2494
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 7 cut(s) 284, 827, 959, 1184, 1931, 2633, 2936
AvaII GGWCC 2 cut(s) 2315, 2494
BalI TGGCCA 1 cut(s) 2658
BanI GGYRCC 1 cut(s) 919
BanII GRGCYC 2 cut(s) 207, 2169
BbsI GAAGAC 2 cut(s) 789, 996
Bbv12I GWGCWC 2 cut(s) 2169, 2817
BbvCI CCTCAGC 1 cut(s) 2331
BbvI GCAGC 6 cut(s) 352, 489, 2042, 2065, 2164, 2276
BccI CCATC 9 cut(s) 400, 680, 806, 1833, 1883, 1916, 2083, 2110, 2336
BcgI CGANNNNNNTGC 4 cut(s) 1490, 1524, 2948, 2982
BciT130I CCWGG 1 cut(s) 2141
BclI TGATCA 4 cut(s) 352, 898, 1540, 2845
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 5 cut(s) 315, 716, 1040, 1522, 2719
BcuI ACTAGT 1 cut(s) 1217
BfaI CTAG 4 cut(s) 183, 792, 1218, 2463
BfmI CTRYAG 1 cut(s) 1812
BfrI CTTAAG 1 cut(s) 2914
BglII AGATCT 2 cut(s) 235, 2938
BisI GCNGC 8 cut(s) 6, 341, 418, 478, 2031, 2079, 2153, 2290
BlsI GCNGC 8 cut(s) 7, 342, 419, 479, 2032, 2080, 2154, 2291
BmcAI AGTACT 1 cut(s) 1222
Bme1390I CCNGG 2 cut(s) 207, 2141
Bme18I GGWCC 2 cut(s) 2315, 2494
BmeRI GACNNNNNGTC 1 cut(s) 2125
BmgT120I GGNCC 2 cut(s) 2315, 2494
BmiI GGNNCC 1 cut(s) 921
BmrFI CCNGG 2 cut(s) 207, 2141
BmsI GCATC 9 cut(s) 562, 883, 889, 1201, 1498, 1889, 2422, 2770, 2813
BpiI GAAGAC 2 cut(s) 789, 996
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 1 cut(s) 905
Bpu10I CCTNAGC 1 cut(s) 2331
BpuEI CTTGAG 4 cut(s) 297, 491, 493, 996
BpuMI CCSGG 1 cut(s) 207
Bsa29I ATCGAT 1 cut(s) 2577
BsaBI GATNNNNATC 7 cut(s) 62, 351, 1305, 1380, 1455, 1659, 2361
BsaI GGTCTC 1 cut(s) 1522
BsaJI CCNNGG 4 cut(s) 386, 807, 1470, 2426
BsaWI WCCGGW 1 cut(s) 1060
BsaXI ACNNNNNCTCC 2 cut(s) 1612, 1642
Bse118I RCCGGY 3 cut(s) 922, 1060, 1234
Bse1I ACTGG 3 cut(s) 306, 613, 840
Bse3DI GCAATG 6 cut(s) 2055, 2088, 2154, 2332, 2646, 2899
Bse8I GATNNNNATC 7 cut(s) 62, 351, 1305, 1380, 1455, 1659, 2361
BseBI CCWGG 1 cut(s) 2141
BseCI ATCGAT 1 cut(s) 2577
BseDI CCNNGG 4 cut(s) 386, 807, 1470, 2426
BseJI GATNNNNATC 7 cut(s) 62, 351, 1305, 1380, 1455, 1659, 2361
BseMI GCAATG 6 cut(s) 2055, 2088, 2154, 2332, 2646, 2899
BseMII CTCAG 4 cut(s) 573, 590, 2322, 2966
BseNI ACTGG 3 cut(s) 306, 613, 840
BseRI GAGGAG 9 cut(s) 86, 89, 92, 95, 1148, 2026, 2287, 2780, 2852
BseXI GCAGC 6 cut(s) 352, 489, 2042, 2065, 2164, 2276
BshFI GGCC 5 cut(s) 156, 839, 1238, 2249, 2658
BshNI GGYRCC 1 cut(s) 919
BshTI ACCGGT 1 cut(s) 1060
BshVI ATCGAT 1 cut(s) 2577
BsiHKAI GWGCWC 2 cut(s) 2169, 2817
BsiSI CCGG 4 cut(s) 207, 923, 1061, 1235
BslFI GGGAC 2 cut(s) 1540, 2409
BsmAI GTCTC 5 cut(s) 315, 716, 1040, 1522, 2719
BsmFI GGGAC 2 cut(s) 1540, 2409
BsmI GAATGC 1 cut(s) 1848
BsnI GGCC 5 cut(s) 156, 839, 1238, 2249, 2658
Bso31I GGTCTC 1 cut(s) 1522
Bsp1286I GDGCHC 3 cut(s) 207, 2169, 2817
Bsp19I CCATGG 1 cut(s) 807
BspACI CCGC 3 cut(s) 305, 417, 872
BspANI GGCC 5 cut(s) 156, 839, 1238, 2249, 2658
BspCNI CTCAG 4 cut(s) 574, 589, 2323, 2967
BspDI ATCGAT 1 cut(s) 2577
BspHI TCATGA 5 cut(s) 1246, 1396, 1543, 1576, 2365
BspLI GGNNCC 1 cut(s) 921
BspPI GGATC 4 cut(s) 619, 624, 1607, 2716
BspT107I GGYRCC 1 cut(s) 919
BspTI CTTAAG 1 cut(s) 2914
BspTNI GGTCTC 1 cut(s) 1522
BsrDI GCAATG 6 cut(s) 2055, 2088, 2154, 2332, 2646, 2899
BsrFI RCCGGY 3 cut(s) 922, 1060, 1234
BsrI ACTGG 3 cut(s) 306, 613, 840
BssAI RCCGGY 3 cut(s) 922, 1060, 1234
BssECI CCNNGG 4 cut(s) 386, 807, 1470, 2426
BssT1I CCWWGG 2 cut(s) 386, 807
Bst2UI CCWGG 1 cut(s) 2141
Bst4CI ACNGT 4 cut(s) 638, 716, 1082, 2349
BstAFI CTTAAG 1 cut(s) 2914
BstDEI CTNAG 6 cut(s) 576, 582, 1671, 2331, 2935, 2975
BstDSI CCRYGG 1 cut(s) 807
BstMAI GTCTC 5 cut(s) 315, 716, 1040, 1522, 2719
BstMWI GCNNNNNNNGC 4 cut(s) 477, 1235, 2030, 2876
BstNI CCWGG 1 cut(s) 2141
BstNSI RCATGY 2 cut(s) 322, 1039
BstSCI CCNGG 2 cut(s) 205, 2139
BstSFI CTRYAG 1 cut(s) 1812
BstV1I GCAGC 6 cut(s) 352, 489, 2042, 2065, 2164, 2276
BstV2I GAAGAC 2 cut(s) 789, 996
BstX2I RGATCY 3 cut(s) 235, 1612, 2938
BstXI CCANNNNNNTGG 1 cut(s) 2618
BstYI RGATCY 3 cut(s) 235, 1612, 2938
Bsu15I ATCGAT 1 cut(s) 2577
BsuRI GGCC 5 cut(s) 156, 839, 1238, 2249, 2658
BsuTUI ATCGAT 1 cut(s) 2577
BtgI CCRYGG 1 cut(s) 807
BtgZI GCGATG 2 cut(s) 321, 435
BtsI GCAGTG 1 cut(s) 2638
BtsIMutI CAGTG 1 cut(s) 2638
CaiI CAGNNNCTG 2 cut(s) 301, 883
CciI TCATGA 5 cut(s) 1246, 1396, 1543, 1576, 2365
Cfr10I RCCGGY 3 cut(s) 922, 1060, 1234
Cfr13I GGNCC 2 cut(s) 2315, 2494
ClaI ATCGAT 1 cut(s) 2577
Csp6I GTAC 3 cut(s) 487, 1221, 2104
CspAI ACCGGT 1 cut(s) 1060
CspCI CAANNNNNGTGG 2 cut(s) 844, 879
CviQI GTAC 3 cut(s) 487, 1221, 2104
DdeI CTNAG 6 cut(s) 576, 582, 1671, 2331, 2935, 2975
DraI TTTAAA 1 cut(s) 1980
DriI GACNNNNNGTC 1 cut(s) 2125
EaeI YGGCCR 1 cut(s) 2656
Eam1105I GACNNNNNGTC 1 cut(s) 2125
EciI GGCGGA 1 cut(s) 320
Ecl136II GAGCTC 1 cut(s) 2167
Eco130I CCWWGG 2 cut(s) 386, 807
Eco24I GRGCYC 2 cut(s) 207, 2169
Eco31I GGTCTC 1 cut(s) 1522
Eco32I GATATC 1 cut(s) 1483
Eco47I GGWCC 2 cut(s) 2315, 2494
Eco53kI GAGCTC 1 cut(s) 2167
Eco57I CTGAAG 4 cut(s) 801, 1245, 1568, 2772
EcoICRI GAGCTC 1 cut(s) 2167
EcoRI GAATTC 4 cut(s) 667, 1588, 1697, 3001
EcoRII CCWGG 1 cut(s) 2139
EcoRV GATATC 1 cut(s) 1483
EcoT14I CCWWGG 2 cut(s) 386, 807
EcoT22I ATGCAT 1 cut(s) 1041
EcoT38I GRGCYC 2 cut(s) 207, 2169
ErhI CCWWGG 2 cut(s) 386, 807
FalI AAGNNNNNCTT 4 cut(s) 2290, 2322, 2505, 2537
FaqI GGGAC 2 cut(s) 1540, 2409
FbaI TGATCA 4 cut(s) 352, 898, 1540, 2845
FblI GTMKAC 1 cut(s) 2853
Fnu4HI GCNGC 8 cut(s) 6, 341, 418, 478, 2031, 2079, 2153, 2290
FriOI GRGCYC 2 cut(s) 207, 2169
Fsp4HI GCNGC 8 cut(s) 6, 341, 418, 478, 2031, 2079, 2153, 2290
FspBI CTAG 4 cut(s) 183, 792, 1218, 2463
GluI GCNGC 8 cut(s) 6, 341, 418, 478, 2031, 2079, 2153, 2290
GsuI CTGGAG 1 cut(s) 905
HaeIII GGCC 5 cut(s) 156, 839, 1238, 2249, 2658
HapII CCGG 4 cut(s) 207, 923, 1061, 1235
HindIII AAGCTT 2 cut(s) 2094, 2519
HpaII CCGG 4 cut(s) 207, 923, 1061, 1235
HphI GGTGA 7 cut(s) 284, 827, 959, 1184, 1931, 2633, 2936
Hpy166II GTNNAC 3 cut(s) 487, 698, 2854
Hpy8I GTNNAC 3 cut(s) 487, 698, 2854
HpyAV CCTTC 8 cut(s) 522, 581, 1626, 1780, 1854, 2224, 2368, 2780
HpyCH4III ACNGT 4 cut(s) 638, 716, 1082, 2349
HpyF10VI GCNNNNNNNGC 4 cut(s) 477, 1235, 2030, 2876
HpyF3I CTNAG 6 cut(s) 576, 582, 1671, 2331, 2935, 2975
Ksp22I TGATCA 4 cut(s) 352, 898, 1540, 2845
LmnI GCTCC 4 cut(s) 290, 1738, 2209, 2915
Lsp1109I GCAGC 6 cut(s) 352, 489, 2042, 2065, 2164, 2276
LweI GCATC 9 cut(s) 562, 883, 889, 1201, 1498, 1889, 2422, 2770, 2813
MaeI CTAG 4 cut(s) 183, 792, 1218, 2463
MaeIII GTNAC 3 cut(s) 27, 965, 2924
MfeI CAATTG 2 cut(s) 1521, 1713
MflI RGATCY 3 cut(s) 235, 1612, 2938
MhlI GDGCHC 3 cut(s) 207, 2169, 2817
MlsI TGGCCA 1 cut(s) 2658
MluNI TGGCCA 1 cut(s) 2658
MlyI GAGTC 4 cut(s) 317, 877, 1151, 1935
MmeI TCCRAC 3 cut(s) 579, 628, 1655
Mox20I TGGCCA 1 cut(s) 2658
Mph1103I ATGCAT 1 cut(s) 1041
MroXI GAANNNNTTC 2 cut(s) 84, 1864
MscI TGGCCA 1 cut(s) 2658
MseI TTAA 6 cut(s) 552, 1767, 1979, 2159, 2915, 2996
MslI CAYNNNNRTG 3 cut(s) 258, 2616, 2967
Msp20I TGGCCA 1 cut(s) 2658
MspCI CTTAAG 1 cut(s) 2914
MspI CCGG 4 cut(s) 207, 923, 1061, 1235
MspR9I CCNGG 2 cut(s) 207, 2141
MunI CAATTG 2 cut(s) 1521, 1713
Mva1269I GAATGC 1 cut(s) 1848
MvaI CCWGG 1 cut(s) 2141
MwoI GCNNNNNNNGC 4 cut(s) 477, 1235, 2030, 2876
NciI CCSGG 1 cut(s) 207
NcoI CCATGG 1 cut(s) 807
NlaIV GGNNCC 1 cut(s) 921
NmuCI GTSAC 2 cut(s) 965, 2924
NsiI ATGCAT 1 cut(s) 1041
NspI RCATGY 2 cut(s) 322, 1039
PagI TCATGA 5 cut(s) 1246, 1396, 1543, 1576, 2365
PciI ACATGT 1 cut(s) 318
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 1848
PdmI GAANNNNTTC 2 cut(s) 84, 1864
PflMI CCANNNNNTGG 2 cut(s) 301, 2044
PinAI ACCGGT 1 cut(s) 1060
PkrI GCNGC 8 cut(s) 7, 342, 419, 479, 2032, 2080, 2154, 2291
PleI GAGTC 4 cut(s) 316, 876, 1151, 1934
PpsI GAGTC 4 cut(s) 316, 876, 1151, 1934
PscI ACATGT 1 cut(s) 318
PsiI TTATAA 1 cut(s) 978
Psp124BI GAGCTC 1 cut(s) 2169
Psp6I CCWGG 1 cut(s) 2139
PspGI CCWGG 1 cut(s) 2139
PspN4I GGNNCC 1 cut(s) 921
PspPI GGNCC 2 cut(s) 2315, 2494
PstNI CAGNNNCTG 2 cut(s) 301, 883
PsuI RGATCY 3 cut(s) 235, 1612, 2938
RsaI GTAC 3 cut(s) 488, 1222, 2105
RsaNI GTAC 3 cut(s) 487, 1221, 2104
RseI CAYNNNNRTG 3 cut(s) 258, 2616, 2967
SacI GAGCTC 1 cut(s) 2169
SaqAI TTAA 6 cut(s) 552, 1767, 1979, 2159, 2915, 2996
SatI GCNGC 8 cut(s) 6, 341, 418, 478, 2031, 2079, 2153, 2290
Sau96I GGNCC 2 cut(s) 2315, 2494
ScaI AGTACT 1 cut(s) 1222
SchI GAGTC 4 cut(s) 317, 877, 1151, 1935
ScrFI CCNGG 2 cut(s) 207, 2141
SduI GDGCHC 3 cut(s) 207, 2169, 2817
SfaNI GCATC 9 cut(s) 562, 883, 889, 1201, 1498, 1889, 2422, 2770, 2813
SfcI CTRYAG 1 cut(s) 1812
SinI GGWCC 2 cut(s) 2315, 2494
SmiMI CAYNNNNRTG 3 cut(s) 258, 2616, 2967
SmlI CTYRAG 5 cut(s) 312, 472, 506, 1011, 2914
SmoI CTYRAG 5 cut(s) 312, 472, 506, 1011, 2914
SpeI ACTAGT 1 cut(s) 1217
SsiI CCGC 3 cut(s) 305, 417, 872
SspMI CTAG 4 cut(s) 183, 792, 1218, 2463
SstI GAGCTC 1 cut(s) 2169
StyD4I CCNGG 2 cut(s) 205, 2139
StyI CCWWGG 2 cut(s) 386, 807
TaaI ACNGT 4 cut(s) 638, 716, 1082, 2349
TaqI TCGA 9 cut(s) 78, 189, 201, 823, 913, 1032, 2224, 2351, 2577
TatI WGTACW 1 cut(s) 1220
TauI GCSGC 1 cut(s) 420
Tru1I TTAA 6 cut(s) 552, 1767, 1979, 2159, 2915, 2996
Tru9I TTAA 6 cut(s) 552, 1767, 1979, 2159, 2915, 2996
TscAI CASTG 1 cut(s) 2638
TseFI GTSAC 2 cut(s) 965, 2924
TseI GCWGC 7 cut(s) 5, 340, 477, 2030, 2078, 2152, 2289
Tsp45I GTSAC 2 cut(s) 965, 2924
TspRI CASTG 1 cut(s) 2638
Van91I CCANNNNNTGG 2 cut(s) 301, 2044
Vha464I CTTAAG 1 cut(s) 2914
VpaK11BI GGWCC 2 cut(s) 2315, 2494
XapI RAATTY 6 cut(s) 436, 667, 1588, 1697, 2952, 3001
XceI RCATGY 2 cut(s) 322, 1039
XcmI CCANNNNNNNNNTGG 1 cut(s) 2041
XmiI GTMKAC 1 cut(s) 2853
XmnI GAANNNNTTC 2 cut(s) 84, 1864
XspI CTAG 4 cut(s) 183, 792, 1218, 2463
ZrmI AGTACT 1 cut(s) 1222
Zsp2I ATGCAT 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.