RLG00000005917

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011757
Physical Location & Seq
Forward (+)
2794256 .. 2797515
3260 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005917

Sequence Viewer

Length: 3033 bp
ATGGCTGCCAACAAGTTCGCCACCATGTTACACAGAAACACCAACAAACTCACTCTGATTCTCATCTATGCAATCCTCGAATGGGTTCTGATCCTCCTCCTCCTTCTCAACTCCCTCTTCTCTTATCTGATCGTCAAGTTCGCCGATTACTTTGGCCTCAAACGACCCTGCCTCTGGTGTTCTAGGCTCGATCACATCTTGGAGCCCGGAAACAAGAGCCACAGTTCTTACAGAGATCTTGTATGTGAAGAACACGCCAATGAAATAGCCAAACTTGGGTTTTGCTCGAATCACCAGAAACTGGCTGAGTCTCAAGATATGTGTGAGGATTGCTCATCGCAGGAATATTCTCAAGAATGGTCTAACAAGTACGCTTTCTTTCCGTGGATGAAGCAAATTGGGATGATTCAAGGCGGCGATGATGATAAGGAGGAGATTAACGATAAGGAATGTTCTTGCTGCGGTGTGAGCTTGAGCAACAAGCTGTACCCTCCTTGTATTCTGCTCAAGCCTTCTTGGGGGGTTTTGGATTACGCTCAAAAACCGATTTTGAGTTCAGATGTAGGGGTTGATGCTCAGACTGAGGAAGGTGATCAGTCGGATCAAAGTGGATCGGATTATGTGGCAGATCAAAATGGACATGATGAGGGTGTTGTTGTTGTTCTTGTTGAAGGAAATAGGGCAGAGAATTCGGTTTCGGATGTTGATGGATGTTGTGGACAAAGAGACTACGATGCAGAGGAGAAGAATAATTCAGCATGTTGTTCTGTTTGTGACTATGTCTGCAAGGAAACGGAAACTGAGGCCAATCAAATTGAAGACGAGGCTAATATGAATGTCTCCATGGATGATGGTCGAGCTCAAATTCAGGCCAGTTGTGATGAGAATAAGTCTCGTGGAAGTCTGCATCAGCATCTTGAGTTCTACATTGATCAAGATGATTGTCGGTTGGTTCCGGTTGAATTGGTTGATTCTCCTGCCACCATTGAGAGGCAAAGTCATCAGAATTATAAGGTGGAGGAAGACCGAGCACATAGTGATACTCAAGATGTTATTCTGGATTTCGACATGCATTTCGAGACACAAGCCAAACCGGTTGTTGAGTTGTGGCACAGTTCAGATGAAATTGTGGAATTACTTTCATCCCATGAGAGTAGAGAGGAGAGCAAAGTTTCAGTTCTTGACTCAAAGGATTTGGATTTCGACATGCATTTCAAGACGCAAGCCAAACCGGTTGTTGAGTGTTGGCAGAGTTCAGATGAGATTGTGGAATTACTTTCATCCCATGAGATTAAAGAGGAGAACAAAGTTTCAGTTCTTGACTCAAAGGATTTGGGGCAGAATGGAGAAGAAGTTGAAGAAAATGAGCAAGCTGAAGATACCACTAGTACTCAAGCAACACCAGCCTCTTGTCATGAGGATGACAGTCAATCAAATGCTGCTGATATAGTAGGAAGAGACATTGATTCAGATGTTCATCAAGCAACAACTGAGGATGATGACATCCATATGCGGAACGATGAGATTGATACAGAGGTCTCAATAGGGACTGATATTCCTGATCATGATATATTCAATGAGGATATTCAATCAACTCATGAACTTCTACATTCTTATCCCAGTGTGCAAGAAGATCCTTCTACAAGTTCTCCCAATGCTGAAATTGATGATAATCATGGTGCTAAGCAAGAAGAAGAGTTTCTAGAATTCAAGACCTTATCAGTTGAAACAAGTGAGAAAGAACAAAATAGTTATTTCTCATTAGGTTCAGAACTTAATGAGATTGAGGAAGAGAAGGTTCTTCCTGATACACCAACTTCTATAGACAATTTCAATCAGTTGTATAATAGAATGCTACTAACTGAGAGAAGGGAATCGGGAACAGAAGCATCATTGGATGGAAGTGTAGTGAGCGACATTGAATGTGGTGATGGAGTCCTGACTATGGAGAAGCTGAAATCGGTGTTGAGAGCTGAACGGAAAGCTTTAAATAGAGTATATGCAGAACTCGAAGAAGAGAGGAGTGCTTCTGCAATAGCAGCCAACCAGACAATGGCAATGATAAATAGGCTACAAGAAGAAAAGGCAGCAATGCAGATGGAAGCCTTGCAGTACCAAAGAATGATGGATGAGCAGTCTGAGTACGACCAGGAAGCTATGCAGCTCTTAAACGAGCTCATGGTAAAGAGGGAAAAGGAAAAGCAAGAACTTGAAAAGGAGCTGGAAATATGTCGAAAGAAGGTCCAAGATTATGAGGCCAAAGAGAAAATGATGATCTTGAGGAGAATGAAAGAGGGCTGCACAAGAAGTAGGACTTCATCTGGTCCTTGTAGCAATGCTGAGGATAGTGATGGACTTTCTATTGATTTGAATCATGAAGTGAAGGAAGAAGATAACCTCTCGGGACATGAAGAGAGTAGCAATCAAAACACACCAAGGGATGCGGTGTTATATTTAGAAGAATCTTTGGCTAGCTTTGAGGAAGAGAAGTTATCCATTCTAGACCAACTCAAGGAATTGGAAGAGAAGCTTTTGACCTTGAATGACGAAGAGGAAGAAGAACATTACGAGGATATAAAACCAATTGATCATTTCTTTTCAGAGAATGGGAATGGCTACCATGAGAATGGTGATGTTGGCAGTGAAGAAAATGGCATTGCAAATGGCCACTCCAAAGAAATGAATGGAATACATCATCAAGACAGAAAAATTATGGGATCAATTAAGGCAAAGAGACTTCTTCCACTTTTCAATGAAGCTGAAGATGAAGATGAGGAGTTGAATGGAGATGTAGAAGGTTATGATTCTGTTGCATCACAAGATCACAAGAAACTCGCTATTGAAGAGGAGGTTGCGCATGTCTATGAGAGACTACAAGCACTTGAAGCAGATAGAGAGTTTCTAAAGCACTGTATTAGCTCCTTAAGGAAAGGTGACAAGGGATTAGATCTTCTCCAAGAAATTTTGCAACATCTTCGTGACCTGAGAAGTGTGGAAACCCGTCTAAGGAATTCGGGAGAGGGTATCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Zein-binding

Protein Analysis

1011

Amino Acids

114.58

Weight (kDa)

4.43

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 651 - 741 1e-31 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011757)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G70750
fragaria_vesca FvH4_4g34051
malus_domestica MD13G1021300.v1.1 MD16G1022600.v1.1
prunus_persica Prupe.1G330400_v2.0.a1
pyrus_communis pycom16g01990
rosa_chinensis RchiOBHm_Chr4g0443551
rosa_laevigata RLG00000005917
rosa_multiflora Rmu_co8317059.1_g000001 Rmu_sc0001118.1_g000021 Rmu_sc0001118.1_g000022
rosa_roxburghii Rroxscaffold_5G00384100
rosa_samantha Rh4AG402900 Rh4AG404600 Rh4BG415600 Rh4CG430200 Rh4DG410100 Rh4DG411300
rosa_wichuraiana Rw4G034740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1011
Acc16I TGCGCA 1 cut(s) 2856
AccB7I CCANNNNNTGG 2 cut(s) 301, 2053
AciI CCGC 4 cut(s) 414, 462, 1513, 2444
AclWI GGATC 5 cut(s) 85, 609, 619, 1628, 2725
AcoI YGGCCR 1 cut(s) 2665
AcsI RAATTY 5 cut(s) 688, 864, 1706, 2961, 3010
AcuI CTGAAG 2 cut(s) 1395, 2781
AdeI CACNNNGTG 1 cut(s) 1037
AfaI GTAC 5 cut(s) 371, 488, 1390, 2114, 2144
AflII CTTAAG 1 cut(s) 2923
AgeI ACCGGT 2 cut(s) 1093, 1231
AhlI ACTAGT 1 cut(s) 1385
AjnI CCWGG 1 cut(s) 2148
Alw21I GWGCWC 3 cut(s) 862, 1033, 2178
Alw26I GTCTC 9 cut(s) 315, 720, 844, 897, 1073, 1452, 1543, 2728, 2863
AlwI GGATC 5 cut(s) 85, 609, 619, 1628, 2725
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 1 cut(s) 2401
AoxI GGCC 5 cut(s) 154, 804, 870, 2256, 2665
ApeKI GCWGC 7 cut(s) 5, 459, 1439, 2039, 2087, 2161, 2298
ApoI RAATTY 5 cut(s) 688, 864, 1706, 2961, 3010
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
AsiGI ACCGGT 2 cut(s) 1093, 1231
Asp700I GAANNNNTTC 2 cut(s) 84, 1698
AspLEI GCGC 1 cut(s) 2857
AspS9I GGNCC 2 cut(s) 2242, 2324
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 5 cut(s) 284, 602, 1940, 2642, 2945
AsuNHI GCTAGC 1 cut(s) 2471
AvaI CYCGRG 1 cut(s) 2401
AvaII GGWCC 2 cut(s) 2242, 2324
BaeI ACNNNNGTAYC 2 cut(s) 1521, 1554
BalI TGGCCA 1 cut(s) 2667
BanII GRGCYC 3 cut(s) 207, 862, 2178
BauI CACGAG 1 cut(s) 894
BbsI GAAGAC 2 cut(s) 825, 1029
Bbv12I GWGCWC 3 cut(s) 862, 1033, 2178
BbvCI CCTCAGC 1 cut(s) 2340
BbvI GCAGC 6 cut(s) 446, 1426, 2051, 2099, 2173, 2285
BccI CCATC 7 cut(s) 701, 845, 1892, 1925, 2092, 2119, 2345
BcgI CGANNNNNNTGC 4 cut(s) 672, 706, 2957, 2991
BciT130I CCWGG 1 cut(s) 2150
BclI TGATCA 4 cut(s) 592, 931, 1561, 2587
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 9 cut(s) 315, 720, 844, 897, 1073, 1452, 1543, 2728, 2863
BcuI ACTAGT 1 cut(s) 1385
BfaI CTAG 5 cut(s) 183, 1386, 1703, 2472, 2501
BfmI CTRYAG 1 cut(s) 1821
BfrI CTTAAG 1 cut(s) 2923
BglII AGATCT 2 cut(s) 235, 2947
BisI GCNGC 8 cut(s) 6, 415, 460, 1440, 2040, 2088, 2162, 2299
BlpI GCTNAGC 1 cut(s) 1683
BlsI GCNGC 8 cut(s) 7, 416, 461, 1441, 2041, 2089, 2163, 2300
BmcAI AGTACT 1 cut(s) 1390
Bme1390I CCNGG 2 cut(s) 207, 2150
Bme18I GGWCC 2 cut(s) 2242, 2324
BmeT110I CYCGRG 1 cut(s) 2401
BmgT120I GGNCC 2 cut(s) 2242, 2324
BmiI GGNNCC 2 cut(s) 204, 954
BmrFI CCNGG 2 cut(s) 207, 2150
BmrI ACTGGG 1 cut(s) 1614
BmsI GCATC 7 cut(s) 562, 724, 916, 922, 1898, 2431, 2822
BmtI GCTAGC 1 cut(s) 2475
BmuI ACTGGG 1 cut(s) 1614
BpiI GAAGAC 2 cut(s) 825, 1029
BplI GAGNNNNNCTC 2 cut(s) 317, 349
Bpu10I CCTNAGC 1 cut(s) 2340
Bpu1102I GCTNAGC 1 cut(s) 1683
BpuEI CTTGAG 9 cut(s) 297, 336, 491, 493, 938, 1029, 1377, 2299, 2495
BpuMI CCSGG 1 cut(s) 207
BsaBI GATNNNNATC 4 cut(s) 62, 1476, 1671, 2370
BsaI GGTCTC 1 cut(s) 1543
BsaJI CCNNGG 3 cut(s) 383, 843, 2435
BsaWI WCCGGW 3 cut(s) 955, 1093, 1231
BsaXI ACNNNNNCTCC 4 cut(s) 1338, 1368, 1633, 1663
Bse118I RCCGGY 2 cut(s) 1093, 1231
Bse1I ACTGG 3 cut(s) 306, 873, 1620
Bse3DI GCAATG 4 cut(s) 2064, 2097, 2341, 2655
Bse8I GATNNNNATC 4 cut(s) 62, 1476, 1671, 2370
BseBI CCWGG 1 cut(s) 2150
BseDI CCNNGG 3 cut(s) 383, 843, 2435
BseJI GATNNNNATC 4 cut(s) 62, 1476, 1671, 2370
BseMI GCAATG 4 cut(s) 2064, 2097, 2341, 2655
BseMII CTCAG 9 cut(s) 297, 573, 590, 792, 1482, 1854, 2130, 2331, 2975
BseNI ACTGG 3 cut(s) 306, 873, 1620
BseXI GCAGC 6 cut(s) 446, 1426, 2051, 2099, 2173, 2285
BsgI GTGCAG 1 cut(s) 2284
BshFI GGCC 5 cut(s) 156, 806, 872, 2258, 2667
BshTI ACCGGT 2 cut(s) 1093, 1231
BsiHKAI GWGCWC 3 cut(s) 862, 1033, 2178
BsiHKCI CYCGRG 1 cut(s) 2401
BsiSI CCGG 4 cut(s) 207, 956, 1094, 1232
BslFI GGGAC 2 cut(s) 1561, 2418
BsmAI GTCTC 9 cut(s) 315, 720, 844, 897, 1073, 1452, 1543, 2728, 2863
BsmFI GGGAC 2 cut(s) 1561, 2418
BsmI GAATGC 1 cut(s) 1857
BsnI GGCC 5 cut(s) 156, 806, 872, 2258, 2667
Bso31I GGTCTC 1 cut(s) 1543
BsoBI CYCGRG 1 cut(s) 2401
Bsp1286I GDGCHC 4 cut(s) 207, 862, 1033, 2178
Bsp1720I GCTNAGC 1 cut(s) 1683
Bsp19I CCATGG 1 cut(s) 843
BspACI CCGC 4 cut(s) 414, 462, 1513, 2444
BspANI GGCC 5 cut(s) 156, 806, 872, 2258, 2667
BspCNI CTCAG 9 cut(s) 298, 574, 589, 793, 1483, 1855, 2131, 2332, 2976
BspHI TCATGA 4 cut(s) 1414, 1564, 1597, 2374
BspLI GGNNCC 2 cut(s) 204, 954
BspOI GCTAGC 1 cut(s) 2475
BspPI GGATC 5 cut(s) 85, 609, 619, 1628, 2725
BspTI CTTAAG 1 cut(s) 2923
BspTNI GGTCTC 1 cut(s) 1543
BsrDI GCAATG 4 cut(s) 2064, 2097, 2341, 2655
BsrFI RCCGGY 2 cut(s) 1093, 1231
BsrI ACTGG 3 cut(s) 306, 873, 1620
BssAI RCCGGY 2 cut(s) 1093, 1231
BssECI CCNNGG 3 cut(s) 383, 843, 2435
BssSI CACGAG 1 cut(s) 894
BssT1I CCWWGG 2 cut(s) 843, 2435
Bst2BI CACGAG 1 cut(s) 894
Bst2UI CCWGG 1 cut(s) 2150
Bst4CI ACNGT 4 cut(s) 224, 1115, 1427, 2912
BstAFI CTTAAG 1 cut(s) 2923
BstC8I GCNNGC 3 cut(s) 1224, 1371, 2473
BstDSI CCRYGG 2 cut(s) 383, 843
BstHHI GCGC 1 cut(s) 2857
BstMAI GTCTC 9 cut(s) 315, 720, 844, 897, 1073, 1452, 1543, 2728, 2863
BstMWI GCNNNNNNNGC 4 cut(s) 468, 1403, 2039, 2885
BstNI CCWGG 1 cut(s) 2150
BstNSI RCATGY 4 cut(s) 762, 1072, 1210, 2861
BstSCI CCNGG 2 cut(s) 205, 2148
BstSFI CTRYAG 1 cut(s) 1821
BstV1I GCAGC 6 cut(s) 446, 1426, 2051, 2099, 2173, 2285
BstV2I GAAGAC 2 cut(s) 825, 1029
BstX2I RGATCY 3 cut(s) 235, 1633, 2947
BstXI CCANNNNNNTGG 1 cut(s) 2627
BstYI RGATCY 3 cut(s) 235, 1633, 2947
BsuRI GGCC 5 cut(s) 156, 806, 872, 2258, 2667
BtgI CCRYGG 2 cut(s) 383, 843
BtgZI GCGATG 2 cut(s) 321, 432
BtsI GCAGTG 1 cut(s) 2647
BtsIMutI CAGTG 3 cut(s) 1627, 2647, 2908
Cac8I GCNNGC 3 cut(s) 1224, 1371, 2473
CaiI CAGNNNCTG 1 cut(s) 301
CciI TCATGA 4 cut(s) 1414, 1564, 1597, 2374
CfoI GCGC 1 cut(s) 2857
Cfr10I RCCGGY 2 cut(s) 1093, 1231
Cfr13I GGNCC 2 cut(s) 2242, 2324
CseI GACGC 1 cut(s) 1228
Csp6I GTAC 5 cut(s) 370, 487, 1389, 2113, 2143
CspAI ACCGGT 2 cut(s) 1093, 1231
CviQI GTAC 5 cut(s) 370, 487, 1389, 2113, 2143
DraI TTTAAA 1 cut(s) 1989
DraIII CACNNNGTG 1 cut(s) 1037
EaeI YGGCCR 1 cut(s) 2665
Ecl136II GAGCTC 2 cut(s) 860, 2176
Eco130I CCWWGG 2 cut(s) 843, 2435
Eco24I GRGCYC 3 cut(s) 207, 862, 2178
Eco31I GGTCTC 1 cut(s) 1543
Eco47I GGWCC 2 cut(s) 2242, 2324
Eco53kI GAGCTC 2 cut(s) 860, 2176
Eco57I CTGAAG 2 cut(s) 1395, 2781
Eco88I CYCGRG 1 cut(s) 2401
EcoICRI GAGCTC 2 cut(s) 860, 2176
EcoRI GAATTC 3 cut(s) 688, 1706, 3010
EcoRII CCWGG 1 cut(s) 2148
EcoT14I CCWWGG 2 cut(s) 843, 2435
EcoT22I ATGCAT 2 cut(s) 1074, 1212
EcoT38I GRGCYC 3 cut(s) 207, 862, 2178
ErhI CCWWGG 2 cut(s) 843, 2435
FalI AAGNNNNNCTT 4 cut(s) 2299, 2331, 2514, 2546
FaqI GGGAC 2 cut(s) 1561, 2418
FauNDI CATATG 1 cut(s) 1509
FbaI TGATCA 4 cut(s) 592, 931, 1561, 2587
Fnu4HI GCNGC 8 cut(s) 6, 415, 460, 1440, 2040, 2088, 2162, 2299
FriOI GRGCYC 3 cut(s) 207, 862, 2178
Fsp4HI GCNGC 8 cut(s) 6, 415, 460, 1440, 2040, 2088, 2162, 2299
FspBI CTAG 5 cut(s) 183, 1386, 1703, 2472, 2501
FspI TGCGCA 1 cut(s) 2856
GlaI GCGC 1 cut(s) 2856
GluI GCNGC 8 cut(s) 6, 415, 460, 1440, 2040, 2088, 2162, 2299
HaeIII GGCC 5 cut(s) 156, 806, 872, 2258, 2667
HapII CCGG 4 cut(s) 207, 956, 1094, 1232
HgaI GACGC 1 cut(s) 1228
HhaI GCGC 1 cut(s) 2857
Hin6I GCGC 1 cut(s) 2855
HinP1I GCGC 1 cut(s) 2855
HindIII AAGCTT 2 cut(s) 1983, 2528
HpaII CCGG 4 cut(s) 207, 956, 1094, 1232
HphI GGTGA 5 cut(s) 284, 602, 1940, 2642, 2945
Hpy166II GTNNAC 1 cut(s) 719
Hpy8I GTNNAC 1 cut(s) 719
HpyCH4III ACNGT 4 cut(s) 224, 1115, 1427, 2912
HpyF10VI GCNNNNNNNGC 4 cut(s) 468, 1403, 2039, 2885
HspAI GCGC 1 cut(s) 2855
Ksp22I TGATCA 4 cut(s) 592, 931, 1561, 2587
LmnI GCTCC 3 cut(s) 202, 2218, 2924
Lsp1109I GCAGC 6 cut(s) 446, 1426, 2051, 2099, 2173, 2285
LweI GCATC 7 cut(s) 562, 724, 916, 922, 1898, 2431, 2822
MaeI CTAG 5 cut(s) 183, 1386, 1703, 2472, 2501
MaeIII GTNAC 4 cut(s) 27, 773, 2933, 2978
MfeI CAATTG 1 cut(s) 2583
MflI RGATCY 3 cut(s) 235, 1633, 2947
MhlI GDGCHC 4 cut(s) 207, 862, 1033, 2178
MlsI TGGCCA 1 cut(s) 2667
MluNI TGGCCA 1 cut(s) 2667
MlyI GAGTC 4 cut(s) 317, 1178, 1316, 1944
MmeI TCCRAC 1 cut(s) 579
Mox20I TGGCCA 1 cut(s) 2667
Mph1103I ATGCAT 2 cut(s) 1074, 1212
MroXI GAANNNNTTC 2 cut(s) 84, 1698
MscI TGGCCA 1 cut(s) 2667
MseI TTAA 7 cut(s) 438, 1293, 1776, 1988, 2168, 2724, 2924
MslI CAYNNNNRTG 6 cut(s) 258, 1419, 1508, 2625, 2862, 2976
Msp20I TGGCCA 1 cut(s) 2667
MspCI CTTAAG 1 cut(s) 2923
MspI CCGG 4 cut(s) 207, 956, 1094, 1232
MspR9I CCNGG 2 cut(s) 207, 2150
MunI CAATTG 1 cut(s) 2583
Mva1269I GAATGC 1 cut(s) 1857
MvaI CCWGG 1 cut(s) 2150
MwoI GCNNNNNNNGC 4 cut(s) 468, 1403, 2039, 2885
NciI CCSGG 1 cut(s) 207
NcoI CCATGG 1 cut(s) 843
NdeI CATATG 1 cut(s) 1509
NheI GCTAGC 1 cut(s) 2471
NlaIV GGNNCC 2 cut(s) 204, 954
NmuCI GTSAC 3 cut(s) 773, 2933, 2978
NsbI TGCGCA 1 cut(s) 2856
NsiI ATGCAT 2 cut(s) 1074, 1212
NspI RCATGY 4 cut(s) 762, 1072, 1210, 2861
PagI TCATGA 4 cut(s) 1414, 1564, 1597, 2374
PctI GAATGC 1 cut(s) 1857
PdmI GAANNNNTTC 2 cut(s) 84, 1698
PfeI GAWTC 9 cut(s) 58, 289, 406, 971, 1466, 1874, 2371, 2462, 2804
PflFI GACNNNGTC 1 cut(s) 779
PflMI CCANNNNNTGG 2 cut(s) 301, 2053
PinAI ACCGGT 2 cut(s) 1093, 1231
PkrI GCNGC 8 cut(s) 7, 416, 461, 1441, 2041, 2089, 2163, 2300
PleI GAGTC 4 cut(s) 316, 1178, 1316, 1943
PpsI GAGTC 4 cut(s) 316, 1178, 1316, 1943
PsiI TTATAA 1 cut(s) 1011
Psp124BI GAGCTC 2 cut(s) 862, 2178
Psp6I CCWGG 1 cut(s) 2148
PspGI CCWGG 1 cut(s) 2148
PspN4I GGNNCC 2 cut(s) 204, 954
PspPI GGNCC 2 cut(s) 2242, 2324
PstNI CAGNNNCTG 1 cut(s) 301
PsuI RGATCY 3 cut(s) 235, 1633, 2947
PsyI GACNNNGTC 1 cut(s) 779
RsaI GTAC 5 cut(s) 371, 488, 1390, 2114, 2144
RsaNI GTAC 5 cut(s) 370, 487, 1389, 2113, 2143
RseI CAYNNNNRTG 6 cut(s) 258, 1419, 1508, 2625, 2862, 2976
SacI GAGCTC 2 cut(s) 862, 2178
SaqAI TTAA 7 cut(s) 438, 1293, 1776, 1988, 2168, 2724, 2924
SatI GCNGC 8 cut(s) 6, 415, 460, 1440, 2040, 2088, 2162, 2299
Sau96I GGNCC 2 cut(s) 2242, 2324
ScaI AGTACT 1 cut(s) 1390
SchI GAGTC 4 cut(s) 317, 1178, 1316, 1944
ScrFI CCNGG 2 cut(s) 207, 2150
SduI GDGCHC 4 cut(s) 207, 862, 1033, 2178
SfaNI GCATC 7 cut(s) 562, 724, 916, 922, 1898, 2431, 2822
SfcI CTRYAG 1 cut(s) 1821
SinI GGWCC 2 cut(s) 2242, 2324
SmiMI CAYNNNNRTG 6 cut(s) 258, 1419, 1508, 2625, 2862, 2976
SpeI ACTAGT 1 cut(s) 1385
SsiI CCGC 4 cut(s) 414, 462, 1513, 2444
SspI AATATT 1 cut(s) 347
SspMI CTAG 5 cut(s) 183, 1386, 1703, 2472, 2501
SstI GAGCTC 2 cut(s) 862, 2178
StyD4I CCNGG 2 cut(s) 205, 2148
StyI CCWWGG 2 cut(s) 843, 2435
TaaI ACNGT 4 cut(s) 224, 1115, 1427, 2912
TaqI TCGA 9 cut(s) 78, 189, 287, 856, 1065, 1077, 1203, 2010, 2233
TaqII GACCGA 1 cut(s) 1041
TatI WGTACW 1 cut(s) 1388
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 9 cut(s) 58, 289, 406, 971, 1466, 1874, 2371, 2462, 2804
Tru1I TTAA 7 cut(s) 438, 1293, 1776, 1988, 2168, 2724, 2924
Tru9I TTAA 7 cut(s) 438, 1293, 1776, 1988, 2168, 2724, 2924
TscAI CASTG 3 cut(s) 1627, 2647, 2915
TseFI GTSAC 3 cut(s) 773, 2933, 2978
TseI GCWGC 7 cut(s) 5, 459, 1439, 2039, 2087, 2161, 2298
Tsp45I GTSAC 3 cut(s) 773, 2933, 2978
TspGWI ACGGA 3 cut(s) 372, 809, 1993
TspRI CASTG 3 cut(s) 1627, 2647, 2915
Tth111I GACNNNGTC 1 cut(s) 779
Van91I CCANNNNNTGG 2 cut(s) 301, 2053
Vha464I CTTAAG 1 cut(s) 2923
VpaK11BI GGWCC 2 cut(s) 2242, 2324
XapI RAATTY 5 cut(s) 688, 864, 1706, 2961, 3010
XbaI TCTAGA 2 cut(s) 1702, 2500
XceI RCATGY 4 cut(s) 762, 1072, 1210, 2861
XcmI CCANNNNNNNNNTGG 1 cut(s) 2050
XmnI GAANNNNTTC 2 cut(s) 84, 1698
XspI CTAG 5 cut(s) 183, 1386, 1703, 2472, 2501
ZrmI AGTACT 1 cut(s) 1390
Zsp2I ATGCAT 2 cut(s) 1074, 1212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.