AT1G70950

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005491
Physical Location & Seq
Forward (+)
26752597 .. 26755407
2811 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G70950.1

Sequence Viewer

Length: 1437 bp
ATGGCAGGAGAGATTCAGGACCCGTTTAGTCTTAGTTTTCAGGGGAACTCTATCCACTCTGGATCGATATCCTTTGGAAGATTCGAGAAAGAGGGTTTATCATGGGAGAAGAGGTCATCTTTTTCTCACAATAGGTATCTTGAAGAAGTTGACAAGTGTTCTAAACCTGGCTCTGTTACTGAGATGAAAGCACATTTTGAAGCTCATTTCAAGAAGAAAGGAATCCGATTTCCAGCTTCTCTTGAATCTCAAACTTGGGGAGTTCATCAAACAAGTAATGAGCCTGATGATGAAGCTGTTCATGCTACTGAAAGTTTCGAGGATTATCGATCTGATGGGAGTTTCTCGGAGGATACTAGTCAATCGAATAGTGTTTGTAACTATAGCCATGAACAAGAGAAATGTGGTCAAGGAAAGAGTCAATGTGAGTTTGATGAGGAAAGTGATCATTGTGTTAGCTATGATGAGATTCTAGTGAATTCTGATGAAGTTATTGAGCTTGATGAAGAAGAAGGCGGTGGTGACCACGGGAGGGTTGCTGATCTTGTTGAGTGTGAGAATCTTGGACCTCCTGAGATGCCACAAGAGATTGTGATTCAAGATTCTGCTTTAGTTGAAGAGGCGGGATCGAAACTCGATGAACATGCTTCAAAAAAGCCATCTAATTCTATGGAAACACCCTCCTCCTCTGTAAACGTGAAACCTATTATTCCTAATGATGTGCGTGTGACTAAAGCTTCTACAAAGGGACATGATGTAACGCCAAAAGCTGCTTCAAGGAGGACAAAGGGTTCTTCTTTAAGTTCTAATTCTAAAACCAATGTAGATGCCAAGAGCCAAAAGGAACTAAGACCGAAGAAAACCATTGAGTCTCAACCTAAATCATCGAACAAAACCGAGACTCGACCTCCTATTGCTACGAACAGGTGCAAGACTAGTACCACTTCTTCTAAGCTGGAGATGAGTACAGGTTCTACTAGTTTCCGTTTCAAATGTAGCGAACGAGCTGAGAAGAGAAAAGAGTTCTATATGAAACTGGAGGAGAAAATACATGCTAAGAAAACAGAAACAAACCAAGTTCAGGCCAAAACACAGCAAAAGGCAGAAGCTGAGATTAAACAGTTCAGGAAAAGCCTCAATTTTAAGGCAACACCAATGCCTTCATTCTACAACATCGGTACTAGACCGGTGTCTCATAACAAGACCGAGCCATCTAAAGTAGCACAATCAAGATCACGACCAGCGACCTCAGCCTCAATTACAAACAGGGCGGTCACCAGAGTTTCTTATAAACACGGTTTTGAAGAAGCTGAAATGGTAAAGGTAATGGTCAGTAATAGAAAACAGAGTGCGGCTAAAGATTCAGATTTGCAAAAGGGTAATCTTATGGCGGTTGAAATGAAACAACAGATTGGTGCTCGAAGAAGCAGAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

478

Amino Acids

53.24

Weight (kDa)

7.62

Isoelectric Point (pI)

53.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 328 - 399 1.8e-24 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1290
AciI CCGC 5 cut(s) 516, 623, 1271, 1352, 1391
AclWI GGATC 2 cut(s) 70, 634
AcsI RAATTY 1 cut(s) 478
AfaI GTAC 3 cut(s) 940, 967, 1180
AfiI CCNNNNNNNGG 2 cut(s) 532, 1081
AgeI ACCGGT 1 cut(s) 1186
AhlI ACTAGT 3 cut(s) 356, 935, 977
AjnI CCWGG 1 cut(s) 166
AjuI GAANNNNNNNTTGG 2 cut(s) 757, 789
AloI GAACNNNNNNTCC 2 cut(s) 775, 807
Alw21I GWGCWC 1 cut(s) 1420
Alw26I GTCTC 3 cut(s) 876, 893, 1197
AlwI GGATC 2 cut(s) 70, 634
AlwNI CAGNNNCTG 1 cut(s) 1109
AoxI GGCC 1 cut(s) 1083
ApeKI GCWGC 1 cut(s) 770
ApoI RAATTY 1 cut(s) 478
AsiGI ACCGGT 1 cut(s) 1186
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 2 cut(s) 19, 566
AsuHPI GGTGA 2 cut(s) 533, 1267
AvaII GGWCC 2 cut(s) 19, 566
BarI GAAGNNNNNNTAC 2 cut(s) 931, 963
Bbv12I GWGCWC 1 cut(s) 1420
BbvCI CCTCAGC 1 cut(s) 1249
BbvI GCAGC 1 cut(s) 757
BccI CCATC 3 cut(s) 329, 667, 1219
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 1 cut(s) 168
BciVI GTATCC 1 cut(s) 346
BclI TGATCA 1 cut(s) 445
BcoDI GTCTC 3 cut(s) 876, 893, 1197
BcuI ACTAGT 3 cut(s) 356, 935, 977
BfaI CTAG 6 cut(s) 357, 473, 936, 978, 1182, 1435
BfmI CTRYAG 1 cut(s) 382
BfuI GTATCC 1 cut(s) 346
BisI GCNGC 2 cut(s) 771, 1353
BlsI GCNGC 2 cut(s) 772, 1354
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 19, 566
BmgT120I GGNCC 2 cut(s) 19, 566
BmiI GGNNCC 1 cut(s) 21
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 2 cut(s) 567, 817
BoxI GACNNNNGTC 1 cut(s) 1189
BpmI CTGGAG 2 cut(s) 977, 1058
Bpu10I CCTNAGC 1 cut(s) 1249
Bsa29I ATCGAT 2 cut(s) 65, 328
BsaBI GATNNNNATC 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 526
BsaWI WCCGGW 1 cut(s) 1186
BsaXI ACNNNNNCTCC 4 cut(s) 98, 128, 892, 922
Bsc4I CCNNNNNNNGG 2 cut(s) 532, 1081
Bse118I RCCGGY 1 cut(s) 1186
Bse1I ACTGG 1 cut(s) 1041
Bse8I GATNNNNATC 1 cut(s) 67
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 2 cut(s) 65, 328
BseDI CCNNGG 1 cut(s) 526
BseJI GATNNNNATC 1 cut(s) 67
BseLI CCNNNNNNNGG 2 cut(s) 532, 1081
BseMII CTCAG 5 cut(s) 171, 564, 999, 1101, 1263
BseNI ACTGG 1 cut(s) 1041
BseRI GAGGAG 3 cut(s) 673, 676, 1055
BseXI GCAGC 1 cut(s) 757
BshFI GGCC 1 cut(s) 1085
BshTI ACCGGT 1 cut(s) 1186
BshVI ATCGAT 2 cut(s) 65, 328
BsiHKAI GWGCWC 1 cut(s) 1420
BsiSI CCGG 1 cut(s) 1187
BslFI GGGAC 1 cut(s) 762
BslI CCNNNNNNNGG 2 cut(s) 532, 1081
BsmAI GTCTC 3 cut(s) 876, 893, 1197
BsmFI GGGAC 1 cut(s) 762
BsnI GGCC 1 cut(s) 1085
Bsp1286I GDGCHC 1 cut(s) 1420
Bsp143I GATC 6 cut(s) 62, 329, 445, 541, 626, 1232
BspACI CCGC 5 cut(s) 516, 623, 1271, 1352, 1391
BspANI GGCC 1 cut(s) 1085
BspCNI CTCAG 5 cut(s) 172, 565, 1000, 1102, 1262
BspDI ATCGAT 2 cut(s) 65, 328
BspLI GGNNCC 1 cut(s) 21
BspPI GGATC 2 cut(s) 70, 634
BsrFI RCCGGY 1 cut(s) 1186
BsrI ACTGG 1 cut(s) 1041
BssAI RCCGGY 1 cut(s) 1186
BssECI CCNNGG 1 cut(s) 526
BssMI GATC 6 cut(s) 62, 329, 445, 541, 626, 1232
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 1122, 1298
Bst6I CTCTTC 3 cut(s) 104, 612, 1007
BstDEI CTNAG 9 cut(s) 32, 180, 573, 848, 951, 1008, 1056, 1110, 1249
BstDSI CCRYGG 1 cut(s) 526
BstEII GGTNACC 2 cut(s) 521, 1273
BstKTI GATC 6 cut(s) 65, 332, 448, 544, 629, 1235
BstMAI GTCTC 3 cut(s) 876, 893, 1197
BstMBI GATC 6 cut(s) 62, 329, 445, 541, 626, 1232
BstMWI GCNNNNNNNGC 2 cut(s) 302, 1250
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 2 cut(s) 647, 1055
BstPAI GACNNNNGTC 1 cut(s) 1189
BstPI GGTNACC 2 cut(s) 521, 1273
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 382
BstV1I GCAGC 1 cut(s) 757
Bsu15I ATCGAT 2 cut(s) 65, 328
BsuI GTATCC 1 cut(s) 346
BsuRI GGCC 1 cut(s) 1085
BsuTUI ATCGAT 2 cut(s) 65, 328
BtgI CCRYGG 1 cut(s) 526
CaiI CAGNNNCTG 1 cut(s) 1109
Cfr10I RCCGGY 1 cut(s) 1186
Cfr13I GGNCC 2 cut(s) 19, 566
ClaI ATCGAT 2 cut(s) 65, 328
Csp6I GTAC 3 cut(s) 939, 966, 1179
CspAI ACCGGT 1 cut(s) 1186
CviAII CATG 6 cut(s) 102, 302, 389, 644, 752, 1052
CviQI GTAC 3 cut(s) 939, 966, 1179
DdeI CTNAG 9 cut(s) 32, 180, 573, 848, 951, 1008, 1056, 1110, 1249
DpnI GATC 6 cut(s) 64, 331, 447, 543, 628, 1234
DpnII GATC 6 cut(s) 62, 329, 445, 541, 626, 1232
Eam1104I CTCTTC 3 cut(s) 104, 612, 1007
EarI CTCTTC 3 cut(s) 104, 612, 1007
Eco32I GATATC 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 19, 566
Eco91I GGTNACC 2 cut(s) 521, 1273
EcoO109I RGGNCCY 1 cut(s) 19
EcoO65I GGTNACC 2 cut(s) 521, 1273
EcoRI GAATTC 1 cut(s) 478
EcoRII CCWGG 1 cut(s) 166
EcoRV GATATC 1 cut(s) 69
FaeI CATG 6 cut(s) 105, 305, 392, 647, 755, 1055
FaqI GGGAC 1 cut(s) 762
FatI CATG 6 cut(s) 101, 301, 388, 643, 751, 1051
FauI CCCGC 1 cut(s) 616
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 2 cut(s) 771, 1353
Fsp4HI GCNGC 2 cut(s) 771, 1353
FspBI CTAG 6 cut(s) 357, 473, 936, 978, 1182, 1435
GluI GCNGC 2 cut(s) 771, 1353
GsuI CTGGAG 2 cut(s) 977, 1058
HaeIII GGCC 1 cut(s) 1085
HapII CCGG 1 cut(s) 1187
Hin1II CATG 6 cut(s) 105, 305, 392, 647, 755, 1055
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HindIII AAGCTT 1 cut(s) 735
HpaII CCGG 1 cut(s) 1187
HphI GGTGA 2 cut(s) 533, 1267
Hpy166II GTNNAC 2 cut(s) 151, 694
Hpy188I TCNGA 5 cut(s) 227, 334, 349, 484, 1366
Hpy8I GTNNAC 2 cut(s) 151, 694
HpyAV CCTTC 2 cut(s) 506, 1170
HpyCH4III ACNGT 2 cut(s) 1122, 1298
HpyCH4IV ACGT 1 cut(s) 696
HpyCH4V TGCA 2 cut(s) 930, 1372
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 1250
HpyF3I CTNAG 9 cut(s) 32, 180, 573, 848, 951, 1008, 1056, 1110, 1249
HpySE526I ACGT 1 cut(s) 696
Hsp92II CATG 6 cut(s) 105, 305, 392, 647, 755, 1055
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 6 cut(s) 62, 329, 445, 541, 626, 1232
Lsp1109I GCAGC 1 cut(s) 757
LweI GCATC 2 cut(s) 567, 817
MaeI CTAG 6 cut(s) 357, 473, 936, 978, 1182, 1435
MaeII ACGT 1 cut(s) 696
MaeIII GTNAC 6 cut(s) 175, 377, 521, 727, 757, 1273
MalI GATC 6 cut(s) 64, 331, 447, 543, 628, 1234
MboI GATC 6 cut(s) 62, 329, 445, 541, 626, 1232
MhlI GDGCHC 1 cut(s) 1420
MluCI AATT 5 cut(s) 478, 664, 808, 1138, 1257
MlyI GAGTC 3 cut(s) 427, 878, 895
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 3 cut(s) 800, 1116, 1143
MspI CCGG 1 cut(s) 1187
MspR9I CCNGG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 302, 1250
NdeII GATC 6 cut(s) 62, 329, 445, 541, 626, 1232
NlaIII CATG 6 cut(s) 105, 305, 392, 647, 755, 1055
NlaIV GGNNCC 1 cut(s) 21
NmuCI GTSAC 3 cut(s) 521, 727, 1273
NspI RCATGY 2 cut(s) 647, 1055
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 9 cut(s) 13, 81, 222, 245, 469, 559, 595, 602, 1361
PinAI ACCGGT 1 cut(s) 1186
PkrI GCNGC 2 cut(s) 772, 1354
PleI GAGTC 3 cut(s) 426, 877, 895
PpsI GAGTC 3 cut(s) 426, 877, 895
PpuMI RGGWCCY 1 cut(s) 19
PshAI GACNNNNGTC 1 cut(s) 1189
PsiI TTATAA 1 cut(s) 1290
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 1 cut(s) 166
PspEI GGTNACC 2 cut(s) 521, 1273
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 2 cut(s) 19, 566
PspPPI RGGWCCY 1 cut(s) 19
PstNI CAGNNNCTG 1 cut(s) 1109
RsaI GTAC 3 cut(s) 940, 967, 1180
RsaNI GTAC 3 cut(s) 939, 966, 1179
SaqAI TTAA 3 cut(s) 800, 1116, 1143
SatI GCNGC 2 cut(s) 771, 1353
Sau3AI GATC 6 cut(s) 62, 329, 445, 541, 626, 1232
Sau96I GGNCC 2 cut(s) 19, 566
SchI GAGTC 3 cut(s) 427, 878, 895
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 1420
SfaNI GCATC 2 cut(s) 567, 817
SfcI CTRYAG 1 cut(s) 382
SinI GGWCC 2 cut(s) 19, 566
SpeI ACTAGT 3 cut(s) 356, 935, 977
Sse9I AATT 5 cut(s) 478, 664, 808, 1138, 1257
SsiI CCGC 5 cut(s) 516, 623, 1271, 1352, 1391
SspMI CTAG 6 cut(s) 357, 473, 936, 978, 1182, 1435
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 1122, 1298
TaiI ACGT 1 cut(s) 699
TaqII GACCGA 2 cut(s) 868, 1220
TasI AATT 5 cut(s) 478, 664, 808, 1138, 1257
TatI WGTACW 1 cut(s) 965
TauI GCSGC 1 cut(s) 1355
TfiI GAWTC 9 cut(s) 13, 81, 222, 245, 469, 559, 595, 602, 1361
Tru1I TTAA 3 cut(s) 800, 1116, 1143
Tru9I TTAA 3 cut(s) 800, 1116, 1143
TseFI GTSAC 3 cut(s) 521, 727, 1273
TseI GCWGC 1 cut(s) 770
Tsp45I GTSAC 3 cut(s) 521, 727, 1273
TspGWI ACGGA 1 cut(s) 974
VpaK11BI GGWCC 2 cut(s) 19, 566
XapI RAATTY 1 cut(s) 478
XceI RCATGY 2 cut(s) 647, 1055
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 6 cut(s) 357, 473, 936, 978, 1182, 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.