Rh4AG384000

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0005491
Physical Location & Seq
Reverse (-)
69033231 .. 69037238
4008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG384000.1

Sequence Viewer

Length: 1470 bp
ATGGCTGGGGAAATAGAAGAACCCTTTTGTTTTAGCTTTCAGCCAAAGTCTATACATTCTGGCTCCATATCATTTGGGAGGTTTGAAAACGAAGGCTTATCTTGGGAAAGGAGGTCTGCTTTCTCACATAACAGATATGTTGAAGAGGTTGAGAAATGCATGAAACCAGGTTCAGTTATTGAGAGGAAAGCTTATTTTGAAGCTCATTTTAAGAAGGGTCTTCCCAGACCAAATTTAGCCGAGTGTTATAGAGGTACATGCTACCAAGTCGGCGAGAATGATGTATCAGAGAGTGATGACGCTTACAGAGAAGAATGTGAACTTGAAAATGAAGGGAGCAGTTATGCTGAATCAGATGACCAAAGGTCGGAGGGTTCTGTGTATCTTGGAGATTTTGAAGTCACAGAATGTGAAAAAGAAGAAGATACTGAACTTCCGTCCTCTGACCCTCAGAGGGCACCTGCATTGAATCATATTGCTATTTTGGTGGATGGTGCTGTTGAAGATGTTAATTTTGAGGAAGCGCATCAACCCGAGAAAGGTTGTGCAAAATCTACTTCGATTGTTGAGGAACTGGAGAGAGATGCAAATGAGAATCATAATGGTGATGCAGTGAATGCTGAGGAGCCGCCTAAGAGTGTTGATATATGTTCAAATACTGAACCAGCTGAGTGTGTTGATAAAACCATTGTGGAAAGTGGGAAAAGTACTTCTTCAAAGTTGAAGGCTGTAAAAGAATCCAAATTTGTAAAGCCTAAGGCCAGCATTTCTCAGGTCCAAAGAAACATTTCTGCTAAGGGATCTGGTATATATCCTATAGAGAATTCCAATACTGGACAAAGAGAGCGTACACGAAGAACGGGTAAAGAAAAGCTCCCATTAAAGACTGCAATGCCAACTAATAAGCATTCTGTTCGCAGAACTCCGAAGTTAGAGGACTCCATGAAGGGAAAATCTTTAAATGAGAGTAAAAGTGTTAAAGAGTCATCACTTAAGAAGCTTGGTGAACTTCAACCTTCTGCATTCAAGCCTGAATCCAGAGGACGCCAAATGACAAACAGGTATAGGTTCCATCAGAATTTTCCCATTGAATGCTATTTGTGTTTCAATTTTGGAACTGTTGAATTTTTATCAAGTCAAGTCACTTTTCGTACCTTCTATAGGGTTAGTAACGGAGCCAATTTGACTAAGTCTGATGCAAGATCAAGTGTTACAACTTTCAAATTCAAAAGTTCTGAGCGAGCAGAAAAGAGGAAAGAGTTCTTTATGAAGTTGGAAGAAAAAATGCACGCTAAGGAAGATGAAATGAACAAGGTCCAAGCAAGAAAACAGGAAAGGATAGAAGCTGAGACGAAGCAATTCCGGAAAGCCCTCAACTTCAAAGCAGCACCAATGCCTTCTTTCTACAATGTAGCTAGCGCTTCGAGGCCTGATGGGAGCAAGGTAAATAAATTCATTGCATTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

489

Amino Acids

55.31

Weight (kDa)

6.57

Isoelectric Point (pI)

51.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 407 - 472 7.3e-21 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 469
Acc36I ACCTGC 1 cut(s) 469
AccB1I GGYRCC 1 cut(s) 457
AccIII TCCGGA 1 cut(s) 1362
AciI CCGC 1 cut(s) 629
AclWI GGATC 1 cut(s) 808
AcsI RAATTY 7 cut(s) 232, 743, 823, 1078, 1124, 1223, 1451
AcyI GRCGYC 1 cut(s) 1045
AfaI GTAC 4 cut(s) 256, 709, 850, 1153
AfeI AGCGCT 1 cut(s) 1420
AfiI CCNNNNNNNGG 4 cut(s) 367, 454, 539, 1161
AflII CTTAAG 1 cut(s) 992
AhdI GACNNNNNGTC 1 cut(s) 364
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 8 cut(s) 36, 191, 203, 668, 874, 1000, 1346, 1415
AluI AGCT 8 cut(s) 36, 191, 203, 668, 874, 1000, 1346, 1415
Alw26I GTCTC 1 cut(s) 1343
AlwI GGATC 1 cut(s) 808
Ama87I CYCGRG 1 cut(s) 533
Aor13HI TCCGGA 1 cut(s) 1362
Aor51HI AGCGCT 1 cut(s) 1420
AoxI GGCC 2 cut(s) 759, 1427
ApeKI GCWGC 1 cut(s) 1385
ApoI RAATTY 7 cut(s) 232, 743, 823, 1078, 1124, 1223, 1451
Asp700I GAANNNNTTC 3 cut(s) 787, 1259, 1358
AspLEI GCGC 2 cut(s) 526, 1421
AspS9I GGNCC 2 cut(s) 775, 1315
AsuHPI GGTGA 2 cut(s) 617, 1016
AsuNHI GCTAGC 1 cut(s) 1415
AvaI CYCGRG 1 cut(s) 533
AvaII GGWCC 2 cut(s) 775, 1315
AxyI CCTNAGG 1 cut(s) 756
BaeGI GKGCMC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 212
BbvCI CCTCAGC 1 cut(s) 621
BbvI GCAGC 1 cut(s) 1397
BccI CCATC 3 cut(s) 485, 1080, 1427
BcgI CGANNNNNNTGC 2 cut(s) 896, 930
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 1343
BfaI CTAG 1 cut(s) 1416
BfmI CTRYAG 2 cut(s) 816, 1159
BfoI RGCGCY 1 cut(s) 1422
BfrI CTTAAG 1 cut(s) 992
BfuAI ACCTGC 1 cut(s) 469
BisI GCNGC 2 cut(s) 629, 1386
BlsI GCNGC 2 cut(s) 630, 1387
BmcAI AGTACT 1 cut(s) 709
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 775, 1315
BmeRI GACNNNNNGTC 1 cut(s) 364
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 2 cut(s) 775, 1315
BmiI GGNNCC 5 cut(s) 64, 459, 627, 1070, 1177
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 4 cut(s) 535, 574, 598, 1186
BmtI GCTAGC 1 cut(s) 1419
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 596
Bpu10I CCTNAGC 3 cut(s) 621, 795, 1293
BsaHI GRCGYC 1 cut(s) 1045
BsaWI WCCGGW 1 cut(s) 1362
Bsc4I CCNNNNNNNGG 4 cut(s) 367, 454, 539, 1161
Bse1I ACTGG 2 cut(s) 579, 838
Bse21I CCTNAGG 1 cut(s) 756
Bse3DI GCAATG 2 cut(s) 897, 1455
BseAI TCCGGA 1 cut(s) 1362
BseBI CCWGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 4 cut(s) 367, 454, 539, 1161
BseMI GCAATG 2 cut(s) 897, 1455
BseMII CTCAG 6 cut(s) 464, 612, 660, 785, 1227, 1338
BseNI ACTGG 2 cut(s) 579, 838
BseRI GAGGAG 1 cut(s) 638
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 1 cut(s) 1397
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 2 cut(s) 761, 1429
BshNI GGYRCC 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 1 cut(s) 1363
BslI CCNNNNNNNGG 4 cut(s) 367, 454, 539, 1161
BsmAI GTCTC 1 cut(s) 1343
BsmBI CGTCTC 1 cut(s) 1343
BsmI GAATGC 4 cut(s) 622, 907, 1022, 1097
BsnI GGCC 2 cut(s) 761, 1429
BsoBI CYCGRG 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 460
Bsp13I TCCGGA 1 cut(s) 1362
Bsp143I GATC 2 cut(s) 800, 1202
BspACI CCGC 1 cut(s) 629
BspANI GGCC 2 cut(s) 761, 1429
BspCNI CTCAG 6 cut(s) 463, 613, 661, 784, 1228, 1339
BspEI TCCGGA 1 cut(s) 1362
BspLI GGNNCC 5 cut(s) 64, 459, 627, 1070, 1177
BspMI ACCTGC 1 cut(s) 469
BspOI GCTAGC 1 cut(s) 1419
BspPI GGATC 1 cut(s) 808
BspT107I GGYRCC 1 cut(s) 457
BspTI CTTAAG 1 cut(s) 992
BsrDI GCAATG 2 cut(s) 897, 1455
BsrI ACTGG 2 cut(s) 579, 838
BssMI GATC 2 cut(s) 800, 1202
BssNI GRCGYC 1 cut(s) 1045
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 1 cut(s) 1120
Bst6I CTCTTC 1 cut(s) 138
BstACI GRCGYC 1 cut(s) 1045
BstAFI CTTAAG 1 cut(s) 992
BstAPI GCANNNNNTGC 1 cut(s) 617
BstC8I GCNNGC 4 cut(s) 763, 1242, 1290, 1417
BstENI CCTNNNNNAGG 1 cut(s) 1159
BstF5I GGATG 1 cut(s) 496
BstH2I RGCGCY 1 cut(s) 1422
BstHHI GCGC 2 cut(s) 526, 1421
BstKTI GATC 2 cut(s) 803, 1205
BstMAI GTCTC 1 cut(s) 1343
BstMBI GATC 2 cut(s) 800, 1202
BstMWI GCNNNNNNNGC 1 cut(s) 617
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 2 cut(s) 816, 1159
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 1 cut(s) 1397
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 1 cut(s) 800
BstYI RGATCY 1 cut(s) 800
Bsu36I CCTNAGG 1 cut(s) 756
BsuRI GGCC 2 cut(s) 761, 1429
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 1 cut(s) 618
BveI ACCTGC 1 cut(s) 469
Cac8I GCNNGC 4 cut(s) 763, 1242, 1290, 1417
CfoI GCGC 2 cut(s) 526, 1421
Cfr13I GGNCC 2 cut(s) 775, 1315
CseI GACGC 2 cut(s) 308, 1053
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 4 cut(s) 255, 708, 849, 1152
CviAII CATG 3 cut(s) 160, 258, 943
CviQI GTAC 4 cut(s) 255, 708, 849, 1152
DpnI GATC 2 cut(s) 802, 1204
DpnII GATC 2 cut(s) 800, 1202
DraI TTTAAA 1 cut(s) 960
DriI GACNNNNNGTC 1 cut(s) 364
Eam1104I CTCTTC 1 cut(s) 138
Eam1105I GACNNNNNGTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 138
Eco147I AGGCCT 1 cut(s) 1429
Eco47I GGWCC 2 cut(s) 775, 1315
Eco47III AGCGCT 1 cut(s) 1420
Eco81I CCTNAGG 1 cut(s) 756
Eco88I CYCGRG 1 cut(s) 533
EcoNI CCTNNNNNAGG 1 cut(s) 1159
EcoRI GAATTC 1 cut(s) 823
EcoRII CCWGG 1 cut(s) 166
EcoT22I ATGCAT 1 cut(s) 161
Esp3I CGTCTC 1 cut(s) 1343
FaeI CATG 3 cut(s) 163, 261, 946
FalI AAGNNNNNCTT 2 cut(s) 697, 729
FatI CATG 3 cut(s) 159, 257, 942
Fnu4HI GCNGC 2 cut(s) 629, 1386
FokI GGATG 1 cut(s) 503
Fsp4HI GCNGC 2 cut(s) 629, 1386
FspBI CTAG 1 cut(s) 1416
GlaI GCGC 2 cut(s) 525, 1420
GluI GCNGC 2 cut(s) 629, 1386
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 596
HaeII RGCGCY 1 cut(s) 1422
HaeIII GGCC 2 cut(s) 761, 1429
HapII CCGG 1 cut(s) 1363
HgaI GACGC 2 cut(s) 308, 1053
HhaI GCGC 2 cut(s) 526, 1421
Hin1I GRCGYC 1 cut(s) 1045
Hin1II CATG 3 cut(s) 163, 261, 946
Hin6I GCGC 2 cut(s) 524, 1419
HinP1I GCGC 2 cut(s) 524, 1419
HindIII AAGCTT 2 cut(s) 189, 998
HinfI GANTC 7 cut(s) 350, 469, 595, 737, 938, 983, 1034
HpaII CCGG 1 cut(s) 1363
HphI GGTGA 2 cut(s) 617, 1016
Hpy166II GTNNAC 3 cut(s) 320, 851, 1007
Hpy188I TCNGA 9 cut(s) 289, 355, 370, 445, 453, 927, 1077, 1195, 1237
Hpy188III TCNNGA 2 cut(s) 1038, 1363
Hpy8I GTNNAC 3 cut(s) 320, 851, 1007
HpyAV CCTTC 8 cut(s) 86, 208, 326, 718, 940, 1026, 1165, 1407
HpyCH4III ACNGT 1 cut(s) 1120
HpyF10VI GCNNNNNNNGC 1 cut(s) 617
Hsp92I GRCGYC 1 cut(s) 1045
Hsp92II CATG 3 cut(s) 163, 261, 946
HspAI GCGC 2 cut(s) 524, 1419
Kpn2I TCCGGA 1 cut(s) 1362
Kzo9I GATC 2 cut(s) 800, 1202
LmnI GCTCC 6 cut(s) 68, 336, 625, 879, 1175, 1437
Lsp1109I GCAGC 1 cut(s) 1397
LweI GCATC 4 cut(s) 535, 574, 598, 1186
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 1 cut(s) 1416
MaeIII GTNAC 4 cut(s) 400, 1141, 1169, 1210
MalI GATC 2 cut(s) 802, 1204
MboI GATC 2 cut(s) 800, 1202
MflI RGATCY 1 cut(s) 800
MhlI GDGCHC 1 cut(s) 460
MlyI GAGTC 2 cut(s) 932, 992
MmeI TCCRAC 2 cut(s) 348, 1254
Mph1103I ATGCAT 1 cut(s) 161
MroI TCCGGA 1 cut(s) 1362
MroXI GAANNNNTTC 3 cut(s) 787, 1259, 1358
MseI TTAA 8 cut(s) 210, 510, 881, 959, 978, 993, 1464, 1468
MslI CAYNNNNRTG 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 668
MspCI CTTAAG 1 cut(s) 992
MspI CCGG 1 cut(s) 1363
MspR9I CCNGG 1 cut(s) 168
Mva1269I GAATGC 4 cut(s) 622, 907, 1022, 1097
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 617
NdeII GATC 2 cut(s) 800, 1202
NheI GCTAGC 1 cut(s) 1415
NlaIII CATG 3 cut(s) 163, 261, 946
NlaIV GGNNCC 5 cut(s) 64, 459, 627, 1070, 1177
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 2 cut(s) 400, 1141
NsiI ATGCAT 1 cut(s) 161
NspI RCATGY 1 cut(s) 261
PacI TTAATTAA 1 cut(s) 1468
PaqCI CACCTGC 1 cut(s) 469
PceI AGGCCT 1 cut(s) 1429
PctI GAATGC 4 cut(s) 622, 907, 1022, 1097
PdmI GAANNNNTTC 3 cut(s) 787, 1259, 1358
PfeI GAWTC 5 cut(s) 350, 469, 595, 737, 1034
PflFI GACNNNGTC 1 cut(s) 1189
PkrI GCNGC 2 cut(s) 630, 1387
PleI GAGTC 2 cut(s) 932, 991
PpsI GAGTC 2 cut(s) 932, 991
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 5 cut(s) 64, 459, 627, 1070, 1177
PspPI GGNCC 2 cut(s) 775, 1315
PsuI RGATCY 1 cut(s) 800
PsyI GACNNNGTC 1 cut(s) 1189
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 4 cut(s) 256, 709, 850, 1153
RsaNI GTAC 4 cut(s) 255, 708, 849, 1152
RseI CAYNNNNRTG 1 cut(s) 603
SaqAI TTAA 8 cut(s) 210, 510, 881, 959, 978, 993, 1464, 1468
SatI GCNGC 2 cut(s) 629, 1386
Sau3AI GATC 2 cut(s) 800, 1202
Sau96I GGNCC 2 cut(s) 775, 1315
ScaI AGTACT 1 cut(s) 709
SchI GAGTC 2 cut(s) 932, 992
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 460
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 4 cut(s) 535, 574, 598, 1186
SfcI CTRYAG 2 cut(s) 816, 1159
SinI GGWCC 2 cut(s) 775, 1315
SmiMI CAYNNNNRTG 1 cut(s) 603
SmlI CTYRAG 1 cut(s) 992
SmoI CTYRAG 1 cut(s) 992
SseBI AGGCCT 1 cut(s) 1429
SsiI CCGC 1 cut(s) 629
SspMI CTAG 1 cut(s) 1416
StuI AGGCCT 1 cut(s) 1429
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 1 cut(s) 1120
TaqI TCGA 2 cut(s) 560, 1424
TatI WGTACW 1 cut(s) 707
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 5 cut(s) 350, 469, 595, 737, 1034
Tru1I TTAA 8 cut(s) 210, 510, 881, 959, 978, 993, 1464, 1468
Tru9I TTAA 8 cut(s) 210, 510, 881, 959, 978, 993, 1464, 1468
TscAI CASTG 1 cut(s) 618
TseFI GTSAC 2 cut(s) 400, 1141
TseI GCWGC 1 cut(s) 1385
Tsp45I GTSAC 2 cut(s) 400, 1141
TspDTI ATGAA 7 cut(s) 176, 345, 959, 1283, 1317, 1322, 1444
TspGWI ACGGA 2 cut(s) 426, 1188
TspRI CASTG 1 cut(s) 618
Tth111I GACNNNGTC 1 cut(s) 1189
Vha464I CTTAAG 1 cut(s) 992
VpaK11BI GGWCC 2 cut(s) 775, 1315
XagI CCTNNNNNAGG 1 cut(s) 1159
XapI RAATTY 7 cut(s) 232, 743, 823, 1078, 1124, 1223, 1451
XceI RCATGY 1 cut(s) 261
XmnI GAANNNNTTC 3 cut(s) 787, 1259, 1358
XspI CTAG 1 cut(s) 1416
ZrmI AGTACT 1 cut(s) 709
Zsp2I ATGCAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.