Rh4CG413200

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005491
Physical Location & Seq
Reverse (-)
65788192 .. 65792257
4066 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG413200.1

Sequence Viewer

Length: 1371 bp
ATGGCTGGGGAAATAGAAGAACCCTTTTGTTTTAGCTTTCAGCCAAAGTCTATACATTCTGGCTCCATATCATTTGGGAGGTTCGAAAACGAAGGCTTATCTTGGGAAAGGAGGTCTGCTTTCTCACATAACAGATATGTTGAAGAGGTTGAGAAATGCATGAAACCAGGTTCAGTTATTGAGAGGAAAGCTTATTTTGAAGCTCATTTTAAGAAGAAGGGTCTTCCCAGACCAAATTTAGCCGAGTGTTATAGTGGTACATGCTACCAAGTCGGCGAGAATGATGTATCAGAGAGTGATGACGCTTACAGAGAAGAATGTGAACTTGAAAATGAAGGGAGCAGTTATGCTGAATCAGATGACCAAAGGTCGGAGGGTTCTGTGTATCTTGGAGATTTTGAAGTCACAGAACGTGAAAAAGAAGAAGATACTGAACTTCCGTCCTCTGACCCTCAGAGGGCACCTGCATTGAATCATATTGCTATTTTGGTGGATGGTGCTGTTGAAGATGTTGATTTTGAGGAAGCGCATCAACCCGAGAAAGGTTGTGCAAAATCTACTTCGATTGTTGAGGAACTGGAGAGAGATGCAAATGAGAATCATAATGGTGATGCAGTGAATGCTGGGGAGCCGCCTAAGAGTGTTGATATATGTTCAAATACTGAACCAGCTGAGTGTGTTGATAAAACCATTGTGGAAAGTGGGAAAAGTACTTCTTCAAAGTTGAATGCTGTAAAAGAATCCAAATTTGTAAAGCCCAAGGCCAGCATTTCTCAGGTCCAAAGAAACATTTCTGCTAAGGGATCTGGTAAATATCCTATAGAGAATTCCAGTACTGGACAAAGAGAGCGTACACGAAGAACCGATAAAGAAAAGCTCCCATTAAAGACTGCAATGCCAACTAATAAGGATTCTGTACGCAGAACTCCGAAGTTAGAGGACTCCATGAAGGGAAAATCTTTAAATGAGAGTAAAAGTGTTAAAGAGTCATCGCATAAGAAGCTCGGTGAACTTCAACCTTCTGCATTCAAGCCTGAATCCAGAGGACGCCAAATGACAAACAGGGTTAGTAACGGAGCCAATTTGACTAAGTCTGATACAAGATCAAGTGTTACAACTTTCAAATTCAAAAGTTCTGAGCGAGCAGAAAAGAGGAAAGAGTTCTTTATGAAGTTGGAAGAAAAAATGCACGCTAAGGAAGATGAAATGAACAAGGTCCAAGCAAGAAAACAGGAAAGGACAGAAGCTGAGACGAAGCAATTCCGGAAAGCCCTCAACTTCAAAGCAGCACCAATGCCTTCTTTCTACAATGTAGCTAGCGCTTCGAGGCCTGATGGGAGCAAGGTAAATAAATTCAGTGCATTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

456

Amino Acids

51.14

Weight (kDa)

6.2

Isoelectric Point (pI)

51.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 374 - 439 2.1e-21 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
Acc36I ACCTGC 1 cut(s) 472
AccB1I GGYRCC 1 cut(s) 460
AccIII TCCGGA 1 cut(s) 1263
AciI CCGC 1 cut(s) 632
AclWI GGATC 1 cut(s) 811
AcsI RAATTY 5 cut(s) 235, 746, 826, 1124, 1352
AcyI GRCGYC 1 cut(s) 1048
AfaI GTAC 5 cut(s) 259, 712, 835, 853, 918
AfeI AGCGCT 1 cut(s) 1321
AfiI CCNNNNNNNGG 3 cut(s) 370, 457, 542
AhdI GACNNNNNGTC 1 cut(s) 367
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 8 cut(s) 36, 191, 203, 671, 877, 1003, 1247, 1316
AluI AGCT 8 cut(s) 36, 191, 203, 671, 877, 1003, 1247, 1316
Alw26I GTCTC 1 cut(s) 1244
AlwI GGATC 1 cut(s) 811
AlwNI CAGNNNCTG 1 cut(s) 1247
Ama87I CYCGRG 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 1263
Aor51HI AGCGCT 1 cut(s) 1321
AoxI GGCC 2 cut(s) 762, 1328
ApeKI GCWGC 1 cut(s) 1286
ApoI RAATTY 5 cut(s) 235, 746, 826, 1124, 1352
Asp700I GAANNNNTTC 3 cut(s) 790, 1160, 1259
AspLEI GCGC 2 cut(s) 529, 1322
AspS9I GGNCC 2 cut(s) 778, 1216
AsuHPI GGTGA 2 cut(s) 620, 1019
AsuII TTCGAA 1 cut(s) 84
AsuNHI GCTAGC 1 cut(s) 1316
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 2 cut(s) 778, 1216
BaeGI GKGCMC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 460
BbsI GAAGAC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 1298
BccI CCATC 2 cut(s) 488, 1328
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 1244
BfaI CTAG 1 cut(s) 1317
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 1323
BfuAI ACCTGC 1 cut(s) 472
BisI GCNGC 2 cut(s) 632, 1287
BlsI GCNGC 2 cut(s) 633, 1288
BmcAI AGTACT 2 cut(s) 712, 835
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 778, 1216
BmeRI GACNNNNNGTC 1 cut(s) 367
BmeT110I CYCGRG 1 cut(s) 536
BmgT120I GGNCC 2 cut(s) 778, 1216
BmiI GGNNCC 4 cut(s) 64, 462, 630, 1078
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 3 cut(s) 538, 577, 601
BmtI GCTAGC 1 cut(s) 1320
BpiI GAAGAC 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 599
Bpu10I CCTNAGC 2 cut(s) 798, 1194
Bpu14I TTCGAA 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 1048
BsaJI CCNNGG 1 cut(s) 759
BsaWI WCCGGW 1 cut(s) 1263
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 457, 542
Bse1I ACTGG 3 cut(s) 582, 831, 841
Bse3DI GCAATG 1 cut(s) 900
BseAI TCCGGA 1 cut(s) 1263
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 1 cut(s) 759
BseGI GGATG 1 cut(s) 499
BseLI CCNNNNNNNGG 3 cut(s) 370, 457, 542
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 5 cut(s) 467, 663, 788, 1128, 1239
BseNI ACTGG 3 cut(s) 582, 831, 841
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 1 cut(s) 1298
BseYI CCCAGC 2 cut(s) 5, 623
BshFI GGCC 2 cut(s) 764, 1330
BshNI GGYRCC 1 cut(s) 460
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 1 cut(s) 1264
BslI CCNNNNNNNGG 3 cut(s) 370, 457, 542
BsmAI GTCTC 1 cut(s) 1244
BsmBI CGTCTC 1 cut(s) 1244
BsmI GAATGC 3 cut(s) 625, 733, 1025
BsnI GGCC 2 cut(s) 764, 1330
BsoBI CYCGRG 1 cut(s) 536
Bsp119I TTCGAA 1 cut(s) 84
Bsp1286I GDGCHC 1 cut(s) 463
Bsp13I TCCGGA 1 cut(s) 1263
Bsp143I GATC 2 cut(s) 803, 1103
BspACI CCGC 1 cut(s) 632
BspANI GGCC 2 cut(s) 764, 1330
BspCNI CTCAG 5 cut(s) 466, 664, 787, 1129, 1240
BspEI TCCGGA 1 cut(s) 1263
BspLI GGNNCC 4 cut(s) 64, 462, 630, 1078
BspMI ACCTGC 1 cut(s) 472
BspOI GCTAGC 1 cut(s) 1320
BspPI GGATC 1 cut(s) 811
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 460
BsrDI GCAATG 1 cut(s) 900
BsrI ACTGG 3 cut(s) 582, 831, 841
BssECI CCNNGG 1 cut(s) 759
BssMI GATC 2 cut(s) 803, 1103
BssNI GRCGYC 1 cut(s) 1048
BssT1I CCWWGG 1 cut(s) 759
Bst2UI CCWGG 1 cut(s) 168
Bst6I CTCTTC 1 cut(s) 138
BstACI GRCGYC 1 cut(s) 1048
BstAPI GCANNNNNTGC 1 cut(s) 620
BstBI TTCGAA 1 cut(s) 84
BstC8I GCNNGC 4 cut(s) 766, 1143, 1191, 1318
BstDEI CTNAG 9 cut(s) 453, 636, 672, 774, 798, 1089, 1137, 1194, 1248
BstF5I GGATG 1 cut(s) 499
BstH2I RGCGCY 1 cut(s) 1323
BstHHI GCGC 2 cut(s) 529, 1322
BstKTI GATC 2 cut(s) 806, 1106
BstMAI GTCTC 1 cut(s) 1244
BstMBI GATC 2 cut(s) 803, 1103
BstMWI GCNNNNNNNGC 2 cut(s) 620, 1000
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 819
BstSLI GKGCMC 1 cut(s) 463
BstV1I GCAGC 1 cut(s) 1298
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
BsuRI GGCC 2 cut(s) 764, 1330
BtgZI GCGATG 1 cut(s) 975
BtsCI GGATG 1 cut(s) 499
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 2 cut(s) 621, 1363
BveI ACCTGC 1 cut(s) 472
Cac8I GCNNGC 4 cut(s) 766, 1143, 1191, 1318
CaiI CAGNNNCTG 1 cut(s) 1247
CfoI GCGC 2 cut(s) 529, 1322
Cfr13I GGNCC 2 cut(s) 778, 1216
CseI GACGC 2 cut(s) 311, 1056
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 5 cut(s) 258, 711, 834, 852, 917
CviAII CATG 3 cut(s) 160, 261, 946
CviQI GTAC 5 cut(s) 258, 711, 834, 852, 917
DdeI CTNAG 9 cut(s) 453, 636, 672, 774, 798, 1089, 1137, 1194, 1248
DpnI GATC 2 cut(s) 805, 1105
DpnII GATC 2 cut(s) 803, 1103
DraI TTTAAA 1 cut(s) 963
DriI GACNNNNNGTC 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 138
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 759
Eco147I AGGCCT 1 cut(s) 1330
Eco47I GGWCC 2 cut(s) 778, 1216
Eco47III AGCGCT 1 cut(s) 1321
Eco88I CYCGRG 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 759
EcoT22I ATGCAT 1 cut(s) 161
ErhI CCWWGG 1 cut(s) 759
Esp3I CGTCTC 1 cut(s) 1244
FaeI CATG 3 cut(s) 163, 264, 949
FalI AAGNNNNNCTT 2 cut(s) 700, 732
FatI CATG 3 cut(s) 159, 260, 945
Fnu4HI GCNGC 2 cut(s) 632, 1287
FokI GGATG 1 cut(s) 506
Fsp4HI GCNGC 2 cut(s) 632, 1287
FspBI CTAG 1 cut(s) 1317
GlaI GCGC 2 cut(s) 528, 1321
GluI GCNGC 2 cut(s) 632, 1287
GsaI CCCAGC 2 cut(s) 9, 627
GsuI CTGGAG 1 cut(s) 599
HaeII RGCGCY 1 cut(s) 1323
HaeIII GGCC 2 cut(s) 764, 1330
HapII CCGG 1 cut(s) 1264
HgaI GACGC 2 cut(s) 311, 1056
HhaI GCGC 2 cut(s) 529, 1322
Hin1I GRCGYC 1 cut(s) 1048
Hin1II CATG 3 cut(s) 163, 264, 949
Hin6I GCGC 2 cut(s) 527, 1320
HinP1I GCGC 2 cut(s) 527, 1320
HindIII AAGCTT 1 cut(s) 189
HinfI GANTC 8 cut(s) 353, 472, 598, 740, 911, 941, 986, 1037
HpaII CCGG 1 cut(s) 1264
HphI GGTGA 2 cut(s) 620, 1019
Hpy166II GTNNAC 3 cut(s) 323, 854, 1010
Hpy188I TCNGA 8 cut(s) 292, 358, 373, 448, 456, 930, 1096, 1138
Hpy188III TCNNGA 2 cut(s) 1041, 1264
Hpy8I GTNNAC 3 cut(s) 323, 854, 1010
HpyAV CCTTC 6 cut(s) 86, 211, 329, 943, 1029, 1308
HpyCH4IV ACGT 1 cut(s) 412
HpyCH4V TGCA 9 cut(s) 159, 467, 551, 590, 614, 893, 1025, 1189, 1361
HpyF10VI GCNNNNNNNGC 2 cut(s) 620, 1000
HpyF3I CTNAG 9 cut(s) 453, 636, 672, 774, 798, 1089, 1137, 1194, 1248
HpySE526I ACGT 1 cut(s) 412
Hsp92I GRCGYC 1 cut(s) 1048
Hsp92II CATG 3 cut(s) 163, 264, 949
HspAI GCGC 2 cut(s) 527, 1320
Kpn2I TCCGGA 1 cut(s) 1263
Kzo9I GATC 2 cut(s) 803, 1103
LmnI GCTCC 6 cut(s) 68, 339, 628, 882, 1076, 1338
Lsp1109I GCAGC 1 cut(s) 1298
LweI GCATC 3 cut(s) 538, 577, 601
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 1 cut(s) 1317
MaeII ACGT 1 cut(s) 412
MaeIII GTNAC 3 cut(s) 403, 1070, 1111
MalI GATC 2 cut(s) 805, 1105
MboI GATC 2 cut(s) 803, 1103
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 1 cut(s) 463
MluCI AATT 8 cut(s) 235, 746, 826, 1081, 1124, 1259, 1352, 1366
MlyI GAGTC 2 cut(s) 935, 995
MmeI TCCRAC 2 cut(s) 351, 1155
Mph1103I ATGCAT 1 cut(s) 161
MroI TCCGGA 1 cut(s) 1263
MroXI GAANNNNTTC 3 cut(s) 790, 1160, 1259
MseI TTAA 6 cut(s) 210, 884, 962, 981, 1365, 1369
MslI CAYNNNNRTG 1 cut(s) 606
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 1 cut(s) 1264
MspR9I CCNGG 1 cut(s) 168
Mva1269I GAATGC 3 cut(s) 625, 733, 1025
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 620, 1000
NdeII GATC 2 cut(s) 803, 1103
NheI GCTAGC 1 cut(s) 1316
NlaIII CATG 3 cut(s) 163, 264, 949
NlaIV GGNNCC 4 cut(s) 64, 462, 630, 1078
NmeAIII GCCGAG 1 cut(s) 268
NmuCI GTSAC 1 cut(s) 403
NsiI ATGCAT 1 cut(s) 161
NspI RCATGY 1 cut(s) 264
NspV TTCGAA 1 cut(s) 84
PacI TTAATTAA 1 cut(s) 1369
PaqCI CACCTGC 1 cut(s) 472
PceI AGGCCT 1 cut(s) 1330
PctI GAATGC 3 cut(s) 625, 733, 1025
PdmI GAANNNNTTC 3 cut(s) 790, 1160, 1259
PfeI GAWTC 6 cut(s) 353, 472, 598, 740, 911, 1037
PflFI GACNNNGTC 1 cut(s) 1090
PkrI GCNGC 2 cut(s) 633, 1288
PleI GAGTC 2 cut(s) 935, 994
PpsI GAGTC 2 cut(s) 935, 994
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 2 cut(s) 5, 623
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 4 cut(s) 64, 462, 630, 1078
PspPI GGNCC 2 cut(s) 778, 1216
PstNI CAGNNNCTG 1 cut(s) 1247
PsuI RGATCY 1 cut(s) 803
PsyI GACNNNGTC 1 cut(s) 1090
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 5 cut(s) 259, 712, 835, 853, 918
RsaNI GTAC 5 cut(s) 258, 711, 834, 852, 917
RseI CAYNNNNRTG 1 cut(s) 606
SaqAI TTAA 6 cut(s) 210, 884, 962, 981, 1365, 1369
SatI GCNGC 2 cut(s) 632, 1287
Sau3AI GATC 2 cut(s) 803, 1103
Sau96I GGNCC 2 cut(s) 778, 1216
ScaI AGTACT 2 cut(s) 712, 835
SchI GAGTC 2 cut(s) 935, 995
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 463
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 3 cut(s) 538, 577, 601
SfcI CTRYAG 1 cut(s) 819
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 2 cut(s) 778, 1216
SmiMI CAYNNNNRTG 1 cut(s) 606
Sse9I AATT 8 cut(s) 235, 746, 826, 1081, 1124, 1259, 1352, 1366
SseBI AGGCCT 1 cut(s) 1330
SsiI CCGC 1 cut(s) 632
SspMI CTAG 1 cut(s) 1317
StuI AGGCCT 1 cut(s) 1330
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 1 cut(s) 759
TaiI ACGT 1 cut(s) 415
TaqI TCGA 3 cut(s) 84, 563, 1325
TasI AATT 8 cut(s) 235, 746, 826, 1081, 1124, 1259, 1352, 1366
TatI WGTACW 2 cut(s) 710, 833
TauI GCSGC 1 cut(s) 634
TfiI GAWTC 6 cut(s) 353, 472, 598, 740, 911, 1037
Tru1I TTAA 6 cut(s) 210, 884, 962, 981, 1365, 1369
Tru9I TTAA 6 cut(s) 210, 884, 962, 981, 1365, 1369
TscAI CASTG 2 cut(s) 621, 1363
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 1 cut(s) 1286
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 6 cut(s) 176, 348, 962, 1184, 1218, 1223
TspGWI ACGGA 2 cut(s) 429, 1089
TspRI CASTG 2 cut(s) 621, 1363
Tth111I GACNNNGTC 1 cut(s) 1090
VpaK11BI GGWCC 2 cut(s) 778, 1216
XapI RAATTY 5 cut(s) 235, 746, 826, 1124, 1352
XceI RCATGY 1 cut(s) 264
XmnI GAANNNNTTC 3 cut(s) 790, 1160, 1259
XspI CTAG 1 cut(s) 1317
ZrmI AGTACT 2 cut(s) 712, 835
Zsp2I ATGCAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.