MD16G1028300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0005491
Physical Location & Seq
Forward (+)
1999256 .. 2001955
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1028300.v1.1.491

Sequence Viewer

Length: 678 bp
ATGGCTCATACAATCCAGTTCTTCACAAAGATGGAAGAAAAAATGCATGCCAAGGAAGATGAGATGAATAAGATCCAAGCAAAAAAGCAGGAAAAAGCCGAAGCTGAGATGAGACAACTCCGCAAAAGCCTCAACTTTAAAGCAGCACCAATGCCTTCCTTCTACCATGTAGCTGTGAATGCAGCGCCTGATGGGAGCAAGGTTGTATCTACCAAATTCTCTAAAGTACAAAGCAAGTCAACAAATCCAGGGAGTGGAGCCGCTGCAAGATTTCCATCACATTCAGAGGCAGGCAAGGTTGAACCTCCCACTGCTAACGTTTCTGTAAACACAGCTGATTTGCATGAGGCCTCGAGAGAAACTAACAGCATCATGGCTGATCCCTCTGAAACTAGTTCAGTTTCTTCAGCTACACTGAGCAGCAGAAACTTATCCCGGGATACTGCAACTAAAAGTGAACACCCCAGGACGAAAGGAAGGGAGAAGGATGCTAATTATACGCAGAAACATCGGTTATTAGAGAGCGGTAAAATGGCAAAAAGTCAGAAAGCTGAAGGGAAGCAGAAAGTGGGAGTCCAAAAAAATGGCAGAGAGATGGCAAGGAAGGGTATGAAAGGCGGTGTCGGCTTTGGAAGCAGTTCAAGAATGGGTCATCTAACGGTTGGTGTTGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

226

Amino Acids

24.35

Weight (kDa)

9.99

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 6 - 60 4.8e-17 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 254
AccBSI CCGCTC 1 cut(s) 525
AciI CCGC 4 cut(s) 121, 261, 525, 618
AclI AACGTT 1 cut(s) 318
AclWI GGATC 2 cut(s) 67, 374
AcsI RAATTY 1 cut(s) 215
AcuI CTGAAG 2 cut(s) 390, 573
AfaI GTAC 1 cut(s) 228
AfiI CCNNNNNNNGG 1 cut(s) 254
AgsI TTSAA 2 cut(s) 302, 642
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 2 cut(s) 247, 464
AluBI AGCT 5 cut(s) 104, 173, 335, 410, 551
AluI AGCT 5 cut(s) 104, 173, 335, 410, 551
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 2 cut(s) 67, 374
AlwNI CAGNNNCTG 1 cut(s) 188
Ama87I CYCGRG 2 cut(s) 352, 435
AoxI GGCC 1 cut(s) 348
ApeKI GCWGC 4 cut(s) 143, 182, 263, 420
ApoI RAATTY 1 cut(s) 215
Asp700I GAANNNNTTC 1 cut(s) 637
AspLEI GCGC 1 cut(s) 187
AsuC2I CCSGG 2 cut(s) 436, 437
AvaI CYCGRG 2 cut(s) 352, 435
BbvI GCAGC 4 cut(s) 155, 194, 250, 432
BccI CCATC 4 cut(s) 25, 185, 283, 589
BcgI CGANNNNNNTGC 2 cut(s) 491, 525
BciT130I CCWGG 2 cut(s) 249, 466
BciVI GTATCC 1 cut(s) 433
BcnI CCSGG 2 cut(s) 436, 437
BcoDI GTCTC 1 cut(s) 106
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 1 cut(s) 393
BfoI RGCGCY 1 cut(s) 188
BfuI GTATCC 1 cut(s) 433
BisI GCNGC 5 cut(s) 144, 183, 261, 264, 421
BlsI GCNGC 5 cut(s) 145, 184, 262, 265, 422
Bme1390I CCNGG 4 cut(s) 249, 436, 437, 466
BmeT110I CYCGRG 2 cut(s) 352, 435
BmiI GGNNCC 1 cut(s) 259
BmrFI CCNGG 4 cut(s) 249, 436, 437, 466
BmsI GCATC 2 cut(s) 378, 478
BpuMI CCSGG 2 cut(s) 436, 437
BsaBI GATNNNNATC 1 cut(s) 274
BsaJI CCNNGG 4 cut(s) 51, 248, 435, 464
Bsc4I CCNNNNNNNGG 1 cut(s) 254
Bse1I ACTGG 1 cut(s) 16
Bse8I GATNNNNATC 1 cut(s) 274
BseBI CCWGG 2 cut(s) 249, 466
BseDI CCNNGG 4 cut(s) 51, 248, 435, 464
BseGI GGATG 1 cut(s) 493
BseJI GATNNNNATC 1 cut(s) 274
BseLI CCNNNNNNNGG 1 cut(s) 254
BseMII CTCAG 2 cut(s) 96, 407
BseNI ACTGG 1 cut(s) 16
BseXI GCAGC 4 cut(s) 155, 194, 250, 432
BshFI GGCC 1 cut(s) 350
BsiHKCI CYCGRG 2 cut(s) 352, 435
BsiSI CCGG 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 254
BsmAI GTCTC 1 cut(s) 106
BsmI GAATGC 1 cut(s) 184
BsnI GGCC 1 cut(s) 350
BsoBI CYCGRG 2 cut(s) 352, 435
Bsp143I GATC 2 cut(s) 72, 379
BspACI CCGC 4 cut(s) 121, 261, 525, 618
BspANI GGCC 1 cut(s) 350
BspCNI CTCAG 2 cut(s) 97, 408
BspHI TCATGA 1 cut(s) 674
BspLI GGNNCC 1 cut(s) 259
BspPI GGATC 2 cut(s) 67, 374
BsrBI CCGCTC 1 cut(s) 525
BsrI ACTGG 1 cut(s) 16
BssECI CCNNGG 4 cut(s) 51, 248, 435, 464
BssMI GATC 2 cut(s) 72, 379
BssT1I CCWWGG 1 cut(s) 51
Bst2UI CCWGG 2 cut(s) 249, 466
Bst4CI ACNGT 1 cut(s) 661
BstC8I GCNNGC 2 cut(s) 48, 292
BstDEI CTNAG 2 cut(s) 105, 416
BstF5I GGATG 1 cut(s) 493
BstH2I RGCGCY 1 cut(s) 188
BstHHI GCGC 1 cut(s) 187
BstKTI GATC 2 cut(s) 75, 382
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 2 cut(s) 72, 379
BstMWI GCNNNNNNNGC 3 cut(s) 179, 624, 633
BstNI CCWGG 2 cut(s) 249, 466
BstNSI RCATGY 1 cut(s) 50
BstSCI CCNGG 4 cut(s) 247, 434, 435, 464
BstV1I GCAGC 4 cut(s) 155, 194, 250, 432
BstX2I RGATCY 1 cut(s) 72
BstXI CCANNNNNNTGG 1 cut(s) 584
BstYI RGATCY 1 cut(s) 72
BsuI GTATCC 1 cut(s) 433
BsuRI GGCC 1 cut(s) 350
BtsCI GGATG 1 cut(s) 493
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 2 cut(s) 309, 413
Cac8I GCNNGC 2 cut(s) 48, 292
CaiI CAGNNNCTG 1 cut(s) 188
CciI TCATGA 1 cut(s) 674
CfoI GCGC 1 cut(s) 187
Cfr9I CCCGGG 1 cut(s) 435
Csp6I GTAC 1 cut(s) 227
CviAII CATG 5 cut(s) 47, 167, 344, 373, 675
CviQI GTAC 1 cut(s) 227
DdeI CTNAG 2 cut(s) 105, 416
DpnI GATC 2 cut(s) 74, 381
DpnII GATC 2 cut(s) 72, 379
DraI TTTAAA 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 51
Eco147I AGGCCT 1 cut(s) 350
Eco57I CTGAAG 2 cut(s) 390, 573
Eco88I CYCGRG 2 cut(s) 352, 435
EcoRII CCWGG 2 cut(s) 247, 464
EcoT14I CCWWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 51
FaeI CATG 5 cut(s) 50, 170, 347, 376, 678
FaiI YATR 8 cut(s) 9, 48, 168, 345, 374, 498, 611, 676
FatI CATG 5 cut(s) 46, 166, 343, 372, 674
Fnu4HI GCNGC 5 cut(s) 144, 183, 261, 264, 421
FokI GGATG 1 cut(s) 500
Fsp4HI GCNGC 5 cut(s) 144, 183, 261, 264, 421
FspBI CTAG 1 cut(s) 393
GlaI GCGC 1 cut(s) 186
GluI GCNGC 5 cut(s) 144, 183, 261, 264, 421
HaeII RGCGCY 1 cut(s) 188
HaeIII GGCC 1 cut(s) 350
HapII CCGG 1 cut(s) 436
HhaI GCGC 1 cut(s) 187
Hin1II CATG 5 cut(s) 50, 170, 347, 376, 678
Hin6I GCGC 1 cut(s) 185
HinP1I GCGC 1 cut(s) 185
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HinfI GANTC 1 cut(s) 573
HpaII CCGG 1 cut(s) 436
Hpy166II GTNNAC 3 cut(s) 240, 328, 458
Hpy188I TCNGA 3 cut(s) 286, 388, 546
Hpy188III TCNNGA 3 cut(s) 354, 642, 675
Hpy8I GTNNAC 3 cut(s) 240, 328, 458
HpyAV CCTTC 6 cut(s) 165, 169, 471, 478, 548, 598
HpyCH4III ACNGT 1 cut(s) 661
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 5 cut(s) 46, 182, 266, 343, 446
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 624, 633
HpyF3I CTNAG 2 cut(s) 105, 416
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 5 cut(s) 50, 170, 347, 376, 678
HspAI GCGC 1 cut(s) 185
Kzo9I GATC 2 cut(s) 72, 379
LmnI GCTCC 2 cut(s) 195, 257
LpnPI CCDG 9 cut(s) 29, 74, 201, 234, 261, 276, 449, 451, 478
Lsp1109I GCAGC 4 cut(s) 155, 194, 250, 432
LweI GCATC 2 cut(s) 378, 478
MaeI CTAG 1 cut(s) 393
MaeII ACGT 1 cut(s) 318
MalI GATC 2 cut(s) 74, 381
MbiI CCGCTC 1 cut(s) 525
MboI GATC 2 cut(s) 72, 379
MboII GAAGA 4 cut(s) 13, 47, 68, 396
MflI RGATCY 1 cut(s) 72
MluCI AATT 2 cut(s) 215, 493
MlyI GAGTC 1 cut(s) 582
MnlI CCTC 6 cut(s) 140, 280, 315, 340, 361, 394
Mph1103I ATGCAT 1 cut(s) 48
MroXI GAANNNNTTC 1 cut(s) 637
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 29
MspA1I CMGCKG 2 cut(s) 263, 335
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 4 cut(s) 249, 436, 437, 466
Mva1269I GAATGC 1 cut(s) 184
MvaI CCWGG 2 cut(s) 249, 466
MwoI GCNNNNNNNGC 3 cut(s) 179, 624, 633
NciI CCSGG 2 cut(s) 436, 437
NdeII GATC 2 cut(s) 72, 379
NlaIII CATG 5 cut(s) 50, 170, 347, 376, 678
NlaIV GGNNCC 1 cut(s) 259
NsiI ATGCAT 1 cut(s) 48
NspI RCATGY 1 cut(s) 50
PaeI GCATGC 1 cut(s) 50
PaeR7I CTCGAG 1 cut(s) 352
PagI TCATGA 1 cut(s) 674
PceI AGGCCT 1 cut(s) 350
PctI GAATGC 1 cut(s) 184
PdmI GAANNNNTTC 1 cut(s) 637
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 5 cut(s) 145, 184, 262, 265, 422
PleI GAGTC 1 cut(s) 581
PpsI GAGTC 1 cut(s) 581
Psp1406I AACGTT 1 cut(s) 318
Psp6I CCWGG 2 cut(s) 247, 464
PspGI CCWGG 2 cut(s) 247, 464
PspN4I GGNNCC 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 1 cut(s) 72
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 1 cut(s) 228
RsaNI GTAC 1 cut(s) 227
RseI CAYNNNNRTG 1 cut(s) 29
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 5 cut(s) 144, 183, 261, 264, 421
Sau3AI GATC 2 cut(s) 72, 379
SchI GAGTC 1 cut(s) 582
ScrFI CCNGG 4 cut(s) 249, 436, 437, 466
SetI ASST 9 cut(s) 106, 175, 204, 300, 307, 321, 337, 412, 553
SfaNI GCATC 2 cut(s) 378, 478
Sfr274I CTCGAG 1 cut(s) 352
SlaI CTCGAG 1 cut(s) 352
SmaI CCCGGG 1 cut(s) 437
SmiMI CAYNNNNRTG 1 cut(s) 29
SmlI CTYRAG 1 cut(s) 352
SmoI CTYRAG 1 cut(s) 352
SpeI ACTAGT 1 cut(s) 392
SphI GCATGC 1 cut(s) 50
Sse9I AATT 2 cut(s) 215, 493
SseBI AGGCCT 1 cut(s) 350
SsiI CCGC 4 cut(s) 121, 261, 525, 618
SspMI CTAG 1 cut(s) 393
StuI AGGCCT 1 cut(s) 350
StyD4I CCNGG 4 cut(s) 247, 434, 435, 464
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 1 cut(s) 661
TaiI ACGT 1 cut(s) 321
TaqI TCGA 1 cut(s) 353
TasI AATT 2 cut(s) 215, 493
TatI WGTACW 1 cut(s) 226
TauI GCSGC 1 cut(s) 263
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 2 cut(s) 316, 420
TseI GCWGC 4 cut(s) 143, 182, 263, 420
TspDTI ATGAA 2 cut(s) 80, 626
TspMI CCCGGG 1 cut(s) 435
TspRI CASTG 2 cut(s) 316, 420
Van91I CCANNNNNTGG 1 cut(s) 254
XapI RAATTY 1 cut(s) 215
XceI RCATGY 1 cut(s) 50
XhoI CTCGAG 1 cut(s) 352
XmaI CCCGGG 1 cut(s) 435
XmnI GAANNNNTTC 1 cut(s) 637
XspI CTAG 1 cut(s) 393
Zsp2I ATGCAT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.