Rw4G033130

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005491
Physical Location & Seq
Reverse (-)
60229408 .. 60232935
3528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033130.1

Sequence Viewer

Length: 1707 bp
ATGGCTGGGGAAATAGAAGAACCCTTTTGTTTTAGCTTTCAGCCAAAGTCTATACATTCTGGCTCCATATCATTTGGGAGGTTTGAAAACGAAGGCTTATCTTGGGAAAGGAGGTCTGCTTTCTCACATAACAGATATGTTGAAGAGGTTGAGAAATGCATGAAACCAGGTTCAGTTATTGAGAGGAAAGCTTATTTTGAAGCTCATTTTAAGAAGAAGGGTCTTTCCAGACCAAATTTAGCCGAGTGTTATAGTGGTACATGCTACCAAGTCGGCGAGAATGATGTATCAGAGAGTGATGACGCTTACAGAGAAGAATGTGAACTTGAAAATGAAGGGAGCAGTTATGAAGAATCAGATGACCAAAGGTCGGAGGGTTCTGTGTATCTTGGAGATTTTGAAGTCACAGAATGTGAAAAAGAAGAAGATACTGAACTTCCGTCCTCTGACCCTCAGAGGGCACCTGCATTGAATCATATTGCTATTTTGGTGGATGGTGCTGTTGAAGATGTTAATTTTGAGGAAGCGCATCAACCCGAGAAAGGTTGTGCAAAATCTACTTCGATTGTTGAGGAAATGGAGAGAGATGCAAATGAGAATCATAACGGTGATGCAGTGAATGCTGAGGAGCCGCCTAAGAGTGTTGATATATGTTCAAATACTGAACCAGCTGAGTGTGTTGATAAAACCATTGTGGAAAGTGGGAAAAGTACTTCTTCAAAGTTGAAGGCTGTAAAAGAATCCAAATTTGTAAAGCCTAAGGCCAGCATTTCTCAGGTCCAAAGAAACATTTCTGCTAAGGGATCTAGTAAATATCCTACAGAGAATTCCAATACTGGACAAAGAGAGCGTACACGAAGAACGGGTAAAGAAAAGCTCCCATTAAAGACTGCAATGCCAACTAATAAGCATTCTGTACGTAGAACTCCGAAGTTAGAGGACTCCATGAAGGGAAAATCTTTAAATGAGAGTAAAAGTGTTAAAGAGTCATCGCTTAAGAAGCTTGGTGAACTTCAACCTTCTGCATTCAAGCCTGAATCCAGAGGACGCCAAATGACAAACAGGGTTAGTAACGGAGCCAATTTGACTAAGTCTGATACAAGATCAAGTGTTACAACTTTCAAATTCAAAACTTCTGAGCGAGCAGAAAAGAGGAAAGAGTTCTTTATGAAGTTGGAAGAAAAAATGCACGCTAAGGAAGATGAAATGAACAAGGTCCAAGCAAGAAAACAGTCCGTTACTGGATTAATGCTGTTGCAGGAAAGGATAGAAGCTGAGACGAAGCAATTCCGGAAAGCCCTCAACTTCAAAGCAGCACCAATGCCTTCTTTCTACAATGTAGCTAGCGCTTCGAGGCCTGATGGGAGCATGATTGTCGCAACCAAATCCTCTAAAGTAGAAAAAAGCCGAGACAGTGCTGCTGTAAGATCGACATCAGCTTCAGAGTCAGGGAAGAGTGAAGCTGCCACAGGAGAAACTAAATGCAAGATGGCTGAGGCATTTGTAACAAAAAGTGAAGTCACTACTGTGCAGAGGAGTCGAGCATTCGAGAGCAGGAAGGCGGCGAAAAATGAGAAAAACGTGGGGAAGCAGAAAGTTGGAGCCCAAAGAAATAGCAGAGAGATGGTGAGGAAGGGTACTACGAAAGGCGTGGTCTTTGGAAGCAGTTCCAGAATGAGTCATTTAGCAGTTGGTGTTGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

568

Amino Acids

63.01

Weight (kDa)

8.73

Isoelectric Point (pI)

50.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 374 - 448 5.3e-16 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
Acc36I ACCTGC 1 cut(s) 472
AccB1I GGYRCC 1 cut(s) 460
AccIII TCCGGA 1 cut(s) 1290
AciI CCGC 2 cut(s) 632, 1562
AclWI GGATC 1 cut(s) 811
AcsI RAATTY 4 cut(s) 235, 746, 826, 1124
AcuI CTGAAG 1 cut(s) 1425
AcyI GRCGYC 1 cut(s) 1048
AfaI GTAC 5 cut(s) 259, 712, 853, 918, 1639
AfeI AGCGCT 1 cut(s) 1348
AfiI CCNNNNNNNGG 3 cut(s) 370, 457, 542
AflII CTTAAG 1 cut(s) 995
AhdI GACNNNNNGTC 1 cut(s) 367
AjnI CCWGG 1 cut(s) 166
AleI CACNNNNGTG 1 cut(s) 1526
Alw26I GTCTC 2 cut(s) 1271, 1404
AlwI GGATC 1 cut(s) 811
Ama87I CYCGRG 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 1290
Aor51HI AGCGCT 1 cut(s) 1348
AoxI GGCC 2 cut(s) 762, 1355
ApeKI GCWGC 3 cut(s) 1313, 1418, 1463
ApoI RAATTY 4 cut(s) 235, 746, 826, 1124
AseI ATTAAT 1 cut(s) 1247
Asp700I GAANNNNTTC 4 cut(s) 790, 1160, 1286, 1666
AspLEI GCGC 2 cut(s) 529, 1349
AspS9I GGNCC 2 cut(s) 778, 1216
AsuHPI GGTGA 3 cut(s) 620, 1019, 1639
AsuNHI GCTAGC 1 cut(s) 1343
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 2 cut(s) 778, 1216
AxyI CCTNAGG 1 cut(s) 759
BaeGI GKGCMC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 1606
BbvCI CCTCAGC 2 cut(s) 624, 1494
BbvI GCAGC 3 cut(s) 1325, 1405, 1450
BccI CCATC 4 cut(s) 488, 1355, 1483, 1618
BcgI CGANNNNNNTGC 4 cut(s) 1357, 1391, 1520, 1554
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 1271, 1404
BfaI CTAG 2 cut(s) 807, 1344
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 1350
BfrI CTTAAG 1 cut(s) 995
BfuAI ACCTGC 1 cut(s) 472
BisI GCNGC 5 cut(s) 632, 1314, 1419, 1464, 1563
BlsI GCNGC 5 cut(s) 633, 1315, 1420, 1465, 1564
BmcAI AGTACT 1 cut(s) 712
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 778, 1216
BmeRI GACNNNNNGTC 1 cut(s) 367
BmeT110I CYCGRG 1 cut(s) 536
BmgT120I GGNCC 2 cut(s) 778, 1216
BmiI GGNNCC 5 cut(s) 64, 462, 630, 1078, 1603
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 3 cut(s) 538, 577, 601
BmtI GCTAGC 1 cut(s) 1347
Bpu10I CCTNAGC 4 cut(s) 624, 798, 1194, 1494
BsaAI YACGTR 1 cut(s) 920
BsaBI GATNNNNATC 1 cut(s) 1432
BsaHI GRCGYC 1 cut(s) 1048
BsaWI WCCGGW 1 cut(s) 1290
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 457, 542
Bse1I ACTGG 2 cut(s) 841, 1246
Bse21I CCTNAGG 1 cut(s) 759
Bse3DI GCAATG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 1432
BseAI TCCGGA 1 cut(s) 1290
BseBI CCWGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 499
BseJI GATNNNNATC 1 cut(s) 1432
BseLI CCNNNNNNNGG 3 cut(s) 370, 457, 542
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 7 cut(s) 467, 615, 663, 788, 1128, 1266, 1485
BseNI ACTGG 2 cut(s) 841, 1246
BseRI GAGGAG 2 cut(s) 641, 1549
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 3 cut(s) 1325, 1405, 1450
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 1550
BshFI GGCC 2 cut(s) 764, 1357
BshNI GGYRCC 1 cut(s) 460
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 1 cut(s) 1291
BslI CCNNNNNNNGG 3 cut(s) 370, 457, 542
BsmAI GTCTC 2 cut(s) 1271, 1404
BsmBI CGTCTC 1 cut(s) 1271
BsmI GAATGC 4 cut(s) 625, 910, 1025, 1544
BsnI GGCC 2 cut(s) 764, 1357
BsoBI CYCGRG 1 cut(s) 536
Bsp1286I GDGCHC 2 cut(s) 463, 1606
Bsp13I TCCGGA 1 cut(s) 1290
Bsp143I GATC 3 cut(s) 803, 1103, 1427
BspACI CCGC 2 cut(s) 632, 1562
BspANI GGCC 2 cut(s) 764, 1357
BspCNI CTCAG 7 cut(s) 466, 616, 664, 787, 1129, 1267, 1486
BspEI TCCGGA 1 cut(s) 1290
BspHI TCATGA 1 cut(s) 1703
BspLI GGNNCC 5 cut(s) 64, 462, 630, 1078, 1603
BspMI ACCTGC 1 cut(s) 472
BspOI GCTAGC 1 cut(s) 1347
BspPI GGATC 1 cut(s) 811
BspT107I GGYRCC 1 cut(s) 460
BspTI CTTAAG 1 cut(s) 995
BsrDI GCAATG 1 cut(s) 900
BsrI ACTGG 2 cut(s) 841, 1246
BssMI GATC 3 cut(s) 803, 1103, 1427
BssNI GRCGYC 1 cut(s) 1048
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 4 cut(s) 608, 1233, 1415, 1528
Bst6I CTCTTC 2 cut(s) 138, 1448
BstACI GRCGYC 1 cut(s) 1048
BstAFI CTTAAG 1 cut(s) 995
BstAPI GCANNNNNTGC 1 cut(s) 620
BstBAI YACGTR 1 cut(s) 920
BstC8I GCNNGC 4 cut(s) 766, 1143, 1191, 1345
BstF5I GGATG 1 cut(s) 499
BstH2I RGCGCY 1 cut(s) 1350
BstHHI GCGC 2 cut(s) 529, 1349
BstKTI GATC 3 cut(s) 806, 1106, 1430
BstMAI GTCTC 2 cut(s) 1271, 1404
BstMBI GATC 3 cut(s) 803, 1103, 1427
BstMWI GCNNNNNNNGC 2 cut(s) 620, 1000
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 819
BstSLI GKGCMC 1 cut(s) 463
BstSNI TACGTA 1 cut(s) 920
BstV1I GCAGC 3 cut(s) 1325, 1405, 1450
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
Bsu36I CCTNAGG 1 cut(s) 759
BsuRI GGCC 2 cut(s) 764, 1357
BtgZI GCGATG 1 cut(s) 975
BtsCI GGATG 1 cut(s) 499
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 2 cut(s) 621, 1420
BveI ACCTGC 1 cut(s) 472
Cac8I GCNNGC 4 cut(s) 766, 1143, 1191, 1345
CciI TCATGA 1 cut(s) 1703
CfoI GCGC 2 cut(s) 529, 1349
Cfr13I GGNCC 2 cut(s) 778, 1216
CseI GACGC 2 cut(s) 311, 1056
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 5 cut(s) 258, 711, 852, 917, 1638
CviAII CATG 5 cut(s) 160, 261, 946, 1369, 1704
CviQI GTAC 5 cut(s) 258, 711, 852, 917, 1638
DpnI GATC 3 cut(s) 805, 1105, 1429
DpnII GATC 3 cut(s) 803, 1103, 1427
DraI TTTAAA 1 cut(s) 963
DriI GACNNNNNGTC 1 cut(s) 367
Eam1104I CTCTTC 2 cut(s) 138, 1448
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 2 cut(s) 138, 1448
Eco105I TACGTA 1 cut(s) 920
Eco147I AGGCCT 1 cut(s) 1357
Eco24I GRGCYC 1 cut(s) 1606
Eco47I GGWCC 2 cut(s) 778, 1216
Eco47III AGCGCT 1 cut(s) 1348
Eco57I CTGAAG 1 cut(s) 1425
Eco81I CCTNAGG 1 cut(s) 759
Eco88I CYCGRG 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 1 cut(s) 166
EcoT22I ATGCAT 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 1606
Esp3I CGTCTC 1 cut(s) 1271
FaeI CATG 5 cut(s) 163, 264, 949, 1372, 1707
FalI AAGNNNNNCTT 2 cut(s) 700, 732
FatI CATG 5 cut(s) 159, 260, 945, 1368, 1703
Fnu4HI GCNGC 5 cut(s) 632, 1314, 1419, 1464, 1563
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 1 cut(s) 1606
Fsp4HI GCNGC 5 cut(s) 632, 1314, 1419, 1464, 1563
FspBI CTAG 2 cut(s) 807, 1344
GlaI GCGC 2 cut(s) 528, 1348
GluI GCNGC 5 cut(s) 632, 1314, 1419, 1464, 1563
GsaI CCCAGC 1 cut(s) 9
HaeII RGCGCY 1 cut(s) 1350
HaeIII GGCC 2 cut(s) 764, 1357
HapII CCGG 1 cut(s) 1291
HgaI GACGC 2 cut(s) 311, 1056
HhaI GCGC 2 cut(s) 529, 1349
Hin1I GRCGYC 1 cut(s) 1048
Hin1II CATG 5 cut(s) 163, 264, 949, 1372, 1707
Hin6I GCGC 2 cut(s) 527, 1347
HinP1I GCGC 2 cut(s) 527, 1347
HindIII AAGCTT 2 cut(s) 189, 1001
HpaII CCGG 1 cut(s) 1291
HphI GGTGA 3 cut(s) 620, 1019, 1639
Hpy166II GTNNAC 3 cut(s) 323, 854, 1010
Hpy188I TCNGA 9 cut(s) 292, 358, 373, 448, 456, 930, 1096, 1138, 1444
Hpy188III TCNNGA 6 cut(s) 228, 1041, 1291, 1549, 1671, 1704
Hpy8I GTNNAC 3 cut(s) 323, 854, 1010
HpyAV CCTTC 9 cut(s) 86, 211, 329, 721, 943, 1029, 1335, 1552, 1627
HpyCH4III ACNGT 4 cut(s) 608, 1233, 1415, 1528
HpyCH4IV ACGT 2 cut(s) 919, 1581
HpyF10VI GCNNNNNNNGC 2 cut(s) 620, 1000
HpySE526I ACGT 2 cut(s) 919, 1581
Hsp92I GRCGYC 1 cut(s) 1048
Hsp92II CATG 5 cut(s) 163, 264, 949, 1372, 1707
HspAI GCGC 2 cut(s) 527, 1347
Kpn2I TCCGGA 1 cut(s) 1290
Kzo9I GATC 3 cut(s) 803, 1103, 1427
LmnI GCTCC 7 cut(s) 68, 339, 628, 882, 1076, 1365, 1601
Lsp1109I GCAGC 3 cut(s) 1325, 1405, 1450
LweI GCATC 3 cut(s) 538, 577, 601
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 2 cut(s) 807, 1344
MaeII ACGT 2 cut(s) 919, 1581
MaeIII GTNAC 6 cut(s) 403, 1070, 1111, 1237, 1504, 1519
MalI GATC 3 cut(s) 805, 1105, 1429
MboI GATC 3 cut(s) 803, 1103, 1427
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 2 cut(s) 463, 1606
MluCI AATT 7 cut(s) 235, 514, 746, 826, 1081, 1124, 1286
MlyI GAGTC 5 cut(s) 935, 995, 1454, 1546, 1687
MmeI TCCRAC 3 cut(s) 351, 1155, 1579
Mph1103I ATGCAT 1 cut(s) 161
MroI TCCGGA 1 cut(s) 1290
MroXI GAANNNNTTC 4 cut(s) 790, 1160, 1286, 1666
MseI TTAA 7 cut(s) 210, 513, 884, 962, 981, 996, 1247
MslI CAYNNNNRTG 2 cut(s) 606, 1526
MspA1I CMGCKG 1 cut(s) 671
MspCI CTTAAG 1 cut(s) 995
MspI CCGG 1 cut(s) 1291
MspR9I CCNGG 1 cut(s) 168
Mva1269I GAATGC 4 cut(s) 625, 910, 1025, 1544
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 620, 1000
NdeII GATC 3 cut(s) 803, 1103, 1427
NheI GCTAGC 1 cut(s) 1343
NlaIII CATG 5 cut(s) 163, 264, 949, 1372, 1707
NlaIV GGNNCC 5 cut(s) 64, 462, 630, 1078, 1603
NmeAIII GCCGAG 2 cut(s) 268, 1433
NmuCI GTSAC 2 cut(s) 403, 1519
NsiI ATGCAT 1 cut(s) 161
NspI RCATGY 1 cut(s) 264
OliI CACNNNNGTG 1 cut(s) 1526
PagI TCATGA 1 cut(s) 1703
PaqCI CACCTGC 1 cut(s) 472
PceI AGGCCT 1 cut(s) 1357
PctI GAATGC 4 cut(s) 625, 910, 1025, 1544
PdmI GAANNNNTTC 4 cut(s) 790, 1160, 1286, 1666
PfeI GAWTC 5 cut(s) 353, 472, 598, 740, 1037
PflFI GACNNNGTC 1 cut(s) 1090
PkrI GCNGC 5 cut(s) 633, 1315, 1420, 1465, 1564
PleI GAGTC 5 cut(s) 935, 994, 1453, 1545, 1686
PpsI GAGTC 5 cut(s) 935, 994, 1453, 1545, 1686
Ppu21I YACGTR 1 cut(s) 920
PshBI ATTAAT 1 cut(s) 1247
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 5 cut(s) 64, 462, 630, 1078, 1603
PspPI GGNCC 2 cut(s) 778, 1216
PsuI RGATCY 1 cut(s) 803
PsyI GACNNNGTC 1 cut(s) 1090
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 5 cut(s) 259, 712, 853, 918, 1639
RsaNI GTAC 5 cut(s) 258, 711, 852, 917, 1638
RseI CAYNNNNRTG 2 cut(s) 606, 1526
SaqAI TTAA 7 cut(s) 210, 513, 884, 962, 981, 996, 1247
SatI GCNGC 5 cut(s) 632, 1314, 1419, 1464, 1563
Sau3AI GATC 3 cut(s) 803, 1103, 1427
Sau96I GGNCC 2 cut(s) 778, 1216
ScaI AGTACT 1 cut(s) 712
SchI GAGTC 5 cut(s) 935, 995, 1454, 1546, 1687
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 2 cut(s) 463, 1606
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 3 cut(s) 538, 577, 601
SfcI CTRYAG 1 cut(s) 819
SinI GGWCC 2 cut(s) 778, 1216
SmiMI CAYNNNNRTG 2 cut(s) 606, 1526
SmlI CTYRAG 1 cut(s) 995
SmoI CTYRAG 1 cut(s) 995
SnaBI TACGTA 1 cut(s) 920
Sse9I AATT 7 cut(s) 235, 514, 746, 826, 1081, 1124, 1286
SseBI AGGCCT 1 cut(s) 1357
SsiI CCGC 2 cut(s) 632, 1562
SspMI CTAG 2 cut(s) 807, 1344
StuI AGGCCT 1 cut(s) 1357
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 4 cut(s) 608, 1233, 1415, 1528
TaiI ACGT 2 cut(s) 922, 1584
TaqI TCGA 5 cut(s) 563, 1352, 1430, 1540, 1548
TasI AATT 7 cut(s) 235, 514, 746, 826, 1081, 1124, 1286
TatI WGTACW 1 cut(s) 710
TauI GCSGC 2 cut(s) 634, 1565
TfiI GAWTC 5 cut(s) 353, 472, 598, 740, 1037
Tru1I TTAA 7 cut(s) 210, 513, 884, 962, 981, 996, 1247
Tru9I TTAA 7 cut(s) 210, 513, 884, 962, 981, 996, 1247
TscAI CASTG 2 cut(s) 621, 1420
TseFI GTSAC 2 cut(s) 403, 1519
TseI GCWGC 3 cut(s) 1313, 1418, 1463
Tsp45I GTSAC 2 cut(s) 403, 1519
TspDTI ATGAA 7 cut(s) 176, 348, 363, 962, 1184, 1218, 1223
TspGWI ACGGA 3 cut(s) 429, 1089, 1225
TspRI CASTG 2 cut(s) 621, 1420
Tth111I GACNNNGTC 1 cut(s) 1090
Vha464I CTTAAG 1 cut(s) 995
VpaK11BI GGWCC 2 cut(s) 778, 1216
VspI ATTAAT 1 cut(s) 1247
XapI RAATTY 4 cut(s) 235, 746, 826, 1124
XceI RCATGY 1 cut(s) 264
XmnI GAANNNNTTC 4 cut(s) 790, 1160, 1286, 1666
XspI CTAG 2 cut(s) 807, 1344
ZrmI AGTACT 1 cut(s) 712
Zsp2I ATGCAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.