FvH4_4g32430

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005491
Physical Location & Seq
Reverse (-)
31070400 .. 31074261
3862 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32430.t1

Sequence Viewer

Length: 1683 bp
ATGGCTGGGGAAATAGAAGAACCCTTTAGTTTTAGCTTTCAGGCAGATTCTATACATTCCGGCTCGATATCATTTGGGAGGTTCGAAAATGAAGGCTTGTCTTGGGAAAGGAGGTCTGCTTTCTCACATAACAGATATGTTGAAGAAGTTGAGAAATGCATGAAACCAGGTTCAGTTATTGAAAGGAAAGCTTATTTTGAAGCTCATTTTAAGAAGAAGGGTTTTCCCAGACCAAATTTAGCCGAGTGTTACAGTGGCACGGACTATAGAGTCTGCGGAAATGATGTATCTGAGTGTGATGATGCTTACAGAGAAGAATGTGAACTTGAAAATGAAGAGAATAGTTATGCTGAATCTGATGAGAGGTCAGAGGATTCTGTCACATCTGTGTATCTTGGAGACTTTGAAGTCACAGAATGTGAAAAAGAAGATCCTGAACTTTCATCATCTGACACTCAGAGGGCGCCTGCATTGAATCATATTGCTATTTTGGTGGATGGTGCAGTTGAAGATGTTAATTTTGAGGAACTGCATCAACCCGAGGAAGGTTGTGAAAACTCATCGATTGTTGAGGAACTGGAGAGAAATGTAAACGAGAATCGCAATGGTGATGCAGAGAATTCTGAGGAGCCATCTAAGAAGTCTGTTAATATATGTTCAAAGACTGAACCAGCTGAGTGTGTTGACAAAACCATTGTGGAAAGTGGCAAAAGTACTTCTTCAAAGTTGAAGGCTGTACAAGAGTCCAAATTTGTAAAGCCTAAGGCCAGCATATCTCAGGTCCAGAGAGGCAATTGCGCTAAGGCATCTGGTAAACATCCTATACAGAATTTCAATACTAGACAAAGAGAGCGTACACGAAACATGATTAAAGAAAAGCTCCCATCAAAAACTGCAACGCCAACTACGCAATCTGTATGCAGGACTCCAAAGTTAGAGGACTCCATGAAGGGAAAATCTTCCATTATTGAGAGTAAAAGTGATAGAGTGTCATCGAGTAAGAAGCTTGGTGAACCTCAACCTTCTGCATTCAACCCTGAATCCAGAGGACGCCAGATGACAAACAGGATTAGTAACAGAGCCAATGTGACCAAGTCTGATGGATCAACTGCTACAACTTTCAAATTCAAAAGTTCTGAGCGAGCAGAAAAGAGGAAAGAGTTCGTTATGAGGTTGGAAGAAAAAATGCACGCTAAGGAAGATGAAATGAACAAGGTCCAAGCAAGAAAACAGGAAAAGACAGAAGCTGAGATGAAGCAATTCCGGAAAGCCCTCAACTTCAAAGCATCACCAATGCCTTCTTTCTACAACGCAGCTACAGCTTCGAGGCCAGATGGGAGCAAGATTGCCTCAGCCAAGTCCTCTAAAGTAGAAAAAAGCCGAGGTAACGCTAATGCTGTAAGATTGACATCACCTTCAGAGTCAGGAAAGAGTGAAGCTGCCACAGGAGAAACTAAATGCACCATGGATGAGGCCTTTGTTACAAAAACTGAAGTCACTACTGTGCGGAGGAGTCAAGTATTTGAAAGCAGGAAGGTGGTGAAAAATGAGAAACATGTGGGGAAGCCGAAAGTGGGAGCCGAAAGAAATAGCAGAGAGATGTTAAGGAAGGGTACTATGAAAGGTGTTGGCTTTGGAAGCAGTTCCAGAATGAGTCATCTAGCAGTTGGTGTTGCTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

561

Amino Acids

62.37

Weight (kDa)

8.11

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 374 - 440 9.6e-21 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 269, 407
AccB1I GGYRCC 1 cut(s) 463
AccIII TCCGGA 1 cut(s) 1263
AciI CCGC 2 cut(s) 276, 1507
AclWI GGATC 2 cut(s) 425, 1111
AcsI RAATTY 5 cut(s) 235, 619, 749, 829, 1124
AcuI CTGAAG 2 cut(s) 1401, 1512
AcyI GRCGYC 2 cut(s) 464, 1051
AfaI GTAC 4 cut(s) 715, 738, 856, 1615
AfiI CCNNNNNNNGG 2 cut(s) 545, 1574
AflIII ACRYGT 1 cut(s) 1555
AjnI CCWGG 1 cut(s) 166
AleI CACNNNNGTG 2 cut(s) 386, 1502
Alw26I GTCTC 1 cut(s) 393
AlwI GGATC 2 cut(s) 425, 1111
AlwNI CAGNNNCTG 1 cut(s) 1247
Ama87I CYCGRG 1 cut(s) 539
Aor13HI TCCGGA 1 cut(s) 1263
AoxI GGCC 3 cut(s) 765, 1328, 1473
ApeKI GCWGC 2 cut(s) 1313, 1439
ApoI RAATTY 5 cut(s) 235, 619, 749, 829, 1124
Asp700I GAANNNNTTC 4 cut(s) 958, 1160, 1259, 1642
AspLEI GCGC 2 cut(s) 466, 800
AspS9I GGNCC 2 cut(s) 781, 1216
AsuHPI GGTGA 5 cut(s) 620, 1022, 1281, 1404, 1552
AsuII TTCGAA 1 cut(s) 84
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 2 cut(s) 781, 1216
AxyI CCTNAGG 1 cut(s) 762
BanI GGYRCC 1 cut(s) 463
BbvCI CCTCAGC 1 cut(s) 1351
BbvI GCAGC 2 cut(s) 1325, 1426
BccI CCATC 5 cut(s) 491, 640, 892, 1094, 1328
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 393
BfaI CTAG 2 cut(s) 840, 1661
BfmI CTRYAG 2 cut(s) 265, 1317
BfoI RGCGCY 1 cut(s) 467
BisI GCNGC 2 cut(s) 1314, 1440
BlsI GCNGC 2 cut(s) 1315, 1441
BmcAI AGTACT 1 cut(s) 715
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 781, 1216
BmeT110I CYCGRG 1 cut(s) 539
BmgT120I GGNCC 2 cut(s) 781, 1216
BmiI GGNNCC 3 cut(s) 465, 630, 1579
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 5 cut(s) 292, 541, 601, 815, 1295
BpmI CTGGAG 1 cut(s) 599
Bpu10I CCTNAGC 3 cut(s) 801, 1194, 1351
Bpu14I TTCGAA 1 cut(s) 84
Bsa29I ATCGAT 1 cut(s) 563
BsaBI GATNNNNATC 1 cut(s) 1408
BsaHI GRCGYC 2 cut(s) 464, 1051
BsaJI CCNNGG 3 cut(s) 540, 1381, 1464
BsaWI WCCGGW 1 cut(s) 1263
Bsc4I CCNNNNNNNGG 2 cut(s) 545, 1574
Bse1I ACTGG 1 cut(s) 582
Bse21I CCTNAGG 1 cut(s) 762
Bse3DI GCAATG 1 cut(s) 610
Bse8I GATNNNNATC 1 cut(s) 1408
BseAI TCCGGA 1 cut(s) 1263
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 563
BseDI CCNNGG 3 cut(s) 540, 1381, 1464
BseGI GGATG 3 cut(s) 502, 817, 1474
BseJI GATNNNNATC 1 cut(s) 1408
BseLI CCNNNNNNNGG 2 cut(s) 545, 1574
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 8 cut(s) 282, 470, 615, 666, 791, 1128, 1239, 1365
BseNI ACTGG 1 cut(s) 582
BseRI GAGGAG 2 cut(s) 641, 1525
BseXI GCAGC 2 cut(s) 1325, 1426
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 3 cut(s) 767, 1330, 1475
BshNI GGYRCC 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 563
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 2 cut(s) 60, 1264
BslI CCNNNNNNNGG 2 cut(s) 545, 1574
BsmAI GTCTC 1 cut(s) 393
BsmI GAATGC 1 cut(s) 1028
BsnI GGCC 3 cut(s) 767, 1330, 1475
BsoBI CYCGRG 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 84
Bsp13I TCCGGA 1 cut(s) 1263
Bsp1407I TGTACA 1 cut(s) 736
Bsp143I GATC 2 cut(s) 430, 1103
Bsp19I CCATGG 1 cut(s) 1464
BspACI CCGC 2 cut(s) 276, 1507
BspANI GGCC 3 cut(s) 767, 1330, 1475
BspCNI CTCAG 8 cut(s) 283, 469, 616, 667, 790, 1129, 1240, 1364
BspDI ATCGAT 1 cut(s) 563
BspEI TCCGGA 1 cut(s) 1263
BspLI GGNNCC 3 cut(s) 465, 630, 1579
BspPI GGATC 2 cut(s) 425, 1111
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 463
BsrDI GCAATG 1 cut(s) 610
BsrGI TGTACA 1 cut(s) 736
BsrI ACTGG 1 cut(s) 582
BssECI CCNNGG 3 cut(s) 540, 1381, 1464
BssMI GATC 2 cut(s) 430, 1103
BssNI GRCGYC 2 cut(s) 464, 1051
BssT1I CCWWGG 1 cut(s) 1464
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 254, 1504
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 2 cut(s) 464, 1051
BstAUI TGTACA 1 cut(s) 736
BstBI TTCGAA 1 cut(s) 84
BstC8I GCNNGC 4 cut(s) 468, 769, 1143, 1191
BstDSI CCRYGG 1 cut(s) 1464
BstF5I GGATG 3 cut(s) 502, 817, 1474
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 2 cut(s) 466, 800
BstKTI GATC 2 cut(s) 433, 1106
BstMAI GTCTC 1 cut(s) 393
BstMBI GATC 2 cut(s) 430, 1103
BstMWI GCNNNNNNNGC 3 cut(s) 907, 1319, 1638
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 1559
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 2 cut(s) 265, 1317
BstV1I GCAGC 2 cut(s) 1325, 1426
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
Bsu15I ATCGAT 1 cut(s) 563
Bsu36I CCTNAGG 1 cut(s) 762
BsuRI GGCC 3 cut(s) 767, 1330, 1475
BsuTUI ATCGAT 1 cut(s) 563
BtgI CCRYGG 1 cut(s) 1464
BtsCI GGATG 3 cut(s) 502, 817, 1474
BtsIMutI CAGTG 1 cut(s) 259
Cac8I GCNNGC 4 cut(s) 468, 769, 1143, 1191
CaiI CAGNNNCTG 1 cut(s) 1247
CfoI GCGC 2 cut(s) 466, 800
Cfr13I GGNCC 2 cut(s) 781, 1216
ClaI ATCGAT 1 cut(s) 563
CseI GACGC 1 cut(s) 1059
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 4 cut(s) 714, 737, 855, 1614
CviAII CATG 5 cut(s) 160, 865, 946, 1465, 1556
CviQI GTAC 4 cut(s) 714, 737, 855, 1614
DinI GGCGCC 1 cut(s) 465
DpnI GATC 2 cut(s) 432, 1105
DpnII GATC 2 cut(s) 430, 1103
DrdI GACNNNNNNGTC 2 cut(s) 269, 407
DseDI GACNNNNNNGTC 2 cut(s) 269, 407
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 1464
Eco147I AGGCCT 1 cut(s) 1475
Eco32I GATATC 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 781, 1216
Eco57I CTGAAG 2 cut(s) 1401, 1512
Eco81I CCTNAGG 1 cut(s) 762
Eco88I CYCGRG 1 cut(s) 539
EcoRI GAATTC 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 166
EcoRV GATATC 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 1464
EcoT22I ATGCAT 1 cut(s) 161
EgeI GGCGCC 1 cut(s) 465
EheI GGCGCC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 1464
FaeI CATG 5 cut(s) 163, 868, 949, 1468, 1559
FalI AAGNNNNNCTT 4 cut(s) 175, 207, 703, 735
FatI CATG 5 cut(s) 159, 864, 945, 1464, 1555
Fnu4HI GCNGC 2 cut(s) 1314, 1440
FokI GGATG 3 cut(s) 509, 804, 1481
Fsp4HI GCNGC 2 cut(s) 1314, 1440
FspBI CTAG 2 cut(s) 840, 1661
GlaI GCGC 2 cut(s) 465, 799
GluI GCNGC 2 cut(s) 1314, 1440
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 599
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 3 cut(s) 767, 1330, 1475
HapII CCGG 2 cut(s) 60, 1264
HgaI GACGC 1 cut(s) 1059
HhaI GCGC 2 cut(s) 466, 800
Hin1I GRCGYC 2 cut(s) 464, 1051
Hin1II CATG 5 cut(s) 163, 868, 949, 1468, 1559
Hin6I GCGC 2 cut(s) 464, 798
HinP1I GCGC 2 cut(s) 464, 798
HincII GTYRAC 1 cut(s) 685
HindII GTYRAC 1 cut(s) 685
HindIII AAGCTT 2 cut(s) 189, 1004
HpaII CCGG 2 cut(s) 60, 1264
HphI GGTGA 5 cut(s) 620, 1022, 1281, 1404, 1552
Hpy166II GTNNAC 6 cut(s) 323, 592, 685, 815, 857, 1013
Hpy188I TCNGA 9 cut(s) 292, 358, 370, 451, 459, 625, 1099, 1138, 1420
Hpy188III TCNNGA 7 cut(s) 434, 784, 1044, 1264, 1425, 1647, 1680
Hpy8I GTNNAC 6 cut(s) 323, 592, 685, 815, 857, 1013
HpyCH4III ACNGT 2 cut(s) 254, 1504
HpyF10VI GCNNNNNNNGC 3 cut(s) 907, 1319, 1638
Hsp92I GRCGYC 2 cut(s) 464, 1051
Hsp92II CATG 5 cut(s) 163, 868, 949, 1468, 1559
HspAI GCGC 2 cut(s) 464, 798
KasI GGCGCC 1 cut(s) 463
Kpn2I TCCGGA 1 cut(s) 1263
Kzo9I GATC 2 cut(s) 430, 1103
LmnI GCTCC 4 cut(s) 628, 885, 1338, 1577
Lsp1109I GCAGC 2 cut(s) 1325, 1426
LweI GCATC 5 cut(s) 292, 541, 601, 815, 1295
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 2 cut(s) 840, 1661
MaeIII GTNAC 8 cut(s) 248, 379, 409, 1073, 1087, 1385, 1480, 1495
MalI GATC 2 cut(s) 432, 1105
MboI GATC 2 cut(s) 430, 1103
MfeI CAATTG 1 cut(s) 793
MflI RGATCY 1 cut(s) 430
MluCI AATT 8 cut(s) 235, 517, 619, 749, 793, 829, 1124, 1259
Mly113I GGCGCC 1 cut(s) 464
MlyI GAGTC 7 cut(s) 279, 752, 919, 935, 1430, 1522, 1663
MmeI TCCRAC 1 cut(s) 1155
Mph1103I ATGCAT 1 cut(s) 161
MroI TCCGGA 1 cut(s) 1263
MroXI GAANNNNTTC 4 cut(s) 958, 1160, 1259, 1642
MseI TTAA 5 cut(s) 210, 516, 648, 870, 1604
MslI CAYNNNNRTG 2 cut(s) 386, 1502
MspA1I CMGCKG 1 cut(s) 674
MspI CCGG 2 cut(s) 60, 1264
MspR9I CCNGG 1 cut(s) 168
MunI CAATTG 1 cut(s) 793
Mva1269I GAATGC 1 cut(s) 1028
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 3 cut(s) 907, 1319, 1638
NarI GGCGCC 1 cut(s) 464
NcoI CCATGG 1 cut(s) 1464
NdeII GATC 2 cut(s) 430, 1103
NlaIII CATG 5 cut(s) 163, 868, 949, 1468, 1559
NlaIV GGNNCC 3 cut(s) 465, 630, 1579
NmeAIII GCCGAG 2 cut(s) 268, 1406
NmuCI GTSAC 4 cut(s) 379, 409, 1087, 1495
NsiI ATGCAT 1 cut(s) 161
NspI RCATGY 1 cut(s) 1559
NspV TTCGAA 1 cut(s) 84
OliI CACNNNNGTG 2 cut(s) 386, 1502
PceI AGGCCT 1 cut(s) 1475
PciI ACATGT 1 cut(s) 1555
PctI GAATGC 1 cut(s) 1028
PdmI GAANNNNTTC 4 cut(s) 958, 1160, 1259, 1642
PfeI GAWTC 6 cut(s) 47, 353, 374, 475, 598, 1040
PflFI GACNNNGTC 1 cut(s) 1093
PkrI GCNGC 2 cut(s) 1315, 1441
PleI GAGTC 7 cut(s) 278, 751, 919, 935, 1429, 1521, 1662
PluTI GGCGCC 1 cut(s) 467
PpsI GAGTC 7 cut(s) 278, 751, 919, 935, 1429, 1521, 1662
PscI ACATGT 1 cut(s) 1555
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 3 cut(s) 465, 630, 1579
PspPI GGNCC 2 cut(s) 781, 1216
PstNI CAGNNNCTG 1 cut(s) 1247
PsuI RGATCY 1 cut(s) 430
PsyI GACNNNGTC 1 cut(s) 1093
PvuII CAGCTG 1 cut(s) 674
RsaI GTAC 4 cut(s) 715, 738, 856, 1615
RsaNI GTAC 4 cut(s) 714, 737, 855, 1614
RseI CAYNNNNRTG 2 cut(s) 386, 1502
SaqAI TTAA 5 cut(s) 210, 516, 648, 870, 1604
SatI GCNGC 2 cut(s) 1314, 1440
Sau3AI GATC 2 cut(s) 430, 1103
Sau96I GGNCC 2 cut(s) 781, 1216
ScaI AGTACT 1 cut(s) 715
SchI GAGTC 7 cut(s) 279, 752, 919, 935, 1430, 1522, 1663
ScrFI CCNGG 1 cut(s) 168
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 5 cut(s) 292, 541, 601, 815, 1295
SfcI CTRYAG 2 cut(s) 265, 1317
SfoI GGCGCC 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 2 cut(s) 781, 1216
SmiMI CAYNNNNRTG 2 cut(s) 386, 1502
Sse9I AATT 8 cut(s) 235, 517, 619, 749, 793, 829, 1124, 1259
SseBI AGGCCT 1 cut(s) 1475
SsiI CCGC 2 cut(s) 276, 1507
SspDI GGCGCC 1 cut(s) 463
SspMI CTAG 2 cut(s) 840, 1661
StuI AGGCCT 1 cut(s) 1475
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 1 cut(s) 1464
TaaI ACNGT 2 cut(s) 254, 1504
TaqI TCGA 5 cut(s) 65, 84, 563, 995, 1325
TasI AATT 8 cut(s) 235, 517, 619, 749, 793, 829, 1124, 1259
TatI WGTACW 2 cut(s) 713, 736
TfiI GAWTC 6 cut(s) 47, 353, 374, 475, 598, 1040
Tru1I TTAA 5 cut(s) 210, 516, 648, 870, 1604
Tru9I TTAA 5 cut(s) 210, 516, 648, 870, 1604
TscAI CASTG 1 cut(s) 259
TseFI GTSAC 4 cut(s) 379, 409, 1087, 1495
TseI GCWGC 2 cut(s) 1313, 1439
Tsp45I GTSAC 4 cut(s) 379, 409, 1087, 1495
TspDTI ATGAA 9 cut(s) 105, 176, 348, 432, 962, 1218, 1223, 1268, 1634
TspGWI ACGGA 1 cut(s) 275
TspRI CASTG 1 cut(s) 259
Tth111I GACNNNGTC 1 cut(s) 1093
VpaK11BI GGWCC 2 cut(s) 781, 1216
XapI RAATTY 5 cut(s) 235, 619, 749, 829, 1124
XceI RCATGY 1 cut(s) 1559
XmnI GAANNNNTTC 4 cut(s) 958, 1160, 1259, 1642
XspI CTAG 2 cut(s) 840, 1661
ZrmI AGTACT 1 cut(s) 715
Zsp2I ATGCAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.