Rroxscaffold_5G00382170

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005491
Physical Location & Seq
Reverse (-)
62012100 .. 62015496
3397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00382170.1

Sequence Viewer

Length: 1458 bp
ATGGCTGGGGAAATAGAAGAACCCTTTTGTTTTAGCTTTCAGCCAAAGTCTATACATTCTGGCTCCATATCATTTGGGAGGTTCGAAAACGAAGGCTTATCTTGGGAAAGGAGGTCTGCTTTCTCACATAACAGATATGTTGAAGAGGTTGAGAAATGCATGAAACCAGGCTCAGTTATTGAGAGGAAAGCTTATTTTGAAGCTCATTTTAAGAAGAAGGGTCTTCCCAGACCAAATTTAGCCGAGTGTTATAGTGGTACATGCCACCAAGTCGGCGAGAATGATGTATCAGAGAGTGATGACGCTTACAGAGAAGAATGTGAACTTGAAAATGAAGGGAGCAGTTATGCTGAATCAGATGACCAAAGGTCGGAGGGTTCTGTGTATCTTGGAGATTTTCAAGTCACAGAATGTAAAAAAGAAGAAGATACTGAACTTCCGTCCTCTGACCCTCAGAGGGCACCTGCATTGAATCATATTGCTATTTTGGTGGATGGTGCTCTTGAAGATGTTAATTTTGAGGAAGCGCATCAACCCGAGAAAGGTTGTGCAAAATCTACTTCGATTGTTGAGGAACTGGAGAGAGATGCGAATGAGAATCATAATGGTGATGCAGTGAATGCTGAGGAGCCGCCTAAGAGTGTTGATATATGTTCAAATACTGAACCAGCTGAGTGTGTTGATAAAACCATTGTGGAAAGTGGGAAAAGTACTTCTTCAAAGTTGAAGGCTGTAAAAGAATCCAAATTTGTAAAGCCTAAGGCCAGCATTTCTCAGGTCCAAAGAAACATTTCTGCTAAGGGATCTGGTAAATATCCTATAGAGAATTCCAATACTGGACAAAGAGAGCGAACACGAAGAACGGATAAAGAAAAGCTCCCATTAAAGACTGCAATGCCAACTAATAAGCATTCTGTACGCAAAACTCCGAAGTTAGAGTTCTTTATGAAGTTGGAAGAAAAGATGCACGCTAAGGAAGATGAAATGAACAAGGTCCAAGCAAGAAAACAGGAAAGGATAGAAGCTGAGACGAAGCAATTCCGGAAAGCCCTCAACTTCAAAGCAGCACCAATGCCTTCTTTCTACAATGTAGCTAGCGCTTCGAGGCCTGATGGGAGCAAGATTGTCTCAACCAAATCCTCTAAAGTAGAAAAAAGCCGAGACAGTGCTGCTGTAAGATCGACATCAGCTTCAGAGTCAGGAAAGAGTGAAGCTGCCACAGGAGAAACTAAATGCAAGAAGGCTAAGGCTTTTGTAACAAAAAGTGAAGCCACTACTGTGCAGAGGAGTCAAGCATTCGAGAGCAGGAAGGTGGCGAAAAATGAGAAAAACGTGGGGAAGCAGAAAGTTGGAGCCCAAAGAAATAGCAGAGAGATGGTGAGGAACGGTACTACGAAAGGCGTGGGCTTTGGAAGCAGTTCCAGAATGAGTCATCTAGCAGTTGGTGTTGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

485

Amino Acids

53.66

Weight (kDa)

8.34

Isoelectric Point (pI)

50.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 313 - 365 1.7e-14 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 472
Acc36I ACCTGC 1 cut(s) 472
AccB1I GGYRCC 1 cut(s) 460
AccIII TCCGGA 1 cut(s) 1041
AciI CCGC 1 cut(s) 632
AclWI GGATC 1 cut(s) 811
AcsI RAATTY 3 cut(s) 235, 746, 826
AcuI CTGAAG 1 cut(s) 1176
AfaI GTAC 4 cut(s) 259, 712, 918, 1390
AfeI AGCGCT 1 cut(s) 1099
AfiI CCNNNNNNNGG 3 cut(s) 370, 457, 542
AhdI GACNNNNNGTC 1 cut(s) 367
AjnI CCWGG 1 cut(s) 166
AleI CACNNNNGTG 1 cut(s) 1277
AluBI AGCT 9 cut(s) 36, 191, 203, 671, 877, 1025, 1094, 1190, 1214
AluI AGCT 9 cut(s) 36, 191, 203, 671, 877, 1025, 1094, 1190, 1214
Alw21I GWGCWC 1 cut(s) 502
Alw26I GTCTC 3 cut(s) 1022, 1132, 1155
AlwI GGATC 1 cut(s) 811
Ama87I CYCGRG 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 1041
Aor51HI AGCGCT 1 cut(s) 1099
AoxI GGCC 2 cut(s) 762, 1106
ApeKI GCWGC 3 cut(s) 1064, 1169, 1214
ApoI RAATTY 3 cut(s) 235, 746, 826
Asp700I GAANNNNTTC 3 cut(s) 790, 1037, 1417
AspLEI GCGC 2 cut(s) 529, 1100
AspS9I GGNCC 2 cut(s) 778, 994
AsuHPI GGTGA 2 cut(s) 620, 1390
AsuII TTCGAA 1 cut(s) 84
AsuNHI GCTAGC 1 cut(s) 1094
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 2 cut(s) 778, 994
AxyI CCTNAGG 1 cut(s) 759
BaeGI GKGCMC 1 cut(s) 463
BanI GGYRCC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 1357
BbsI GAAGAC 1 cut(s) 215
Bbv12I GWGCWC 1 cut(s) 502
BbvCI CCTCAGC 1 cut(s) 624
BbvI GCAGC 3 cut(s) 1076, 1156, 1201
BccI CCATC 3 cut(s) 488, 1106, 1369
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 3 cut(s) 1022, 1132, 1155
BfaI CTAG 2 cut(s) 1095, 1436
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 1101
BfuAI ACCTGC 1 cut(s) 472
BisI GCNGC 4 cut(s) 632, 1065, 1170, 1215
BlsI GCNGC 4 cut(s) 633, 1066, 1171, 1216
BmcAI AGTACT 1 cut(s) 712
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 778, 994
BmeRI GACNNNNNGTC 1 cut(s) 367
BmeT110I CYCGRG 1 cut(s) 536
BmgT120I GGNCC 2 cut(s) 778, 994
BmiI GGNNCC 4 cut(s) 64, 462, 630, 1354
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 4 cut(s) 538, 577, 601, 954
BmtI GCTAGC 1 cut(s) 1098
BpiI GAAGAC 1 cut(s) 215
BpmI CTGGAG 1 cut(s) 599
Bpu10I CCTNAGC 4 cut(s) 624, 798, 972, 1245
Bpu14I TTCGAA 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 1183
BsaWI WCCGGW 1 cut(s) 1041
Bsc4I CCNNNNNNNGG 3 cut(s) 370, 457, 542
Bse1I ACTGG 2 cut(s) 582, 841
Bse21I CCTNAGG 1 cut(s) 759
Bse3DI GCAATG 1 cut(s) 900
Bse8I GATNNNNATC 1 cut(s) 1183
BseAI TCCGGA 1 cut(s) 1041
BseBI CCWGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 499
BseJI GATNNNNATC 1 cut(s) 1183
BseLI CCNNNNNNNGG 3 cut(s) 370, 457, 542
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 6 cut(s) 186, 467, 615, 663, 788, 1017
BseNI ACTGG 2 cut(s) 582, 841
BseRI GAGGAG 2 cut(s) 641, 1300
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 3 cut(s) 1076, 1156, 1201
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 1301
BshFI GGCC 2 cut(s) 764, 1108
BshNI GGYRCC 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 1 cut(s) 1042
BslI CCNNNNNNNGG 3 cut(s) 370, 457, 542
BsmAI GTCTC 3 cut(s) 1022, 1132, 1155
BsmBI CGTCTC 1 cut(s) 1022
BsmI GAATGC 3 cut(s) 625, 910, 1295
BsnI GGCC 2 cut(s) 764, 1108
BsoBI CYCGRG 1 cut(s) 536
Bsp119I TTCGAA 1 cut(s) 84
Bsp1286I GDGCHC 3 cut(s) 463, 502, 1357
Bsp13I TCCGGA 1 cut(s) 1041
Bsp143I GATC 2 cut(s) 803, 1178
BspACI CCGC 1 cut(s) 632
BspANI GGCC 2 cut(s) 764, 1108
BspCNI CTCAG 6 cut(s) 185, 466, 616, 664, 787, 1018
BspEI TCCGGA 1 cut(s) 1041
BspHI TCATGA 1 cut(s) 1454
BspLI GGNNCC 4 cut(s) 64, 462, 630, 1354
BspMI ACCTGC 1 cut(s) 472
BspOI GCTAGC 1 cut(s) 1098
BspPI GGATC 1 cut(s) 811
BspT104I TTCGAA 1 cut(s) 84
BspT107I GGYRCC 1 cut(s) 460
BsrDI GCAATG 1 cut(s) 900
BsrI ACTGG 2 cut(s) 582, 841
BssMI GATC 2 cut(s) 803, 1178
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 3 cut(s) 1166, 1279, 1388
Bst6I CTCTTC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 620
BstBI TTCGAA 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 766, 969, 1096
BstF5I GGATG 1 cut(s) 499
BstH2I RGCGCY 1 cut(s) 1101
BstHHI GCGC 2 cut(s) 529, 1100
BstKTI GATC 2 cut(s) 806, 1181
BstMAI GTCTC 3 cut(s) 1022, 1132, 1155
BstMBI GATC 2 cut(s) 803, 1178
BstMWI GCNNNNNNNGC 2 cut(s) 620, 1413
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 819
BstSLI GKGCMC 1 cut(s) 463
BstV1I GCAGC 3 cut(s) 1076, 1156, 1201
BstV2I GAAGAC 1 cut(s) 215
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
Bsu36I CCTNAGG 1 cut(s) 759
BsuRI GGCC 2 cut(s) 764, 1108
BtsCI GGATG 1 cut(s) 499
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 2 cut(s) 621, 1171
BveI ACCTGC 1 cut(s) 472
Cac8I GCNNGC 3 cut(s) 766, 969, 1096
CciI TCATGA 1 cut(s) 1454
CfoI GCGC 2 cut(s) 529, 1100
Cfr13I GGNCC 2 cut(s) 778, 994
CseI GACGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 258, 711, 917, 1389
CviAII CATG 3 cut(s) 160, 261, 1455
CviQI GTAC 4 cut(s) 258, 711, 917, 1389
DpnI GATC 2 cut(s) 805, 1180
DpnII GATC 2 cut(s) 803, 1178
DriI GACNNNNNGTC 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 138
Eam1105I GACNNNNNGTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 138
Eco147I AGGCCT 1 cut(s) 1108
Eco24I GRGCYC 1 cut(s) 1357
Eco47I GGWCC 2 cut(s) 778, 994
Eco47III AGCGCT 1 cut(s) 1099
Eco57I CTGAAG 1 cut(s) 1176
Eco81I CCTNAGG 1 cut(s) 759
Eco88I CYCGRG 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 826
EcoRII CCWGG 1 cut(s) 166
EcoT22I ATGCAT 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 1357
Esp3I CGTCTC 1 cut(s) 1022
FaeI CATG 3 cut(s) 163, 264, 1458
FalI AAGNNNNNCTT 2 cut(s) 700, 732
FatI CATG 3 cut(s) 159, 260, 1454
Fnu4HI GCNGC 4 cut(s) 632, 1065, 1170, 1215
FokI GGATG 1 cut(s) 506
FriOI GRGCYC 1 cut(s) 1357
Fsp4HI GCNGC 4 cut(s) 632, 1065, 1170, 1215
FspBI CTAG 2 cut(s) 1095, 1436
GlaI GCGC 2 cut(s) 528, 1099
GluI GCNGC 4 cut(s) 632, 1065, 1170, 1215
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 599
HaeII RGCGCY 1 cut(s) 1101
HaeIII GGCC 2 cut(s) 764, 1108
HapII CCGG 1 cut(s) 1042
HgaI GACGC 1 cut(s) 311
HhaI GCGC 2 cut(s) 529, 1100
Hin1II CATG 3 cut(s) 163, 264, 1458
Hin6I GCGC 2 cut(s) 527, 1098
HinP1I GCGC 2 cut(s) 527, 1098
HindIII AAGCTT 1 cut(s) 189
HinfI GANTC 7 cut(s) 353, 472, 598, 740, 1196, 1288, 1429
HpaII CCGG 1 cut(s) 1042
HphI GGTGA 2 cut(s) 620, 1390
Hpy166II GTNNAC 1 cut(s) 323
Hpy188I TCNGA 7 cut(s) 292, 358, 373, 448, 456, 930, 1195
Hpy188III TCNNGA 6 cut(s) 503, 1042, 1200, 1300, 1422, 1455
Hpy8I GTNNAC 1 cut(s) 323
HpyAV CCTTC 7 cut(s) 86, 211, 329, 721, 1086, 1234, 1303
HpyCH4III ACNGT 3 cut(s) 1166, 1279, 1388
HpyCH4IV ACGT 1 cut(s) 1332
HpyCH4V TGCA 8 cut(s) 159, 467, 551, 614, 893, 967, 1236, 1282
HpyF10VI GCNNNNNNNGC 2 cut(s) 620, 1413
HpySE526I ACGT 1 cut(s) 1332
Hsp92II CATG 3 cut(s) 163, 264, 1458
HspAI GCGC 2 cut(s) 527, 1098
Kpn2I TCCGGA 1 cut(s) 1041
Kzo9I GATC 2 cut(s) 803, 1178
LmnI GCTCC 6 cut(s) 68, 339, 628, 882, 1116, 1352
Lsp1109I GCAGC 3 cut(s) 1076, 1156, 1201
LweI GCATC 4 cut(s) 538, 577, 601, 954
MaeI CTAG 2 cut(s) 1095, 1436
MaeII ACGT 1 cut(s) 1332
MaeIII GTNAC 2 cut(s) 403, 1255
MalI GATC 2 cut(s) 805, 1180
MboI GATC 2 cut(s) 803, 1178
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 3 cut(s) 463, 502, 1357
MluCI AATT 5 cut(s) 235, 514, 746, 826, 1037
MlyI GAGTC 3 cut(s) 1205, 1297, 1438
MmeI TCCRAC 3 cut(s) 351, 933, 1330
Mph1103I ATGCAT 1 cut(s) 161
MroI TCCGGA 1 cut(s) 1041
MroXI GAANNNNTTC 3 cut(s) 790, 1037, 1417
MseI TTAA 3 cut(s) 210, 513, 884
MslI CAYNNNNRTG 2 cut(s) 606, 1277
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 1 cut(s) 1042
MspR9I CCNGG 1 cut(s) 168
Mva1269I GAATGC 3 cut(s) 625, 910, 1295
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 620, 1413
NdeII GATC 2 cut(s) 803, 1178
NheI GCTAGC 1 cut(s) 1094
NlaIII CATG 3 cut(s) 163, 264, 1458
NlaIV GGNNCC 4 cut(s) 64, 462, 630, 1354
NmeAIII GCCGAG 2 cut(s) 268, 1184
NmuCI GTSAC 1 cut(s) 403
NsiI ATGCAT 1 cut(s) 161
NspI RCATGY 1 cut(s) 264
NspV TTCGAA 1 cut(s) 84
OliI CACNNNNGTG 1 cut(s) 1277
PagI TCATGA 1 cut(s) 1454
PaqCI CACCTGC 1 cut(s) 472
PceI AGGCCT 1 cut(s) 1108
PctI GAATGC 3 cut(s) 625, 910, 1295
PdmI GAANNNNTTC 3 cut(s) 790, 1037, 1417
PfeI GAWTC 4 cut(s) 353, 472, 598, 740
PkrI GCNGC 4 cut(s) 633, 1066, 1171, 1216
PleI GAGTC 3 cut(s) 1204, 1296, 1437
PpsI GAGTC 3 cut(s) 1204, 1296, 1437
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 4 cut(s) 64, 462, 630, 1354
PspPI GGNCC 2 cut(s) 778, 994
PsuI RGATCY 1 cut(s) 803
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 4 cut(s) 259, 712, 918, 1390
RsaNI GTAC 4 cut(s) 258, 711, 917, 1389
RseI CAYNNNNRTG 2 cut(s) 606, 1277
SaqAI TTAA 3 cut(s) 210, 513, 884
SatI GCNGC 4 cut(s) 632, 1065, 1170, 1215
Sau3AI GATC 2 cut(s) 803, 1178
Sau96I GGNCC 2 cut(s) 778, 994
ScaI AGTACT 1 cut(s) 712
SchI GAGTC 3 cut(s) 1205, 1297, 1438
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 3 cut(s) 463, 502, 1357
SfaNI GCATC 4 cut(s) 538, 577, 601, 954
SfcI CTRYAG 1 cut(s) 819
SfuI TTCGAA 1 cut(s) 84
SinI GGWCC 2 cut(s) 778, 994
SmiMI CAYNNNNRTG 2 cut(s) 606, 1277
Sse9I AATT 5 cut(s) 235, 514, 746, 826, 1037
SseBI AGGCCT 1 cut(s) 1108
SsiI CCGC 1 cut(s) 632
SspMI CTAG 2 cut(s) 1095, 1436
StuI AGGCCT 1 cut(s) 1108
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 3 cut(s) 1166, 1279, 1388
TaiI ACGT 1 cut(s) 1335
TaqI TCGA 5 cut(s) 84, 563, 1103, 1181, 1299
TasI AATT 5 cut(s) 235, 514, 746, 826, 1037
TatI WGTACW 1 cut(s) 710
TauI GCSGC 1 cut(s) 634
TfiI GAWTC 4 cut(s) 353, 472, 598, 740
Tru1I TTAA 3 cut(s) 210, 513, 884
Tru9I TTAA 3 cut(s) 210, 513, 884
TscAI CASTG 2 cut(s) 621, 1171
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 3 cut(s) 1064, 1169, 1214
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 5 cut(s) 176, 348, 962, 996, 1001
TspGWI ACGGA 2 cut(s) 429, 878
TspRI CASTG 2 cut(s) 621, 1171
VpaK11BI GGWCC 2 cut(s) 778, 994
XapI RAATTY 3 cut(s) 235, 746, 826
XceI RCATGY 1 cut(s) 264
XmnI GAANNNNTTC 3 cut(s) 790, 1037, 1417
XspI CTAG 2 cut(s) 1095, 1436
ZrmI AGTACT 1 cut(s) 712
Zsp2I ATGCAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.