RchiOBHm_Chr4g0441291

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0005491
Physical Location & Seq
Reverse (-)
63159076 .. 63162491
3416 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40914

Sequence Viewer

Length: 1677 bp
ATGGCTGGGGAAATAGAAGAACCCTTTTGTTTTAGCTTTCAGCCAAAGTCTATACATTCTGGCTCCATATCATTTGGGAGGTTTGAAAACGAAGGCTTATCTTGGGAAAGGAGGTCTGCTTTCTCACATAACAGATATGTTGAAGAGGTTGAGAAATGCATGAAACCAGGTTCAGTTATTGAGAGGAAAGCTTATTTTGAAGCTCATTTTAAGAAGGGTCTTCCCAGACCAAATTTAGCCGAGTGTTATAGAGGTACATGCTACCAAGTCGGCGAGAATGATGTATCAGAGAGTGATGACGCTTACAGAGAAGAATGTGAACTTGAAAATGAAGGGAGCAGTTATGCTGAATCAGATGACCAAAGGTCGGAGGGTTCTGTGTATCTTGGAGATTTTGAAGTCACAGAATGTGAAAAAGAAGAAGATACTGAACTTCCGTCCTCTGACCCTCAGAGGGCACCTGCATTGAATCATATTGCTATTTTGGTGGATGGTGCTGTTGAAGATGTTAATTTTGAGGAAGCGCATCAACCCGAGAAAGGTTGTGCAAAATCTACTTCGATTGTTGAGGAACTGGAGAGAGATGCAAATGAGAATCATAATGGTGATGCAGTGAATGCTGAGGAGCCGCCTAAGAGTGTTGATATATGTTCAAATACTGAACCAGCTGAGTGTGTTGATAAAACCATTGTGGAAAGTGGGAAAAGTACTTCTTCAAAGTTGAAGGCTGTAAAAGAATCCAAATTTGTAAAGCCTAAGGCCAGCATTTCTCAGGTCCAAAGAAACATTTCTGCTAAGGGATCTGGTATATATCCTATAGAGAATTCCAATACTGGACAAAGAGAGCGTACACGAAGAACGGGTAAAGAAAAGCTCCCATTAAAGACTGCAATGCCAACTAATAAGCATTCTGTTCGCAGAACTCCGAAGTTAGAGGACTCCATGAAGGGAAAATCTTTAAATGAGAGTAAAAGTGTTAAAGAGTCATCACTTAAGAAGCTTGGTGAACTTCAACCTTCTGCATTCAAGCCTGAATCCAGAGGACGCCAAATGACAAACAGGGTTAGTAACGGAGCCAATTTGACTAAGTCTGATGCAAGATCAAGTGTTACAACTTTCAAATTCAAAAGTTCTGAGCGAGCAGAAAAGAGGAAAGAGTTCTTTATGAAGTTGGAAGAAAAAATGCACGCTAAGGAAGATGAAATGAACAAGGTCCAAGCAAGAAAACAGGAAAGGATAGAAGCTGAGACGAAGCAATTCCGGAAAGCCCTCAACTTCAAAGCAGCACCAATGCCTTCTTTCTACAATGTAGCTAGCGCTTCGAGGCCTGATGGGAGCAAGATTTTCTCAACCAAATCCTCTAAAGTAGGAAAAAGCCGAGACAGTGCTGCTGTAAGATCGACATCAGCTTCAGAGTCAGGGAAGAGTGAAGCTGCCACAGGAGAAACTAAATGCAAGATGGCTGAGGCATTTGTAACAAAAAGTGAAGTCACTACTGTGCAGAGGAGTCGAGCATTCGAGAGCAGGAAGGAGGCGAAAAATGAGAAAAACGTGGGGAAGCAGAAAGTTGGAGCCCAAAGAAATAGCAGAGAGATGGTGAGGAAGGGTACTATGAAAGGCGTGGGCTTTGGAAGCAGTTCCAGAATGAGTCATCTAGCAGTTGGTGTTGCCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPX2

Protein Analysis

558

Amino Acids

61.9

Weight (kDa)

8.81

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPX2 PF06886 373 - 438 4.4e-21 Targeting protein for Xklp2 (TPX2) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 469
Acc36I ACCTGC 1 cut(s) 469
AccB1I GGYRCC 1 cut(s) 457
AccIII TCCGGA 1 cut(s) 1260
AciI CCGC 1 cut(s) 629
AclWI GGATC 1 cut(s) 808
AcsI RAATTY 4 cut(s) 232, 743, 823, 1121
AcuI CTGAAG 1 cut(s) 1395
AcyI GRCGYC 1 cut(s) 1045
AfaI GTAC 4 cut(s) 256, 709, 850, 1609
AfeI AGCGCT 1 cut(s) 1318
AfiI CCNNNNNNNGG 3 cut(s) 367, 454, 539
AflII CTTAAG 1 cut(s) 992
AhdI GACNNNNNGTC 1 cut(s) 364
AjnI CCWGG 1 cut(s) 166
AleI CACNNNNGTG 1 cut(s) 1496
Alw26I GTCTC 2 cut(s) 1241, 1374
AlwI GGATC 1 cut(s) 808
Ama87I CYCGRG 1 cut(s) 533
Aor13HI TCCGGA 1 cut(s) 1260
Aor51HI AGCGCT 1 cut(s) 1318
AoxI GGCC 2 cut(s) 759, 1325
ApeKI GCWGC 3 cut(s) 1283, 1388, 1433
ApoI RAATTY 4 cut(s) 232, 743, 823, 1121
Asp700I GAANNNNTTC 4 cut(s) 787, 1157, 1256, 1636
AspLEI GCGC 2 cut(s) 526, 1319
AspS9I GGNCC 2 cut(s) 775, 1213
AsuHPI GGTGA 3 cut(s) 617, 1016, 1609
AsuNHI GCTAGC 1 cut(s) 1313
AvaI CYCGRG 1 cut(s) 533
AvaII GGWCC 2 cut(s) 775, 1213
AxyI CCTNAGG 1 cut(s) 756
BaeGI GKGCMC 1 cut(s) 460
BanI GGYRCC 1 cut(s) 457
BanII GRGCYC 1 cut(s) 1576
BbsI GAAGAC 1 cut(s) 212
BbvCI CCTCAGC 2 cut(s) 621, 1464
BbvI GCAGC 3 cut(s) 1295, 1375, 1420
BccI CCATC 4 cut(s) 485, 1325, 1453, 1588
BcgI CGANNNNNNTGC 4 cut(s) 896, 930, 1490, 1524
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 2 cut(s) 1241, 1374
BfaI CTAG 2 cut(s) 1314, 1655
BfmI CTRYAG 1 cut(s) 816
BfoI RGCGCY 1 cut(s) 1320
BfrI CTTAAG 1 cut(s) 992
BfuAI ACCTGC 1 cut(s) 469
BisI GCNGC 4 cut(s) 629, 1284, 1389, 1434
BlsI GCNGC 4 cut(s) 630, 1285, 1390, 1435
BmcAI AGTACT 1 cut(s) 709
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 775, 1213
BmeRI GACNNNNNGTC 1 cut(s) 364
BmeT110I CYCGRG 1 cut(s) 533
BmgT120I GGNCC 2 cut(s) 775, 1213
BmiI GGNNCC 5 cut(s) 64, 459, 627, 1075, 1573
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 4 cut(s) 535, 574, 598, 1084
BmtI GCTAGC 1 cut(s) 1317
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 596
Bpu10I CCTNAGC 4 cut(s) 621, 795, 1191, 1464
BsaBI GATNNNNATC 1 cut(s) 1402
BsaHI GRCGYC 1 cut(s) 1045
BsaWI WCCGGW 1 cut(s) 1260
Bsc4I CCNNNNNNNGG 3 cut(s) 367, 454, 539
Bse1I ACTGG 2 cut(s) 579, 838
Bse21I CCTNAGG 1 cut(s) 756
Bse3DI GCAATG 1 cut(s) 897
Bse8I GATNNNNATC 1 cut(s) 1402
BseAI TCCGGA 1 cut(s) 1260
BseBI CCWGG 1 cut(s) 168
BseGI GGATG 1 cut(s) 496
BseJI GATNNNNATC 1 cut(s) 1402
BseLI CCNNNNNNNGG 3 cut(s) 367, 454, 539
BseMI GCAATG 1 cut(s) 897
BseMII CTCAG 7 cut(s) 464, 612, 660, 785, 1125, 1236, 1455
BseNI ACTGG 2 cut(s) 579, 838
BseRI GAGGAG 2 cut(s) 638, 1519
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 3 cut(s) 1295, 1375, 1420
BseYI CCCAGC 1 cut(s) 5
BsgI GTGCAG 1 cut(s) 1520
BshFI GGCC 2 cut(s) 761, 1327
BshNI GGYRCC 1 cut(s) 457
BsiHKCI CYCGRG 1 cut(s) 533
BsiSI CCGG 1 cut(s) 1261
BslI CCNNNNNNNGG 3 cut(s) 367, 454, 539
BsmAI GTCTC 2 cut(s) 1241, 1374
BsmBI CGTCTC 1 cut(s) 1241
BsmI GAATGC 4 cut(s) 622, 907, 1022, 1514
BsnI GGCC 2 cut(s) 761, 1327
BsoBI CYCGRG 1 cut(s) 533
Bsp1286I GDGCHC 2 cut(s) 460, 1576
Bsp13I TCCGGA 1 cut(s) 1260
Bsp143I GATC 3 cut(s) 800, 1100, 1397
BspACI CCGC 1 cut(s) 629
BspANI GGCC 2 cut(s) 761, 1327
BspCNI CTCAG 7 cut(s) 463, 613, 661, 784, 1126, 1237, 1456
BspEI TCCGGA 1 cut(s) 1260
BspHI TCATGA 1 cut(s) 1673
BspLI GGNNCC 5 cut(s) 64, 459, 627, 1075, 1573
BspMI ACCTGC 1 cut(s) 469
BspOI GCTAGC 1 cut(s) 1317
BspPI GGATC 1 cut(s) 808
BspT107I GGYRCC 1 cut(s) 457
BspTI CTTAAG 1 cut(s) 992
BsrDI GCAATG 1 cut(s) 897
BsrI ACTGG 2 cut(s) 579, 838
BssMI GATC 3 cut(s) 800, 1100, 1397
BssNI GRCGYC 1 cut(s) 1045
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 1385, 1498
Bst6I CTCTTC 2 cut(s) 138, 1418
BstACI GRCGYC 1 cut(s) 1045
BstAFI CTTAAG 1 cut(s) 992
BstAPI GCANNNNNTGC 1 cut(s) 617
BstC8I GCNNGC 4 cut(s) 763, 1140, 1188, 1315
BstF5I GGATG 1 cut(s) 496
BstH2I RGCGCY 1 cut(s) 1320
BstHHI GCGC 2 cut(s) 526, 1319
BstKTI GATC 3 cut(s) 803, 1103, 1400
BstMAI GTCTC 2 cut(s) 1241, 1374
BstMBI GATC 3 cut(s) 800, 1100, 1397
BstMWI GCNNNNNNNGC 2 cut(s) 617, 1632
BstNI CCWGG 1 cut(s) 168
BstNSI RCATGY 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 816
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 1295, 1375, 1420
BstV2I GAAGAC 1 cut(s) 212
BstX2I RGATCY 1 cut(s) 800
BstYI RGATCY 1 cut(s) 800
Bsu36I CCTNAGG 1 cut(s) 756
BsuRI GGCC 2 cut(s) 761, 1327
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 2 cut(s) 618, 1390
BveI ACCTGC 1 cut(s) 469
Cac8I GCNNGC 4 cut(s) 763, 1140, 1188, 1315
CciI TCATGA 1 cut(s) 1673
CfoI GCGC 2 cut(s) 526, 1319
Cfr13I GGNCC 2 cut(s) 775, 1213
CseI GACGC 2 cut(s) 308, 1053
CsiI ACCWGGT 1 cut(s) 166
Csp6I GTAC 4 cut(s) 255, 708, 849, 1608
CviAII CATG 4 cut(s) 160, 258, 943, 1674
CviQI GTAC 4 cut(s) 255, 708, 849, 1608
DpnI GATC 3 cut(s) 802, 1102, 1399
DpnII GATC 3 cut(s) 800, 1100, 1397
DraI TTTAAA 1 cut(s) 960
DriI GACNNNNNGTC 1 cut(s) 364
Eam1104I CTCTTC 2 cut(s) 138, 1418
Eam1105I GACNNNNNGTC 1 cut(s) 364
EarI CTCTTC 2 cut(s) 138, 1418
Eco147I AGGCCT 1 cut(s) 1327
Eco24I GRGCYC 1 cut(s) 1576
Eco47I GGWCC 2 cut(s) 775, 1213
Eco47III AGCGCT 1 cut(s) 1318
Eco57I CTGAAG 1 cut(s) 1395
Eco81I CCTNAGG 1 cut(s) 756
Eco88I CYCGRG 1 cut(s) 533
EcoRI GAATTC 1 cut(s) 823
EcoRII CCWGG 1 cut(s) 166
EcoT22I ATGCAT 1 cut(s) 161
EcoT38I GRGCYC 1 cut(s) 1576
Esp3I CGTCTC 1 cut(s) 1241
FaeI CATG 4 cut(s) 163, 261, 946, 1677
FalI AAGNNNNNCTT 2 cut(s) 697, 729
FatI CATG 4 cut(s) 159, 257, 942, 1673
Fnu4HI GCNGC 4 cut(s) 629, 1284, 1389, 1434
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 1576
Fsp4HI GCNGC 4 cut(s) 629, 1284, 1389, 1434
FspBI CTAG 2 cut(s) 1314, 1655
GlaI GCGC 2 cut(s) 525, 1318
GluI GCNGC 4 cut(s) 629, 1284, 1389, 1434
GsaI CCCAGC 1 cut(s) 9
GsuI CTGGAG 1 cut(s) 596
HaeII RGCGCY 1 cut(s) 1320
HaeIII GGCC 2 cut(s) 761, 1327
HapII CCGG 1 cut(s) 1261
HgaI GACGC 2 cut(s) 308, 1053
HhaI GCGC 2 cut(s) 526, 1319
Hin1I GRCGYC 1 cut(s) 1045
Hin1II CATG 4 cut(s) 163, 261, 946, 1677
Hin6I GCGC 2 cut(s) 524, 1317
HinP1I GCGC 2 cut(s) 524, 1317
HindIII AAGCTT 2 cut(s) 189, 998
HpaII CCGG 1 cut(s) 1261
HphI GGTGA 3 cut(s) 617, 1016, 1609
Hpy166II GTNNAC 3 cut(s) 320, 851, 1007
Hpy188I TCNGA 9 cut(s) 289, 355, 370, 445, 453, 927, 1093, 1135, 1414
Hpy188III TCNNGA 5 cut(s) 1038, 1261, 1519, 1641, 1674
Hpy8I GTNNAC 3 cut(s) 320, 851, 1007
HpyAV CCTTC 9 cut(s) 86, 208, 326, 718, 940, 1026, 1305, 1522, 1597
HpyCH4III ACNGT 2 cut(s) 1385, 1498
HpyCH4IV ACGT 1 cut(s) 1551
HpyF10VI GCNNNNNNNGC 2 cut(s) 617, 1632
HpySE526I ACGT 1 cut(s) 1551
Hsp92I GRCGYC 1 cut(s) 1045
Hsp92II CATG 4 cut(s) 163, 261, 946, 1677
HspAI GCGC 2 cut(s) 524, 1317
Kpn2I TCCGGA 1 cut(s) 1260
Kzo9I GATC 3 cut(s) 800, 1100, 1397
LmnI GCTCC 7 cut(s) 68, 336, 625, 879, 1073, 1335, 1571
Lsp1109I GCAGC 3 cut(s) 1295, 1375, 1420
LweI GCATC 4 cut(s) 535, 574, 598, 1084
MabI ACCWGGT 1 cut(s) 166
MaeI CTAG 2 cut(s) 1314, 1655
MaeII ACGT 1 cut(s) 1551
MaeIII GTNAC 5 cut(s) 400, 1067, 1108, 1474, 1489
MalI GATC 3 cut(s) 802, 1102, 1399
MboI GATC 3 cut(s) 800, 1100, 1397
MflI RGATCY 1 cut(s) 800
MhlI GDGCHC 2 cut(s) 460, 1576
MluCI AATT 7 cut(s) 232, 511, 743, 823, 1078, 1121, 1256
MlyI GAGTC 5 cut(s) 932, 992, 1424, 1516, 1657
MmeI TCCRAC 3 cut(s) 348, 1152, 1549
Mph1103I ATGCAT 1 cut(s) 161
MroI TCCGGA 1 cut(s) 1260
MroXI GAANNNNTTC 4 cut(s) 787, 1157, 1256, 1636
MseI TTAA 6 cut(s) 210, 510, 881, 959, 978, 993
MslI CAYNNNNRTG 2 cut(s) 603, 1496
MspA1I CMGCKG 1 cut(s) 668
MspCI CTTAAG 1 cut(s) 992
MspI CCGG 1 cut(s) 1261
MspR9I CCNGG 1 cut(s) 168
Mva1269I GAATGC 4 cut(s) 622, 907, 1022, 1514
MvaI CCWGG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 617, 1632
NdeII GATC 3 cut(s) 800, 1100, 1397
NheI GCTAGC 1 cut(s) 1313
NlaIII CATG 4 cut(s) 163, 261, 946, 1677
NlaIV GGNNCC 5 cut(s) 64, 459, 627, 1075, 1573
NmeAIII GCCGAG 2 cut(s) 265, 1403
NmuCI GTSAC 2 cut(s) 400, 1489
NsiI ATGCAT 1 cut(s) 161
NspI RCATGY 1 cut(s) 261
OliI CACNNNNGTG 1 cut(s) 1496
PagI TCATGA 1 cut(s) 1673
PaqCI CACCTGC 1 cut(s) 469
PceI AGGCCT 1 cut(s) 1327
PctI GAATGC 4 cut(s) 622, 907, 1022, 1514
PdmI GAANNNNTTC 4 cut(s) 787, 1157, 1256, 1636
PfeI GAWTC 5 cut(s) 350, 469, 595, 737, 1034
PflFI GACNNNGTC 1 cut(s) 1087
PkrI GCNGC 4 cut(s) 630, 1285, 1390, 1435
PleI GAGTC 5 cut(s) 932, 991, 1423, 1515, 1656
PpsI GAGTC 5 cut(s) 932, 991, 1423, 1515, 1656
Psp6I CCWGG 1 cut(s) 166
PspFI CCCAGC 1 cut(s) 5
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 5 cut(s) 64, 459, 627, 1075, 1573
PspPI GGNCC 2 cut(s) 775, 1213
PsuI RGATCY 1 cut(s) 800
PsyI GACNNNGTC 1 cut(s) 1087
PvuII CAGCTG 1 cut(s) 668
RsaI GTAC 4 cut(s) 256, 709, 850, 1609
RsaNI GTAC 4 cut(s) 255, 708, 849, 1608
RseI CAYNNNNRTG 2 cut(s) 603, 1496
SaqAI TTAA 6 cut(s) 210, 510, 881, 959, 978, 993
SatI GCNGC 4 cut(s) 629, 1284, 1389, 1434
Sau3AI GATC 3 cut(s) 800, 1100, 1397
Sau96I GGNCC 2 cut(s) 775, 1213
ScaI AGTACT 1 cut(s) 709
SchI GAGTC 5 cut(s) 932, 992, 1424, 1516, 1657
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 2 cut(s) 460, 1576
SexAI ACCWGGT 1 cut(s) 166
SfaNI GCATC 4 cut(s) 535, 574, 598, 1084
SfcI CTRYAG 1 cut(s) 816
SinI GGWCC 2 cut(s) 775, 1213
SmiMI CAYNNNNRTG 2 cut(s) 603, 1496
SmlI CTYRAG 1 cut(s) 992
SmoI CTYRAG 1 cut(s) 992
Sse9I AATT 7 cut(s) 232, 511, 743, 823, 1078, 1121, 1256
SseBI AGGCCT 1 cut(s) 1327
SsiI CCGC 1 cut(s) 629
SspMI CTAG 2 cut(s) 1314, 1655
StuI AGGCCT 1 cut(s) 1327
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 1385, 1498
TaiI ACGT 1 cut(s) 1554
TaqI TCGA 5 cut(s) 560, 1322, 1400, 1510, 1518
TasI AATT 7 cut(s) 232, 511, 743, 823, 1078, 1121, 1256
TatI WGTACW 1 cut(s) 707
TauI GCSGC 1 cut(s) 631
TfiI GAWTC 5 cut(s) 350, 469, 595, 737, 1034
Tru1I TTAA 6 cut(s) 210, 510, 881, 959, 978, 993
Tru9I TTAA 6 cut(s) 210, 510, 881, 959, 978, 993
TscAI CASTG 2 cut(s) 618, 1390
TseFI GTSAC 2 cut(s) 400, 1489
TseI GCWGC 3 cut(s) 1283, 1388, 1433
Tsp45I GTSAC 2 cut(s) 400, 1489
TspDTI ATGAA 7 cut(s) 176, 345, 959, 1181, 1215, 1220, 1628
TspGWI ACGGA 2 cut(s) 426, 1086
TspRI CASTG 2 cut(s) 618, 1390
Tth111I GACNNNGTC 1 cut(s) 1087
Vha464I CTTAAG 1 cut(s) 992
VpaK11BI GGWCC 2 cut(s) 775, 1213
XapI RAATTY 4 cut(s) 232, 743, 823, 1121
XceI RCATGY 1 cut(s) 261
XmnI GAANNNNTTC 4 cut(s) 787, 1157, 1256, 1636
XspI CTAG 2 cut(s) 1314, 1655
ZrmI AGTACT 1 cut(s) 709
Zsp2I ATGCAT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.