AT1G71250

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016165
Physical Location & Seq
Forward (+)
26860110 .. 26861726
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G71250.1

Sequence Viewer

Length: 1125 bp
ATGAACACAAACAGAAAGAAGATGAAAGTCCATATTGGAGGATATGTGTTGATACTTGCCTTGACGGTTTCTGTTATATTACAGCAGCCTGAGTTGGTCACTGGACAAGCTAGAGTTCCTGCTATGTTTGTACTTGGAGACTCATTGGTCGATGCTGGTAACAACAACTTTCTACAAACTGTAGCTCGAGCCAATTTCCTCCCTTATGGCATCGACATGAACTACCAGCCTACTGGCAGATTTAGCAACGGCCTGACTTTCATTGATCTGCTAGCTCGGTTGTTAGAAATTCCATCACCCCCTCCCTTCGCGGATCCAACCACATCAGGCAATAGAATCCTCCAAGGAGTTAACTACGCGTCTGCAGCTGCTGGCATCCTCGATGTATCAGGCTACAACTATGGTGGAAGATTCAGCCTGAACCAGCAAATGGTGAATCTAGAGACGACACTAAGCCAACTGCGAACGATGATGAGTCCCCAAAACTTTACGGATTACTTAGCAAGGTCGCTTGTGGTTCTCGTTTTTGGAAGCAATGACTATATCAACAACTACCTTATGCCTAATCTCTACGATTCCAGCATAAGATTCAGACCACCTGACTTTGCCAATCTACTTCTCAGTCAATACGCTCGCCAGCTTCTGACTCTTTACAGCCTAGGTCTAAGAAAAATATTTATACCTGGAGTAGCACCACTAGGCTGCATACCAAACCAACGAGCCAGAGGTATCTCGCCACCTGATAGATGTGTTGACAGCGTGAATCAGATTCTAGGCACATTTAACCAAGGGCTAAAGTCACTTGTTGATCAGCTGAACCAGCGATCACCTGGAGCCATCTATGTATACGGCAACACCTATAGCGCAATTGGTGACATCTTAAACAACCCAGCTGCTTACGGATTTAGCGTAGTAGACAGGGCTTGTTGCGGGATTGGGAGGAACCAAGGACAAATTACATGTCTGCCGTTGCAGACTCCTTGTCCCAACAGAAATCAGTATGTCTTTTGGGATGCATTCCATCCTACTCAGACTGCTAACTCCATTCTGGCTAGACGAGCTTTCTATGGTCCACCTTCCGACGCATATCCTGTCAATGTCCAACAGATGACACTCCTTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

374

Amino Acids

41.19

Weight (kDa)

9.1

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 353 1.8e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71250
fragaria_vesca FvH4_2g00180
malus_domestica MD10G1000500.v1.1
prunus_persica Prupe.8G001600_v2.0.a1 Prupe.8G001600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249471
rosa_laevigata RLG00000015578
rosa_multiflora Rmu_ssc0000459.1_g000021
rosa_roxburghii Rroxscaffold_7G00217670
rosa_rugosa Rorug05G0492600
rosa_samantha Rh6AG002300 Rh6BG007800 Rh6CG001500 Rh6DG001500
rosa_wichuraiana Rw6G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 430
AccI GTMKAC 2 cut(s) 846, 915
AccII CGCG 2 cut(s) 311, 359
AciI CCGC 2 cut(s) 311, 930
AclWI GGATC 2 cut(s) 308, 321
AcsI RAATTY 1 cut(s) 288
AfaI GTAC 1 cut(s) 132
AfiI CCNNNNNNNGG 1 cut(s) 430
AflIII ACRYGT 2 cut(s) 357, 959
AhdI GACNNNNNGTC 1 cut(s) 981
AjnI CCWGG 2 cut(s) 682, 829
AluBI AGCT 8 cut(s) 110, 185, 275, 368, 640, 814, 893, 1061
AluI AGCT 8 cut(s) 110, 185, 275, 368, 640, 814, 893, 1061
Alw26I GTCTC 2 cut(s) 132, 437
AlwI GGATC 2 cut(s) 308, 321
AlwNI CAGNNNCTG 2 cut(s) 371, 643
Ama87I CYCGRG 1 cut(s) 186
AoxI GGCC 1 cut(s) 250
ApeKI GCWGC 5 cut(s) 85, 365, 368, 702, 893
ApoI RAATTY 1 cut(s) 288
ArsI GACNNNNNNTTYG 2 cut(s) 946, 978
AspA2I CCTAGG 1 cut(s) 658
AspLEI GCGC 1 cut(s) 866
AspS9I GGNCC 1 cut(s) 1070
AsuHPI GGTGA 4 cut(s) 288, 445, 819, 884
AsuNHI GCTAGC 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 1 cut(s) 1070
AvrII CCTAGG 1 cut(s) 658
BamHI GGATCC 1 cut(s) 313
BbvI GCAGC 5 cut(s) 97, 355, 377, 689, 880
BccI CCATC 3 cut(s) 301, 845, 1029
BceAI ACGGC 3 cut(s) 265, 865, 952
BciT130I CCWGG 2 cut(s) 684, 831
BclI TGATCA 1 cut(s) 808
BcoDI GTCTC 2 cut(s) 132, 437
BfaI CTAG 8 cut(s) 111, 272, 440, 659, 698, 773, 1053, 1123
BfmI CTRYAG 3 cut(s) 180, 363, 859
BisI GCNGC 5 cut(s) 86, 366, 369, 703, 894
BlnI CCTAGG 1 cut(s) 658
BlsI GCNGC 5 cut(s) 87, 367, 370, 704, 895
Bme1390I CCNGG 2 cut(s) 684, 831
Bme18I GGWCC 1 cut(s) 1070
BmeRI GACNNNNNGTC 1 cut(s) 981
BmeT110I CYCGRG 1 cut(s) 186
BmgT120I GGNCC 1 cut(s) 1070
BmiI GGNNCC 3 cut(s) 315, 835, 944
BmrFI CCNGG 2 cut(s) 684, 831
BmsI GCATC 4 cut(s) 142, 219, 384, 1003
BmtI GCTAGC 1 cut(s) 275
BpmI CTGGAG 2 cut(s) 705, 852
BsaJI CCNNGG 4 cut(s) 343, 658, 787, 946
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 1 cut(s) 430
Bse1I ACTGG 2 cut(s) 106, 238
Bse3DI GCAATG 1 cut(s) 541
BseBI CCWGG 2 cut(s) 684, 831
BseDI CCNNGG 4 cut(s) 343, 658, 787, 946
BseGI GGATG 3 cut(s) 375, 1018, 1021
BseLI CCNNNNNNNGG 1 cut(s) 430
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 3 cut(s) 81, 634, 1043
BseNI ACTGG 2 cut(s) 106, 238
BseXI GCAGC 5 cut(s) 97, 355, 377, 689, 880
BseYI CCCAGC 1 cut(s) 889
Bsh1236I CGCG 2 cut(s) 311, 359
BshFI GGCC 1 cut(s) 252
BsiHKCI CYCGRG 1 cut(s) 186
BslFI GGGAC 2 cut(s) 462, 969
BslI CCNNNNNNNGG 1 cut(s) 430
BsmAI GTCTC 2 cut(s) 132, 437
BsmBI CGTCTC 1 cut(s) 437
BsmFI GGGAC 2 cut(s) 462, 969
BsmI GAATGC 1 cut(s) 1016
BsnI GGCC 1 cut(s) 252
BsoBI CYCGRG 1 cut(s) 186
Bsp143I GATC 4 cut(s) 265, 313, 808, 824
BspACI CCGC 2 cut(s) 311, 930
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 3 cut(s) 82, 633, 1042
BspFNI CGCG 2 cut(s) 311, 359
BspLI GGNNCC 3 cut(s) 315, 835, 944
BspMAI CTGCAG 1 cut(s) 367
BspOI GCTAGC 1 cut(s) 275
BspPI GGATC 2 cut(s) 308, 321
BsrDI GCAATG 1 cut(s) 541
BsrI ACTGG 2 cut(s) 106, 238
BssECI CCNNGG 4 cut(s) 343, 658, 787, 946
BssMI GATC 4 cut(s) 265, 313, 808, 824
BssNAI GTATAC 1 cut(s) 847
BssT1I CCWWGG 4 cut(s) 343, 658, 787, 946
Bst1107I GTATAC 1 cut(s) 847
Bst2UI CCWGG 2 cut(s) 684, 831
Bst4CI ACNGT 2 cut(s) 67, 181
BstC8I GCNNGC 4 cut(s) 273, 373, 634, 638
BstDEI CTNAG 6 cut(s) 90, 452, 499, 620, 665, 1029
BstF5I GGATG 3 cut(s) 375, 1018, 1021
BstFNI CGCG 2 cut(s) 311, 359
BstHHI GCGC 1 cut(s) 866
BstKTI GATC 4 cut(s) 268, 316, 811, 827
BstMAI GTCTC 2 cut(s) 132, 437
BstMBI GATC 4 cut(s) 265, 313, 808, 824
BstMWI GCNNNNNNNGC 4 cut(s) 243, 365, 820, 1058
BstNI CCWGG 2 cut(s) 684, 831
BstNSI RCATGY 1 cut(s) 963
BstSCI CCNGG 2 cut(s) 682, 829
BstSFI CTRYAG 3 cut(s) 180, 363, 859
BstUI CGCG 2 cut(s) 311, 359
BstV1I GCAGC 5 cut(s) 97, 355, 377, 689, 880
BstX2I RGATCY 1 cut(s) 313
BstXI CCANNNNNNTGG 1 cut(s) 233
BstYI RGATCY 1 cut(s) 313
BstZ17I GTATAC 1 cut(s) 847
BsuRI GGCC 1 cut(s) 252
BtsCI GGATG 3 cut(s) 375, 1018, 1021
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 4 cut(s) 273, 373, 634, 638
CaiI CAGNNNCTG 2 cut(s) 371, 643
CfoI GCGC 1 cut(s) 866
Cfr13I GGNCC 1 cut(s) 1070
CseI GACGC 2 cut(s) 348, 1091
Csp6I GTAC 1 cut(s) 131
CspCI CAANNNNNGTGG 2 cut(s) 385, 420
CviAII CATG 2 cut(s) 217, 960
CviQI GTAC 1 cut(s) 131
DdeI CTNAG 6 cut(s) 90, 452, 499, 620, 665, 1029
DpnI GATC 4 cut(s) 267, 315, 810, 826
DpnII GATC 4 cut(s) 265, 313, 808, 824
DrdI GACNNNNNNGTC 1 cut(s) 146
DriI GACNNNNNGTC 1 cut(s) 981
DseDI GACNNNNNNGTC 1 cut(s) 146
Eam1105I GACNNNNNGTC 1 cut(s) 981
Eco130I CCWWGG 4 cut(s) 343, 658, 787, 946
Eco47I GGWCC 1 cut(s) 1070
Eco88I CYCGRG 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 682, 829
EcoT14I CCWWGG 4 cut(s) 343, 658, 787, 946
EcoT22I ATGCAT 1 cut(s) 1018
ErhI CCWWGG 4 cut(s) 343, 658, 787, 946
Esp3I CGTCTC 1 cut(s) 437
FaeI CATG 2 cut(s) 220, 963
FaqI GGGAC 2 cut(s) 462, 969
FatI CATG 2 cut(s) 216, 959
FauI CCCGC 1 cut(s) 923
FbaI TGATCA 1 cut(s) 808
FblI GTMKAC 2 cut(s) 846, 915
Fnu4HI GCNGC 5 cut(s) 86, 366, 369, 703, 894
FokI GGATG 3 cut(s) 362, 1008, 1025
Fsp4HI GCNGC 5 cut(s) 86, 366, 369, 703, 894
FspBI CTAG 8 cut(s) 111, 272, 440, 659, 698, 773, 1053, 1123
GlaI GCGC 1 cut(s) 865
GluI GCNGC 5 cut(s) 86, 366, 369, 703, 894
GsaI CCCAGC 1 cut(s) 893
GsuI CTGGAG 2 cut(s) 705, 852
HaeIII GGCC 1 cut(s) 252
HgaI GACGC 2 cut(s) 348, 1091
HhaI GCGC 1 cut(s) 866
Hin1II CATG 2 cut(s) 220, 963
Hin6I GCGC 1 cut(s) 864
HinP1I GCGC 1 cut(s) 864
HincII GTYRAC 2 cut(s) 352, 754
HindII GTYRAC 2 cut(s) 352, 754
HpaI GTTAAC 1 cut(s) 352
HphI GGTGA 4 cut(s) 288, 445, 819, 884
Hpy166II GTNNAC 5 cut(s) 352, 754, 847, 916, 1073
Hpy188I TCNGA 5 cut(s) 593, 645, 768, 1032, 1081
Hpy188III TCNNGA 1 cut(s) 440
Hpy8I GTNNAC 5 cut(s) 352, 754, 847, 916, 1073
Hpy99I CGWCG 1 cut(s) 1085
HpyAV CCTTC 2 cut(s) 316, 1086
HpyCH4III ACNGT 2 cut(s) 67, 181
HpyCH4V TGCA 4 cut(s) 365, 705, 973, 1016
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 365, 820, 1058
HpyF3I CTNAG 6 cut(s) 90, 452, 499, 620, 665, 1029
Hsp92II CATG 2 cut(s) 220, 963
HspAI GCGC 1 cut(s) 864
Ksp22I TGATCA 1 cut(s) 808
KspAI GTTAAC 1 cut(s) 352
Kzo9I GATC 4 cut(s) 265, 313, 808, 824
LmnI GCTCC 1 cut(s) 833
Lsp1109I GCAGC 5 cut(s) 97, 355, 377, 689, 880
LweI GCATC 4 cut(s) 142, 219, 384, 1003
MaeI CTAG 8 cut(s) 111, 272, 440, 659, 698, 773, 1053, 1123
MaeIII GTNAC 4 cut(s) 97, 158, 798, 872
MalI GATC 4 cut(s) 267, 315, 810, 826
MboI GATC 4 cut(s) 265, 313, 808, 824
MboII GAAGA 2 cut(s) 31, 420
MfeI CAATTG 1 cut(s) 867
MflI RGATCY 1 cut(s) 313
MluCI AATT 4 cut(s) 193, 288, 867, 954
MluI ACGCGT 1 cut(s) 357
MlyI GAGTC 4 cut(s) 134, 484, 640, 970
MmeI TCCRAC 2 cut(s) 341, 1104
MnlI CCTC 7 cut(s) 32, 209, 312, 350, 389, 719, 933
Mph1103I ATGCAT 1 cut(s) 1018
MseI TTAA 3 cut(s) 351, 783, 881
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 3 cut(s) 368, 814, 893
MspR9I CCNGG 2 cut(s) 684, 831
MunI CAATTG 1 cut(s) 867
Mva1269I GAATGC 1 cut(s) 1016
MvaI CCWGG 2 cut(s) 684, 831
MvnI CGCG 2 cut(s) 311, 359
MwoI GCNNNNNNNGC 4 cut(s) 243, 365, 820, 1058
NdeII GATC 4 cut(s) 265, 313, 808, 824
NheI GCTAGC 1 cut(s) 271
NlaIII CATG 2 cut(s) 220, 963
NlaIV GGNNCC 3 cut(s) 315, 835, 944
NmuCI GTSAC 3 cut(s) 97, 798, 872
NsiI ATGCAT 1 cut(s) 1018
NspI RCATGY 1 cut(s) 963
PaeR7I CTCGAG 1 cut(s) 186
PciI ACATGT 1 cut(s) 959
PcsI WCGNNNNNNNCGW 1 cut(s) 906
PctI GAATGC 1 cut(s) 1016
PfeI GAWTC 7 cut(s) 336, 411, 436, 575, 588, 763, 769
PflMI CCANNNNNTGG 1 cut(s) 430
PkrI GCNGC 5 cut(s) 87, 367, 370, 704, 895
PleI GAGTC 4 cut(s) 134, 483, 640, 970
PpsI GAGTC 4 cut(s) 134, 483, 640, 970
PscI ACATGT 1 cut(s) 959
Psp6I CCWGG 2 cut(s) 682, 829
PspFI CCCAGC 1 cut(s) 889
PspGI CCWGG 2 cut(s) 682, 829
PspN4I GGNNCC 3 cut(s) 315, 835, 944
PspPI GGNCC 1 cut(s) 1070
PspXI VCTCGAGB 1 cut(s) 186
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 2 cut(s) 371, 643
PsuI RGATCY 1 cut(s) 313
PvuII CAGCTG 3 cut(s) 368, 814, 893
RsaI GTAC 1 cut(s) 132
RsaNI GTAC 1 cut(s) 131
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 3 cut(s) 351, 783, 881
SatI GCNGC 5 cut(s) 86, 366, 369, 703, 894
Sau3AI GATC 4 cut(s) 265, 313, 808, 824
Sau96I GGNCC 1 cut(s) 1070
SchI GAGTC 4 cut(s) 134, 484, 640, 970
ScrFI CCNGG 2 cut(s) 684, 831
SfaNI GCATC 4 cut(s) 142, 219, 384, 1003
SfcI CTRYAG 3 cut(s) 180, 363, 859
Sfr274I CTCGAG 1 cut(s) 186
SinI GGWCC 1 cut(s) 1070
SlaI CTCGAG 1 cut(s) 186
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 1 cut(s) 186
SmoI CTYRAG 1 cut(s) 186
Sse9I AATT 4 cut(s) 193, 288, 867, 954
SsiI CCGC 2 cut(s) 311, 930
SspI AATATT 1 cut(s) 675
SspMI CTAG 8 cut(s) 111, 272, 440, 659, 698, 773, 1053, 1123
StyD4I CCNGG 2 cut(s) 682, 829
StyI CCWWGG 4 cut(s) 343, 658, 787, 946
TaaI ACNGT 2 cut(s) 67, 181
TaqI TCGA 4 cut(s) 150, 187, 213, 381
TasI AATT 4 cut(s) 193, 288, 867, 954
TatI WGTACW 1 cut(s) 130
TfiI GAWTC 7 cut(s) 336, 411, 436, 575, 588, 763, 769
Tru1I TTAA 3 cut(s) 351, 783, 881
Tru9I TTAA 3 cut(s) 351, 783, 881
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 3 cut(s) 97, 798, 872
TseI GCWGC 5 cut(s) 85, 365, 368, 702, 893
Tsp45I GTSAC 3 cut(s) 97, 798, 872
TspDTI ATGAA 4 cut(s) 17, 38, 233, 250
TspGWI ACGGA 2 cut(s) 506, 915
TspRI CASTG 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 430
VpaK11BI GGWCC 1 cut(s) 1070
XapI RAATTY 1 cut(s) 288
XbaI TCTAGA 1 cut(s) 439
XceI RCATGY 1 cut(s) 963
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XhoI CTCGAG 1 cut(s) 186
XmaJI CCTAGG 1 cut(s) 658
XmiI GTMKAC 2 cut(s) 846, 915
XspI CTAG 8 cut(s) 111, 272, 440, 659, 698, 773, 1053, 1123
Zsp2I ATGCAT 1 cut(s) 1018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.