Rmu_ssc0000459.1_g000021

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000459.1
OG0016165
Physical Location & Seq
Forward (+)
130125 .. 131698
1574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000459.1_g000021.1.cds

Sequence Viewer

Length: 1161 bp
atgggtggtaagaagatggtagcgatttgtaatgaccggctgtggttagccaattatactatcggaatggtgtttacaattatcttaattaatagtagtatggtggccggcatgggatttggggagtcagccgcggcggcggcggcggcgcaggttccggcaatgtttgcgtttggggactcactcattgatgctggcaacaacaacttcataagctccatcgcaaaatccaattattatccttacggcatcgatttcaggggaggcccctccggcagattcaccaatggaagaactttcgttgatatgcttggaaatttgctgggccttccttatgctccagcctttgcagatcctaatacaaaaggaaacagaatactcaggggagtcaattatgcttcagcagctgctggcatactcgatgagacaggccggcactatggagatcggtataccctaagccagcaagtgttgaatttcgagaccaccttgaatcagttaagaacgatgatggggggaactaacctgacccagtacctggctaagtcgattgccgttttggtatttgggagcaatgactacatcaacaactacctcatgccttccttctattcttccagctacacttacactcctcctgcctttgccaaccttctccttaatcgctatgccacacagatcctggctttgcacggtcttggattgaggaagtttgtgctagtaggaattgggcctttgggttgcattcctaaccaaagagcatcaggtaaggcccagcctggaaggtgtgttgacaatgtcaatcagatccttggcagcttcaatcaagggctcagatcgctcgtcacccaacttaacaacaaccatcctggtgccatctttgtgtacggcaatgcttatggcgcttttggtgacattctgaataaccctgcagcctatggtttcagtgtgattgatagggcttgctgtggaattgggaggaaccaagggcagataacttgtcttccatttgcagttccatgctcaaatagaaatcaatacatgttttgggatgctttccacccaactgaagctgcaaacgcagtccttgcttggcgagcttttaatggcccgccctctgatagttaccccatcaatgtccaacaaatggctctcatataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

386

Amino Acids

41.64

Weight (kDa)

9.0

Isoelectric Point (pI)

27.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71250
fragaria_vesca FvH4_2g00180
malus_domestica MD10G1000500.v1.1
prunus_persica Prupe.8G001600_v2.0.a1 Prupe.8G001600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249471
rosa_laevigata RLG00000015578
rosa_multiflora Rmu_ssc0000459.1_g000021
rosa_roxburghii Rroxscaffold_7G00217670
rosa_rugosa Rorug05G0492600
rosa_samantha Rh6AG002300 Rh6BG007800 Rh6CG001500 Rh6DG001500
rosa_wichuraiana Rw6G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 142
AccB1I GGYRCC 1 cut(s) 872
AccB7I CCANNNNNTGG 2 cut(s) 538, 1147
AccI GTMKAC 1 cut(s) 452
AccII CGCG 1 cut(s) 134
AciI CCGC 7 cut(s) 132, 134, 137, 140, 143, 146, 1112
AclWI GGATC 3 cut(s) 347, 673, 802
AcoI YGGCCR 1 cut(s) 105
AcsI RAATTY 2 cut(s) 316, 475
AcuI CTGAAG 2 cut(s) 384, 1089
AfaI GTAC 2 cut(s) 536, 887
AfiI CCNNNNNNNGG 2 cut(s) 538, 1147
AflIII ACRYGT 1 cut(s) 1041
AgsI TTSAA 3 cut(s) 475, 493, 823
AjnI CCWGG 4 cut(s) 537, 681, 778, 868
AluBI AGCT 6 cut(s) 216, 407, 621, 819, 1073, 1100
AluI AGCT 6 cut(s) 216, 407, 621, 819, 1073, 1100
Alw26I GTCTC 2 cut(s) 419, 476
AlwI GGATC 3 cut(s) 347, 673, 802
AlwNI CAGNNNCTG 4 cut(s) 407, 410, 538, 682
AoxI GGCC 7 cut(s) 105, 265, 325, 430, 731, 771, 1108
ApeKI GCWGC 5 cut(s) 404, 407, 816, 932, 1073
ApoI RAATTY 2 cut(s) 316, 475
AseI ATTAAT 1 cut(s) 90
AspLEI GCGC 2 cut(s) 151, 905
AspS9I GGNCC 5 cut(s) 266, 325, 731, 772, 1109
AsuHPI GGTGA 3 cut(s) 274, 838, 923
BanI GGYRCC 1 cut(s) 872
BanII GRGCYC 1 cut(s) 834
BbsI GAAGAC 1 cut(s) 995
BbvI GCAGC 5 cut(s) 394, 416, 828, 944, 1060
BccI CCATC 6 cut(s) 10, 227, 505, 873, 884, 1139
BceAI ACGGC 3 cut(s) 262, 539, 904
BciT130I CCWGG 4 cut(s) 539, 683, 780, 870
BcoDI GTCTC 2 cut(s) 419, 476
BfaI CTAG 1 cut(s) 719
BfmI CTRYAG 1 cut(s) 930
BfoI RGCGCY 1 cut(s) 906
BfuAI ACCTGC 1 cut(s) 142
BglI GCCNNNNNGGC 2 cut(s) 137, 273
Bme1390I CCNGG 4 cut(s) 539, 683, 780, 870
BmgT120I GGNCC 5 cut(s) 266, 325, 731, 772, 1109
BmiI GGNNCC 4 cut(s) 156, 268, 874, 983
BmrFI CCNGG 4 cut(s) 539, 683, 780, 870
BmrI ACTGGG 1 cut(s) 526
BmsI GCATC 4 cut(s) 181, 258, 770, 1042
BmuI ACTGGG 1 cut(s) 526
BpiI GAAGAC 1 cut(s) 995
BpmI CTGGAG 1 cut(s) 324
Bpu10I CCTNAGC 1 cut(s) 458
Bsa29I ATCGAT 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 3 cut(s) 132, 811, 985
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 2 cut(s) 538, 1147
Bse118I RCCGGY 3 cut(s) 36, 107, 432
Bse1I ACTGG 1 cut(s) 532
Bse3DI GCAATG 3 cut(s) 168, 580, 898
BseBI CCWGG 4 cut(s) 539, 683, 780, 870
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 3 cut(s) 132, 811, 985
BseGI GGATG 2 cut(s) 865, 1057
BseLI CCNNNNNNNGG 2 cut(s) 538, 1147
BseMI GCAATG 3 cut(s) 168, 580, 898
BseMII CTCAG 2 cut(s) 394, 847
BseNI ACTGG 1 cut(s) 532
BseRI GAGGAG 1 cut(s) 624
BseXI GCAGC 5 cut(s) 394, 416, 828, 944, 1060
BseYI CCCAGC 2 cut(s) 322, 774
Bsh1236I CGCG 1 cut(s) 134
BshFI GGCC 7 cut(s) 107, 267, 327, 432, 733, 773, 1110
BshNI GGYRCC 1 cut(s) 872
BshVI ATCGAT 1 cut(s) 252
BsiSI CCGG 5 cut(s) 37, 108, 158, 273, 433
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 2 cut(s) 538, 1147
BsmAI GTCTC 2 cut(s) 419, 476
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 744
BsnI GGCC 7 cut(s) 107, 267, 327, 432, 733, 773, 1110
Bso31I GGTCTC 1 cut(s) 476
Bsp1286I GDGCHC 1 cut(s) 834
Bsp143I GATC 5 cut(s) 352, 445, 678, 807, 836
BspACI CCGC 7 cut(s) 132, 134, 137, 140, 143, 146, 1112
BspANI GGCC 7 cut(s) 107, 267, 327, 432, 733, 773, 1110
BspCNI CTCAG 2 cut(s) 393, 846
BspDI ATCGAT 1 cut(s) 252
BspFNI CGCG 1 cut(s) 134
BspLI GGNNCC 4 cut(s) 156, 268, 874, 983
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 1 cut(s) 142
BspPI GGATC 3 cut(s) 347, 673, 802
BspT107I GGYRCC 1 cut(s) 872
BspTNI GGTCTC 1 cut(s) 476
BsrDI GCAATG 3 cut(s) 168, 580, 898
BsrFI RCCGGY 3 cut(s) 36, 107, 432
BsrI ACTGG 1 cut(s) 532
BssAI RCCGGY 3 cut(s) 36, 107, 432
BssECI CCNNGG 3 cut(s) 132, 811, 985
BssMI GATC 5 cut(s) 352, 445, 678, 807, 836
BssNAI GTATAC 1 cut(s) 453
BssT1I CCWWGG 2 cut(s) 811, 985
Bst1107I GTATAC 1 cut(s) 453
Bst2UI CCWGG 4 cut(s) 539, 683, 780, 870
Bst4CI ACNGT 1 cut(s) 695
BstAPI GCANNNNNTGC 2 cut(s) 167, 1088
BstC8I GCNNGC 8 cut(s) 109, 196, 412, 434, 464, 964, 1098, 1112
BstDEI CTNAG 4 cut(s) 380, 458, 543, 833
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 2 cut(s) 865, 1057
BstFNI CGCG 1 cut(s) 134
BstH2I RGCGCY 1 cut(s) 906
BstHHI GCGC 2 cut(s) 151, 905
BstKTI GATC 5 cut(s) 355, 448, 681, 810, 839
BstMAI GTCTC 2 cut(s) 419, 476
BstMBI GATC 5 cut(s) 352, 445, 678, 807, 836
BstNI CCWGG 4 cut(s) 539, 683, 780, 870
BstNSI RCATGY 1 cut(s) 1045
BstSCI CCNGG 4 cut(s) 537, 681, 778, 868
BstSFI CTRYAG 1 cut(s) 930
BstUI CGCG 1 cut(s) 134
BstV1I GCAGC 5 cut(s) 394, 416, 828, 944, 1060
BstV2I GAAGAC 1 cut(s) 995
BstX2I RGATCY 3 cut(s) 352, 678, 807
BstYI RGATCY 3 cut(s) 352, 678, 807
BstZ17I GTATAC 1 cut(s) 453
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 7 cut(s) 107, 267, 327, 432, 733, 773, 1110
BsuTUI ATCGAT 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 132
BtgZI GCGATG 1 cut(s) 205
BtsCI GGATG 2 cut(s) 865, 1057
BtsIMutI CAGTG 1 cut(s) 952
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 8 cut(s) 109, 196, 412, 434, 464, 964, 1098, 1112
CaiI CAGNNNCTG 4 cut(s) 407, 410, 538, 682
CfoI GCGC 2 cut(s) 151, 905
Cfr10I RCCGGY 3 cut(s) 36, 107, 432
Cfr13I GGNCC 5 cut(s) 266, 325, 731, 772, 1109
Cfr42I CCGCGG 1 cut(s) 135
ClaI ATCGAT 1 cut(s) 252
Csp6I GTAC 2 cut(s) 535, 886
CviAII CATG 4 cut(s) 112, 598, 1020, 1042
CviQI GTAC 2 cut(s) 535, 886
DdeI CTNAG 4 cut(s) 380, 458, 543, 833
DpnI GATC 5 cut(s) 354, 447, 680, 809, 838
DpnII GATC 5 cut(s) 352, 445, 678, 807, 836
EaeI YGGCCR 1 cut(s) 105
Eco130I CCWWGG 2 cut(s) 811, 985
Eco24I GRGCYC 1 cut(s) 834
Eco31I GGTCTC 1 cut(s) 476
Eco57I CTGAAG 2 cut(s) 384, 1089
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 4 cut(s) 537, 681, 778, 868
EcoT14I CCWWGG 2 cut(s) 811, 985
EcoT38I GRGCYC 1 cut(s) 834
ErhI CCWWGG 2 cut(s) 811, 985
FaeI CATG 4 cut(s) 115, 601, 1023, 1045
FaqI GGGAC 1 cut(s) 191
FatI CATG 4 cut(s) 111, 597, 1019, 1041
FauI CCCGC 1 cut(s) 1119
FblI GTMKAC 1 cut(s) 452
FokI GGATG 2 cut(s) 852, 1064
FriOI GRGCYC 1 cut(s) 834
FspBI CTAG 1 cut(s) 719
GlaI GCGC 2 cut(s) 150, 904
GsaI CCCAGC 2 cut(s) 326, 778
GsuI CTGGAG 1 cut(s) 324
HaeII RGCGCY 1 cut(s) 906
HaeIII GGCC 7 cut(s) 107, 267, 327, 432, 733, 773, 1110
HapII CCGG 5 cut(s) 37, 108, 158, 273, 433
HhaI GCGC 2 cut(s) 151, 905
Hin1II CATG 4 cut(s) 115, 601, 1023, 1045
Hin6I GCGC 2 cut(s) 149, 903
HinP1I GCGC 2 cut(s) 149, 903
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HinfI GANTC 5 cut(s) 125, 179, 279, 387, 493
HpaII CCGG 5 cut(s) 37, 108, 158, 273, 433
HphI GGTGA 3 cut(s) 274, 838, 923
Hpy166II GTNNAC 4 cut(s) 75, 453, 793, 886
Hpy188I TCNGA 5 cut(s) 65, 807, 836, 921, 1120
Hpy188III TCNNGA 1 cut(s) 481
Hpy8I GTNNAC 4 cut(s) 75, 453, 793, 886
HpyAV CCTTC 5 cut(s) 338, 612, 616, 662, 777
HpyCH4III ACNGT 1 cut(s) 695
HpyCH4V TGCA 6 cut(s) 350, 691, 744, 932, 1013, 1076
HpyF3I CTNAG 4 cut(s) 380, 458, 543, 833
Hsp92II CATG 4 cut(s) 115, 601, 1023, 1045
HspAI GCGC 2 cut(s) 149, 903
KroI GCCGGC 2 cut(s) 107, 432
KroNI GCCGGC 2 cut(s) 109, 434
KspI CCGCGG 1 cut(s) 135
Kzo9I GATC 5 cut(s) 352, 445, 678, 807, 836
LmnI GCTCC 3 cut(s) 221, 343, 570
Lsp1109I GCAGC 5 cut(s) 394, 416, 828, 944, 1060
LweI GCATC 4 cut(s) 181, 258, 770, 1042
MaeI CTAG 1 cut(s) 719
MaeIII GTNAC 3 cut(s) 844, 911, 1124
MalI GATC 5 cut(s) 354, 447, 680, 809, 838
MboI GATC 5 cut(s) 352, 445, 678, 807, 836
MboII GAAGA 4 cut(s) 25, 303, 606, 995
MflI RGATCY 3 cut(s) 352, 678, 807
MhlI GDGCHC 1 cut(s) 834
MluCI AATT 9 cut(s) 52, 78, 87, 232, 316, 391, 475, 726, 972
MlyI GAGTC 3 cut(s) 134, 173, 396
MnlI CCTC 7 cut(s) 257, 280, 605, 645, 699, 972, 1126
MroNI GCCGGC 2 cut(s) 107, 432
MseI TTAA 6 cut(s) 86, 90, 500, 660, 855, 1104
MslI CAYNNNNRTG 2 cut(s) 870, 881
MspA1I CMGCKG 2 cut(s) 134, 407
MspI CCGG 5 cut(s) 37, 108, 158, 273, 433
MspR9I CCNGG 4 cut(s) 539, 683, 780, 870
Mva1269I GAATGC 1 cut(s) 744
MvaI CCWGG 4 cut(s) 539, 683, 780, 870
MvnI CGCG 1 cut(s) 134
NaeI GCCGGC 2 cut(s) 109, 434
NdeII GATC 5 cut(s) 352, 445, 678, 807, 836
NgoMIV GCCGGC 2 cut(s) 107, 432
NlaIII CATG 4 cut(s) 115, 601, 1023, 1045
NlaIV GGNNCC 4 cut(s) 156, 268, 874, 983
NmuCI GTSAC 2 cut(s) 844, 911
NspI RCATGY 1 cut(s) 1045
PacI TTAATTAA 1 cut(s) 90
PciI ACATGT 1 cut(s) 1041
PctI GAATGC 1 cut(s) 744
PdiI GCCGGC 2 cut(s) 109, 434
PfeI GAWTC 2 cut(s) 279, 493
PflFI GACNNNGTC 1 cut(s) 797
PflMI CCANNNNNTGG 2 cut(s) 538, 1147
PleI GAGTC 3 cut(s) 133, 173, 395
PpsI GAGTC 3 cut(s) 133, 173, 395
PscI ACATGT 1 cut(s) 1041
PshBI ATTAAT 1 cut(s) 90
Psp6I CCWGG 4 cut(s) 537, 681, 778, 868
PspFI CCCAGC 2 cut(s) 322, 774
PspGI CCWGG 4 cut(s) 537, 681, 778, 868
PspN4I GGNNCC 4 cut(s) 156, 268, 874, 983
PspPI GGNCC 5 cut(s) 266, 325, 731, 772, 1109
PstI CTGCAG 1 cut(s) 934
PstNI CAGNNNCTG 4 cut(s) 407, 410, 538, 682
PsuI RGATCY 3 cut(s) 352, 678, 807
PsyI GACNNNGTC 1 cut(s) 797
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 2 cut(s) 536, 887
RsaNI GTAC 2 cut(s) 535, 886
RseI CAYNNNNRTG 2 cut(s) 870, 881
SacII CCGCGG 1 cut(s) 135
SaqAI TTAA 6 cut(s) 86, 90, 500, 660, 855, 1104
Sau3AI GATC 5 cut(s) 352, 445, 678, 807, 836
Sau96I GGNCC 5 cut(s) 266, 325, 731, 772, 1109
SchI GAGTC 3 cut(s) 134, 173, 396
ScrFI CCNGG 4 cut(s) 539, 683, 780, 870
SduI GDGCHC 1 cut(s) 834
SfaNI GCATC 4 cut(s) 181, 258, 770, 1042
SfcI CTRYAG 1 cut(s) 930
Sfr303I CCGCGG 1 cut(s) 135
SgrBI CCGCGG 1 cut(s) 135
SmiMI CAYNNNNRTG 2 cut(s) 870, 881
Sse9I AATT 9 cut(s) 52, 78, 87, 232, 316, 391, 475, 726, 972
SsiI CCGC 7 cut(s) 132, 134, 137, 140, 143, 146, 1112
SspMI CTAG 1 cut(s) 719
StyD4I CCNGG 4 cut(s) 537, 681, 778, 868
StyI CCWWGG 2 cut(s) 811, 985
TaaI ACNGT 1 cut(s) 695
TaqI TCGA 4 cut(s) 252, 420, 480, 548
TasI AATT 9 cut(s) 52, 78, 87, 232, 316, 391, 475, 726, 972
TauI GCSGC 6 cut(s) 134, 137, 140, 143, 146, 149
TfiI GAWTC 2 cut(s) 279, 493
Tru1I TTAA 6 cut(s) 86, 90, 500, 660, 855, 1104
Tru9I TTAA 6 cut(s) 86, 90, 500, 660, 855, 1104
TscAI CASTG 1 cut(s) 952
TseFI GTSAC 2 cut(s) 844, 911
TseI GCWGC 5 cut(s) 404, 407, 816, 932, 1073
Tsp45I GTSAC 2 cut(s) 844, 911
TspDTI ATGAA 1 cut(s) 199
TspRI CASTG 1 cut(s) 952
Tth111I GACNNNGTC 1 cut(s) 797
Van91I CCANNNNNTGG 2 cut(s) 538, 1147
VspI ATTAAT 1 cut(s) 90
XapI RAATTY 2 cut(s) 316, 475
XceI RCATGY 1 cut(s) 1045
XcmI CCANNNNNNNNNTGG 1 cut(s) 679
XmiI GTMKAC 1 cut(s) 452
XspI CTAG 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.