Rh6CG001500

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0016165
Physical Location & Seq
Reverse (-)
330012 .. 332080
2069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG001500.1

Sequence Viewer

Length: 963 bp
ATGCATGCATGCATGGGTGGTAAGAAGATGGCGGTAGCGATTTGTAATGACCGGCTGTGGTTAGCCAATTATACCATCGGAATGGTGTTTACAATGATCTTAATTAGTAGTAGTATGGTGGCCGGCATGGGATTTGGGGAGTCAGTGGCGGCGGCGGCGCAGGTTCCGGCAATGTTTGCGTTTGGGGACTCACTCATTGATGCTGGCAACAACAACTTCATAAGCTCCATCGCAAAATCCAATTATTATCCTTACGGCATCGATTTCAGGGGAGGCCCCTCCGGCAGATTCACCAATGGAAGAACTTTCGTTGATATGCTTGGAAATTTGCTGGGTATTCCTTATGCTCCAGCCTTTGCAGATCCTAATACAAAAGGAAACATAATGCTCAGGGGAGTCAATTATGCTTCAGCAGCTGCTGGCATACTCGATGAGACAGGCCGGCACTATGGAGATCGGTATACCCTAAGCCGGCAAGTGTTGAATTTCGAGACCACCTTGAATCAGTTAAGAACGATGATGGGGGGAACTAACCTGACCCAGTACCTGGCTAAGTCGATTGCCGTTTTGGTATTTGGGAGCAATGACTACATCAACAACTACCTCATGCCTTCCCTCTATTCTTCCAGCTACACTTACACTCCTCCTGCCTTTGCCAACCTTCTCCTTAATCGCTATGCCACACAGATTCTGTCTTTGCACGGTCTTGGATTGAGGAAGTTTGTGCTAGTAGGAATTGGGCCTTTGGGTTGCATTCCTAACCAAAGAGCATCAGTTAAGGCCCAGCCTGGAAGGTGTGTTGACAATGTCAATCAGATCCTTGGCAGCTTCAATCAAGGGCTCAGATCGCTCGTCACCCAACTTAACAACAACCATCCTGGTGCCATCTTTGTGTACGGCAATGCTTATGGCGCTTTTGGTGACATTCTGAATAACCCTGCACCCTATGGTAAGTATACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

320

Amino Acids

34.5

Weight (kDa)

9.28

Isoelectric Point (pI)

28.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 59 - 305 3.3e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71250
fragaria_vesca FvH4_2g00180
malus_domestica MD10G1000500.v1.1
prunus_persica Prupe.8G001600_v2.0.a1 Prupe.8G001600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249471
rosa_laevigata RLG00000015578
rosa_multiflora Rmu_ssc0000459.1_g000021
rosa_roxburghii Rroxscaffold_7G00217670
rosa_rugosa Rorug05G0492600
rosa_samantha Rh6AG002300 Rh6BG007800 Rh6CG001500 Rh6DG001500
rosa_wichuraiana Rw6G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 881
AccB7I CCANNNNNTGG 1 cut(s) 547
AccI GTMKAC 2 cut(s) 461, 956
AciI CCGC 4 cut(s) 32, 149, 152, 155
AclWI GGATC 2 cut(s) 356, 811
AcoI YGGCCR 1 cut(s) 120
AcsI RAATTY 2 cut(s) 325, 484
AcuI CTGAAG 1 cut(s) 393
AfaI GTAC 2 cut(s) 545, 896
AfiI CCNNNNNNNGG 2 cut(s) 471, 547
AgsI TTSAA 3 cut(s) 484, 502, 832
AjnI CCWGG 3 cut(s) 546, 787, 877
AluBI AGCT 4 cut(s) 225, 416, 630, 828
AluI AGCT 4 cut(s) 225, 416, 630, 828
Alw26I GTCTC 2 cut(s) 428, 485
AlwI GGATC 2 cut(s) 356, 811
AlwNI CAGNNNCTG 4 cut(s) 416, 419, 547, 691
AoxI GGCC 5 cut(s) 120, 274, 439, 740, 780
ApeKI GCWGC 3 cut(s) 413, 416, 825
ApoI RAATTY 2 cut(s) 325, 484
AspLEI GCGC 2 cut(s) 160, 914
AspS9I GGNCC 3 cut(s) 275, 740, 781
AsuHPI GGTGA 3 cut(s) 283, 847, 932
BanI GGYRCC 1 cut(s) 881
BanII GRGCYC 1 cut(s) 843
BbvI GCAGC 3 cut(s) 403, 425, 837
BccI CCATC 6 cut(s) 22, 83, 236, 514, 882, 893
BceAI ACGGC 3 cut(s) 271, 548, 913
BciT130I CCWGG 3 cut(s) 548, 789, 879
BcoDI GTCTC 2 cut(s) 428, 485
BfaI CTAG 1 cut(s) 728
BfoI RGCGCY 1 cut(s) 915
BfuAI ACCTGC 1 cut(s) 151
BglI GCCNNNNNGGC 1 cut(s) 282
BisI GCNGC 6 cut(s) 150, 153, 156, 414, 417, 826
BlsI GCNGC 6 cut(s) 151, 154, 157, 415, 418, 827
Bme1390I CCNGG 3 cut(s) 548, 789, 879
BmgT120I GGNCC 3 cut(s) 275, 740, 781
BmiI GGNNCC 3 cut(s) 165, 277, 883
BmrFI CCNGG 3 cut(s) 548, 789, 879
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 3 cut(s) 190, 267, 779
BmuI ACTGGG 1 cut(s) 535
BpmI CTGGAG 1 cut(s) 333
Bpu10I CCTNAGC 2 cut(s) 389, 467
Bsa29I ATCGAT 1 cut(s) 261
BsaI GGTCTC 1 cut(s) 485
BsaJI CCNNGG 1 cut(s) 820
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bsc4I CCNNNNNNNGG 2 cut(s) 471, 547
Bse118I RCCGGY 4 cut(s) 51, 122, 441, 471
Bse1I ACTGG 1 cut(s) 541
Bse3DI GCAATG 3 cut(s) 177, 589, 907
BseBI CCWGG 3 cut(s) 548, 789, 879
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 820
BseGI GGATG 1 cut(s) 874
BseLI CCNNNNNNNGG 2 cut(s) 471, 547
BseMI GCAATG 3 cut(s) 177, 589, 907
BseMII CTCAG 2 cut(s) 403, 856
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 1 cut(s) 633
BseXI GCAGC 3 cut(s) 403, 425, 837
BseYI CCCAGC 2 cut(s) 331, 783
BsgI GTGCAG 1 cut(s) 924
BshFI GGCC 5 cut(s) 122, 276, 441, 742, 782
BshNI GGYRCC 1 cut(s) 881
BshVI ATCGAT 1 cut(s) 261
BsiSI CCGG 6 cut(s) 52, 123, 167, 282, 442, 472
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 2 cut(s) 471, 547
BsmAI GTCTC 2 cut(s) 428, 485
BsmFI GGGAC 1 cut(s) 200
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 5 cut(s) 122, 276, 441, 742, 782
Bso31I GGTCTC 1 cut(s) 485
Bsp1286I GDGCHC 1 cut(s) 843
Bsp143I GATC 5 cut(s) 96, 361, 454, 816, 845
BspACI CCGC 4 cut(s) 32, 149, 152, 155
BspANI GGCC 5 cut(s) 122, 276, 441, 742, 782
BspCNI CTCAG 2 cut(s) 402, 855
BspDI ATCGAT 1 cut(s) 261
BspLI GGNNCC 3 cut(s) 165, 277, 883
BspMI ACCTGC 1 cut(s) 151
BspPI GGATC 2 cut(s) 356, 811
BspT107I GGYRCC 1 cut(s) 881
BspTNI GGTCTC 1 cut(s) 485
BsrDI GCAATG 3 cut(s) 177, 589, 907
BsrFI RCCGGY 4 cut(s) 51, 122, 441, 471
BsrI ACTGG 1 cut(s) 541
BssAI RCCGGY 4 cut(s) 51, 122, 441, 471
BssECI CCNNGG 1 cut(s) 820
BssMI GATC 5 cut(s) 96, 361, 454, 816, 845
BssNAI GTATAC 2 cut(s) 462, 957
BssT1I CCWWGG 1 cut(s) 820
Bst1107I GTATAC 2 cut(s) 462, 957
Bst2UI CCWGG 3 cut(s) 548, 789, 879
Bst4CI ACNGT 1 cut(s) 704
BstAPI GCANNNNNTGC 1 cut(s) 176
BstC8I GCNNGC 7 cut(s) 6, 10, 124, 205, 421, 443, 473
BstDEI CTNAG 4 cut(s) 389, 467, 552, 842
BstF5I GGATG 1 cut(s) 874
BstH2I RGCGCY 1 cut(s) 915
BstHHI GCGC 2 cut(s) 160, 914
BstKTI GATC 5 cut(s) 99, 364, 457, 819, 848
BstMAI GTCTC 2 cut(s) 428, 485
BstMBI GATC 5 cut(s) 96, 361, 454, 816, 845
BstMWI GCNNNNNNNGC 6 cut(s) 155, 176, 282, 413, 847, 911
BstNI CCWGG 3 cut(s) 548, 789, 879
BstNSI RCATGY 2 cut(s) 8, 12
BstSCI CCNGG 3 cut(s) 546, 787, 877
BstV1I GCAGC 3 cut(s) 403, 425, 837
BstX2I RGATCY 2 cut(s) 361, 816
BstXI CCANNNNNNTGG 1 cut(s) 82
BstYI RGATCY 2 cut(s) 361, 816
BstZ17I GTATAC 2 cut(s) 462, 957
Bsu15I ATCGAT 1 cut(s) 261
BsuRI GGCC 5 cut(s) 122, 276, 441, 742, 782
BsuTUI ATCGAT 1 cut(s) 261
BtgZI GCGATG 1 cut(s) 214
BtsCI GGATG 1 cut(s) 874
BtsIMutI CAGTG 1 cut(s) 150
BveI ACCTGC 1 cut(s) 151
Cac8I GCNNGC 7 cut(s) 6, 10, 124, 205, 421, 443, 473
CaiI CAGNNNCTG 4 cut(s) 416, 419, 547, 691
CfoI GCGC 2 cut(s) 160, 914
Cfr10I RCCGGY 4 cut(s) 51, 122, 441, 471
Cfr13I GGNCC 3 cut(s) 275, 740, 781
ClaI ATCGAT 1 cut(s) 261
Csp6I GTAC 2 cut(s) 544, 895
CviAII CATG 5 cut(s) 5, 9, 13, 127, 607
CviQI GTAC 2 cut(s) 544, 895
DdeI CTNAG 4 cut(s) 389, 467, 552, 842
DpnI GATC 5 cut(s) 98, 363, 456, 818, 847
DpnII GATC 5 cut(s) 96, 361, 454, 816, 845
EaeI YGGCCR 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 820
Eco24I GRGCYC 1 cut(s) 843
Eco31I GGTCTC 1 cut(s) 485
Eco57I CTGAAG 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 3 cut(s) 546, 787, 877
EcoT14I CCWWGG 1 cut(s) 820
EcoT22I ATGCAT 3 cut(s) 6, 10, 14
EcoT38I GRGCYC 1 cut(s) 843
ErhI CCWWGG 1 cut(s) 820
FaeI CATG 5 cut(s) 8, 12, 16, 130, 610
FaqI GGGAC 1 cut(s) 200
FatI CATG 5 cut(s) 4, 8, 12, 126, 606
FblI GTMKAC 2 cut(s) 461, 956
Fnu4HI GCNGC 6 cut(s) 150, 153, 156, 414, 417, 826
FokI GGATG 1 cut(s) 861
FriOI GRGCYC 1 cut(s) 843
Fsp4HI GCNGC 6 cut(s) 150, 153, 156, 414, 417, 826
FspBI CTAG 1 cut(s) 728
GlaI GCGC 2 cut(s) 159, 913
GluI GCNGC 6 cut(s) 150, 153, 156, 414, 417, 826
GsaI CCCAGC 2 cut(s) 335, 787
GsuI CTGGAG 1 cut(s) 333
HaeII RGCGCY 1 cut(s) 915
HaeIII GGCC 5 cut(s) 122, 276, 441, 742, 782
HapII CCGG 6 cut(s) 52, 123, 167, 282, 442, 472
HhaI GCGC 2 cut(s) 160, 914
Hin1II CATG 5 cut(s) 8, 12, 16, 130, 610
Hin6I GCGC 2 cut(s) 158, 912
HinP1I GCGC 2 cut(s) 158, 912
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HinfI GANTC 6 cut(s) 140, 188, 288, 396, 502, 688
HpaII CCGG 6 cut(s) 52, 123, 167, 282, 442, 472
HphI GGTGA 3 cut(s) 283, 847, 932
Hpy166II GTNNAC 5 cut(s) 90, 462, 802, 895, 957
Hpy188I TCNGA 4 cut(s) 80, 816, 845, 930
Hpy188III TCNNGA 1 cut(s) 490
Hpy8I GTNNAC 5 cut(s) 90, 462, 802, 895, 957
HpyAV CCTTC 3 cut(s) 621, 671, 786
HpyCH4III ACNGT 1 cut(s) 704
HpyCH4V TGCA 7 cut(s) 4, 8, 12, 359, 700, 753, 941
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 176, 282, 413, 847, 911
HpyF3I CTNAG 4 cut(s) 389, 467, 552, 842
Hsp92II CATG 5 cut(s) 8, 12, 16, 130, 610
HspAI GCGC 2 cut(s) 158, 912
KroI GCCGGC 3 cut(s) 122, 441, 471
KroNI GCCGGC 3 cut(s) 124, 443, 473
Kzo9I GATC 5 cut(s) 96, 361, 454, 816, 845
LmnI GCTCC 3 cut(s) 230, 352, 579
Lsp1109I GCAGC 3 cut(s) 403, 425, 837
LweI GCATC 3 cut(s) 190, 267, 779
MaeI CTAG 1 cut(s) 728
MaeIII GTNAC 2 cut(s) 853, 920
MalI GATC 5 cut(s) 98, 363, 456, 818, 847
MboI GATC 5 cut(s) 96, 361, 454, 816, 845
MboII GAAGA 3 cut(s) 37, 312, 615
MflI RGATCY 2 cut(s) 361, 816
MhlI GDGCHC 1 cut(s) 843
MluCI AATT 7 cut(s) 67, 102, 241, 325, 400, 484, 735
MlyI GAGTC 3 cut(s) 149, 182, 405
MnlI CCTC 6 cut(s) 266, 289, 614, 626, 654, 708
Mph1103I ATGCAT 3 cut(s) 6, 10, 14
MroNI GCCGGC 3 cut(s) 122, 441, 471
MseI TTAA 5 cut(s) 101, 509, 669, 777, 864
MslI CAYNNNNRTG 3 cut(s) 80, 879, 890
MspA1I CMGCKG 1 cut(s) 416
MspI CCGG 6 cut(s) 52, 123, 167, 282, 442, 472
MspR9I CCNGG 3 cut(s) 548, 789, 879
Mva1269I GAATGC 1 cut(s) 753
MvaI CCWGG 3 cut(s) 548, 789, 879
MwoI GCNNNNNNNGC 6 cut(s) 155, 176, 282, 413, 847, 911
NaeI GCCGGC 3 cut(s) 124, 443, 473
NdeII GATC 5 cut(s) 96, 361, 454, 816, 845
NgoMIV GCCGGC 3 cut(s) 122, 441, 471
NlaIII CATG 5 cut(s) 8, 12, 16, 130, 610
NlaIV GGNNCC 3 cut(s) 165, 277, 883
NmuCI GTSAC 2 cut(s) 853, 920
NsiI ATGCAT 3 cut(s) 6, 10, 14
NspI RCATGY 2 cut(s) 8, 12
PaeI GCATGC 2 cut(s) 8, 12
PctI GAATGC 1 cut(s) 753
PdiI GCCGGC 3 cut(s) 124, 443, 473
PfeI GAWTC 3 cut(s) 288, 502, 688
PflFI GACNNNGTC 1 cut(s) 806
PflMI CCANNNNNTGG 1 cut(s) 547
PkrI GCNGC 6 cut(s) 151, 154, 157, 415, 418, 827
PleI GAGTC 3 cut(s) 148, 182, 404
PpsI GAGTC 3 cut(s) 148, 182, 404
Psp6I CCWGG 3 cut(s) 546, 787, 877
PspFI CCCAGC 2 cut(s) 331, 783
PspGI CCWGG 3 cut(s) 546, 787, 877
PspN4I GGNNCC 3 cut(s) 165, 277, 883
PspPI GGNCC 3 cut(s) 275, 740, 781
PstNI CAGNNNCTG 4 cut(s) 416, 419, 547, 691
PsuI RGATCY 2 cut(s) 361, 816
PsyI GACNNNGTC 1 cut(s) 806
PvuII CAGCTG 1 cut(s) 416
RsaI GTAC 2 cut(s) 545, 896
RsaNI GTAC 2 cut(s) 544, 895
RseI CAYNNNNRTG 3 cut(s) 80, 879, 890
SaqAI TTAA 5 cut(s) 101, 509, 669, 777, 864
SatI GCNGC 6 cut(s) 150, 153, 156, 414, 417, 826
Sau3AI GATC 5 cut(s) 96, 361, 454, 816, 845
Sau96I GGNCC 3 cut(s) 275, 740, 781
SchI GAGTC 3 cut(s) 149, 182, 405
ScrFI CCNGG 3 cut(s) 548, 789, 879
SduI GDGCHC 1 cut(s) 843
SfaNI GCATC 3 cut(s) 190, 267, 779
SmiMI CAYNNNNRTG 3 cut(s) 80, 879, 890
SphI GCATGC 2 cut(s) 8, 12
Sse9I AATT 7 cut(s) 67, 102, 241, 325, 400, 484, 735
SsiI CCGC 4 cut(s) 32, 149, 152, 155
SspMI CTAG 1 cut(s) 728
StyD4I CCNGG 3 cut(s) 546, 787, 877
StyI CCWWGG 1 cut(s) 820
TaaI ACNGT 1 cut(s) 704
TaqI TCGA 4 cut(s) 261, 429, 489, 557
TasI AATT 7 cut(s) 67, 102, 241, 325, 400, 484, 735
TauI GCSGC 3 cut(s) 152, 155, 158
TfiI GAWTC 3 cut(s) 288, 502, 688
Tru1I TTAA 5 cut(s) 101, 509, 669, 777, 864
Tru9I TTAA 5 cut(s) 101, 509, 669, 777, 864
TscAI CASTG 1 cut(s) 150
TseFI GTSAC 2 cut(s) 853, 920
TseI GCWGC 3 cut(s) 413, 416, 825
Tsp45I GTSAC 2 cut(s) 853, 920
TspDTI ATGAA 1 cut(s) 208
TspRI CASTG 1 cut(s) 150
Tth111I GACNNNGTC 1 cut(s) 806
Van91I CCANNNNNTGG 1 cut(s) 547
XapI RAATTY 2 cut(s) 325, 484
XceI RCATGY 2 cut(s) 8, 12
XmiI GTMKAC 2 cut(s) 461, 956
XspI CTAG 1 cut(s) 728
Zsp2I ATGCAT 3 cut(s) 6, 10, 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.