Rw6G000290

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016165
Physical Location & Seq
Reverse (-)
580732 .. 582319
1588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000290.1

Sequence Viewer

Length: 1143 bp
ATGCATGCATGCATGGGTGGTAAGAAGATGGTAGCGATTTGTAATGACCGGCTGTGGTTAGCCAATTATACTATCGGAATGGTGTTTACAATTATCTTAATTAATAGTAGTATGGTGGCCGGCATGGGATTTGGGGAGTCAGCCGCCGCGGCGGCGGTGGCGCAGGTTCCGGCAATGTTTGCGTTTGGGGACTCACTCATTGATGCTGGCAACAACAACTTCATAAGCTCCATCGCAAAATCCAATTATTATCCTTACGGCATCGATTTCAGGGGAGGCCCCTCCGGCAGATTCACCAATGGAAGAACTTTCGTTGATATGCTTGGAAATTTGCTGGGCCTTCCTTATGCTCCAGCCTTTGCAGATCCTAATACAAAAGGAAACAGAATACTCAGGGGAGTCAATTATGCTTCAGCAGCTGCTGGCATACTCGATGAGACAGGCCGGCACTATGGAGATCGGTATACCCTAAGCCAGCAAGTGTTGAATTTCGAGACCACCTTGAATCAGTTAAGAACGATGATGGGGGGAACTAACCTGACCCAGTACCTGGCTAAGTCGATTGCCGTTTTGGTATTTGGGAGCAATGACTACATCAACAACTACCTCATGCCTTCCTTCTATTCTTCCAGCTACACTTACACTCCTCCTGCCTTTGCCAACCTTCTCCTTAATCGCTATGCCACACAGATCCTGGCTTTGCACGGAATTGGGCCTTTGGGTTGCATTCCTAACCAAAGAGCATCAGGTAAGGCCCAGCCTGGAAGGTGTGTTGACAATGTCAATGAGATCCTTGGCAGCTTCAATCAAGGGCTCAGATCGCTCGTCACCCAACTTAACAACAACCATCCTGGTGCCATCTTTGTGTACGGCAATGCTTATGGCGCTTTTGGTGACATTCTGAATAACCCTGCAGCCTATGGTTTCAGTGTGATTGATAGGGCTTGCTGTGGAATTGGGAGGAACCAAGGGCAGATAACTTGTCTTCCATTTGCAGTTCCATGCTCAAATAGAAATCAATACATGTTTTGGGATGCTTTCCACCCAACTGAAGCTGCAAACGCAGTCCTTGCTTGGCGAGCTTTTAATGGCCCGCCCTCTGATAGTTACCCCATCAATGTCCAACAAATGGCTCTCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

380

Amino Acids

41.03

Weight (kDa)

8.56

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 60 - 358 1.3e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71250
fragaria_vesca FvH4_2g00180
malus_domestica MD10G1000500.v1.1
prunus_persica Prupe.8G001600_v2.0.a1 Prupe.8G001600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249471
rosa_laevigata RLG00000015578
rosa_multiflora Rmu_ssc0000459.1_g000021
rosa_roxburghii Rroxscaffold_7G00217670
rosa_rugosa Rorug05G0492600
rosa_samantha Rh6AG002300 Rh6BG007800 Rh6CG001500 Rh6DG001500
rosa_wichuraiana Rw6G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 154
AccB1I GGYRCC 1 cut(s) 854
AccB7I CCANNNNNTGG 2 cut(s) 550, 1129
AccI GTMKAC 1 cut(s) 464
AccII CGCG 1 cut(s) 149
AciI CCGC 6 cut(s) 144, 147, 149, 152, 155, 1094
AclWI GGATC 3 cut(s) 359, 685, 784
AcoI YGGCCR 1 cut(s) 117
AcsI RAATTY 2 cut(s) 328, 487
AcuI CTGAAG 2 cut(s) 396, 1071
AfaI GTAC 2 cut(s) 548, 869
AfiI CCNNNNNNNGG 2 cut(s) 550, 1129
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 3 cut(s) 487, 505, 805
AjnI CCWGG 4 cut(s) 549, 693, 760, 850
AluBI AGCT 6 cut(s) 228, 419, 633, 801, 1055, 1082
AluI AGCT 6 cut(s) 228, 419, 633, 801, 1055, 1082
Alw26I GTCTC 2 cut(s) 431, 488
AlwI GGATC 3 cut(s) 359, 685, 784
AlwNI CAGNNNCTG 4 cut(s) 419, 422, 550, 694
AoxI GGCC 7 cut(s) 117, 277, 337, 442, 713, 753, 1090
ApeKI GCWGC 5 cut(s) 416, 419, 798, 914, 1055
ApoI RAATTY 2 cut(s) 328, 487
AseI ATTAAT 1 cut(s) 102
AspLEI GCGC 2 cut(s) 163, 887
AspS9I GGNCC 5 cut(s) 278, 337, 713, 754, 1091
AsuHPI GGTGA 3 cut(s) 286, 820, 905
BanI GGYRCC 1 cut(s) 854
BanII GRGCYC 1 cut(s) 816
BbsI GAAGAC 1 cut(s) 977
BbvI GCAGC 5 cut(s) 406, 428, 810, 926, 1042
BccI CCATC 6 cut(s) 22, 239, 517, 855, 866, 1121
BceAI ACGGC 3 cut(s) 274, 551, 886
BciT130I CCWGG 4 cut(s) 551, 695, 762, 852
BcoDI GTCTC 2 cut(s) 431, 488
BfmI CTRYAG 1 cut(s) 912
BfoI RGCGCY 1 cut(s) 888
BfuAI ACCTGC 1 cut(s) 154
BglI GCCNNNNNGGC 3 cut(s) 149, 152, 285
BisI GCNGC 9 cut(s) 144, 147, 150, 153, 417, 420, 799, 915, 1056
BlsI GCNGC 9 cut(s) 145, 148, 151, 154, 418, 421, 800, 916, 1057
Bme1390I CCNGG 4 cut(s) 551, 695, 762, 852
BmgT120I GGNCC 5 cut(s) 278, 337, 713, 754, 1091
BmiI GGNNCC 4 cut(s) 168, 280, 856, 965
BmrFI CCNGG 4 cut(s) 551, 695, 762, 852
BmrI ACTGGG 1 cut(s) 538
BmsI GCATC 4 cut(s) 193, 270, 752, 1024
BmuI ACTGGG 1 cut(s) 538
BpiI GAAGAC 1 cut(s) 977
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 470
Bsa29I ATCGAT 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 3 cut(s) 147, 793, 967
BsaXI ACNNNNNCTCC 2 cut(s) 628, 658
Bsc4I CCNNNNNNNGG 2 cut(s) 550, 1129
Bse118I RCCGGY 3 cut(s) 48, 119, 444
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 3 cut(s) 180, 592, 880
BseBI CCWGG 4 cut(s) 551, 695, 762, 852
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 3 cut(s) 147, 793, 967
BseGI GGATG 2 cut(s) 847, 1039
BseLI CCNNNNNNNGG 2 cut(s) 550, 1129
BseMI GCAATG 3 cut(s) 180, 592, 880
BseMII CTCAG 2 cut(s) 406, 829
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 1 cut(s) 636
BseXI GCAGC 5 cut(s) 406, 428, 810, 926, 1042
BseYI CCCAGC 2 cut(s) 334, 756
Bsh1236I CGCG 1 cut(s) 149
BshFI GGCC 7 cut(s) 119, 279, 339, 444, 715, 755, 1092
BshNI GGYRCC 1 cut(s) 854
BshVI ATCGAT 1 cut(s) 264
BsiSI CCGG 5 cut(s) 49, 120, 170, 285, 445
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 2 cut(s) 550, 1129
BsmAI GTCTC 2 cut(s) 431, 488
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 726
BsnI GGCC 7 cut(s) 119, 279, 339, 444, 715, 755, 1092
Bso31I GGTCTC 1 cut(s) 488
Bsp1286I GDGCHC 1 cut(s) 816
Bsp143I GATC 5 cut(s) 364, 457, 690, 789, 818
BspACI CCGC 6 cut(s) 144, 147, 149, 152, 155, 1094
BspANI GGCC 7 cut(s) 119, 279, 339, 444, 715, 755, 1092
BspCNI CTCAG 2 cut(s) 405, 828
BspDI ATCGAT 1 cut(s) 264
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 4 cut(s) 168, 280, 856, 965
BspMAI CTGCAG 1 cut(s) 916
BspMI ACCTGC 1 cut(s) 154
BspPI GGATC 3 cut(s) 359, 685, 784
BspT107I GGYRCC 1 cut(s) 854
BspTNI GGTCTC 1 cut(s) 488
BsrDI GCAATG 3 cut(s) 180, 592, 880
BsrFI RCCGGY 3 cut(s) 48, 119, 444
BsrI ACTGG 1 cut(s) 544
BssAI RCCGGY 3 cut(s) 48, 119, 444
BssECI CCNNGG 3 cut(s) 147, 793, 967
BssMI GATC 5 cut(s) 364, 457, 690, 789, 818
BssNAI GTATAC 1 cut(s) 465
BssT1I CCWWGG 2 cut(s) 793, 967
Bst1107I GTATAC 1 cut(s) 465
Bst2UI CCWGG 4 cut(s) 551, 695, 762, 852
BstAPI GCANNNNNTGC 2 cut(s) 179, 1070
BstDEI CTNAG 4 cut(s) 392, 470, 555, 815
BstDSI CCRYGG 1 cut(s) 147
BstF5I GGATG 2 cut(s) 847, 1039
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 1 cut(s) 888
BstHHI GCGC 2 cut(s) 163, 887
BstKTI GATC 5 cut(s) 367, 460, 693, 792, 821
BstMAI GTCTC 2 cut(s) 431, 488
BstMBI GATC 5 cut(s) 364, 457, 690, 789, 818
BstNI CCWGG 4 cut(s) 551, 695, 762, 852
BstNSI RCATGY 3 cut(s) 8, 12, 1027
BstSCI CCNGG 4 cut(s) 549, 693, 760, 850
BstSFI CTRYAG 1 cut(s) 912
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 5 cut(s) 406, 428, 810, 926, 1042
BstV2I GAAGAC 1 cut(s) 977
BstX2I RGATCY 3 cut(s) 364, 690, 789
BstYI RGATCY 3 cut(s) 364, 690, 789
BstZ17I GTATAC 1 cut(s) 465
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 7 cut(s) 119, 279, 339, 444, 715, 755, 1092
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 2 cut(s) 847, 1039
BtsIMutI CAGTG 1 cut(s) 934
BveI ACCTGC 1 cut(s) 154
CaiI CAGNNNCTG 4 cut(s) 419, 422, 550, 694
CfoI GCGC 2 cut(s) 163, 887
Cfr10I RCCGGY 3 cut(s) 48, 119, 444
Cfr13I GGNCC 5 cut(s) 278, 337, 713, 754, 1091
Cfr42I CCGCGG 1 cut(s) 150
ClaI ATCGAT 1 cut(s) 264
Csp6I GTAC 2 cut(s) 547, 868
CviAII CATG 7 cut(s) 5, 9, 13, 124, 610, 1002, 1024
CviQI GTAC 2 cut(s) 547, 868
DdeI CTNAG 4 cut(s) 392, 470, 555, 815
DpnI GATC 5 cut(s) 366, 459, 692, 791, 820
DpnII GATC 5 cut(s) 364, 457, 690, 789, 818
EaeI YGGCCR 1 cut(s) 117
Eco130I CCWWGG 2 cut(s) 793, 967
Eco24I GRGCYC 1 cut(s) 816
Eco31I GGTCTC 1 cut(s) 488
Eco57I CTGAAG 2 cut(s) 396, 1071
EcoO109I RGGNCCY 1 cut(s) 278
EcoRII CCWGG 4 cut(s) 549, 693, 760, 850
EcoT14I CCWWGG 2 cut(s) 793, 967
EcoT22I ATGCAT 3 cut(s) 6, 10, 14
EcoT38I GRGCYC 1 cut(s) 816
ErhI CCWWGG 2 cut(s) 793, 967
FaeI CATG 7 cut(s) 8, 12, 16, 127, 613, 1005, 1027
FaqI GGGAC 1 cut(s) 203
FatI CATG 7 cut(s) 4, 8, 12, 123, 609, 1001, 1023
FauI CCCGC 1 cut(s) 1101
FblI GTMKAC 1 cut(s) 464
Fnu4HI GCNGC 9 cut(s) 144, 147, 150, 153, 417, 420, 799, 915, 1056
FokI GGATG 2 cut(s) 834, 1046
FriOI GRGCYC 1 cut(s) 816
Fsp4HI GCNGC 9 cut(s) 144, 147, 150, 153, 417, 420, 799, 915, 1056
GlaI GCGC 2 cut(s) 162, 886
GluI GCNGC 9 cut(s) 144, 147, 150, 153, 417, 420, 799, 915, 1056
GsaI CCCAGC 2 cut(s) 338, 760
GsuI CTGGAG 1 cut(s) 336
HaeII RGCGCY 1 cut(s) 888
HaeIII GGCC 7 cut(s) 119, 279, 339, 444, 715, 755, 1092
HapII CCGG 5 cut(s) 49, 120, 170, 285, 445
HhaI GCGC 2 cut(s) 163, 887
Hin1II CATG 7 cut(s) 8, 12, 16, 127, 613, 1005, 1027
Hin6I GCGC 2 cut(s) 161, 885
HinP1I GCGC 2 cut(s) 161, 885
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 5 cut(s) 137, 191, 291, 399, 505
HpaII CCGG 5 cut(s) 49, 120, 170, 285, 445
HphI GGTGA 3 cut(s) 286, 820, 905
Hpy166II GTNNAC 4 cut(s) 87, 465, 775, 868
Hpy188I TCNGA 4 cut(s) 77, 818, 903, 1102
Hpy188III TCNNGA 1 cut(s) 493
Hpy8I GTNNAC 4 cut(s) 87, 465, 775, 868
HpyAV CCTTC 5 cut(s) 350, 624, 628, 674, 759
HpyCH4V TGCA 9 cut(s) 4, 8, 12, 362, 703, 726, 914, 995, 1058
HpyF3I CTNAG 4 cut(s) 392, 470, 555, 815
Hsp92II CATG 7 cut(s) 8, 12, 16, 127, 613, 1005, 1027
HspAI GCGC 2 cut(s) 161, 885
KroI GCCGGC 2 cut(s) 119, 444
KroNI GCCGGC 2 cut(s) 121, 446
KspI CCGCGG 1 cut(s) 150
Kzo9I GATC 5 cut(s) 364, 457, 690, 789, 818
LmnI GCTCC 3 cut(s) 233, 355, 582
Lsp1109I GCAGC 5 cut(s) 406, 428, 810, 926, 1042
LweI GCATC 4 cut(s) 193, 270, 752, 1024
MaeIII GTNAC 3 cut(s) 826, 893, 1106
MalI GATC 5 cut(s) 366, 459, 692, 791, 820
MboI GATC 5 cut(s) 364, 457, 690, 789, 818
MboII GAAGA 4 cut(s) 37, 315, 618, 977
MflI RGATCY 3 cut(s) 364, 690, 789
MhlI GDGCHC 1 cut(s) 816
MluCI AATT 9 cut(s) 64, 90, 99, 244, 328, 403, 487, 708, 954
MlyI GAGTC 3 cut(s) 146, 185, 408
MnlI CCTC 6 cut(s) 269, 292, 617, 657, 954, 1108
Mph1103I ATGCAT 3 cut(s) 6, 10, 14
MroNI GCCGGC 2 cut(s) 119, 444
MseI TTAA 6 cut(s) 98, 102, 512, 672, 837, 1086
MslI CAYNNNNRTG 2 cut(s) 852, 863
MspA1I CMGCKG 2 cut(s) 149, 419
MspI CCGG 5 cut(s) 49, 120, 170, 285, 445
MspR9I CCNGG 4 cut(s) 551, 695, 762, 852
Mva1269I GAATGC 1 cut(s) 726
MvaI CCWGG 4 cut(s) 551, 695, 762, 852
MvnI CGCG 1 cut(s) 149
NaeI GCCGGC 2 cut(s) 121, 446
NdeII GATC 5 cut(s) 364, 457, 690, 789, 818
NgoMIV GCCGGC 2 cut(s) 119, 444
NlaIII CATG 7 cut(s) 8, 12, 16, 127, 613, 1005, 1027
NlaIV GGNNCC 4 cut(s) 168, 280, 856, 965
NmuCI GTSAC 2 cut(s) 826, 893
NsiI ATGCAT 3 cut(s) 6, 10, 14
NspI RCATGY 3 cut(s) 8, 12, 1027
PacI TTAATTAA 1 cut(s) 102
PaeI GCATGC 2 cut(s) 8, 12
PciI ACATGT 1 cut(s) 1023
PctI GAATGC 1 cut(s) 726
PdiI GCCGGC 2 cut(s) 121, 446
PfeI GAWTC 2 cut(s) 291, 505
PflFI GACNNNGTC 1 cut(s) 779
PflMI CCANNNNNTGG 2 cut(s) 550, 1129
PkrI GCNGC 9 cut(s) 145, 148, 151, 154, 418, 421, 800, 916, 1057
PleI GAGTC 3 cut(s) 145, 185, 407
PpsI GAGTC 3 cut(s) 145, 185, 407
PscI ACATGT 1 cut(s) 1023
PshBI ATTAAT 1 cut(s) 102
Psp6I CCWGG 4 cut(s) 549, 693, 760, 850
PspFI CCCAGC 2 cut(s) 334, 756
PspGI CCWGG 4 cut(s) 549, 693, 760, 850
PspN4I GGNNCC 4 cut(s) 168, 280, 856, 965
PspPI GGNCC 5 cut(s) 278, 337, 713, 754, 1091
PstI CTGCAG 1 cut(s) 916
PstNI CAGNNNCTG 4 cut(s) 419, 422, 550, 694
PsuI RGATCY 3 cut(s) 364, 690, 789
PsyI GACNNNGTC 1 cut(s) 779
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 2 cut(s) 548, 869
RsaNI GTAC 2 cut(s) 547, 868
RseI CAYNNNNRTG 2 cut(s) 852, 863
SacII CCGCGG 1 cut(s) 150
SaqAI TTAA 6 cut(s) 98, 102, 512, 672, 837, 1086
SatI GCNGC 9 cut(s) 144, 147, 150, 153, 417, 420, 799, 915, 1056
Sau3AI GATC 5 cut(s) 364, 457, 690, 789, 818
Sau96I GGNCC 5 cut(s) 278, 337, 713, 754, 1091
SchI GAGTC 3 cut(s) 146, 185, 408
ScrFI CCNGG 4 cut(s) 551, 695, 762, 852
SduI GDGCHC 1 cut(s) 816
SfaNI GCATC 4 cut(s) 193, 270, 752, 1024
SfcI CTRYAG 1 cut(s) 912
Sfr303I CCGCGG 1 cut(s) 150
SgrBI CCGCGG 1 cut(s) 150
SmiMI CAYNNNNRTG 2 cut(s) 852, 863
SphI GCATGC 2 cut(s) 8, 12
Sse9I AATT 9 cut(s) 64, 90, 99, 244, 328, 403, 487, 708, 954
SsiI CCGC 6 cut(s) 144, 147, 149, 152, 155, 1094
StyD4I CCNGG 4 cut(s) 549, 693, 760, 850
StyI CCWWGG 2 cut(s) 793, 967
TaqI TCGA 4 cut(s) 264, 432, 492, 560
TasI AATT 9 cut(s) 64, 90, 99, 244, 328, 403, 487, 708, 954
TauI GCSGC 4 cut(s) 146, 149, 152, 155
TfiI GAWTC 2 cut(s) 291, 505
Tru1I TTAA 6 cut(s) 98, 102, 512, 672, 837, 1086
Tru9I TTAA 6 cut(s) 98, 102, 512, 672, 837, 1086
TscAI CASTG 1 cut(s) 934
TseFI GTSAC 2 cut(s) 826, 893
TseI GCWGC 5 cut(s) 416, 419, 798, 914, 1055
Tsp45I GTSAC 2 cut(s) 826, 893
TspDTI ATGAA 1 cut(s) 211
TspGWI ACGGA 1 cut(s) 720
TspRI CASTG 1 cut(s) 934
Tth111I GACNNNGTC 1 cut(s) 779
Van91I CCANNNNNTGG 2 cut(s) 550, 1129
VspI ATTAAT 1 cut(s) 102
XapI RAATTY 2 cut(s) 328, 487
XceI RCATGY 3 cut(s) 8, 12, 1027
XcmI CCANNNNNNNNNTGG 1 cut(s) 691
XmiI GTMKAC 1 cut(s) 464
Zsp2I ATGCAT 3 cut(s) 6, 10, 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.