Prupe.8G001600_v2.0.a1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0016165
Physical Location & Seq
Reverse (-)
137259 .. 138869
1611 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G001600.2

Sequence Viewer

Length: 1134 bp
ATGGGTGGTAAGAAGAGGATTTGTGATGAAGGGGAAGGGGTGTGGTTGGGCAGTAGTGGGGTGGTGCTGATCATGATGATTTTAGTGGTGCAGTGCTCAACAAGTATTGGGTCAGTGTCAGCAGCAGCGCAGGTTCCAGCAATGTTTATTTTTGGGGACTCATTGATTGATGTTGGCAACAACAACTACCTTAGCTCCCTTGCAAAATCCAATTATTATCCTTATGGCTGCGATTTCAGGGCAGGCCCTACTGGCAGATTCTCTAATGGAAGAACTGTTGTTGATATGCTTGGAAATTTGCTGGGTCTTCCATATGTTCCACCCTTTGCAGATCCTAATACAAAAGGAACCAAAATACTTGGGGGAGTAAATTATGCTTCCGCAGCTGCTGGCATACTTGATGAGACAGGCCAACACTTTGGACAGCGGTATAGCCTTAGCCAGCAAGTGCTGAATTTTGAGAGCACCTTGGATCAGTTAAGACCGATGATGAGTGGAGCCAACATGACCCAGTACTTGGCTAAGTCCATAGCAGTTTTGGAGTTTGGAAGCAATGACTACATCAACAACTACCTCTTGCCTTCCCTCTATTCTTCCAGCTACATTTACACTCCTCCTGCCTTTGCCAACCTTCTCCTTAATCGCTATACTCAACAGATTCTGGCGTTGCATAGCGTTGGATTAAAGAAGTTTGTGCTAGCAGGAATTGGGCCATTGGGGTGCATCCCTAACCAAAGAGCCTTAGCCCAGCCCGGAAGGTGTGTGGATTATGTGAATCAGATCCTTGGTAGCTTCAATGAAGGGCTTAGATCACTGGCCAACCAACTCAACACCAACCATCCTGGAGCCATCTTTGTCTATGCCAATACTTATGCTGCCTTTGGTGACATGCTTAACAATCCTGCCCTTTATGGGTTCAGTGTGATTGATAGAGGGTGCTGTGGAATTGGGAGGGACAGAGGGCAGATAACATGTCTTCCATATTCGGTTCCATGCGCAAATAGAAATCAGTACATTTTTTGGGATGCCTACCACCCAACTGAAGCTGCAAATGCCGTCCTTGCTTGGCGGGCTTTCAACGGCCCACCCTCTGATTGCTATCCAATCAACGTCCAACAAATGGCTCTCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

378

Amino Acids

40.78

Weight (kDa)

7.48

Isoelectric Point (pI)

24.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71250
fragaria_vesca FvH4_2g00180
malus_domestica MD10G1000500.v1.1
prunus_persica Prupe.8G001600_v2.0.a1 Prupe.8G001600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249471
rosa_laevigata RLG00000015578
rosa_multiflora Rmu_ssc0000459.1_g000021
rosa_roxburghii Rroxscaffold_7G00217670
rosa_rugosa Rorug05G0492600
rosa_samantha Rh6AG002300 Rh6BG007800 Rh6CG001500 Rh6DG001500
rosa_wichuraiana Rw6G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 997
Acc36I ACCTGC 1 cut(s) 121
AccB7I CCANNNNNTGG 2 cut(s) 517, 1120
AciI CCGC 3 cut(s) 381, 427, 1069
AclWI GGATC 3 cut(s) 326, 480, 775
AcoI YGGCCR 1 cut(s) 816
AcsI RAATTY 2 cut(s) 295, 454
AcuI CTGAAG 1 cut(s) 1062
AfaI GTAC 2 cut(s) 515, 1013
AfiI CCNNNNNNNGG 3 cut(s) 517, 912, 1120
AflIII ACRYGT 1 cut(s) 971
AgsI TTSAA 2 cut(s) 796, 1078
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 5 cut(s) 195, 386, 600, 792, 1046
AluI AGCT 5 cut(s) 195, 386, 600, 792, 1046
Alw21I GWGCWC 2 cut(s) 98, 467
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 3 cut(s) 326, 480, 775
AlwNI CAGNNNCTG 2 cut(s) 389, 661
AoxI GGCC 5 cut(s) 244, 409, 710, 816, 1081
ApeKI GCWGC 7 cut(s) 122, 125, 228, 383, 386, 875, 1046
ApoI RAATTY 2 cut(s) 295, 454
AspLEI GCGC 2 cut(s) 130, 998
AspS9I GGNCC 3 cut(s) 245, 710, 1082
AsuC2I CCSGG 1 cut(s) 753
AsuHPI GGTGA 1 cut(s) 896
AsuNHI GCTAGC 1 cut(s) 697
BalI TGGCCA 1 cut(s) 818
BbsI GAAGAC 2 cut(s) 299, 968
Bbv12I GWGCWC 2 cut(s) 98, 467
BbvI GCAGC 7 cut(s) 134, 137, 215, 373, 395, 862, 1033
BccI CCATC 2 cut(s) 846, 857
BceAI ACGGC 2 cut(s) 1040, 1096
BciT130I CCWGG 1 cut(s) 843
BclI TGATCA 1 cut(s) 69
BcnI CCSGG 1 cut(s) 753
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 1 cut(s) 698
BfuAI ACCTGC 1 cut(s) 121
BglI GCCNNNNNGGC 1 cut(s) 252
BisI GCNGC 7 cut(s) 123, 126, 229, 384, 387, 876, 1047
BlsI GCNGC 7 cut(s) 124, 127, 230, 385, 388, 877, 1048
BmcAI AGTACT 1 cut(s) 515
Bme1390I CCNGG 2 cut(s) 753, 843
BmgT120I GGNCC 3 cut(s) 245, 710, 1082
BmiI GGNNCC 5 cut(s) 135, 349, 499, 847, 990
BmrFI CCNGG 2 cut(s) 753, 843
BmrI ACTGGG 1 cut(s) 505
BmsI GCATC 2 cut(s) 732, 1015
BmtI GCTAGC 1 cut(s) 701
BmuI ACTGGG 1 cut(s) 505
BpiI GAAGAC 2 cut(s) 299, 968
BpmI CTGGAG 1 cut(s) 864
Bpu10I CCTNAGC 3 cut(s) 191, 437, 742
BpuMI CCSGG 1 cut(s) 753
BsaBI GATNNNNATC 1 cut(s) 1098
BsaJI CCNNGG 2 cut(s) 468, 784
BsaXI ACNNNNNCTCC 2 cut(s) 179, 209
Bsc4I CCNNNNNNNGG 3 cut(s) 517, 912, 1120
Bse1I ACTGG 3 cut(s) 256, 511, 819
Bse3DI GCAATG 2 cut(s) 147, 559
Bse8I GATNNNNATC 1 cut(s) 1098
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 2 cut(s) 468, 784
BseGI GGATG 3 cut(s) 723, 838, 1030
BseJI GATNNNNATC 1 cut(s) 1098
BseLI CCNNNNNNNGG 3 cut(s) 517, 912, 1120
BseMI GCAATG 2 cut(s) 147, 559
BseNI ACTGG 3 cut(s) 256, 511, 819
BseRI GAGGAG 1 cut(s) 603
BseXI GCAGC 7 cut(s) 134, 137, 215, 373, 395, 862, 1033
BseYI CCCAGC 2 cut(s) 301, 747
BsgI GTGCAG 1 cut(s) 110
BshFI GGCC 5 cut(s) 246, 411, 712, 818, 1083
BsiHKAI GWGCWC 2 cut(s) 98, 467
BsiSI CCGG 1 cut(s) 753
BslFI GGGAC 2 cut(s) 170, 968
BslI CCNNNNNNNGG 3 cut(s) 517, 912, 1120
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 2 cut(s) 170, 968
BsnI GGCC 5 cut(s) 246, 411, 712, 818, 1083
Bsp1286I GDGCHC 2 cut(s) 98, 467
Bsp143I GATC 5 cut(s) 69, 331, 472, 780, 809
BspACI CCGC 3 cut(s) 381, 427, 1069
BspANI GGCC 5 cut(s) 246, 411, 712, 818, 1083
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 5 cut(s) 135, 349, 499, 847, 990
BspMI ACCTGC 1 cut(s) 121
BspOI GCTAGC 1 cut(s) 701
BspPI GGATC 3 cut(s) 326, 480, 775
BsrDI GCAATG 2 cut(s) 147, 559
BsrI ACTGG 3 cut(s) 256, 511, 819
BssECI CCNNGG 2 cut(s) 468, 784
BssMI GATC 5 cut(s) 69, 331, 472, 780, 809
BssT1I CCWWGG 2 cut(s) 468, 784
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 1 cut(s) 8
BstC8I GCNNGC 5 cut(s) 244, 391, 443, 699, 1071
BstDEI CTNAG 5 cut(s) 191, 437, 522, 742, 806
BstF5I GGATG 3 cut(s) 723, 838, 1030
BstHHI GCGC 2 cut(s) 130, 998
BstKTI GATC 5 cut(s) 72, 334, 475, 783, 812
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 5 cut(s) 69, 331, 472, 780, 809
BstMWI GCNNNNNNNGC 5 cut(s) 252, 383, 1052, 1061, 1070
BstNI CCWGG 1 cut(s) 843
BstNSI RCATGY 2 cut(s) 892, 975
BstSCI CCNGG 2 cut(s) 751, 841
BstV1I GCAGC 7 cut(s) 134, 137, 215, 373, 395, 862, 1033
BstV2I GAAGAC 2 cut(s) 299, 968
BstX2I RGATCY 2 cut(s) 331, 780
BstXI CCANNNNNNTGG 1 cut(s) 419
BstYI RGATCY 2 cut(s) 331, 780
BsuRI GGCC 5 cut(s) 246, 411, 712, 818, 1083
BtsCI GGATG 3 cut(s) 723, 838, 1030
BtsI GCAGTG 1 cut(s) 98
BtsIMutI CAGTG 4 cut(s) 98, 120, 812, 925
BveI ACCTGC 1 cut(s) 121
Cac8I GCNNGC 5 cut(s) 244, 391, 443, 699, 1071
CaiI CAGNNNCTG 2 cut(s) 389, 661
CciI TCATGA 1 cut(s) 72
CfoI GCGC 2 cut(s) 130, 998
Cfr13I GGNCC 3 cut(s) 245, 710, 1082
Csp6I GTAC 2 cut(s) 514, 1012
CviAII CATG 5 cut(s) 73, 505, 889, 972, 993
CviQI GTAC 2 cut(s) 514, 1012
DdeI CTNAG 5 cut(s) 191, 437, 522, 742, 806
DpnI GATC 5 cut(s) 71, 333, 474, 782, 811
DpnII GATC 5 cut(s) 69, 331, 472, 780, 809
EaeI YGGCCR 1 cut(s) 816
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
Eco130I CCWWGG 2 cut(s) 468, 784
Eco57I CTGAAG 1 cut(s) 1062
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 2 cut(s) 468, 784
ErhI CCWWGG 2 cut(s) 468, 784
FaeI CATG 5 cut(s) 76, 508, 892, 975, 996
FaqI GGGAC 2 cut(s) 170, 968
FatI CATG 5 cut(s) 72, 504, 888, 971, 992
FauI CCCGC 1 cut(s) 1062
FauNDI CATATG 1 cut(s) 313
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 7 cut(s) 123, 126, 229, 384, 387, 876, 1047
FokI GGATG 3 cut(s) 710, 825, 1037
Fsp4HI GCNGC 7 cut(s) 123, 126, 229, 384, 387, 876, 1047
FspBI CTAG 1 cut(s) 698
FspI TGCGCA 1 cut(s) 997
GlaI GCGC 2 cut(s) 129, 997
GluI GCNGC 7 cut(s) 123, 126, 229, 384, 387, 876, 1047
GsaI CCCAGC 2 cut(s) 305, 751
GsuI CTGGAG 1 cut(s) 864
HaeIII GGCC 5 cut(s) 246, 411, 712, 818, 1083
HapII CCGG 1 cut(s) 753
HhaI GCGC 2 cut(s) 130, 998
Hin1II CATG 5 cut(s) 76, 508, 892, 975, 996
Hin6I GCGC 2 cut(s) 128, 996
HinP1I GCGC 2 cut(s) 128, 996
HinfI GANTC 4 cut(s) 158, 258, 658, 775
HpaII CCGG 1 cut(s) 753
HphI GGTGA 1 cut(s) 896
Hpy188I TCNGA 3 cut(s) 780, 1093, 1133
Hpy188III TCNNGA 1 cut(s) 73
HpyAV CCTTC 6 cut(s) 23, 29, 591, 641, 750, 794
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 1 cut(s) 1110
HpyCH4V TGCA 6 cut(s) 91, 203, 329, 670, 723, 1049
HpyF10VI GCNNNNNNNGC 5 cut(s) 252, 383, 1052, 1061, 1070
HpyF3I CTNAG 5 cut(s) 191, 437, 522, 742, 806
HpySE526I ACGT 1 cut(s) 1110
Hsp92II CATG 5 cut(s) 76, 508, 892, 975, 996
HspAI GCGC 2 cut(s) 128, 996
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 5 cut(s) 69, 331, 472, 780, 809
LmnI GCTCC 3 cut(s) 200, 497, 845
Lsp1109I GCAGC 7 cut(s) 134, 137, 215, 373, 395, 862, 1033
LweI GCATC 2 cut(s) 732, 1015
MaeI CTAG 1 cut(s) 698
MaeII ACGT 1 cut(s) 1110
MaeIII GTNAC 1 cut(s) 884
MalI GATC 5 cut(s) 71, 333, 474, 782, 811
MboI GATC 5 cut(s) 69, 331, 472, 780, 809
MboII GAAGA 5 cut(s) 25, 282, 299, 585, 968
MflI RGATCY 2 cut(s) 331, 780
MhlI GDGCHC 2 cut(s) 98, 467
MlsI TGGCCA 1 cut(s) 818
MluCI AATT 6 cut(s) 211, 295, 370, 454, 705, 945
MluNI TGGCCA 1 cut(s) 818
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 658
MnlI CCTC 8 cut(s) 9, 584, 596, 624, 926, 945, 953, 1099
Mox20I TGGCCA 1 cut(s) 818
MscI TGGCCA 1 cut(s) 818
MseI TTAA 4 cut(s) 479, 639, 683, 894
MslI CAYNNNNRTG 1 cut(s) 718
Msp20I TGGCCA 1 cut(s) 818
MspA1I CMGCKG 2 cut(s) 386, 427
MspI CCGG 1 cut(s) 753
MspR9I CCNGG 2 cut(s) 753, 843
MvaI CCWGG 1 cut(s) 843
MwoI GCNNNNNNNGC 5 cut(s) 252, 383, 1052, 1061, 1070
NciI CCSGG 1 cut(s) 753
NdeI CATATG 1 cut(s) 313
NdeII GATC 5 cut(s) 69, 331, 472, 780, 809
NheI GCTAGC 1 cut(s) 697
NlaIII CATG 5 cut(s) 76, 508, 892, 975, 996
NlaIV GGNNCC 5 cut(s) 135, 349, 499, 847, 990
NmuCI GTSAC 1 cut(s) 884
NsbI TGCGCA 1 cut(s) 997
NspI RCATGY 2 cut(s) 892, 975
PagI TCATGA 1 cut(s) 72
PciI ACATGT 1 cut(s) 971
PfeI GAWTC 3 cut(s) 258, 658, 775
PflMI CCANNNNNTGG 2 cut(s) 517, 1120
PfoI TCCNGGA 1 cut(s) 841
PkrI GCNGC 7 cut(s) 124, 127, 230, 385, 388, 877, 1048
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PscI ACATGT 1 cut(s) 971
Psp6I CCWGG 1 cut(s) 841
PspFI CCCAGC 2 cut(s) 301, 747
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 5 cut(s) 135, 349, 499, 847, 990
PspPI GGNCC 3 cut(s) 245, 710, 1082
PstNI CAGNNNCTG 2 cut(s) 389, 661
PsuI RGATCY 2 cut(s) 331, 780
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 515, 1013
RsaNI GTAC 2 cut(s) 514, 1012
RseI CAYNNNNRTG 1 cut(s) 718
SaqAI TTAA 4 cut(s) 479, 639, 683, 894
SatI GCNGC 7 cut(s) 123, 126, 229, 384, 387, 876, 1047
Sau3AI GATC 5 cut(s) 69, 331, 472, 780, 809
Sau96I GGNCC 3 cut(s) 245, 710, 1082
ScaI AGTACT 1 cut(s) 515
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 2 cut(s) 753, 843
SduI GDGCHC 2 cut(s) 98, 467
SfaNI GCATC 2 cut(s) 732, 1015
SmiMI CAYNNNNRTG 1 cut(s) 718
Sse9I AATT 6 cut(s) 211, 295, 370, 454, 705, 945
SsiI CCGC 3 cut(s) 381, 427, 1069
SspMI CTAG 1 cut(s) 698
StyD4I CCNGG 2 cut(s) 751, 841
StyI CCWWGG 2 cut(s) 468, 784
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 1 cut(s) 1113
TaqII GACCGA 1 cut(s) 499
TasI AATT 6 cut(s) 211, 295, 370, 454, 705, 945
TatI WGTACW 2 cut(s) 513, 1011
TfiI GAWTC 3 cut(s) 258, 658, 775
Tru1I TTAA 4 cut(s) 479, 639, 683, 894
Tru9I TTAA 4 cut(s) 479, 639, 683, 894
TscAI CASTG 4 cut(s) 98, 120, 819, 925
TseFI GTSAC 1 cut(s) 884
TseI GCWGC 7 cut(s) 122, 125, 228, 383, 386, 875, 1046
Tsp45I GTSAC 1 cut(s) 884
TspDTI ATGAA 2 cut(s) 42, 813
TspRI CASTG 4 cut(s) 98, 120, 819, 925
Van91I CCANNNNNTGG 2 cut(s) 517, 1120
XapI RAATTY 2 cut(s) 295, 454
XceI RCATGY 2 cut(s) 892, 975
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XspI CTAG 1 cut(s) 698
ZrmI AGTACT 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.