MD10G1000500.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0016165
Physical Location & Seq
Reverse (-)
54074 .. 55818
1745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1000500.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGAGAAATATTATTATGATTTTGCTGCTGGTGCTGCTTGTGCAGTGCTGCTGCTCAAATACTGGGGCAATCTCGACGATGACTACTAGTAGTAGTGTGGGGTCAGCGCAGGTTCCAGCAATGTTTGTGTTTGGGGACTCATTGATTGATGTTGGCAACAACAACTTCCTCAACTCCCTTGCCAAATCCAACTATTATCCTTATGGCTGCGATTTCAGGGCAGGCCCTACTGGCAGATTCACTAATGGAAGAACTTTTGTTGATCTCCTTGGGAATTTGCTGGGTCTCCCATATGTTCCAGCCTTCGCTGATCCTAATACAAAAGGTACCAGAATGCTTGGGGGAGTAAATTATGCTTCAGCAGCTGCTGGCATACTTGATGAGACAGGCCAACACTATGGAGAACGGTATAGACTGAGCCAGCAAGTATTGAACTTTGAGAGCACCTTGAATCAGTTGAGATCGATGATGAGTGGAACCAACCTGACCCAAATGCTGGCCAAGTCCATAGCAGTTTTGGCGTTTGGGAGCAATGACTACATCAACAACTACCTCTTGCCTTCCCTCTATACTTCCAGCTACTACTACACTCCTCCTGCCTTCGCCAACCTTCTCCTTAATCGCTATACTCGACAAATTCTGGCTTTGCACAGTGTAGGATTAAGAAAGTTTGTGCTAGCAGGAATTGGGCCATTGGGCTGCATACCAAACCAAAGAGCCTCAGCGGCCCAGCCCGAAAGGTGCTTGGACTATGTGAATCAAATCCTTGGTACCTACAATGAAGGCCTTAGATCACTCGTCAACCAATTCAACACCAACCATCCTGATGCCATCTTTGTCTACGCCAACACTTATGGTGCTTTTGGTGACATGCTCAATAATCCTGCCCCCTACGGGTTCAGTGTGATTGATAGGGGATGCTGTGGAATTGGGAGGAACAGAGGGCAGATAACATGCCTACCTTATGCAGTTCCATGCTCTAATAGAAATCAATACATGTTTTGGGATGCATTCCACCCAACAGAAGCTGCAAATGCCATCCTTGCTTGGCGGGCTTATAACGGCCCACCCTCTGATTCTTATCCAATCAACGTCCAGCAAATGGCTCTGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

372

Amino Acids

40.62

Weight (kDa)

8.47

Isoelectric Point (pI)

27.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 349 6.5e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71250
fragaria_vesca FvH4_2g00180
malus_domestica MD10G1000500.v1.1
prunus_persica Prupe.8G001600_v2.0.a1 Prupe.8G001600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249471
rosa_laevigata RLG00000015578
rosa_multiflora Rmu_ssc0000459.1_g000021
rosa_roxburghii Rroxscaffold_7G00217670
rosa_rugosa Rorug05G0492600
rosa_samantha Rh6AG002300 Rh6BG007800 Rh6CG001500 Rh6DG001500
rosa_wichuraiana Rw6G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
Acc36I ACCTGC 1 cut(s) 100
Acc65I GGTACC 2 cut(s) 326, 770
AccB1I GGYRCC 2 cut(s) 326, 770
AccB7I CCANNNNNTGG 2 cut(s) 496, 1102
AccI GTMKAC 1 cut(s) 840
AciI CCGC 2 cut(s) 725, 1051
AclWI GGATC 1 cut(s) 305
AcoI YGGCCR 1 cut(s) 498
AcsI RAATTY 2 cut(s) 274, 636
AcuI CTGAAG 1 cut(s) 342
AfaI GTAC 2 cut(s) 328, 772
AfiI CCNNNNNNNGG 3 cut(s) 496, 894, 1102
AflIII ACRYGT 1 cut(s) 996
AgsI TTSAA 3 cut(s) 433, 451, 811
AhlI ACTAGT 1 cut(s) 86
AluBI AGCT 3 cut(s) 365, 579, 1028
AluI AGCT 3 cut(s) 365, 579, 1028
Alw21I GWGCWC 1 cut(s) 446
Alw26I GTCTC 2 cut(s) 290, 377
AlwI GGATC 1 cut(s) 305
AlwNI CAGNNNCTG 3 cut(s) 365, 368, 1028
AoxI GGCC 7 cut(s) 223, 388, 498, 689, 726, 784, 1063
ApeKI GCWGC 9 cut(s) 25, 34, 48, 51, 207, 362, 365, 699, 1028
ApoI RAATTY 2 cut(s) 274, 636
Asp718I GGTACC 2 cut(s) 326, 770
AspLEI GCGC 1 cut(s) 109
AspS9I GGNCC 4 cut(s) 224, 689, 727, 1064
AsuHPI GGTGA 1 cut(s) 878
AsuNHI GCTAGC 1 cut(s) 676
BalI TGGCCA 1 cut(s) 500
BanI GGYRCC 2 cut(s) 326, 770
Bbv12I GWGCWC 1 cut(s) 446
BbvCI CCTCAGC 1 cut(s) 721
BbvI GCAGC 9 cut(s) 12, 21, 35, 38, 194, 352, 374, 686, 1015
BccI CCATC 3 cut(s) 828, 839, 1046
BceAI ACGGC 1 cut(s) 1078
BcoDI GTCTC 2 cut(s) 290, 377
BcuI ACTAGT 1 cut(s) 86
BfaI CTAG 2 cut(s) 87, 677
BfuAI ACCTGC 1 cut(s) 100
BglI GCCNNNNNGGC 2 cut(s) 231, 725
BmgT120I GGNCC 4 cut(s) 224, 689, 727, 1064
BmiI GGNNCC 4 cut(s) 114, 328, 478, 772
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 3 cut(s) 817, 908, 997
BmtI GCTAGC 1 cut(s) 680
BmuI ACTGGG 1 cut(s) 72
Bpu10I CCTNAGC 1 cut(s) 721
Bsa29I ATCGAT 1 cut(s) 464
BsaBI GATNNNNATC 1 cut(s) 1080
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 2 cut(s) 268, 766
Bsc4I CCNNNNNNNGG 3 cut(s) 496, 894, 1102
Bse1I ACTGG 2 cut(s) 67, 235
Bse3DI GCAATG 2 cut(s) 126, 538
Bse8I GATNNNNATC 1 cut(s) 1080
BseCI ATCGAT 1 cut(s) 464
BseDI CCNNGG 2 cut(s) 268, 766
BseGI GGATG 4 cut(s) 820, 923, 1012, 1038
BseJI GATNNNNATC 1 cut(s) 1080
BseLI CCNNNNNNNGG 3 cut(s) 496, 894, 1102
BseMI GCAATG 2 cut(s) 126, 538
BseMII CTCAG 2 cut(s) 407, 735
BseNI ACTGG 2 cut(s) 67, 235
BseRI GAGGAG 1 cut(s) 582
BseXI GCAGC 9 cut(s) 12, 21, 35, 38, 194, 352, 374, 686, 1015
BseYI CCCAGC 2 cut(s) 280, 729
BsgI GTGCAG 1 cut(s) 62
BshFI GGCC 7 cut(s) 225, 390, 500, 691, 728, 786, 1065
BshNI GGYRCC 2 cut(s) 326, 770
BshVI ATCGAT 1 cut(s) 464
BsiHKAI GWGCWC 1 cut(s) 446
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 3 cut(s) 496, 894, 1102
BsmAI GTCTC 2 cut(s) 290, 377
BsmFI GGGAC 1 cut(s) 149
BsmI GAATGC 2 cut(s) 339, 1010
BsnI GGCC 7 cut(s) 225, 390, 500, 691, 728, 786, 1065
Bso31I GGTCTC 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 446
Bsp143I GATC 5 cut(s) 262, 310, 461, 791, 1110
BspACI CCGC 2 cut(s) 725, 1051
BspANI GGCC 7 cut(s) 225, 390, 500, 691, 728, 786, 1065
BspCNI CTCAG 2 cut(s) 408, 734
BspDI ATCGAT 1 cut(s) 464
BspLI GGNNCC 4 cut(s) 114, 328, 478, 772
BspMI ACCTGC 1 cut(s) 100
BspOI GCTAGC 1 cut(s) 680
BspPI GGATC 1 cut(s) 305
BspT107I GGYRCC 2 cut(s) 326, 770
BspTNI GGTCTC 1 cut(s) 290
BsrDI GCAATG 2 cut(s) 126, 538
BsrI ACTGG 2 cut(s) 67, 235
BssECI CCNNGG 2 cut(s) 268, 766
BssMI GATC 5 cut(s) 262, 310, 461, 791, 1110
BssT1I CCWWGG 2 cut(s) 268, 766
Bst4CI ACNGT 2 cut(s) 408, 653
BstC8I GCNNGC 6 cut(s) 223, 370, 422, 498, 678, 1053
BstDEI CTNAG 3 cut(s) 416, 721, 788
BstF5I GGATG 4 cut(s) 820, 923, 1012, 1038
BstHHI GCGC 1 cut(s) 109
BstKTI GATC 5 cut(s) 265, 313, 464, 794, 1113
BstMAI GTCTC 2 cut(s) 290, 377
BstMBI GATC 5 cut(s) 262, 310, 461, 791, 1110
BstNSI RCATGY 3 cut(s) 874, 957, 1000
BstV1I GCAGC 9 cut(s) 12, 21, 35, 38, 194, 352, 374, 686, 1015
BstXI CCANNNNNNTGG 1 cut(s) 398
Bsu15I ATCGAT 1 cut(s) 464
BsuRI GGCC 7 cut(s) 225, 390, 500, 691, 728, 786, 1065
BsuTUI ATCGAT 1 cut(s) 464
BtsCI GGATG 4 cut(s) 820, 923, 1012, 1038
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 3 cut(s) 50, 658, 907
BveI ACCTGC 1 cut(s) 100
Cac8I GCNNGC 6 cut(s) 223, 370, 422, 498, 678, 1053
CaiI CAGNNNCTG 3 cut(s) 365, 368, 1028
CfoI GCGC 1 cut(s) 109
Cfr13I GGNCC 4 cut(s) 224, 689, 727, 1064
ClaI ATCGAT 1 cut(s) 464
Csp6I GTAC 2 cut(s) 327, 771
CviAII CATG 4 cut(s) 871, 954, 975, 997
CviQI GTAC 2 cut(s) 327, 771
DdeI CTNAG 3 cut(s) 416, 721, 788
DpnI GATC 5 cut(s) 264, 312, 463, 793, 1112
DpnII GATC 5 cut(s) 262, 310, 461, 791, 1110
EaeI YGGCCR 1 cut(s) 498
Eco130I CCWWGG 2 cut(s) 268, 766
Eco147I AGGCCT 1 cut(s) 786
Eco31I GGTCTC 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 342
EcoO109I RGGNCCY 1 cut(s) 224
EcoT14I CCWWGG 2 cut(s) 268, 766
EcoT22I ATGCAT 1 cut(s) 1012
ErhI CCWWGG 2 cut(s) 268, 766
FaeI CATG 4 cut(s) 874, 957, 978, 1000
FaqI GGGAC 1 cut(s) 149
FatI CATG 4 cut(s) 870, 953, 974, 996
FauI CCCGC 1 cut(s) 1044
FauNDI CATATG 1 cut(s) 292
FblI GTMKAC 1 cut(s) 840
FokI GGATG 4 cut(s) 807, 930, 1019, 1025
FspBI CTAG 2 cut(s) 87, 677
GlaI GCGC 1 cut(s) 108
GsaI CCCAGC 2 cut(s) 284, 733
HaeIII GGCC 7 cut(s) 225, 390, 500, 691, 728, 786, 1065
HhaI GCGC 1 cut(s) 109
Hin1II CATG 4 cut(s) 874, 957, 978, 1000
Hin6I GCGC 1 cut(s) 107
HinP1I GCGC 1 cut(s) 107
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HinfI GANTC 5 cut(s) 137, 237, 451, 757, 1076
HphI GGTGA 1 cut(s) 878
Hpy166II GTNNAC 2 cut(s) 802, 841
Hpy188I TCNGA 3 cut(s) 1075, 1110, 1115
Hpy188III TCNNGA 2 cut(s) 73, 824
Hpy8I GTNNAC 2 cut(s) 802, 841
Hpy99I CGWCG 1 cut(s) 79
HpyAV CCTTC 5 cut(s) 313, 570, 610, 620, 776
HpyCH4III ACNGT 2 cut(s) 408, 653
HpyCH4IV ACGT 1 cut(s) 1092
HpyCH4V TGCA 6 cut(s) 43, 649, 702, 968, 1010, 1031
HpyF3I CTNAG 3 cut(s) 416, 721, 788
HpySE526I ACGT 1 cut(s) 1092
Hsp92II CATG 4 cut(s) 874, 957, 978, 1000
HspAI GCGC 1 cut(s) 107
KpnI GGTACC 2 cut(s) 330, 774
Kzo9I GATC 5 cut(s) 262, 310, 461, 791, 1110
LmnI GCTCC 1 cut(s) 528
Lsp1109I GCAGC 9 cut(s) 12, 21, 35, 38, 194, 352, 374, 686, 1015
LweI GCATC 3 cut(s) 817, 908, 997
MaeI CTAG 2 cut(s) 87, 677
MaeII ACGT 1 cut(s) 1092
MaeIII GTNAC 1 cut(s) 866
MalI GATC 5 cut(s) 264, 312, 463, 793, 1112
MboI GATC 5 cut(s) 262, 310, 461, 791, 1110
MboII GAAGA 1 cut(s) 261
MhlI GDGCHC 1 cut(s) 446
MlsI TGGCCA 1 cut(s) 500
MluCI AATT 6 cut(s) 274, 349, 636, 684, 806, 927
MluNI TGGCCA 1 cut(s) 500
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 8 cut(s) 179, 563, 575, 603, 730, 927, 935, 1081
Mox20I TGGCCA 1 cut(s) 500
Mph1103I ATGCAT 1 cut(s) 1012
MscI TGGCCA 1 cut(s) 500
MseI TTAA 2 cut(s) 618, 662
MslI CAYNNNNRTG 1 cut(s) 825
Msp20I TGGCCA 1 cut(s) 500
MspA1I CMGCKG 2 cut(s) 365, 725
Mva1269I GAATGC 2 cut(s) 339, 1010
NdeI CATATG 1 cut(s) 292
NdeII GATC 5 cut(s) 262, 310, 461, 791, 1110
NheI GCTAGC 1 cut(s) 676
NlaIII CATG 4 cut(s) 874, 957, 978, 1000
NlaIV GGNNCC 4 cut(s) 114, 328, 478, 772
NmuCI GTSAC 1 cut(s) 866
NsiI ATGCAT 1 cut(s) 1012
NspI RCATGY 3 cut(s) 874, 957, 1000
PceI AGGCCT 1 cut(s) 786
PciI ACATGT 1 cut(s) 996
PcsI WCGNNNNNNNCGW 1 cut(s) 628
PctI GAATGC 2 cut(s) 339, 1010
PfeI GAWTC 4 cut(s) 237, 451, 757, 1076
PflMI CCANNNNNTGG 2 cut(s) 496, 1102
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
PscI ACATGT 1 cut(s) 996
PsiI TTATAA 1 cut(s) 1059
PspFI CCCAGC 2 cut(s) 280, 729
PspN4I GGNNCC 4 cut(s) 114, 328, 478, 772
PspPI GGNCC 4 cut(s) 224, 689, 727, 1064
PstNI CAGNNNCTG 3 cut(s) 365, 368, 1028
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 2 cut(s) 328, 772
RsaNI GTAC 2 cut(s) 327, 771
RseI CAYNNNNRTG 1 cut(s) 825
SaqAI TTAA 2 cut(s) 618, 662
Sau3AI GATC 5 cut(s) 262, 310, 461, 791, 1110
Sau96I GGNCC 4 cut(s) 224, 689, 727, 1064
SchI GAGTC 1 cut(s) 131
SduI GDGCHC 1 cut(s) 446
SfaNI GCATC 3 cut(s) 817, 908, 997
SmiMI CAYNNNNRTG 1 cut(s) 825
SpeI ACTAGT 1 cut(s) 86
Sse9I AATT 6 cut(s) 274, 349, 636, 684, 806, 927
SseBI AGGCCT 1 cut(s) 786
SsiI CCGC 2 cut(s) 725, 1051
SspI AATATT 1 cut(s) 10
SspMI CTAG 2 cut(s) 87, 677
StuI AGGCCT 1 cut(s) 786
StyI CCWWGG 2 cut(s) 268, 766
TaaI ACNGT 2 cut(s) 408, 653
TaiI ACGT 1 cut(s) 1095
TaqI TCGA 3 cut(s) 74, 464, 631
TasI AATT 6 cut(s) 274, 349, 636, 684, 806, 927
TauI GCSGC 1 cut(s) 728
TfiI GAWTC 4 cut(s) 237, 451, 757, 1076
Tru1I TTAA 2 cut(s) 618, 662
Tru9I TTAA 2 cut(s) 618, 662
TscAI CASTG 3 cut(s) 50, 658, 907
TseFI GTSAC 1 cut(s) 866
TseI GCWGC 9 cut(s) 25, 34, 48, 51, 207, 362, 365, 699, 1028
Tsp45I GTSAC 1 cut(s) 866
TspDTI ATGAA 1 cut(s) 795
TspRI CASTG 3 cut(s) 50, 658, 907
Van91I CCANNNNNTGG 2 cut(s) 496, 1102
XapI RAATTY 2 cut(s) 274, 636
XceI RCATGY 3 cut(s) 874, 957, 1000
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 840
XspI CTAG 2 cut(s) 87, 677
Zsp2I ATGCAT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.