FvH4_2g00180

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016165
Physical Location & Seq
Reverse (-)
285002 .. 287763
2762 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00180.t1

Sequence Viewer

Length: 1158 bp
ATGCATGCCTGCATTGGGGGTGAGAAGATGGTAGCGATTTGTAATGAACGGCTGCGCTTAGCCAACTATACTATCAGCGTGGTGATTATGAAGATCTTAATTAGTTGTAGTATGGTAGCAGGCGTGGGATTTGGGGAGTCAGCAGCAGCGCAGGTTCCGGCAATGTTTGCGTTTGGGGACTCACTCATTGATGTTGGCAACAACAACTTCATAAGCTCCTTCGCAAGATCCAATTACTATCCTTATGGCATCGATTTCAGGGGAGGCCCCTCCGGCAGATTCACCAATGGAAGAACTTCCATTGATATGCTTGGAAATTTGCTGGGTCTTCCTTATGCTCCGGCCTTTGCAGATCCTAATACAAAAGGAAACAGAATACTCAGGGGAGTCAATTATGCCTCAGCAGCTGCTGGCATACTCGATGAGACAGGGCGACACTACGGAGATCGGTACTCCCTAAGCCGACAAGTAATGAATTTCGAAAGCACCTTGAATCAGTTGAGAACGATGATGGGGGGAACTAACCTGACCCAGTACCTGGCTAAGTCGATTGCTGTTTTGGTGTTTGGGAGCAATGACTACATCAACAACTACCTCATGCCCTTCTATAATTCCAGTTACACTTATAGTCCTCCTGCCTTTGCCAACCTTCTCCTGAGTCGCTATGCCACACAGATTCTGGCTTTGCACAGTCTTGGATTGAGGAAGTTTATGCTGGTAGGAATCGGGCCTTTGGGTTGCATTCCTAACCAAAGAGCATCAGGTAGAGCCCAGCCTGGAAGATGTGTTGACAATGTCAATCAGATCCTTGGCAGCTTCAATCAAGGGCTCAGATCACTCGCCACCCAGCTTAACAGCAACCATCCTGGTGCCATCTTTGTGTACGGCAATGCTTATGGCGCTTTTGGCGACATTCTGAACAATCCTTTATCTTATGGTTTCACTGTGATTGATAGGGCTTGCTGTGGAATTGGGAGGAACCAAGGGCAGATAACATGTCTTCCATTTGCAGTTCCATGCTCAAATAGAAATCAATATATGTTTTGGGATGCTTTCCACCCAACTGAAGCTGCAAACGCAGTCCTTGCTTGGCGGGCCTTTAACGGGCCACCCTCTGATAGTTACCCCATCAATGTCCAACAAATGTCTCTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

386

Amino Acids

41.81

Weight (kDa)

9.02

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 56 - 363 2.4e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016165)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71250
fragaria_vesca FvH4_2g00180
malus_domestica MD10G1000500.v1.1
prunus_persica Prupe.8G001600_v2.0.a1 Prupe.8G001600_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249471
rosa_laevigata RLG00000015578
rosa_multiflora Rmu_ssc0000459.1_g000021
rosa_roxburghii Rroxscaffold_7G00217670
rosa_rugosa Rorug05G0492600
rosa_samantha Rh6AG002300 Rh6BG007800 Rh6CG001500 Rh6DG001500
rosa_wichuraiana Rw6G000290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1156
Acc36I ACCTGC 1 cut(s) 142
AccB1I GGYRCC 1 cut(s) 869
AccB7I CCANNNNNTGG 1 cut(s) 538
AciI CCGC 1 cut(s) 1093
AclWI GGATC 3 cut(s) 222, 347, 799
AcsI RAATTY 2 cut(s) 316, 475
AcuI CTGAAG 1 cut(s) 1086
AfaI GTAC 3 cut(s) 452, 536, 884
AfiI CCNNNNNNNGG 3 cut(s) 15, 538, 1104
AflIII ACRYGT 1 cut(s) 995
AgsI TTSAA 2 cut(s) 493, 820
AjnI CCWGG 3 cut(s) 537, 775, 865
AluBI AGCT 5 cut(s) 216, 407, 816, 850, 1070
AluI AGCT 5 cut(s) 216, 407, 816, 850, 1070
Alw26I GTCTC 2 cut(s) 419, 1152
AlwI GGATC 3 cut(s) 222, 347, 799
AlwNI CAGNNNCTG 4 cut(s) 407, 410, 538, 679
AoxI GGCC 5 cut(s) 265, 342, 728, 1095, 1106
ApeKI GCWGC 7 cut(s) 52, 143, 146, 404, 407, 813, 1070
ApoI RAATTY 2 cut(s) 316, 475
Asp700I GAANNNNTTC 1 cut(s) 295
AspLEI GCGC 3 cut(s) 57, 151, 902
AspS9I GGNCC 4 cut(s) 266, 728, 1095, 1106
AsuHPI GGTGA 3 cut(s) 32, 94, 274
AsuII TTCGAA 1 cut(s) 480
BanI GGYRCC 1 cut(s) 869
BanII GRGCYC 2 cut(s) 772, 831
BbsI GAAGAC 2 cut(s) 320, 992
BbvCI CCTCAGC 1 cut(s) 400
BbvI GCAGC 7 cut(s) 39, 155, 158, 394, 416, 825, 1057
BccI CCATC 5 cut(s) 22, 505, 870, 881, 1136
BceAI ACGGC 2 cut(s) 65, 901
BciT130I CCWGG 3 cut(s) 539, 777, 867
BcoDI GTCTC 2 cut(s) 419, 1152
BfoI RGCGCY 1 cut(s) 903
BfuAI ACCTGC 1 cut(s) 142
BglI GCCNNNNNGGC 1 cut(s) 273
BglII AGATCT 1 cut(s) 93
BisI GCNGC 7 cut(s) 53, 144, 147, 405, 408, 814, 1071
BlpI GCTNAGC 1 cut(s) 58
BlsI GCNGC 7 cut(s) 54, 145, 148, 406, 409, 815, 1072
Bme1390I CCNGG 3 cut(s) 539, 777, 867
BmgT120I GGNCC 4 cut(s) 266, 728, 1095, 1106
BmiI GGNNCC 4 cut(s) 156, 268, 871, 980
BmrFI CCNGG 3 cut(s) 539, 777, 867
BmrI ACTGGG 1 cut(s) 526
BmsI GCATC 3 cut(s) 258, 767, 1039
BmuI ACTGGG 1 cut(s) 526
BpiI GAAGAC 2 cut(s) 320, 992
Bpu10I CCTNAGC 2 cut(s) 400, 458
Bpu1102I GCTNAGC 1 cut(s) 58
Bpu14I TTCGAA 1 cut(s) 480
Bsa29I ATCGAT 1 cut(s) 252
BsaJI CCNNGG 2 cut(s) 808, 982
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 538, 1104
Bse1I ACTGG 2 cut(s) 532, 615
Bse3DI GCAATG 3 cut(s) 168, 580, 895
BseBI CCWGG 3 cut(s) 539, 777, 867
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 808, 982
BseGI GGATG 2 cut(s) 862, 1054
BseLI CCNNNNNNNGG 3 cut(s) 15, 538, 1104
BseMI GCAATG 3 cut(s) 168, 580, 895
BseMII CTCAG 4 cut(s) 394, 414, 647, 844
BseNI ACTGG 2 cut(s) 532, 615
BseXI GCAGC 7 cut(s) 39, 155, 158, 394, 416, 825, 1057
BseYI CCCAGC 3 cut(s) 322, 771, 846
BshFI GGCC 5 cut(s) 267, 344, 730, 1097, 1108
BshNI GGYRCC 1 cut(s) 869
BshVI ATCGAT 1 cut(s) 252
BsiSI CCGG 3 cut(s) 158, 273, 341
BslFI GGGAC 1 cut(s) 191
BslI CCNNNNNNNGG 3 cut(s) 15, 538, 1104
BsmAI GTCTC 2 cut(s) 419, 1152
BsmFI GGGAC 1 cut(s) 191
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 5 cut(s) 267, 344, 730, 1097, 1108
Bsp119I TTCGAA 1 cut(s) 480
Bsp1286I GDGCHC 2 cut(s) 772, 831
Bsp143I GATC 6 cut(s) 93, 227, 352, 445, 804, 833
Bsp1720I GCTNAGC 1 cut(s) 58
BspACI CCGC 1 cut(s) 1093
BspANI GGCC 5 cut(s) 267, 344, 730, 1097, 1108
BspCNI CTCAG 4 cut(s) 393, 413, 648, 843
BspDI ATCGAT 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 156, 268, 871, 980
BspMI ACCTGC 1 cut(s) 142
BspPI GGATC 3 cut(s) 222, 347, 799
BspT104I TTCGAA 1 cut(s) 480
BspT107I GGYRCC 1 cut(s) 869
BsrDI GCAATG 3 cut(s) 168, 580, 895
BsrI ACTGG 2 cut(s) 532, 615
BssECI CCNNGG 2 cut(s) 808, 982
BssMI GATC 6 cut(s) 93, 227, 352, 445, 804, 833
BssT1I CCWWGG 2 cut(s) 808, 982
Bst2UI CCWGG 3 cut(s) 539, 777, 867
Bst4CI ACNGT 2 cut(s) 692, 946
BstAPI GCANNNNNTGC 2 cut(s) 167, 1085
BstBI TTCGAA 1 cut(s) 480
BstC8I GCNNGC 6 cut(s) 6, 10, 121, 412, 961, 1095
BstDEI CTNAG 7 cut(s) 58, 380, 400, 458, 543, 656, 830
BstF5I GGATG 2 cut(s) 862, 1054
BstH2I RGCGCY 1 cut(s) 903
BstHHI GCGC 3 cut(s) 57, 151, 902
BstKTI GATC 6 cut(s) 96, 230, 355, 448, 807, 836
BstMAI GTCTC 2 cut(s) 419, 1152
BstMBI GATC 6 cut(s) 93, 227, 352, 445, 804, 833
BstMWI GCNNNNNNNGC 8 cut(s) 167, 273, 404, 899, 906, 1076, 1085, 1094
BstNI CCWGG 3 cut(s) 539, 777, 867
BstNSI RCATGY 2 cut(s) 8, 999
BstSCI CCNGG 3 cut(s) 537, 775, 865
BstV1I GCAGC 7 cut(s) 39, 155, 158, 394, 416, 825, 1057
BstV2I GAAGAC 2 cut(s) 320, 992
BstX2I RGATCY 4 cut(s) 93, 227, 352, 804
BstYI RGATCY 4 cut(s) 93, 227, 352, 804
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 5 cut(s) 267, 344, 730, 1097, 1108
BsuTUI ATCGAT 1 cut(s) 252
BtsCI GGATG 2 cut(s) 862, 1054
BtsIMutI CAGTG 1 cut(s) 942
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 6 cut(s) 6, 10, 121, 412, 961, 1095
CaiI CAGNNNCTG 4 cut(s) 407, 410, 538, 679
CfoI GCGC 3 cut(s) 57, 151, 902
Cfr13I GGNCC 4 cut(s) 266, 728, 1095, 1106
ClaI ATCGAT 1 cut(s) 252
Csp6I GTAC 3 cut(s) 451, 535, 883
CviAII CATG 4 cut(s) 5, 598, 996, 1017
CviQI GTAC 3 cut(s) 451, 535, 883
DdeI CTNAG 7 cut(s) 58, 380, 400, 458, 543, 656, 830
DpnI GATC 6 cut(s) 95, 229, 354, 447, 806, 835
DpnII GATC 6 cut(s) 93, 227, 352, 445, 804, 833
Eco130I CCWWGG 2 cut(s) 808, 982
Eco24I GRGCYC 2 cut(s) 772, 831
Eco57I CTGAAG 1 cut(s) 1086
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 3 cut(s) 537, 775, 865
EcoT14I CCWWGG 2 cut(s) 808, 982
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 772, 831
ErhI CCWWGG 2 cut(s) 808, 982
FaeI CATG 4 cut(s) 8, 601, 999, 1020
FaqI GGGAC 1 cut(s) 191
FatI CATG 4 cut(s) 4, 597, 995, 1016
FauI CCCGC 1 cut(s) 1086
Fnu4HI GCNGC 7 cut(s) 53, 144, 147, 405, 408, 814, 1071
FokI GGATG 2 cut(s) 849, 1061
FriOI GRGCYC 2 cut(s) 772, 831
Fsp4HI GCNGC 7 cut(s) 53, 144, 147, 405, 408, 814, 1071
GlaI GCGC 3 cut(s) 56, 150, 901
GluI GCNGC 7 cut(s) 53, 144, 147, 405, 408, 814, 1071
GsaI CCCAGC 3 cut(s) 326, 775, 850
HaeII RGCGCY 1 cut(s) 903
HaeIII GGCC 5 cut(s) 267, 344, 730, 1097, 1108
HapII CCGG 3 cut(s) 158, 273, 341
HhaI GCGC 3 cut(s) 57, 151, 902
Hin1II CATG 4 cut(s) 8, 601, 999, 1020
Hin6I GCGC 3 cut(s) 55, 149, 900
HinP1I GCGC 3 cut(s) 55, 149, 900
HincII GTYRAC 1 cut(s) 790
HindII GTYRAC 1 cut(s) 790
HinfI GANTC 8 cut(s) 137, 179, 279, 387, 493, 658, 676, 723
HpaII CCGG 3 cut(s) 158, 273, 341
HphI GGTGA 3 cut(s) 32, 94, 274
Hpy166II GTNNAC 2 cut(s) 790, 883
Hpy188I TCNGA 4 cut(s) 804, 833, 918, 1117
Hpy188III TCNNGA 1 cut(s) 655
Hpy8I GTNNAC 2 cut(s) 790, 883
HpyAV CCTTC 3 cut(s) 229, 613, 659
HpyCH4III ACNGT 2 cut(s) 692, 946
HpyCH4V TGCA 7 cut(s) 4, 12, 350, 688, 741, 1010, 1073
HpyF10VI GCNNNNNNNGC 8 cut(s) 167, 273, 404, 899, 906, 1076, 1085, 1094
HpyF3I CTNAG 7 cut(s) 58, 380, 400, 458, 543, 656, 830
Hsp92II CATG 4 cut(s) 8, 601, 999, 1020
HspAI GCGC 3 cut(s) 55, 149, 900
Kzo9I GATC 6 cut(s) 93, 227, 352, 445, 804, 833
LmnI GCTCC 3 cut(s) 221, 343, 570
Lsp1109I GCAGC 7 cut(s) 39, 155, 158, 394, 416, 825, 1057
LweI GCATC 3 cut(s) 258, 767, 1039
MaeIII GTNAC 2 cut(s) 617, 1121
MalI GATC 6 cut(s) 95, 229, 354, 447, 806, 835
MboI GATC 6 cut(s) 93, 227, 352, 445, 804, 833
MboII GAAGA 6 cut(s) 37, 103, 303, 320, 792, 992
MflI RGATCY 4 cut(s) 93, 227, 352, 804
MhlI GDGCHC 2 cut(s) 772, 831
MluCI AATT 7 cut(s) 99, 232, 316, 391, 475, 610, 969
MlyI GAGTC 4 cut(s) 146, 173, 396, 667
MnlI CCTC 8 cut(s) 257, 280, 409, 605, 642, 696, 969, 1123
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 295
MseI TTAA 3 cut(s) 98, 852, 1101
MslI CAYNNNNRTG 3 cut(s) 305, 867, 878
MspA1I CMGCKG 1 cut(s) 407
MspI CCGG 3 cut(s) 158, 273, 341
MspR9I CCNGG 3 cut(s) 539, 777, 867
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 3 cut(s) 539, 777, 867
MwoI GCNNNNNNNGC 8 cut(s) 167, 273, 404, 899, 906, 1076, 1085, 1094
NdeII GATC 6 cut(s) 93, 227, 352, 445, 804, 833
NlaIII CATG 4 cut(s) 8, 601, 999, 1020
NlaIV GGNNCC 4 cut(s) 156, 268, 871, 980
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 999
NspV TTCGAA 1 cut(s) 480
PaeI GCATGC 1 cut(s) 8
PciI ACATGT 1 cut(s) 995
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 295
PfeI GAWTC 4 cut(s) 279, 493, 676, 723
PflFI GACNNNGTC 1 cut(s) 794
PflMI CCANNNNNTGG 1 cut(s) 538
PkrI GCNGC 7 cut(s) 54, 145, 148, 406, 409, 815, 1072
PleI GAGTC 4 cut(s) 145, 173, 395, 666
PpsI GAGTC 4 cut(s) 145, 173, 395, 666
PscI ACATGT 1 cut(s) 995
PsiI TTATAA 1 cut(s) 1156
Psp6I CCWGG 3 cut(s) 537, 775, 865
PspFI CCCAGC 3 cut(s) 322, 771, 846
PspGI CCWGG 3 cut(s) 537, 775, 865
PspN4I GGNNCC 4 cut(s) 156, 268, 871, 980
PspPI GGNCC 4 cut(s) 266, 728, 1095, 1106
PstNI CAGNNNCTG 4 cut(s) 407, 410, 538, 679
PsuI RGATCY 4 cut(s) 93, 227, 352, 804
PsyI GACNNNGTC 1 cut(s) 794
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 3 cut(s) 452, 536, 884
RsaNI GTAC 3 cut(s) 451, 535, 883
RseI CAYNNNNRTG 3 cut(s) 305, 867, 878
SaqAI TTAA 3 cut(s) 98, 852, 1101
SatI GCNGC 7 cut(s) 53, 144, 147, 405, 408, 814, 1071
Sau3AI GATC 6 cut(s) 93, 227, 352, 445, 804, 833
Sau96I GGNCC 4 cut(s) 266, 728, 1095, 1106
SchI GAGTC 4 cut(s) 146, 173, 396, 667
ScrFI CCNGG 3 cut(s) 539, 777, 867
SduI GDGCHC 2 cut(s) 772, 831
SfaNI GCATC 3 cut(s) 258, 767, 1039
SfuI TTCGAA 1 cut(s) 480
SmiMI CAYNNNNRTG 3 cut(s) 305, 867, 878
SphI GCATGC 1 cut(s) 8
Sse9I AATT 7 cut(s) 99, 232, 316, 391, 475, 610, 969
SsiI CCGC 1 cut(s) 1093
StyD4I CCNGG 3 cut(s) 537, 775, 865
StyI CCWWGG 2 cut(s) 808, 982
TaaI ACNGT 2 cut(s) 692, 946
TaqI TCGA 4 cut(s) 252, 420, 480, 548
TasI AATT 7 cut(s) 99, 232, 316, 391, 475, 610, 969
TfiI GAWTC 4 cut(s) 279, 493, 676, 723
Tru1I TTAA 3 cut(s) 98, 852, 1101
Tru9I TTAA 3 cut(s) 98, 852, 1101
TscAI CASTG 1 cut(s) 949
TseI GCWGC 7 cut(s) 52, 143, 146, 404, 407, 813, 1070
TspDTI ATGAA 4 cut(s) 60, 104, 199, 488
TspGWI ACGGA 1 cut(s) 456
TspRI CASTG 1 cut(s) 949
Tth111I GACNNNGTC 1 cut(s) 794
Van91I CCANNNNNTGG 1 cut(s) 538
XapI RAATTY 2 cut(s) 316, 475
XceI RCATGY 2 cut(s) 8, 999
XcmI CCANNNNNNNNNTGG 1 cut(s) 676
XmnI GAANNNNTTC 1 cut(s) 295
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.