AT1G74220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010088
Physical Location & Seq
Reverse (-)
27912931 .. 27914161
1231 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74220.1

Sequence Viewer

Length: 951 bp
ATGGAATCATCACAAGAACAAAGTTTCTTTAGCCCCACGAACCAAAGCTCGAGAAACTTAGCATCTAAACCTGAAACAGAAGATCCAATCATAAGACTTTCTTCTTCGTCTTCCTCTTCTTGTTCTTCCATAGAAGCCGAACCAAGACCTGATCAGAATCACAACCACAACAATGTAGAGCAATCTCCTCCGACACAAGTCATGGAAAGATCAACAAACAACACAACAACAACGACGACCTCTACCCCTAATACACCTCCTTACAGAATCCCTTCACATGTTTTCGCGAGAACAACCTCTACAGCTCCAGAATGGAGTACTATCTCCAATGAATCTCTTTTCAGCATCCATATGGGTAACAATAGCTTCACCGGTGGAGATTATTTCAAATCCGGCGAACTCACGTTTCCTCAGCCTCCTTCACCAATAACTTCTCCTTTGCCTTCTCCTTTACCATCTACGCCCCACAACGTGAACCAAGGAGGAGCCGTAGGAGCTGCTGCGGAGGTAAAAACTCCGGTGGATATAGGCAAGAAAGCAGCTGAAACAGATAAAGCTTACCGCGCAAGTAAAGAAGACGAACAAAAAGCTGCAGCAAGTATAAGAGAGGTTATCATGGCTAATGAAGCTGCTAACAACAACAACAAAACCAATAAACTAGATCGTTCTGTTTCTCGTCGCTCAGAAAATTTAAGTGTCAAGTCTTTCGCTTTTCCAGTAATGGGGAATGCAGACAAAGGCGGGTTACAAGGTTCCACGCCTGAAAAGAAGCAGAGGAATCCTTCGCAGCCAGAGACACCGAAGATTTCCACTGAAGCTGAAGGAGATGTAGGATTGAAGAAAGAGGAAGCTCCTAAACCAGTAGCTCCTAAAACAGAAGCGGACCGTGCCTCTGATCGCAACCCCAAATGGCTCTCTTGCTTCCCTTGCTGTACAACCTCTTGTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

316

Amino Acids

34.05

Weight (kDa)

6.26

Isoelectric Point (pI)

62.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 287, 564
AciI CCGC 4 cut(s) 503, 562, 741, 881
AclWI GGATC 1 cut(s) 77
AcsI RAATTY 1 cut(s) 688
AcuI CTGAAG 2 cut(s) 834, 840
AdeI CACNNNGTG 1 cut(s) 472
AfaI GTAC 2 cut(s) 319, 934
AfiI CCNNNNNNNGG 1 cut(s) 722
AflIII ACRYGT 1 cut(s) 277
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 2 cut(s) 388, 838
Alw26I GTCTC 1 cut(s) 788
AlwI GGATC 1 cut(s) 77
Ama87I CYCGRG 1 cut(s) 49
ApeKI GCWGC 7 cut(s) 497, 500, 539, 590, 593, 629, 787
ApoI RAATTY 1 cut(s) 688
AsiGI ACCGGT 1 cut(s) 371
Asp700I GAANNNNTTC 1 cut(s) 271
AspLEI GCGC 1 cut(s) 566
AspS9I GGNCC 1 cut(s) 883
AsuHPI GGTGA 2 cut(s) 361, 414
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 1 cut(s) 883
BbsI GAAGAC 2 cut(s) 102, 582
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 7 cut(s) 484, 487, 551, 577, 605, 616, 799
BccI CCATC 1 cut(s) 463
BceAI ACGGC 1 cut(s) 473
BclI TGATCA 1 cut(s) 151
BcoDI GTCTC 1 cut(s) 788
BfaI CTAG 1 cut(s) 659
BfmI CTRYAG 2 cut(s) 300, 591
BisI GCNGC 7 cut(s) 498, 501, 540, 591, 594, 630, 788
BlsI GCNGC 7 cut(s) 499, 502, 541, 592, 595, 631, 789
BmcAI AGTACT 1 cut(s) 319
Bme18I GGWCC 1 cut(s) 883
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 883
BmiI GGNNCC 2 cut(s) 487, 754
BmsI GCATC 2 cut(s) 71, 354
BoxI GACNNNNGTC 1 cut(s) 197
BpiI GAAGAC 2 cut(s) 102, 582
BpmI CTGGAG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 411
BsaBI GATNNNNATC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 478
BsaWI WCCGGW 2 cut(s) 371, 517
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 2 cut(s) 716, 860
Bse8I GATNNNNATC 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 478
BseGI GGATG 1 cut(s) 345
BseJI GATNNNNATC 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMII CTCAG 2 cut(s) 425, 696
BseNI ACTGG 2 cut(s) 716, 860
BseRI GAGGAG 2 cut(s) 177, 498
BseXI GCAGC 7 cut(s) 484, 487, 551, 577, 605, 616, 799
Bsh1236I CGCG 2 cut(s) 287, 564
BshTI ACCGGT 1 cut(s) 371
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 3 cut(s) 372, 393, 518
BslI CCNNNNNNNGG 1 cut(s) 722
BsmAI GTCTC 1 cut(s) 788
BsmI GAATGC 1 cut(s) 733
BsoBI CYCGRG 1 cut(s) 49
Bsp1407I TGTACA 1 cut(s) 932
Bsp143I GATC 5 cut(s) 82, 151, 209, 661, 895
Bsp68I TCGCGA 1 cut(s) 287
BspACI CCGC 4 cut(s) 503, 562, 741, 881
BspCNI CTCAG 2 cut(s) 424, 695
BspFNI CGCG 2 cut(s) 287, 564
BspLI GGNNCC 2 cut(s) 487, 754
BspMAI CTGCAG 1 cut(s) 595
BspPI GGATC 1 cut(s) 77
BsrFI RCCGGY 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 932
BsrI ACTGG 2 cut(s) 716, 860
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 1 cut(s) 478
BssMI GATC 5 cut(s) 82, 151, 209, 661, 895
BssT1I CCWWGG 1 cut(s) 478
Bst4CI ACNGT 1 cut(s) 887
Bst6I CTCTTC 1 cut(s) 121
BstAUI TGTACA 1 cut(s) 932
BstDEI CTNAG 3 cut(s) 58, 411, 682
BstF5I GGATG 1 cut(s) 345
BstFNI CGCG 2 cut(s) 287, 564
BstHHI GCGC 1 cut(s) 566
BstKTI GATC 5 cut(s) 85, 154, 212, 664, 898
BstMAI GTCTC 1 cut(s) 788
BstMBI GATC 5 cut(s) 82, 151, 209, 661, 895
BstMWI GCNNNNNNNGC 5 cut(s) 494, 563, 626, 887, 927
BstNSI RCATGY 1 cut(s) 281
BstPAI GACNNNNGTC 1 cut(s) 197
BstSFI CTRYAG 2 cut(s) 300, 591
BstUI CGCG 2 cut(s) 287, 564
BstV1I GCAGC 7 cut(s) 484, 487, 551, 577, 605, 616, 799
BstV2I GAAGAC 2 cut(s) 102, 582
BstX2I RGATCY 1 cut(s) 82
BstYI RGATCY 1 cut(s) 82
BtsCI GGATG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 810
BtuMI TCGCGA 1 cut(s) 287
CfoI GCGC 1 cut(s) 566
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 1 cut(s) 883
CpoI CGGWCCG 1 cut(s) 883
Csp6I GTAC 2 cut(s) 318, 933
CspAI ACCGGT 1 cut(s) 371
CspI CGGWCCG 1 cut(s) 883
CviAII CATG 3 cut(s) 202, 278, 616
CviQI GTAC 2 cut(s) 318, 933
DdeI CTNAG 3 cut(s) 58, 411, 682
DpnI GATC 5 cut(s) 84, 153, 211, 663, 897
DpnII GATC 5 cut(s) 82, 151, 209, 661, 895
DraIII CACNNNGTG 1 cut(s) 472
Eam1104I CTCTTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 478
Eco47I GGWCC 1 cut(s) 883
Eco57I CTGAAG 2 cut(s) 834, 840
Eco88I CYCGRG 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 478
FaeI CATG 3 cut(s) 205, 281, 619
FaiI YATR 9 cut(s) 92, 131, 203, 279, 351, 353, 527, 602, 617
FalI AAGNNNNNCTT 2 cut(s) 85, 117
FatI CATG 3 cut(s) 201, 277, 615
FauI CCCGC 1 cut(s) 734
FauNDI CATATG 1 cut(s) 351
FbaI TGATCA 1 cut(s) 151
Fnu4HI GCNGC 7 cut(s) 498, 501, 540, 591, 594, 630, 788
FokI GGATG 1 cut(s) 332
Fsp4HI GCNGC 7 cut(s) 498, 501, 540, 591, 594, 630, 788
FspBI CTAG 1 cut(s) 659
GlaI GCGC 1 cut(s) 565
GluI GCNGC 7 cut(s) 498, 501, 540, 591, 594, 630, 788
GsuI CTGGAG 1 cut(s) 291
HapII CCGG 3 cut(s) 372, 393, 518
HhaI GCGC 1 cut(s) 566
Hin1II CATG 3 cut(s) 205, 281, 619
Hin6I GCGC 1 cut(s) 564
HinP1I GCGC 1 cut(s) 564
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 5 cut(s) 5, 157, 267, 332, 778
HpaII CCGG 3 cut(s) 372, 393, 518
HphI GGTGA 2 cut(s) 361, 414
Hpy166II GTNNAC 1 cut(s) 475
Hpy188I TCNGA 4 cut(s) 156, 192, 685, 895
Hpy188III TCNNGA 3 cut(s) 51, 286, 308
Hpy8I GTNNAC 1 cut(s) 475
Hpy99I CGWCG 2 cut(s) 238, 681
HpyAV CCTTC 5 cut(s) 282, 429, 453, 792, 815
HpyCH4III ACNGT 1 cut(s) 887
HpyCH4IV ACGT 2 cut(s) 404, 471
HpyCH4V TGCA 2 cut(s) 593, 731
HpyF10VI GCNNNNNNNGC 5 cut(s) 494, 563, 626, 887, 927
HpyF3I CTNAG 3 cut(s) 58, 411, 682
HpySE526I ACGT 2 cut(s) 404, 471
Hsp92II CATG 3 cut(s) 205, 281, 619
HspAI GCGC 1 cut(s) 564
Ksp22I TGATCA 1 cut(s) 151
Kzo9I GATC 5 cut(s) 82, 151, 209, 661, 895
LmnI GCTCC 5 cut(s) 310, 485, 494, 856, 871
Lsp1109I GCAGC 7 cut(s) 484, 487, 551, 577, 605, 616, 799
LweI GCATC 2 cut(s) 71, 354
MaeI CTAG 1 cut(s) 659
MaeII ACGT 2 cut(s) 404, 471
MaeIII GTNAC 2 cut(s) 356, 744
MalI GATC 5 cut(s) 84, 153, 211, 663, 897
MboI GATC 5 cut(s) 82, 151, 209, 661, 895
MboII GAAGA 9 cut(s) 92, 93, 96, 102, 108, 117, 587, 814, 850
MflI RGATCY 1 cut(s) 82
MluCI AATT 1 cut(s) 688
MmeI TCCRAC 1 cut(s) 215
MroXI GAANNNNTTC 1 cut(s) 271
MseI TTAA 1 cut(s) 692
MslI CAYNNNNRTG 2 cut(s) 171, 350
MspA1I CMGCKG 1 cut(s) 542
MspI CCGG 3 cut(s) 372, 393, 518
Mva1269I GAATGC 1 cut(s) 733
MvnI CGCG 2 cut(s) 287, 564
MwoI GCNNNNNNNGC 5 cut(s) 494, 563, 626, 887, 927
NdeI CATATG 1 cut(s) 351
NdeII GATC 5 cut(s) 82, 151, 209, 661, 895
NlaIII CATG 3 cut(s) 205, 281, 619
NlaIV GGNNCC 2 cut(s) 487, 754
NruI TCGCGA 1 cut(s) 287
NspI RCATGY 1 cut(s) 281
PaeR7I CTCGAG 1 cut(s) 49
PciI ACATGT 1 cut(s) 277
PctI GAATGC 1 cut(s) 733
PdmI GAANNNNTTC 1 cut(s) 271
PfeI GAWTC 5 cut(s) 5, 157, 267, 332, 778
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 7 cut(s) 499, 502, 541, 592, 595, 631, 789
PscI ACATGT 1 cut(s) 277
PshAI GACNNNNGTC 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 487, 754
PspPI GGNCC 1 cut(s) 883
PsrI GAACNNNNNNTAC 2 cut(s) 283, 315
PstI CTGCAG 1 cut(s) 595
PsuI RGATCY 1 cut(s) 82
PvuII CAGCTG 1 cut(s) 542
RruI TCGCGA 1 cut(s) 287
RsaI GTAC 2 cut(s) 319, 934
RsaNI GTAC 2 cut(s) 318, 933
RseI CAYNNNNRTG 2 cut(s) 171, 350
Rsr2I CGGWCCG 1 cut(s) 883
RsrII CGGWCCG 1 cut(s) 883
SaqAI TTAA 1 cut(s) 692
SatI GCNGC 7 cut(s) 498, 501, 540, 591, 594, 630, 788
Sau3AI GATC 5 cut(s) 82, 151, 209, 661, 895
Sau96I GGNCC 1 cut(s) 883
ScaI AGTACT 1 cut(s) 319
SfaNI GCATC 2 cut(s) 71, 354
SfcI CTRYAG 2 cut(s) 300, 591
Sfr274I CTCGAG 1 cut(s) 49
SgrAI CRCCGGYG 1 cut(s) 371
SinI GGWCC 1 cut(s) 883
SlaI CTCGAG 1 cut(s) 49
SmiMI CAYNNNNRTG 2 cut(s) 171, 350
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
Sse9I AATT 1 cut(s) 688
SsiI CCGC 4 cut(s) 503, 562, 741, 881
SspMI CTAG 1 cut(s) 659
StyI CCWWGG 1 cut(s) 478
TaaI ACNGT 1 cut(s) 887
TaiI ACGT 2 cut(s) 407, 474
TaqI TCGA 1 cut(s) 50
TasI AATT 1 cut(s) 688
TatI WGTACW 2 cut(s) 317, 932
TfiI GAWTC 5 cut(s) 5, 157, 267, 332, 778
Tru1I TTAA 1 cut(s) 692
Tru9I TTAA 1 cut(s) 692
TscAI CASTG 1 cut(s) 817
TseI GCWGC 7 cut(s) 497, 500, 539, 590, 593, 629, 787
TspDTI ATGAA 2 cut(s) 345, 639
TspRI CASTG 1 cut(s) 817
VpaK11BI GGWCC 1 cut(s) 883
XapI RAATTY 1 cut(s) 688
XceI RCATGY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 271
XspI CTAG 1 cut(s) 659
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.