Rmu_co8215104.1_g000001

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8215104.1
OG0010088
Physical Location & Seq
Reverse (-)
1 .. 731
731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8215104.1_g000001.1.cds

Sequence Viewer

Length: 713 bp
gtagctacttcggaaaatggtcttgcaacgactccgtcccctgctggctctcttctaagtacaagctacatcgatgaggattttcaaggcggctcgacacttaagactgagcagcttaatggtgagttgagccagggcaatgattcagcaacacaatcgcctcctaagcaggtgatggagcggcctagtgagccgagtactccgtacaggatcccggattatgtctttgcgaggaaaccatccacagccccgatggaatggagtgctgcttccaatgaatccctattcagcattcagatgggtaacatgagtttcaccagagaacagtttgcctttttaggtaaatctggtgagttgggtttatctggagaacagaacatgttgtccggtccatatatagacttctcgagtaaccagcctccggataagcaagttgaattttcccaaaacggcggcaatttggatgagggcccaaatccgagagtaactgaagcaaaagccgtagagactatgagagaggttataagggaaattgccgaaaatgacaacaaagcaagtccatcatcttcggcggataatcacagcaaaggaagcccgtcgcccagcgtccgaaatcctatagtcagcccatccctcactgaacacaagtttcataacatatctagcctttctcatgcttctgatggaagtactaaatcatttgcatttccagt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

238

Amino Acids

25.51

Weight (kDa)

4.81

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AarI CACCTGC 1 cut(s) 160
Acc36I ACCTGC 1 cut(s) 160
AccBSI CCGCTC 1 cut(s) 181
AccIII TCCGGA 1 cut(s) 421
AciI CCGC 4 cut(s) 90, 181, 453, 572
AclWI GGATC 2 cut(s) 205, 218
AcsI RAATTY 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 510
AfaI GTAC 4 cut(s) 61, 199, 206, 691
AfiI CCNNNNNNNGG 1 cut(s) 421
AflII CTTAAG 1 cut(s) 101
AflIII ACRYGT 1 cut(s) 378
AgsI TTSAA 2 cut(s) 86, 437
AjnI CCWGG 1 cut(s) 132
AloI GAACNNNNNNTCC 2 cut(s) 368, 400
AluBI AGCT 3 cut(s) 5, 66, 115
AluI AGCT 3 cut(s) 5, 66, 115
Alw26I GTCTC 1 cut(s) 500
AlwI GGATC 2 cut(s) 205, 218
Ama87I CYCGRG 1 cut(s) 406
Aor13HI TCCGGA 1 cut(s) 421
AoxI GGCC 2 cut(s) 182, 469
ApaI GGGCCC 1 cut(s) 473
ApeKI GCWGC 2 cut(s) 112, 266
ApoI RAATTY 1 cut(s) 437
AspS9I GGNCC 3 cut(s) 389, 469, 470
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 4 cut(s) 134, 184, 307, 362
AvaI CYCGRG 1 cut(s) 406
AvaII GGWCC 1 cut(s) 389
BaeGI GKGCMC 1 cut(s) 473
BamHI GGATCC 1 cut(s) 210
BanII GRGCYC 1 cut(s) 473
BbvI GCAGC 2 cut(s) 124, 253
BccI CCATC 7 cut(s) 169, 247, 247, 292, 568, 637, 677
BceAI ACGGC 2 cut(s) 466, 485
BcgI CGANNNNNNTGC 2 cut(s) 138, 172
BciT130I CCWGG 1 cut(s) 134
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 1 cut(s) 500
BfaI CTAG 2 cut(s) 186, 663
BfmI CTRYAG 1 cut(s) 618
BfrI CTTAAG 1 cut(s) 101
BfuAI ACCTGC 1 cut(s) 160
BisI GCNGC 5 cut(s) 91, 113, 182, 267, 454
BlsI GCNGC 5 cut(s) 92, 114, 183, 268, 455
BmcAI AGTACT 2 cut(s) 199, 691
Bme1390I CCNGG 2 cut(s) 134, 215
Bme18I GGWCC 1 cut(s) 389
BmeT110I CYCGRG 1 cut(s) 406
BmgT120I GGNCC 3 cut(s) 389, 469, 470
BmiI GGNNCC 2 cut(s) 212, 471
BmrFI CCNGG 2 cut(s) 134, 215
BpmI CTGGAG 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 215
Bsa29I ATCGAT 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 133
BsaWI WCCGGW 2 cut(s) 386, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 421
Bse1I ACTGG 1 cut(s) 710
Bse3DI GCAATG 1 cut(s) 145
BseAI TCCGGA 1 cut(s) 421
BseBI CCWGG 1 cut(s) 134
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 133
BseGI GGATG 3 cut(s) 239, 469, 629
BseLI CCNNNNNNNGG 1 cut(s) 421
BseMI GCAATG 1 cut(s) 145
BseMII CTCAG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 710
BseSI GKGCMC 1 cut(s) 473
BseXI GCAGC 2 cut(s) 124, 253
BseYI CCCAGC 1 cut(s) 602
BshFI GGCC 2 cut(s) 184, 471
BshVI ATCGAT 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 3 cut(s) 215, 387, 422
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 421
BsmAI GTCTC 1 cut(s) 500
BsmFI GGGAC 1 cut(s) 22
BsmI GAATGC 1 cut(s) 291
BsnI GGCC 2 cut(s) 184, 471
BsoBI CYCGRG 1 cut(s) 406
Bsp120I GGGCCC 1 cut(s) 469
Bsp1286I GDGCHC 1 cut(s) 473
Bsp13I TCCGGA 1 cut(s) 421
Bsp143I GATC 1 cut(s) 210
BspACI CCGC 4 cut(s) 90, 181, 453, 572
BspANI GGCC 2 cut(s) 184, 471
BspCNI CTCAG 1 cut(s) 100
BspDI ATCGAT 1 cut(s) 72
BspEI TCCGGA 1 cut(s) 421
BspLI GGNNCC 2 cut(s) 212, 471
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 2 cut(s) 205, 218
BspTI CTTAAG 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 181
BsrDI GCAATG 1 cut(s) 145
BsrI ACTGG 1 cut(s) 710
BssECI CCNNGG 1 cut(s) 133
BssMI GATC 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 1 cut(s) 327
Bst6I CTCTTC 1 cut(s) 57
BstAFI CTTAAG 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 3 cut(s) 56, 108, 165
BstF5I GGATG 3 cut(s) 239, 469, 629
BstKTI GATC 1 cut(s) 213
BstMAI GTCTC 1 cut(s) 500
BstMBI GATC 1 cut(s) 210
BstMWI GCNNNNNNNGC 3 cut(s) 166, 190, 591
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 1 cut(s) 382
BstSCI CCNGG 2 cut(s) 132, 213
BstSFI CTRYAG 1 cut(s) 618
BstSLI GKGCMC 1 cut(s) 473
BstV1I GCAGC 2 cut(s) 124, 253
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
Bsu15I ATCGAT 1 cut(s) 72
BsuRI GGCC 2 cut(s) 184, 471
BsuTUI ATCGAT 1 cut(s) 72
BtsCI GGATG 3 cut(s) 239, 469, 629
BtsIMutI CAGTG 1 cut(s) 636
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 46
Cfr13I GGNCC 3 cut(s) 389, 469, 470
ClaI ATCGAT 1 cut(s) 72
CseI GACGC 1 cut(s) 595
Csp6I GTAC 4 cut(s) 60, 198, 205, 690
CviAII CATG 3 cut(s) 307, 379, 674
CviQI GTAC 4 cut(s) 60, 198, 205, 690
DdeI CTNAG 3 cut(s) 56, 108, 165
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 57
EarI CTCTTC 1 cut(s) 57
EciI GGCGGA 1 cut(s) 587
Eco24I GRGCYC 1 cut(s) 473
Eco47I GGWCC 1 cut(s) 389
Eco57I CTGAAG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 406
EcoO109I RGGNCCY 1 cut(s) 469
EcoRII CCWGG 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 473
FaeI CATG 3 cut(s) 310, 382, 677
FaqI GGGAC 1 cut(s) 22
FatI CATG 3 cut(s) 306, 378, 673
Fnu4HI GCNGC 5 cut(s) 91, 113, 182, 267, 454
FokI GGATG 3 cut(s) 226, 476, 616
FriOI GRGCYC 1 cut(s) 473
Fsp4HI GCNGC 5 cut(s) 91, 113, 182, 267, 454
FspBI CTAG 2 cut(s) 186, 663
GluI GCNGC 5 cut(s) 91, 113, 182, 267, 454
GsaI CCCAGC 1 cut(s) 606
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 2 cut(s) 184, 471
HapII CCGG 3 cut(s) 215, 387, 422
HgaI GACGC 1 cut(s) 595
Hin1II CATG 3 cut(s) 310, 382, 677
HinfI GANTC 3 cut(s) 31, 143, 278
HpaII CCGG 3 cut(s) 215, 387, 422
HphI GGTGA 4 cut(s) 134, 184, 307, 362
Hpy188I TCNGA 5 cut(s) 13, 297, 480, 611, 682
Hpy188III TCNNGA 3 cut(s) 366, 406, 422
Hpy99I CGWCG 1 cut(s) 601
HpyCH4III ACNGT 1 cut(s) 327
HpyCH4V TGCA 2 cut(s) 26, 704
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 190, 591
HpyF3I CTNAG 3 cut(s) 56, 108, 165
Hsp92II CATG 3 cut(s) 310, 382, 677
Kpn2I TCCGGA 1 cut(s) 421
Kzo9I GATC 1 cut(s) 210
LmnI GCTCC 1 cut(s) 178
Lsp1109I GCAGC 2 cut(s) 124, 253
MaeI CTAG 2 cut(s) 186, 663
MaeIII GTNAC 3 cut(s) 302, 410, 484
MalI GATC 1 cut(s) 212
MbiI CCGCTC 1 cut(s) 181
MboI GATC 1 cut(s) 210
MboII GAAGA 2 cut(s) 44, 558
MflI RGATCY 1 cut(s) 210
MhlI GDGCHC 1 cut(s) 473
MluCI AATT 3 cut(s) 437, 457, 531
MlyI GAGTC 1 cut(s) 25
MnlI CCTC 7 cut(s) 70, 171, 225, 429, 460, 511, 644
MroI TCCGGA 1 cut(s) 421
MseI TTAA 2 cut(s) 102, 117
MslI CAYNNNNRTG 1 cut(s) 296
MspCI CTTAAG 1 cut(s) 101
MspI CCGG 3 cut(s) 215, 387, 422
MspR9I CCNGG 2 cut(s) 134, 215
Mva1269I GAATGC 1 cut(s) 291
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 3 cut(s) 166, 190, 591
NciI CCSGG 1 cut(s) 215
NdeII GATC 1 cut(s) 210
NlaIII CATG 3 cut(s) 310, 382, 677
NlaIV GGNNCC 2 cut(s) 212, 471
NmeAIII GCCGAG 1 cut(s) 219
NspI RCATGY 1 cut(s) 382
PaeR7I CTCGAG 1 cut(s) 406
PaqCI CACCTGC 1 cut(s) 160
PciI ACATGT 1 cut(s) 378
PctI GAATGC 1 cut(s) 291
PfeI GAWTC 2 cut(s) 143, 278
PflFI GACNNNGTC 1 cut(s) 34
PfoI TCCNGGA 1 cut(s) 213
PkrI GCNGC 5 cut(s) 92, 114, 183, 268, 455
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
PscI ACATGT 1 cut(s) 378
PsiI TTATAA 1 cut(s) 524
Psp6I CCWGG 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 602
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 2 cut(s) 212, 471
PspOMI GGGCCC 1 cut(s) 469
PspPI GGNCC 3 cut(s) 389, 469, 470
PsuI RGATCY 1 cut(s) 210
PsyI GACNNNGTC 1 cut(s) 34
RsaI GTAC 4 cut(s) 61, 199, 206, 691
RsaNI GTAC 4 cut(s) 60, 198, 205, 690
RseI CAYNNNNRTG 1 cut(s) 296
SaqAI TTAA 2 cut(s) 102, 117
SatI GCNGC 5 cut(s) 91, 113, 182, 267, 454
Sau3AI GATC 1 cut(s) 210
Sau96I GGNCC 3 cut(s) 389, 469, 470
ScaI AGTACT 2 cut(s) 199, 691
SchI GAGTC 1 cut(s) 25
ScrFI CCNGG 2 cut(s) 134, 215
SduI GDGCHC 1 cut(s) 473
SetI ASST 6 cut(s) 7, 68, 117, 174, 343, 522
SfcI CTRYAG 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 406
SinI GGWCC 1 cut(s) 389
SlaI CTCGAG 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 296
SmlI CTYRAG 2 cut(s) 101, 406
SmoI CTYRAG 2 cut(s) 101, 406
Sse9I AATT 3 cut(s) 437, 457, 531
SsiI CCGC 4 cut(s) 90, 181, 453, 572
SspMI CTAG 2 cut(s) 186, 663
StyD4I CCNGG 2 cut(s) 132, 213
TaaI ACNGT 1 cut(s) 327
TaqI TCGA 3 cut(s) 72, 95, 407
TasI AATT 3 cut(s) 437, 457, 531
TatI WGTACW 3 cut(s) 59, 197, 689
TauI GCSGC 3 cut(s) 93, 184, 456
TfiI GAWTC 2 cut(s) 143, 278
Tru1I TTAA 2 cut(s) 102, 117
Tru9I TTAA 2 cut(s) 102, 117
TscAI CASTG 1 cut(s) 643
TseI GCWGC 2 cut(s) 112, 266
TspDTI ATGAA 2 cut(s) 291, 641
TspGWI ACGGA 2 cut(s) 24, 192
TspRI CASTG 1 cut(s) 643
Tth111I GACNNNGTC 1 cut(s) 34
Vha464I CTTAAG 1 cut(s) 101
VpaK11BI GGWCC 1 cut(s) 389
XapI RAATTY 1 cut(s) 437
XceI RCATGY 1 cut(s) 382
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 406
XspI CTAG 2 cut(s) 186, 663
ZrmI AGTACT 2 cut(s) 199, 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.