AT1G74450

BEST Arabidopsis thaliana protein match is Protein of

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010852
Physical Location & Seq
Forward (+)
27982453 .. 27984280
1828 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74450.1

Sequence Viewer

Length: 1194 bp
ATGCCAGCAACGGAATATCAAAGATCATTCGGGAGGTCTTTTCTGAATTTACGGCGAGACACGGCGGTGAACTCGGTTGAGTCAACGACGGTTACCCCCGAGTTGACTCAGATGGAAGCAGAGCTTGTTTCGTTTCAGAGAAAAGTTGCTGAGAGGTTTATCGATCTCAATGCGTCTAGTTGTGAAGATCTACTGTCTCTCGAATGGGTTGGGAAGCTTCTTGATTCGTTTCTATCTTGTCAAGAAGAGTTTCGTTCCATAGTCATCAATCATCGTTCGATGATTACTAAACCACCGATGGATCGATTGGTTTCAGATTACTTCGAGAGAAGTGTTAAAGCTCTTGATGTTTGTAACGCGATTCGTGATGGTGTTGAACAGATCCGTCAATGGCAGAAACTTATCGAGATTGTAATTTGTGCTTTCAACAACAATGGAGGAGGAAGTAGTGGCAAGAGACCTCTTGGAGAAGGTCAGTTTCGTCGTGCGAGGAAGACTTTGATTGAGTTAGCTATTGGTATGCTTGATGAGAAGGATTCATCTTCTAGCTCAGTGTCGTCTCAGCATCGTAACCGGTCTTTTGGAAGGAACAAGGAGCAGCTTCATCACCGAACAATCGGGCATTTCAGGTCGCTTTCTTGGAGTGTTTCGAGATCATGGTCTGCTTCTAAGCAACTACAAGCTATTGGAAACAACTTAGCTACGCCTCGAGCTAGTGACATTACTGCAACTAATGGTCTTATTGTTCCGGTTTATACAATGACTACGGTATTGTTGTTTGTGATGTGGGCACTTGTAGCTGCGATTCCTTGTCAAGACAGAGGTTTGCAAGTACATTTCAATGTTCCTAGGAATTACCAATGGGGAGGTTCGTTGATGTCGTTGCACGATAGGATCATTGAGGAATCGAAGAAGAGAGAGAGGAAGAATACATGTGGGTTGTTGAAAGAGATTCATCAGTTTGAGAAGACTTCAAGATTGATGAATGAGTTGGTTGATTCTGTTCAGTTTCCTTTGTCTGAAGAGAAAGAGATGGAAGTGAGAGAGAGAGTTGAGGAATTGGGGAAGCTTCAAGAGGCTTTGAAGAATGGTTTGGATCCGTTTGAGAGGAAAGTGAGGGAAGTGTTTCATCGGATTGTAAGAAGCAGAACAGAAGGTCTTGATACTGTTGGAAAGCATCATGGTTCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

397

Amino Acids

45.32

Weight (kDa)

8.84

Isoelectric Point (pI)

46.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 1 - 390 1.5e-184 Protein ROH1-like
DUF677 PF05055 64 - 169 3.9e-07 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18740 AT1G74450
fragaria_vesca FvH4_2g13680 FvH4_2g13680
malus_domestica MD05G1026700.v1.1 MD10G1025900.v1.1
prunus_persica Prupe.8G031200_v2.0.a1
pyrus_communis pycom05g01850 pycom10g01740
rosa_chinensis RchiOBHm_Chr6g0275361
rosa_laevigata RLG00000013471
rosa_multiflora Rmu_co8417379.1_g000001 Rmu_sc0004143.1_g000001 Rmu_sc0019329.1_g000002
rosa_roxburghii Rroxscaffold_7G00193380
rosa_rugosa Rorug06G0091100
rosa_samantha Rh6AG205000 Rh6CG210200 Rh6DG200500
rosa_wichuraiana Rw6G017790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 359
AciI CCGC 1 cut(s) 65
AclWI GGATC 5 cut(s) 309, 376, 902, 1091, 1104
AcsI RAATTY 1 cut(s) 46
AcuI CTGAAG 1 cut(s) 1041
AfaI GTAC 1 cut(s) 834
AflIII ACRYGT 1 cut(s) 932
AgeI ACCGGT 1 cut(s) 573
AgsI TTSAA 7 cut(s) 377, 427, 841, 946, 975, 1073, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 1076, 1108
AleI CACNNNNGTG 1 cut(s) 65
Alw26I GTCTC 4 cut(s) 51, 201, 451, 564
AlwI GGATC 5 cut(s) 309, 376, 902, 1091, 1104
Ama87I CYCGRG 2 cut(s) 98, 708
ApeKI GCWGC 2 cut(s) 598, 800
ApoI RAATTY 1 cut(s) 46
AsiGI ACCGGT 1 cut(s) 573
Asp700I GAANNNNTTC 2 cut(s) 249, 1125
AspA2I CCTAGG 1 cut(s) 848
AsuHPI GGTGA 2 cut(s) 79, 599
AvaI CYCGRG 2 cut(s) 98, 708
AvrII CCTAGG 1 cut(s) 848
BaeGI GKGCMC 1 cut(s) 793
BamHI GGATCC 1 cut(s) 1096
BbsI GAAGAC 2 cut(s) 500, 974
BbvI GCAGC 2 cut(s) 610, 787
BccI CCATC 4 cut(s) 106, 292, 362, 1027
BceAI ACGGC 2 cut(s) 68, 78
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BcoDI GTCTC 4 cut(s) 51, 201, 451, 564
BfaI CTAG 4 cut(s) 177, 546, 714, 849
BglII AGATCT 1 cut(s) 187
BisI GCNGC 2 cut(s) 599, 801
BlnI CCTAGG 1 cut(s) 848
BlsI GCNGC 2 cut(s) 600, 802
BmeT110I CYCGRG 2 cut(s) 98, 708
BmiI GGNNCC 1 cut(s) 1098
BmsI GCATC 2 cut(s) 574, 1186
BpiI GAAGAC 2 cut(s) 500, 974
Bsa29I ATCGAT 2 cut(s) 162, 304
BsaI GGTCTC 1 cut(s) 451
BsaJI CCNNGG 1 cut(s) 848
BsaWI WCCGGW 2 cut(s) 573, 748
Bse118I RCCGGY 1 cut(s) 573
BseCI ATCGAT 2 cut(s) 162, 304
BseDI CCNNGG 1 cut(s) 848
BseMII CTCAG 4 cut(s) 122, 141, 564, 575
BseRI GAGGAG 1 cut(s) 453
BseSI GKGCMC 1 cut(s) 793
BseXI GCAGC 2 cut(s) 610, 787
Bsh1236I CGCG 1 cut(s) 359
BshTI ACCGGT 1 cut(s) 573
BshVI ATCGAT 2 cut(s) 162, 304
BsiHKCI CYCGRG 2 cut(s) 98, 708
BsiSI CCGG 2 cut(s) 574, 749
BsmAI GTCTC 4 cut(s) 51, 201, 451, 564
BsmBI CGTCTC 1 cut(s) 564
Bso31I GGTCTC 1 cut(s) 451
BsoBI CYCGRG 2 cut(s) 98, 708
Bsp1286I GDGCHC 1 cut(s) 793
Bsp143I GATC 8 cut(s) 23, 163, 187, 301, 381, 653, 894, 1096
BspACI CCGC 1 cut(s) 65
BspCNI CTCAG 4 cut(s) 121, 142, 563, 574
BspDI ATCGAT 2 cut(s) 162, 304
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 1 cut(s) 1098
BspPI GGATC 5 cut(s) 309, 376, 902, 1091, 1104
BspTNI GGTCTC 1 cut(s) 451
BsrFI RCCGGY 1 cut(s) 573
BssAI RCCGGY 1 cut(s) 573
BssECI CCNNGG 1 cut(s) 848
BssMI GATC 8 cut(s) 23, 163, 187, 301, 381, 653, 894, 1096
BssT1I CCWWGG 1 cut(s) 848
Bst4CI ACNGT 4 cut(s) 91, 195, 769, 1168
Bst6I CTCTTC 3 cut(s) 240, 908, 1017
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 6 cut(s) 108, 150, 550, 561, 669, 697
BstEII GGTNACC 1 cut(s) 91
BstFNI CGCG 1 cut(s) 359
BstKTI GATC 8 cut(s) 26, 166, 190, 304, 384, 656, 897, 1099
BstMAI GTCTC 4 cut(s) 51, 201, 451, 564
BstMBI GATC 8 cut(s) 23, 163, 187, 301, 381, 653, 894, 1096
BstMWI GCNNNNNNNGC 1 cut(s) 797
BstNSI RCATGY 1 cut(s) 936
BstPI GGTNACC 1 cut(s) 91
BstSLI GKGCMC 1 cut(s) 793
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 2 cut(s) 610, 787
BstV2I GAAGAC 2 cut(s) 500, 974
BstX2I RGATCY 3 cut(s) 187, 381, 1096
BstYI RGATCY 3 cut(s) 187, 381, 1096
Bsu15I ATCGAT 2 cut(s) 162, 304
BsuTUI ATCGAT 2 cut(s) 162, 304
BtsIMutI CAGTG 1 cut(s) 558
Cac8I GCNNGC 1 cut(s) 6
Cfr10I RCCGGY 1 cut(s) 573
ClaI ATCGAT 2 cut(s) 162, 304
CseI GACGC 1 cut(s) 162
Csp6I GTAC 1 cut(s) 833
CspAI ACCGGT 1 cut(s) 573
CviAII CATG 3 cut(s) 657, 933, 1181
CviQI GTAC 1 cut(s) 833
DdeI CTNAG 6 cut(s) 108, 150, 550, 561, 669, 697
DpnI GATC 8 cut(s) 25, 165, 189, 303, 383, 655, 896, 1098
DpnII GATC 8 cut(s) 23, 163, 187, 301, 381, 653, 894, 1096
Eam1104I CTCTTC 3 cut(s) 240, 908, 1017
EarI CTCTTC 3 cut(s) 240, 908, 1017
Eco130I CCWWGG 1 cut(s) 848
Eco31I GGTCTC 1 cut(s) 451
Eco57I CTGAAG 1 cut(s) 1041
Eco88I CYCGRG 2 cut(s) 98, 708
Eco91I GGTNACC 1 cut(s) 91
EcoO65I GGTNACC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 848
ErhI CCWWGG 1 cut(s) 848
Esp3I CGTCTC 1 cut(s) 564
FaeI CATG 3 cut(s) 660, 936, 1184
FaiI YATR 6 cut(s) 260, 521, 658, 756, 934, 1182
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FatI CATG 3 cut(s) 656, 932, 1180
Fnu4HI GCNGC 2 cut(s) 599, 801
Fsp4HI GCNGC 2 cut(s) 599, 801
FspBI CTAG 4 cut(s) 177, 546, 714, 849
GluI GCNGC 2 cut(s) 599, 801
HapII CCGG 2 cut(s) 574, 749
HgaI GACGC 1 cut(s) 162
Hin1II CATG 3 cut(s) 660, 936, 1184
HincII GTYRAC 2 cut(s) 84, 105
HindII GTYRAC 2 cut(s) 84, 105
HindIII AAGCTT 2 cut(s) 215, 1067
HinfI GANTC 9 cut(s) 80, 106, 224, 361, 536, 805, 905, 952, 998
HpaII CCGG 2 cut(s) 574, 749
HphI GGTGA 2 cut(s) 79, 599
Hpy166II GTNNAC 3 cut(s) 70, 84, 105
Hpy188I TCNGA 7 cut(s) 45, 111, 138, 316, 1021, 1134, 1189
Hpy8I GTNNAC 3 cut(s) 70, 84, 105
Hpy99I CGWCG 2 cut(s) 91, 486
HpyAV CCTTC 4 cut(s) 464, 526, 579, 1148
HpyCH4III ACNGT 4 cut(s) 91, 195, 769, 1168
HpyCH4V TGCA 3 cut(s) 728, 829, 886
HpyF10VI GCNNNNNNNGC 1 cut(s) 797
HpyF3I CTNAG 6 cut(s) 108, 150, 550, 561, 669, 697
Hsp92II CATG 3 cut(s) 660, 936, 1184
Kzo9I GATC 8 cut(s) 23, 163, 187, 301, 381, 653, 894, 1096
LmnI GCTCC 1 cut(s) 595
LpnPI CCDG 4 cut(s) 18, 587, 613, 762
Lsp1109I GCAGC 2 cut(s) 610, 787
LweI GCATC 2 cut(s) 574, 1186
MaeI CTAG 4 cut(s) 177, 546, 714, 849
MaeIII GTNAC 4 cut(s) 91, 353, 569, 716
MalI GATC 8 cut(s) 25, 165, 189, 303, 383, 655, 896, 1098
MboI GATC 8 cut(s) 23, 163, 187, 301, 381, 653, 894, 1096
MflI RGATCY 3 cut(s) 187, 381, 1096
MhlI GDGCHC 1 cut(s) 793
MluCI AATT 4 cut(s) 46, 414, 853, 1058
MlyI GAGTC 2 cut(s) 89, 100
MmeI TCCRAC 1 cut(s) 1150
MroXI GAANNNNTTC 2 cut(s) 249, 1125
MseI TTAA 1 cut(s) 336
MslI CAYNNNNRTG 2 cut(s) 65, 840
MspI CCGG 2 cut(s) 574, 749
MvnI CGCG 1 cut(s) 359
MwoI GCNNNNNNNGC 1 cut(s) 797
NdeII GATC 8 cut(s) 23, 163, 187, 301, 381, 653, 894, 1096
NlaIII CATG 3 cut(s) 660, 936, 1184
NlaIV GGNNCC 1 cut(s) 1098
NmuCI GTSAC 1 cut(s) 716
NspI RCATGY 1 cut(s) 936
OliI CACNNNNGTG 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 708
PciI ACATGT 1 cut(s) 932
PcsI WCGNNNNNNNCGW 1 cut(s) 878
PdmI GAANNNNTTC 2 cut(s) 249, 1125
PfeI GAWTC 7 cut(s) 224, 361, 536, 805, 905, 952, 998
PinAI ACCGGT 1 cut(s) 573
PkrI GCNGC 2 cut(s) 600, 802
PleI GAGTC 2 cut(s) 88, 100
PpsI GAGTC 2 cut(s) 88, 100
PscI ACATGT 1 cut(s) 932
PspEI GGTNACC 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 1098
PspXI VCTCGAGB 1 cut(s) 708
PsuI RGATCY 3 cut(s) 187, 381, 1096
RsaI GTAC 1 cut(s) 834
RsaNI GTAC 1 cut(s) 833
RseI CAYNNNNRTG 2 cut(s) 65, 840
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 2 cut(s) 599, 801
Sau3AI GATC 8 cut(s) 23, 163, 187, 301, 381, 653, 894, 1096
SchI GAGTC 2 cut(s) 89, 100
SduI GDGCHC 1 cut(s) 793
SfaNI GCATC 2 cut(s) 574, 1186
Sfr274I CTCGAG 1 cut(s) 708
SlaI CTCGAG 1 cut(s) 708
SmiMI CAYNNNNRTG 2 cut(s) 65, 840
SmlI CTYRAG 1 cut(s) 708
SmoI CTYRAG 1 cut(s) 708
Sse9I AATT 4 cut(s) 46, 414, 853, 1058
SsiI CCGC 1 cut(s) 65
SspMI CTAG 4 cut(s) 177, 546, 714, 849
StyI CCWWGG 1 cut(s) 848
TaaI ACNGT 4 cut(s) 91, 195, 769, 1168
TaqI TCGA 9 cut(s) 162, 201, 278, 304, 324, 405, 650, 709, 908
TasI AATT 4 cut(s) 46, 414, 853, 1058
TatI WGTACW 1 cut(s) 832
TfiI GAWTC 7 cut(s) 224, 361, 536, 805, 905, 952, 998
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 1 cut(s) 558
TseFI GTSAC 1 cut(s) 716
TseI GCWGC 2 cut(s) 598, 800
Tsp45I GTSAC 1 cut(s) 716
TspDTI ATGAA 5 cut(s) 528, 593, 944, 998, 1118
TspGWI ACGGA 3 cut(s) 26, 374, 1089
TspRI CASTG 1 cut(s) 558
XapI RAATTY 1 cut(s) 46
XceI RCATGY 1 cut(s) 936
XhoI CTCGAG 1 cut(s) 708
XmaJI CCTAGG 1 cut(s) 848
XmnI GAANNNNTTC 2 cut(s) 249, 1125
XspI CTAG 4 cut(s) 177, 546, 714, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.