Rh6AG205000

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0010852
Physical Location & Seq
Reverse (-)
36598858 .. 36602334
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG205000.1

Sequence Viewer

Length: 1197 bp
ATGCCCGCGACGGAATTCCAAAGGTCGTCGGCAGCTGGGTTCAACCAATTCGGGCGCTCCTCCGTTTTCAGCCTCAGACGCCGCCCCCACAACCACCACCACCACCACCACAACCTCGTCAATCCAGTCATGGACGGCGCCAAAGCTCTCGACCTCGACACCTTCCAGACCCACGTCGCCGATTGCTTCCAACACCTCTCCTCCCCCTCCCAAGACCTCCTCTCCCTCTCCTGGCTCCGCGACCTCCTCGACGGCTTCCTCCGCATTCACCACCAGTTCAAGCTCCTCCTCCTCTCCACCCCAAAATCCAACGTCAGCAGGCCCGCCGTCGACCGCCTCCTCTCCGATTACTTCGAGCGCAGCGTCAAGGCGCTCGACGTCTGCAATGCGATTCGCGACGGGATTGAGCAGATGAGGCAGTGGCAGAAGCTGTTGGAGATTGTGCTCTGCGCTCTCGACCGGGACCGGACTCTCGGCGAAGGCCAGTTCCGGCGAGCCAAGAAGGCCCTGATAGATTTGGCTATTGCAATGCTAGACGACAAGGACAGCTCCAACAACACCACCCTCGCCCACCGCAACCGGTCCTTTGGCCGGAGCAACGCCCCGGCCAAAGACTACCGGTCGATGGGCCACTTCCGATCTCTGTCGTGGAGCGTCTCGCGGTCGTGGTCGGCGGCGAGGCAGCTACAGGCGATTGGGAACAACCTCTGCGCGCCGAGGCCCAATGAAGTCATGGCGACCAATGGGCTGGCGTCGGCTGTGTTCACCATCAATTCTGTTCTACTGTTCGTAATGTTGGCGCTGGTGGCGGCGATTCCGTGCCAGGACCGCGGCCTCCAGGTCCACTTCACGATTCCGAGGACCTTCGGGTGGGCGGCGCCGCTGGTTTCGCTGCACGAGAGGATAATGGAGGAGTCGAAGCGGCGGGACCGGAGGAACGCCTGTGCGCTTTTGAAGGAGATTCATCAGATCGAGAAATGTTCCCGGGTCATAAGCGAATTGGCTGATTCGGTTCAGTTTCCGTTGAGTGAGGAGAAAGAAGGGGAGGTCAGGCAGAGAGTTCAGGAGCTTTCGCAGCTCTGCGAGGGGATAAAGGAGGGGTTGGACCCTCTGGAAAGACAGGTCAGGGAGGTCTTCCATGGAATTGTGCGGAGCAGAACAGAGGGGATGGACTCTTTGGGAAAGACCAATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

398

Amino Acids

45.11

Weight (kDa)

9.05

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 12 - 393 5.7e-146 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18740 AT1G74450
fragaria_vesca FvH4_2g13680 FvH4_2g13680
malus_domestica MD05G1026700.v1.1 MD10G1025900.v1.1
prunus_persica Prupe.8G031200_v2.0.a1
pyrus_communis pycom05g01850 pycom10g01740
rosa_chinensis RchiOBHm_Chr6g0275361
rosa_laevigata RLG00000013471
rosa_multiflora Rmu_co8417379.1_g000001 Rmu_sc0004143.1_g000001 Rmu_sc0019329.1_g000002
rosa_roxburghii Rroxscaffold_7G00193380
rosa_rugosa Rorug06G0091100
rosa_samantha Rh6AG205000 Rh6CG210200 Rh6DG200500
rosa_wichuraiana Rw6G017790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 381
AccB1I GGYRCC 2 cut(s) 137, 877
AccI GTMKAC 1 cut(s) 330
AccII CGCG 6 cut(s) 8, 240, 396, 661, 713, 831
AcoI YGGCCR 2 cut(s) 589, 606
AcsI RAATTY 1 cut(s) 14
AcyI GRCGYC 5 cut(s) 79, 138, 378, 752, 878
AfiI CCNNNNNNNGG 4 cut(s) 231, 591, 625, 870
AgeI ACCGGT 2 cut(s) 579, 618
AgsI TTSAA 3 cut(s) 43, 280, 955
AhdI GACNNNNNGTC 1 cut(s) 619
AjiI CACGTC 1 cut(s) 175
AjnI CCWGG 3 cut(s) 230, 822, 837
AluBI AGCT 8 cut(s) 35, 146, 283, 430, 549, 685, 1069, 1078
AluI AGCT 8 cut(s) 35, 146, 283, 430, 549, 685, 1069, 1078
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 1 cut(s) 661
AlwNI CAGNNNCTG 1 cut(s) 430
Ama87I CYCGRG 1 cut(s) 984
AoxI GGCC 8 cut(s) 320, 481, 504, 589, 606, 628, 719, 832
ApeKI GCWGC 5 cut(s) 32, 360, 682, 892, 1075
ApoI RAATTY 1 cut(s) 14
AsiGI ACCGGT 2 cut(s) 579, 618
AsuC2I CCSGG 4 cut(s) 461, 605, 985, 986
AsuHPI GGTGA 2 cut(s) 260, 757
AvaI CYCGRG 1 cut(s) 984
AvaII GGWCC 7 cut(s) 463, 582, 826, 841, 861, 928, 1105
BanI GGYRCC 2 cut(s) 137, 877
BauI CACGAG 1 cut(s) 896
BbsI GAAGAC 1 cut(s) 1126
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 5 cut(s) 44, 372, 694, 879, 1087
BccI CCATC 3 cut(s) 619, 776, 1162
BceAI ACGGC 3 cut(s) 151, 268, 311
BciT130I CCWGG 3 cut(s) 232, 824, 839
BcnI CCSGG 4 cut(s) 461, 605, 985, 986
BcoDI GTCTC 1 cut(s) 661
BfaI CTAG 1 cut(s) 533
BfmI CTRYAG 1 cut(s) 686
BfoI RGCGCY 5 cut(s) 58, 141, 374, 803, 881
BglI GCCNNNNNGGC 1 cut(s) 503
Bme1390I CCNGG 7 cut(s) 232, 461, 605, 824, 839, 985, 986
Bme18I GGWCC 7 cut(s) 463, 582, 826, 841, 861, 928, 1105
BmeRI GACNNNNNGTC 1 cut(s) 619
BmeT110I CYCGRG 1 cut(s) 984
BmgBI CACGTC 1 cut(s) 175
BmiI GGNNCC 6 cut(s) 139, 236, 464, 879, 929, 1107
BmrFI CCNGG 7 cut(s) 232, 461, 605, 824, 839, 985, 986
BoxI GACNNNNGTC 1 cut(s) 173
BpiI GAAGAC 1 cut(s) 1126
BpmI CTGGAG 1 cut(s) 821
BpuMI CCSGG 4 cut(s) 461, 605, 985, 986
BsaHI GRCGYC 5 cut(s) 79, 138, 378, 752, 878
BsaJI CCNNGG 6 cut(s) 603, 716, 829, 857, 984, 1138
BsaWI WCCGGW 4 cut(s) 465, 579, 618, 930
BsaXI ACNNNNNCTCC 4 cut(s) 185, 206, 215, 236
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 591, 625, 870
Bse118I RCCGGY 2 cut(s) 579, 618
Bse1I ACTGG 3 cut(s) 125, 274, 484
Bse3DI GCAATG 2 cut(s) 391, 534
BseBI CCWGG 3 cut(s) 232, 824, 839
BseDI CCNNGG 6 cut(s) 603, 716, 829, 857, 984, 1138
BseGI GGATG 1 cut(s) 1173
BseLI CCNNNNNNNGG 4 cut(s) 231, 591, 625, 870
BseMI GCAATG 2 cut(s) 391, 534
BseMII CTCAG 1 cut(s) 88
BseNI ACTGG 3 cut(s) 125, 274, 484
BsePI GCGCGC 1 cut(s) 711
BseXI GCAGC 5 cut(s) 44, 372, 694, 879, 1087
BseYI CCCAGC 1 cut(s) 35
BsgI GTGCAG 1 cut(s) 878
Bsh1236I CGCG 6 cut(s) 8, 240, 396, 661, 713, 831
Bsh1285I CGRYCG 4 cut(s) 334, 460, 623, 665
BshFI GGCC 8 cut(s) 322, 483, 506, 591, 608, 630, 721, 834
BshNI GGYRCC 2 cut(s) 137, 877
BshTI ACCGGT 2 cut(s) 579, 618
BsiEI CGRYCG 4 cut(s) 334, 460, 623, 665
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 1 cut(s) 984
BsiSI CCGG 9 cut(s) 460, 466, 490, 580, 592, 605, 619, 931, 985
BslFI GGGAC 2 cut(s) 476, 941
BslI CCNNNNNNNGG 4 cut(s) 231, 591, 625, 870
BsmAI GTCTC 1 cut(s) 661
BsmBI CGTCTC 1 cut(s) 661
BsmFI GGGAC 2 cut(s) 476, 941
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 8 cut(s) 322, 483, 506, 591, 608, 630, 721, 834
BsoBI CYCGRG 1 cut(s) 984
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 2 cut(s) 638, 969
Bsp19I CCATGG 1 cut(s) 1138
Bsp68I TCGCGA 1 cut(s) 396
BspANI GGCC 8 cut(s) 322, 483, 506, 591, 608, 630, 721, 834
BspCNI CTCAG 1 cut(s) 87
BspFNI CGCG 6 cut(s) 8, 240, 396, 661, 713, 831
BspLI GGNNCC 6 cut(s) 139, 236, 464, 879, 929, 1107
BspT107I GGYRCC 2 cut(s) 137, 877
BsrDI GCAATG 2 cut(s) 391, 534
BsrFI RCCGGY 2 cut(s) 579, 618
BsrI ACTGG 3 cut(s) 125, 274, 484
BssAI RCCGGY 2 cut(s) 579, 618
BssECI CCNNGG 6 cut(s) 603, 716, 829, 857, 984, 1138
BssHII GCGCGC 1 cut(s) 711
BssMI GATC 2 cut(s) 638, 969
BssNI GRCGYC 5 cut(s) 79, 138, 378, 752, 878
BssSI CACGAG 1 cut(s) 896
BssT1I CCWWGG 1 cut(s) 1138
Bst2BI CACGAG 1 cut(s) 896
Bst2UI CCWGG 3 cut(s) 232, 824, 839
Bst4CI ACNGT 1 cut(s) 786
BstACI GRCGYC 5 cut(s) 79, 138, 378, 752, 878
BstC8I GCNNGC 6 cut(s) 6, 320, 324, 495, 713, 750
BstDEI CTNAG 1 cut(s) 74
BstDSI CCRYGG 2 cut(s) 829, 1138
BstF5I GGATG 1 cut(s) 1173
BstFNI CGCG 6 cut(s) 8, 240, 396, 661, 713, 831
BstH2I RGCGCY 5 cut(s) 58, 141, 374, 803, 881
BstKTI GATC 2 cut(s) 641, 972
BstMAI GTCTC 1 cut(s) 661
BstMBI GATC 2 cut(s) 638, 969
BstMCI CGRYCG 4 cut(s) 334, 460, 623, 665
BstMWI GCNNNNNNNGC 8 cut(s) 78, 261, 415, 503, 806, 828, 889, 1075
BstNI CCWGG 3 cut(s) 232, 824, 839
BstPAI GACNNNNGTC 1 cut(s) 173
BstSCI CCNGG 7 cut(s) 230, 459, 603, 822, 837, 983, 984
BstSFI CTRYAG 1 cut(s) 686
BstUI CGCG 6 cut(s) 8, 240, 396, 661, 713, 831
BstV1I GCAGC 5 cut(s) 44, 372, 694, 879, 1087
BstV2I GAAGAC 1 cut(s) 1126
BstXI CCANNNNNNTGG 1 cut(s) 748
BsuRI GGCC 8 cut(s) 322, 483, 506, 591, 608, 630, 721, 834
BtgI CCRYGG 2 cut(s) 829, 1138
BtrI CACGTC 1 cut(s) 175
BtsCI GGATG 1 cut(s) 1173
BtsI GCAGTG 1 cut(s) 425
BtsIMutI CAGTG 1 cut(s) 425
BtuMI TCGCGA 1 cut(s) 396
Cac8I GCNNGC 6 cut(s) 6, 320, 324, 495, 713, 750
CaiI CAGNNNCTG 1 cut(s) 430
Cfr10I RCCGGY 2 cut(s) 579, 618
Cfr42I CCGCGG 1 cut(s) 832
Cfr9I CCCGGG 1 cut(s) 984
CseI GACGC 4 cut(s) 87, 352, 643, 741
CspAI ACCGGT 2 cut(s) 579, 618
CviAII CATG 3 cut(s) 130, 733, 1139
DdeI CTNAG 1 cut(s) 74
DinI GGCGCC 2 cut(s) 139, 879
DpnI GATC 2 cut(s) 640, 971
DpnII GATC 2 cut(s) 638, 969
DriI GACNNNNNGTC 1 cut(s) 619
EaeI YGGCCR 2 cut(s) 589, 606
Eam1105I GACNNNNNGTC 1 cut(s) 619
Eco130I CCWWGG 1 cut(s) 1138
Eco47I GGWCC 7 cut(s) 463, 582, 826, 841, 861, 928, 1105
Eco88I CYCGRG 1 cut(s) 984
EcoO109I RGGNCCY 2 cut(s) 505, 861
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 3 cut(s) 230, 822, 837
EcoT14I CCWWGG 1 cut(s) 1138
EgeI GGCGCC 2 cut(s) 139, 879
EheI GGCGCC 2 cut(s) 139, 879
ErhI CCWWGG 1 cut(s) 1138
Esp3I CGTCTC 1 cut(s) 661
FaeI CATG 3 cut(s) 133, 736, 1142
FaiI YATR 4 cut(s) 131, 734, 992, 1140
FaqI GGGAC 2 cut(s) 476, 941
FatI CATG 3 cut(s) 129, 732, 1138
FauI CCCGC 3 cut(s) 13, 331, 918
FblI GTMKAC 1 cut(s) 330
FokI GGATG 1 cut(s) 1180
FspBI CTAG 1 cut(s) 533
GsaI CCCAGC 1 cut(s) 39
GsuI CTGGAG 1 cut(s) 821
HaeII RGCGCY 5 cut(s) 58, 141, 374, 803, 881
HaeIII GGCC 8 cut(s) 322, 483, 506, 591, 608, 630, 721, 834
HapII CCGG 9 cut(s) 460, 466, 490, 580, 592, 605, 619, 931, 985
HgaI GACGC 4 cut(s) 87, 352, 643, 741
Hin1I GRCGYC 5 cut(s) 79, 138, 378, 752, 878
Hin1II CATG 3 cut(s) 133, 736, 1142
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HinfI GANTC 8 cut(s) 391, 469, 814, 853, 914, 961, 1007, 1172
HpaII CCGG 9 cut(s) 460, 466, 490, 580, 592, 605, 619, 931, 985
HphI GGTGA 2 cut(s) 260, 757
Hpy166II GTNNAC 3 cut(s) 331, 765, 844
Hpy188I TCNGA 5 cut(s) 77, 346, 638, 858, 969
Hpy188III TCNNGA 8 cut(s) 149, 166, 395, 455, 850, 973, 1064, 1112
Hpy8I GTNNAC 3 cut(s) 331, 765, 844
Hpy99I CGWCG 8 cut(s) 13, 31, 179, 254, 332, 380, 401, 757
HpyAV CCTTC 6 cut(s) 172, 473, 496, 874, 949, 1034
HpyCH4III ACNGT 1 cut(s) 786
HpyCH4IV ACGT 3 cut(s) 174, 312, 378
HpyCH4V TGCA 3 cut(s) 384, 527, 895
HpyF10VI GCNNNNNNNGC 8 cut(s) 78, 261, 415, 503, 806, 828, 889, 1075
HpyF3I CTNAG 1 cut(s) 74
HpySE526I ACGT 3 cut(s) 174, 312, 378
Hsp92I GRCGYC 5 cut(s) 79, 138, 378, 752, 878
Hsp92II CATG 3 cut(s) 133, 736, 1142
KasI GGCGCC 2 cut(s) 137, 877
KspI CCGCGG 1 cut(s) 832
Kzo9I GATC 2 cut(s) 638, 969
LmnI GCTCC 8 cut(s) 62, 240, 288, 554, 594, 651, 1066, 1152
Lsp1109I GCAGC 5 cut(s) 44, 372, 694, 879, 1087
MaeI CTAG 1 cut(s) 533
MaeII ACGT 3 cut(s) 174, 312, 378
MalI GATC 2 cut(s) 640, 971
MboI GATC 2 cut(s) 638, 969
MboII GAAGA 1 cut(s) 1126
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 5 cut(s) 14, 47, 772, 998, 1143
Mly113I GGCGCC 2 cut(s) 138, 878
MlyI GAGTC 3 cut(s) 463, 923, 1166
MmeI TCCRAC 5 cut(s) 214, 333, 414, 576, 1083
MspA1I CMGCKG 3 cut(s) 35, 831, 883
MspI CCGG 9 cut(s) 460, 466, 490, 580, 592, 605, 619, 931, 985
MspR9I CCNGG 7 cut(s) 232, 461, 605, 824, 839, 985, 986
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 3 cut(s) 232, 824, 839
MvnI CGCG 6 cut(s) 8, 240, 396, 661, 713, 831
MwoI GCNNNNNNNGC 8 cut(s) 78, 261, 415, 503, 806, 828, 889, 1075
NarI GGCGCC 2 cut(s) 138, 878
NciI CCSGG 4 cut(s) 461, 605, 985, 986
NcoI CCATGG 1 cut(s) 1138
NdeII GATC 2 cut(s) 638, 969
NlaIII CATG 3 cut(s) 133, 736, 1142
NlaIV GGNNCC 6 cut(s) 139, 236, 464, 879, 929, 1107
NmeAIII GCCGAG 2 cut(s) 453, 741
NruI TCGCGA 1 cut(s) 396
PauI GCGCGC 1 cut(s) 711
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 1 cut(s) 264
PfeI GAWTC 5 cut(s) 391, 814, 853, 961, 1007
PinAI ACCGGT 2 cut(s) 579, 618
PleI GAGTC 3 cut(s) 463, 922, 1166
PluTI GGCGCC 2 cut(s) 141, 881
PpsI GAGTC 3 cut(s) 463, 922, 1166
PpuMI RGGWCCY 1 cut(s) 861
PshAI GACNNNNGTC 1 cut(s) 173
Psp5II RGGWCCY 1 cut(s) 861
Psp6I CCWGG 3 cut(s) 230, 822, 837
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 3 cut(s) 230, 822, 837
PspN4I GGNNCC 6 cut(s) 139, 236, 464, 879, 929, 1107
PspPPI RGGWCCY 1 cut(s) 861
PstNI CAGNNNCTG 1 cut(s) 430
PteI GCGCGC 1 cut(s) 711
PvuII CAGCTG 1 cut(s) 35
RruI TCGCGA 1 cut(s) 396
SacII CCGCGG 1 cut(s) 832
SalI GTCGAC 1 cut(s) 329
Sau3AI GATC 2 cut(s) 638, 969
SchI GAGTC 3 cut(s) 463, 923, 1166
ScrFI CCNGG 7 cut(s) 232, 461, 605, 824, 839, 985, 986
SduI GDGCHC 1 cut(s) 447
SfcI CTRYAG 1 cut(s) 686
SfoI GGCGCC 2 cut(s) 139, 879
Sfr303I CCGCGG 1 cut(s) 832
SgrBI CCGCGG 1 cut(s) 832
SinI GGWCC 7 cut(s) 463, 582, 826, 841, 861, 928, 1105
SmaI CCCGGG 1 cut(s) 986
Sse9I AATT 5 cut(s) 14, 47, 772, 998, 1143
SspDI GGCGCC 2 cut(s) 137, 877
SspMI CTAG 1 cut(s) 533
StyD4I CCNGG 7 cut(s) 230, 459, 603, 822, 837, 983, 984
StyI CCWWGG 1 cut(s) 1138
TaaI ACNGT 1 cut(s) 786
TaiI ACGT 3 cut(s) 177, 315, 381
TasI AATT 5 cut(s) 14, 47, 772, 998, 1143
TauI GCSGC 7 cut(s) 84, 677, 812, 834, 878, 883, 925
TfiI GAWTC 5 cut(s) 391, 814, 853, 961, 1007
TscAI CASTG 1 cut(s) 425
TseI GCWGC 5 cut(s) 32, 360, 682, 892, 1075
TspDTI ATGAA 2 cut(s) 741, 953
TspGWI ACGGA 4 cut(s) 26, 52, 807, 1011
TspMI CCCGGG 1 cut(s) 984
TspRI CASTG 1 cut(s) 425
VpaK11BI GGWCC 7 cut(s) 463, 582, 826, 841, 861, 928, 1105
XapI RAATTY 1 cut(s) 14
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XmaI CCCGGG 1 cut(s) 984
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 1 cut(s) 533
ZraI GACGTC 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.