MD10G1025900.v1.1

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010852
Physical Location & Seq
Forward (+)
3228640 .. 3229812
1173 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1025900.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGCCAGCGACGGAATACTCGTCAGCTGGTTCCAACCAAATCGGACGGTCAATCTTCGGCCTCAGACGCGATCAGTTGAATTCAATGGAAACCCACGGCCTTTCAGGTGGTGGTGGCGGTGGTAGTGGTGGATTGAACCTCGATTCCTTCCAAACCCACGTCGCCGATTGCTTCCTCCAACTCTCCTCCTCCCACGACCTCCTCTCCCTCCCATGGCTCCGAGACCTCCTCGACTCCTTCCTCCGCATACACCAGGAGTTCAAAACCCTCCTCATCCTCTCCACCAAAACCCACATCTCCAAACCCACCGTTGACCGCTACATCTCCGACTACTTCGAGCGCAGCGTCAAAGCCCTAGACGTCTGCAACGCCATCCGCGACGGAATCGAGGAGATGCGCCAGTGGCTCAAGCTGCTCGACATCGTCCTCGTCGCGCTTGACCGCGACAGGACACTCGGCGAGGGCCAATTTCGCCGCGCGAAGAAGGCTCTCGTCGACCTGGCCATCGCAATGCTCGACGACAAGGACTCCTCCAACACCACCATAGCCCACCGGAACCGCTCCTTCGGCCGAAACAACAACACAAAGGATCATCACCAGCAGCAGCACCGGTCGCTCGGCCACTTCCGATCTCTGTCCTGGAGCGTCTCGCGGTCGTGGTCGGCCGCGAGGCAGCTGCAGGCAATCGGGAACAATCTATACGCCCCTAGGCCCAATGAAATTGTGGCCTCCAGCGGGCTCGCGCCGGCGGTTTTCACAATCAATTCGGTTTTGCTGTTCGTAATGTGGGCCCTGGTGGCGGCGATCCCCTGCCAGGACAGGGGACTGCAGGTGCATTTCAACGTGCCGAGAAGCTTCGTGTGGGCGGGGTCAATGCTGTCTCTGCACGATCGGATTATGGACGAGTCGAAGCGGCGGGACCGGAGAAATGCCTGCGCATTGTTGAAGGAGATTCATCAGATCGAAAGGTGCTCGCGGGTGATCGGCGATCTGGCAGACTCGGCGCACTTCCCGCTTGCGGAGGCGAAGGAGAAGGAGGTGAGGGAGAGAGTGCAGCAACTGTCGAGTCTCTGCGAGGGGATAAAGGAGGGGTTGGATCCTCTGGAGCGGCAGGTGAGGGAGGTGTTCCATGCCATTGTAAAAAGCCGAACGGAGGGGATGGACTCGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

391

Amino Acids

43.88

Weight (kDa)

8.71

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 1 - 389 5.9e-149 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18740 AT1G74450
fragaria_vesca FvH4_2g13680 FvH4_2g13680
malus_domestica MD05G1026700.v1.1 MD10G1025900.v1.1
prunus_persica Prupe.8G031200_v2.0.a1
pyrus_communis pycom05g01850 pycom10g01740
rosa_chinensis RchiOBHm_Chr6g0275361
rosa_laevigata RLG00000013471
rosa_multiflora Rmu_co8417379.1_g000001 Rmu_sc0004143.1_g000001 Rmu_sc0019329.1_g000002
rosa_roxburghii Rroxscaffold_7G00193380
rosa_rugosa Rorug06G0091100
rosa_samantha Rh6AG205000 Rh6CG210200 Rh6DG200500
rosa_wichuraiana Rw6G017790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 820, 1102
AatII GACGTC 1 cut(s) 363
Acc16I TGCGCA 1 cut(s) 937
Acc36I ACCTGC 2 cut(s) 820, 1102
AccBSI CCGCTC 2 cut(s) 561, 1108
AccI GTMKAC 1 cut(s) 495
AclWI GGATC 4 cut(s) 597, 799, 1091, 1104
AcoI YGGCCR 4 cut(s) 501, 568, 619, 663
AcsI RAATTY 1 cut(s) 79
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 1 cut(s) 1169
AfiI CCNNNNNNNGG 7 cut(s) 213, 735, 799, 814, 820, 1018, 1153
AgeI ACCGGT 1 cut(s) 609
AgsI TTSAA 6 cut(s) 79, 84, 136, 262, 841, 946
AjiI CACGTC 1 cut(s) 160
AjnI CCWGG 5 cut(s) 252, 498, 638, 792, 813
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 4 cut(s) 26, 412, 676, 855
AluI AGCT 4 cut(s) 26, 412, 676, 855
Alw21I GWGCWC 1 cut(s) 974
Alw26I GTCTC 4 cut(s) 216, 652, 885, 1073
AlwI GGATC 4 cut(s) 597, 799, 1091, 1104
AlwNI CAGNNNCTG 1 cut(s) 1060
ApaI GGGCCC 1 cut(s) 793
ApeKI GCWGC 7 cut(s) 342, 412, 601, 604, 673, 676, 1054
ApoI RAATTY 1 cut(s) 79
AsiGI ACCGGT 1 cut(s) 609
AspA2I CCTAGG 1 cut(s) 707
AspLEI GCGC 7 cut(s) 342, 399, 436, 479, 745, 938, 1006
AspS9I GGNCC 5 cut(s) 463, 711, 789, 790, 919
AsuHPI GGTGA 4 cut(s) 587, 991, 1051, 1126
AvaII GGWCC 1 cut(s) 919
AvrII CCTAGG 1 cut(s) 707
BaeGI GKGCMC 1 cut(s) 793
BalI TGGCCA 1 cut(s) 503
BamHI GGATCC 1 cut(s) 1096
BanII GRGCYC 2 cut(s) 741, 793
Bbv12I GWGCWC 1 cut(s) 974
BbvI GCAGC 7 cut(s) 354, 399, 613, 616, 663, 685, 1066
BccI CCATC 3 cut(s) 380, 512, 1153
BceAI ACGGC 1 cut(s) 112
BcgI CGANNNNNNTGC 2 cut(s) 658, 692
BciT130I CCWGG 5 cut(s) 254, 500, 640, 794, 815
BcoDI GTCTC 4 cut(s) 216, 652, 885, 1073
BfaI CTAG 2 cut(s) 356, 708
BfmI CTRYAG 2 cut(s) 677, 827
BfuAI ACCTGC 2 cut(s) 820, 1102
BglI GCCNNNNNGGC 1 cut(s) 797
BlnI CCTAGG 1 cut(s) 707
Bme1390I CCNGG 5 cut(s) 254, 500, 640, 794, 815
Bme18I GGWCC 1 cut(s) 919
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 5 cut(s) 463, 711, 789, 790, 919
BmiI GGNNCC 6 cut(s) 31, 218, 557, 791, 920, 1098
BmrFI CCNGG 5 cut(s) 254, 500, 640, 794, 815
BmsI GCATC 1 cut(s) 384
BplI GAGNNNNNCTC 2 cut(s) 213, 245
BpmI CTGGAG 3 cut(s) 661, 715, 1124
BpuEI CTTGAG 1 cut(s) 392
BsaHI GRCGYC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 216
BsaJI CCNNGG 4 cut(s) 94, 212, 707, 792
BsaWI WCCGGW 3 cut(s) 552, 609, 921
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 512, 542
Bsc4I CCNNNNNNNGG 7 cut(s) 213, 735, 799, 814, 820, 1018, 1153
Bse118I RCCGGY 2 cut(s) 609, 745
Bse1I ACTGG 1 cut(s) 400
Bse3DI GCAATG 1 cut(s) 516
BseBI CCWGG 5 cut(s) 254, 500, 640, 794, 815
BseDI CCNNGG 4 cut(s) 94, 212, 707, 792
BseGI GGATG 3 cut(s) 273, 372, 1164
BseLI CCNNNNNNNGG 7 cut(s) 213, 735, 799, 814, 820, 1018, 1153
BseMI GCAATG 1 cut(s) 516
BseMII CTCAG 1 cut(s) 76
BseNI ACTGG 1 cut(s) 400
BseRI GAGGAG 7 cut(s) 175, 178, 191, 218, 260, 404, 520
BseSI GKGCMC 1 cut(s) 793
BseX3I CGGCCG 2 cut(s) 568, 663
BseXI GCAGC 7 cut(s) 354, 399, 613, 616, 663, 685, 1066
BsgI GTGCAG 2 cut(s) 869, 1073
Bsh1285I CGRYCG 5 cut(s) 571, 614, 656, 666, 892
BshTI ACCGGT 1 cut(s) 609
BsiEI CGRYCG 5 cut(s) 571, 614, 656, 666, 892
BsiHKAI GWGCWC 1 cut(s) 974
BsiSI CCGG 4 cut(s) 553, 610, 746, 922
BslFI GGGAC 2 cut(s) 837, 932
BslI CCNNNNNNNGG 7 cut(s) 213, 735, 799, 814, 820, 1018, 1153
BsmAI GTCTC 4 cut(s) 216, 652, 885, 1073
BsmBI CGTCTC 1 cut(s) 652
BsmFI GGGAC 2 cut(s) 837, 932
Bso31I GGTCTC 1 cut(s) 216
Bsp120I GGGCCC 1 cut(s) 789
Bsp1286I GDGCHC 3 cut(s) 741, 793, 974
Bsp143I GATC 9 cut(s) 70, 589, 629, 804, 889, 960, 981, 988, 1096
Bsp19I CCATGG 1 cut(s) 212
BspCNI CTCAG 1 cut(s) 75
BspLI GGNNCC 6 cut(s) 31, 218, 557, 791, 920, 1098
BspMAI CTGCAG 2 cut(s) 681, 831
BspMI ACCTGC 2 cut(s) 820, 1102
BspPI GGATC 4 cut(s) 597, 799, 1091, 1104
BspTNI GGTCTC 1 cut(s) 216
BsrBI CCGCTC 2 cut(s) 561, 1108
BsrDI GCAATG 1 cut(s) 516
BsrFI RCCGGY 2 cut(s) 609, 745
BsrI ACTGG 1 cut(s) 400
BssAI RCCGGY 2 cut(s) 609, 745
BssECI CCNNGG 4 cut(s) 94, 212, 707, 792
BssMI GATC 9 cut(s) 70, 589, 629, 804, 889, 960, 981, 988, 1096
BssNI GRCGYC 1 cut(s) 360
BssT1I CCWWGG 2 cut(s) 212, 707
Bst2UI CCWGG 5 cut(s) 254, 500, 640, 794, 815
Bst4CI ACNGT 3 cut(s) 48, 310, 1062
BstACI GRCGYC 1 cut(s) 360
BstC8I GCNNGC 8 cut(s) 6, 681, 737, 741, 747, 934, 974, 1017
BstDEI CTNAG 1 cut(s) 62
BstDSI CCRYGG 2 cut(s) 94, 212
BstF5I GGATG 3 cut(s) 273, 372, 1164
BstHHI GCGC 7 cut(s) 342, 399, 436, 479, 745, 938, 1006
BstKTI GATC 9 cut(s) 73, 592, 632, 807, 892, 963, 984, 991, 1099
BstMAI GTCTC 4 cut(s) 216, 652, 885, 1073
BstMBI GATC 9 cut(s) 70, 589, 629, 804, 889, 960, 981, 988, 1096
BstMCI CGRYCG 5 cut(s) 571, 614, 656, 666, 892
BstNI CCWGG 5 cut(s) 254, 500, 640, 794, 815
BstSCI CCNGG 5 cut(s) 252, 498, 638, 792, 813
BstSFI CTRYAG 2 cut(s) 677, 827
BstSLI GKGCMC 1 cut(s) 793
BstV1I GCAGC 7 cut(s) 354, 399, 613, 616, 663, 685, 1066
BstX2I RGATCY 1 cut(s) 1096
BstYI RGATCY 1 cut(s) 1096
BstZI CGGCCG 2 cut(s) 568, 663
BtgI CCRYGG 2 cut(s) 94, 212
BtgZI GCGATG 1 cut(s) 490
BtrI CACGTC 1 cut(s) 160
BtsCI GGATG 3 cut(s) 273, 372, 1164
BtsIMutI CAGTG 1 cut(s) 407
BveI ACCTGC 2 cut(s) 820, 1102
Cac8I GCNNGC 8 cut(s) 6, 681, 737, 741, 747, 934, 974, 1017
CaiI CAGNNNCTG 1 cut(s) 1060
CfoI GCGC 7 cut(s) 342, 399, 436, 479, 745, 938, 1006
Cfr10I RCCGGY 2 cut(s) 609, 745
Cfr13I GGNCC 5 cut(s) 463, 711, 789, 790, 919
CseI GACGC 3 cut(s) 75, 334, 634
Csp6I GTAC 1 cut(s) 1168
CspAI ACCGGT 1 cut(s) 609
CviAII CATG 2 cut(s) 213, 1130
CviQI GTAC 1 cut(s) 1168
DdeI CTNAG 1 cut(s) 62
DpnI GATC 9 cut(s) 72, 591, 631, 806, 891, 962, 983, 990, 1098
DpnII GATC 9 cut(s) 70, 589, 629, 804, 889, 960, 981, 988, 1096
EaeI YGGCCR 4 cut(s) 501, 568, 619, 663
EagI CGGCCG 2 cut(s) 568, 663
EclXI CGGCCG 2 cut(s) 568, 663
Eco130I CCWWGG 2 cut(s) 212, 707
Eco24I GRGCYC 2 cut(s) 741, 793
Eco31I GGTCTC 1 cut(s) 216
Eco47I GGWCC 1 cut(s) 919
Eco52I CGGCCG 2 cut(s) 568, 663
EcoO109I RGGNCCY 1 cut(s) 790
EcoRI GAATTC 1 cut(s) 79
EcoRII CCWGG 5 cut(s) 252, 498, 638, 792, 813
EcoT14I CCWWGG 2 cut(s) 212, 707
EcoT38I GRGCYC 2 cut(s) 741, 793
ErhI CCWWGG 2 cut(s) 212, 707
Esp3I CGTCTC 1 cut(s) 652
FaeI CATG 2 cut(s) 216, 1133
FaiI YATR 6 cut(s) 214, 248, 545, 700, 899, 1131
FaqI GGGAC 2 cut(s) 837, 932
FatI CATG 2 cut(s) 212, 1129
FauI CCCGC 5 cut(s) 728, 859, 909, 969, 1020
FblI GTMKAC 1 cut(s) 495
FokI GGATG 2 cut(s) 260, 359
FriOI GRGCYC 2 cut(s) 741, 793
FspBI CTAG 2 cut(s) 356, 708
FspI TGCGCA 1 cut(s) 937
GlaI GCGC 7 cut(s) 341, 398, 435, 478, 744, 937, 1005
GsuI CTGGAG 3 cut(s) 661, 715, 1124
HapII CCGG 4 cut(s) 553, 610, 746, 922
HgaI GACGC 3 cut(s) 75, 334, 634
HhaI GCGC 7 cut(s) 342, 399, 436, 479, 745, 938, 1006
Hin1I GRCGYC 1 cut(s) 360
Hin1II CATG 2 cut(s) 216, 1133
Hin6I GCGC 7 cut(s) 340, 397, 434, 477, 743, 936, 1004
HinP1I GCGC 7 cut(s) 340, 397, 434, 477, 743, 936, 1004
HincII GTYRAC 2 cut(s) 313, 496
HindII GTYRAC 2 cut(s) 313, 496
HindIII AAGCTT 1 cut(s) 853
HinfI GANTC 9 cut(s) 143, 233, 384, 527, 905, 952, 998, 1066, 1163
HpaII CCGG 4 cut(s) 553, 610, 746, 922
HphI GGTGA 4 cut(s) 587, 991, 1051, 1126
Hpy166II GTNNAC 2 cut(s) 313, 496
Hpy188I TCNGA 7 cut(s) 44, 65, 221, 328, 629, 894, 960
Hpy188III TCNNGA 2 cut(s) 688, 1103
Hpy8I GTNNAC 2 cut(s) 313, 496
Hpy99I CGWCG 6 cut(s) 13, 164, 383, 434, 497, 521
HpyAV CCTTC 7 cut(s) 157, 247, 478, 574, 940, 1021, 1027
HpyCH4III ACNGT 3 cut(s) 48, 310, 1062
HpyCH4IV ACGT 3 cut(s) 159, 360, 843
HpyCH4V TGCA 6 cut(s) 366, 679, 829, 835, 886, 1054
HpyF3I CTNAG 1 cut(s) 62
HpySE526I ACGT 3 cut(s) 159, 360, 843
Hsp92I GRCGYC 1 cut(s) 360
Hsp92II CATG 2 cut(s) 216, 1133
HspAI GCGC 7 cut(s) 340, 397, 434, 477, 743, 936, 1004
KroI GCCGGC 1 cut(s) 745
KroNI GCCGGC 1 cut(s) 747
Kzo9I GATC 9 cut(s) 70, 589, 629, 804, 889, 960, 981, 988, 1096
LmnI GCTCC 4 cut(s) 222, 566, 642, 1105
Lsp1109I GCAGC 7 cut(s) 354, 399, 613, 616, 663, 685, 1066
LweI GCATC 1 cut(s) 384
MaeI CTAG 2 cut(s) 356, 708
MaeII ACGT 3 cut(s) 159, 360, 843
MalI GATC 9 cut(s) 72, 591, 631, 806, 891, 962, 983, 990, 1098
MbiI CCGCTC 2 cut(s) 561, 1108
MboI GATC 9 cut(s) 70, 589, 629, 804, 889, 960, 981, 988, 1096
MboII GAAGA 2 cut(s) 46, 493
MflI RGATCY 1 cut(s) 1096
MhlI GDGCHC 3 cut(s) 741, 793, 974
MlsI TGGCCA 1 cut(s) 503
MluCI AATT 4 cut(s) 79, 467, 720, 763
MluNI TGGCCA 1 cut(s) 503
MlyI GAGTC 6 cut(s) 227, 521, 914, 992, 1075, 1157
MmeI TCCRAC 5 cut(s) 57, 202, 351, 558, 1074
Mox20I TGGCCA 1 cut(s) 503
MreI CGCCGGCG 1 cut(s) 745
MroNI GCCGGC 1 cut(s) 745
MscI TGGCCA 1 cut(s) 503
MslI CAYNNNNRTG 1 cut(s) 509
Msp20I TGGCCA 1 cut(s) 503
MspA1I CMGCKG 3 cut(s) 26, 676, 735
MspI CCGG 4 cut(s) 553, 610, 746, 922
MspR9I CCNGG 5 cut(s) 254, 500, 640, 794, 815
MvaI CCWGG 5 cut(s) 254, 500, 640, 794, 815
NaeI GCCGGC 1 cut(s) 747
NcoI CCATGG 1 cut(s) 212
NdeII GATC 9 cut(s) 70, 589, 629, 804, 889, 960, 981, 988, 1096
NgoMIV GCCGGC 1 cut(s) 745
NlaIII CATG 2 cut(s) 216, 1133
NlaIV GGNNCC 6 cut(s) 31, 218, 557, 791, 920, 1098
NmeAIII GCCGAG 4 cut(s) 435, 597, 873, 980
NsbI TGCGCA 1 cut(s) 937
PaqCI CACCTGC 2 cut(s) 820, 1102
PcsI WCGNNNNNNNCGW 4 cut(s) 17, 342, 375, 513
PdiI GCCGGC 1 cut(s) 747
PfeI GAWTC 3 cut(s) 143, 384, 952
PflFI GACNNNGTC 1 cut(s) 422
PfoI TCCNGGA 1 cut(s) 638
PinAI ACCGGT 1 cut(s) 609
Ple19I CGATCG 1 cut(s) 892
PleI GAGTC 6 cut(s) 227, 521, 913, 992, 1074, 1157
PpsI GAGTC 6 cut(s) 227, 521, 913, 992, 1074, 1157
Psp6I CCWGG 5 cut(s) 252, 498, 638, 792, 813
PspGI CCWGG 5 cut(s) 252, 498, 638, 792, 813
PspN4I GGNNCC 6 cut(s) 31, 218, 557, 791, 920, 1098
PspOMI GGGCCC 1 cut(s) 789
PspPI GGNCC 5 cut(s) 463, 711, 789, 790, 919
PsrI GAACNNNNNNTAC 2 cut(s) 683, 715
PstI CTGCAG 2 cut(s) 681, 831
PstNI CAGNNNCTG 1 cut(s) 1060
PsuI RGATCY 1 cut(s) 1096
PsyI GACNNNGTC 1 cut(s) 422
PvuI CGATCG 1 cut(s) 892
PvuII CAGCTG 2 cut(s) 26, 676
RsaI GTAC 1 cut(s) 1169
RsaNI GTAC 1 cut(s) 1168
RseI CAYNNNNRTG 1 cut(s) 509
SalI GTCGAC 1 cut(s) 494
Sau3AI GATC 9 cut(s) 70, 589, 629, 804, 889, 960, 981, 988, 1096
Sau96I GGNCC 5 cut(s) 463, 711, 789, 790, 919
SchI GAGTC 6 cut(s) 227, 521, 914, 992, 1075, 1157
ScrFI CCNGG 5 cut(s) 254, 500, 640, 794, 815
SduI GDGCHC 3 cut(s) 741, 793, 974
SfaNI GCATC 1 cut(s) 384
SfcI CTRYAG 2 cut(s) 677, 827
SgrAI CRCCGGYG 1 cut(s) 745
SinI GGWCC 1 cut(s) 919
SmiMI CAYNNNNRTG 1 cut(s) 509
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 4 cut(s) 79, 467, 720, 763
SspMI CTAG 2 cut(s) 356, 708
StyD4I CCNGG 5 cut(s) 252, 498, 638, 792, 813
StyI CCWWGG 2 cut(s) 212, 707
TaaI ACNGT 3 cut(s) 48, 310, 1062
TaiI ACGT 3 cut(s) 162, 363, 846
TasI AATT 4 cut(s) 79, 467, 720, 763
TauI GCSGC 5 cut(s) 477, 668, 803, 916, 1111
TfiI GAWTC 3 cut(s) 143, 384, 952
TscAI CASTG 1 cut(s) 407
TseI GCWGC 7 cut(s) 342, 412, 601, 604, 673, 676, 1054
TspDTI ATGAA 2 cut(s) 732, 944
TspGWI ACGGA 3 cut(s) 26, 396, 1166
TspRI CASTG 1 cut(s) 407
Tth111I GACNNNGTC 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 919
XapI RAATTY 1 cut(s) 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 721
XmaJI CCTAGG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 495
XspI CTAG 2 cut(s) 356, 708
ZraI GACGTC 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.