Rh6DG200500

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010852
Physical Location & Seq
Reverse (-)
35777257 .. 35781376
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG200500.1

Sequence Viewer

Length: 1194 bp
ATGCCCGCGACGGAATTCCAAAGGTCGTCGGCAGCTGGGTTCAACCAATTCGGGCGCTCCTCCGTCTTCAGCCTCAGACGCCGCCCCCACCACCACCACCACCACAACCTCGTCAATCCAGTCATGGACGGCGCCAAAGCTCTCGACCTCGACACCTTCCAGACCCACGTCGCCGATTGCTTCCAACACCTCTCCTCCCCCTCCCAAGACCTCCTCTCCCTCTCCTGGCTCCGCGACCTCCTCGACGGCTTCCTCCGCATTCACCACCAGTTCAAGCTCCTCCTCCTCTCCACCCCAAAATCCAACGTCAGCAGCAGGCCCGCCGTCGACCGCCTCCTCTCCGATTACTTCGAGCGCAGCGTCAAGGCCCTCGACGTCTGCAATGCGATTCGCGACGGGATTGAGCAGATGAGGCAGTGGCAGAAGCTCTTGGAGATTGTGCTCTGCGCTCTCGACCGGGACCGGACTCTCGGCGAAGGCCAGTTCCGGCGAGCCAAGAAGGCCCTGATAGATTTGGCTATTGCAATGCTAGACGACAAGGACAGCTCCAACAACAACACCCTCGCCCACCGCAACCGGTCCTTTGGCCGGAGCAACGCCCCGGCCAAAGACTACCGGTCAATGGGCCACTTCCGATCTCTGTCGTGGAGCGTCTCGCGGTCGTGGTCGGCGGCGAGGCAGCTGCAGGCGATTGGGAACAACCTCTGCGCGCCGAGGCCCAATGAAGTCGTGGCGACCAATGGGCTGGCGTCGGCTGTGTTCACCATCAATTCTGTTCTACTGTTCGTAATGTGGGCGCTGGTGGCGGCGATTCCGTGCCAGGACCGCGGCCTCCAGGTCCACTTCACGATTCCGAGGACCTTCGGGTGGGCGGCGCCGCTGCTTTCGCTGCACGAGAGGATAATGGAGGAGTCGAAGCGGCGGGACCGGAGGAACGCCTGTGCGCTCTTGAAGGAGATTCATCAGATCGAGAAATGTTCCCGGGTCATAAGCGAATTGGCTGATTCGGTTCAGTTTCCGTTGAGTGAGGAGAAAGAAGGGGAGGTCAGGCAGAGAGTTCAGGAGCTTTCGCAGCTCTGCGAGGGGATAAAGGAGGGCTTGGACCCTCTGGAAAGACAGGTCAGGGAGGTCTTCCATGGAATTGTGCGGAGCAGAACAGAGGGGATGGACTCTTTGGGAAAGACCAATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

397

Amino Acids

45.02

Weight (kDa)

9.05

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 11 - 392 4.7e-147 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18740 AT1G74450
fragaria_vesca FvH4_2g13680 FvH4_2g13680
malus_domestica MD05G1026700.v1.1 MD10G1025900.v1.1
prunus_persica Prupe.8G031200_v2.0.a1
pyrus_communis pycom05g01850 pycom10g01740
rosa_chinensis RchiOBHm_Chr6g0275361
rosa_laevigata RLG00000013471
rosa_multiflora Rmu_co8417379.1_g000001 Rmu_sc0004143.1_g000001 Rmu_sc0019329.1_g000002
rosa_roxburghii Rroxscaffold_7G00193380
rosa_rugosa Rorug06G0091100
rosa_samantha Rh6AG205000 Rh6CG210200 Rh6DG200500
rosa_wichuraiana Rw6G017790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 378
AccB1I GGYRCC 2 cut(s) 131, 874
AccI GTMKAC 1 cut(s) 327
AccII CGCG 6 cut(s) 8, 234, 393, 658, 710, 828
AcoI YGGCCR 2 cut(s) 586, 603
AcsI RAATTY 1 cut(s) 14
AcuI CTGAAG 1 cut(s) 52
AcyI GRCGYC 5 cut(s) 79, 132, 375, 749, 875
AfiI CCNNNNNNNGG 4 cut(s) 225, 588, 622, 867
AgeI ACCGGT 2 cut(s) 576, 615
AgsI TTSAA 3 cut(s) 43, 274, 952
AhdI GACNNNNNGTC 1 cut(s) 616
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 3 cut(s) 224, 819, 834
AluBI AGCT 8 cut(s) 35, 140, 277, 427, 546, 682, 1066, 1075
AluI AGCT 8 cut(s) 35, 140, 277, 427, 546, 682, 1066, 1075
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 1 cut(s) 658
Ama87I CYCGRG 1 cut(s) 981
AoxI GGCC 9 cut(s) 317, 366, 478, 501, 586, 603, 625, 716, 829
ApeKI GCWGC 8 cut(s) 32, 312, 357, 679, 682, 880, 889, 1072
ApoI RAATTY 1 cut(s) 14
AsiGI ACCGGT 2 cut(s) 576, 615
AspLEI GCGC 9 cut(s) 57, 134, 357, 449, 710, 712, 799, 877, 946
AsuC2I CCSGG 4 cut(s) 458, 602, 982, 983
AsuHPI GGTGA 2 cut(s) 254, 754
AvaI CYCGRG 1 cut(s) 981
AvaII GGWCC 7 cut(s) 460, 579, 823, 838, 858, 925, 1102
BanI GGYRCC 2 cut(s) 131, 874
BauI CACGAG 1 cut(s) 893
BbsI GAAGAC 2 cut(s) 58, 1123
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 8 cut(s) 44, 324, 369, 669, 691, 867, 876, 1084
BccI CCATC 2 cut(s) 773, 1159
BceAI ACGGC 3 cut(s) 145, 262, 308
BcgI CGANNNNNNTGC 2 cut(s) 664, 698
BciT130I CCWGG 3 cut(s) 226, 821, 836
BcnI CCSGG 4 cut(s) 458, 602, 982, 983
BcoDI GTCTC 1 cut(s) 658
BfaI CTAG 1 cut(s) 530
BfmI CTRYAG 1 cut(s) 683
BfoI RGCGCY 4 cut(s) 58, 135, 800, 878
BglI GCCNNNNNGGC 1 cut(s) 500
Bme1390I CCNGG 7 cut(s) 226, 458, 602, 821, 836, 982, 983
Bme18I GGWCC 7 cut(s) 460, 579, 823, 838, 858, 925, 1102
BmeRI GACNNNNNGTC 1 cut(s) 616
BmeT110I CYCGRG 1 cut(s) 981
BmgBI CACGTC 1 cut(s) 169
BmiI GGNNCC 6 cut(s) 133, 230, 461, 876, 926, 1104
BmrFI CCNGG 7 cut(s) 226, 458, 602, 821, 836, 982, 983
BoxI GACNNNNGTC 1 cut(s) 167
BpiI GAAGAC 2 cut(s) 58, 1123
BpmI CTGGAG 1 cut(s) 818
BpuMI CCSGG 4 cut(s) 458, 602, 982, 983
BsaHI GRCGYC 5 cut(s) 79, 132, 375, 749, 875
BsaJI CCNNGG 6 cut(s) 600, 713, 826, 854, 981, 1135
BsaWI WCCGGW 4 cut(s) 462, 576, 615, 927
BsaXI ACNNNNNCTCC 4 cut(s) 179, 200, 209, 230
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 588, 622, 867
Bse118I RCCGGY 2 cut(s) 576, 615
Bse1I ACTGG 3 cut(s) 119, 268, 481
Bse3DI GCAATG 2 cut(s) 388, 531
BseBI CCWGG 3 cut(s) 226, 821, 836
BseDI CCNNGG 6 cut(s) 600, 713, 826, 854, 981, 1135
BseGI GGATG 1 cut(s) 1170
BseLI CCNNNNNNNGG 4 cut(s) 225, 588, 622, 867
BseMI GCAATG 2 cut(s) 388, 531
BseMII CTCAG 1 cut(s) 88
BseNI ACTGG 3 cut(s) 119, 268, 481
BsePI GCGCGC 1 cut(s) 708
BseXI GCAGC 8 cut(s) 44, 324, 369, 669, 691, 867, 876, 1084
BseYI CCCAGC 1 cut(s) 35
BsgI GTGCAG 1 cut(s) 875
Bsh1236I CGCG 6 cut(s) 8, 234, 393, 658, 710, 828
Bsh1285I CGRYCG 3 cut(s) 331, 457, 662
BshFI GGCC 9 cut(s) 319, 368, 480, 503, 588, 605, 627, 718, 831
BshNI GGYRCC 2 cut(s) 131, 874
BshTI ACCGGT 2 cut(s) 576, 615
BsiEI CGRYCG 3 cut(s) 331, 457, 662
BsiHKAI GWGCWC 1 cut(s) 444
BsiHKCI CYCGRG 1 cut(s) 981
BsiSI CCGG 9 cut(s) 457, 463, 487, 577, 589, 602, 616, 928, 982
BslFI GGGAC 2 cut(s) 473, 938
BslI CCNNNNNNNGG 4 cut(s) 225, 588, 622, 867
BsmAI GTCTC 1 cut(s) 658
BsmBI CGTCTC 1 cut(s) 658
BsmFI GGGAC 2 cut(s) 473, 938
BsmI GAATGC 1 cut(s) 258
BsnI GGCC 9 cut(s) 319, 368, 480, 503, 588, 605, 627, 718, 831
BsoBI CYCGRG 1 cut(s) 981
Bsp1286I GDGCHC 1 cut(s) 444
Bsp143I GATC 2 cut(s) 635, 966
Bsp19I CCATGG 1 cut(s) 1135
Bsp68I TCGCGA 1 cut(s) 393
BspANI GGCC 9 cut(s) 319, 368, 480, 503, 588, 605, 627, 718, 831
BspCNI CTCAG 1 cut(s) 87
BspFNI CGCG 6 cut(s) 8, 234, 393, 658, 710, 828
BspLI GGNNCC 6 cut(s) 133, 230, 461, 876, 926, 1104
BspMAI CTGCAG 1 cut(s) 687
BspT107I GGYRCC 2 cut(s) 131, 874
BsrDI GCAATG 2 cut(s) 388, 531
BsrFI RCCGGY 2 cut(s) 576, 615
BsrI ACTGG 3 cut(s) 119, 268, 481
BssAI RCCGGY 2 cut(s) 576, 615
BssECI CCNNGG 6 cut(s) 600, 713, 826, 854, 981, 1135
BssHII GCGCGC 1 cut(s) 708
BssMI GATC 2 cut(s) 635, 966
BssNI GRCGYC 5 cut(s) 79, 132, 375, 749, 875
BssSI CACGAG 1 cut(s) 893
BssT1I CCWWGG 1 cut(s) 1135
Bst2BI CACGAG 1 cut(s) 893
Bst2UI CCWGG 3 cut(s) 226, 821, 836
Bst4CI ACNGT 1 cut(s) 783
BstACI GRCGYC 5 cut(s) 79, 132, 375, 749, 875
BstC8I GCNNGC 7 cut(s) 6, 317, 321, 492, 687, 710, 747
BstDEI CTNAG 1 cut(s) 74
BstDSI CCRYGG 2 cut(s) 826, 1135
BstF5I GGATG 1 cut(s) 1170
BstFNI CGCG 6 cut(s) 8, 234, 393, 658, 710, 828
BstH2I RGCGCY 4 cut(s) 58, 135, 800, 878
BstHHI GCGC 9 cut(s) 57, 134, 357, 449, 710, 712, 799, 877, 946
BstKTI GATC 2 cut(s) 638, 969
BstMAI GTCTC 1 cut(s) 658
BstMBI GATC 2 cut(s) 635, 966
BstMCI CGRYCG 3 cut(s) 331, 457, 662
BstMWI GCNNNNNNNGC 9 cut(s) 78, 255, 412, 500, 803, 825, 886, 889, 1072
BstNI CCWGG 3 cut(s) 226, 821, 836
BstPAI GACNNNNGTC 1 cut(s) 167
BstSCI CCNGG 7 cut(s) 224, 456, 600, 819, 834, 980, 981
BstSFI CTRYAG 1 cut(s) 683
BstUI CGCG 6 cut(s) 8, 234, 393, 658, 710, 828
BstV1I GCAGC 8 cut(s) 44, 324, 369, 669, 691, 867, 876, 1084
BstV2I GAAGAC 2 cut(s) 58, 1123
BstXI CCANNNNNNTGG 1 cut(s) 745
BsuRI GGCC 9 cut(s) 319, 368, 480, 503, 588, 605, 627, 718, 831
BtgI CCRYGG 2 cut(s) 826, 1135
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 1 cut(s) 1170
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 1 cut(s) 422
BtuMI TCGCGA 1 cut(s) 393
Cac8I GCNNGC 7 cut(s) 6, 317, 321, 492, 687, 710, 747
CfoI GCGC 9 cut(s) 57, 134, 357, 449, 710, 712, 799, 877, 946
Cfr10I RCCGGY 2 cut(s) 576, 615
Cfr42I CCGCGG 1 cut(s) 829
Cfr9I CCCGGG 1 cut(s) 981
CseI GACGC 4 cut(s) 87, 349, 640, 738
CspAI ACCGGT 2 cut(s) 576, 615
CviAII CATG 2 cut(s) 124, 1136
DdeI CTNAG 1 cut(s) 74
DinI GGCGCC 2 cut(s) 133, 876
DpnI GATC 2 cut(s) 637, 968
DpnII GATC 2 cut(s) 635, 966
DriI GACNNNNNGTC 1 cut(s) 616
EaeI YGGCCR 2 cut(s) 586, 603
Eam1105I GACNNNNNGTC 1 cut(s) 616
Eco130I CCWWGG 1 cut(s) 1135
Eco47I GGWCC 7 cut(s) 460, 579, 823, 838, 858, 925, 1102
Eco57I CTGAAG 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 981
EcoO109I RGGNCCY 3 cut(s) 367, 502, 858
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 3 cut(s) 224, 819, 834
EcoT14I CCWWGG 1 cut(s) 1135
EgeI GGCGCC 2 cut(s) 133, 876
EheI GGCGCC 2 cut(s) 133, 876
ErhI CCWWGG 1 cut(s) 1135
Esp3I CGTCTC 1 cut(s) 658
FaeI CATG 2 cut(s) 127, 1139
FaiI YATR 3 cut(s) 125, 989, 1137
FalI AAGNNNNNCTT 2 cut(s) 1082, 1114
FaqI GGGAC 2 cut(s) 473, 938
FatI CATG 2 cut(s) 123, 1135
FauI CCCGC 3 cut(s) 13, 328, 915
FblI GTMKAC 1 cut(s) 327
FokI GGATG 1 cut(s) 1177
FspBI CTAG 1 cut(s) 530
GlaI GCGC 9 cut(s) 56, 133, 356, 448, 709, 711, 798, 876, 945
GsaI CCCAGC 1 cut(s) 39
GsuI CTGGAG 1 cut(s) 818
HaeII RGCGCY 4 cut(s) 58, 135, 800, 878
HaeIII GGCC 9 cut(s) 319, 368, 480, 503, 588, 605, 627, 718, 831
HapII CCGG 9 cut(s) 457, 463, 487, 577, 589, 602, 616, 928, 982
HgaI GACGC 4 cut(s) 87, 349, 640, 738
HhaI GCGC 9 cut(s) 57, 134, 357, 449, 710, 712, 799, 877, 946
Hin1I GRCGYC 5 cut(s) 79, 132, 375, 749, 875
Hin1II CATG 2 cut(s) 127, 1139
Hin6I GCGC 9 cut(s) 55, 132, 355, 447, 708, 710, 797, 875, 944
HinP1I GCGC 9 cut(s) 55, 132, 355, 447, 708, 710, 797, 875, 944
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 8 cut(s) 388, 466, 811, 850, 911, 958, 1004, 1169
HpaII CCGG 9 cut(s) 457, 463, 487, 577, 589, 602, 616, 928, 982
HphI GGTGA 2 cut(s) 254, 754
Hpy166II GTNNAC 3 cut(s) 328, 762, 841
Hpy188I TCNGA 5 cut(s) 77, 343, 635, 855, 966
Hpy188III TCNNGA 9 cut(s) 143, 160, 392, 452, 847, 949, 970, 1061, 1109
Hpy8I GTNNAC 3 cut(s) 328, 762, 841
Hpy99I CGWCG 8 cut(s) 13, 31, 173, 248, 329, 377, 398, 754
HpyAV CCTTC 6 cut(s) 166, 470, 493, 871, 946, 1031
HpyCH4III ACNGT 1 cut(s) 783
HpyCH4IV ACGT 3 cut(s) 168, 306, 375
HpyCH4V TGCA 4 cut(s) 381, 524, 685, 892
HpyF10VI GCNNNNNNNGC 9 cut(s) 78, 255, 412, 500, 803, 825, 886, 889, 1072
HpyF3I CTNAG 1 cut(s) 74
HpySE526I ACGT 3 cut(s) 168, 306, 375
Hsp92I GRCGYC 5 cut(s) 79, 132, 375, 749, 875
Hsp92II CATG 2 cut(s) 127, 1139
HspAI GCGC 9 cut(s) 55, 132, 355, 447, 708, 710, 797, 875, 944
KasI GGCGCC 2 cut(s) 131, 874
KspI CCGCGG 1 cut(s) 829
Kzo9I GATC 2 cut(s) 635, 966
LmnI GCTCC 8 cut(s) 62, 234, 282, 551, 591, 648, 1063, 1149
Lsp1109I GCAGC 8 cut(s) 44, 324, 369, 669, 691, 867, 876, 1084
MaeI CTAG 1 cut(s) 530
MaeII ACGT 3 cut(s) 168, 306, 375
MalI GATC 2 cut(s) 637, 968
MboI GATC 2 cut(s) 635, 966
MboII GAAGA 2 cut(s) 58, 1123
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 5 cut(s) 14, 47, 769, 995, 1140
Mly113I GGCGCC 2 cut(s) 132, 875
MlyI GAGTC 3 cut(s) 460, 920, 1163
MmeI TCCRAC 3 cut(s) 208, 327, 573
MspA1I CMGCKG 4 cut(s) 35, 682, 828, 880
MspI CCGG 9 cut(s) 457, 463, 487, 577, 589, 602, 616, 928, 982
MspR9I CCNGG 7 cut(s) 226, 458, 602, 821, 836, 982, 983
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 3 cut(s) 226, 821, 836
MvnI CGCG 6 cut(s) 8, 234, 393, 658, 710, 828
MwoI GCNNNNNNNGC 9 cut(s) 78, 255, 412, 500, 803, 825, 886, 889, 1072
NarI GGCGCC 2 cut(s) 132, 875
NciI CCSGG 4 cut(s) 458, 602, 982, 983
NcoI CCATGG 1 cut(s) 1135
NdeII GATC 2 cut(s) 635, 966
NlaIII CATG 2 cut(s) 127, 1139
NlaIV GGNNCC 6 cut(s) 133, 230, 461, 876, 926, 1104
NmeAIII GCCGAG 2 cut(s) 450, 738
NruI TCGCGA 1 cut(s) 393
PauI GCGCGC 1 cut(s) 708
PcsI WCGNNNNNNNCGW 1 cut(s) 357
PctI GAATGC 1 cut(s) 258
PfeI GAWTC 5 cut(s) 388, 811, 850, 958, 1004
PinAI ACCGGT 2 cut(s) 576, 615
PleI GAGTC 3 cut(s) 460, 919, 1163
PluTI GGCGCC 2 cut(s) 135, 878
PpsI GAGTC 3 cut(s) 460, 919, 1163
PpuMI RGGWCCY 1 cut(s) 858
PshAI GACNNNNGTC 1 cut(s) 167
Psp5II RGGWCCY 1 cut(s) 858
Psp6I CCWGG 3 cut(s) 224, 819, 834
PspFI CCCAGC 1 cut(s) 35
PspGI CCWGG 3 cut(s) 224, 819, 834
PspN4I GGNNCC 6 cut(s) 133, 230, 461, 876, 926, 1104
PspPPI RGGWCCY 1 cut(s) 858
PstI CTGCAG 1 cut(s) 687
PteI GCGCGC 1 cut(s) 708
PvuII CAGCTG 2 cut(s) 35, 682
RruI TCGCGA 1 cut(s) 393
SacII CCGCGG 1 cut(s) 829
SalI GTCGAC 1 cut(s) 326
Sau3AI GATC 2 cut(s) 635, 966
SchI GAGTC 3 cut(s) 460, 920, 1163
ScrFI CCNGG 7 cut(s) 226, 458, 602, 821, 836, 982, 983
SduI GDGCHC 1 cut(s) 444
SfcI CTRYAG 1 cut(s) 683
SfoI GGCGCC 2 cut(s) 133, 876
Sfr303I CCGCGG 1 cut(s) 829
SgrBI CCGCGG 1 cut(s) 829
SinI GGWCC 7 cut(s) 460, 579, 823, 838, 858, 925, 1102
SmaI CCCGGG 1 cut(s) 983
Sse9I AATT 5 cut(s) 14, 47, 769, 995, 1140
SspDI GGCGCC 2 cut(s) 131, 874
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 7 cut(s) 224, 456, 600, 819, 834, 980, 981
StyI CCWWGG 1 cut(s) 1135
TaaI ACNGT 1 cut(s) 783
TaiI ACGT 3 cut(s) 171, 309, 378
TaqI TCGA 9 cut(s) 144, 150, 243, 327, 351, 372, 453, 914, 969
TasI AATT 5 cut(s) 14, 47, 769, 995, 1140
TauI GCSGC 7 cut(s) 84, 674, 809, 831, 875, 880, 922
TfiI GAWTC 5 cut(s) 388, 811, 850, 958, 1004
TscAI CASTG 1 cut(s) 422
TseI GCWGC 8 cut(s) 32, 312, 357, 679, 682, 880, 889, 1072
TspDTI ATGAA 2 cut(s) 738, 950
TspGWI ACGGA 4 cut(s) 26, 52, 804, 1008
TspMI CCCGGG 1 cut(s) 981
TspRI CASTG 1 cut(s) 422
VpaK11BI GGWCC 7 cut(s) 460, 579, 823, 838, 858, 925, 1102
XapI RAATTY 1 cut(s) 14
XcmI CCANNNNNNNNNTGG 1 cut(s) 727
XmaI CCCGGG 1 cut(s) 981
XmiI GTMKAC 1 cut(s) 327
XspI CTAG 1 cut(s) 530
ZraI GACGTC 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.