FvH4_2g13680

UPF0496 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010852
Physical Location & Seq
Reverse (-)
11948480 .. 11953074
4595 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g13680.t1

Sequence Viewer

Length: 1164 bp
ATGCCAGCGACGGAATTCCAAGGCTCCGCAGCAGCTCAATTCGGCCGCTCCGTGTTCAGCCTCAAGCGCCACAGCCACGTCACCCCAGTCGTGATGGACGGCGGCCAATCCCTCGACCTCGACACCTTCCAGAGCCACGTGTCCGATTGCTTCCAACAGCTCTCCTCCTCCTCGCACGACCTCCTCTCTCTTTCATGGCTCCGCGACCTCCTCGACTCTTTCCTCCGCATCCACCACCAGTTCAAGCTCCTCATCCTCTCCACCCCCAAGTCCCACCTCTCCAAGCCCACCGTCGACCGCCTCCTCTCCGACTACTTCGAGCGCAGCGTCAAGGGCCTCGACGTCTGTAATGCCATCCGCGACGGGATTGAGCAGATGCGCCAATGGCACAAGCTGTTAGAGATTGTTCTATGTGCTTTGGATCGTGACCGGACTCTCGGCGAAGGCCAGTTCCGGCGAGCCAAGAAAGCTCTCATTGACTTGGCTATTGCGATGCTTGACGACAAGGACAACGCCTCCAACAACACCACCCTCGCCCACCGCAACCGCTCCTTCGGCCGGAGCAACACGCCGGCCAAGGACTACCGGTCTGTCGGACACTTCCGATCTCTGTCGTGGAGCGTCTCGCGGTCGTGGTCGGCGGCCAGGCAGCTCCAGGCGATTGGGAACAACCTCTGCGCGCCGAGGCCCAACGAAATCGCCGCCACCAACGGGCTGGCCACGGCGGTGTTTACGGTCAATTCGGTTCTGCTGTTTGTAATGTGGGCCCTGGTGGCGGCGATTCCGTGCCAGGACCGCGGCCTGCAGGTCCACTTCACCATTCCGAGGACCTTCGGGTGGGCGGCGCCGCTGCTTTCGCTGCACGAGAGGATTATGGAGGAGTCGAAGCGCCGGGACCGGAGGAACGCCTGTGCGCTTTTGAAGGAGATTCATCAGATCGAGAAATGCTCCCGGCTCATCAGCGAATTGGCTGATTCCGTTCAGTTTCCGTTGAGTGAGGATAAAGAAGGGGAGGTCCGGCAGAGAGTTCAGGAGCTTTCGGAGCTCTGCGAGGGCATGAAGGAGGGTTTGGACCCTCTGGAAAGACAGGTCAGGGAGGTCTTCCATGGGATTGTGCGGAGCAGAACAGAAGGGATGGACTCTTTGGGAAAGACCAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BPS1

Protein Analysis

388

Amino Acids

43.67

Weight (kDa)

8.41

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 1 - 382 1e-148 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010852)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18740 AT1G74450
fragaria_vesca FvH4_2g13680 FvH4_2g13680
malus_domestica MD05G1026700.v1.1 MD10G1025900.v1.1
prunus_persica Prupe.8G031200_v2.0.a1
pyrus_communis pycom05g01850 pycom10g01740
rosa_chinensis RchiOBHm_Chr6g0275361
rosa_laevigata RLG00000013471
rosa_multiflora Rmu_co8417379.1_g000001 Rmu_sc0004143.1_g000001 Rmu_sc0019329.1_g000002
rosa_roxburghii Rroxscaffold_7G00193380
rosa_rugosa Rorug06G0091100
rosa_samantha Rh6AG205000 Rh6CG210200 Rh6DG200500
rosa_wichuraiana Rw6G017790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
Acc36I ACCTGC 1 cut(s) 796
AccB1I GGYRCC 1 cut(s) 844
AccBSI CCGCTC 2 cut(s) 48, 549
AccI GTMKAC 1 cut(s) 294
AccII CGCG 5 cut(s) 204, 360, 628, 680, 798
AclWI GGATC 1 cut(s) 429
AcoI YGGCCR 6 cut(s) 43, 103, 556, 573, 642, 717
AcsI RAATTY 1 cut(s) 14
AcvI CACGTG 1 cut(s) 139
AcyI GRCGYC 2 cut(s) 342, 845
AfiI CCNNNNNNNGG 5 cut(s) 558, 711, 775, 825, 837
AflIII ACRYGT 1 cut(s) 138
AgeI ACCGGT 1 cut(s) 585
AgsI TTSAA 2 cut(s) 244, 922
AhdI GACNNNNNGTC 1 cut(s) 586
AjiI CACGTC 1 cut(s) 79
AjnI CCWGG 4 cut(s) 644, 654, 768, 789
AjuI GAANNNNNNNTTGG 2 cut(s) 1052, 1084
AleI CACNNNNGTG 1 cut(s) 725
AluBI AGCT 8 cut(s) 35, 160, 247, 394, 470, 652, 1036, 1045
AluI AGCT 8 cut(s) 35, 160, 247, 394, 470, 652, 1036, 1045
Alw21I GWGCWC 1 cut(s) 1047
Alw26I GTCTC 1 cut(s) 628
AlwI GGATC 1 cut(s) 429
ApaI GGGCCC 1 cut(s) 769
ApeKI GCWGC 6 cut(s) 29, 32, 324, 649, 850, 859
ApoI RAATTY 1 cut(s) 14
AsiGI ACCGGT 1 cut(s) 585
AspLEI GCGC 8 cut(s) 69, 324, 381, 680, 682, 847, 891, 916
AsuC2I CCSGG 2 cut(s) 893, 952
AsuHPI GGTGA 2 cut(s) 73, 808
AvaII GGWCC 6 cut(s) 793, 808, 828, 895, 1015, 1072
BaeGI GKGCMC 1 cut(s) 769
BalI TGGCCA 1 cut(s) 719
BanI GGYRCC 1 cut(s) 844
BanII GRGCYC 2 cut(s) 769, 1047
BauI CACGAG 1 cut(s) 863
BbrPI CACGTG 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 1093
Bbv12I GWGCWC 1 cut(s) 1047
BbvI GCAGC 6 cut(s) 41, 44, 336, 661, 837, 846
BccI CCATC 3 cut(s) 88, 362, 1129
BceAI ACGGC 2 cut(s) 115, 738
BciT130I CCWGG 4 cut(s) 646, 656, 770, 791
BcnI CCSGG 2 cut(s) 893, 952
BcoDI GTCTC 1 cut(s) 628
BfmI CTRYAG 1 cut(s) 803
BfoI RGCGCY 3 cut(s) 70, 848, 892
BfuAI ACCTGC 1 cut(s) 796
BglI GCCNNNNNGGC 1 cut(s) 773
Bme1390I CCNGG 6 cut(s) 646, 656, 770, 791, 893, 952
Bme18I GGWCC 6 cut(s) 793, 808, 828, 895, 1015, 1072
BmeRI GACNNNNNGTC 1 cut(s) 586
BmgBI CACGTC 1 cut(s) 79
BmiI GGNNCC 6 cut(s) 25, 200, 767, 846, 896, 1074
BmrFI CCNGG 6 cut(s) 646, 656, 770, 791, 893, 952
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 3 cut(s) 237, 366, 483
BmuI ACTGGG 1 cut(s) 80
BpiI GAAGAC 1 cut(s) 1093
BplI GAGNNNNNCTC 2 cut(s) 932, 964
BpmI CTGGAG 1 cut(s) 638
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 2 cut(s) 893, 952
BsaAI YACGTR 1 cut(s) 139
BsaHI GRCGYC 2 cut(s) 342, 845
BsaJI CCNNGG 8 cut(s) 19, 576, 683, 720, 768, 796, 824, 1105
BsaWI WCCGGW 3 cut(s) 429, 585, 897
BsaXI ACNNNNNCTCC 2 cut(s) 500, 530
Bsc4I CCNNNNNNNGG 5 cut(s) 558, 711, 775, 825, 837
Bse118I RCCGGY 2 cut(s) 571, 585
Bse1I ACTGG 3 cut(s) 86, 238, 448
BseBI CCWGG 4 cut(s) 646, 656, 770, 791
BseDI CCNNGG 8 cut(s) 19, 576, 683, 720, 768, 796, 824, 1105
BseGI GGATG 4 cut(s) 228, 252, 354, 1140
BseLI CCNNNNNNNGG 5 cut(s) 558, 711, 775, 825, 837
BseNI ACTGG 3 cut(s) 86, 238, 448
BsePI GCGCGC 1 cut(s) 678
BseRI GAGGAG 8 cut(s) 154, 157, 160, 173, 200, 239, 293, 893
BseSI GKGCMC 1 cut(s) 769
BseX3I CGGCCG 2 cut(s) 43, 556
BseXI GCAGC 6 cut(s) 41, 44, 336, 661, 837, 846
BsgI GTGCAG 1 cut(s) 845
Bsh1236I CGCG 5 cut(s) 204, 360, 628, 680, 798
Bsh1285I CGRYCG 4 cut(s) 46, 298, 559, 632
BshNI GGYRCC 1 cut(s) 844
BshTI ACCGGT 1 cut(s) 585
BsiEI CGRYCG 4 cut(s) 46, 298, 559, 632
BsiHKAI GWGCWC 1 cut(s) 1047
BsiSI CCGG 9 cut(s) 430, 454, 559, 572, 586, 892, 898, 952, 1018
BslFI GGGAC 2 cut(s) 256, 908
BslI CCNNNNNNNGG 5 cut(s) 558, 711, 775, 825, 837
BsmAI GTCTC 1 cut(s) 628
BsmBI CGTCTC 1 cut(s) 628
BsmFI GGGAC 2 cut(s) 256, 908
Bsp120I GGGCCC 1 cut(s) 765
Bsp1286I GDGCHC 2 cut(s) 769, 1047
Bsp143I GATC 3 cut(s) 421, 605, 936
Bsp19I CCATGG 1 cut(s) 1105
BspFNI CGCG 5 cut(s) 204, 360, 628, 680, 798
BspLI GGNNCC 6 cut(s) 25, 200, 767, 846, 896, 1074
BspMAI CTGCAG 1 cut(s) 807
BspMI ACCTGC 1 cut(s) 796
BspPI GGATC 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 844
BsrBI CCGCTC 2 cut(s) 48, 549
BsrFI RCCGGY 2 cut(s) 571, 585
BsrI ACTGG 3 cut(s) 86, 238, 448
BssAI RCCGGY 2 cut(s) 571, 585
BssECI CCNNGG 8 cut(s) 19, 576, 683, 720, 768, 796, 824, 1105
BssHII GCGCGC 1 cut(s) 678
BssMI GATC 3 cut(s) 421, 605, 936
BssNI GRCGYC 2 cut(s) 342, 845
BssSI CACGAG 1 cut(s) 863
BssT1I CCWWGG 3 cut(s) 19, 576, 1105
Bst2BI CACGAG 1 cut(s) 863
Bst2UI CCWGG 4 cut(s) 646, 656, 770, 791
Bst4CI ACNGT 2 cut(s) 292, 736
BstACI GRCGYC 2 cut(s) 342, 845
BstBAI YACGTR 1 cut(s) 139
BstC8I GCNNGC 6 cut(s) 6, 459, 573, 680, 717, 803
BstDSI CCRYGG 3 cut(s) 720, 796, 1105
BstF5I GGATG 4 cut(s) 228, 252, 354, 1140
BstFNI CGCG 5 cut(s) 204, 360, 628, 680, 798
BstH2I RGCGCY 3 cut(s) 70, 848, 892
BstHHI GCGC 8 cut(s) 69, 324, 381, 680, 682, 847, 891, 916
BstKTI GATC 3 cut(s) 424, 608, 939
BstMAI GTCTC 1 cut(s) 628
BstMBI GATC 3 cut(s) 421, 605, 936
BstMCI CGRYCG 4 cut(s) 46, 298, 559, 632
BstNI CCWGG 4 cut(s) 646, 656, 770, 791
BstSCI CCNGG 6 cut(s) 644, 654, 768, 789, 891, 950
BstSFI CTRYAG 1 cut(s) 803
BstSLI GKGCMC 1 cut(s) 769
BstUI CGCG 5 cut(s) 204, 360, 628, 680, 798
BstV1I GCAGC 6 cut(s) 41, 44, 336, 661, 837, 846
BstV2I GAAGAC 1 cut(s) 1093
BstXI CCANNNNNNTGG 2 cut(s) 662, 715
BstZI CGGCCG 2 cut(s) 43, 556
BtgI CCRYGG 3 cut(s) 720, 796, 1105
BtgZI GCGATG 1 cut(s) 506
BtrI CACGTC 1 cut(s) 79
BtsCI GGATG 4 cut(s) 228, 252, 354, 1140
BveI ACCTGC 1 cut(s) 796
Cac8I GCNNGC 6 cut(s) 6, 459, 573, 680, 717, 803
CfoI GCGC 8 cut(s) 69, 324, 381, 680, 682, 847, 891, 916
Cfr10I RCCGGY 2 cut(s) 571, 585
Cfr42I CCGCGG 1 cut(s) 799
CseI GACGC 2 cut(s) 316, 610
CspAI ACCGGT 1 cut(s) 585
CviAII CATG 3 cut(s) 195, 1057, 1106
DinI GGCGCC 1 cut(s) 846
DpnI GATC 3 cut(s) 423, 607, 938
DpnII GATC 3 cut(s) 421, 605, 936
DriI GACNNNNNGTC 1 cut(s) 586
EaeI YGGCCR 6 cut(s) 43, 103, 556, 573, 642, 717
EagI CGGCCG 2 cut(s) 43, 556
Eam1105I GACNNNNNGTC 1 cut(s) 586
Ecl136II GAGCTC 1 cut(s) 1045
EclXI CGGCCG 2 cut(s) 43, 556
Eco130I CCWWGG 3 cut(s) 19, 576, 1105
Eco24I GRGCYC 2 cut(s) 769, 1047
Eco47I GGWCC 6 cut(s) 793, 808, 828, 895, 1015, 1072
Eco52I CGGCCG 2 cut(s) 43, 556
Eco53kI GAGCTC 1 cut(s) 1045
Eco72I CACGTG 1 cut(s) 139
EcoICRI GAGCTC 1 cut(s) 1045
EcoO109I RGGNCCY 3 cut(s) 334, 766, 828
EcoRI GAATTC 1 cut(s) 14
EcoRII CCWGG 4 cut(s) 644, 654, 768, 789
EcoT14I CCWWGG 3 cut(s) 19, 576, 1105
EcoT38I GRGCYC 2 cut(s) 769, 1047
EgeI GGCGCC 1 cut(s) 846
EheI GGCGCC 1 cut(s) 846
ErhI CCWWGG 3 cut(s) 19, 576, 1105
Esp3I CGTCTC 1 cut(s) 628
FaeI CATG 3 cut(s) 198, 1060, 1109
FaiI YATR 5 cut(s) 196, 412, 875, 1058, 1107
FaqI GGGAC 2 cut(s) 256, 908
FatI CATG 3 cut(s) 194, 1056, 1105
FblI GTMKAC 1 cut(s) 294
FokI GGATG 4 cut(s) 215, 239, 341, 1147
FriOI GRGCYC 2 cut(s) 769, 1047
GlaI GCGC 8 cut(s) 68, 323, 380, 679, 681, 846, 890, 915
GsuI CTGGAG 1 cut(s) 638
HaeII RGCGCY 3 cut(s) 70, 848, 892
HapII CCGG 9 cut(s) 430, 454, 559, 572, 586, 892, 898, 952, 1018
HgaI GACGC 2 cut(s) 316, 610
HhaI GCGC 8 cut(s) 69, 324, 381, 680, 682, 847, 891, 916
Hin1I GRCGYC 2 cut(s) 342, 845
Hin1II CATG 3 cut(s) 198, 1060, 1109
Hin6I GCGC 8 cut(s) 67, 322, 379, 678, 680, 845, 889, 914
HinP1I GCGC 8 cut(s) 67, 322, 379, 678, 680, 845, 889, 914
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 7 cut(s) 215, 433, 781, 881, 928, 974, 1139
HpaII CCGG 9 cut(s) 430, 454, 559, 572, 586, 892, 898, 952, 1018
HphI GGTGA 2 cut(s) 73, 808
Hpy166II GTNNAC 3 cut(s) 295, 732, 811
Hpy188I TCNGA 7 cut(s) 145, 310, 596, 605, 825, 936, 1042
Hpy188III TCNNGA 6 cut(s) 91, 130, 425, 940, 1031, 1079
Hpy8I GTNNAC 3 cut(s) 295, 732, 811
Hpy99I CGWCG 4 cut(s) 13, 296, 344, 365
HpyAV CCTTC 8 cut(s) 136, 437, 562, 841, 916, 1001, 1054, 1124
HpyCH4III ACNGT 2 cut(s) 292, 736
HpyCH4IV ACGT 3 cut(s) 78, 138, 342
HpyCH4V TGCA 2 cut(s) 805, 862
HpySE526I ACGT 3 cut(s) 78, 138, 342
Hsp92I GRCGYC 2 cut(s) 342, 845
Hsp92II CATG 3 cut(s) 198, 1060, 1109
HspAI GCGC 8 cut(s) 67, 322, 379, 678, 680, 845, 889, 914
KasI GGCGCC 1 cut(s) 844
KroI GCCGGC 1 cut(s) 571
KroNI GCCGGC 1 cut(s) 573
KspI CCGCGG 1 cut(s) 799
Kzo9I GATC 3 cut(s) 421, 605, 936
Lsp1109I GCAGC 6 cut(s) 41, 44, 336, 661, 837, 846
LweI GCATC 3 cut(s) 237, 366, 483
MaeII ACGT 3 cut(s) 78, 138, 342
MaeIII GTNAC 2 cut(s) 79, 425
MalI GATC 3 cut(s) 423, 607, 938
MbiI CCGCTC 2 cut(s) 48, 549
MboI GATC 3 cut(s) 421, 605, 936
MboII GAAGA 1 cut(s) 1093
MhlI GDGCHC 2 cut(s) 769, 1047
MlsI TGGCCA 1 cut(s) 719
MluCI AATT 4 cut(s) 14, 38, 739, 965
MluNI TGGCCA 1 cut(s) 719
Mly113I GGCGCC 1 cut(s) 845
MlyI GAGTC 4 cut(s) 209, 427, 890, 1133
MmeI TCCRAC 4 cut(s) 178, 333, 543, 574
Mox20I TGGCCA 1 cut(s) 719
MroNI GCCGGC 1 cut(s) 571
MscI TGGCCA 1 cut(s) 719
MslI CAYNNNNRTG 1 cut(s) 725
Msp20I TGGCCA 1 cut(s) 719
MspA1I CMGCKG 2 cut(s) 798, 850
MspI CCGG 9 cut(s) 430, 454, 559, 572, 586, 892, 898, 952, 1018
MspR9I CCNGG 6 cut(s) 646, 656, 770, 791, 893, 952
MvaI CCWGG 4 cut(s) 646, 656, 770, 791
MvnI CGCG 5 cut(s) 204, 360, 628, 680, 798
NaeI GCCGGC 1 cut(s) 573
NarI GGCGCC 1 cut(s) 845
NciI CCSGG 2 cut(s) 893, 952
NcoI CCATGG 1 cut(s) 1105
NdeII GATC 3 cut(s) 421, 605, 936
NgoMIV GCCGGC 1 cut(s) 571
NlaIII CATG 3 cut(s) 198, 1060, 1109
NlaIV GGNNCC 6 cut(s) 25, 200, 767, 846, 896, 1074
NmeAIII GCCGAG 2 cut(s) 417, 708
NmuCI GTSAC 2 cut(s) 79, 425
OliI CACNNNNGTG 1 cut(s) 725
PauI GCGCGC 1 cut(s) 678
PcsI WCGNNNNNNNCGW 2 cut(s) 48, 324
PdiI GCCGGC 1 cut(s) 573
PfeI GAWTC 3 cut(s) 781, 928, 974
PinAI ACCGGT 1 cut(s) 585
PleI GAGTC 4 cut(s) 209, 427, 889, 1133
PluTI GGCGCC 1 cut(s) 848
PmaCI CACGTG 1 cut(s) 139
PmlI CACGTG 1 cut(s) 139
PpsI GAGTC 4 cut(s) 209, 427, 889, 1133
Ppu21I YACGTR 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 828
Psp124BI GAGCTC 1 cut(s) 1047
Psp5II RGGWCCY 1 cut(s) 828
Psp6I CCWGG 4 cut(s) 644, 654, 768, 789
PspCI CACGTG 1 cut(s) 139
PspGI CCWGG 4 cut(s) 644, 654, 768, 789
PspN4I GGNNCC 6 cut(s) 25, 200, 767, 846, 896, 1074
PspOMI GGGCCC 1 cut(s) 765
PspPPI RGGWCCY 1 cut(s) 828
PstI CTGCAG 1 cut(s) 807
PteI GCGCGC 1 cut(s) 678
RseI CAYNNNNRTG 1 cut(s) 725
SacI GAGCTC 1 cut(s) 1047
SacII CCGCGG 1 cut(s) 799
SalI GTCGAC 1 cut(s) 293
Sau3AI GATC 3 cut(s) 421, 605, 936
SbfI CCTGCAGG 1 cut(s) 807
SchI GAGTC 4 cut(s) 209, 427, 890, 1133
ScrFI CCNGG 6 cut(s) 646, 656, 770, 791, 893, 952
SdaI CCTGCAGG 1 cut(s) 807
SduI GDGCHC 2 cut(s) 769, 1047
SfaNI GCATC 3 cut(s) 237, 366, 483
SfcI CTRYAG 1 cut(s) 803
SfoI GGCGCC 1 cut(s) 846
Sfr303I CCGCGG 1 cut(s) 799
SgrBI CCGCGG 1 cut(s) 799
SinI GGWCC 6 cut(s) 793, 808, 828, 895, 1015, 1072
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse8387I CCTGCAGG 1 cut(s) 807
Sse9I AATT 4 cut(s) 14, 38, 739, 965
SspDI GGCGCC 1 cut(s) 844
SstI GAGCTC 1 cut(s) 1047
StyD4I CCNGG 6 cut(s) 644, 654, 768, 789, 891, 950
StyI CCWWGG 3 cut(s) 19, 576, 1105
TaaI ACNGT 2 cut(s) 292, 736
TaiI ACGT 3 cut(s) 81, 141, 345
TaqI TCGA 8 cut(s) 114, 120, 213, 294, 318, 339, 884, 939
TasI AATT 4 cut(s) 14, 38, 739, 965
TauI GCSGC 8 cut(s) 48, 105, 644, 704, 779, 801, 845, 850
TfiI GAWTC 3 cut(s) 781, 928, 974
TseFI GTSAC 2 cut(s) 79, 425
TseI GCWGC 6 cut(s) 29, 32, 324, 649, 850, 859
Tsp45I GTSAC 2 cut(s) 79, 425
TspDTI ATGAA 3 cut(s) 183, 920, 1073
TspGWI ACGGA 5 cut(s) 26, 40, 774, 967, 978
VpaK11BI GGWCC 6 cut(s) 793, 808, 828, 895, 1015, 1072
XapI RAATTY 1 cut(s) 14
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
XmiI GTMKAC 1 cut(s) 294
ZraI GACGTC 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.