AT1G75460

ATP-dependent protease La (LON) domain protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008497
Physical Location & Seq
Forward (+)
28327698 .. 28329764
2067 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75460.1

Sequence Viewer

Length: 837 bp
ATGGCGCTTCCACACGTAGTCTCTTCACCTTCTTCAACTTCATTATCTCCTTCCTTCAAATCCAAACCACCGTTTCACTCCCTTAGATCTTTATCACCGACGGTTGATAATCGCCGGCGTTGTAAACTCAACAGTTCCTTCCGATGTTCATCTTCTTCATTCTCCGAGAAGCATCACAACGCGAAATCTCCAAAATCCGATGATATCGTCGAGCTTCCTCTGTTTCCACTCCCACTCGTTCTCTTCCCCGGAGCAATCCTTCCTCTTCAGATCTTCGAATTCCGTTACCGTATCATGATGCACACGCTCCTCCAATCTGATCTACGATTCGGCGTAGTTTACTCCGATTCCGTCTCTGGCTCCGCCGCAGAAGTAGGATGTGTAGGAGAAGTCGTGAAACACGAGCGTCTCGTCGATGATCGATTCTTTCTAGTCTGCAAAGGTCAAGAACGATTCCGAGTCACGAATGTCGTTCGTACGAAACCCTACCTTGTCGGAGAAGTCACGTGGCTTGAAGATCGTCCTTCCGGTGAAGAAAATCTTGATTCGTTAGCTAATGAAGTCGAAGTTCTAATGAAAGAAGTGATTAGGTTATCGAATAGATTGAATGGTAAGGCTGAGAAAGAAGTTCAAGATCTGAGGAGGAATCAGTTTCCGACGCCGTTTTCGTTCTTCGTCGGAAGTACGTTTGAAGGAGCTCCTAGAGAGCAGCAAGCGTTGCTTGAGCTTGAAGATACGGCAGCTAGGTTAAAAAGAGAAAGAGAGACTTTGAGGAATACACTTAATTATTTGACTGCAGCTTCTGCTGTTAAAGATGTGTTTCCTTCATCATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

278

Amino Acids

31.34

Weight (kDa)

8.66

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 71 - 257 1.2e-30 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 182
AciI CCGC 2 cut(s) 363, 366
AcsI RAATTY 1 cut(s) 278
AcuI CTGAAG 1 cut(s) 251
AcvI CACGTG 1 cut(s) 507
AcyI GRCGYC 1 cut(s) 659
AfaI GTAC 2 cut(s) 478, 685
AflIII ACRYGT 1 cut(s) 13
AgsI TTSAA 7 cut(s) 36, 58, 515, 607, 632, 692, 731
AluBI AGCT 6 cut(s) 214, 554, 698, 727, 743, 800
AluI AGCT 6 cut(s) 214, 554, 698, 727, 743, 800
Alw21I GWGCWC 1 cut(s) 700
Alw26I GTCTC 4 cut(s) 25, 358, 413, 758
AlwNI CAGNNNCTG 1 cut(s) 803
ApeKI GCWGC 3 cut(s) 709, 740, 797
ApoI RAATTY 1 cut(s) 278
ArsI GACNNNNNNTTYG 2 cut(s) 649, 681
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 249
AsuHPI GGTGA 3 cut(s) 18, 87, 542
AsuII TTCGAA 1 cut(s) 276
BanII GRGCYC 1 cut(s) 700
BauI CACGAG 1 cut(s) 401
BbrPI CACGTG 1 cut(s) 507
Bbv12I GWGCWC 1 cut(s) 700
BbvI GCAGC 3 cut(s) 721, 752, 809
BceAI ACGGC 2 cut(s) 646, 753
BcnI CCSGG 1 cut(s) 249
BcoDI GTCTC 4 cut(s) 25, 358, 413, 758
BfaI CTAG 3 cut(s) 431, 702, 744
BfmI CTRYAG 1 cut(s) 795
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 3 cut(s) 86, 270, 634
BisI GCNGC 4 cut(s) 366, 710, 741, 798
BlsI GCNGC 4 cut(s) 367, 711, 742, 799
Bme1390I CCNGG 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 361
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 181, 288
Bpu14I TTCGAA 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 743
BpuMI CCSGG 1 cut(s) 249
Bsa29I ATCGAT 1 cut(s) 421
BsaAI YACGTR 2 cut(s) 16, 507
BsaBI GATNNNNATC 2 cut(s) 91, 148
BsaHI GRCGYC 1 cut(s) 659
BsaJI CCNNGG 1 cut(s) 247
BsaWI WCCGGW 1 cut(s) 527
BsaXI ACNNNNNCTCC 2 cut(s) 378, 408
Bse118I RCCGGY 1 cut(s) 114
Bse8I GATNNNNATC 2 cut(s) 91, 148
BseCI ATCGAT 1 cut(s) 421
BseDI CCNNGG 1 cut(s) 247
BseGI GGATG 1 cut(s) 383
BseJI GATNNNNATC 2 cut(s) 91, 148
BseMII CTCAG 2 cut(s) 609, 629
BseRI GAGGAG 2 cut(s) 299, 655
BseXI GCAGC 3 cut(s) 721, 752, 809
Bsh1236I CGCG 1 cut(s) 182
BshVI ATCGAT 1 cut(s) 421
BsiHKAI GWGCWC 1 cut(s) 700
BsiSI CCGG 3 cut(s) 115, 249, 528
BsiWI CGTACG 1 cut(s) 476
BsmAI GTCTC 4 cut(s) 25, 358, 413, 758
BsmBI CGTCTC 2 cut(s) 358, 413
Bsp119I TTCGAA 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 700
Bsp143I GATC 6 cut(s) 86, 270, 319, 418, 517, 634
BspACI CCGC 2 cut(s) 363, 366
BspCNI CTCAG 2 cut(s) 610, 630
BspDI ATCGAT 1 cut(s) 421
BspFNI CGCG 1 cut(s) 182
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 361
BspMAI CTGCAG 1 cut(s) 799
BspT104I TTCGAA 1 cut(s) 276
BsrFI RCCGGY 1 cut(s) 114
BssAI RCCGGY 1 cut(s) 114
BssECI CCNNGG 1 cut(s) 247
BssMI GATC 6 cut(s) 86, 270, 319, 418, 517, 634
BssNI GRCGYC 1 cut(s) 659
BssSI CACGAG 1 cut(s) 401
Bst2BI CACGAG 1 cut(s) 401
Bst4CI ACNGT 4 cut(s) 72, 103, 134, 290
Bst6I CTCTTC 3 cut(s) 28, 248, 270
BstACI GRCGYC 1 cut(s) 659
BstAPI GCANNNNNTGC 2 cut(s) 718, 803
BstBAI YACGTR 2 cut(s) 16, 507
BstBI TTCGAA 1 cut(s) 276
BstC8I GCNNGC 2 cut(s) 116, 714
BstDEI CTNAG 4 cut(s) 83, 618, 638, 834
BstF5I GGATG 1 cut(s) 383
BstFNI CGCG 1 cut(s) 182
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 6 cut(s) 89, 273, 322, 421, 520, 637
BstMAI GTCTC 4 cut(s) 25, 358, 413, 758
BstMBI GATC 6 cut(s) 86, 270, 319, 418, 517, 634
BstMWI GCNNNNNNNGC 2 cut(s) 718, 803
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 1 cut(s) 795
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 3 cut(s) 721, 752, 809
BstX2I RGATCY 3 cut(s) 86, 270, 634
BstYI RGATCY 3 cut(s) 86, 270, 634
Bsu15I ATCGAT 1 cut(s) 421
BsuTUI ATCGAT 1 cut(s) 421
BtsCI GGATG 1 cut(s) 383
Cac8I GCNNGC 2 cut(s) 116, 714
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 1 cut(s) 294
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 114
ClaI ATCGAT 1 cut(s) 421
CseI GACGC 2 cut(s) 395, 667
Csp6I GTAC 2 cut(s) 477, 684
CviAII CATG 1 cut(s) 295
CviJI RGCY 9 cut(s) 214, 360, 511, 554, 617, 698, 727, 743, 800
CviKI_1 RGCY 9 cut(s) 214, 360, 511, 554, 617, 698, 727, 743, 800
CviQI GTAC 2 cut(s) 477, 684
DdeI CTNAG 4 cut(s) 83, 618, 638, 834
DpnI GATC 6 cut(s) 88, 272, 321, 420, 519, 636
DpnII GATC 6 cut(s) 86, 270, 319, 418, 517, 634
Eam1104I CTCTTC 3 cut(s) 28, 248, 270
EarI CTCTTC 3 cut(s) 28, 248, 270
EciI GGCGGA 1 cut(s) 352
Ecl136II GAGCTC 1 cut(s) 698
Eco24I GRGCYC 1 cut(s) 700
Eco32I GATATC 1 cut(s) 205
Eco53kI GAGCTC 1 cut(s) 698
Eco57I CTGAAG 1 cut(s) 251
Eco72I CACGTG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 278
EcoRV GATATC 1 cut(s) 205
EcoT38I GRGCYC 1 cut(s) 700
Esp3I CGTCTC 2 cut(s) 358, 413
FaeI CATG 1 cut(s) 298
FaiI YATR 1 cut(s) 296
FalI AAGNNNNNCTT 6 cut(s) 525, 557, 705, 737, 751, 783
FatI CATG 1 cut(s) 294
Fnu4HI GCNGC 4 cut(s) 366, 710, 741, 798
FokI GGATG 1 cut(s) 390
FriOI GRGCYC 1 cut(s) 700
Fsp4HI GCNGC 4 cut(s) 366, 710, 741, 798
FspBI CTAG 3 cut(s) 431, 702, 744
GlaI GCGC 1 cut(s) 6
GluI GCNGC 4 cut(s) 366, 710, 741, 798
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 3 cut(s) 115, 249, 528
HgaI GACGC 2 cut(s) 395, 667
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 659
Hin1II CATG 1 cut(s) 298
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 7 cut(s) 327, 347, 423, 453, 459, 545, 646
HpaII CCGG 3 cut(s) 115, 249, 528
HphI GGTGA 3 cut(s) 18, 87, 542
Hpy166II GTNNAC 2 cut(s) 125, 340
Hpy188III TCNNGA 6 cut(s) 295, 394, 446, 463, 542, 632
Hpy8I GTNNAC 2 cut(s) 125, 340
Hpy99I CGWCG 5 cut(s) 103, 212, 416, 661, 680
HpyAV CCTTC 8 cut(s) 39, 60, 64, 148, 269, 534, 686, 834
HpyCH4III ACNGT 4 cut(s) 72, 103, 134, 290
HpyCH4IV ACGT 3 cut(s) 15, 506, 686
HpyCH4V TGCA 3 cut(s) 301, 438, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 718, 803
HpyF3I CTNAG 4 cut(s) 83, 618, 638, 834
HpySE526I ACGT 3 cut(s) 15, 506, 686
Hsp92I GRCGYC 1 cut(s) 659
Hsp92II CATG 1 cut(s) 298
HspAI GCGC 1 cut(s) 5
KroI GCCGGC 1 cut(s) 114
KroNI GCCGGC 1 cut(s) 116
Kzo9I GATC 6 cut(s) 86, 270, 319, 418, 517, 634
LmnI GCTCC 5 cut(s) 251, 312, 365, 695, 703
LpnPI CCDG 4 cut(s) 128, 262, 342, 541
Lsp1109I GCAGC 3 cut(s) 721, 752, 809
LweI GCATC 2 cut(s) 181, 288
MaeI CTAG 3 cut(s) 431, 702, 744
MaeII ACGT 3 cut(s) 15, 506, 686
MaeIII GTNAC 3 cut(s) 284, 460, 502
MalI GATC 6 cut(s) 88, 272, 321, 420, 519, 636
MboI GATC 6 cut(s) 86, 270, 319, 418, 517, 634
MflI RGATCY 3 cut(s) 86, 270, 634
MhlI GDGCHC 1 cut(s) 700
MluCI AATT 2 cut(s) 278, 784
MlyI GAGTC 1 cut(s) 468
MmeI TCCRAC 3 cut(s) 475, 658, 680
MnlI CCTC 6 cut(s) 228, 273, 320, 633, 636, 765
MreI CGCCGGCG 1 cut(s) 114
MroNI GCCGGC 1 cut(s) 114
MseI TTAA 3 cut(s) 749, 783, 810
MspI CCGG 3 cut(s) 115, 249, 528
MspR9I CCNGG 1 cut(s) 249
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 2 cut(s) 718, 803
NaeI GCCGGC 1 cut(s) 116
NciI CCSGG 1 cut(s) 249
NdeII GATC 6 cut(s) 86, 270, 319, 418, 517, 634
NgoMIV GCCGGC 1 cut(s) 114
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 1 cut(s) 361
NmuCI GTSAC 2 cut(s) 460, 502
NspV TTCGAA 1 cut(s) 276
PagI TCATGA 1 cut(s) 294
PcsI WCGNNNNNNNCGW 3 cut(s) 399, 408, 665
PdiI GCCGGC 1 cut(s) 116
PfeI GAWTC 6 cut(s) 327, 347, 423, 453, 545, 646
Pfl23II CGTACG 1 cut(s) 476
PkrI GCNGC 4 cut(s) 367, 711, 742, 799
PleI GAGTC 1 cut(s) 467
PmaCI CACGTG 1 cut(s) 507
PmlI CACGTG 1 cut(s) 507
PpsI GAGTC 1 cut(s) 467
Ppu21I YACGTR 2 cut(s) 16, 507
Psp124BI GAGCTC 1 cut(s) 700
PspCI CACGTG 1 cut(s) 507
PspLI CGTACG 1 cut(s) 476
PspN4I GGNNCC 1 cut(s) 361
PstI CTGCAG 1 cut(s) 799
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 3 cut(s) 86, 270, 634
RsaI GTAC 2 cut(s) 478, 685
RsaNI GTAC 2 cut(s) 477, 684
SacI GAGCTC 1 cut(s) 700
SaqAI TTAA 3 cut(s) 749, 783, 810
SatI GCNGC 4 cut(s) 366, 710, 741, 798
Sau3AI GATC 6 cut(s) 86, 270, 319, 418, 517, 634
SchI GAGTC 1 cut(s) 468
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 1 cut(s) 700
SfaNI GCATC 2 cut(s) 181, 288
SfcI CTRYAG 1 cut(s) 795
SfuI TTCGAA 1 cut(s) 276
SgrAI CRCCGGYG 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
Sse9I AATT 2 cut(s) 278, 784
SsiI CCGC 2 cut(s) 363, 366
SspMI CTAG 3 cut(s) 431, 702, 744
SstI GAGCTC 1 cut(s) 700
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 4 cut(s) 72, 103, 134, 290
TaiI ACGT 3 cut(s) 18, 509, 689
TaqI TCGA 6 cut(s) 210, 276, 414, 421, 564, 596
TasI AATT 2 cut(s) 278, 784
TauI GCSGC 1 cut(s) 368
TfiI GAWTC 6 cut(s) 327, 347, 423, 453, 545, 646
Tru1I TTAA 3 cut(s) 749, 783, 810
Tru9I TTAA 3 cut(s) 749, 783, 810
TseFI GTSAC 2 cut(s) 460, 502
TseI GCWGC 3 cut(s) 709, 740, 797
Tsp45I GTSAC 2 cut(s) 460, 502
TspDTI ATGAA 6 cut(s) 30, 138, 147, 573, 590, 816
TspGWI ACGGA 2 cut(s) 272, 340
XapI RAATTY 1 cut(s) 278
XspI CTAG 3 cut(s) 431, 702, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.