Rh6DG422800

LON peptidase N-terminal domain and RING finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0008497
Physical Location & Seq
Forward (+)
60410887 .. 60411441
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG422800.1

Sequence Viewer

Length: 555 bp
ATGATGATGCACTCGCTCCTCCAGACCGATCTCCGCTTCGGCGTCATCTACAGCGACGCCGTCTCCGGCACTGCCGACGTCGGCTGCGTCGGCGAGGTCGTCAAGCACGAGCGGCTCGTCGACGACCGGTTCTTCCTGATTTGTAAAGGTCAGGAGCGTTTTAGGGTTACGGACCTGGTCCGGACCAAGCCCTACCTGGTGGCGGAGGTCAAGTGGCTGGAGGACCGGCCGTCCGGCGACGGCGAGGAGGACCTGGACACATTGGCCACCGAGGTTGAGTCATACATGAAGGACGTGATTCGGCTCTCCAACCGGCTCGGCGGCAAGCCGGAGAAGGAGGTGCAGGACCTGAGGCGGAACCTATTTCCGACGCCGTTCTCGTTCTTCGTCGGAAGCACTTTCGAAGGCGCGCCGAGGGAGCAGCAGGCCTTGCTGGAGTTGGAGGACACGGCGGCGAGGCTGAAGAGGGAGAAGGAGACGCTGAGGAACACGCTCAACTACTTGACGGCGGCGTCCGCTGTTAAAGACGTCTTTCCGTCGTCGTCGTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

184

Amino Acids

20.78

Weight (kDa)

5.1

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 24 - 161 2.2e-19 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 511
AatII GACGTC 2 cut(s) 81, 531
AccBSI CCGCTC 1 cut(s) 112
AccI GTMKAC 1 cut(s) 120
AccII CGCG 1 cut(s) 410
AccIII TCCGGA 1 cut(s) 180
AciI CCGC 8 cut(s) 34, 112, 203, 321, 355, 452, 509, 516
AcoI YGGCCR 2 cut(s) 227, 264
AcuI CTGAAG 1 cut(s) 482
AcyI GRCGYC 6 cut(s) 42, 57, 78, 371, 512, 528
AfiI CCNNNNNNNGG 1 cut(s) 202
AgeI ACCGGT 1 cut(s) 126
AhdI GACNNNNNGTC 1 cut(s) 229
AjiI CACGTC 1 cut(s) 295
AjnI CCWGG 3 cut(s) 174, 195, 252
Alw26I GTCTC 2 cut(s) 67, 470
AlwNI CAGNNNCTG 1 cut(s) 349
Aor13HI TCCGGA 1 cut(s) 180
AoxI GGCC 3 cut(s) 227, 264, 426
ApeKI GCWGC 2 cut(s) 84, 421
AscI GGCGCGCC 1 cut(s) 408
AsiGI ACCGGT 1 cut(s) 126
AspLEI GCGC 2 cut(s) 410, 412
AspS9I GGNCC 6 cut(s) 172, 178, 183, 223, 250, 346
AsuII TTCGAA 1 cut(s) 402
AvaII GGWCC 6 cut(s) 172, 178, 183, 223, 250, 346
AxyI CCTNAGG 1 cut(s) 350
BalI TGGCCA 1 cut(s) 266
BauI CACGAG 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 540
BbvCI CCTCAGC 1 cut(s) 482
BbvI GCAGC 2 cut(s) 71, 433
BceAI ACGGC 6 cut(s) 44, 214, 256, 358, 465, 522
BciT130I CCWGG 3 cut(s) 176, 197, 254
BcoDI GTCTC 2 cut(s) 67, 470
BfmI CTRYAG 1 cut(s) 49
BisI GCNGC 6 cut(s) 85, 113, 322, 422, 453, 510
BlsI GCNGC 6 cut(s) 86, 114, 323, 423, 454, 511
Bme1390I CCNGG 3 cut(s) 176, 197, 254
Bme18I GGWCC 6 cut(s) 172, 178, 183, 223, 250, 346
BmeRI GACNNNNNGTC 1 cut(s) 229
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 6 cut(s) 172, 178, 183, 223, 250, 346
BmiI GGNNCC 1 cut(s) 359
BmrFI CCNGG 3 cut(s) 176, 197, 254
BpiI GAAGAC 1 cut(s) 540
BpmI CTGGAG 3 cut(s) 5, 239, 455
Bpu10I CCTNAGC 1 cut(s) 482
Bpu14I TTCGAA 1 cut(s) 402
BsaHI GRCGYC 6 cut(s) 42, 57, 78, 371, 512, 528
BsaJI CCNNGG 2 cut(s) 270, 413
BsaWI WCCGGW 2 cut(s) 126, 180
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 197, 227
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse118I RCCGGY 3 cut(s) 126, 225, 312
Bse21I CCTNAGG 1 cut(s) 350
BseAI TCCGGA 1 cut(s) 180
BseBI CCWGG 3 cut(s) 176, 197, 254
BseDI CCNNGG 2 cut(s) 270, 413
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMII CTCAG 2 cut(s) 341, 473
BsePI GCGCGC 1 cut(s) 408
BseRI GAGGAG 2 cut(s) 8, 260
BseX3I CGGCCG 1 cut(s) 227
BseXI GCAGC 2 cut(s) 71, 433
BsgI GTGCAG 1 cut(s) 362
Bsh1236I CGCG 1 cut(s) 410
Bsh1285I CGRYCG 2 cut(s) 127, 230
BshFI GGCC 3 cut(s) 229, 266, 428
BshTI ACCGGT 1 cut(s) 126
BsiEI CGRYCG 2 cut(s) 127, 230
BsiSI CCGG 7 cut(s) 66, 127, 181, 226, 234, 313, 329
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 2 cut(s) 67, 470
BsmBI CGTCTC 2 cut(s) 67, 470
BsnI GGCC 3 cut(s) 229, 266, 428
Bsp119I TTCGAA 1 cut(s) 402
Bsp13I TCCGGA 1 cut(s) 180
Bsp143I GATC 1 cut(s) 28
BspACI CCGC 8 cut(s) 34, 112, 203, 321, 355, 452, 509, 516
BspANI GGCC 3 cut(s) 229, 266, 428
BspCNI CTCAG 2 cut(s) 342, 474
BspEI TCCGGA 1 cut(s) 180
BspFNI CGCG 1 cut(s) 410
BspLI GGNNCC 1 cut(s) 359
BspT104I TTCGAA 1 cut(s) 402
BsrBI CCGCTC 1 cut(s) 112
BsrFI RCCGGY 3 cut(s) 126, 225, 312
BssAI RCCGGY 3 cut(s) 126, 225, 312
BssECI CCNNGG 2 cut(s) 270, 413
BssHII GCGCGC 1 cut(s) 408
BssMI GATC 1 cut(s) 28
BssNI GRCGYC 6 cut(s) 42, 57, 78, 371, 512, 528
BssSI CACGAG 1 cut(s) 107
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 3 cut(s) 176, 197, 254
Bst6I CTCTTC 1 cut(s) 458
BstACI GRCGYC 6 cut(s) 42, 57, 78, 371, 512, 528
BstAPI GCANNNNNTGC 1 cut(s) 430
BstBI TTCGAA 1 cut(s) 402
BstC8I GCNNGC 3 cut(s) 326, 410, 426
BstDEI CTNAG 2 cut(s) 350, 482
BstFNI CGCG 1 cut(s) 410
BstHHI GCGC 2 cut(s) 410, 412
BstKTI GATC 1 cut(s) 31
BstMAI GTCTC 2 cut(s) 67, 470
BstMBI GATC 1 cut(s) 28
BstMCI CGRYCG 2 cut(s) 127, 230
BstMWI GCNNNNNNNGC 5 cut(s) 90, 112, 418, 430, 515
BstNI CCWGG 3 cut(s) 176, 197, 254
BstSCI CCNGG 3 cut(s) 174, 195, 252
BstSFI CTRYAG 1 cut(s) 49
BstUI CGCG 1 cut(s) 410
BstV1I GCAGC 2 cut(s) 71, 433
BstV2I GAAGAC 1 cut(s) 540
BstZI CGGCCG 1 cut(s) 227
Bsu36I CCTNAGG 1 cut(s) 350
BsuRI GGCC 3 cut(s) 229, 266, 428
BtrI CACGTC 1 cut(s) 295
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 1 cut(s) 69
Cac8I GCNNGC 3 cut(s) 326, 410, 426
CaiI CAGNNNCTG 1 cut(s) 349
CfoI GCGC 2 cut(s) 410, 412
Cfr10I RCCGGY 3 cut(s) 126, 225, 312
Cfr13I GGNCC 6 cut(s) 172, 178, 183, 223, 250, 346
CseI GACGC 6 cut(s) 31, 65, 76, 379, 487, 501
CsiI ACCWGGT 2 cut(s) 174, 195
CspAI ACCGGT 1 cut(s) 126
CspCI CAANNNNNGTGG 2 cut(s) 256, 291
CviAII CATG 1 cut(s) 286
DdeI CTNAG 2 cut(s) 350, 482
DpnI GATC 1 cut(s) 30
DpnII GATC 1 cut(s) 28
DrdI GACNNNNNNGTC 1 cut(s) 511
DriI GACNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 2 cut(s) 227, 264
EagI CGGCCG 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 458
Eam1105I GACNNNNNGTC 1 cut(s) 229
EarI CTCTTC 1 cut(s) 458
EciI GGCGGA 2 cut(s) 218, 370
EclXI CGGCCG 1 cut(s) 227
Eco147I AGGCCT 1 cut(s) 428
Eco47I GGWCC 6 cut(s) 172, 178, 183, 223, 250, 346
Eco52I CGGCCG 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 482
Eco81I CCTNAGG 1 cut(s) 350
EcoO109I RGGNCCY 2 cut(s) 250, 346
EcoRII CCWGG 3 cut(s) 174, 195, 252
Esp3I CGTCTC 2 cut(s) 67, 470
FaeI CATG 1 cut(s) 289
FaiI YATR 2 cut(s) 283, 287
FatI CATG 1 cut(s) 285
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 6 cut(s) 85, 113, 322, 422, 453, 510
Fsp4HI GCNGC 6 cut(s) 85, 113, 322, 422, 453, 510
GlaI GCGC 2 cut(s) 409, 411
GluI GCNGC 6 cut(s) 85, 113, 322, 422, 453, 510
GsuI CTGGAG 3 cut(s) 5, 239, 455
HaeIII GGCC 3 cut(s) 229, 266, 428
HapII CCGG 7 cut(s) 66, 127, 181, 226, 234, 313, 329
HgaI GACGC 6 cut(s) 31, 65, 76, 379, 487, 501
HhaI GCGC 2 cut(s) 410, 412
Hin1I GRCGYC 6 cut(s) 42, 57, 78, 371, 512, 528
Hin1II CATG 1 cut(s) 289
Hin6I GCGC 2 cut(s) 408, 410
HinP1I GCGC 2 cut(s) 408, 410
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 2 cut(s) 278, 298
HpaII CCGG 7 cut(s) 66, 127, 181, 226, 234, 313, 329
Hpy166II GTNNAC 1 cut(s) 121
Hpy188I TCNGA 2 cut(s) 369, 392
Hpy188III TCNNGA 5 cut(s) 22, 136, 152, 181, 552
Hpy8I GTNNAC 1 cut(s) 121
HpyAV CCTTC 4 cut(s) 283, 328, 398, 466
HpyCH4IV ACGT 3 cut(s) 78, 294, 528
HpyCH4V TGCA 2 cut(s) 10, 343
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 112, 418, 430, 515
HpyF3I CTNAG 2 cut(s) 350, 482
HpySE526I ACGT 3 cut(s) 78, 294, 528
Hsp92I GRCGYC 6 cut(s) 42, 57, 78, 371, 512, 528
Hsp92II CATG 1 cut(s) 289
HspAI GCGC 2 cut(s) 408, 410
Kpn2I TCCGGA 1 cut(s) 180
Kzo9I GATC 1 cut(s) 28
LmnI GCTCC 3 cut(s) 21, 154, 418
Lsp1109I GCAGC 2 cut(s) 71, 433
MabI ACCWGGT 2 cut(s) 174, 195
MaeII ACGT 3 cut(s) 78, 294, 528
MaeIII GTNAC 1 cut(s) 166
MalI GATC 1 cut(s) 30
MbiI CCGCTC 1 cut(s) 112
MboI GATC 1 cut(s) 28
MboII GAAGA 4 cut(s) 124, 376, 475, 540
MlsI TGGCCA 1 cut(s) 266
MluNI TGGCCA 1 cut(s) 266
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 4 cut(s) 333, 370, 392, 420
Mox20I TGGCCA 1 cut(s) 266
MroI TCCGGA 1 cut(s) 180
MscI TGGCCA 1 cut(s) 266
MseI TTAA 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 266
MspA1I CMGCKG 1 cut(s) 518
MspI CCGG 7 cut(s) 66, 127, 181, 226, 234, 313, 329
MspR9I CCNGG 3 cut(s) 176, 197, 254
MvaI CCWGG 3 cut(s) 176, 197, 254
MvnI CGCG 1 cut(s) 410
MwoI GCNNNNNNNGC 5 cut(s) 90, 112, 418, 430, 515
NdeII GATC 1 cut(s) 28
NlaIII CATG 1 cut(s) 289
NlaIV GGNNCC 1 cut(s) 359
NmeAIII GCCGAG 2 cut(s) 297, 438
NspV TTCGAA 1 cut(s) 402
PalAI GGCGCGCC 1 cut(s) 408
PauI GCGCGC 1 cut(s) 408
PceI AGGCCT 1 cut(s) 428
PcsI WCGNNNNNNNCGW 5 cut(s) 84, 96, 105, 114, 377
PfeI GAWTC 1 cut(s) 298
PflFI GACNNNGTC 2 cut(s) 59, 176
PinAI ACCGGT 1 cut(s) 126
PkrI GCNGC 6 cut(s) 86, 114, 323, 423, 454, 511
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PpuMI RGGWCCY 2 cut(s) 250, 346
Psp5II RGGWCCY 2 cut(s) 250, 346
Psp6I CCWGG 3 cut(s) 174, 195, 252
PspGI CCWGG 3 cut(s) 174, 195, 252
PspN4I GGNNCC 1 cut(s) 359
PspPI GGNCC 6 cut(s) 172, 178, 183, 223, 250, 346
PspPPI RGGWCCY 2 cut(s) 250, 346
PstNI CAGNNNCTG 1 cut(s) 349
PsyI GACNNNGTC 2 cut(s) 59, 176
PteI GCGCGC 1 cut(s) 408
SalI GTCGAC 1 cut(s) 119
SaqAI TTAA 1 cut(s) 522
SatI GCNGC 6 cut(s) 85, 113, 322, 422, 453, 510
Sau3AI GATC 1 cut(s) 28
Sau96I GGNCC 6 cut(s) 172, 178, 183, 223, 250, 346
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 3 cut(s) 176, 197, 254
SexAI ACCWGGT 2 cut(s) 174, 195
SfcI CTRYAG 1 cut(s) 49
SfuI TTCGAA 1 cut(s) 402
SgrDI CGTCGACG 1 cut(s) 119
SgsI GGCGCGCC 1 cut(s) 408
SinI GGWCC 6 cut(s) 172, 178, 183, 223, 250, 346
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 8 cut(s) 34, 112, 203, 321, 355, 452, 509, 516
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 174, 195, 252
TaiI ACGT 3 cut(s) 81, 297, 531
TaqI TCGA 2 cut(s) 120, 402
TaqII GACCGA 1 cut(s) 41
TauI GCSGC 4 cut(s) 115, 324, 455, 512
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 1 cut(s) 76
TseI GCWGC 2 cut(s) 84, 421
TspDTI ATGAA 1 cut(s) 302
TspGWI ACGGA 2 cut(s) 185, 525
TspRI CASTG 1 cut(s) 76
Tth111I GACNNNGTC 2 cut(s) 59, 176
VpaK11BI GGWCC 6 cut(s) 172, 178, 183, 223, 250, 346
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XmiI GTMKAC 1 cut(s) 120
ZraI GACGTC 2 cut(s) 79, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.