MD08G1023400.v1.1

LON peptidase N-terminal domain and RING finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008497
Physical Location & Seq
Reverse (-)
1688079 .. 1688924
846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1023400.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGCTCTACCTCAGCTCATCCCCTCCCCTTCCTCCTCCTCCCTCCCCCATAAACCCACCTTCAACCCCAATCCCATCCCCTCCCCCTTAAACCCGAACCCTAATTTCCTCTCCCCCCATCTCCGCCGCCGCCGCCAACGACCTGGCTCCCTCAGATGCTCCGCCTCCTCATTCTCTGAGCGCCACCCCACCAATCAGCCCAAATCAGACGACGTCGTCGAGCTCCCCATCTTCCCCCTCCCCCTCGTCCTCTTCCCCGGCGCCATCCTCCCACTTCAGATCTTCGAGTTCCGCTACCGTATCATGATGCACTCCCTCCTCCAGACCGATCTCCGATTCGGGGTTATCTACAATGATGCCGTCTCCGGCACCGCCGACGTTGGCTGCGTCGGCGAGGTCGTCAAGCACGAGCGCCTCGTCGACGACCGGTTCTTCCTTATCTGCAAAGGCCAGGAGCGGTTCCGGGTCACCGACCTCGTCCGTACCAAACCTTACCTGGTGGCCGAGGTCAAATGGCTCGAAGACCGGCCGTCGAACGACGGCGAGGAGGACCTGGAGGCGTTGGCCAGCGAGGTCGAGTCGTATATGAAGGATGTGATTCGGTTGTCAAATCGGTTGGGCGGGAAGCCGGAGAAGGATGTGCAGGACCTGCGGCGGAACCTGTTTCCGACGCCGTTTTCGTTTTTCGTTGGGAGCACTTTCGAAGGGGCGCCGAGAGAGCAGCAAGCGTTGCTGGAACTGGAGGACACGGCGGCGCGGCTTAAGAGAGAGAAGGAGACTCTGAGGAACACGCTTAATTACTTGACGGCGGCCTCCGCCGTCAAAGACGTCTTCCCGTCGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

282

Amino Acids

31.78

Weight (kDa)

6.98

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 74 - 261 3e-29 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AatII GACGTC 2 cut(s) 216, 831
Acc36I ACCTGC 1 cut(s) 657
AccB1I GGYRCC 3 cut(s) 260, 368, 709
AccBSI CCGCTC 1 cut(s) 457
AccI GTMKAC 1 cut(s) 420
AccII CGCG 1 cut(s) 757
AcoI YGGCCR 3 cut(s) 501, 527, 564
AcuI CTGAAG 1 cut(s) 260
AcyI GRCGYC 5 cut(s) 213, 261, 671, 710, 828
AfaI GTAC 1 cut(s) 484
AfiI CCNNNNNNNGG 1 cut(s) 340
AflII CTTAAG 1 cut(s) 761
AgeI ACCGGT 1 cut(s) 426
AgsI TTSAA 1 cut(s) 64
AhdI GACNNNNNGTC 1 cut(s) 529
AjnI CCWGG 4 cut(s) 142, 450, 495, 552
AluBI AGCT 2 cut(s) 16, 223
AluI AGCT 2 cut(s) 16, 223
Alw21I GWGCWC 2 cut(s) 225, 698
Alw26I GTCTC 2 cut(s) 367, 770
AlwNI CAGNNNCTG 1 cut(s) 649
AoxI GGCC 5 cut(s) 448, 501, 527, 564, 810
ApeKI GCWGC 2 cut(s) 384, 721
ArsI GACNNNNNNTTYG 2 cut(s) 661, 693
AsiGI ACCGGT 1 cut(s) 426
AspLEI GCGC 5 cut(s) 183, 263, 414, 712, 757
AspS9I GGNCC 2 cut(s) 550, 646
AsuC2I CCSGG 2 cut(s) 258, 464
AsuHPI GGTGA 1 cut(s) 460
AsuII TTCGAA 1 cut(s) 702
AvaII GGWCC 2 cut(s) 550, 646
BalI TGGCCA 1 cut(s) 566
BanI GGYRCC 3 cut(s) 260, 368, 709
BanII GRGCYC 1 cut(s) 225
BauI CACGAG 1 cut(s) 407
BbsI GAAGAC 2 cut(s) 528, 823
Bbv12I GWGCWC 2 cut(s) 225, 698
BbvCI CCTCAGC 1 cut(s) 12
BbvI GCAGC 2 cut(s) 371, 733
BccI CCATC 4 cut(s) 83, 126, 236, 272
BceAI ACGGC 7 cut(s) 344, 514, 556, 658, 765, 803, 822
BciT130I CCWGG 4 cut(s) 144, 452, 497, 554
BcnI CCSGG 2 cut(s) 258, 464
BcoDI GTCTC 2 cut(s) 367, 770
BfoI RGCGCY 4 cut(s) 184, 264, 415, 713
BfrI CTTAAG 1 cut(s) 761
BfuAI ACCTGC 1 cut(s) 657
BglII AGATCT 1 cut(s) 279
BisI GCNGC 9 cut(s) 127, 130, 133, 385, 653, 722, 753, 758, 810
BlsI GCNGC 9 cut(s) 128, 131, 134, 386, 654, 723, 754, 759, 811
Bme1390I CCNGG 6 cut(s) 144, 258, 452, 464, 497, 554
Bme18I GGWCC 2 cut(s) 550, 646
BmeRI GACNNNNNGTC 1 cut(s) 529
BmgT120I GGNCC 2 cut(s) 550, 646
BmiI GGNNCC 6 cut(s) 148, 262, 370, 461, 659, 711
BmrFI CCNGG 6 cut(s) 144, 258, 452, 464, 497, 554
BmsI GCATC 3 cut(s) 146, 297, 346
BpiI GAAGAC 2 cut(s) 528, 823
BpmI CTGGAG 3 cut(s) 305, 575, 761
Bpu10I CCTNAGC 1 cut(s) 12
Bpu14I TTCGAA 1 cut(s) 702
BpuMI CCSGG 2 cut(s) 258, 464
BsaHI GRCGYC 5 cut(s) 213, 261, 671, 710, 828
BsaJI CCNNGG 2 cut(s) 256, 504
BsaWI WCCGGW 1 cut(s) 426
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse118I RCCGGY 2 cut(s) 426, 525
Bse1I ACTGG 1 cut(s) 744
BseBI CCWGG 4 cut(s) 144, 452, 497, 554
BseDI CCNNGG 2 cut(s) 256, 504
BseGI GGATG 5 cut(s) 18, 75, 264, 598, 643
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMII CTCAG 4 cut(s) 26, 166, 168, 773
BseNI ACTGG 1 cut(s) 744
BseRI GAGGAG 5 cut(s) 25, 28, 157, 308, 560
BseX3I CGGCCG 1 cut(s) 527
BseXI GCAGC 2 cut(s) 371, 733
BsgI GTGCAG 1 cut(s) 662
Bsh1236I CGCG 1 cut(s) 757
Bsh1285I CGRYCG 2 cut(s) 427, 530
BshFI GGCC 5 cut(s) 450, 503, 529, 566, 812
BshNI GGYRCC 3 cut(s) 260, 368, 709
BshTI ACCGGT 1 cut(s) 426
BsiEI CGRYCG 2 cut(s) 427, 530
BsiHKAI GWGCWC 2 cut(s) 225, 698
BsiSI CCGG 6 cut(s) 258, 366, 427, 463, 526, 629
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 2 cut(s) 367, 770
BsmBI CGTCTC 1 cut(s) 367
BsnI GGCC 5 cut(s) 450, 503, 529, 566, 812
Bsp119I TTCGAA 1 cut(s) 702
Bsp1286I GDGCHC 2 cut(s) 225, 698
Bsp143I GATC 2 cut(s) 279, 328
BspANI GGCC 5 cut(s) 450, 503, 529, 566, 812
BspCNI CTCAG 4 cut(s) 25, 165, 169, 774
BspFNI CGCG 1 cut(s) 757
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 6 cut(s) 148, 262, 370, 461, 659, 711
BspMI ACCTGC 1 cut(s) 657
BspT104I TTCGAA 1 cut(s) 702
BspT107I GGYRCC 3 cut(s) 260, 368, 709
BspTI CTTAAG 1 cut(s) 761
BsrBI CCGCTC 1 cut(s) 457
BsrFI RCCGGY 2 cut(s) 426, 525
BsrI ACTGG 1 cut(s) 744
BssAI RCCGGY 2 cut(s) 426, 525
BssECI CCNNGG 2 cut(s) 256, 504
BssMI GATC 2 cut(s) 279, 328
BssNI GRCGYC 5 cut(s) 213, 261, 671, 710, 828
BssSI CACGAG 1 cut(s) 407
Bst2BI CACGAG 1 cut(s) 407
Bst2UI CCWGG 4 cut(s) 144, 452, 497, 554
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 1 cut(s) 257
BstACI GRCGYC 5 cut(s) 213, 261, 671, 710, 828
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 2 cut(s) 649, 730
BstBI TTCGAA 1 cut(s) 702
BstC8I GCNNGC 2 cut(s) 568, 726
BstDEI CTNAG 4 cut(s) 12, 152, 177, 782
BstEII GGTNACC 1 cut(s) 466
BstF5I GGATG 5 cut(s) 18, 75, 264, 598, 643
BstFNI CGCG 1 cut(s) 757
BstH2I RGCGCY 4 cut(s) 184, 264, 415, 713
BstHHI GCGC 5 cut(s) 183, 263, 414, 712, 757
BstKTI GATC 2 cut(s) 282, 331
BstMAI GTCTC 2 cut(s) 367, 770
BstMBI GATC 2 cut(s) 279, 328
BstMCI CGRYCG 2 cut(s) 427, 530
BstMWI GCNNNNNNNGC 6 cut(s) 132, 390, 649, 718, 730, 815
BstNI CCWGG 4 cut(s) 144, 452, 497, 554
BstPI GGTNACC 1 cut(s) 466
BstSCI CCNGG 6 cut(s) 142, 256, 450, 462, 495, 552
BstUI CGCG 1 cut(s) 757
BstV1I GCAGC 2 cut(s) 371, 733
BstV2I GAAGAC 2 cut(s) 528, 823
BstX2I RGATCY 1 cut(s) 279
BstXI CCANNNNNNTGG 1 cut(s) 143
BstYI RGATCY 1 cut(s) 279
BstZI CGGCCG 1 cut(s) 527
BsuRI GGCC 5 cut(s) 450, 503, 529, 566, 812
BtsCI GGATG 5 cut(s) 18, 75, 264, 598, 643
BveI ACCTGC 1 cut(s) 657
Cac8I GCNNGC 2 cut(s) 568, 726
CaiI CAGNNNCTG 1 cut(s) 649
CciI TCATGA 1 cut(s) 303
CfoI GCGC 5 cut(s) 183, 263, 414, 712, 757
Cfr10I RCCGGY 2 cut(s) 426, 525
Cfr13I GGNCC 2 cut(s) 550, 646
CseI GACGC 2 cut(s) 376, 679
CsiI ACCWGGT 1 cut(s) 495
Csp6I GTAC 1 cut(s) 483
CspAI ACCGGT 1 cut(s) 426
CviAII CATG 1 cut(s) 304
CviQI GTAC 1 cut(s) 483
DdeI CTNAG 4 cut(s) 12, 152, 177, 782
DinI GGCGCC 2 cut(s) 262, 711
DpnI GATC 2 cut(s) 281, 330
DpnII GATC 2 cut(s) 279, 328
DrdI GACNNNNNNGTC 1 cut(s) 215
DriI GACNNNNNGTC 1 cut(s) 529
DseDI GACNNNNNNGTC 1 cut(s) 215
EaeI YGGCCR 3 cut(s) 501, 527, 564
EagI CGGCCG 1 cut(s) 527
Eam1104I CTCTTC 1 cut(s) 257
Eam1105I GACNNNNNGTC 1 cut(s) 529
EarI CTCTTC 1 cut(s) 257
EciI GGCGGA 4 cut(s) 113, 151, 670, 805
Ecl136II GAGCTC 1 cut(s) 223
EclXI CGGCCG 1 cut(s) 527
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 2 cut(s) 550, 646
Eco52I CGGCCG 1 cut(s) 527
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 260
Eco91I GGTNACC 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 550, 646
EcoO65I GGTNACC 1 cut(s) 466
EcoRII CCWGG 4 cut(s) 142, 450, 495, 552
EcoT38I GRGCYC 1 cut(s) 225
EgeI GGCGCC 2 cut(s) 262, 711
EheI GGCGCC 2 cut(s) 262, 711
Esp3I CGTCTC 1 cut(s) 367
FaeI CATG 1 cut(s) 307
FaiI YATR 4 cut(s) 51, 305, 585, 587
FatI CATG 1 cut(s) 303
FauI CCCGC 1 cut(s) 614
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 9 cut(s) 127, 130, 133, 385, 653, 722, 753, 758, 810
FokI GGATG 5 cut(s) 5, 62, 251, 605, 650
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 9 cut(s) 127, 130, 133, 385, 653, 722, 753, 758, 810
GlaI GCGC 5 cut(s) 182, 262, 413, 711, 756
GluI GCNGC 9 cut(s) 127, 130, 133, 385, 653, 722, 753, 758, 810
GsuI CTGGAG 3 cut(s) 305, 575, 761
HaeII RGCGCY 4 cut(s) 184, 264, 415, 713
HaeIII GGCC 5 cut(s) 450, 503, 529, 566, 812
HapII CCGG 6 cut(s) 258, 366, 427, 463, 526, 629
HgaI GACGC 2 cut(s) 376, 679
HhaI GCGC 5 cut(s) 183, 263, 414, 712, 757
Hin1I GRCGYC 5 cut(s) 213, 261, 671, 710, 828
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 5 cut(s) 181, 261, 412, 710, 755
HinP1I GCGC 5 cut(s) 181, 261, 412, 710, 755
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 4 cut(s) 336, 578, 598, 778
HpaII CCGG 6 cut(s) 258, 366, 427, 463, 526, 629
HphI GGTGA 1 cut(s) 460
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 7 cut(s) 155, 178, 208, 279, 335, 669, 783
Hpy188III TCNNGA 2 cut(s) 304, 322
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 6 cut(s) 39, 70, 583, 628, 698, 766
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 3 cut(s) 213, 378, 828
HpyCH4V TGCA 3 cut(s) 310, 444, 643
HpyF10VI GCNNNNNNNGC 6 cut(s) 132, 390, 649, 718, 730, 815
HpyF3I CTNAG 4 cut(s) 12, 152, 177, 782
HpySE526I ACGT 3 cut(s) 213, 378, 828
Hsp92I GRCGYC 5 cut(s) 213, 261, 671, 710, 828
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 5 cut(s) 181, 261, 412, 710, 755
KasI GGCGCC 2 cut(s) 260, 709
Kzo9I GATC 2 cut(s) 279, 328
LmnI GCTCC 5 cut(s) 152, 164, 228, 454, 693
Lsp1109I GCAGC 2 cut(s) 371, 733
LweI GCATC 3 cut(s) 146, 297, 346
MabI ACCWGGT 1 cut(s) 495
MaeII ACGT 3 cut(s) 213, 378, 828
MaeIII GTNAC 1 cut(s) 466
MalI GATC 2 cut(s) 281, 330
MbiI CCGCTC 1 cut(s) 457
MboI GATC 2 cut(s) 279, 328
MboII GAAGA 6 cut(s) 223, 244, 274, 424, 533, 823
MflI RGATCY 1 cut(s) 279
MhlI GDGCHC 2 cut(s) 225, 698
MlsI TGGCCA 1 cut(s) 566
MluCI AATT 2 cut(s) 103, 796
MluNI TGGCCA 1 cut(s) 566
Mly113I GGCGCC 2 cut(s) 261, 710
MlyI GAGTC 2 cut(s) 587, 772
MmeI TCCRAC 1 cut(s) 692
Mox20I TGGCCA 1 cut(s) 566
MscI TGGCCA 1 cut(s) 566
MseI TTAA 3 cut(s) 89, 762, 795
Msp20I TGGCCA 1 cut(s) 566
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 6 cut(s) 258, 366, 427, 463, 526, 629
MspR9I CCNGG 6 cut(s) 144, 258, 452, 464, 497, 554
MvaI CCWGG 4 cut(s) 144, 452, 497, 554
MvnI CGCG 1 cut(s) 757
MwoI GCNNNNNNNGC 6 cut(s) 132, 390, 649, 718, 730, 815
NarI GGCGCC 2 cut(s) 261, 710
NciI CCSGG 2 cut(s) 258, 464
NdeII GATC 2 cut(s) 279, 328
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 6 cut(s) 148, 262, 370, 461, 659, 711
NmeAIII GCCGAG 2 cut(s) 529, 738
NmuCI GTSAC 1 cut(s) 466
NspV TTCGAA 1 cut(s) 702
PagI TCATGA 1 cut(s) 303
PcsI WCGNNNNNNNCGW 6 cut(s) 216, 384, 396, 405, 414, 677
PfeI GAWTC 2 cut(s) 336, 598
PflFI GACNNNGTC 3 cut(s) 212, 215, 476
PinAI ACCGGT 1 cut(s) 426
PkrI GCNGC 9 cut(s) 128, 131, 134, 386, 654, 723, 754, 759, 811
PleI GAGTC 2 cut(s) 586, 772
PluTI GGCGCC 2 cut(s) 264, 713
PpsI GAGTC 2 cut(s) 586, 772
PpuMI RGGWCCY 2 cut(s) 550, 646
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 2 cut(s) 550, 646
Psp6I CCWGG 4 cut(s) 142, 450, 495, 552
PspEI GGTNACC 1 cut(s) 466
PspGI CCWGG 4 cut(s) 142, 450, 495, 552
PspN4I GGNNCC 6 cut(s) 148, 262, 370, 461, 659, 711
PspPI GGNCC 2 cut(s) 550, 646
PspPPI RGGWCCY 2 cut(s) 550, 646
PstNI CAGNNNCTG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 279
PsyI GACNNNGTC 3 cut(s) 212, 215, 476
RsaI GTAC 1 cut(s) 484
RsaNI GTAC 1 cut(s) 483
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 3 cut(s) 89, 762, 795
SatI GCNGC 9 cut(s) 127, 130, 133, 385, 653, 722, 753, 758, 810
Sau3AI GATC 2 cut(s) 279, 328
Sau96I GGNCC 2 cut(s) 550, 646
SchI GAGTC 2 cut(s) 587, 772
ScrFI CCNGG 6 cut(s) 144, 258, 452, 464, 497, 554
SduI GDGCHC 2 cut(s) 225, 698
SexAI ACCWGGT 1 cut(s) 495
SfaNI GCATC 3 cut(s) 146, 297, 346
SfoI GGCGCC 2 cut(s) 262, 711
SfuI TTCGAA 1 cut(s) 702
SgrDI CGTCGACG 1 cut(s) 419
SinI GGWCC 2 cut(s) 550, 646
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 2 cut(s) 103, 796
SspDI GGCGCC 2 cut(s) 260, 709
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 6 cut(s) 142, 256, 450, 462, 495, 552
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 3 cut(s) 216, 381, 831
TaqI TCGA 7 cut(s) 219, 285, 420, 519, 533, 576, 702
TaqII GACCGA 1 cut(s) 341
TasI AATT 2 cut(s) 103, 796
TauI GCSGC 7 cut(s) 129, 132, 135, 655, 755, 760, 812
TfiI GAWTC 2 cut(s) 336, 598
Tru1I TTAA 3 cut(s) 89, 762, 795
Tru9I TTAA 3 cut(s) 89, 762, 795
TseFI GTSAC 1 cut(s) 466
TseI GCWGC 2 cut(s) 384, 721
Tsp45I GTSAC 1 cut(s) 466
TspDTI ATGAA 1 cut(s) 602
TspGWI ACGGA 1 cut(s) 470
Tth111I GACNNNGTC 3 cut(s) 212, 215, 476
Vha464I CTTAAG 1 cut(s) 761
VpaK11BI GGWCC 2 cut(s) 550, 646
XcmI CCANNNNNNNNNTGG 1 cut(s) 493
XmiI GTMKAC 1 cut(s) 420
ZraI GACGTC 2 cut(s) 214, 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.