pycom05g12660

LON peptidase N-terminal domain and RING finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0008497
Physical Location & Seq
Reverse (-)
16076702 .. 16077172
471 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g12660.1

Sequence Viewer

Length: 471 bp
ATGATGCACTCCCTCCTCCAGACCGATCTCCGATTCGGGGTTATCTACAATGATGCCGTCTCCGGCACCGCCGACGTTGGCTGCGTCGGCGAGGTCGTCAAGCACGAGCGCCTCGTCGATGACCGGTTCTTCCTCATCTGCAAAGGCCAGGAGCGGTTCCGGGTCACTAACCTCGTCCGTACCAAACCTTACCTGGTGGCGGAGGTCCAATGGCTCGAAGACCGGCCGTCGAACGACGGCGAGGAGGACCTGGAGGCGTTGGCCAGCGAGGTCGAGTCGTATATGAAGGATGTGATTCGGTTGTCAAATCGGTTGGGCGGGAAGCCGGAGAAGGATGTGCAGGACCTGCGACGAAACCTGTTTCCGACGCCGTTTTCGTTTTTCGTTGGGAGCACTATCGAAGGGGCACCGAGAGAGCAGCAGGCGCTGCTGGAACTGGAGGACACGGCGGCGCGGTTGAACTGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

157

Amino Acids

17.83

Weight (kDa)

4.93

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 22 - 154 8.5e-19 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 354
AccB1I GGYRCC 2 cut(s) 65, 406
AccBSI CCGCTC 1 cut(s) 154
AccII CGCG 1 cut(s) 454
AciI CCGC 6 cut(s) 69, 154, 200, 318, 449, 454
AcoI YGGCCR 2 cut(s) 224, 261
AcyI GRCGYC 1 cut(s) 368
AfaI GTAC 1 cut(s) 181
AfiI CCNNNNNNNGG 2 cut(s) 37, 199
AgeI ACCGGT 1 cut(s) 123
AgsI TTSAA 1 cut(s) 460
AhdI GACNNNNNGTC 1 cut(s) 226
AjnI CCWGG 3 cut(s) 147, 192, 249
Alw21I GWGCWC 1 cut(s) 395
Alw26I GTCTC 1 cut(s) 64
AlwNI CAGNNNCTG 2 cut(s) 346, 427
AoxI GGCC 3 cut(s) 145, 224, 261
ApeKI GCWGC 3 cut(s) 81, 418, 427
ArsI GACNNNNNNTTYG 2 cut(s) 358, 390
AsiGI ACCGGT 1 cut(s) 123
AspLEI GCGC 3 cut(s) 111, 427, 454
AspS9I GGNCC 3 cut(s) 205, 247, 343
AsuC2I CCSGG 1 cut(s) 161
AvaII GGWCC 3 cut(s) 205, 247, 343
BaeGI GKGCMC 1 cut(s) 409
BalI TGGCCA 1 cut(s) 263
BanI GGYRCC 2 cut(s) 65, 406
BauI CACGAG 1 cut(s) 104
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 395
BbvI GCAGC 3 cut(s) 68, 414, 430
BceAI ACGGC 5 cut(s) 41, 211, 253, 355, 462
BciT130I CCWGG 3 cut(s) 149, 194, 251
BcnI CCSGG 1 cut(s) 161
BcoDI GTCTC 1 cut(s) 64
BfoI RGCGCY 2 cut(s) 112, 428
BfuAI ACCTGC 1 cut(s) 354
BisI GCNGC 4 cut(s) 82, 419, 428, 450
BlsI GCNGC 4 cut(s) 83, 420, 429, 451
Bme1390I CCNGG 4 cut(s) 149, 161, 194, 251
Bme18I GGWCC 3 cut(s) 205, 247, 343
BmeRI GACNNNNNGTC 1 cut(s) 226
BmgT120I GGNCC 3 cut(s) 205, 247, 343
BmiI GGNNCC 3 cut(s) 67, 158, 408
BmrFI CCNGG 4 cut(s) 149, 161, 194, 251
BmsI GCATC 1 cut(s) 43
BpiI GAAGAC 1 cut(s) 225
BpmI CTGGAG 2 cut(s) 272, 458
BpuMI CCSGG 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 368
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 199
Bse118I RCCGGY 2 cut(s) 123, 222
Bse1I ACTGG 2 cut(s) 441, 467
BseBI CCWGG 3 cut(s) 149, 194, 251
BseGI GGATG 2 cut(s) 295, 340
BseLI CCNNNNNNNGG 2 cut(s) 37, 199
BseNI ACTGG 2 cut(s) 441, 467
BseRI GAGGAG 2 cut(s) 5, 257
BseSI GKGCMC 1 cut(s) 409
BseX3I CGGCCG 1 cut(s) 224
BseXI GCAGC 3 cut(s) 68, 414, 430
BsgI GTGCAG 1 cut(s) 359
Bsh1236I CGCG 1 cut(s) 454
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 3 cut(s) 147, 226, 263
BshNI GGYRCC 2 cut(s) 65, 406
BshTI ACCGGT 1 cut(s) 123
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 395
BsiSI CCGG 5 cut(s) 63, 124, 160, 223, 326
BslI CCNNNNNNNGG 2 cut(s) 37, 199
BsmAI GTCTC 1 cut(s) 64
BsmBI CGTCTC 1 cut(s) 64
BsnI GGCC 3 cut(s) 147, 226, 263
Bsp1286I GDGCHC 2 cut(s) 395, 409
Bsp143I GATC 1 cut(s) 25
BspACI CCGC 6 cut(s) 69, 154, 200, 318, 449, 454
BspANI GGCC 3 cut(s) 147, 226, 263
BspFNI CGCG 1 cut(s) 454
BspLI GGNNCC 3 cut(s) 67, 158, 408
BspMI ACCTGC 1 cut(s) 354
BspT107I GGYRCC 2 cut(s) 65, 406
BsrBI CCGCTC 1 cut(s) 154
BsrFI RCCGGY 2 cut(s) 123, 222
BsrI ACTGG 2 cut(s) 441, 467
BssAI RCCGGY 2 cut(s) 123, 222
BssMI GATC 1 cut(s) 25
BssNI GRCGYC 1 cut(s) 368
BssSI CACGAG 1 cut(s) 104
Bst2BI CACGAG 1 cut(s) 104
Bst2UI CCWGG 3 cut(s) 149, 194, 251
BstACI GRCGYC 1 cut(s) 368
BstAPI GCANNNNNTGC 2 cut(s) 346, 427
BstC8I GCNNGC 2 cut(s) 265, 423
BstF5I GGATG 2 cut(s) 295, 340
BstFNI CGCG 1 cut(s) 454
BstH2I RGCGCY 2 cut(s) 112, 428
BstHHI GCGC 3 cut(s) 111, 427, 454
BstKTI GATC 1 cut(s) 28
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 1 cut(s) 25
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 4 cut(s) 87, 346, 424, 427
BstNI CCWGG 3 cut(s) 149, 194, 251
BstSCI CCNGG 4 cut(s) 147, 159, 192, 249
BstSLI GKGCMC 1 cut(s) 409
BstUI CGCG 1 cut(s) 454
BstV1I GCAGC 3 cut(s) 68, 414, 430
BstV2I GAAGAC 1 cut(s) 225
BstZI CGGCCG 1 cut(s) 224
BsuRI GGCC 3 cut(s) 147, 226, 263
BtsCI GGATG 2 cut(s) 295, 340
BveI ACCTGC 1 cut(s) 354
Cac8I GCNNGC 2 cut(s) 265, 423
CaiI CAGNNNCTG 2 cut(s) 346, 427
CfoI GCGC 3 cut(s) 111, 427, 454
Cfr10I RCCGGY 2 cut(s) 123, 222
Cfr13I GGNCC 3 cut(s) 205, 247, 343
CseI GACGC 2 cut(s) 73, 376
CsiI ACCWGGT 1 cut(s) 192
Csp6I GTAC 1 cut(s) 180
CspAI ACCGGT 1 cut(s) 123
CviJI RGCY 6 cut(s) 81, 147, 214, 226, 263, 325
CviKI_1 RGCY 6 cut(s) 81, 147, 214, 226, 263, 325
CviQI GTAC 1 cut(s) 180
DpnI GATC 1 cut(s) 27
DpnII GATC 1 cut(s) 25
DriI GACNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 2 cut(s) 224, 261
EagI CGGCCG 1 cut(s) 224
Eam1105I GACNNNNNGTC 1 cut(s) 226
EciI GGCGGA 1 cut(s) 215
EclXI CGGCCG 1 cut(s) 224
Eco47I GGWCC 3 cut(s) 205, 247, 343
Eco52I CGGCCG 1 cut(s) 224
EcoO109I RGGNCCY 2 cut(s) 247, 343
EcoRII CCWGG 3 cut(s) 147, 192, 249
Esp3I CGTCTC 1 cut(s) 64
FaiI YATR 2 cut(s) 282, 284
FauI CCCGC 1 cut(s) 311
Fnu4HI GCNGC 4 cut(s) 82, 419, 428, 450
FokI GGATG 2 cut(s) 302, 347
Fsp4HI GCNGC 4 cut(s) 82, 419, 428, 450
GlaI GCGC 3 cut(s) 110, 426, 453
GluI GCNGC 4 cut(s) 82, 419, 428, 450
GsuI CTGGAG 2 cut(s) 272, 458
HaeII RGCGCY 2 cut(s) 112, 428
HaeIII GGCC 3 cut(s) 147, 226, 263
HapII CCGG 5 cut(s) 63, 124, 160, 223, 326
HgaI GACGC 2 cut(s) 73, 376
HhaI GCGC 3 cut(s) 111, 427, 454
Hin1I GRCGYC 1 cut(s) 368
Hin6I GCGC 3 cut(s) 109, 425, 452
HinP1I GCGC 3 cut(s) 109, 425, 452
HinfI GANTC 3 cut(s) 33, 275, 295
HpaII CCGG 5 cut(s) 63, 124, 160, 223, 326
Hpy188I TCNGA 2 cut(s) 32, 366
Hpy188III TCNNGA 1 cut(s) 19
Hpy99I CGWCG 7 cut(s) 77, 89, 119, 232, 239, 354, 370
HpyAV CCTTC 3 cut(s) 280, 325, 395
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 3 cut(s) 7, 141, 340
HpyF10VI GCNNNNNNNGC 4 cut(s) 87, 346, 424, 427
HpySE526I ACGT 1 cut(s) 75
Hsp92I GRCGYC 1 cut(s) 368
HspAI GCGC 3 cut(s) 109, 425, 452
Kzo9I GATC 1 cut(s) 25
LmnI GCTCC 2 cut(s) 151, 390
Lsp1109I GCAGC 3 cut(s) 68, 414, 430
LweI GCATC 1 cut(s) 43
MabI ACCWGGT 1 cut(s) 192
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 1 cut(s) 163
MalI GATC 1 cut(s) 27
MbiI CCGCTC 1 cut(s) 154
MboI GATC 1 cut(s) 25
MboII GAAGA 2 cut(s) 121, 230
MhlI GDGCHC 2 cut(s) 395, 409
MlsI TGGCCA 1 cut(s) 263
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 1 cut(s) 389
Mox20I TGGCCA 1 cut(s) 263
MscI TGGCCA 1 cut(s) 263
Msp20I TGGCCA 1 cut(s) 263
MspI CCGG 5 cut(s) 63, 124, 160, 223, 326
MspR9I CCNGG 4 cut(s) 149, 161, 194, 251
MvaI CCWGG 3 cut(s) 149, 194, 251
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 4 cut(s) 87, 346, 424, 427
NciI CCSGG 1 cut(s) 161
NdeII GATC 1 cut(s) 25
NlaIV GGNNCC 3 cut(s) 67, 158, 408
NmuCI GTSAC 1 cut(s) 163
PcsI WCGNNNNNNNCGW 5 cut(s) 81, 93, 102, 111, 374
PfeI GAWTC 2 cut(s) 33, 295
PinAI ACCGGT 1 cut(s) 123
PkrI GCNGC 4 cut(s) 83, 420, 429, 451
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PpuMI RGGWCCY 2 cut(s) 247, 343
Psp5II RGGWCCY 2 cut(s) 247, 343
Psp6I CCWGG 3 cut(s) 147, 192, 249
PspGI CCWGG 3 cut(s) 147, 192, 249
PspN4I GGNNCC 3 cut(s) 67, 158, 408
PspPI GGNCC 3 cut(s) 205, 247, 343
PspPPI RGGWCCY 2 cut(s) 247, 343
PstNI CAGNNNCTG 2 cut(s) 346, 427
RsaI GTAC 1 cut(s) 181
RsaNI GTAC 1 cut(s) 180
SatI GCNGC 4 cut(s) 82, 419, 428, 450
Sau3AI GATC 1 cut(s) 25
Sau96I GGNCC 3 cut(s) 205, 247, 343
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 4 cut(s) 149, 161, 194, 251
SduI GDGCHC 2 cut(s) 395, 409
SexAI ACCWGGT 1 cut(s) 192
SfaNI GCATC 1 cut(s) 43
SinI GGWCC 3 cut(s) 205, 247, 343
SsiI CCGC 6 cut(s) 69, 154, 200, 318, 449, 454
StyD4I CCNGG 4 cut(s) 147, 159, 192, 249
TaiI ACGT 1 cut(s) 78
TaqI TCGA 5 cut(s) 117, 216, 230, 273, 399
TaqII GACCGA 1 cut(s) 38
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 2 cut(s) 33, 295
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 3 cut(s) 81, 418, 427
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 1 cut(s) 299
TspGWI ACGGA 1 cut(s) 167
VpaK11BI GGWCC 3 cut(s) 205, 247, 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.