Rw6G036600

LON peptidase N-terminal domain and RING finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008497
Physical Location & Seq
Forward (+)
61069845 .. 61071133
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G036600.1

Sequence Viewer

Length: 858 bp
ATGGCTCTCCCTCAGCTCATTCCCTCCCCTTCTTCTTCTTCTTCCTCCCTCCACCATAAACCCTCCTTCAATCCCAATCCACTCCCCTATCCCCTATCCCCAAACCCTAATTTCTTCTCTTCCCGCCTCCGCCGCCAGCGACGCCGCTCTTCCTCCCTCACATGCTCCGCCTCCTCCTTCTCCGAGCGCCACCACACCAACTCCAAGTCCGACGACGTCGTCGAGCTCCCGCTCTTTCCCTTGCCGCTCGTCCTCTTCCCCGGCGCGATTCTGCCGCTGCAGATCTTCGAGTTCCGGTACCGGATGATGATGCACTCGCTCCTCCAGACCGATCTCCGCTTCGGCGTCATCTACAGCGACGCCGTCTCCGGCACTGCCGACGTCGGCTGCGTCGGCGAGGTCGTCAAGCACGAGCGGCTCGTCGACGACCGGTTCTTCCTGATTTGTAAAGGTCAGGAGCGTTTTAGGGTTACGGACCTGGTCCGGACCAAGCCCTACCTGGTGGCGGAGGTCAAGTGGCTGGAGGACCGGCCGTCCGGCGACGGCGAGGAGGACCTGGACACATTGGCCACCGAGGTTGAGTCCTACATGAAGGACGTGATTCGGCTCTCCAACCGCCTCGGCGGCAAGCCGGAGAAGGAGGTGCAGGACCTGAGGCGCAACCTATTTCCGACGCCGTTCTCGTTCTTCGTCGGAAGCACTTTCGAAGGCGCGCCGAGGGAGCAGCAGGCCTTGCTGGAGTTGGAGGACACGGCGGCGAGGCTGAAGAGGGAGAAGGAGACGCTGAGGAACACGCTCAATTACTTGACGGCGGCGTCCGCCGTTAAAGACGTCTTTCCGTCGTCGTCGTCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LON_substr_bdg

Protein Analysis

285

Amino Acids

32.2

Weight (kDa)

7.03

Isoelectric Point (pI)

57.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LON_substr_bdg PF02190 75 - 262 3.9e-29 ATP-dependent protease La (LON) substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 218, 814
AatII GACGTC 3 cut(s) 219, 384, 834
Acc65I GGTACC 1 cut(s) 297
AccB1I GGYRCC 1 cut(s) 297
AccBSI CCGCTC 4 cut(s) 147, 232, 247, 415
AccI GTMKAC 1 cut(s) 423
AccII CGCG 2 cut(s) 266, 713
AccIII TCCGGA 1 cut(s) 483
AcoI YGGCCR 2 cut(s) 530, 567
AcuI CTGAAG 1 cut(s) 785
AcyI GRCGYC 8 cut(s) 142, 216, 345, 360, 381, 674, 815, 831
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 1 cut(s) 505
AgeI ACCGGT 1 cut(s) 429
AgsI TTSAA 1 cut(s) 70
AhdI GACNNNNNGTC 1 cut(s) 532
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 3 cut(s) 477, 498, 555
AluBI AGCT 2 cut(s) 16, 226
AluI AGCT 2 cut(s) 16, 226
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 370, 773
AlwNI CAGNNNCTG 1 cut(s) 652
Aor13HI TCCGGA 1 cut(s) 483
AoxI GGCC 3 cut(s) 530, 567, 729
ApeKI GCWGC 3 cut(s) 277, 387, 724
AscI GGCGCGCC 1 cut(s) 711
AsiGI ACCGGT 1 cut(s) 429
Asp718I GGTACC 1 cut(s) 297
AspLEI GCGC 5 cut(s) 189, 266, 660, 713, 715
AspS9I GGNCC 6 cut(s) 475, 481, 486, 526, 553, 649
AsuC2I CCSGG 1 cut(s) 261
AsuII TTCGAA 1 cut(s) 705
AvaII GGWCC 6 cut(s) 475, 481, 486, 526, 553, 649
AxyI CCTNAGG 1 cut(s) 653
BalI TGGCCA 1 cut(s) 569
BanI GGYRCC 1 cut(s) 297
BanII GRGCYC 1 cut(s) 228
BauI CACGAG 1 cut(s) 410
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 228
BbvCI CCTCAGC 2 cut(s) 12, 785
BbvI GCAGC 3 cut(s) 264, 374, 736
BceAI ACGGC 7 cut(s) 347, 517, 559, 661, 768, 806, 825
BciT130I CCWGG 3 cut(s) 479, 500, 557
BcnI CCSGG 1 cut(s) 261
BcoDI GTCTC 2 cut(s) 370, 773
BfmI CTRYAG 2 cut(s) 278, 352
BfoI RGCGCY 1 cut(s) 190
BglI GCCNNNNNGGC 1 cut(s) 624
BglII AGATCT 1 cut(s) 282
Bme1390I CCNGG 4 cut(s) 261, 479, 500, 557
Bme18I GGWCC 6 cut(s) 475, 481, 486, 526, 553, 649
BmeRI GACNNNNNGTC 1 cut(s) 532
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 6 cut(s) 475, 481, 486, 526, 553, 649
BmiI GGNNCC 1 cut(s) 299
BmrFI CCNGG 4 cut(s) 261, 479, 500, 557
BmsI GCATC 1 cut(s) 300
BpiI GAAGAC 1 cut(s) 843
BpmI CTGGAG 3 cut(s) 308, 542, 758
Bpu10I CCTNAGC 2 cut(s) 12, 785
Bpu14I TTCGAA 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 261
BsaHI GRCGYC 8 cut(s) 142, 216, 345, 360, 381, 674, 815, 831
BsaJI CCNNGG 4 cut(s) 259, 573, 619, 716
BsaWI WCCGGW 4 cut(s) 294, 300, 429, 483
BsaXI ACNNNNNCTCC 6 cut(s) 185, 215, 350, 380, 500, 530
Bsc4I CCNNNNNNNGG 1 cut(s) 505
Bse118I RCCGGY 2 cut(s) 429, 528
Bse21I CCTNAGG 1 cut(s) 653
BseAI TCCGGA 1 cut(s) 483
BseBI CCWGG 3 cut(s) 479, 500, 557
BseDI CCNNGG 4 cut(s) 259, 573, 619, 716
BseGI GGATG 1 cut(s) 309
BseLI CCNNNNNNNGG 1 cut(s) 505
BseMII CTCAG 3 cut(s) 26, 644, 776
BsePI GCGCGC 1 cut(s) 711
BseRI GAGGAG 3 cut(s) 163, 311, 563
BseX3I CGGCCG 1 cut(s) 530
BseXI GCAGC 3 cut(s) 264, 374, 736
BsgI GTGCAG 1 cut(s) 665
Bsh1236I CGCG 2 cut(s) 266, 713
Bsh1285I CGRYCG 2 cut(s) 430, 533
BshFI GGCC 3 cut(s) 532, 569, 731
BshNI GGYRCC 1 cut(s) 297
BshTI ACCGGT 1 cut(s) 429
BsiEI CGRYCG 2 cut(s) 430, 533
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 9 cut(s) 261, 295, 301, 369, 430, 484, 529, 537, 632
BslI CCNNNNNNNGG 1 cut(s) 505
BsmAI GTCTC 2 cut(s) 370, 773
BsmBI CGTCTC 2 cut(s) 370, 773
BsnI GGCC 3 cut(s) 532, 569, 731
Bsp119I TTCGAA 1 cut(s) 705
Bsp1286I GDGCHC 1 cut(s) 228
Bsp13I TCCGGA 1 cut(s) 483
Bsp143I GATC 2 cut(s) 282, 331
BspANI GGCC 3 cut(s) 532, 569, 731
BspCNI CTCAG 3 cut(s) 25, 645, 777
BspEI TCCGGA 1 cut(s) 483
BspFNI CGCG 2 cut(s) 266, 713
BspLI GGNNCC 1 cut(s) 299
BspMAI CTGCAG 1 cut(s) 282
BspQI GCTCTTC 1 cut(s) 154
BspT104I TTCGAA 1 cut(s) 705
BspT107I GGYRCC 1 cut(s) 297
BsrBI CCGCTC 4 cut(s) 147, 232, 247, 415
BsrFI RCCGGY 2 cut(s) 429, 528
BssAI RCCGGY 2 cut(s) 429, 528
BssECI CCNNGG 4 cut(s) 259, 573, 619, 716
BssHII GCGCGC 1 cut(s) 711
BssMI GATC 2 cut(s) 282, 331
BssNI GRCGYC 8 cut(s) 142, 216, 345, 360, 381, 674, 815, 831
BssSI CACGAG 1 cut(s) 410
Bst2BI CACGAG 1 cut(s) 410
Bst2UI CCWGG 3 cut(s) 479, 500, 557
Bst6I CTCTTC 4 cut(s) 124, 154, 260, 761
BstACI GRCGYC 8 cut(s) 142, 216, 345, 360, 381, 674, 815, 831
BstAPI GCANNNNNTGC 1 cut(s) 733
BstBI TTCGAA 1 cut(s) 705
BstC8I GCNNGC 4 cut(s) 137, 629, 713, 729
BstDEI CTNAG 3 cut(s) 12, 653, 785
BstF5I GGATG 1 cut(s) 309
BstFNI CGCG 2 cut(s) 266, 713
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 5 cut(s) 189, 266, 660, 713, 715
BstKTI GATC 2 cut(s) 285, 334
BstMAI GTCTC 2 cut(s) 370, 773
BstMBI GATC 2 cut(s) 282, 331
BstMCI CGRYCG 2 cut(s) 430, 533
BstMWI GCNNNNNNNGC 8 cut(s) 132, 141, 393, 415, 624, 721, 733, 818
BstNI CCWGG 3 cut(s) 479, 500, 557
BstNSI RCATGY 1 cut(s) 165
BstSCI CCNGG 4 cut(s) 259, 477, 498, 555
BstSFI CTRYAG 2 cut(s) 278, 352
BstUI CGCG 2 cut(s) 266, 713
BstV1I GCAGC 3 cut(s) 264, 374, 736
BstV2I GAAGAC 1 cut(s) 843
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BstZI CGGCCG 1 cut(s) 530
Bsu36I CCTNAGG 1 cut(s) 653
BsuRI GGCC 3 cut(s) 532, 569, 731
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 1 cut(s) 309
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 372
Cac8I GCNNGC 4 cut(s) 137, 629, 713, 729
CaiI CAGNNNCTG 1 cut(s) 652
CfoI GCGC 5 cut(s) 189, 266, 660, 713, 715
Cfr10I RCCGGY 2 cut(s) 429, 528
Cfr13I GGNCC 6 cut(s) 475, 481, 486, 526, 553, 649
CseI GACGC 7 cut(s) 150, 334, 368, 379, 682, 790, 804
CsiI ACCWGGT 2 cut(s) 477, 498
Csp6I GTAC 1 cut(s) 298
CspAI ACCGGT 1 cut(s) 429
CspCI CAANNNNNGTGG 2 cut(s) 559, 594
CviAII CATG 2 cut(s) 162, 589
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 3 cut(s) 12, 653, 785
DpnI GATC 2 cut(s) 284, 333
DpnII GATC 2 cut(s) 282, 331
DrdI GACNNNNNNGTC 2 cut(s) 218, 814
DriI GACNNNNNGTC 1 cut(s) 532
DseDI GACNNNNNNGTC 2 cut(s) 218, 814
EaeI YGGCCR 2 cut(s) 530, 567
EagI CGGCCG 1 cut(s) 530
Eam1104I CTCTTC 4 cut(s) 124, 154, 260, 761
Eam1105I GACNNNNNGTC 1 cut(s) 532
EarI CTCTTC 4 cut(s) 124, 154, 260, 761
EciI GGCGGA 4 cut(s) 119, 157, 521, 808
Ecl136II GAGCTC 1 cut(s) 226
EclXI CGGCCG 1 cut(s) 530
Eco147I AGGCCT 1 cut(s) 731
Eco24I GRGCYC 1 cut(s) 228
Eco47I GGWCC 6 cut(s) 475, 481, 486, 526, 553, 649
Eco52I CGGCCG 1 cut(s) 530
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 785
Eco81I CCTNAGG 1 cut(s) 653
EcoICRI GAGCTC 1 cut(s) 226
EcoO109I RGGNCCY 2 cut(s) 553, 649
EcoRII CCWGG 3 cut(s) 477, 498, 555
EcoT38I GRGCYC 1 cut(s) 228
Esp3I CGTCTC 2 cut(s) 370, 773
FaeI CATG 2 cut(s) 165, 592
FaiI YATR 3 cut(s) 57, 163, 590
FatI CATG 2 cut(s) 161, 588
FauI CCCGC 2 cut(s) 131, 237
FblI GTMKAC 1 cut(s) 423
FokI GGATG 1 cut(s) 316
FriOI GRGCYC 1 cut(s) 228
GlaI GCGC 5 cut(s) 188, 265, 659, 712, 714
GsuI CTGGAG 3 cut(s) 308, 542, 758
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 3 cut(s) 532, 569, 731
HapII CCGG 9 cut(s) 261, 295, 301, 369, 430, 484, 529, 537, 632
HgaI GACGC 7 cut(s) 150, 334, 368, 379, 682, 790, 804
HhaI GCGC 5 cut(s) 189, 266, 660, 713, 715
Hin1I GRCGYC 8 cut(s) 142, 216, 345, 360, 381, 674, 815, 831
Hin1II CATG 2 cut(s) 165, 592
Hin6I GCGC 5 cut(s) 187, 264, 658, 711, 713
HinP1I GCGC 5 cut(s) 187, 264, 658, 711, 713
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 3 cut(s) 268, 581, 601
HpaII CCGG 9 cut(s) 261, 295, 301, 369, 430, 484, 529, 537, 632
Hpy166II GTNNAC 1 cut(s) 424
Hpy188I TCNGA 4 cut(s) 184, 211, 672, 695
Hpy188III TCNNGA 5 cut(s) 325, 439, 455, 484, 855
Hpy8I GTNNAC 1 cut(s) 424
HpyAV CCTTC 7 cut(s) 39, 76, 187, 586, 631, 701, 769
HpyCH4IV ACGT 4 cut(s) 216, 381, 597, 831
HpyCH4V TGCA 3 cut(s) 280, 313, 646
HpyF10VI GCNNNNNNNGC 8 cut(s) 132, 141, 393, 415, 624, 721, 733, 818
HpyF3I CTNAG 3 cut(s) 12, 653, 785
HpySE526I ACGT 4 cut(s) 216, 381, 597, 831
Hsp92I GRCGYC 8 cut(s) 142, 216, 345, 360, 381, 674, 815, 831
Hsp92II CATG 2 cut(s) 165, 592
HspAI GCGC 5 cut(s) 187, 264, 658, 711, 713
Kpn2I TCCGGA 1 cut(s) 483
KpnI GGTACC 1 cut(s) 301
Kzo9I GATC 2 cut(s) 282, 331
LguI GCTCTTC 1 cut(s) 154
LmnI GCTCC 5 cut(s) 170, 231, 324, 457, 721
Lsp1109I GCAGC 3 cut(s) 264, 374, 736
LweI GCATC 1 cut(s) 300
MabI ACCWGGT 2 cut(s) 477, 498
MaeII ACGT 4 cut(s) 216, 381, 597, 831
MaeIII GTNAC 1 cut(s) 469
MalI GATC 2 cut(s) 284, 333
MbiI CCGCTC 4 cut(s) 147, 232, 247, 415
MboI GATC 2 cut(s) 282, 331
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 228
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 2 cut(s) 109, 799
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 1 cut(s) 590
MmeI TCCRAC 5 cut(s) 234, 636, 673, 695, 723
Mox20I TGGCCA 1 cut(s) 569
MroI TCCGGA 1 cut(s) 483
MscI TGGCCA 1 cut(s) 569
MseI TTAA 1 cut(s) 825
Msp20I TGGCCA 1 cut(s) 569
MspA1I CMGCKG 1 cut(s) 277
MspI CCGG 9 cut(s) 261, 295, 301, 369, 430, 484, 529, 537, 632
MspR9I CCNGG 4 cut(s) 261, 479, 500, 557
MvaI CCWGG 3 cut(s) 479, 500, 557
MvnI CGCG 2 cut(s) 266, 713
MwoI GCNNNNNNNGC 8 cut(s) 132, 141, 393, 415, 624, 721, 733, 818
NciI CCSGG 1 cut(s) 261
NdeII GATC 2 cut(s) 282, 331
NlaIII CATG 2 cut(s) 165, 592
NlaIV GGNNCC 1 cut(s) 299
NmeAIII GCCGAG 2 cut(s) 600, 741
NspI RCATGY 1 cut(s) 165
NspV TTCGAA 1 cut(s) 705
PalAI GGCGCGCC 1 cut(s) 711
PauI GCGCGC 1 cut(s) 711
PceI AGGCCT 1 cut(s) 731
PciSI GCTCTTC 1 cut(s) 154
PcsI WCGNNNNNNNCGW 6 cut(s) 219, 387, 399, 408, 417, 680
PfeI GAWTC 2 cut(s) 268, 601
PflFI GACNNNGTC 4 cut(s) 215, 218, 362, 479
PinAI ACCGGT 1 cut(s) 429
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
PpuMI RGGWCCY 2 cut(s) 553, 649
Psp124BI GAGCTC 1 cut(s) 228
Psp5II RGGWCCY 2 cut(s) 553, 649
Psp6I CCWGG 3 cut(s) 477, 498, 555
PspGI CCWGG 3 cut(s) 477, 498, 555
PspN4I GGNNCC 1 cut(s) 299
PspPI GGNCC 6 cut(s) 475, 481, 486, 526, 553, 649
PspPPI RGGWCCY 2 cut(s) 553, 649
PstI CTGCAG 1 cut(s) 282
PstNI CAGNNNCTG 1 cut(s) 652
PsuI RGATCY 1 cut(s) 282
PsyI GACNNNGTC 4 cut(s) 215, 218, 362, 479
PteI GCGCGC 1 cut(s) 711
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
SacI GAGCTC 1 cut(s) 228
SalI GTCGAC 1 cut(s) 422
SapI GCTCTTC 1 cut(s) 154
SaqAI TTAA 1 cut(s) 825
Sau3AI GATC 2 cut(s) 282, 331
Sau96I GGNCC 6 cut(s) 475, 481, 486, 526, 553, 649
SchI GAGTC 1 cut(s) 590
ScrFI CCNGG 4 cut(s) 261, 479, 500, 557
SduI GDGCHC 1 cut(s) 228
SexAI ACCWGGT 2 cut(s) 477, 498
SfaNI GCATC 1 cut(s) 300
SfcI CTRYAG 2 cut(s) 278, 352
SfuI TTCGAA 1 cut(s) 705
SgrDI CGTCGACG 1 cut(s) 422
SgsI GGCGCGCC 1 cut(s) 711
SinI GGWCC 6 cut(s) 475, 481, 486, 526, 553, 649
Sse9I AATT 2 cut(s) 109, 799
SseBI AGGCCT 1 cut(s) 731
SstI GAGCTC 1 cut(s) 228
StuI AGGCCT 1 cut(s) 731
StyD4I CCNGG 4 cut(s) 259, 477, 498, 555
TaiI ACGT 4 cut(s) 219, 384, 600, 834
TaqI TCGA 4 cut(s) 222, 288, 423, 705
TaqII GACCGA 1 cut(s) 344
TasI AATT 2 cut(s) 109, 799
TauI GCSGC 8 cut(s) 135, 147, 247, 277, 418, 627, 758, 815
TfiI GAWTC 2 cut(s) 268, 601
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 1 cut(s) 379
TseI GCWGC 3 cut(s) 277, 387, 724
TspDTI ATGAA 1 cut(s) 605
TspGWI ACGGA 2 cut(s) 488, 828
TspRI CASTG 1 cut(s) 379
Tth111I GACNNNGTC 4 cut(s) 215, 218, 362, 479
VpaK11BI GGWCC 6 cut(s) 475, 481, 486, 526, 553, 649
XceI RCATGY 1 cut(s) 165
XcmI CCANNNNNNNNNTGG 1 cut(s) 496
XmiI GTMKAC 1 cut(s) 423
ZraI GACGTC 3 cut(s) 217, 382, 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.