AT2G03800

D-aminoacyl-tRNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008523
Physical Location & Seq
Forward (+)
1156593 .. 1159138
2546 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G03800.1

Sequence Viewer

Length: 954 bp
ATGGTAACACTAATCGTGGCCACCACCGCCGATCCAGCGTCGATCAACCCTGCGGCGGCGCTTCTCGCCATGCCCGGTTGGACCGCCGGACCTATCTTACCGCCGGACATAAAGAGTTTTAGTAACAAGCAAACGAGAGTGATTCAACACGATAGATCTATAGTAAAAGAAGACGATCTCGATTTACGTTGGGAAGAAGCTACCGGTGAAGTTGTTGATGAAGTCATCTTCCTCAGCCGTCACACCGCCGTCTCAAATCGTCCTGCTTTAACTGTTCATCCGATTGGAGTGCTTCATCTAAAGGATGGAGAATCACCGCCGCAAGGAGGAAAGCCTGGATGGGCGGCGTTGCCAAGCACTAGAATTGGTCCATGGTTTCGTCTTTTGAAGAAAATGGCTGAAGCTCATGGTTTAGTTCCTGAGTTTGAGATAACATTGGAAGCTACTCATCATGGACCAATAACTAACAAGCCAACGATGTTTTTGGAGATTGGTAGTACCGAGGAATACTGGAAGAGGCAAGACGCAGCTCAAGTCATGGCTCTTTTAATGTGGGAAGGTCTTGGGCTAGGAGGCAGTGAAGAAGTAGGGAAGTGGAAAAGTGAAACCGGAAAGAGGAAAGTTCTTTTAGGCATTGGAGGTGGACATTATGCTCCTCGTCACATGGATATAGCTTTGAAAGATGATATTTGGGTAGGCCATTTGCTTTCTGGATACTCATTACCGATGGAAGATCCAACTCAAACCAAAACAACGCCTGGAGAGAATTACATTGGTGGAAATTGGAGACAATCAATTAAGGCAGCATTTGAAGCTACTAAAGCATCATTCCCCGGAGGCGAGATCTTGGCTCATCTTGACCACAAGAGCTTCAAAGGATGGCAAAAGAAGGCCATTACAGAGTTTTTAGCAGAAGAGAGCATCAATGTTGGGAAGCCAAACGATTTCACATGA

Protein Analysis

317

Amino Acids

34.73

Weight (kDa)

6.07

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 70 - 310 1.5e-57 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 9 cut(s) 27, 53, 56, 84, 101, 246, 317, 320, 344
AclWI GGATC 2 cut(s) 26, 728
AcoI YGGCCR 1 cut(s) 18
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 1 cut(s) 499
AfiI CCNNNNNNNGG 4 cut(s) 55, 323, 326, 615
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 5 cut(s) 146, 388, 679, 812, 874
AjnI CCWGG 2 cut(s) 334, 757
AluBI AGCT 7 cut(s) 200, 404, 443, 530, 674, 815, 870
AluI AGCT 7 cut(s) 200, 404, 443, 530, 674, 815, 870
Alw26I GTCTC 2 cut(s) 256, 781
AlwI GGATC 2 cut(s) 26, 728
AoxI GGCC 3 cut(s) 18, 697, 891
ApeKI GCWGC 2 cut(s) 527, 803
AsiGI ACCGGT 1 cut(s) 203
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 4 cut(s) 81, 89, 368, 455
AsuC2I CCSGG 2 cut(s) 75, 834
AsuHPI GGTGA 2 cut(s) 218, 306
AvaII GGWCC 4 cut(s) 81, 89, 368, 455
BalI TGGCCA 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 177
BbvCI CCTCAGC 1 cut(s) 233
BbvI GCAGC 2 cut(s) 539, 815
BccI CCATC 4 cut(s) 299, 333, 721, 873
BceAI ACGGC 2 cut(s) 222, 233
BcgI CGANNNNNNTGC 2 cut(s) 271, 305
BciT130I CCWGG 2 cut(s) 336, 759
BciVI GTATCC 1 cut(s) 707
BcnI CCSGG 2 cut(s) 75, 834
BcoDI GTCTC 2 cut(s) 256, 781
BfaI CTAG 2 cut(s) 360, 569
BfmI CTRYAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 62
BfuI GTATCC 1 cut(s) 707
BglII AGATCT 2 cut(s) 155, 843
BisI GCNGC 6 cut(s) 54, 57, 320, 345, 528, 804
BlsI GCNGC 6 cut(s) 55, 58, 321, 346, 529, 805
Bme1390I CCNGG 4 cut(s) 75, 336, 759, 834
Bme18I GGWCC 4 cut(s) 81, 89, 368, 455
BmgT120I GGNCC 4 cut(s) 81, 89, 368, 455
BmrFI CCNGG 4 cut(s) 75, 336, 759, 834
BmsI GCATC 2 cut(s) 833, 930
BpiI GAAGAC 1 cut(s) 177
BpmI CTGGAG 1 cut(s) 780
Bpu10I CCTNAGC 1 cut(s) 233
BpuEI CTTGAG 1 cut(s) 516
BpuMI CCSGG 2 cut(s) 75, 834
BsaJI CCNNGG 3 cut(s) 371, 501, 832
BsaWI WCCGGW 2 cut(s) 203, 608
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 323, 326, 615
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 515
BseBI CCWGG 2 cut(s) 336, 759
BseDI CCNNGG 3 cut(s) 371, 501, 832
BseGI GGATG 4 cut(s) 277, 310, 344, 884
BseLI CCNNNNNNNGG 4 cut(s) 55, 323, 326, 615
BseMII CTCAG 2 cut(s) 247, 411
BseNI ACTGG 1 cut(s) 515
BseRI GAGGAG 1 cut(s) 645
BseXI GCAGC 2 cut(s) 539, 815
BshFI GGCC 3 cut(s) 20, 699, 893
BshTI ACCGGT 1 cut(s) 203
BsiSI CCGG 6 cut(s) 75, 87, 104, 204, 609, 834
BslI CCNNNNNNNGG 4 cut(s) 55, 323, 326, 615
BsmAI GTCTC 2 cut(s) 256, 781
BsmBI CGTCTC 1 cut(s) 256
BsnI GGCC 3 cut(s) 20, 699, 893
Bsp143I GATC 6 cut(s) 31, 42, 155, 175, 733, 843
Bsp19I CCATGG 1 cut(s) 371
BspACI CCGC 9 cut(s) 27, 53, 56, 84, 101, 246, 317, 320, 344
BspANI GGCC 3 cut(s) 20, 699, 893
BspCNI CTCAG 2 cut(s) 246, 412
BspPI GGATC 2 cut(s) 26, 728
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 1 cut(s) 515
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 3 cut(s) 371, 501, 832
BssMI GATC 6 cut(s) 31, 42, 155, 175, 733, 843
BssT1I CCWWGG 1 cut(s) 371
Bst2UI CCWGG 2 cut(s) 336, 759
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 2 cut(s) 509, 909
BstDEI CTNAG 2 cut(s) 233, 420
BstDSI CCRYGG 1 cut(s) 371
BstF5I GGATG 4 cut(s) 277, 310, 344, 884
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 6 cut(s) 34, 45, 158, 178, 736, 846
BstMAI GTCTC 2 cut(s) 256, 781
BstMBI GATC 6 cut(s) 31, 42, 155, 175, 733, 843
BstMWI GCNNNNNNNGC 5 cut(s) 26, 35, 65, 812, 821
BstNI CCWGG 2 cut(s) 336, 759
BstSCI CCNGG 4 cut(s) 73, 334, 757, 832
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 539, 815
BstV2I GAAGAC 1 cut(s) 177
BstX2I RGATCY 3 cut(s) 155, 733, 843
BstYI RGATCY 3 cut(s) 155, 733, 843
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 3 cut(s) 20, 699, 893
BtgI CCRYGG 1 cut(s) 371
BtsCI GGATG 4 cut(s) 277, 310, 344, 884
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 1 cut(s) 583
CfoI GCGC 1 cut(s) 61
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 4 cut(s) 81, 89, 368, 455
CseI GACGC 2 cut(s) 27, 533
Csp6I GTAC 1 cut(s) 498
CspAI ACCGGT 1 cut(s) 203
CviAII CATG 7 cut(s) 70, 372, 407, 452, 538, 664, 951
CviQI GTAC 1 cut(s) 498
DdeI CTNAG 2 cut(s) 233, 420
DpnI GATC 6 cut(s) 33, 44, 157, 177, 735, 845
DpnII GATC 6 cut(s) 31, 42, 155, 175, 733, 843
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 2 cut(s) 509, 909
EarI CTCTTC 2 cut(s) 509, 909
Eco130I CCWWGG 1 cut(s) 371
Eco47I GGWCC 4 cut(s) 81, 89, 368, 455
Eco57I CTGAAG 1 cut(s) 420
EcoRII CCWGG 2 cut(s) 334, 757
EcoT14I CCWWGG 1 cut(s) 371
ErhI CCWWGG 1 cut(s) 371
Esp3I CGTCTC 1 cut(s) 256
FaeI CATG 7 cut(s) 73, 375, 410, 455, 541, 667, 954
FatI CATG 7 cut(s) 69, 371, 406, 451, 537, 663, 950
Fnu4HI GCNGC 6 cut(s) 54, 57, 320, 345, 528, 804
FokI GGATG 4 cut(s) 264, 317, 351, 891
Fsp4HI GCNGC 6 cut(s) 54, 57, 320, 345, 528, 804
FspBI CTAG 2 cut(s) 360, 569
GlaI GCGC 1 cut(s) 60
GluI GCNGC 6 cut(s) 54, 57, 320, 345, 528, 804
GsuI CTGGAG 1 cut(s) 780
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 3 cut(s) 20, 699, 893
HapII CCGG 6 cut(s) 75, 87, 104, 204, 609, 834
HgaI GACGC 2 cut(s) 27, 533
HhaI GCGC 1 cut(s) 61
Hin1II CATG 7 cut(s) 73, 375, 410, 455, 541, 667, 954
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HinfI GANTC 2 cut(s) 142, 311
HpaII CCGG 6 cut(s) 75, 87, 104, 204, 609, 834
HphI GGTGA 2 cut(s) 218, 306
Hpy166II GTNNAC 1 cut(s) 644
Hpy188I TCNGA 1 cut(s) 282
Hpy188III TCNNGA 4 cut(s) 179, 419, 711, 857
Hpy8I GTNNAC 1 cut(s) 644
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 2 cut(s) 551, 883
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 1 cut(s) 187
HpyF10VI GCNNNNNNNGC 5 cut(s) 26, 35, 65, 812, 821
HpyF3I CTNAG 2 cut(s) 233, 420
HpySE526I ACGT 1 cut(s) 187
Hsp92II CATG 7 cut(s) 73, 375, 410, 455, 541, 667, 954
HspAI GCGC 1 cut(s) 59
Kzo9I GATC 6 cut(s) 31, 42, 155, 175, 733, 843
LmnI GCTCC 1 cut(s) 658
Lsp1109I GCAGC 2 cut(s) 539, 815
LweI GCATC 2 cut(s) 833, 930
MaeI CTAG 2 cut(s) 360, 569
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 4 cut(s) 4, 122, 239, 659
MalI GATC 6 cut(s) 33, 44, 157, 177, 735, 845
MboI GATC 6 cut(s) 31, 42, 155, 175, 733, 843
MboII GAAGA 8 cut(s) 182, 206, 220, 400, 526, 593, 743, 926
MflI RGATCY 3 cut(s) 155, 733, 843
MlsI TGGCCA 1 cut(s) 20
MluCI AATT 4 cut(s) 363, 766, 781, 795
MluNI TGGCCA 1 cut(s) 20
MmeI TCCRAC 2 cut(s) 59, 761
MnlI CCTC 9 cut(s) 242, 320, 496, 510, 566, 609, 632, 666, 830
Mox20I TGGCCA 1 cut(s) 20
MscI TGGCCA 1 cut(s) 20
MseI TTAA 3 cut(s) 269, 548, 798
Msp20I TGGCCA 1 cut(s) 20
MspI CCGG 6 cut(s) 75, 87, 104, 204, 609, 834
MspR9I CCNGG 4 cut(s) 75, 336, 759, 834
MvaI CCWGG 2 cut(s) 336, 759
MwoI GCNNNNNNNGC 5 cut(s) 26, 35, 65, 812, 821
NciI CCSGG 2 cut(s) 75, 834
NcoI CCATGG 1 cut(s) 371
NdeII GATC 6 cut(s) 31, 42, 155, 175, 733, 843
NlaIII CATG 7 cut(s) 73, 375, 410, 455, 541, 667, 954
NmuCI GTSAC 2 cut(s) 239, 659
PfeI GAWTC 2 cut(s) 142, 311
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 6 cut(s) 55, 58, 321, 346, 529, 805
Psp6I CCWGG 2 cut(s) 334, 757
PspGI CCWGG 2 cut(s) 334, 757
PspPI GGNCC 4 cut(s) 81, 89, 368, 455
PsuI RGATCY 3 cut(s) 155, 733, 843
RsaI GTAC 1 cut(s) 499
RsaNI GTAC 1 cut(s) 498
SaqAI TTAA 3 cut(s) 269, 548, 798
SatI GCNGC 6 cut(s) 54, 57, 320, 345, 528, 804
Sau3AI GATC 6 cut(s) 31, 42, 155, 175, 733, 843
Sau96I GGNCC 4 cut(s) 81, 89, 368, 455
ScrFI CCNGG 4 cut(s) 75, 336, 759, 834
SfaNI GCATC 2 cut(s) 833, 930
SfcI CTRYAG 1 cut(s) 159
SinI GGWCC 4 cut(s) 81, 89, 368, 455
SmlI CTYRAG 1 cut(s) 531
SmoI CTYRAG 1 cut(s) 531
Sse9I AATT 4 cut(s) 363, 766, 781, 795
SsiI CCGC 9 cut(s) 27, 53, 56, 84, 101, 246, 317, 320, 344
SspMI CTAG 2 cut(s) 360, 569
StyD4I CCNGG 4 cut(s) 73, 334, 757, 832
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 1 cut(s) 190
TaqI TCGA 2 cut(s) 41, 180
TasI AATT 4 cut(s) 363, 766, 781, 795
TauI GCSGC 4 cut(s) 56, 59, 322, 347
TfiI GAWTC 2 cut(s) 142, 311
Tru1I TTAA 3 cut(s) 269, 548, 798
Tru9I TTAA 3 cut(s) 269, 548, 798
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 2 cut(s) 239, 659
TseI GCWGC 2 cut(s) 527, 803
Tsp45I GTSAC 2 cut(s) 239, 659
TspDTI ATGAA 3 cut(s) 234, 266, 284
TspRI CASTG 1 cut(s) 583
VpaK11BI GGWCC 4 cut(s) 81, 89, 368, 455
XcmI CCANNNNNNNNNTGG 1 cut(s) 707
XspI CTAG 2 cut(s) 360, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.