MD16G1055500.v1.1

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008523
Physical Location & Seq
Reverse (-)
3914585 .. 3922863
8279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1055500.v1.1.491

Sequence Viewer

Length: 888 bp
ATGCCTGGGTGGGACCCTTCCCCTTCCTGTTTACAGGACATGGAGGGGTTTGCTAATGGAGGAGGAGGAGTGAGGGTTTTGCTGCACAACAGGGGAATTGTGGAAGAAGACGACTTGGACAGGCGGTGGGAGGAGGCCACCGGGGAGCGAGTCAACGAGGTGATATTCCTGAGCAAACACACCGCCGTCTCCAACCGGCCGGCACTGACGGTACACCCGATCGGAATCCCTCATCTGCGTGAAGGCGAGGCTGGGCCGCAAGACGGGAAGCCGTGTTGGGCTGCGCCGCCGAGCTCCTGCATTGGACCCTGGCTCAGGCACCTGAAGAAGCTTGCCCAATCCCACAACTTGGTTCCTGAGTTCGAGATTACATTGGAGGGTACGCATCATGGACCGGTAACGAATATACGCCCACTATGCGGTACAGATGAGTACTGGAAGAGGCAGGATGCAGTACAAGTTATTGCTCTATTGGTTTGGGAAGGACTTGGGCTTGGAGGAGGAGCTGCTGTTGGAAACTGGAACGGGTATAGTCCTGTAAGTTCTTCTTGGATTAAGTGGTGGACATTATGCTCCTCGGCACATGGATATAGTCCTGGGTGGAAAGACCGGGTGTGGGTAGGCCATCTACTTTCGGGTTATTCCTTGCCGATGGAAGATCCAAATCAGTCCAAAGCAGGAACAAATGCAAAAGATACGAAAGACTTTGGTGGAACTTGGAAACACGCAATCAAAGCAGCATTTGAGGGTACCCAGTGGGCTTTCCCTGGTGGGGAAGTTATAGCTCATCTTGATCAAACGAGTTTTAAAAGCTGGGAAAAGAACGCTATAACGGCTTTCTTAGGCGAGCAGAACATCAAAATTGGCAAACCCAACGATTTCAACTAG

Protein Analysis

296

Amino Acids

32.37

Weight (kDa)

6.05

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 48 - 164 1.2e-21 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 749
AccB1I GGYRCC 2 cut(s) 318, 749
AccB7I CCANNNNNTGG 1 cut(s) 349
AciI CCGC 5 cut(s) 124, 183, 257, 287, 420
AclWI GGATC 1 cut(s) 653
AcoI YGGCCR 1 cut(s) 197
AcuI CTGAAG 1 cut(s) 344
AfaI GTAC 6 cut(s) 213, 382, 424, 434, 456, 751
AfiI CCNNNNNNNGG 6 cut(s) 263, 315, 349, 419, 616, 772
AgeI ACCGGT 1 cut(s) 394
AgsI TTSAA 1 cut(s) 883
AjnI CCWGG 4 cut(s) 4, 308, 595, 766
AluBI AGCT 5 cut(s) 294, 331, 506, 785, 813
AluI AGCT 5 cut(s) 294, 331, 506, 785, 813
Alw21I GWGCWC 1 cut(s) 296
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 1 cut(s) 653
AoxI GGCC 4 cut(s) 135, 197, 254, 622
ApeKI GCWGC 4 cut(s) 82, 281, 506, 737
AsiGI ACCGGT 1 cut(s) 394
Asp718I GGTACC 1 cut(s) 749
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 4 cut(s) 13, 254, 305, 392
AsuC2I CCSGG 2 cut(s) 142, 611
AsuHPI GGTGA 1 cut(s) 172
AvaII GGWCC 3 cut(s) 13, 305, 392
BanI GGYRCC 2 cut(s) 318, 749
BanII GRGCYC 1 cut(s) 296
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 296
BbvI GCAGC 4 cut(s) 69, 268, 493, 749
BccI CCATC 2 cut(s) 633, 646
BceAI ACGGC 3 cut(s) 170, 256, 849
BciT130I CCWGG 4 cut(s) 6, 310, 597, 768
BclI TGATCA 1 cut(s) 793
BcnI CCSGG 2 cut(s) 142, 611
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 1 cut(s) 886
BisI GCNGC 6 cut(s) 83, 257, 282, 287, 507, 738
BlsI GCNGC 6 cut(s) 84, 258, 283, 288, 508, 739
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 6 cut(s) 6, 142, 310, 597, 611, 768
Bme18I GGWCC 3 cut(s) 13, 305, 392
BmgT120I GGNCC 4 cut(s) 13, 254, 305, 392
BmiI GGNNCC 6 cut(s) 14, 15, 307, 320, 354, 751
BmrFI CCNGG 6 cut(s) 6, 142, 310, 597, 611, 768
BmrI ACTGGG 1 cut(s) 748
BmsI GCATC 2 cut(s) 394, 439
BmuI ACTGGG 1 cut(s) 748
BpiI GAAGAC 1 cut(s) 114
Bpu10I CCTNAGC 2 cut(s) 170, 314
BpuMI CCSGG 2 cut(s) 142, 611
BsaBI GATNNNNATC 2 cut(s) 224, 663
BsaJI CCNNGG 6 cut(s) 5, 141, 308, 576, 596, 766
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 6 cut(s) 54, 60, 84, 90, 495, 525
Bsc4I CCNNNNNNNGG 6 cut(s) 263, 315, 349, 419, 616, 772
Bse118I RCCGGY 3 cut(s) 195, 199, 394
Bse1I ACTGG 3 cut(s) 440, 524, 754
Bse8I GATNNNNATC 2 cut(s) 224, 663
BseBI CCWGG 4 cut(s) 6, 310, 597, 768
BseDI CCNNGG 6 cut(s) 5, 141, 308, 576, 596, 766
BseGI GGATG 1 cut(s) 454
BseJI GATNNNNATC 2 cut(s) 224, 663
BseLI CCNNNNNNNGG 6 cut(s) 263, 315, 349, 419, 616, 772
BseMII CTCAG 3 cut(s) 161, 328, 348
BseNI ACTGG 3 cut(s) 440, 524, 754
BseRI GAGGAG 7 cut(s) 75, 78, 81, 146, 513, 516, 565
BseX3I CGGCCG 1 cut(s) 197
BseXI GCAGC 4 cut(s) 69, 268, 493, 749
BseYI CCCAGC 2 cut(s) 251, 813
BsgI GTGCAG 1 cut(s) 68
Bsh1285I CGRYCG 2 cut(s) 200, 222
BshFI GGCC 4 cut(s) 137, 199, 256, 624
BshNI GGYRCC 2 cut(s) 318, 749
BshTI ACCGGT 1 cut(s) 394
BsiEI CGRYCG 2 cut(s) 200, 222
BsiHKAI GWGCWC 1 cut(s) 296
BsiSI CCGG 5 cut(s) 141, 196, 200, 395, 610
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 6 cut(s) 263, 315, 349, 419, 616, 772
BsmAI GTCTC 1 cut(s) 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 4 cut(s) 137, 199, 256, 624
Bsp1286I GDGCHC 1 cut(s) 296
Bsp143I GATC 3 cut(s) 219, 658, 793
BspACI CCGC 5 cut(s) 124, 183, 257, 287, 420
BspANI GGCC 4 cut(s) 137, 199, 256, 624
BspCNI CTCAG 3 cut(s) 162, 327, 349
BspLI GGNNCC 6 cut(s) 14, 15, 307, 320, 354, 751
BspPI GGATC 1 cut(s) 653
BspT107I GGYRCC 2 cut(s) 318, 749
BsrFI RCCGGY 3 cut(s) 195, 199, 394
BsrI ACTGG 3 cut(s) 440, 524, 754
BssAI RCCGGY 3 cut(s) 195, 199, 394
BssECI CCNNGG 6 cut(s) 5, 141, 308, 576, 596, 766
BssMI GATC 3 cut(s) 219, 658, 793
Bst2UI CCWGG 4 cut(s) 6, 310, 597, 768
Bst4CI ACNGT 1 cut(s) 211
Bst6I CTCTTC 1 cut(s) 434
BstC8I GCNNGC 3 cut(s) 201, 333, 848
BstDEI CTNAG 4 cut(s) 170, 314, 357, 841
BstENI CCTNNNNNAGG 1 cut(s) 313
BstF5I GGATG 1 cut(s) 454
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 3 cut(s) 222, 661, 796
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 3 cut(s) 219, 658, 793
BstMCI CGRYCG 2 cut(s) 200, 222
BstMWI GCNNNNNNNGC 3 cut(s) 417, 734, 833
BstNI CCWGG 4 cut(s) 6, 310, 597, 768
BstSCI CCNGG 6 cut(s) 4, 140, 308, 595, 609, 766
BstV1I GCAGC 4 cut(s) 69, 268, 493, 749
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BstZI CGGCCG 1 cut(s) 197
BsuRI GGCC 4 cut(s) 137, 199, 256, 624
BtsCI GGATG 1 cut(s) 454
BtsIMutI CAGTG 2 cut(s) 203, 761
Cac8I GCNNGC 3 cut(s) 201, 333, 848
CfoI GCGC 1 cut(s) 286
Cfr10I RCCGGY 3 cut(s) 195, 199, 394
Cfr13I GGNCC 4 cut(s) 13, 254, 305, 392
Csp6I GTAC 6 cut(s) 212, 381, 423, 433, 455, 750
CspAI ACCGGT 1 cut(s) 394
CviAII CATG 3 cut(s) 40, 389, 584
CviQI GTAC 6 cut(s) 212, 381, 423, 433, 455, 750
DdeI CTNAG 4 cut(s) 170, 314, 357, 841
DpnI GATC 3 cut(s) 221, 660, 795
DpnII GATC 3 cut(s) 219, 658, 793
DraI TTTAAA 1 cut(s) 808
EaeI YGGCCR 1 cut(s) 197
EagI CGGCCG 1 cut(s) 197
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
Ecl136II GAGCTC 1 cut(s) 294
EclXI CGGCCG 1 cut(s) 197
Eco24I GRGCYC 1 cut(s) 296
Eco47I GGWCC 3 cut(s) 13, 305, 392
Eco52I CGGCCG 1 cut(s) 197
Eco53kI GAGCTC 1 cut(s) 294
Eco57I CTGAAG 1 cut(s) 344
EcoICRI GAGCTC 1 cut(s) 294
EcoNI CCTNNNNNAGG 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 13
EcoRII CCWGG 4 cut(s) 4, 308, 595, 766
EcoT38I GRGCYC 1 cut(s) 296
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 3 cut(s) 43, 392, 587
FalI AAGNNNNNCTT 2 cut(s) 532, 564
FaqI GGGAC 1 cut(s) 26
FatI CATG 3 cut(s) 39, 388, 583
FbaI TGATCA 1 cut(s) 793
Fnu4HI GCNGC 6 cut(s) 83, 257, 282, 287, 507, 738
FokI GGATG 1 cut(s) 461
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 6 cut(s) 83, 257, 282, 287, 507, 738
FspBI CTAG 1 cut(s) 886
GlaI GCGC 1 cut(s) 285
GluI GCNGC 6 cut(s) 83, 257, 282, 287, 507, 738
GsaI CCCAGC 2 cut(s) 255, 817
HaeIII GGCC 4 cut(s) 137, 199, 256, 624
HapII CCGG 5 cut(s) 141, 196, 200, 395, 610
HhaI GCGC 1 cut(s) 286
Hin1II CATG 3 cut(s) 43, 392, 587
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 2 cut(s) 150, 225
HpaII CCGG 5 cut(s) 141, 196, 200, 395, 610
HphI GGTGA 1 cut(s) 172
Hpy166II GTNNAC 4 cut(s) 32, 154, 214, 564
Hpy188I TCNGA 1 cut(s) 224
Hpy188III TCNNGA 4 cut(s) 169, 356, 364, 791
Hpy8I GTNNAC 4 cut(s) 32, 154, 214, 564
HpyAV CCTTC 4 cut(s) 27, 33, 236, 476
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4V TGCA 4 cut(s) 85, 300, 452, 689
HpyF10VI GCNNNNNNNGC 3 cut(s) 417, 734, 833
HpyF3I CTNAG 4 cut(s) 170, 314, 357, 841
Hsp92II CATG 3 cut(s) 43, 392, 587
HspAI GCGC 1 cut(s) 284
KflI GGGWCCC 1 cut(s) 13
KpnI GGTACC 1 cut(s) 753
KroI GCCGGC 1 cut(s) 199
KroNI GCCGGC 1 cut(s) 201
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 3 cut(s) 219, 658, 793
LmnI GCTCC 4 cut(s) 145, 299, 503, 578
Lsp1109I GCAGC 4 cut(s) 69, 268, 493, 749
LweI GCATC 2 cut(s) 394, 439
MaeI CTAG 1 cut(s) 886
MaeIII GTNAC 1 cut(s) 397
MalI GATC 3 cut(s) 221, 660, 795
MboI GATC 3 cut(s) 219, 658, 793
MboII GAAGA 6 cut(s) 116, 119, 337, 451, 537, 668
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 296
MluCI AATT 2 cut(s) 96, 861
MlyI GAGTC 1 cut(s) 159
MmeI TCCRAC 2 cut(s) 216, 493
MroNI GCCGGC 1 cut(s) 199
MseI TTAA 2 cut(s) 555, 807
MslI CAYNNNNRTG 1 cut(s) 237
MspI CCGG 5 cut(s) 141, 196, 200, 395, 610
MspR9I CCNGG 6 cut(s) 6, 142, 310, 597, 611, 768
MvaI CCWGG 4 cut(s) 6, 310, 597, 768
MwoI GCNNNNNNNGC 3 cut(s) 417, 734, 833
NaeI GCCGGC 1 cut(s) 201
NciI CCSGG 2 cut(s) 142, 611
NdeII GATC 3 cut(s) 219, 658, 793
NgoMIV GCCGGC 1 cut(s) 199
NlaIII CATG 3 cut(s) 43, 392, 587
NlaIV GGNNCC 6 cut(s) 14, 15, 307, 320, 354, 751
NmeAIII GCCGAG 2 cut(s) 315, 557
PcsI WCGNNNNNNNCGW 1 cut(s) 215
PdiI GCCGGC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 225
PflMI CCANNNNNTGG 1 cut(s) 349
PinAI ACCGGT 1 cut(s) 394
PkrI GCNGC 6 cut(s) 84, 258, 283, 288, 508, 739
Ple19I CGATCG 1 cut(s) 222
PleI GAGTC 1 cut(s) 158
PpsI GAGTC 1 cut(s) 158
PpuMI RGGWCCY 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 296
Psp5II RGGWCCY 1 cut(s) 13
Psp6I CCWGG 4 cut(s) 4, 308, 595, 766
PspFI CCCAGC 2 cut(s) 251, 813
PspGI CCWGG 4 cut(s) 4, 308, 595, 766
PspN4I GGNNCC 6 cut(s) 14, 15, 307, 320, 354, 751
PspPI GGNCC 4 cut(s) 13, 254, 305, 392
PspPPI RGGWCCY 1 cut(s) 13
PsuI RGATCY 1 cut(s) 658
PvuI CGATCG 1 cut(s) 222
RsaI GTAC 6 cut(s) 213, 382, 424, 434, 456, 751
RsaNI GTAC 6 cut(s) 212, 381, 423, 433, 455, 750
RseI CAYNNNNRTG 1 cut(s) 237
SacI GAGCTC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 555, 807
SatI GCNGC 6 cut(s) 83, 257, 282, 287, 507, 738
Sau3AI GATC 3 cut(s) 219, 658, 793
Sau96I GGNCC 4 cut(s) 13, 254, 305, 392
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 1 cut(s) 159
ScrFI CCNGG 6 cut(s) 6, 142, 310, 597, 611, 768
SduI GDGCHC 1 cut(s) 296
SetI ASST 7 cut(s) 162, 296, 324, 333, 508, 787, 815
SfaNI GCATC 2 cut(s) 394, 439
SinI GGWCC 3 cut(s) 13, 305, 392
SmiMI CAYNNNNRTG 1 cut(s) 237
Sse9I AATT 2 cut(s) 96, 861
SsiI CCGC 5 cut(s) 124, 183, 257, 287, 420
SspMI CTAG 1 cut(s) 886
SstI GAGCTC 1 cut(s) 296
StyD4I CCNGG 6 cut(s) 4, 140, 308, 595, 609, 766
TaaI ACNGT 1 cut(s) 211
TaqI TCGA 1 cut(s) 363
TasI AATT 2 cut(s) 96, 861
TatI WGTACW 2 cut(s) 432, 454
TauI GCSGC 2 cut(s) 259, 289
TfiI GAWTC 1 cut(s) 225
Tru1I TTAA 2 cut(s) 555, 807
Tru9I TTAA 2 cut(s) 555, 807
TscAI CASTG 2 cut(s) 210, 761
TseI GCWGC 4 cut(s) 82, 281, 506, 737
TspRI CASTG 2 cut(s) 210, 761
Van91I CCANNNNNTGG 1 cut(s) 349
VpaK11BI GGWCC 3 cut(s) 13, 305, 392
XagI CCTNNNNNAGG 1 cut(s) 313
XspI CTAG 1 cut(s) 886
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.