Rh4BG364900

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008523
Physical Location & Seq
Reverse (-)
52248805 .. 52251963
3159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG364900.1

Sequence Viewer

Length: 1056 bp
ATGCTAGCCAGAACGACGCCGTCTCCACTGCTCACTTTTGCCACTACAAAACTGGTGTGCTGCTGCTTCCACAGTTTGGCACCAAAAAACTCACCAAACAAGATGGTGAAGCTGATCGTGGCGACGACGACCGATCCGGCCTCCGTCAACCCTGCCAATGCTCTCTTATCCAAGCCCGGCTGGTCCCCAACCCCTTCTCTTCTACAGGAGGATATGAAGAGCTTCGCTAACGGAGCCGTTAGGGTTTTGCTGCACAGCAGGAGCATCATCGAAGAGGACGATTTGGACGCTCGCTGGGAACAAGCCACCGGCGAACACGTGGACGAGCTCATCTTCTTGAGCAAGCACAAAGCCGCTTCCAGCCGCCCTGCGCTTACTGTTCACCCCATCGGGGTTCCTCATTTGCGAGACGGCGACGTTTTGCCGCAGGGCGGGAAGCCGGGCTGGGCCGCGCCGCCGAATCCGAGGATTGGGCCCTGGCTCAGGCTCTTGAAGAGAATCGCGCAAGATCACAACTTGGTCCCTGAGTTTGAGGTGACTTTGGAGGCTACACATCATGGACCGATTACAGACACGCCTGCCATGTTCCTGGAGATTGGCAGTACAGATGATTGCTGGAAGAGGCAGGATGCAGCTGAAGTTATGGCTCTATTAGTTTGGGAAGGACTTGGGCTTGGAGGGGGAGCTGCTGTTGGAAACTGGAACGGGGGGAACGATAATAAAAGAGTTCTGCTTGGGATAGGTGGTGGACATTATGTACCTCGTCATATGGATATAGCTCTGAAAGATGATGTCTGGGTGGGCCATCTAATTTCGGGTTATTCGTTACCAATGGAAGATCCGAGCCAGTCCAAGACAGAAACAAATACAAAAGATATCGGTGGAACTTGGAAACAATCAATCAAAGTAGCATTTGAGGCTACGCAATCGGCTTTCCCTGGTGGAGAAATTGTAGCTCATCTTGATCACAAGAGTTTCAAAAGCTGGCAAAAGAATGCTATAACGAGTTTCCTAGAGGAGCAGAACATTACAATTAGCAAACGTGATTTCTTCTGA

Protein Analysis

351

Amino Acids

38.27

Weight (kDa)

6.27

Isoelectric Point (pI)

49.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 104 - 345 4.5e-58 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 79
AccB7I CCANNNNNTGG 1 cut(s) 76
AccII CGCG 2 cut(s) 452, 503
AciI CCGC 6 cut(s) 354, 364, 425, 432, 450, 455
AclWI GGATC 2 cut(s) 128, 833
AcuI CTGAAG 1 cut(s) 657
AcvI CACGTG 1 cut(s) 319
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 2 cut(s) 604, 759
AfiI CCNNNNNNNGG 5 cut(s) 76, 391, 431, 470, 483
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 2 cut(s) 493, 979
AjnI CCWGG 3 cut(s) 476, 588, 937
AluBI AGCT 8 cut(s) 112, 222, 328, 635, 686, 779, 956, 984
AluI AGCT 8 cut(s) 112, 222, 328, 635, 686, 779, 956, 984
Alw21I GWGCWC 1 cut(s) 330
Alw26I GTCTC 2 cut(s) 27, 402
AlwI GGATC 2 cut(s) 128, 833
AoxI GGCC 4 cut(s) 138, 447, 473, 802
ApaI GGGCCC 1 cut(s) 477
ApeKI GCWGC 5 cut(s) 60, 63, 250, 632, 686
Asp700I GAANNNNTTC 1 cut(s) 221
AspLEI GCGC 3 cut(s) 373, 454, 505
AspS9I GGNCC 7 cut(s) 183, 447, 473, 474, 520, 560, 802
AsuC2I CCSGG 2 cut(s) 177, 441
AsuHPI GGTGA 4 cut(s) 84, 118, 374, 547
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 3 cut(s) 183, 520, 560
BaeGI GKGCMC 1 cut(s) 477
BanI GGYRCC 1 cut(s) 79
BanII GRGCYC 2 cut(s) 330, 477
BbrPI CACGTG 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 330
BbvI GCAGC 5 cut(s) 47, 50, 237, 644, 673
BccI CCATC 3 cut(s) 97, 395, 813
BceAI ACGGC 3 cut(s) 4, 221, 427
BciT130I CCWGG 3 cut(s) 478, 590, 939
BclI TGATCA 1 cut(s) 964
BcnI CCSGG 2 cut(s) 177, 441
BcoDI GTCTC 2 cut(s) 27, 402
BfaI CTAG 2 cut(s) 5, 1013
BfmI CTRYAG 1 cut(s) 203
Bme1390I CCNGG 5 cut(s) 177, 441, 478, 590, 939
Bme18I GGWCC 3 cut(s) 183, 520, 560
BmgT120I GGNCC 7 cut(s) 183, 447, 473, 474, 520, 560, 802
BmiI GGNNCC 6 cut(s) 81, 185, 235, 396, 475, 522
BmrFI CCNGG 5 cut(s) 177, 441, 478, 590, 939
BmsI GCATC 2 cut(s) 273, 619
BmtI GCTAGC 1 cut(s) 8
BpmI CTGGAG 1 cut(s) 611
Bpu10I CCTNAGC 1 cut(s) 482
BpuEI CTTGAG 1 cut(s) 358
BpuMI CCSGG 2 cut(s) 177, 441
BsaAI YACGTR 1 cut(s) 319
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 464, 476, 937
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 675, 705, 936, 966
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 391, 431, 470, 483
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 3 cut(s) 57, 704, 847
BseBI CCWGG 3 cut(s) 478, 590, 939
BseDI CCNNGG 3 cut(s) 464, 476, 937
BseGI GGATG 1 cut(s) 634
BseLI CCNNNNNNNGG 5 cut(s) 76, 391, 431, 470, 483
BseMII CTCAG 2 cut(s) 496, 516
BseNI ACTGG 3 cut(s) 57, 704, 847
BseRI GAGGAG 1 cut(s) 1031
BseSI GKGCMC 1 cut(s) 477
BseXI GCAGC 5 cut(s) 47, 50, 237, 644, 673
BseYI CCCAGC 2 cut(s) 294, 444
BsgI GTGCAG 1 cut(s) 236
Bsh1236I CGCG 2 cut(s) 452, 503
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 4 cut(s) 140, 449, 475, 804
BshNI GGYRCC 1 cut(s) 79
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 330
BsiSI CCGG 4 cut(s) 137, 177, 309, 440
BslFI GGGAC 2 cut(s) 169, 506
BslI CCNNNNNNNGG 5 cut(s) 76, 391, 431, 470, 483
BsmAI GTCTC 2 cut(s) 27, 402
BsmBI CGTCTC 2 cut(s) 27, 402
BsmFI GGGAC 2 cut(s) 169, 506
BsmI GAATGC 1 cut(s) 1000
BsnI GGCC 4 cut(s) 140, 449, 475, 804
Bsp120I GGGCCC 1 cut(s) 473
Bsp1286I GDGCHC 2 cut(s) 330, 477
Bsp143I GATC 5 cut(s) 114, 133, 508, 838, 964
BspACI CCGC 6 cut(s) 354, 364, 425, 432, 450, 455
BspANI GGCC 4 cut(s) 140, 449, 475, 804
BspCNI CTCAG 2 cut(s) 495, 517
BspFNI CGCG 2 cut(s) 452, 503
BspLI GGNNCC 6 cut(s) 81, 185, 235, 396, 475, 522
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 128, 833
BspQI GCTCTTC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 79
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 3 cut(s) 57, 704, 847
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 3 cut(s) 464, 476, 937
BssMI GATC 5 cut(s) 114, 133, 508, 838, 964
BssNI GRCGYC 1 cut(s) 17
Bst2UI CCWGG 3 cut(s) 478, 590, 939
Bst4CI ACNGT 2 cut(s) 74, 379
Bst6I CTCTTC 5 cut(s) 204, 212, 267, 488, 614
BstACI GRCGYC 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 319
BstC8I GCNNGC 5 cut(s) 6, 292, 344, 579, 986
BstDEI CTNAG 2 cut(s) 482, 525
BstENI CCTNNNNNAGG 1 cut(s) 481
BstF5I GGATG 1 cut(s) 634
BstFNI CGCG 2 cut(s) 452, 503
BstHHI GCGC 3 cut(s) 373, 454, 505
BstKTI GATC 5 cut(s) 117, 136, 511, 841, 967
BstMAI GTCTC 2 cut(s) 27, 402
BstMBI GATC 5 cut(s) 114, 133, 508, 838, 964
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 233, 917
BstNI CCWGG 3 cut(s) 478, 590, 939
BstSCI CCNGG 5 cut(s) 175, 439, 476, 588, 937
BstSFI CTRYAG 1 cut(s) 203
BstSLI GKGCMC 1 cut(s) 477
BstUI CGCG 2 cut(s) 452, 503
BstV1I GCAGC 5 cut(s) 47, 50, 237, 644, 673
BstX2I RGATCY 1 cut(s) 838
BstXI CCANNNNNNTGG 1 cut(s) 589
BstYI RGATCY 1 cut(s) 838
BsuRI GGCC 4 cut(s) 140, 449, 475, 804
BtsCI GGATG 1 cut(s) 634
BtsI GCAGTG 1 cut(s) 26
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 5 cut(s) 6, 292, 344, 579, 986
CfoI GCGC 3 cut(s) 373, 454, 505
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 7 cut(s) 183, 447, 473, 474, 520, 560, 802
CseI GACGC 2 cut(s) 25, 296
Csp6I GTAC 2 cut(s) 603, 758
CviAII CATG 2 cut(s) 557, 583
CviQI GTAC 2 cut(s) 603, 758
DdeI CTNAG 2 cut(s) 482, 525
DpnI GATC 5 cut(s) 116, 135, 510, 840, 966
DpnII GATC 5 cut(s) 114, 133, 508, 838, 964
Eam1104I CTCTTC 5 cut(s) 204, 212, 267, 488, 614
EarI CTCTTC 5 cut(s) 204, 212, 267, 488, 614
Ecl136II GAGCTC 1 cut(s) 328
Eco24I GRGCYC 2 cut(s) 330, 477
Eco32I GATATC 1 cut(s) 877
Eco47I GGWCC 3 cut(s) 183, 520, 560
Eco53kI GAGCTC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 657
Eco72I CACGTG 1 cut(s) 319
EcoICRI GAGCTC 1 cut(s) 328
EcoNI CCTNNNNNAGG 1 cut(s) 481
EcoO109I RGGNCCY 1 cut(s) 474
EcoRII CCWGG 3 cut(s) 476, 588, 937
EcoRV GATATC 1 cut(s) 877
EcoT38I GRGCYC 2 cut(s) 330, 477
Esp3I CGTCTC 2 cut(s) 27, 402
FaeI CATG 2 cut(s) 560, 586
FaiI YATR 9 cut(s) 215, 558, 584, 644, 756, 768, 770, 776, 1001
FaqI GGGAC 2 cut(s) 169, 506
FatI CATG 2 cut(s) 556, 582
FauI CCCGC 1 cut(s) 425
FauNDI CATATG 1 cut(s) 768
FbaI TGATCA 1 cut(s) 964
FokI GGATG 1 cut(s) 641
FriOI GRGCYC 2 cut(s) 330, 477
FspBI CTAG 2 cut(s) 5, 1013
GlaI GCGC 3 cut(s) 372, 453, 504
GsaI CCCAGC 2 cut(s) 298, 448
GsuI CTGGAG 1 cut(s) 611
HaeIII GGCC 4 cut(s) 140, 449, 475, 804
HapII CCGG 4 cut(s) 137, 177, 309, 440
HgaI GACGC 2 cut(s) 25, 296
HhaI GCGC 3 cut(s) 373, 454, 505
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 2 cut(s) 560, 586
Hin6I GCGC 3 cut(s) 371, 452, 503
HinP1I GCGC 3 cut(s) 371, 452, 503
HincII GTYRAC 1 cut(s) 148
HindII GTYRAC 1 cut(s) 148
HinfI GANTC 2 cut(s) 460, 498
HpaII CCGG 4 cut(s) 137, 177, 309, 440
HphI GGTGA 4 cut(s) 84, 118, 374, 547
Hpy166II GTNNAC 4 cut(s) 148, 322, 382, 749
Hpy188I TCNGA 4 cut(s) 465, 783, 843, 1055
Hpy188III TCNNGA 3 cut(s) 337, 490, 962
Hpy8I GTNNAC 4 cut(s) 148, 322, 382, 749
Hpy99I CGWCG 4 cut(s) 19, 127, 130, 419
HpyAV CCTTC 2 cut(s) 204, 656
HpyCH4III ACNGT 2 cut(s) 74, 379
HpyCH4IV ACGT 3 cut(s) 318, 417, 1042
HpyCH4V TGCA 2 cut(s) 253, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 917
HpyF3I CTNAG 2 cut(s) 482, 525
HpySE526I ACGT 3 cut(s) 318, 417, 1042
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 2 cut(s) 560, 586
HspAI GCGC 3 cut(s) 371, 452, 503
Ksp22I TGATCA 1 cut(s) 964
Kzo9I GATC 5 cut(s) 114, 133, 508, 838, 964
LguI GCTCTTC 1 cut(s) 212
LmnI GCTCC 4 cut(s) 233, 261, 683, 1018
Lsp1109I GCAGC 5 cut(s) 47, 50, 237, 644, 673
LweI GCATC 2 cut(s) 273, 619
MaeI CTAG 2 cut(s) 5, 1013
MaeII ACGT 3 cut(s) 318, 417, 1042
MaeIII GTNAC 2 cut(s) 535, 825
MalI GATC 5 cut(s) 116, 135, 510, 840, 966
MboI GATC 5 cut(s) 114, 133, 508, 838, 964
MboII GAAGA 8 cut(s) 191, 229, 284, 325, 505, 631, 848, 1042
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 2 cut(s) 330, 477
MluCI AATT 3 cut(s) 810, 948, 1032
MmeI TCCRAC 1 cut(s) 673
MroXI GAANNNNTTC 1 cut(s) 221
MspA1I CMGCKG 1 cut(s) 635
MspI CCGG 4 cut(s) 137, 177, 309, 440
MspR9I CCNGG 5 cut(s) 177, 441, 478, 590, 939
Mva1269I GAATGC 1 cut(s) 1000
MvaI CCWGG 3 cut(s) 478, 590, 939
MvnI CGCG 2 cut(s) 452, 503
MwoI GCNNNNNNNGC 2 cut(s) 233, 917
NciI CCSGG 2 cut(s) 177, 441
NdeI CATATG 1 cut(s) 768
NdeII GATC 5 cut(s) 114, 133, 508, 838, 964
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 2 cut(s) 560, 586
NlaIV GGNNCC 6 cut(s) 81, 185, 235, 396, 475, 522
NmuCI GTSAC 1 cut(s) 535
PciSI GCTCTTC 1 cut(s) 212
PcsI WCGNNNNNNNCGW 3 cut(s) 276, 711, 821
PctI GAATGC 1 cut(s) 1000
PdmI GAANNNNTTC 1 cut(s) 221
PfeI GAWTC 2 cut(s) 460, 498
PflFI GACNNNGTC 1 cut(s) 19
PflMI CCANNNNNTGG 1 cut(s) 76
PfoI TCCNGGA 1 cut(s) 588
PmaCI CACGTG 1 cut(s) 319
PmlI CACGTG 1 cut(s) 319
Ppu21I YACGTR 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 3 cut(s) 476, 588, 937
PspCI CACGTG 1 cut(s) 319
PspFI CCCAGC 2 cut(s) 294, 444
PspGI CCWGG 3 cut(s) 476, 588, 937
PspN4I GGNNCC 6 cut(s) 81, 185, 235, 396, 475, 522
PspOMI GGGCCC 1 cut(s) 473
PspPI GGNCC 7 cut(s) 183, 447, 473, 474, 520, 560, 802
PsuI RGATCY 1 cut(s) 838
PsyI GACNNNGTC 1 cut(s) 19
PvuII CAGCTG 1 cut(s) 635
RsaI GTAC 2 cut(s) 604, 759
RsaNI GTAC 2 cut(s) 603, 758
SacI GAGCTC 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 212
Sau3AI GATC 5 cut(s) 114, 133, 508, 838, 964
Sau96I GGNCC 7 cut(s) 183, 447, 473, 474, 520, 560, 802
ScrFI CCNGG 5 cut(s) 177, 441, 478, 590, 939
SduI GDGCHC 2 cut(s) 330, 477
SfaNI GCATC 2 cut(s) 273, 619
SfcI CTRYAG 1 cut(s) 203
SgrAI CRCCGGYG 1 cut(s) 308
SinI GGWCC 3 cut(s) 183, 520, 560
SmlI CTYRAG 1 cut(s) 337
SmoI CTYRAG 1 cut(s) 337
Sse9I AATT 3 cut(s) 810, 948, 1032
SsiI CCGC 6 cut(s) 354, 364, 425, 432, 450, 455
SspMI CTAG 2 cut(s) 5, 1013
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 5 cut(s) 175, 439, 476, 588, 937
TaaI ACNGT 2 cut(s) 74, 379
TaiI ACGT 3 cut(s) 321, 420, 1045
TaqI TCGA 1 cut(s) 270
TaqII GACCGA 2 cut(s) 146, 577
TasI AATT 3 cut(s) 810, 948, 1032
TatI WGTACW 1 cut(s) 602
TauI GCSGC 5 cut(s) 356, 366, 427, 452, 457
TfiI GAWTC 2 cut(s) 460, 498
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 5 cut(s) 60, 63, 250, 632, 686
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 1 cut(s) 230
TspGWI ACGGA 2 cut(s) 133, 246
TspRI CASTG 1 cut(s) 33
Tth111I GACNNNGTC 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 76
VpaK11BI GGWCC 3 cut(s) 183, 520, 560
XagI CCTNNNNNAGG 1 cut(s) 481
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XmnI GAANNNNTTC 1 cut(s) 221
XspI CTAG 2 cut(s) 5, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.