Rw4G031150

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008523
Physical Location & Seq
Reverse (-)
58005592 .. 58008430
2839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031150.1

Sequence Viewer

Length: 951 bp
ATGGTGAAGCTGATCGTGGCGACGACGACCGATCCGGCCTCCGTCAACCCTGCCAATGCTCTCTTATCCAAGCCCGGCTGGTCCCCAACCGCTTCTCTTCTACAGGATATGAAGAGCTTCGCTAACGGAGACGTTAGGGTTTTGCTGCACAACAGGAGCATAATCGAAGAGGACGACTTGGACGCTCGCTGGGAACAAGCCACCGGCGAACACGTGGACGAGCTCATCTTCTTGAGCAAGCACAAAGCCGCTTCCAGCCGCCCTGCGCTTACTGTTCACCCCATCGGGGTTCCTCATTTGCGAAACGGCGACGTTTTGCCGCAGGGCGGGAAGCCGGGCTGGGCCGCGCCGCCGAATCCGAGGATTGGGCCCTGGCTCAGGCTCTTGAAGAGAATCGCGCAGGATCGCAACTTGGTCCCTGAGTTTGAGGTGACTTTGGAGGCTACACATCATGGACCGATTACAGACACGCCTGCCATGTTCCTGGAGATTGGCAGCTCAGATGATTGCTGGAAGAGGCAGGATGCAGCTGAAGTTATGGCTCTATTAGTTTGGGAAGGACTTGGGCTTGGAGGGGGAGCTGCTGTTGGAAACTGGAACGGGGGGAACGATAATAAAAGAGTTCTGCTTGGGATAGGTGGTGGACATTATGTACCTCGTCATATGGATATAGCTCTGAAAGATGATGTCTGGGTGGGCCATCTAATTTCGGGTTATTCGTTACCAATGGAAGATCCGAGCCAGTCCAAGACAGAAACAAATACAAAAGATATCGGTGGAACTTGGAAACAATCAATCAAAGTAGCATTTGAGGCTACGCAATCGGCTTTCCCTGGTGGAGAAATTGTAGCTCATCTTGATCACAAGAGTTTCAAAAGCTGGCAAAAGAATGCCATAACGAGTTTCCTAGAGGAGCAGAACATTACAATTAGCAAACGCGATTTCTTCTGA

Protein Analysis

316

Amino Acids

34.52

Weight (kDa)

5.94

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 69 - 310 3.3e-58 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 347, 398, 939
AciI CCGC 7 cut(s) 90, 249, 259, 320, 327, 345, 350
AclWI GGATC 3 cut(s) 26, 411, 728
AcuI CTGAAG 1 cut(s) 552
AcvI CACGTG 1 cut(s) 214
AfaI GTAC 1 cut(s) 654
AfiI CCNNNNNNNGG 4 cut(s) 286, 326, 365, 378
AflIII ACRYGT 1 cut(s) 211
AgsI TTSAA 2 cut(s) 388, 874
AjnI CCWGG 3 cut(s) 371, 483, 832
AluBI AGCT 9 cut(s) 10, 117, 223, 498, 530, 581, 674, 851, 879
AluI AGCT 9 cut(s) 10, 117, 223, 498, 530, 581, 674, 851, 879
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 123
AlwI GGATC 3 cut(s) 26, 411, 728
AoxI GGCC 4 cut(s) 36, 342, 368, 697
ApaI GGGCCC 1 cut(s) 372
ApeKI GCWGC 4 cut(s) 145, 495, 527, 581
Asp700I GAANNNNTTC 1 cut(s) 116
AspLEI GCGC 3 cut(s) 268, 349, 400
AspS9I GGNCC 7 cut(s) 81, 342, 368, 369, 415, 455, 697
AsuC2I CCSGG 2 cut(s) 75, 336
AsuHPI GGTGA 3 cut(s) 16, 269, 442
AvaII GGWCC 3 cut(s) 81, 415, 455
BaeGI GKGCMC 1 cut(s) 372
BanII GRGCYC 2 cut(s) 225, 372
BbrPI CACGTG 1 cut(s) 214
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 4 cut(s) 132, 507, 539, 568
BccI CCATC 2 cut(s) 290, 708
BceAI ACGGC 1 cut(s) 322
BciT130I CCWGG 3 cut(s) 373, 485, 834
BclI TGATCA 1 cut(s) 859
BcnI CCSGG 2 cut(s) 75, 336
BcoDI GTCTC 1 cut(s) 123
BfaI CTAG 1 cut(s) 908
BfmI CTRYAG 1 cut(s) 101
BisI GCNGC 9 cut(s) 146, 249, 259, 320, 345, 350, 496, 528, 582
BlsI GCNGC 9 cut(s) 147, 250, 260, 321, 346, 351, 497, 529, 583
Bme1390I CCNGG 5 cut(s) 75, 336, 373, 485, 834
Bme18I GGWCC 3 cut(s) 81, 415, 455
BmgT120I GGNCC 7 cut(s) 81, 342, 368, 369, 415, 455, 697
BmiI GGNNCC 4 cut(s) 83, 291, 370, 417
BmrFI CCNGG 5 cut(s) 75, 336, 373, 485, 834
BmsI GCATC 1 cut(s) 514
BpmI CTGGAG 1 cut(s) 506
Bpu10I CCTNAGC 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 253
BpuMI CCSGG 2 cut(s) 75, 336
BsaAI YACGTR 1 cut(s) 214
BsaJI CCNNGG 3 cut(s) 359, 371, 832
BsaXI ACNNNNNCTCC 4 cut(s) 570, 600, 831, 861
Bsc4I CCNNNNNNNGG 4 cut(s) 286, 326, 365, 378
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 599, 742
BseBI CCWGG 3 cut(s) 373, 485, 834
BseDI CCNNGG 3 cut(s) 359, 371, 832
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 4 cut(s) 286, 326, 365, 378
BseMII CTCAG 3 cut(s) 391, 411, 513
BseNI ACTGG 2 cut(s) 599, 742
BseRI GAGGAG 1 cut(s) 926
BseSI GKGCMC 1 cut(s) 372
BseXI GCAGC 4 cut(s) 132, 507, 539, 568
BseYI CCCAGC 2 cut(s) 189, 339
BsgI GTGCAG 1 cut(s) 131
Bsh1236I CGCG 3 cut(s) 347, 398, 939
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 4 cut(s) 38, 344, 370, 699
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 225
BsiSI CCGG 4 cut(s) 35, 75, 204, 335
BslFI GGGAC 2 cut(s) 67, 401
BslI CCNNNNNNNGG 4 cut(s) 286, 326, 365, 378
BsmAI GTCTC 1 cut(s) 123
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 2 cut(s) 67, 401
BsmI GAATGC 1 cut(s) 895
BsnI GGCC 4 cut(s) 38, 344, 370, 699
Bsp120I GGGCCC 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 225, 372
Bsp143I GATC 5 cut(s) 12, 31, 403, 733, 859
BspACI CCGC 7 cut(s) 90, 249, 259, 320, 327, 345, 350
BspANI GGCC 4 cut(s) 38, 344, 370, 699
BspCNI CTCAG 3 cut(s) 390, 412, 512
BspFNI CGCG 3 cut(s) 347, 398, 939
BspLI GGNNCC 4 cut(s) 83, 291, 370, 417
BspPI GGATC 3 cut(s) 26, 411, 728
BspQI GCTCTTC 1 cut(s) 107
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 2 cut(s) 599, 742
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 3 cut(s) 359, 371, 832
BssMI GATC 5 cut(s) 12, 31, 403, 733, 859
Bst2UI CCWGG 3 cut(s) 373, 485, 834
Bst4CI ACNGT 1 cut(s) 274
Bst6I CTCTTC 5 cut(s) 102, 107, 162, 383, 509
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 4 cut(s) 187, 239, 474, 881
BstDEI CTNAG 3 cut(s) 377, 420, 499
BstENI CCTNNNNNAGG 1 cut(s) 376
BstF5I GGATG 1 cut(s) 529
BstFNI CGCG 3 cut(s) 347, 398, 939
BstHHI GCGC 3 cut(s) 268, 349, 400
BstKTI GATC 5 cut(s) 15, 34, 406, 736, 862
BstMAI GTCTC 1 cut(s) 123
BstMBI GATC 5 cut(s) 12, 31, 403, 733, 859
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 1 cut(s) 812
BstNI CCWGG 3 cut(s) 373, 485, 834
BstSCI CCNGG 5 cut(s) 73, 334, 371, 483, 832
BstSFI CTRYAG 1 cut(s) 101
BstSLI GKGCMC 1 cut(s) 372
BstUI CGCG 3 cut(s) 347, 398, 939
BstV1I GCAGC 4 cut(s) 132, 507, 539, 568
BstX2I RGATCY 1 cut(s) 733
BstXI CCANNNNNNTGG 1 cut(s) 484
BstYI RGATCY 1 cut(s) 733
BsuRI GGCC 4 cut(s) 38, 344, 370, 699
BtsCI GGATG 1 cut(s) 529
Cac8I GCNNGC 4 cut(s) 187, 239, 474, 881
CfoI GCGC 3 cut(s) 268, 349, 400
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 7 cut(s) 81, 342, 368, 369, 415, 455, 697
CseI GACGC 1 cut(s) 191
Csp6I GTAC 1 cut(s) 653
CviAII CATG 2 cut(s) 452, 478
CviQI GTAC 1 cut(s) 653
DdeI CTNAG 3 cut(s) 377, 420, 499
DpnI GATC 5 cut(s) 14, 33, 405, 735, 861
DpnII GATC 5 cut(s) 12, 31, 403, 733, 859
Eam1104I CTCTTC 5 cut(s) 102, 107, 162, 383, 509
EarI CTCTTC 5 cut(s) 102, 107, 162, 383, 509
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 2 cut(s) 225, 372
Eco32I GATATC 1 cut(s) 772
Eco47I GGWCC 3 cut(s) 81, 415, 455
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 552
Eco72I CACGTG 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 369
EcoRII CCWGG 3 cut(s) 371, 483, 832
EcoRV GATATC 1 cut(s) 772
EcoT38I GRGCYC 2 cut(s) 225, 372
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 2 cut(s) 455, 481
FaqI GGGAC 2 cut(s) 67, 401
FatI CATG 2 cut(s) 451, 477
FauI CCCGC 1 cut(s) 320
FauNDI CATATG 1 cut(s) 663
FbaI TGATCA 1 cut(s) 859
Fnu4HI GCNGC 9 cut(s) 146, 249, 259, 320, 345, 350, 496, 528, 582
FokI GGATG 1 cut(s) 536
FriOI GRGCYC 2 cut(s) 225, 372
Fsp4HI GCNGC 9 cut(s) 146, 249, 259, 320, 345, 350, 496, 528, 582
FspBI CTAG 1 cut(s) 908
GlaI GCGC 3 cut(s) 267, 348, 399
GluI GCNGC 9 cut(s) 146, 249, 259, 320, 345, 350, 496, 528, 582
GsaI CCCAGC 2 cut(s) 193, 343
GsuI CTGGAG 1 cut(s) 506
HaeIII GGCC 4 cut(s) 38, 344, 370, 699
HapII CCGG 4 cut(s) 35, 75, 204, 335
HgaI GACGC 1 cut(s) 191
HhaI GCGC 3 cut(s) 268, 349, 400
Hin1II CATG 2 cut(s) 455, 481
Hin6I GCGC 3 cut(s) 266, 347, 398
HinP1I GCGC 3 cut(s) 266, 347, 398
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 2 cut(s) 355, 393
HpaII CCGG 4 cut(s) 35, 75, 204, 335
HphI GGTGA 3 cut(s) 16, 269, 442
Hpy166II GTNNAC 4 cut(s) 46, 217, 277, 644
Hpy188I TCNGA 5 cut(s) 360, 502, 678, 738, 950
Hpy188III TCNNGA 3 cut(s) 232, 385, 857
Hpy8I GTNNAC 4 cut(s) 46, 217, 277, 644
Hpy99I CGWCG 3 cut(s) 25, 28, 314
HpyAV CCTTC 1 cut(s) 551
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4IV ACGT 3 cut(s) 132, 213, 312
HpyCH4V TGCA 2 cut(s) 148, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 812
HpyF3I CTNAG 3 cut(s) 377, 420, 499
HpySE526I ACGT 3 cut(s) 132, 213, 312
Hsp92II CATG 2 cut(s) 455, 481
HspAI GCGC 3 cut(s) 266, 347, 398
Ksp22I TGATCA 1 cut(s) 859
Kzo9I GATC 5 cut(s) 12, 31, 403, 733, 859
LguI GCTCTTC 1 cut(s) 107
LmnI GCTCC 3 cut(s) 156, 578, 913
Lsp1109I GCAGC 4 cut(s) 132, 507, 539, 568
LweI GCATC 1 cut(s) 514
MaeI CTAG 1 cut(s) 908
MaeII ACGT 3 cut(s) 132, 213, 312
MaeIII GTNAC 2 cut(s) 430, 720
MalI GATC 5 cut(s) 14, 33, 405, 735, 861
MboI GATC 5 cut(s) 12, 31, 403, 733, 859
MboII GAAGA 8 cut(s) 89, 124, 179, 220, 400, 526, 743, 937
MflI RGATCY 1 cut(s) 733
MhlI GDGCHC 2 cut(s) 225, 372
MluCI AATT 3 cut(s) 705, 843, 927
MmeI TCCRAC 1 cut(s) 568
MroXI GAANNNNTTC 1 cut(s) 116
MspA1I CMGCKG 1 cut(s) 530
MspI CCGG 4 cut(s) 35, 75, 204, 335
MspR9I CCNGG 5 cut(s) 75, 336, 373, 485, 834
Mva1269I GAATGC 1 cut(s) 895
MvaI CCWGG 3 cut(s) 373, 485, 834
MvnI CGCG 3 cut(s) 347, 398, 939
MwoI GCNNNNNNNGC 1 cut(s) 812
NciI CCSGG 2 cut(s) 75, 336
NdeI CATATG 1 cut(s) 663
NdeII GATC 5 cut(s) 12, 31, 403, 733, 859
NlaIII CATG 2 cut(s) 455, 481
NlaIV GGNNCC 4 cut(s) 83, 291, 370, 417
NmuCI GTSAC 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 107
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 606, 716
PctI GAATGC 1 cut(s) 895
PdmI GAANNNNTTC 1 cut(s) 116
PfeI GAWTC 2 cut(s) 355, 393
PfoI TCCNGGA 1 cut(s) 483
PkrI GCNGC 9 cut(s) 147, 250, 260, 321, 346, 351, 497, 529, 583
PmaCI CACGTG 1 cut(s) 214
PmlI CACGTG 1 cut(s) 214
Ppu21I YACGTR 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 3 cut(s) 371, 483, 832
PspCI CACGTG 1 cut(s) 214
PspFI CCCAGC 2 cut(s) 189, 339
PspGI CCWGG 3 cut(s) 371, 483, 832
PspN4I GGNNCC 4 cut(s) 83, 291, 370, 417
PspOMI GGGCCC 1 cut(s) 368
PspPI GGNCC 7 cut(s) 81, 342, 368, 369, 415, 455, 697
PsuI RGATCY 1 cut(s) 733
PvuII CAGCTG 1 cut(s) 530
RsaI GTAC 1 cut(s) 654
RsaNI GTAC 1 cut(s) 653
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 107
SatI GCNGC 9 cut(s) 146, 249, 259, 320, 345, 350, 496, 528, 582
Sau3AI GATC 5 cut(s) 12, 31, 403, 733, 859
Sau96I GGNCC 7 cut(s) 81, 342, 368, 369, 415, 455, 697
ScrFI CCNGG 5 cut(s) 75, 336, 373, 485, 834
SduI GDGCHC 2 cut(s) 225, 372
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 101
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 3 cut(s) 81, 415, 455
SmlI CTYRAG 1 cut(s) 232
SmoI CTYRAG 1 cut(s) 232
Sse9I AATT 3 cut(s) 705, 843, 927
SsiI CCGC 7 cut(s) 90, 249, 259, 320, 327, 345, 350
SspMI CTAG 1 cut(s) 908
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 5 cut(s) 73, 334, 371, 483, 832
TaaI ACNGT 1 cut(s) 274
TaiI ACGT 3 cut(s) 135, 216, 315
TaqI TCGA 1 cut(s) 165
TaqII GACCGA 2 cut(s) 44, 472
TasI AATT 3 cut(s) 705, 843, 927
TauI GCSGC 5 cut(s) 251, 261, 322, 347, 352
TfiI GAWTC 2 cut(s) 355, 393
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 4 cut(s) 145, 495, 527, 581
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 1 cut(s) 125
TspGWI ACGGA 2 cut(s) 31, 141
VpaK11BI GGWCC 3 cut(s) 81, 415, 455
XagI CCTNNNNNAGG 1 cut(s) 376
XmnI GAANNNNTTC 1 cut(s) 116
XspI CTAG 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.