FvH4_4g29300

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008523
Physical Location & Seq
Reverse (-)
29485536 .. 29488898
3363 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29300.t1

Sequence Viewer

Length: 948 bp
ATGGTGATTCTGATAGTGGCGACGTCGACCGACCCCGCCTCCATCAACCCGGCCAATGCTCTCCTATCCAAGCCCGGCTGGGCCCCCACCCCTTCTCTACAGGACATGAAAGGCTTCGCTAACGGCGCCGTTAGGGTTTTACTGCACAACAGCAGCATCATCGAAGAGGACGACTTGGACGCTCGCTGGAGAGAAGCCACCGGCGAACACGTGGACGAGCTCATCTTCTTGAGCAAGCACAAGGCCGTTTCCAGCCGTCCTGCGCTTACTGTTCATCCGATCGGGGTTCCTCATTTGCGAAACGGCGACGTTTTGCCGCAGGGCGGGAAGCCGGGATGGGCCGCGCCGCCGAATCCGCGGATTGGGCCCTGGCTTAGGCTATTGAAGAGGATTGCTCAAGATCATGGCTTGGTTCCTGAGTTTGAGGTAACTTTGGAGGCTACACATCATGGACCGATCACTGATACGCCTGCTATGTTTCTGGAGATTGGCAGTACAGATGAGTGCTGGAAGAGGCAGGATGCAGCTGAAGTTATGGCTCTATTAGTTTGGGAAGGACTTGGGCTTGGAGGGGGAGCTGCTGTTGGAAACTGGAACGGGGAGAACCCTAATAAAAAAGTTCTTCTTGGGATAGGTGGTGGACATTATGCACCTCGTCATATGGATATTGCTCTGAAAGATAATGTCTGGGTGGGCCATCTACTTTCGGGTTATTCCTTACCAATGGAAGATCCAAGCCAGTCCAAGACAGAAATAAATACAAAGGATATAGGTGGAACTTGGAAACAAGCAATCAAAGTAGCATTTGAGGTTACGCAAATGGCTTTCCCTGGTGGAGAAATCCTAGCTCATCTTGATCACAAGAGTTTCAAAAGCTGGCAAAAGAATGCTATAACGAAATTCCTAGAGGAGCAGAATATTACAGTTAGCAAACGTGATTTCTTCTGA

Protein Analysis

316

Amino Acids

34.37

Weight (kDa)

6.11

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 68 - 309 1.6e-58 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 125
AccI GTMKAC 1 cut(s) 26
AccII CGCG 2 cut(s) 344, 358
AciI CCGC 7 cut(s) 36, 317, 324, 342, 347, 356, 358
AclWI GGATC 1 cut(s) 725
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 1 cut(s) 899
AcuI CTGAAG 1 cut(s) 549
AcvI CACGTG 1 cut(s) 211
AcyI GRCGYC 2 cut(s) 23, 126
AfaI GTAC 1 cut(s) 496
AfiI CCNNNNNNNGG 3 cut(s) 323, 362, 375
AflIII ACRYGT 1 cut(s) 208
AgsI TTSAA 2 cut(s) 385, 871
AjnI CCWGG 2 cut(s) 368, 829
AluBI AGCT 5 cut(s) 220, 527, 578, 848, 876
AluI AGCT 5 cut(s) 220, 527, 578, 848, 876
Alw21I GWGCWC 1 cut(s) 222
AlwI GGATC 1 cut(s) 725
AoxI GGCC 6 cut(s) 51, 81, 243, 339, 365, 694
ApaI GGGCCC 2 cut(s) 85, 369
ApeKI GCWGC 3 cut(s) 153, 524, 578
ApoI RAATTY 1 cut(s) 899
Asp700I GAANNNNTTC 1 cut(s) 113
AspLEI GCGC 3 cut(s) 128, 265, 346
AspS9I GGNCC 7 cut(s) 81, 82, 339, 365, 366, 452, 694
AsuC2I CCSGG 3 cut(s) 50, 75, 333
AsuHPI GGTGA 1 cut(s) 16
AvaII GGWCC 1 cut(s) 452
BaeGI GKGCMC 2 cut(s) 85, 369
BanI GGYRCC 1 cut(s) 125
BanII GRGCYC 3 cut(s) 85, 222, 369
BbrPI CACGTG 1 cut(s) 211
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 165, 536, 565
BccI CCATC 3 cut(s) 50, 330, 705
BceAI ACGGC 5 cut(s) 113, 139, 230, 240, 319
BcgI CGANNNNNNTGC 2 cut(s) 142, 176
BciT130I CCWGG 2 cut(s) 370, 831
BclI TGATCA 1 cut(s) 856
BcnI CCSGG 3 cut(s) 50, 75, 333
BfaI CTAG 2 cut(s) 845, 905
BfmI CTRYAG 1 cut(s) 98
BfoI RGCGCY 1 cut(s) 129
BisI GCNGC 6 cut(s) 154, 317, 342, 347, 525, 579
BlsI GCNGC 6 cut(s) 155, 318, 343, 348, 526, 580
Bme1390I CCNGG 5 cut(s) 50, 75, 333, 370, 831
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 7 cut(s) 81, 82, 339, 365, 366, 452, 694
BmiI GGNNCC 6 cut(s) 83, 84, 127, 288, 367, 414
BmrFI CCNGG 5 cut(s) 50, 75, 333, 370, 831
BmsI GCATC 2 cut(s) 165, 511
BplI GAGNNNNNCTC 2 cut(s) 379, 411
BpmI CTGGAG 2 cut(s) 208, 503
Bpu10I CCTNAGC 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 250, 381
BpuMI CCSGG 3 cut(s) 50, 75, 333
BsaAI YACGTR 1 cut(s) 211
BsaHI GRCGYC 2 cut(s) 23, 126
BsaJI CCNNGG 3 cut(s) 356, 368, 829
BsaXI ACNNNNNCTCC 4 cut(s) 23, 53, 567, 597
Bsc4I CCNNNNNNNGG 3 cut(s) 323, 362, 375
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 2 cut(s) 596, 739
BseBI CCWGG 2 cut(s) 370, 831
BseDI CCNNGG 3 cut(s) 356, 368, 829
BseGI GGATG 3 cut(s) 274, 341, 526
BseLI CCNNNNNNNGG 3 cut(s) 323, 362, 375
BseMII CTCAG 1 cut(s) 408
BseNI ACTGG 2 cut(s) 596, 739
BseRI GAGGAG 1 cut(s) 923
BseSI GKGCMC 2 cut(s) 85, 369
BseXI GCAGC 3 cut(s) 165, 536, 565
BseYI CCCAGC 1 cut(s) 78
BsgI GTGCAG 1 cut(s) 128
Bsh1236I CGCG 2 cut(s) 344, 358
Bsh1285I CGRYCG 2 cut(s) 30, 282
BshFI GGCC 6 cut(s) 53, 83, 245, 341, 367, 696
BshNI GGYRCC 1 cut(s) 125
BsiEI CGRYCG 2 cut(s) 30, 282
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 4 cut(s) 50, 75, 201, 332
BslI CCNNNNNNNGG 3 cut(s) 323, 362, 375
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 6 cut(s) 53, 83, 245, 341, 367, 696
Bsp120I GGGCCC 2 cut(s) 81, 365
Bsp1286I GDGCHC 3 cut(s) 85, 222, 369
Bsp143I GATC 5 cut(s) 279, 400, 456, 730, 856
BspACI CCGC 7 cut(s) 36, 317, 324, 342, 347, 356, 358
BspANI GGCC 6 cut(s) 53, 83, 245, 341, 367, 696
BspCNI CTCAG 1 cut(s) 409
BspFNI CGCG 2 cut(s) 344, 358
BspLI GGNNCC 6 cut(s) 83, 84, 127, 288, 367, 414
BspPI GGATC 1 cut(s) 725
BspT107I GGYRCC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 2 cut(s) 596, 739
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 356, 368, 829
BssMI GATC 5 cut(s) 279, 400, 456, 730, 856
BssNI GRCGYC 2 cut(s) 23, 126
Bst2UI CCWGG 2 cut(s) 370, 831
Bst4CI ACNGT 2 cut(s) 271, 925
Bst6I CTCTTC 3 cut(s) 159, 380, 506
BstACI GRCGYC 2 cut(s) 23, 126
BstBAI YACGTR 1 cut(s) 211
BstC8I GCNNGC 4 cut(s) 184, 236, 471, 878
BstDEI CTNAG 2 cut(s) 374, 417
BstDSI CCRYGG 1 cut(s) 356
BstENI CCTNNNNNAGG 1 cut(s) 373
BstF5I GGATG 3 cut(s) 274, 341, 526
BstFNI CGCG 2 cut(s) 344, 358
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 3 cut(s) 128, 265, 346
BstKTI GATC 5 cut(s) 282, 403, 459, 733, 859
BstMBI GATC 5 cut(s) 279, 400, 456, 730, 856
BstMCI CGRYCG 2 cut(s) 30, 282
BstMWI GCNNNNNNNGC 3 cut(s) 125, 355, 364
BstNI CCWGG 2 cut(s) 370, 831
BstSCI CCNGG 5 cut(s) 48, 73, 331, 368, 829
BstSFI CTRYAG 1 cut(s) 98
BstSLI GKGCMC 2 cut(s) 85, 369
BstUI CGCG 2 cut(s) 344, 358
BstV1I GCAGC 3 cut(s) 165, 536, 565
BstX2I RGATCY 1 cut(s) 730
BstYI RGATCY 1 cut(s) 730
BsuRI GGCC 6 cut(s) 53, 83, 245, 341, 367, 696
BtgI CCRYGG 1 cut(s) 356
BtsCI GGATG 3 cut(s) 274, 341, 526
BtsIMutI CAGTG 1 cut(s) 459
Cac8I GCNNGC 4 cut(s) 184, 236, 471, 878
CfoI GCGC 3 cut(s) 128, 265, 346
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 7 cut(s) 81, 82, 339, 365, 366, 452, 694
Cfr42I CCGCGG 1 cut(s) 359
CseI GACGC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 495
CviAII CATG 3 cut(s) 106, 404, 449
CviQI GTAC 1 cut(s) 495
DdeI CTNAG 2 cut(s) 374, 417
DinI GGCGCC 1 cut(s) 127
DpnI GATC 5 cut(s) 281, 402, 458, 732, 858
DpnII GATC 5 cut(s) 279, 400, 456, 730, 856
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 3 cut(s) 159, 380, 506
EarI CTCTTC 3 cut(s) 159, 380, 506
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 3 cut(s) 85, 222, 369
Eco47I GGWCC 1 cut(s) 452
Eco53kI GAGCTC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 549
Eco72I CACGTG 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 220
EcoNI CCTNNNNNAGG 1 cut(s) 373
EcoO109I RGGNCCY 2 cut(s) 82, 366
EcoRII CCWGG 2 cut(s) 368, 829
EcoT38I GRGCYC 3 cut(s) 85, 222, 369
EgeI GGCGCC 1 cut(s) 127
EheI GGCGCC 1 cut(s) 127
FaeI CATG 3 cut(s) 109, 407, 452
FalI AAGNNNNNCTT 2 cut(s) 609, 641
FatI CATG 3 cut(s) 105, 403, 448
FauI CCCGC 2 cut(s) 43, 317
FauNDI CATATG 1 cut(s) 660
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 6 cut(s) 154, 317, 342, 347, 525, 579
FokI GGATG 3 cut(s) 261, 348, 533
FriOI GRGCYC 3 cut(s) 85, 222, 369
Fsp4HI GCNGC 6 cut(s) 154, 317, 342, 347, 525, 579
FspBI CTAG 2 cut(s) 845, 905
GlaI GCGC 3 cut(s) 127, 264, 345
GluI GCNGC 6 cut(s) 154, 317, 342, 347, 525, 579
GsaI CCCAGC 1 cut(s) 82
GsuI CTGGAG 2 cut(s) 208, 503
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 6 cut(s) 53, 83, 245, 341, 367, 696
HapII CCGG 4 cut(s) 50, 75, 201, 332
HgaI GACGC 1 cut(s) 188
HhaI GCGC 3 cut(s) 128, 265, 346
Hin1I GRCGYC 2 cut(s) 23, 126
Hin1II CATG 3 cut(s) 109, 407, 452
Hin6I GCGC 3 cut(s) 126, 263, 344
HinP1I GCGC 3 cut(s) 126, 263, 344
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 2 cut(s) 7, 352
HpaII CCGG 4 cut(s) 50, 75, 201, 332
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 3 cut(s) 27, 214, 641
Hpy188I TCNGA 4 cut(s) 12, 279, 675, 947
Hpy188III TCNNGA 5 cut(s) 229, 398, 416, 482, 854
Hpy8I GTNNAC 3 cut(s) 27, 214, 641
Hpy99I CGWCG 3 cut(s) 25, 28, 311
HpyAV CCTTC 2 cut(s) 102, 548
HpyCH4III ACNGT 2 cut(s) 271, 925
HpyCH4IV ACGT 4 cut(s) 23, 210, 309, 934
HpyCH4V TGCA 3 cut(s) 145, 524, 650
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 355, 364
HpyF3I CTNAG 2 cut(s) 374, 417
HpySE526I ACGT 4 cut(s) 23, 210, 309, 934
Hsp92I GRCGYC 2 cut(s) 23, 126
Hsp92II CATG 3 cut(s) 109, 407, 452
HspAI GCGC 3 cut(s) 126, 263, 344
KasI GGCGCC 1 cut(s) 125
Ksp22I TGATCA 1 cut(s) 856
KspI CCGCGG 1 cut(s) 359
Kzo9I GATC 5 cut(s) 279, 400, 456, 730, 856
LmnI GCTCC 2 cut(s) 575, 910
Lsp1109I GCAGC 3 cut(s) 165, 536, 565
LweI GCATC 2 cut(s) 165, 511
MaeI CTAG 2 cut(s) 845, 905
MaeII ACGT 4 cut(s) 23, 210, 309, 934
MaeIII GTNAC 2 cut(s) 427, 811
MalI GATC 5 cut(s) 281, 402, 458, 732, 858
MboI GATC 5 cut(s) 279, 400, 456, 730, 856
MboII GAAGA 7 cut(s) 176, 217, 397, 523, 614, 740, 934
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 3 cut(s) 85, 222, 369
MluCI AATT 1 cut(s) 899
Mly113I GGCGCC 1 cut(s) 126
MmeI TCCRAC 1 cut(s) 565
MroXI GAANNNNTTC 1 cut(s) 113
MspA1I CMGCKG 2 cut(s) 358, 527
MspI CCGG 4 cut(s) 50, 75, 201, 332
MspR9I CCNGG 5 cut(s) 50, 75, 333, 370, 831
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 2 cut(s) 370, 831
MvnI CGCG 2 cut(s) 344, 358
MwoI GCNNNNNNNGC 3 cut(s) 125, 355, 364
NarI GGCGCC 1 cut(s) 126
NciI CCSGG 3 cut(s) 50, 75, 333
NdeI CATATG 1 cut(s) 660
NdeII GATC 5 cut(s) 279, 400, 456, 730, 856
NlaIII CATG 3 cut(s) 109, 407, 452
NlaIV GGNNCC 6 cut(s) 83, 84, 127, 288, 367, 414
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 1 cut(s) 892
PdmI GAANNNNTTC 1 cut(s) 113
PfeI GAWTC 2 cut(s) 7, 352
PkrI GCNGC 6 cut(s) 155, 318, 343, 348, 526, 580
Ple19I CGATCG 1 cut(s) 282
PluTI GGCGCC 1 cut(s) 129
PmaCI CACGTG 1 cut(s) 211
PmlI CACGTG 1 cut(s) 211
Ppu21I YACGTR 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 2 cut(s) 368, 829
PspCI CACGTG 1 cut(s) 211
PspFI CCCAGC 1 cut(s) 78
PspGI CCWGG 2 cut(s) 368, 829
PspN4I GGNNCC 6 cut(s) 83, 84, 127, 288, 367, 414
PspOMI GGGCCC 2 cut(s) 81, 365
PspPI GGNCC 7 cut(s) 81, 82, 339, 365, 366, 452, 694
PsuI RGATCY 1 cut(s) 730
PvuI CGATCG 1 cut(s) 282
PvuII CAGCTG 1 cut(s) 527
RsaI GTAC 1 cut(s) 496
RsaNI GTAC 1 cut(s) 495
SacI GAGCTC 1 cut(s) 222
SacII CCGCGG 1 cut(s) 359
SalI GTCGAC 1 cut(s) 25
SatI GCNGC 6 cut(s) 154, 317, 342, 347, 525, 579
Sau3AI GATC 5 cut(s) 279, 400, 456, 730, 856
Sau96I GGNCC 7 cut(s) 81, 82, 339, 365, 366, 452, 694
ScrFI CCNGG 5 cut(s) 50, 75, 333, 370, 831
SduI GDGCHC 3 cut(s) 85, 222, 369
SfaNI GCATC 2 cut(s) 165, 511
SfcI CTRYAG 1 cut(s) 98
SfoI GGCGCC 1 cut(s) 127
Sfr303I CCGCGG 1 cut(s) 359
SgrAI CRCCGGYG 1 cut(s) 200
SgrBI CCGCGG 1 cut(s) 359
SinI GGWCC 1 cut(s) 452
SmlI CTYRAG 2 cut(s) 229, 396
SmoI CTYRAG 2 cut(s) 229, 396
Sse9I AATT 1 cut(s) 899
SsiI CCGC 7 cut(s) 36, 317, 324, 342, 347, 356, 358
SspDI GGCGCC 1 cut(s) 125
SspI AATATT 1 cut(s) 919
SspMI CTAG 2 cut(s) 845, 905
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 5 cut(s) 48, 73, 331, 368, 829
TaaI ACNGT 2 cut(s) 271, 925
TaiI ACGT 4 cut(s) 26, 213, 312, 937
TaqI TCGA 2 cut(s) 26, 162
TaqII GACCGA 2 cut(s) 44, 469
TasI AATT 1 cut(s) 899
TatI WGTACW 1 cut(s) 494
TauI GCSGC 3 cut(s) 319, 344, 349
TfiI GAWTC 2 cut(s) 7, 352
TscAI CASTG 1 cut(s) 466
TseI GCWGC 3 cut(s) 153, 524, 578
TspDTI ATGAA 2 cut(s) 122, 263
TspRI CASTG 1 cut(s) 466
VpaK11BI GGWCC 1 cut(s) 452
XagI CCTNNNNNAGG 1 cut(s) 373
XapI RAATTY 1 cut(s) 899
XmiI GTMKAC 1 cut(s) 26
XmnI GAANNNNTTC 1 cut(s) 113
XspI CTAG 2 cut(s) 845, 905
ZraI GACGTC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.