RchiOBHm_Chr4g0437351

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0008523
Physical Location & Seq
Reverse (-)
60479131 .. 60482692
3562 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40561

Sequence Viewer

Length: 1053 bp
ATGCTAGCCAGAACGACGCCGTCTCCACTGCTCACTTTTGCCACTACAAAACTGGCGTGCTGCTTCCACAGTTTGGCACCAAAAAAATCACCGAACAAGATGGTGAAGCTGATCGTGGCAACGACGACCGATCCGGCCTCCGTCAACCCTGCCAATGCTCTCTTATCCAAGCCCGGCTGGTCCCCAACCCCTTCTCTTCTACAGGAGGATATGAAGAGCTTCGCTAACGGAGCCGTTAGGGTTTTGGTGCACAACAGGAGCATAATCGAAGAGGACGACTTGGACGCTCGCTGGGAACAAGCCACCGGCGAACACGTGGACGAGCTCATCTTCTTGAGCAAGCACAAAGCCGCTTCCAGCCGCCCTGCGCTTACTGTTCACCCCATCGGGGTTCCTCATTTGCGAAACGGCGACGTTTTGCCGCAGGGCGGGAAGCCCGGCTGGGCCGCGCCGCCGAATCCTAGGATTGGGCCCTGGCTCAGGCTCTTGAAGAGAATCGCGCAAGATCGCAACTTGGTCCCTGAGTTCGAGGTGACTTTGGAGGCTACACATCATGGACCGATTACAGACACGCCTGCCATGTTCCTGGAGATTGGCAGTACAGATGATTGCTGGAAGAGGCAGGATGCAGCTGAAGTTATGGCTCTATTAGTTTGGGAAGGACTTGGGCTTGGAGGGGGAGCTGCTGTTGGAAACTGGAACGGGGGGAACGATAATAAAAGAGTTCTGCTTGGGATAGGTGGTGGACATTATGTACCTCGTCATATGGATATAGCTTTGAAAGATGATGTCTGGGTGGGCCATCTAATTTCGGGTTATTCGTTACCAATGGAAGATCCGAGCCAGTCCAAGACAGAAACAAATACAAAAGATATCGGTGGAACTTGGAAACAATCAATCAAAGTAGCATTTGAGGCTACGCAATCGGCTTTCCCTGGTGGAGAAATTGTAGCTCATCTTGATCACAAGAGTTTCAAAAGCTGGCAAAAGAATGCCATAACGAGTTTCCTAGAGGAGCAGAACATTACAATTAGCAAACGCGATTTCTTCTGA

Protein Analysis

350

Amino Acids

38.19

Weight (kDa)

6.67

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 103 - 344 2.5e-58 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AccB7I CCANNNNNTGG 1 cut(s) 73
AccII CGCG 3 cut(s) 449, 500, 1041
AciI CCGC 6 cut(s) 351, 361, 422, 429, 447, 452
AclWI GGATC 2 cut(s) 125, 830
AcuI CTGAAG 1 cut(s) 654
AcvI CACGTG 1 cut(s) 316
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 2 cut(s) 601, 756
AfiI CCNNNNNNNGG 5 cut(s) 73, 388, 428, 467, 480
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 3 cut(s) 490, 781, 976
AjnI CCWGG 3 cut(s) 473, 585, 934
AluBI AGCT 8 cut(s) 109, 219, 325, 632, 683, 776, 953, 981
AluI AGCT 8 cut(s) 109, 219, 325, 632, 683, 776, 953, 981
Alw21I GWGCWC 2 cut(s) 252, 327
Alw26I GTCTC 1 cut(s) 27
Alw44I GTGCAC 1 cut(s) 248
AlwI GGATC 2 cut(s) 125, 830
AoxI GGCC 4 cut(s) 135, 444, 470, 799
ApaI GGGCCC 1 cut(s) 474
ApaLI GTGCAC 1 cut(s) 248
ApeKI GCWGC 3 cut(s) 60, 629, 683
Asp700I GAANNNNTTC 1 cut(s) 218
AspA2I CCTAGG 1 cut(s) 461
AspLEI GCGC 3 cut(s) 370, 451, 502
AspS9I GGNCC 7 cut(s) 180, 444, 470, 471, 517, 557, 799
AsuC2I CCSGG 2 cut(s) 174, 438
AsuHPI GGTGA 4 cut(s) 81, 115, 371, 544
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 3 cut(s) 180, 517, 557
AvrII CCTAGG 1 cut(s) 461
BaeGI GKGCMC 2 cut(s) 252, 474
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 2 cut(s) 327, 474
BbrPI CACGTG 1 cut(s) 316
Bbv12I GWGCWC 2 cut(s) 252, 327
BbvI GCAGC 3 cut(s) 47, 641, 670
BccI CCATC 3 cut(s) 94, 392, 810
BceAI ACGGC 3 cut(s) 4, 218, 424
BciT130I CCWGG 3 cut(s) 475, 587, 936
BclI TGATCA 1 cut(s) 961
BcnI CCSGG 2 cut(s) 174, 438
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 3 cut(s) 5, 462, 1010
BfmI CTRYAG 1 cut(s) 200
BisI GCNGC 8 cut(s) 61, 351, 361, 422, 447, 452, 630, 684
BlnI CCTAGG 1 cut(s) 461
BlsI GCNGC 8 cut(s) 62, 352, 362, 423, 448, 453, 631, 685
Bme1390I CCNGG 5 cut(s) 174, 438, 475, 587, 936
Bme18I GGWCC 3 cut(s) 180, 517, 557
BmgT120I GGNCC 7 cut(s) 180, 444, 470, 471, 517, 557, 799
BmiI GGNNCC 6 cut(s) 78, 182, 232, 393, 472, 519
BmrFI CCNGG 5 cut(s) 174, 438, 475, 587, 936
BmsI GCATC 1 cut(s) 616
BmtI GCTAGC 1 cut(s) 8
BpmI CTGGAG 1 cut(s) 608
Bpu10I CCTNAGC 1 cut(s) 479
BpuEI CTTGAG 1 cut(s) 355
BpuMI CCSGG 2 cut(s) 174, 438
BsaAI YACGTR 1 cut(s) 316
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 461, 473, 934
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 672, 702, 933, 963
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 388, 428, 467, 480
Bse118I RCCGGY 1 cut(s) 305
Bse1I ACTGG 3 cut(s) 57, 701, 844
BseBI CCWGG 3 cut(s) 475, 587, 936
BseDI CCNNGG 3 cut(s) 461, 473, 934
BseGI GGATG 1 cut(s) 631
BseLI CCNNNNNNNGG 5 cut(s) 73, 388, 428, 467, 480
BseMII CTCAG 2 cut(s) 493, 513
BseNI ACTGG 3 cut(s) 57, 701, 844
BseRI GAGGAG 1 cut(s) 1028
BseSI GKGCMC 2 cut(s) 252, 474
BseXI GCAGC 3 cut(s) 47, 641, 670
BseYI CCCAGC 2 cut(s) 291, 441
Bsh1236I CGCG 3 cut(s) 449, 500, 1041
Bsh1285I CGRYCG 1 cut(s) 129
BshFI GGCC 4 cut(s) 137, 446, 472, 801
BshNI GGYRCC 1 cut(s) 76
BsiEI CGRYCG 1 cut(s) 129
BsiHKAI GWGCWC 2 cut(s) 252, 327
BsiSI CCGG 4 cut(s) 134, 174, 306, 438
BslFI GGGAC 2 cut(s) 166, 503
BslI CCNNNNNNNGG 5 cut(s) 73, 388, 428, 467, 480
BsmAI GTCTC 1 cut(s) 27
BsmBI CGTCTC 1 cut(s) 27
BsmFI GGGAC 2 cut(s) 166, 503
BsmI GAATGC 1 cut(s) 997
BsnI GGCC 4 cut(s) 137, 446, 472, 801
Bsp120I GGGCCC 1 cut(s) 470
Bsp1286I GDGCHC 3 cut(s) 252, 327, 474
Bsp143I GATC 5 cut(s) 111, 130, 505, 835, 961
BspACI CCGC 6 cut(s) 351, 361, 422, 429, 447, 452
BspANI GGCC 4 cut(s) 137, 446, 472, 801
BspCNI CTCAG 2 cut(s) 492, 514
BspFNI CGCG 3 cut(s) 449, 500, 1041
BspLI GGNNCC 6 cut(s) 78, 182, 232, 393, 472, 519
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 125, 830
BspQI GCTCTTC 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 305
BsrI ACTGG 3 cut(s) 57, 701, 844
BssAI RCCGGY 1 cut(s) 305
BssECI CCNNGG 3 cut(s) 461, 473, 934
BssMI GATC 5 cut(s) 111, 130, 505, 835, 961
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 461
Bst2UI CCWGG 3 cut(s) 475, 587, 936
Bst4CI ACNGT 2 cut(s) 71, 376
Bst6I CTCTTC 5 cut(s) 201, 209, 264, 485, 611
BstACI GRCGYC 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 316
BstC8I GCNNGC 6 cut(s) 6, 58, 289, 341, 576, 983
BstDEI CTNAG 2 cut(s) 479, 522
BstENI CCTNNNNNAGG 1 cut(s) 478
BstF5I GGATG 1 cut(s) 631
BstFNI CGCG 3 cut(s) 449, 500, 1041
BstHHI GCGC 3 cut(s) 370, 451, 502
BstKTI GATC 5 cut(s) 114, 133, 508, 838, 964
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 5 cut(s) 111, 130, 505, 835, 961
BstMCI CGRYCG 1 cut(s) 129
BstMWI GCNNNNNNNGC 2 cut(s) 230, 914
BstNI CCWGG 3 cut(s) 475, 587, 936
BstSCI CCNGG 5 cut(s) 172, 436, 473, 585, 934
BstSFI CTRYAG 1 cut(s) 200
BstSLI GKGCMC 2 cut(s) 252, 474
BstUI CGCG 3 cut(s) 449, 500, 1041
BstV1I GCAGC 3 cut(s) 47, 641, 670
BstX2I RGATCY 1 cut(s) 835
BstXI CCANNNNNNTGG 1 cut(s) 586
BstYI RGATCY 1 cut(s) 835
BsuRI GGCC 4 cut(s) 137, 446, 472, 801
BtsCI GGATG 1 cut(s) 631
BtsI GCAGTG 1 cut(s) 26
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 6 cut(s) 6, 58, 289, 341, 576, 983
CfoI GCGC 3 cut(s) 370, 451, 502
Cfr10I RCCGGY 1 cut(s) 305
Cfr13I GGNCC 7 cut(s) 180, 444, 470, 471, 517, 557, 799
CseI GACGC 2 cut(s) 25, 293
Csp6I GTAC 2 cut(s) 600, 755
CviAII CATG 2 cut(s) 554, 580
CviQI GTAC 2 cut(s) 600, 755
DdeI CTNAG 2 cut(s) 479, 522
DpnI GATC 5 cut(s) 113, 132, 507, 837, 963
DpnII GATC 5 cut(s) 111, 130, 505, 835, 961
Eam1104I CTCTTC 5 cut(s) 201, 209, 264, 485, 611
EarI CTCTTC 5 cut(s) 201, 209, 264, 485, 611
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 461
Eco24I GRGCYC 2 cut(s) 327, 474
Eco32I GATATC 1 cut(s) 874
Eco47I GGWCC 3 cut(s) 180, 517, 557
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 1 cut(s) 654
Eco72I CACGTG 1 cut(s) 316
EcoICRI GAGCTC 1 cut(s) 325
EcoNI CCTNNNNNAGG 1 cut(s) 478
EcoO109I RGGNCCY 1 cut(s) 471
EcoRII CCWGG 3 cut(s) 473, 585, 934
EcoRV GATATC 1 cut(s) 874
EcoT14I CCWWGG 1 cut(s) 461
EcoT38I GRGCYC 2 cut(s) 327, 474
ErhI CCWWGG 1 cut(s) 461
Esp3I CGTCTC 1 cut(s) 27
FaeI CATG 2 cut(s) 557, 583
FaqI GGGAC 2 cut(s) 166, 503
FatI CATG 2 cut(s) 553, 579
FauI CCCGC 1 cut(s) 422
FauNDI CATATG 1 cut(s) 765
FbaI TGATCA 1 cut(s) 961
Fnu4HI GCNGC 8 cut(s) 61, 351, 361, 422, 447, 452, 630, 684
FokI GGATG 1 cut(s) 638
FriOI GRGCYC 2 cut(s) 327, 474
Fsp4HI GCNGC 8 cut(s) 61, 351, 361, 422, 447, 452, 630, 684
FspBI CTAG 3 cut(s) 5, 462, 1010
GlaI GCGC 3 cut(s) 369, 450, 501
GluI GCNGC 8 cut(s) 61, 351, 361, 422, 447, 452, 630, 684
GsaI CCCAGC 2 cut(s) 295, 445
GsuI CTGGAG 1 cut(s) 608
HaeIII GGCC 4 cut(s) 137, 446, 472, 801
HapII CCGG 4 cut(s) 134, 174, 306, 438
HgaI GACGC 2 cut(s) 25, 293
HhaI GCGC 3 cut(s) 370, 451, 502
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 2 cut(s) 557, 583
Hin6I GCGC 3 cut(s) 368, 449, 500
HinP1I GCGC 3 cut(s) 368, 449, 500
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HinfI GANTC 2 cut(s) 457, 495
HpaII CCGG 4 cut(s) 134, 174, 306, 438
HphI GGTGA 4 cut(s) 81, 115, 371, 544
Hpy166II GTNNAC 5 cut(s) 145, 250, 319, 379, 746
Hpy188I TCNGA 2 cut(s) 840, 1052
Hpy188III TCNNGA 3 cut(s) 334, 487, 959
Hpy8I GTNNAC 5 cut(s) 145, 250, 319, 379, 746
Hpy99I CGWCG 3 cut(s) 19, 127, 416
HpyAV CCTTC 2 cut(s) 201, 653
HpyCH4III ACNGT 2 cut(s) 71, 376
HpyCH4IV ACGT 2 cut(s) 315, 414
HpyCH4V TGCA 2 cut(s) 250, 629
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 914
HpyF3I CTNAG 2 cut(s) 479, 522
HpySE526I ACGT 2 cut(s) 315, 414
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 2 cut(s) 557, 583
HspAI GCGC 3 cut(s) 368, 449, 500
Ksp22I TGATCA 1 cut(s) 961
Kzo9I GATC 5 cut(s) 111, 130, 505, 835, 961
LguI GCTCTTC 1 cut(s) 209
LmnI GCTCC 4 cut(s) 230, 258, 680, 1015
Lsp1109I GCAGC 3 cut(s) 47, 641, 670
LweI GCATC 1 cut(s) 616
MaeI CTAG 3 cut(s) 5, 462, 1010
MaeII ACGT 2 cut(s) 315, 414
MaeIII GTNAC 2 cut(s) 532, 822
MalI GATC 5 cut(s) 113, 132, 507, 837, 963
MboI GATC 5 cut(s) 111, 130, 505, 835, 961
MboII GAAGA 8 cut(s) 188, 226, 281, 322, 502, 628, 845, 1039
MflI RGATCY 1 cut(s) 835
MhlI GDGCHC 3 cut(s) 252, 327, 474
MluCI AATT 3 cut(s) 807, 945, 1029
MmeI TCCRAC 1 cut(s) 670
MroXI GAANNNNTTC 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 632
MspI CCGG 4 cut(s) 134, 174, 306, 438
MspR9I CCNGG 5 cut(s) 174, 438, 475, 587, 936
Mva1269I GAATGC 1 cut(s) 997
MvaI CCWGG 3 cut(s) 475, 587, 936
MvnI CGCG 3 cut(s) 449, 500, 1041
MwoI GCNNNNNNNGC 2 cut(s) 230, 914
NciI CCSGG 2 cut(s) 174, 438
NdeI CATATG 1 cut(s) 765
NdeII GATC 5 cut(s) 111, 130, 505, 835, 961
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 2 cut(s) 557, 583
NlaIV GGNNCC 6 cut(s) 78, 182, 232, 393, 472, 519
NmuCI GTSAC 1 cut(s) 532
PciSI GCTCTTC 1 cut(s) 209
PcsI WCGNNNNNNNCGW 3 cut(s) 273, 708, 818
PctI GAATGC 1 cut(s) 997
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 2 cut(s) 457, 495
PflFI GACNNNGTC 1 cut(s) 19
PflMI CCANNNNNTGG 1 cut(s) 73
PfoI TCCNGGA 1 cut(s) 585
PkrI GCNGC 8 cut(s) 62, 352, 362, 423, 448, 453, 631, 685
PmaCI CACGTG 1 cut(s) 316
PmlI CACGTG 1 cut(s) 316
Ppu21I YACGTR 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 3 cut(s) 473, 585, 934
PspCI CACGTG 1 cut(s) 316
PspFI CCCAGC 2 cut(s) 291, 441
PspGI CCWGG 3 cut(s) 473, 585, 934
PspN4I GGNNCC 6 cut(s) 78, 182, 232, 393, 472, 519
PspOMI GGGCCC 1 cut(s) 470
PspPI GGNCC 7 cut(s) 180, 444, 470, 471, 517, 557, 799
PsuI RGATCY 1 cut(s) 835
PsyI GACNNNGTC 1 cut(s) 19
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 2 cut(s) 601, 756
RsaNI GTAC 2 cut(s) 600, 755
SacI GAGCTC 1 cut(s) 327
SapI GCTCTTC 1 cut(s) 209
SatI GCNGC 8 cut(s) 61, 351, 361, 422, 447, 452, 630, 684
Sau3AI GATC 5 cut(s) 111, 130, 505, 835, 961
Sau96I GGNCC 7 cut(s) 180, 444, 470, 471, 517, 557, 799
ScrFI CCNGG 5 cut(s) 174, 438, 475, 587, 936
SduI GDGCHC 3 cut(s) 252, 327, 474
SfaNI GCATC 1 cut(s) 616
SfcI CTRYAG 1 cut(s) 200
SgrAI CRCCGGYG 1 cut(s) 305
SinI GGWCC 3 cut(s) 180, 517, 557
SmlI CTYRAG 1 cut(s) 334
SmoI CTYRAG 1 cut(s) 334
Sse9I AATT 3 cut(s) 807, 945, 1029
SsiI CCGC 6 cut(s) 351, 361, 422, 429, 447, 452
SspMI CTAG 3 cut(s) 5, 462, 1010
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 5 cut(s) 172, 436, 473, 585, 934
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 2 cut(s) 71, 376
TaiI ACGT 2 cut(s) 318, 417
TaqI TCGA 2 cut(s) 267, 528
TaqII GACCGA 2 cut(s) 143, 574
TasI AATT 3 cut(s) 807, 945, 1029
TatI WGTACW 1 cut(s) 599
TauI GCSGC 5 cut(s) 353, 363, 424, 449, 454
TfiI GAWTC 2 cut(s) 457, 495
TscAI CASTG 1 cut(s) 33
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 3 cut(s) 60, 629, 683
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 1 cut(s) 227
TspGWI ACGGA 2 cut(s) 130, 243
TspRI CASTG 1 cut(s) 33
Tth111I GACNNNGTC 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 73
VneI GTGCAC 1 cut(s) 248
VpaK11BI GGWCC 3 cut(s) 180, 517, 557
XagI CCTNNNNNAGG 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XmaJI CCTAGG 1 cut(s) 461
XmnI GAANNNNTTC 1 cut(s) 218
XspI CTAG 3 cut(s) 5, 462, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.