Rh4DG358800

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008523
Physical Location & Seq
Reverse (-)
57938480 .. 57942168
3689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG358800.1

Sequence Viewer

Length: 954 bp
ATGGTGAAGCTGATCGTGGCAACGACGACCGATCCGGCCTCCGTCAACCCTGCCAATGCTCTCTTATCCAAGCCCGGCTGGTCCCCAACCCCTTCTCTTCTACAGGAGGATATGAAGAGCTTCGCTAACGGAGCCGTTAGGGTTTTGATGCACAACAGGAGCATAATCGAAGAGGACGACTTGGACGCTCGCTGGGAACAAGCCACCGGCGAACACGTGGACGAGCTCATCTTCTTGAGCAAGCACAAAGCCGCTTCCAGCCGCCCTGCGCTTACTGTTCACCCCATCGGGGTTCCTCATTTGCGAAACGGCGACGTTTTGCCGCAGGGCGGGAAGCCCGGCTGGGCCGCGCCGCCGAATCCTAGGATTGGGCCCTGGCTCAGGCTCTTGAAGAGAATCGCGCAAGATCGCAACTTGGTCCCTGAGTTCGAGGTGACTTTGGAGGCTACACATCATGGACCGATTACAGACACGCCTGCCATGTTCCTGGAGATTGGCAGTACAGATGATTGCTGGAAGAGGCAGGATGCAGCTGAAGTTATGGCTCTATTAGTTTGGGAAGGACTTGGGCTTGGAGGGGGAGCTGCTGTTGGAAACTGGAACGGGGGGAACGATAATAAAAGAGTTCTGCTTGGGATAGGTGGTGGACATTATGTGCCTCGTCATATGGATATAGCTCTGAAAGATGATGTCTGGGTGGGCCATTTAATTTCGGGTTATTCGTTACCAATGGAAGATCCGAGCCAGTCCAAGACAGAAACAAATACAAAAGATATCGGTGGAACTTGGAAACAATCAATCAAAGTAGCATTTGAGGCTACGCAATCGGCTTTCCCTGGTGGAGAAATTGTAGCTCATCTTGATCACAAGAGTTTCAAAAGCTGGCAAAAGAATGCCATAACGAGTTTCCTAGAGGAGCAGAACATTACAATTAGCAAACGCGATTTCTTCTGA

Protein Analysis

317

Amino Acids

34.66

Weight (kDa)

5.94

Isoelectric Point (pI)

49.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 70 - 311 1.8e-58 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 350, 401, 942
AciI CCGC 6 cut(s) 252, 262, 323, 330, 348, 353
AclWI GGATC 2 cut(s) 26, 731
AcuI CTGAAG 1 cut(s) 555
AcvI CACGTG 1 cut(s) 217
AfaI GTAC 1 cut(s) 502
AfiI CCNNNNNNNGG 4 cut(s) 289, 329, 368, 381
AflIII ACRYGT 1 cut(s) 214
AgsI TTSAA 2 cut(s) 391, 877
AjnI CCWGG 3 cut(s) 374, 486, 835
AluBI AGCT 8 cut(s) 10, 120, 226, 533, 584, 677, 854, 882
AluI AGCT 8 cut(s) 10, 120, 226, 533, 584, 677, 854, 882
Alw21I GWGCWC 1 cut(s) 228
AlwI GGATC 2 cut(s) 26, 731
AoxI GGCC 4 cut(s) 36, 345, 371, 700
ApaI GGGCCC 1 cut(s) 375
ApeKI GCWGC 2 cut(s) 530, 584
Asp700I GAANNNNTTC 1 cut(s) 119
AspA2I CCTAGG 1 cut(s) 362
AspLEI GCGC 3 cut(s) 271, 352, 403
AspS9I GGNCC 7 cut(s) 81, 345, 371, 372, 418, 458, 700
AsuC2I CCSGG 2 cut(s) 75, 339
AsuHPI GGTGA 3 cut(s) 16, 272, 445
AvaII GGWCC 3 cut(s) 81, 418, 458
AvrII CCTAGG 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 375
BanII GRGCYC 2 cut(s) 228, 375
BbrPI CACGTG 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 542, 571
BccI CCATC 1 cut(s) 293
BceAI ACGGC 2 cut(s) 119, 325
BciT130I CCWGG 3 cut(s) 376, 488, 837
BclI TGATCA 1 cut(s) 862
BcnI CCSGG 2 cut(s) 75, 339
BfaI CTAG 2 cut(s) 363, 911
BfmI CTRYAG 1 cut(s) 101
BisI GCNGC 7 cut(s) 252, 262, 323, 348, 353, 531, 585
BlnI CCTAGG 1 cut(s) 362
BlsI GCNGC 7 cut(s) 253, 263, 324, 349, 354, 532, 586
Bme1390I CCNGG 5 cut(s) 75, 339, 376, 488, 837
Bme18I GGWCC 3 cut(s) 81, 418, 458
BmgT120I GGNCC 7 cut(s) 81, 345, 371, 372, 418, 458, 700
BmiI GGNNCC 5 cut(s) 83, 133, 294, 373, 420
BmrFI CCNGG 5 cut(s) 75, 339, 376, 488, 837
BmsI GCATC 2 cut(s) 138, 517
BpmI CTGGAG 1 cut(s) 509
Bpu10I CCTNAGC 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 256
BpuMI CCSGG 2 cut(s) 75, 339
BsaAI YACGTR 1 cut(s) 217
BsaJI CCNNGG 3 cut(s) 362, 374, 835
BsaXI ACNNNNNCTCC 4 cut(s) 573, 603, 834, 864
Bsc4I CCNNNNNNNGG 4 cut(s) 289, 329, 368, 381
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 2 cut(s) 602, 745
BseBI CCWGG 3 cut(s) 376, 488, 837
BseDI CCNNGG 3 cut(s) 362, 374, 835
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 4 cut(s) 289, 329, 368, 381
BseMII CTCAG 2 cut(s) 394, 414
BseNI ACTGG 2 cut(s) 602, 745
BseRI GAGGAG 1 cut(s) 929
BseSI GKGCMC 1 cut(s) 375
BseXI GCAGC 2 cut(s) 542, 571
BseYI CCCAGC 2 cut(s) 192, 342
Bsh1236I CGCG 3 cut(s) 350, 401, 942
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 4 cut(s) 38, 347, 373, 702
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 4 cut(s) 35, 75, 207, 339
BslFI GGGAC 2 cut(s) 67, 404
BslI CCNNNNNNNGG 4 cut(s) 289, 329, 368, 381
BsmFI GGGAC 2 cut(s) 67, 404
BsmI GAATGC 1 cut(s) 898
BsnI GGCC 4 cut(s) 38, 347, 373, 702
Bsp120I GGGCCC 1 cut(s) 371
Bsp1286I GDGCHC 2 cut(s) 228, 375
Bsp143I GATC 5 cut(s) 12, 31, 406, 736, 862
BspACI CCGC 6 cut(s) 252, 262, 323, 330, 348, 353
BspANI GGCC 4 cut(s) 38, 347, 373, 702
BspCNI CTCAG 2 cut(s) 393, 415
BspFNI CGCG 3 cut(s) 350, 401, 942
BspLI GGNNCC 5 cut(s) 83, 133, 294, 373, 420
BspPI GGATC 2 cut(s) 26, 731
BspQI GCTCTTC 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 2 cut(s) 602, 745
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 3 cut(s) 362, 374, 835
BssMI GATC 5 cut(s) 12, 31, 406, 736, 862
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 3 cut(s) 376, 488, 837
Bst4CI ACNGT 1 cut(s) 277
Bst6I CTCTTC 5 cut(s) 102, 110, 165, 386, 512
BstBAI YACGTR 1 cut(s) 217
BstC8I GCNNGC 4 cut(s) 190, 242, 477, 884
BstDEI CTNAG 2 cut(s) 380, 423
BstENI CCTNNNNNAGG 1 cut(s) 379
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 3 cut(s) 350, 401, 942
BstHHI GCGC 3 cut(s) 271, 352, 403
BstKTI GATC 5 cut(s) 15, 34, 409, 739, 865
BstMBI GATC 5 cut(s) 12, 31, 406, 736, 862
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 2 cut(s) 131, 815
BstNI CCWGG 3 cut(s) 376, 488, 837
BstSCI CCNGG 5 cut(s) 73, 337, 374, 486, 835
BstSFI CTRYAG 1 cut(s) 101
BstSLI GKGCMC 1 cut(s) 375
BstUI CGCG 3 cut(s) 350, 401, 942
BstV1I GCAGC 2 cut(s) 542, 571
BstX2I RGATCY 1 cut(s) 736
BstXI CCANNNNNNTGG 1 cut(s) 487
BstYI RGATCY 1 cut(s) 736
BsuRI GGCC 4 cut(s) 38, 347, 373, 702
BtsCI GGATG 1 cut(s) 532
Cac8I GCNNGC 4 cut(s) 190, 242, 477, 884
CfoI GCGC 3 cut(s) 271, 352, 403
Cfr10I RCCGGY 1 cut(s) 206
Cfr13I GGNCC 7 cut(s) 81, 345, 371, 372, 418, 458, 700
CseI GACGC 1 cut(s) 194
Csp6I GTAC 1 cut(s) 501
CviAII CATG 2 cut(s) 455, 481
CviQI GTAC 1 cut(s) 501
DdeI CTNAG 2 cut(s) 380, 423
DpnI GATC 5 cut(s) 14, 33, 408, 738, 864
DpnII GATC 5 cut(s) 12, 31, 406, 736, 862
Eam1104I CTCTTC 5 cut(s) 102, 110, 165, 386, 512
EarI CTCTTC 5 cut(s) 102, 110, 165, 386, 512
Ecl136II GAGCTC 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 362
Eco24I GRGCYC 2 cut(s) 228, 375
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 3 cut(s) 81, 418, 458
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 555
Eco72I CACGTG 1 cut(s) 217
EcoICRI GAGCTC 1 cut(s) 226
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 372
EcoRII CCWGG 3 cut(s) 374, 486, 835
EcoRV GATATC 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 362
EcoT38I GRGCYC 2 cut(s) 228, 375
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 2 cut(s) 458, 484
FaqI GGGAC 2 cut(s) 67, 404
FatI CATG 2 cut(s) 454, 480
FauI CCCGC 1 cut(s) 323
FauNDI CATATG 1 cut(s) 666
FbaI TGATCA 1 cut(s) 862
Fnu4HI GCNGC 7 cut(s) 252, 262, 323, 348, 353, 531, 585
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 2 cut(s) 228, 375
Fsp4HI GCNGC 7 cut(s) 252, 262, 323, 348, 353, 531, 585
FspBI CTAG 2 cut(s) 363, 911
GlaI GCGC 3 cut(s) 270, 351, 402
GluI GCNGC 7 cut(s) 252, 262, 323, 348, 353, 531, 585
GsaI CCCAGC 2 cut(s) 196, 346
GsuI CTGGAG 1 cut(s) 509
HaeIII GGCC 4 cut(s) 38, 347, 373, 702
HapII CCGG 4 cut(s) 35, 75, 207, 339
HgaI GACGC 1 cut(s) 194
HhaI GCGC 3 cut(s) 271, 352, 403
Hin1II CATG 2 cut(s) 458, 484
Hin6I GCGC 3 cut(s) 269, 350, 401
HinP1I GCGC 3 cut(s) 269, 350, 401
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 2 cut(s) 358, 396
HpaII CCGG 4 cut(s) 35, 75, 207, 339
HphI GGTGA 3 cut(s) 16, 272, 445
Hpy166II GTNNAC 4 cut(s) 46, 220, 280, 647
Hpy188I TCNGA 3 cut(s) 681, 741, 953
Hpy188III TCNNGA 3 cut(s) 235, 388, 860
Hpy8I GTNNAC 4 cut(s) 46, 220, 280, 647
Hpy99I CGWCG 2 cut(s) 28, 317
HpyAV CCTTC 2 cut(s) 102, 554
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 2 cut(s) 216, 315
HpyCH4V TGCA 2 cut(s) 151, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 815
HpyF3I CTNAG 2 cut(s) 380, 423
HpySE526I ACGT 2 cut(s) 216, 315
Hsp92II CATG 2 cut(s) 458, 484
HspAI GCGC 3 cut(s) 269, 350, 401
Ksp22I TGATCA 1 cut(s) 862
Kzo9I GATC 5 cut(s) 12, 31, 406, 736, 862
LguI GCTCTTC 1 cut(s) 110
LmnI GCTCC 4 cut(s) 131, 159, 581, 916
Lsp1109I GCAGC 2 cut(s) 542, 571
LweI GCATC 2 cut(s) 138, 517
MaeI CTAG 2 cut(s) 363, 911
MaeII ACGT 2 cut(s) 216, 315
MaeIII GTNAC 2 cut(s) 433, 723
MalI GATC 5 cut(s) 14, 33, 408, 738, 864
MboI GATC 5 cut(s) 12, 31, 406, 736, 862
MboII GAAGA 8 cut(s) 89, 127, 182, 223, 403, 529, 746, 940
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 2 cut(s) 228, 375
MluCI AATT 3 cut(s) 708, 846, 930
MmeI TCCRAC 1 cut(s) 571
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 1 cut(s) 707
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 4 cut(s) 35, 75, 207, 339
MspR9I CCNGG 5 cut(s) 75, 339, 376, 488, 837
Mva1269I GAATGC 1 cut(s) 898
MvaI CCWGG 3 cut(s) 376, 488, 837
MvnI CGCG 3 cut(s) 350, 401, 942
MwoI GCNNNNNNNGC 2 cut(s) 131, 815
NciI CCSGG 2 cut(s) 75, 339
NdeI CATATG 1 cut(s) 666
NdeII GATC 5 cut(s) 12, 31, 406, 736, 862
NlaIII CATG 2 cut(s) 458, 484
NlaIV GGNNCC 5 cut(s) 83, 133, 294, 373, 420
NmuCI GTSAC 1 cut(s) 433
PciSI GCTCTTC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 3 cut(s) 174, 609, 719
PctI GAATGC 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 358, 396
PfoI TCCNGGA 1 cut(s) 486
PkrI GCNGC 7 cut(s) 253, 263, 324, 349, 354, 532, 586
PmaCI CACGTG 1 cut(s) 217
PmlI CACGTG 1 cut(s) 217
Ppu21I YACGTR 1 cut(s) 217
Psp124BI GAGCTC 1 cut(s) 228
Psp6I CCWGG 3 cut(s) 374, 486, 835
PspCI CACGTG 1 cut(s) 217
PspFI CCCAGC 2 cut(s) 192, 342
PspGI CCWGG 3 cut(s) 374, 486, 835
PspN4I GGNNCC 5 cut(s) 83, 133, 294, 373, 420
PspOMI GGGCCC 1 cut(s) 371
PspPI GGNCC 7 cut(s) 81, 345, 371, 372, 418, 458, 700
PsuI RGATCY 1 cut(s) 736
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 1 cut(s) 502
RsaNI GTAC 1 cut(s) 501
SacI GAGCTC 1 cut(s) 228
SapI GCTCTTC 1 cut(s) 110
SaqAI TTAA 1 cut(s) 707
SatI GCNGC 7 cut(s) 252, 262, 323, 348, 353, 531, 585
Sau3AI GATC 5 cut(s) 12, 31, 406, 736, 862
Sau96I GGNCC 7 cut(s) 81, 345, 371, 372, 418, 458, 700
ScrFI CCNGG 5 cut(s) 75, 339, 376, 488, 837
SduI GDGCHC 2 cut(s) 228, 375
SfaNI GCATC 2 cut(s) 138, 517
SfcI CTRYAG 1 cut(s) 101
SgrAI CRCCGGYG 1 cut(s) 206
SinI GGWCC 3 cut(s) 81, 418, 458
SmlI CTYRAG 1 cut(s) 235
SmoI CTYRAG 1 cut(s) 235
Sse9I AATT 3 cut(s) 708, 846, 930
SsiI CCGC 6 cut(s) 252, 262, 323, 330, 348, 353
SspMI CTAG 2 cut(s) 363, 911
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 5 cut(s) 73, 337, 374, 486, 835
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 2 cut(s) 219, 318
TaqI TCGA 2 cut(s) 168, 429
TaqII GACCGA 2 cut(s) 44, 475
TasI AATT 3 cut(s) 708, 846, 930
TatI WGTACW 1 cut(s) 500
TauI GCSGC 5 cut(s) 254, 264, 325, 350, 355
TfiI GAWTC 2 cut(s) 358, 396
Tru1I TTAA 1 cut(s) 707
Tru9I TTAA 1 cut(s) 707
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 2 cut(s) 530, 584
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 1 cut(s) 128
TspGWI ACGGA 2 cut(s) 31, 144
VpaK11BI GGWCC 3 cut(s) 81, 418, 458
XagI CCTNNNNNAGG 1 cut(s) 379
XmaJI CCTAGG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 119
XspI CTAG 2 cut(s) 363, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.