MD13G1056600.v1.1

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0008523
Physical Location & Seq
Reverse (-)
3950034 .. 3953106
3073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1056600.v1.1.491

Sequence Viewer

Length: 969 bp
ATGGTGACGCTGATCGTGGCGACGACCACCGACCCGGCCTCTGTCAACCCGGCCAAGGCCCTCCTAGCCATGCCCGGCTGGGACCCCTCCCCTTCTTGTTTACAGGACATGAAGGGGTTTGGCAATGGAGGAGGAGGAGTCAGGGTTTTGCTGCACGGTAGGGGAATTGTGGAAGAAGACGACTTGGACAAGCGGTGGGAGGAGGCCACCGGCGAGCGAGTCGACGAGGTGATATTTCTGAGCAAACACACCGCCGTCTCCAACCGGCCGGCACTGACCGTGCACCCGATCGGAATCCCTCATCTGCGTGAAGGCGAGGCTGGACCGCAAGGCGGAAAGCCCGGTTGGGCTGCGCCGCCGAGCCCCCGGATTGGTCCCTGGCTCCGGCACCTGAAAAAGCTTGCCCAAGCCCACAACTTGGTTCCTGAGTTCGAGATTACTTTGGAGGGTACACATCATGGACCGGTCACGAATACACGTACTATGTTCTTGGAGATTGGCAGCACGGATGAGTGCTGGAAGAGGCAGGATGCAGCACAAGTTATGGCTCTATTAGTTTGGGAAGGACTTGGGCTTGGAGGGGGAGCTGCTGTTGGAAACTGGAACGGGGAGAAGGATAAGAATAAAGTTCTTCTTGGATTAGGTGGTGGACATTATGCTCCTCGGCATATGGATATAGTTCTGAAAGATGGTGTGTGGGTAGGCCATCTACTTTCGGGCTATTCCTTGCCGATGGAAGATCCAAACCAGTCCAAAGCAGAAACAAATGCAAAAGATACGAAAGACATTGGTGGAACTTGGAAACAAGCGATCAAAGCAGCATTTGAGGCTACCCAGTCTGCTTTCCCTGGTGGTGAAATTATAGCTCATCTTGATCAGAAGAGTTTCAAAAGCTGGCAAAAGAATGCTATAACGGCTTTCCTAGGCGAGCAGAACATCAAAATTGGCAAACCCAACGATTTCTACTAA

Protein Analysis

323

Amino Acids

34.74

Weight (kDa)

6.31

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 71 - 315 4.5e-56 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 387
AccB7I CCANNNNNTGG 1 cut(s) 418
AccI GTMKAC 1 cut(s) 222
AciI CCGC 5 cut(s) 193, 252, 326, 333, 356
AclWI GGATC 1 cut(s) 734
AcoI YGGCCR 2 cut(s) 51, 266
AfaI GTAC 2 cut(s) 451, 481
AfiI CCNNNNNNNGG 5 cut(s) 55, 332, 371, 384, 418
AflIII ACRYGT 1 cut(s) 476
AgeI ACCGGT 1 cut(s) 463
AgsI TTSAA 1 cut(s) 889
AjnI CCWGG 2 cut(s) 377, 847
AjuI GAANNNNNNNTTGG 2 cut(s) 328, 360
AluBI AGCT 4 cut(s) 400, 587, 866, 894
AluI AGCT 4 cut(s) 400, 587, 866, 894
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 262
Alw44I GTGCAC 1 cut(s) 281
AlwI GGATC 1 cut(s) 734
AoxI GGCC 6 cut(s) 36, 51, 57, 204, 266, 703
ApaLI GTGCAC 1 cut(s) 281
ApeKI GCWGC 6 cut(s) 151, 350, 501, 533, 587, 818
AsiGI ACCGGT 1 cut(s) 463
Asp700I GAANNNNTTC 1 cut(s) 884
AspA2I CCTAGG 1 cut(s) 922
AspLEI GCGC 1 cut(s) 355
AspS9I GGNCC 5 cut(s) 58, 82, 323, 374, 461
AsuC2I CCSGG 5 cut(s) 35, 50, 75, 342, 367
AsuHPI GGTGA 3 cut(s) 16, 241, 866
AvaII GGWCC 4 cut(s) 82, 323, 374, 461
AvrII CCTAGG 1 cut(s) 922
BaeGI GKGCMC 1 cut(s) 285
BanI GGYRCC 1 cut(s) 387
BanII GRGCYC 1 cut(s) 365
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 6 cut(s) 138, 337, 513, 545, 574, 830
BccI CCATC 3 cut(s) 683, 714, 727
BceAI ACGGC 2 cut(s) 239, 930
BciT130I CCWGG 2 cut(s) 379, 849
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 5 cut(s) 35, 50, 75, 342, 367
BcoDI GTCTC 1 cut(s) 262
BfaI CTAG 2 cut(s) 65, 923
BisI GCNGC 7 cut(s) 152, 351, 356, 502, 534, 588, 819
BlnI CCTAGG 1 cut(s) 922
BlsI GCNGC 7 cut(s) 153, 352, 357, 503, 535, 589, 820
Bme1390I CCNGG 7 cut(s) 35, 50, 75, 342, 367, 379, 849
Bme18I GGWCC 4 cut(s) 82, 323, 374, 461
BmgT120I GGNCC 5 cut(s) 58, 82, 323, 374, 461
BmiI GGNNCC 6 cut(s) 83, 84, 376, 383, 389, 423
BmrFI CCNGG 7 cut(s) 35, 50, 75, 342, 367, 379, 849
BmrI ACTGGG 1 cut(s) 829
BmsI GCATC 1 cut(s) 520
BmuI ACTGGG 1 cut(s) 829
BpiI GAAGAC 1 cut(s) 183
BpuMI CCSGG 5 cut(s) 35, 50, 75, 342, 367
BsaAI YACGTR 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 293
BsaJI CCNNGG 6 cut(s) 54, 365, 377, 662, 847, 922
BsaWI WCCGGW 1 cut(s) 463
BsaXI ACNNNNNCTCC 6 cut(s) 123, 129, 153, 159, 576, 606
Bsc4I CCNNNNNNNGG 5 cut(s) 55, 332, 371, 384, 418
Bse118I RCCGGY 4 cut(s) 209, 264, 268, 463
Bse1I ACTGG 3 cut(s) 605, 748, 835
Bse3DI GCAATG 1 cut(s) 130
Bse8I GATNNNNATC 1 cut(s) 293
BseBI CCWGG 2 cut(s) 379, 849
BseDI CCNNGG 6 cut(s) 54, 365, 377, 662, 847, 922
BseGI GGATG 2 cut(s) 514, 535
BseJI GATNNNNATC 1 cut(s) 293
BseLI CCNNNNNNNGG 5 cut(s) 55, 332, 371, 384, 418
BseMI GCAATG 1 cut(s) 130
BseMII CTCAG 2 cut(s) 230, 417
BseNI ACTGG 3 cut(s) 605, 748, 835
BseRI GAGGAG 5 cut(s) 144, 147, 150, 215, 651
BseSI GKGCMC 1 cut(s) 285
BseX3I CGGCCG 1 cut(s) 266
BseXI GCAGC 6 cut(s) 138, 337, 513, 545, 574, 830
BseYI CCCAGC 1 cut(s) 78
BsgI GTGCAG 1 cut(s) 137
Bsh1285I CGRYCG 2 cut(s) 269, 291
BshFI GGCC 6 cut(s) 38, 53, 59, 206, 268, 705
BshNI GGYRCC 1 cut(s) 387
BshTI ACCGGT 1 cut(s) 463
BsiEI CGRYCG 2 cut(s) 269, 291
BsiHKAI GWGCWC 1 cut(s) 285
BslFI GGGAC 2 cut(s) 95, 360
BslI CCNNNNNNNGG 5 cut(s) 55, 332, 371, 384, 418
BsmAI GTCTC 1 cut(s) 262
BsmBI CGTCTC 1 cut(s) 262
BsmFI GGGAC 2 cut(s) 95, 360
BsmI GAATGC 1 cut(s) 910
BsnI GGCC 6 cut(s) 38, 53, 59, 206, 268, 705
Bsp1286I GDGCHC 2 cut(s) 285, 365
Bsp143I GATC 5 cut(s) 12, 288, 739, 810, 874
BspACI CCGC 5 cut(s) 193, 252, 326, 333, 356
BspANI GGCC 6 cut(s) 38, 53, 59, 206, 268, 705
BspCNI CTCAG 2 cut(s) 231, 418
BspLI GGNNCC 6 cut(s) 83, 84, 376, 383, 389, 423
BspPI GGATC 1 cut(s) 734
BspT107I GGYRCC 1 cut(s) 387
BsrDI GCAATG 1 cut(s) 130
BsrFI RCCGGY 4 cut(s) 209, 264, 268, 463
BsrI ACTGG 3 cut(s) 605, 748, 835
BssAI RCCGGY 4 cut(s) 209, 264, 268, 463
BssECI CCNNGG 6 cut(s) 54, 365, 377, 662, 847, 922
BssMI GATC 5 cut(s) 12, 288, 739, 810, 874
BssT1I CCWWGG 2 cut(s) 54, 922
Bst2UI CCWGG 2 cut(s) 379, 849
Bst4CI ACNGT 2 cut(s) 158, 280
Bst6I CTCTTC 2 cut(s) 515, 875
BstBAI YACGTR 1 cut(s) 479
BstC8I GCNNGC 5 cut(s) 215, 270, 402, 896, 929
BstDEI CTNAG 2 cut(s) 239, 426
BstF5I GGATG 2 cut(s) 514, 535
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 5 cut(s) 15, 291, 742, 813, 877
BstMAI GTCTC 1 cut(s) 262
BstMBI GATC 5 cut(s) 12, 288, 739, 810, 874
BstMCI CGRYCG 2 cut(s) 269, 291
BstMWI GCNNNNNNNGC 4 cut(s) 65, 815, 827, 914
BstNI CCWGG 2 cut(s) 379, 849
BstSCI CCNGG 7 cut(s) 33, 48, 73, 340, 365, 377, 847
BstSLI GKGCMC 1 cut(s) 285
BstV1I GCAGC 6 cut(s) 138, 337, 513, 545, 574, 830
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 739
BstYI RGATCY 1 cut(s) 739
BstZI CGGCCG 1 cut(s) 266
BsuRI GGCC 6 cut(s) 38, 53, 59, 206, 268, 705
BtsCI GGATG 2 cut(s) 514, 535
BtsIMutI CAGTG 1 cut(s) 272
Cac8I GCNNGC 5 cut(s) 215, 270, 402, 896, 929
CfoI GCGC 1 cut(s) 355
Cfr10I RCCGGY 4 cut(s) 209, 264, 268, 463
Cfr13I GGNCC 5 cut(s) 58, 82, 323, 374, 461
CseI GACGC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 450, 480
CspAI ACCGGT 1 cut(s) 463
CviAII CATG 3 cut(s) 70, 109, 458
CviQI GTAC 2 cut(s) 450, 480
DdeI CTNAG 2 cut(s) 239, 426
DpnI GATC 5 cut(s) 14, 290, 741, 812, 876
DpnII GATC 5 cut(s) 12, 288, 739, 810, 874
EaeI YGGCCR 2 cut(s) 51, 266
EagI CGGCCG 1 cut(s) 266
Eam1104I CTCTTC 2 cut(s) 515, 875
EarI CTCTTC 2 cut(s) 515, 875
EciI GGCGGA 1 cut(s) 348
EclXI CGGCCG 1 cut(s) 266
Eco130I CCWWGG 2 cut(s) 54, 922
Eco24I GRGCYC 1 cut(s) 365
Eco47I GGWCC 4 cut(s) 82, 323, 374, 461
Eco52I CGGCCG 1 cut(s) 266
EcoO109I RGGNCCY 2 cut(s) 58, 82
EcoRII CCWGG 2 cut(s) 377, 847
EcoT14I CCWWGG 2 cut(s) 54, 922
EcoT38I GRGCYC 1 cut(s) 365
ErhI CCWWGG 2 cut(s) 54, 922
Esp3I CGTCTC 1 cut(s) 262
FaeI CATG 3 cut(s) 73, 112, 461
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 2 cut(s) 95, 360
FatI CATG 3 cut(s) 69, 108, 457
FauNDI CATATG 1 cut(s) 669
FbaI TGATCA 1 cut(s) 874
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 7 cut(s) 152, 351, 356, 502, 534, 588, 819
FokI GGATG 2 cut(s) 521, 542
FriOI GRGCYC 1 cut(s) 365
Fsp4HI GCNGC 7 cut(s) 152, 351, 356, 502, 534, 588, 819
FspBI CTAG 2 cut(s) 65, 923
GlaI GCGC 1 cut(s) 354
GluI GCNGC 7 cut(s) 152, 351, 356, 502, 534, 588, 819
GsaI CCCAGC 1 cut(s) 82
HaeIII GGCC 6 cut(s) 38, 53, 59, 206, 268, 705
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 355
Hin1II CATG 3 cut(s) 73, 112, 461
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HincII GTYRAC 2 cut(s) 46, 223
HindII GTYRAC 2 cut(s) 46, 223
HindIII AAGCTT 1 cut(s) 398
HinfI GANTC 3 cut(s) 138, 219, 294
HphI GGTGA 3 cut(s) 16, 241, 866
Hpy166II GTNNAC 6 cut(s) 46, 101, 223, 283, 452, 650
Hpy188I TCNGA 4 cut(s) 240, 293, 684, 879
Hpy188III TCNNGA 4 cut(s) 425, 433, 469, 872
Hpy8I GTNNAC 6 cut(s) 46, 101, 223, 283, 452, 650
Hpy99I CGWCG 2 cut(s) 25, 227
HpyAV CCTTC 5 cut(s) 102, 106, 305, 557, 607
HpyCH4III ACNGT 2 cut(s) 158, 280
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 4 cut(s) 154, 283, 533, 770
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 815, 827, 914
HpyF3I CTNAG 2 cut(s) 239, 426
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 3 cut(s) 73, 112, 461
HspAI GCGC 1 cut(s) 353
KflI GGGWCCC 1 cut(s) 82
KroI GCCGGC 1 cut(s) 268
KroNI GCCGGC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 874
Kzo9I GATC 5 cut(s) 12, 288, 739, 810, 874
LmnI GCTCC 3 cut(s) 387, 584, 664
Lsp1109I GCAGC 6 cut(s) 138, 337, 513, 545, 574, 830
LweI GCATC 1 cut(s) 520
MaeI CTAG 2 cut(s) 65, 923
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 2 cut(s) 4, 466
MalI GATC 5 cut(s) 14, 290, 741, 812, 876
MboI GATC 5 cut(s) 12, 288, 739, 810, 874
MboII GAAGA 6 cut(s) 185, 188, 532, 623, 749, 892
MflI RGATCY 1 cut(s) 739
MhlI GDGCHC 2 cut(s) 285, 365
MluCI AATT 3 cut(s) 165, 858, 942
MlyI GAGTC 2 cut(s) 147, 228
MmeI TCCRAC 2 cut(s) 285, 574
MroNI GCCGGC 1 cut(s) 268
MroXI GAANNNNTTC 1 cut(s) 884
MslI CAYNNNNRTG 1 cut(s) 306
MspR9I CCNGG 7 cut(s) 35, 50, 75, 342, 367, 379, 849
Mva1269I GAATGC 1 cut(s) 910
MvaI CCWGG 2 cut(s) 379, 849
MwoI GCNNNNNNNGC 4 cut(s) 65, 815, 827, 914
NaeI GCCGGC 1 cut(s) 270
NciI CCSGG 5 cut(s) 35, 50, 75, 342, 367
NdeI CATATG 1 cut(s) 669
NdeII GATC 5 cut(s) 12, 288, 739, 810, 874
NgoMIV GCCGGC 1 cut(s) 268
NlaIII CATG 3 cut(s) 73, 112, 461
NlaIV GGNNCC 6 cut(s) 83, 84, 376, 383, 389, 423
NmeAIII GCCGAG 2 cut(s) 384, 643
NmuCI GTSAC 2 cut(s) 4, 466
PctI GAATGC 1 cut(s) 910
PdiI GCCGGC 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 884
PfeI GAWTC 1 cut(s) 294
PflMI CCANNNNNTGG 1 cut(s) 418
PinAI ACCGGT 1 cut(s) 463
PkrI GCNGC 7 cut(s) 153, 352, 357, 503, 535, 589, 820
Ple19I CGATCG 1 cut(s) 291
PleI GAGTC 2 cut(s) 146, 227
PpsI GAGTC 2 cut(s) 146, 227
Ppu21I YACGTR 1 cut(s) 479
PpuMI RGGWCCY 1 cut(s) 82
Psp5II RGGWCCY 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 377, 847
PspFI CCCAGC 1 cut(s) 78
PspGI CCWGG 2 cut(s) 377, 847
PspN4I GGNNCC 6 cut(s) 83, 84, 376, 383, 389, 423
PspPI GGNCC 5 cut(s) 58, 82, 323, 374, 461
PspPPI RGGWCCY 1 cut(s) 82
PsuI RGATCY 1 cut(s) 739
PvuI CGATCG 1 cut(s) 291
RsaI GTAC 2 cut(s) 451, 481
RsaNI GTAC 2 cut(s) 450, 480
RseI CAYNNNNRTG 1 cut(s) 306
SalI GTCGAC 1 cut(s) 221
SatI GCNGC 7 cut(s) 152, 351, 356, 502, 534, 588, 819
Sau3AI GATC 5 cut(s) 12, 288, 739, 810, 874
Sau96I GGNCC 5 cut(s) 58, 82, 323, 374, 461
SchI GAGTC 2 cut(s) 147, 228
ScrFI CCNGG 7 cut(s) 35, 50, 75, 342, 367, 379, 849
SduI GDGCHC 2 cut(s) 285, 365
SetI ASST 8 cut(s) 231, 393, 402, 481, 589, 646, 868, 896
SfaNI GCATC 1 cut(s) 520
SgrAI CRCCGGYG 1 cut(s) 209
SinI GGWCC 4 cut(s) 82, 323, 374, 461
SmiMI CAYNNNNRTG 1 cut(s) 306
Sse9I AATT 3 cut(s) 165, 858, 942
SsiI CCGC 5 cut(s) 193, 252, 326, 333, 356
SspMI CTAG 2 cut(s) 65, 923
StyD4I CCNGG 7 cut(s) 33, 48, 73, 340, 365, 377, 847
StyI CCWWGG 2 cut(s) 54, 922
TaaI ACNGT 2 cut(s) 158, 280
TaiI ACGT 1 cut(s) 481
TaqI TCGA 2 cut(s) 222, 432
TasI AATT 3 cut(s) 165, 858, 942
TauI GCSGC 1 cut(s) 358
TfiI GAWTC 1 cut(s) 294
TscAI CASTG 1 cut(s) 279
TseFI GTSAC 2 cut(s) 4, 466
TseI GCWGC 6 cut(s) 151, 350, 501, 533, 587, 818
Tsp45I GTSAC 2 cut(s) 4, 466
TspDTI ATGAA 1 cut(s) 125
TspGWI ACGGA 1 cut(s) 521
TspRI CASTG 1 cut(s) 279
Van91I CCANNNNNTGG 1 cut(s) 418
VneI GTGCAC 1 cut(s) 281
VpaK11BI GGWCC 4 cut(s) 82, 323, 374, 461
XmaJI CCTAGG 1 cut(s) 922
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 1 cut(s) 884
XspI CTAG 2 cut(s) 65, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.