MD16G1055900.v1.1

D-aminoacyl-tRNA deacylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008523
Physical Location & Seq
Reverse (-)
3984124 .. 3986709
2586 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1055900.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGGCAAAGCTCCTCAACCCGACGTCGTTTAAGGCCATCTCTCACTTCATTGCCAGTCGCGGCAAAACAGTAAACAAAAGCATTTTTCCCCTCCGCAACGCTGCTAGCTGCACAGCCACAGAAACGACAAAGGAGATGGTGACGCTGATCGTGGCGACGACGACCGACCCGGCCTCCATCAACCCGGCCCATGCCCTCCTAGCCATGCCCGGGTGGGACCCCTCCCCTTCCTGTTTACAGGACATGAAGGGGTTTGCTAATGGAGGAGGAGGAGTGAGAGTTTTGCTACACAGCAGGGGAATTGTGGAAGAAGACGACTTGGACAGGCGGTGGGAGGAGGCCACCGGGGAGCGAGTCGACGAGGTGATATTCCTGAGCAAACACACCGCCGTCTCCAACCGGCCGGCACTGACGGTACACCCGATCGGAATCCCTCATCTGCGGGAAGGCGAGGCTGGGCCGCAAGGCGGGAAGCCGGGTTGGGCTGCGCCGCCAAGCCCCCGCATTGGACCCTGGCTCAGGCACCTGAAGAAGCTTGCCCAAGCCCACAACCTGGTTCCTGAGTTCGAGATTACATTGGAGGGTACGCATCATGGACCGGTAACGAATACGCCCACTATGTTCGTAGAGATTGGCAGTACAGATGAGTACTGGAAAAGGCAGGATGCAGCACAAGTTATTGCTCTATTGGTTTGGGAAGGACTTGGGCTTGGAGGAGGAGCTGCTGTTGGAAACTGGAACGGGGAGAAGGATAAGAATAAAATTCTTCTTGGATTAGGTGGTGGACATTATGCTCCTCGGCATATGGATATAGTCATGAAAGATGGGGTGTGGGTAGGCCATCTACTTTCGGGTTATTCCTTGCCGATGGAAGATCCAAATCAGTCCAAAGCAGGAACAAATGCAAAAGATACGAAGGACATTGGTGGAACTTGGAAACACGCAATCAAAGCAGCATTTGAGGCTACCCAGTCGGCTTTCCCTGGTGGGGAAGTTATAGCTCATCTTGATCAAAAGAGTTTTAAAAGCTGGCAAAAGAACGCTATAACGGCTTTCTTAGGCGAGCAGAACATCAAAATTGGCAAACCCAACGATTTCTACTAG

Protein Analysis

368

Amino Acids

39.63

Weight (kDa)

7.28

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA_deacylase PF04414 116 - 360 3.9e-58 D-aminoacyl-tRNA deacylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 522
AccB7I CCANNNNNTGG 1 cut(s) 553
AccI GTMKAC 1 cut(s) 357
AccII CGCG 1 cut(s) 60
AciI CCGC 9 cut(s) 60, 94, 328, 387, 442, 461, 468, 491, 502
AclWI GGATC 1 cut(s) 869
AcoI YGGCCR 1 cut(s) 401
AcsI RAATTY 1 cut(s) 762
AcuI CTGAAG 1 cut(s) 548
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 4 cut(s) 417, 586, 640, 650
AfiI CCNNNNNNNGG 6 cut(s) 210, 467, 506, 519, 553, 988
AgeI ACCGGT 1 cut(s) 598
AjnI CCWGG 3 cut(s) 512, 552, 982
AluBI AGCT 6 cut(s) 10, 108, 535, 722, 1001, 1029
AluI AGCT 6 cut(s) 10, 108, 535, 722, 1001, 1029
Alw26I GTCTC 1 cut(s) 397
AlwI GGATC 1 cut(s) 869
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 7 cut(s) 33, 171, 186, 339, 401, 458, 838
ApeKI GCWGC 6 cut(s) 101, 108, 485, 668, 722, 953
ApoI RAATTY 1 cut(s) 762
AsiGI ACCGGT 1 cut(s) 598
AspLEI GCGC 1 cut(s) 490
AspS9I GGNCC 5 cut(s) 187, 217, 458, 509, 596
AsuC2I CCSGG 6 cut(s) 170, 185, 210, 211, 346, 477
AsuHPI GGTGA 2 cut(s) 151, 376
AsuNHI GCTAGC 1 cut(s) 104
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 3 cut(s) 217, 509, 596
BanI GGYRCC 1 cut(s) 522
BbsI GAAGAC 1 cut(s) 318
BbvI GCAGC 6 cut(s) 88, 95, 472, 680, 709, 965
BccI CCATC 6 cut(s) 44, 130, 185, 818, 849, 862
BceAI ACGGC 2 cut(s) 374, 1065
BciT130I CCWGG 3 cut(s) 514, 554, 984
BclI TGATCA 1 cut(s) 1009
BcnI CCSGG 6 cut(s) 170, 185, 210, 211, 346, 477
BcoDI GTCTC 1 cut(s) 397
BfaI CTAG 3 cut(s) 105, 200, 1102
BisI GCNGC 9 cut(s) 61, 102, 109, 461, 486, 491, 669, 723, 954
BlsI GCNGC 9 cut(s) 62, 103, 110, 462, 487, 492, 670, 724, 955
BmcAI AGTACT 1 cut(s) 650
Bme1390I CCNGG 9 cut(s) 170, 185, 210, 211, 346, 477, 514, 554, 984
Bme18I GGWCC 3 cut(s) 217, 509, 596
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 5 cut(s) 187, 217, 458, 509, 596
BmiI GGNNCC 5 cut(s) 218, 219, 511, 524, 558
BmrFI CCNGG 9 cut(s) 170, 185, 210, 211, 346, 477, 514, 554, 984
BmrI ACTGGG 1 cut(s) 964
BmsI GCATC 2 cut(s) 598, 655
BmtI GCTAGC 1 cut(s) 108
BmuI ACTGGG 1 cut(s) 964
BpiI GAAGAC 1 cut(s) 318
Bpu10I CCTNAGC 2 cut(s) 374, 518
BpuMI CCSGG 6 cut(s) 170, 185, 210, 211, 346, 477
BsaBI GATNNNNATC 2 cut(s) 428, 879
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 209, 345, 512, 797, 982
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 8 cut(s) 158, 188, 258, 264, 288, 294, 711, 741
Bsc4I CCNNNNNNNGG 6 cut(s) 210, 467, 506, 519, 553, 988
Bse118I RCCGGY 3 cut(s) 399, 403, 598
Bse1I ACTGG 4 cut(s) 54, 656, 740, 970
Bse3DI GCAATG 1 cut(s) 48
Bse8I GATNNNNATC 2 cut(s) 428, 879
BseBI CCWGG 3 cut(s) 514, 554, 984
BseDI CCNNGG 5 cut(s) 209, 345, 512, 797, 982
BseGI GGATG 1 cut(s) 670
BseJI GATNNNNATC 2 cut(s) 428, 879
BseLI CCNNNNNNNGG 6 cut(s) 210, 467, 506, 519, 553, 988
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 3 cut(s) 365, 532, 552
BseNI ACTGG 4 cut(s) 54, 656, 740, 970
BseRI GAGGAG 7 cut(s) 279, 282, 285, 350, 729, 732, 786
BseX3I CGGCCG 1 cut(s) 401
BseXI GCAGC 6 cut(s) 88, 95, 472, 680, 709, 965
BseYI CCCAGC 1 cut(s) 455
BsgI GTGCAG 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 60
Bsh1285I CGRYCG 3 cut(s) 165, 404, 426
BshFI GGCC 7 cut(s) 35, 173, 188, 341, 403, 460, 840
BshNI GGYRCC 1 cut(s) 522
BshTI ACCGGT 1 cut(s) 598
BsiEI CGRYCG 3 cut(s) 165, 404, 426
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 8 cut(s) 170, 185, 210, 345, 400, 404, 476, 599
BslFI GGGAC 1 cut(s) 230
BslI CCNNNNNNNGG 6 cut(s) 210, 467, 506, 519, 553, 988
BsmAI GTCTC 1 cut(s) 397
BsmBI CGTCTC 1 cut(s) 397
BsmFI GGGAC 1 cut(s) 230
BsnI GGCC 7 cut(s) 35, 173, 188, 341, 403, 460, 840
BsoBI CYCGRG 1 cut(s) 209
Bsp143I GATC 4 cut(s) 147, 423, 874, 1009
BspACI CCGC 9 cut(s) 60, 94, 328, 387, 442, 461, 468, 491, 502
BspANI GGCC 7 cut(s) 35, 173, 188, 341, 403, 460, 840
BspCNI CTCAG 3 cut(s) 366, 531, 553
BspFNI CGCG 1 cut(s) 60
BspHI TCATGA 1 cut(s) 816
BspLI GGNNCC 5 cut(s) 218, 219, 511, 524, 558
BspOI GCTAGC 1 cut(s) 108
BspPI GGATC 1 cut(s) 869
BspT107I GGYRCC 1 cut(s) 522
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 3 cut(s) 399, 403, 598
BsrI ACTGG 4 cut(s) 54, 656, 740, 970
BssAI RCCGGY 3 cut(s) 399, 403, 598
BssECI CCNNGG 5 cut(s) 209, 345, 512, 797, 982
BssMI GATC 4 cut(s) 147, 423, 874, 1009
BssNI GRCGYC 1 cut(s) 23
Bst2UI CCWGG 3 cut(s) 514, 554, 984
Bst4CI ACNGT 2 cut(s) 70, 415
BstACI GRCGYC 1 cut(s) 23
BstC8I GCNNGC 5 cut(s) 106, 405, 537, 1031, 1064
BstDEI CTNAG 4 cut(s) 374, 518, 561, 1057
BstENI CCTNNNNNAGG 1 cut(s) 517
BstF5I GGATG 1 cut(s) 670
BstFNI CGCG 1 cut(s) 60
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 4 cut(s) 150, 426, 877, 1012
BstMAI GTCTC 1 cut(s) 397
BstMBI GATC 4 cut(s) 147, 423, 874, 1009
BstMCI CGRYCG 3 cut(s) 165, 404, 426
BstMWI GCNNNNNNNGC 4 cut(s) 200, 950, 962, 1049
BstNI CCWGG 3 cut(s) 514, 554, 984
BstSCI CCNGG 9 cut(s) 168, 183, 208, 209, 344, 475, 512, 552, 982
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 6 cut(s) 88, 95, 472, 680, 709, 965
BstV2I GAAGAC 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 874
BstYI RGATCY 1 cut(s) 874
BstZI CGGCCG 1 cut(s) 401
BsuRI GGCC 7 cut(s) 35, 173, 188, 341, 403, 460, 840
BtsCI GGATG 1 cut(s) 670
BtsIMutI CAGTG 1 cut(s) 407
Cac8I GCNNGC 5 cut(s) 106, 405, 537, 1031, 1064
CciI TCATGA 1 cut(s) 816
CfoI GCGC 1 cut(s) 490
Cfr10I RCCGGY 3 cut(s) 399, 403, 598
Cfr13I GGNCC 5 cut(s) 187, 217, 458, 509, 596
Cfr9I CCCGGG 1 cut(s) 209
CseI GACGC 1 cut(s) 151
CsiI ACCWGGT 1 cut(s) 552
Csp6I GTAC 4 cut(s) 416, 585, 639, 649
CspAI ACCGGT 1 cut(s) 598
CviAII CATG 5 cut(s) 191, 205, 244, 593, 817
CviQI GTAC 4 cut(s) 416, 585, 639, 649
DdeI CTNAG 4 cut(s) 374, 518, 561, 1057
DpnI GATC 4 cut(s) 149, 425, 876, 1011
DpnII GATC 4 cut(s) 147, 423, 874, 1009
DraI TTTAAA 1 cut(s) 1024
EaeI YGGCCR 1 cut(s) 401
EagI CGGCCG 1 cut(s) 401
EclXI CGGCCG 1 cut(s) 401
Eco47I GGWCC 3 cut(s) 217, 509, 596
Eco52I CGGCCG 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 209
EcoNI CCTNNNNNAGG 1 cut(s) 517
EcoO109I RGGNCCY 1 cut(s) 217
EcoRII CCWGG 3 cut(s) 512, 552, 982
Esp3I CGTCTC 1 cut(s) 397
FaeI CATG 5 cut(s) 194, 208, 247, 596, 820
FaqI GGGAC 1 cut(s) 230
FatI CATG 5 cut(s) 190, 204, 243, 592, 816
FauI CCCGC 3 cut(s) 435, 461, 509
FauNDI CATATG 1 cut(s) 804
FbaI TGATCA 1 cut(s) 1009
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 9 cut(s) 61, 102, 109, 461, 486, 491, 669, 723, 954
FokI GGATG 1 cut(s) 677
Fsp4HI GCNGC 9 cut(s) 61, 102, 109, 461, 486, 491, 669, 723, 954
FspBI CTAG 3 cut(s) 105, 200, 1102
GlaI GCGC 1 cut(s) 489
GluI GCNGC 9 cut(s) 61, 102, 109, 461, 486, 491, 669, 723, 954
GsaI CCCAGC 1 cut(s) 459
HaeIII GGCC 7 cut(s) 35, 173, 188, 341, 403, 460, 840
HapII CCGG 8 cut(s) 170, 185, 210, 345, 400, 404, 476, 599
HgaI GACGC 1 cut(s) 151
HhaI GCGC 1 cut(s) 490
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 5 cut(s) 194, 208, 247, 596, 820
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HincII GTYRAC 1 cut(s) 358
HindII GTYRAC 1 cut(s) 358
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 2 cut(s) 354, 429
HpaII CCGG 8 cut(s) 170, 185, 210, 345, 400, 404, 476, 599
HphI GGTGA 2 cut(s) 151, 376
Hpy166II GTNNAC 5 cut(s) 73, 236, 358, 418, 785
Hpy188I TCNGA 1 cut(s) 428
Hpy188III TCNNGA 5 cut(s) 373, 560, 568, 817, 1007
Hpy8I GTNNAC 5 cut(s) 73, 236, 358, 418, 785
Hpy99I CGWCG 5 cut(s) 25, 28, 160, 163, 362
HpyAV CCTTC 6 cut(s) 237, 241, 440, 692, 742, 910
HpyCH4III ACNGT 2 cut(s) 70, 415
HpyCH4IV ACGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 111, 668, 905
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 950, 962, 1049
HpyF3I CTNAG 4 cut(s) 374, 518, 561, 1057
HpySE526I ACGT 1 cut(s) 23
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 5 cut(s) 194, 208, 247, 596, 820
HspAI GCGC 1 cut(s) 488
KflI GGGWCCC 1 cut(s) 217
KroI GCCGGC 1 cut(s) 403
KroNI GCCGGC 1 cut(s) 405
Ksp22I TGATCA 1 cut(s) 1009
Kzo9I GATC 4 cut(s) 147, 423, 874, 1009
LmnI GCTCC 4 cut(s) 15, 349, 719, 799
Lsp1109I GCAGC 6 cut(s) 88, 95, 472, 680, 709, 965
LweI GCATC 2 cut(s) 598, 655
MabI ACCWGGT 1 cut(s) 552
MaeI CTAG 3 cut(s) 105, 200, 1102
MaeII ACGT 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 139, 601
MalI GATC 4 cut(s) 149, 425, 876, 1011
MboI GATC 4 cut(s) 147, 423, 874, 1009
MboII GAAGA 5 cut(s) 320, 323, 541, 758, 884
MflI RGATCY 1 cut(s) 874
MluCI AATT 3 cut(s) 300, 762, 1077
MlyI GAGTC 1 cut(s) 363
MmeI TCCRAC 2 cut(s) 420, 709
MroNI GCCGGC 1 cut(s) 403
MseI TTAA 2 cut(s) 30, 1023
MspI CCGG 8 cut(s) 170, 185, 210, 345, 400, 404, 476, 599
MspR9I CCNGG 9 cut(s) 170, 185, 210, 211, 346, 477, 514, 554, 984
MvaI CCWGG 3 cut(s) 514, 554, 984
MvnI CGCG 1 cut(s) 60
MwoI GCNNNNNNNGC 4 cut(s) 200, 950, 962, 1049
NaeI GCCGGC 1 cut(s) 405
NciI CCSGG 6 cut(s) 170, 185, 210, 211, 346, 477
NdeI CATATG 1 cut(s) 804
NdeII GATC 4 cut(s) 147, 423, 874, 1009
NgoMIV GCCGGC 1 cut(s) 403
NheI GCTAGC 1 cut(s) 104
NlaIII CATG 5 cut(s) 194, 208, 247, 596, 820
NlaIV GGNNCC 5 cut(s) 218, 219, 511, 524, 558
NmeAIII GCCGAG 1 cut(s) 778
NmuCI GTSAC 1 cut(s) 139
PagI TCATGA 1 cut(s) 816
PcsI WCGNNNNNNNCGW 1 cut(s) 419
PdiI GCCGGC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 429
PflMI CCANNNNNTGG 1 cut(s) 553
PinAI ACCGGT 1 cut(s) 598
PkrI GCNGC 9 cut(s) 62, 103, 110, 462, 487, 492, 670, 724, 955
Ple19I CGATCG 1 cut(s) 426
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
PpuMI RGGWCCY 1 cut(s) 217
Psp5II RGGWCCY 1 cut(s) 217
Psp6I CCWGG 3 cut(s) 512, 552, 982
PspFI CCCAGC 1 cut(s) 455
PspGI CCWGG 3 cut(s) 512, 552, 982
PspN4I GGNNCC 5 cut(s) 218, 219, 511, 524, 558
PspPI GGNCC 5 cut(s) 187, 217, 458, 509, 596
PspPPI RGGWCCY 1 cut(s) 217
PsuI RGATCY 1 cut(s) 874
PvuI CGATCG 1 cut(s) 426
RsaI GTAC 4 cut(s) 417, 586, 640, 650
RsaNI GTAC 4 cut(s) 416, 585, 639, 649
SalI GTCGAC 1 cut(s) 356
SaqAI TTAA 2 cut(s) 30, 1023
SatI GCNGC 9 cut(s) 61, 102, 109, 461, 486, 491, 669, 723, 954
Sau3AI GATC 4 cut(s) 147, 423, 874, 1009
Sau96I GGNCC 5 cut(s) 187, 217, 458, 509, 596
ScaI AGTACT 1 cut(s) 650
SchI GAGTC 1 cut(s) 363
ScrFI CCNGG 9 cut(s) 170, 185, 210, 211, 346, 477, 514, 554, 984
SexAI ACCWGGT 1 cut(s) 552
SfaNI GCATC 2 cut(s) 598, 655
SinI GGWCC 3 cut(s) 217, 509, 596
SmaI CCCGGG 1 cut(s) 211
Sse9I AATT 3 cut(s) 300, 762, 1077
SsiI CCGC 9 cut(s) 60, 94, 328, 387, 442, 461, 468, 491, 502
SspMI CTAG 3 cut(s) 105, 200, 1102
StyD4I CCNGG 9 cut(s) 168, 183, 208, 209, 344, 475, 512, 552, 982
TaaI ACNGT 2 cut(s) 70, 415
TaiI ACGT 1 cut(s) 26
TaqI TCGA 2 cut(s) 357, 567
TaqII GACCGA 1 cut(s) 179
TasI AATT 3 cut(s) 300, 762, 1077
TatI WGTACW 2 cut(s) 638, 648
TauI GCSGC 3 cut(s) 63, 463, 493
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 2 cut(s) 30, 1023
Tru9I TTAA 2 cut(s) 30, 1023
TscAI CASTG 1 cut(s) 414
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 6 cut(s) 101, 108, 485, 668, 722, 953
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 3 cut(s) 37, 260, 833
TspMI CCCGGG 1 cut(s) 209
TspRI CASTG 1 cut(s) 414
Van91I CCANNNNNTGG 1 cut(s) 553
VpaK11BI GGWCC 3 cut(s) 217, 509, 596
XagI CCTNNNNNAGG 1 cut(s) 517
XapI RAATTY 1 cut(s) 762
XmaI CCCGGG 1 cut(s) 209
XmiI GTMKAC 1 cut(s) 357
XspI CTAG 3 cut(s) 105, 200, 1102
ZraI GACGTC 1 cut(s) 24
ZrmI AGTACT 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.