AT2G14835

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009444
Physical Location & Seq
Forward (+)
6366879 .. 6371126
4248 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G14835.1

Sequence Viewer

Length: 1032 bp
ATGGTGGTCTGCAAATGCAAAAAGGCTACCAGGCTGTATTGCTTTGTTCACAAGGCCCCTGTTTGCGGCGAATGCATTTGTTTCCCGGAGCATCAAACTTGTGTGGTCCGGACGTATTCAGAATGGGTGATAGATGGAGAGTATGATCAGCCAAAGTGTTGTCAATGCCAAGCAACATTTGATGAGGGGGCAGGTCATCAAGTCACTCGGTTAGGTTGCTTGCATGCTATACATACAAGCTGCCTGGTTTCGCTTATCAAGAGTTTTCCTCCTCATACTGCACCTGCTGGTTATGTGTGCCCAGCATGTAGCACACCTATATGGCCTCCTATGATGGTAAAAGATGCAGGATCTCGGCTTCATGCTCTGTTAAGGGAAGTAATTACGCAGACTGGTCTTGAGAAGAATTTACTTGGGAACCATCCAGTTTCTCGATCCACCGAATCTCGTAGCCCACCACCGGCATTTGCTTCGGATGCATTAATTAATATATCATCATCTTCTCATACACAAGAAGGGAATAACCTTCCTGATGGTTATTCAGTAGCTGGAAATGGAGAATATTCCAAATCTGCGGTTTCAGAGATAGTTGAGATAGATGTTCCTGCTTCTGCTGGGAGTTACATGAAAAGCTCAAGCCCTGGACTTGCTGCTGCTGCTGCACGGAAAGGAGTACCCGCTGTAGACAGGCAGAACTCCGAGACTCTATATTATGCAGACGATGAAGACGGCAATCGTAAAAAGTACTCAAGAAGAGGTCCTCTTCGTCACAAGTTTCTACGAGCTTTACTACCTTTCTGGTCTAGTGCATTACCAACTCTACCCGTGACAGCACCACCTCGTAAGGATGCAGCAAAGGCAGATGATGGTTCTGAAGGTCGTGTAAGGCATCAACGATCATCAAAAATGGATATAAGGAAGATACTAATTTTCATAGCTCTCATAGCGTGTATGGCAACAATGGGGATTTTATACTACAGACTCGCACTACAGGCAATTGGTCAAGAACTACCCGATGAGGAGCAGCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

343

Amino Acids

37.44

Weight (kDa)

8.44

Isoelectric Point (pI)

57.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box_ZFPL1 PF25993 1 - 45 5.2e-21 ZFPL1-like, B-box zinc-binding domain
U-box_ZFPL1 PF25998 52 - 128 5.5e-38 ZFPL1-like, U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 292
Acc36I ACCTGC 2 cut(s) 182, 292
AccI GTMKAC 1 cut(s) 684
AccIII TCCGGA 1 cut(s) 108
AciI CCGC 3 cut(s) 66, 575, 678
AclWI GGATC 2 cut(s) 358, 429
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 2 cut(s) 675, 746
AfiI CCNNNNNNNGG 2 cut(s) 65, 460
AjnI CCWGG 3 cut(s) 29, 243, 640
AluBI AGCT 5 cut(s) 240, 548, 633, 785, 938
AluI AGCT 5 cut(s) 240, 548, 633, 785, 938
Alw26I GTCTC 1 cut(s) 695
AlwI GGATC 2 cut(s) 358, 429
AlwNI CAGNNNCTG 1 cut(s) 548
Aor13HI TCCGGA 1 cut(s) 108
AoxI GGCC 2 cut(s) 54, 323
ApeKI GCWGC 7 cut(s) 240, 650, 653, 656, 659, 851, 1024
ApoI RAATTY 1 cut(s) 406
AseI ATTAAT 2 cut(s) 482, 486
AspS9I GGNCC 3 cut(s) 55, 106, 758
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 139
AvaII GGWCC 2 cut(s) 106, 758
BaeGI GKGCMC 1 cut(s) 302
BbsI GAAGAC 1 cut(s) 732
BbvI GCAGC 6 cut(s) 227, 637, 640, 643, 646, 863
BccI CCATC 5 cut(s) 128, 328, 429, 527, 860
BceAI ACGGC 1 cut(s) 745
BcgI CGANNNNNNTGC 2 cut(s) 453, 487
BciT130I CCWGG 3 cut(s) 31, 245, 642
BclI TGATCA 1 cut(s) 145
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 695
BfaI CTAG 1 cut(s) 804
BfmI CTRYAG 3 cut(s) 681, 976, 989
BfuAI ACCTGC 2 cut(s) 182, 292
BisI GCNGC 8 cut(s) 67, 241, 651, 654, 657, 660, 852, 1025
BlsI GCNGC 8 cut(s) 68, 242, 652, 655, 658, 661, 853, 1026
BmcAI AGTACT 1 cut(s) 746
Bme1390I CCNGG 4 cut(s) 31, 86, 245, 642
Bme18I GGWCC 2 cut(s) 106, 758
BmgT120I GGNCC 3 cut(s) 55, 106, 758
BmiI GGNNCC 2 cut(s) 57, 419
BmrFI CCNGG 4 cut(s) 31, 86, 245, 642
BmsI GCATC 5 cut(s) 100, 334, 466, 838, 898
BpiI GAAGAC 1 cut(s) 732
BplI GAGNNNNNCTC 2 cut(s) 253, 285
BpuEI CTTGAG 3 cut(s) 419, 619, 733
BpuMI CCSGG 1 cut(s) 86
BsaJI CCNNGG 1 cut(s) 640
BsaWI WCCGGW 1 cut(s) 108
Bsc4I CCNNNNNNNGG 2 cut(s) 65, 460
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 397, 425
BseAI TCCGGA 1 cut(s) 108
BseBI CCWGG 3 cut(s) 31, 245, 642
BseDI CCNNGG 1 cut(s) 640
BseGI GGATG 3 cut(s) 421, 481, 853
BseLI CCNNNNNNNGG 2 cut(s) 65, 460
BseNI ACTGG 2 cut(s) 397, 425
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 302
BseXI GCAGC 6 cut(s) 227, 637, 640, 643, 646, 863
BseYI CCCAGC 2 cut(s) 301, 614
BsgI GTGCAG 2 cut(s) 264, 645
BshFI GGCC 2 cut(s) 56, 325
BsiSI CCGG 3 cut(s) 86, 109, 461
BslI CCNNNNNNNGG 2 cut(s) 65, 460
BsmAI GTCTC 1 cut(s) 695
BsmI GAATGC 1 cut(s) 77
BsnI GGCC 2 cut(s) 56, 325
Bsp1286I GDGCHC 1 cut(s) 302
Bsp13I TCCGGA 1 cut(s) 108
Bsp143I GATC 4 cut(s) 145, 350, 434, 896
BspACI CCGC 3 cut(s) 66, 575, 678
BspANI GGCC 2 cut(s) 56, 325
BspEI TCCGGA 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 57, 419
BspMI ACCTGC 2 cut(s) 182, 292
BspPI GGATC 2 cut(s) 358, 429
BsrFI RCCGGY 1 cut(s) 460
BsrI ACTGG 2 cut(s) 397, 425
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 1 cut(s) 640
BssMI GATC 4 cut(s) 145, 350, 434, 896
Bst2UI CCWGG 3 cut(s) 31, 245, 642
Bst6I CTCTTC 2 cut(s) 748, 768
BstC8I GCNNGC 2 cut(s) 221, 225
BstF5I GGATG 3 cut(s) 421, 481, 853
BstKTI GATC 4 cut(s) 148, 353, 437, 899
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 4 cut(s) 145, 350, 434, 896
BstMWI GCNNNNNNNGC 8 cut(s) 72, 476, 656, 659, 857, 944, 953, 992
BstNI CCWGG 3 cut(s) 31, 245, 642
BstNSI RCATGY 2 cut(s) 227, 309
BstSCI CCNGG 4 cut(s) 29, 84, 243, 640
BstSFI CTRYAG 3 cut(s) 681, 976, 989
BstSLI GKGCMC 1 cut(s) 302
BstV1I GCAGC 6 cut(s) 227, 637, 640, 643, 646, 863
BstV2I GAAGAC 1 cut(s) 732
BstX2I RGATCY 1 cut(s) 350
BstYI RGATCY 1 cut(s) 350
BsuRI GGCC 2 cut(s) 56, 325
BtsCI GGATG 3 cut(s) 421, 481, 853
BveI ACCTGC 2 cut(s) 182, 292
Cac8I GCNNGC 2 cut(s) 221, 225
CaiI CAGNNNCTG 1 cut(s) 548
Cfr10I RCCGGY 1 cut(s) 460
Cfr13I GGNCC 3 cut(s) 55, 106, 758
Csp6I GTAC 2 cut(s) 674, 745
CviAII CATG 4 cut(s) 224, 306, 362, 625
CviQI GTAC 2 cut(s) 674, 745
DpnI GATC 4 cut(s) 147, 352, 436, 898
DpnII GATC 4 cut(s) 145, 350, 434, 896
Eam1104I CTCTTC 2 cut(s) 748, 768
EarI CTCTTC 2 cut(s) 748, 768
Eco47I GGWCC 2 cut(s) 106, 758
Eco57I CTGAAG 1 cut(s) 894
EcoO109I RGGNCCY 2 cut(s) 55, 758
EcoRII CCWGG 3 cut(s) 29, 243, 640
EcoT22I ATGCAT 2 cut(s) 77, 481
FaeI CATG 4 cut(s) 227, 309, 365, 628
FatI CATG 4 cut(s) 223, 305, 361, 624
FauI CCCGC 1 cut(s) 685
FbaI TGATCA 1 cut(s) 145
FblI GTMKAC 1 cut(s) 684
Fnu4HI GCNGC 8 cut(s) 67, 241, 651, 654, 657, 660, 852, 1025
FokI GGATG 3 cut(s) 408, 488, 860
Fsp4HI GCNGC 8 cut(s) 67, 241, 651, 654, 657, 660, 852, 1025
FspBI CTAG 1 cut(s) 804
GluI GCNGC 8 cut(s) 67, 241, 651, 654, 657, 660, 852, 1025
GsaI CCCAGC 2 cut(s) 305, 618
HaeIII GGCC 2 cut(s) 56, 325
HapII CCGG 3 cut(s) 86, 109, 461
Hin1II CATG 4 cut(s) 227, 309, 365, 628
HinfI GANTC 3 cut(s) 443, 703, 981
HpaII CCGG 3 cut(s) 86, 109, 461
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 2 cut(s) 49, 685
Hpy188I TCNGA 5 cut(s) 121, 475, 583, 700, 874
Hpy188III TCNNGA 7 cut(s) 109, 259, 398, 432, 530, 750, 1004
Hpy8I GTNNAC 2 cut(s) 49, 685
HpyAV CCTTC 3 cut(s) 509, 536, 869
HpyCH4IV ACGT 1 cut(s) 113
HpyF10VI GCNNNNNNNGC 8 cut(s) 72, 476, 656, 659, 857, 944, 953, 992
HpySE526I ACGT 1 cut(s) 113
Hsp92II CATG 4 cut(s) 227, 309, 365, 628
Kpn2I TCCGGA 1 cut(s) 108
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 4 cut(s) 145, 350, 434, 896
LmnI GCTCC 2 cut(s) 88, 1021
Lsp1109I GCAGC 6 cut(s) 227, 637, 640, 643, 646, 863
LweI GCATC 5 cut(s) 100, 334, 466, 838, 898
MaeI CTAG 1 cut(s) 804
MaeII ACGT 1 cut(s) 113
MaeIII GTNAC 4 cut(s) 202, 620, 767, 826
MalI GATC 4 cut(s) 147, 352, 436, 898
MboI GATC 4 cut(s) 145, 350, 434, 896
MboII GAAGA 6 cut(s) 415, 492, 737, 755, 765, 931
MfeI CAATTG 1 cut(s) 996
MflI RGATCY 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 302
MluCI AATT 5 cut(s) 381, 406, 483, 927, 996
MlyI GAGTC 2 cut(s) 697, 975
MnlI CCTC 8 cut(s) 178, 279, 282, 336, 749, 771, 849, 1012
Mph1103I ATGCAT 2 cut(s) 77, 481
MroI TCCGGA 1 cut(s) 108
MseI TTAA 3 cut(s) 371, 482, 486
MslI CAYNNNNRTG 1 cut(s) 319
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 3 cut(s) 86, 109, 461
MspR9I CCNGG 4 cut(s) 31, 86, 245, 642
MunI CAATTG 1 cut(s) 996
Mva1269I GAATGC 1 cut(s) 77
MvaI CCWGG 3 cut(s) 31, 245, 642
MwoI GCNNNNNNNGC 8 cut(s) 72, 476, 656, 659, 857, 944, 953, 992
NciI CCSGG 1 cut(s) 86
NdeII GATC 4 cut(s) 145, 350, 434, 896
NlaIII CATG 4 cut(s) 227, 309, 365, 628
NlaIV GGNNCC 2 cut(s) 57, 419
NmeAIII GCCGAG 1 cut(s) 334
NmuCI GTSAC 3 cut(s) 202, 767, 826
NsiI ATGCAT 2 cut(s) 77, 481
NspI RCATGY 2 cut(s) 227, 309
PacI TTAATTAA 1 cut(s) 486
PaeI GCATGC 1 cut(s) 227
PaqCI CACCTGC 1 cut(s) 292
PctI GAATGC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 443
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 8 cut(s) 68, 242, 652, 655, 658, 661, 853, 1026
PleI GAGTC 2 cut(s) 697, 975
PpsI GAGTC 2 cut(s) 697, 975
PpuMI RGGWCCY 1 cut(s) 758
PshBI ATTAAT 2 cut(s) 482, 486
Psp5II RGGWCCY 1 cut(s) 758
Psp6I CCWGG 3 cut(s) 29, 243, 640
PspFI CCCAGC 2 cut(s) 301, 614
PspGI CCWGG 3 cut(s) 29, 243, 640
PspN4I GGNNCC 2 cut(s) 57, 419
PspPI GGNCC 3 cut(s) 55, 106, 758
PspPPI RGGWCCY 1 cut(s) 758
PstNI CAGNNNCTG 1 cut(s) 548
PsuI RGATCY 1 cut(s) 350
RsaI GTAC 2 cut(s) 675, 746
RsaNI GTAC 2 cut(s) 674, 745
RseI CAYNNNNRTG 1 cut(s) 319
SaqAI TTAA 3 cut(s) 371, 482, 486
SatI GCNGC 8 cut(s) 67, 241, 651, 654, 657, 660, 852, 1025
Sau3AI GATC 4 cut(s) 145, 350, 434, 896
Sau96I GGNCC 3 cut(s) 55, 106, 758
ScaI AGTACT 1 cut(s) 746
SchI GAGTC 2 cut(s) 697, 975
ScrFI CCNGG 4 cut(s) 31, 86, 245, 642
SduI GDGCHC 1 cut(s) 302
SfaNI GCATC 5 cut(s) 100, 334, 466, 838, 898
SfcI CTRYAG 3 cut(s) 681, 976, 989
SinI GGWCC 2 cut(s) 106, 758
SmiMI CAYNNNNRTG 1 cut(s) 319
SmlI CTYRAG 3 cut(s) 398, 634, 748
SmoI CTYRAG 3 cut(s) 398, 634, 748
SphI GCATGC 1 cut(s) 227
Sse9I AATT 5 cut(s) 381, 406, 483, 927, 996
SsiI CCGC 3 cut(s) 66, 575, 678
SspI AATATT 1 cut(s) 563
SspMI CTAG 1 cut(s) 804
StyD4I CCNGG 4 cut(s) 29, 84, 243, 640
TaiI ACGT 1 cut(s) 116
TaqI TCGA 1 cut(s) 433
TasI AATT 5 cut(s) 381, 406, 483, 927, 996
TatI WGTACW 1 cut(s) 744
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 3 cut(s) 371, 482, 486
Tru9I TTAA 3 cut(s) 371, 482, 486
TseFI GTSAC 3 cut(s) 202, 767, 826
TseI GCWGC 7 cut(s) 240, 650, 653, 656, 659, 851, 1024
Tsp45I GTSAC 3 cut(s) 202, 767, 826
TspDTI ATGAA 4 cut(s) 350, 641, 738, 922
TspGWI ACGGA 1 cut(s) 679
VpaK11BI GGWCC 2 cut(s) 106, 758
VspI ATTAAT 2 cut(s) 482, 486
XapI RAATTY 1 cut(s) 406
XceI RCATGY 2 cut(s) 227, 309
XmiI GTMKAC 1 cut(s) 684
XspI CTAG 1 cut(s) 804
ZrmI AGTACT 1 cut(s) 746
Zsp2I ATGCAT 2 cut(s) 77, 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.